[
    {
        "id": "authors:e5jk7-nbb83",
        "collection": "authors",
        "collection_id": "e5jk7-nbb83",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160216-101221139",
        "type": "article",
        "title": "Removal of sodium dodecyl sulfate from protein and peptide samples with cross-linked [Os(dmebpy)_2Cl]^(+/2+)-derivatized acrylamide and vinylimidazole copolymer",
        "author": [
            {
                "family_name": "Zhou",
                "given_name": "Jie",
                "clpid": "Zhou-Jie"
            },
            {
                "family_name": "Rusnak",
                "given_name": "Felicia",
                "clpid": "Rusnak-F"
            }
        ],
        "abstract": "Rationale\nSodium dodecyl sulfate (SDS) is widely used for the solubilization and denaturation of proteins, but it interferes with liquid chromatography/mass spectrometry (LC/MS), suppressing protein signals or forming adduct ions. A quick and effective clean-up technique of SDS is essential for MS analysis of proteins. Ion-exchange spin columns are commonly used for SDS removal in protein samples.\nMethods\nA bulk sample of insoluble, cross-linked [Os(dimethylbipyridine)_2Cl]^(+/2+)-derivatized poly(acrylamide)-poly(vinylimidazole) copolymer was synthesized and broken into small particles. The polymer was activated by washing with 1:1 ACN/water 50 mM triethylammonium phosphate 0.05% TFA, 0.1% TFA ACN and then 0.1% TFA water. Under acidic aqueous conditions, SDS adsorbs on the activated surfaces of the Os-complexed copolymer particles, but not the proteins and peptides in the same mixtures. Thus, the copolymer can be used to remove SDS from protein and peptide samples. The copolymer-adsorbed SDS is removed by washing with 0.1% TFA ACN, permitting re-use of the copolymer.\nResults\nStandard myoglobin and some practical protein samples from a biochemistry lab spiked with different concentrations of SDS were successfully cleaned up using this Os-copolymer for LC/MS analyses. Up to 0.2% (w/v %) of SDS can be successfully removed from those protein samples.\nConclusions\nThis Os-complexed copolymer provides a new alternative for quick cleanup of SDS from protein samples, and can serve as a new class of metal complex based anion exchanger for protein purification.",
        "doi": "10.1002/rcm.7478",
        "issn": "0951-4198",
        "publisher": "Wiley",
        "publication": "Rapid Communications in Mass Spectrometry",
        "publication_date": "2016-03-30",
        "series_number": "6",
        "volume": "30",
        "issue": "6",
        "pages": "698-704"
    }
]