[ { "id": "authors:z24va-45a37", "collection": "authors", "collection_id": "z24va-45a37", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200409-115737860", "type": "book_section", "title": "Comparative analysis of human and mouse development: From zygote to pre-gastrulation", "book_title": "Gastrulation: From Embryonic Pattern to Form", "author": [ { "family_name": "Mol\u00e8", "given_name": "Matteo A.", "clpid": "Mol\u00e8-M-A" }, { "family_name": "Weberling", "given_name": "Antonia", "clpid": "Weberling-A" }, { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" } ], "contributor": [ { "family_name": "Solnica-Krezel", "given_name": "Lilianna", "clpid": "Solnica-Krezel-L" } ], "abstract": "Development of the mammalian embryo begins with formation of the totipotent zygote during fertilization. This initial cell is able to give rise to every embryonic tissue of the developing organism as well as all extra-embryonic lineages, such as the placenta and the yolk sac, which are essential for the initial patterning and support growth of the fetus until birth. As the embryo transits from pre- to post-implantation, major structural and transcriptional changes occur within the embryonic lineage to set up the basis for the subsequent phase of gastrulation. Fine-tuned coordination of cell division, morphogenesis and differentiation is essential to ultimately promote assembly of the future fetus. Here, we review the current knowledge of mammalian development of both mouse and human focusing on morphogenetic processes leading to the onset of gastrulation, when the embryonic anterior-posterior axis becomes established and the three germ layers start to be specified.", "doi": "10.1016/bs.ctdb.2019.10.002", "isbn": "9780128127988", "publisher": "Elsevier", "place_of_publication": "Cambridge, MA", "publication_date": "2020", "pages": "113-138" }, { "id": "authors:c26rd-z4628", "collection": "authors", "collection_id": "c26rd-z4628", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190408-161504340", "type": "book_section", "title": "Delivery of mtZFNs into Early Mouse Embryos", "book_title": "Zinc Finger Proteins: Methods and Protocols", "author": [ { "family_name": "McCann", "given_name": "Beverly J.", "clpid": "McCann-B-J" }, { "family_name": "Cox", "given_name": "Andy", "clpid": "Cox-Andy" }, { "family_name": "Gammage", "given_name": "Payam A.", "clpid": "Gammage-P-A" }, { "family_name": "Stewart", "given_name": "James B.", "clpid": "Stewart-J-B" }, { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" }, { "family_name": "Minczuk", "given_name": "Michal", "clpid": "Minczuk-M" } ], "contributor": [ { "family_name": "Liu", "given_name": "Jia", "clpid": "Liu-Jia" } ], "abstract": "Mitochondrial diseases often result from mutations in the mitochondrial genome (mtDNA). In most cases, mutant mtDNA coexists with wild-type mtDNA, resulting in heteroplasmy. One potential future approach to treat heteroplasmic mtDNA diseases is the specific elimination of pathogenic mtDNA mutations, lowering the level of mutant mtDNA below pathogenic thresholds. Mitochondrially targeted zinc-finger nucleases (mtZFNs) have been demonstrated to specifically target and introduce double-strand breaks in mutant mtDNA, facilitating substantial shifts in heteroplasmy. One application of mtZFN technology, in the context of heteroplasmic mtDNA disease, is delivery into the heteroplasmic oocyte or early embryo to eliminate mutant mtDNA, preventing transmission of mitochondrial diseases through the germline. Here we describe a protocol for efficient production of mtZFN mRNA in vitro, and delivery of these into 0.5 dpc mouse embryos to elicit shifts of mtDNA heteroplasmy.", "doi": "10.1007/978-1-4939-8799-3_16", "isbn": "9781493987986", "publisher": "Humana Press", "place_of_publication": "New York, NY", "publication_date": "2018-08-29", "pages": "215-228" }, { "id": "authors:yzw1m-ktr05", "collection": "authors", "collection_id": "yzw1m-ktr05", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190409-140137785", "type": "book_section", "title": "Polarity in Cell-Fate Acquisition in the Early Mouse Embryo", "book_title": "Mammalian Preimplantation Development", "author": [ { "family_name": "Leung", "given_name": "C. Y.", "clpid": "Leung-Chuen-Yan" }, { "family_name": "Zhu", "given_name": "M.", "orcid": "0000-0001-6157-8840", "clpid": "Zhu-Meng" }, { "family_name": "Zernicka-Goetz", "given_name": "M.", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" } ], "contributor": [ { "family_name": "DePamphilis", "given_name": "Melvin L.", "clpid": "DePamphilis-M-L" } ], "abstract": "Establishing polarity is a fundamental part of embryogenesis and can be traced back to the earliest developmental stages. It can be achieved in one of two ways: through the preexisting polarization of germ cells before fertilization or via symmetry breaking after fertilization. In mammals, it seems to be the latter, and we will discuss the various cytological and molecular events that lead up to this event, its mechanisms and the consequences. In mammals, the first polarization event occurs in the preimplantation period, when the embryo is but a cluster of cells, free-floating in the oviduct. This provides a unique, autonomous system to study the de novo polarization that is essential to life. In this review, we will cover modern and past studies on the polarization of the early embryo, using the mouse as a model system, as well as hypothesizing the potential implications and functions of the biological events involved.", "doi": "10.1016/bs.ctdb.2016.04.008", "isbn": "9780128014288", "publisher": "Elsevier", "place_of_publication": "New York, NY", "publication_date": "2016-06-07", "pages": "203-234" }, { "id": "authors:m0xxa-qfp83", "collection": "authors", "collection_id": "m0xxa-qfp83", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190405-170315260", "type": "book_section", "title": "The Acquisition of Cell Fate in Mouse Development: How Do Cells First Become Heterogeneous?", "book_title": "Essays on Developmental Biology, Part B", "author": [ { "family_name": "Graham", "given_name": "Sarah J. L.", "clpid": "Graham-S-J-L" }, { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" } ], "contributor": [ { "family_name": "Wassarman", "given_name": "Paul M.", "clpid": "Wassarman-P-M" } ], "abstract": "Specification of cell fate in the early mouse embryo involves the generation of heterogeneities between cells in polarity, position, gene expression, and protein activity. Identifying when and how these differences first become established represents an important challenge for understanding the roles of stochastic and cell history-dependent mechanisms in mammalian development.", "doi": "10.1016/bs.ctdb.2015.11.021", "isbn": "9780128013823", "publisher": "Elsevier", "place_of_publication": "New York, NY", "publication_date": "2016-01-21", "pages": "671-695" }, { "id": "authors:a03mj-0y409", "collection": "authors", "collection_id": "a03mj-0y409", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190417-152710198", "type": "book_section", "title": "Formation of Distinct Cell Types in the Mouse Blastocyst", "book_title": "Mouse Development: From Oocyte to Stem Cells", "author": [ { "family_name": "Morris", "given_name": "Samantha A.", "clpid": "Morris-S-A" }, { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" } ], "contributor": [ { "family_name": "Kubiak", "given_name": "Jacek Z.", "clpid": "Kubiak-J-Z" } ], "abstract": "Early development of the mouse comprises a sequence of cell fate decisions in which cells are guided along a pathway of restricted potential and increasing specialisation. The first choice faced by cells of the embryo is whether to become trophectoderm (TE) or inner cell mass (ICM); TE is an extra-embryonic tissue which will form the embryonic portion of the placenta, whilst ICM gives rise to cells responsible for generating the foetus. In the second cell fate decision, the ICM is further refined into pluripotent cells forming the future body of the embryo, epiblast (EPI) and extra-embryonic primitive endoderm (PE), a tissue essential for patterning the embryo and establishing the developmental circulation. Understanding this early lineage segregation is critical for informing attempts to capture pluripotency and direct cell fate in vitro. Unlike the predictability of nonmammalian cell fate, development of the mouse embryo retains the flexibility to adapt to changing circumstances during development. Here we describe these first cell fate decisions, how they can be biased whilst maintaining flexibility and, finally, some of the molecular circuitry underlying early fate choice.", "doi": "10.1007/978-3-642-30406-4_11", "isbn": "9783642304057", "publisher": "Springer Berlin Heidelberg", "place_of_publication": "Berlin, Heidelberg", "publication_date": "2012-07-21", "pages": "203-217" }, { "id": "authors:8f3rk-h7p71", "collection": "authors", "collection_id": "8f3rk-h7p71", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736360", "type": "book_section", "title": "Cell Lineage Analysis: Applications of Green Fluorescent Protein", "book_title": "Developmental Biology Protocols", "author": [ { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" }, { "family_name": "Pines", "given_name": "Jonathon", "orcid": "0000-0002-5227-6004", "clpid": "Pines-J" } ], "contributor": [ { "family_name": "Walker", "given_name": "John M.", "clpid": "Walker-J-M" }, { "family_name": "Tuan", "given_name": "Rocky S.", "clpid": "Tuan-Rocky-S" }, { "family_name": "Lo", "given_name": "Cecilia W.", "clpid": "Lo-Cecilia-W" } ], "abstract": "The ideal cell lineage marker is one that can be visualized in living tissues without perturbing development. Exogenously applied dyes are very useful, but in many instances there would be advantages to an endogenously expressed marker. Green fluorescent protein (GFP) appears to have the potential to be ideal for this purpose, and with its advent, lineage analysis is entering a new phase in which cells can now be followed in real time in living embryos. GFP is a 27-kDa protein found in the jellyfish Aequorea victoria that absorbs blue light and emits green light. It has the valuable property that the formation of the chromophore is an autocatalytic event that requires no cofactors. Furthermore, the cDNA that encodes GFP has been cloned, and thus GFP can be expressed and will become fluorescent in any organism or cell type. In this chapter, we describe the properties of GFP and the different mutants available to study cell lineages in different organisms. We will mainly concentrate on their application to the analysis of cell lineages in frogs and mice.", "doi": "10.1385/1-59259-685-1:279", "isbn": "1592596851", "publisher": "Humana Press", "place_of_publication": "Totowa, NJ", "publication_date": "2003-11-15", "pages": "279-287" }, { "id": "authors:71a38-eqz76", "collection": "authors", "collection_id": "71a38-eqz76", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408600", "type": "book_section", "title": "RNA Interference in Mammalian Systems - A Practical Approach", "book_title": "Peroxisomal Disorders and Regulation of Genes", "author": [ { "family_name": "Grabarek", "given_name": "Joanna B.", "clpid": "Grabarek-J-B" }, { "family_name": "Zernicka-Goetz", "given_name": "Magdalena", "orcid": "0000-0002-7004-2471", "clpid": "Zernicka-Goetz-M" } ], "contributor": [ { "family_name": "Roels", "given_name": "Frank", "clpid": "Roels-F" }, { "family_name": "Baes", "given_name": "Myriam", "clpid": "Baes-M" }, { "family_name": "De Bie", "given_name": "Sylvia", "clpid": "De-Bie-S" } ], "abstract": "RNAi-related mechanisms are evolutionarily conserved and are a tool for cells to defend against foreign nucleic acid invasion and thus genomic instability in the cell. Double-stranded RNA is relatively easy to produce in vitro and, its introduction generates knockout phenotypes without the need to interfere at the DNA level. Thus, the discovery of RNAi has greatly contributed to research by providing a new genetic tool. \n\nThis chapter presents the comparative view on the RNAi-releated techniques and discusses their practical applications in different mammalian experimental systems, including their therapeutic potential.", "doi": "10.1007/978-1-4419-9072-3_24", "isbn": "9781461347828", "publisher": "Springer", "place_of_publication": "Boston, MA", "publication_date": "2003", "pages": "205-216" } ]