[ { "id": "authors:zsx8s-v8510", "collection": "authors", "collection_id": "zsx8s-v8510", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200515-090423680", "type": "article", "title": "Cell\u2010extracellular matrix interactions in the fluidic phase direct the topology and polarity of self\u2010organized epithelial structures", "author": [ { "family_name": "Ouyang", "given_name": "Mingxing", "orcid": "0000-0002-2910-0713", "clpid": "Ouyang-Mingxing" }, { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Chen", "given_name": "Yenyu", "clpid": "Chen-Yenyu" }, { "family_name": "Deng", "given_name": "Linhong", "orcid": "0000-0003-1970-8239", "clpid": "Deng-Linhong" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "Introduction: In vivo, cells are surrounded by extracellular matrix (ECM). To build organs from single cells, it is generally believed that ECM serves as scaffolds to coordinate cell positioning and differentiation. Nevertheless, how cells utilize cell\u2010ECM interactions for the spatiotemporal coordination to different ECM at the tissue scale is not fully understood. Methods: Here, using in vitro assay with engineered MDCK cells expressing H2B\u2010mCherry (nucleus) and gp135/Podocalyxin\u2010GFP (apical marker), we show in multi\u2010dimensions that such coordination for epithelial morphogenesis can be determined by cell\u2010soluble ECM interaction in the fluidic phase. Results: The coordination depends on the native topology of ECM components such as sheet\u2010like basement membrane (BM) and type I collagen (COL) fibres: scaffold formed by BM (COL) facilitates a close\u2010ended (open\u2010ended) coordination that leads to the formation of lobular (tubular) epithelium. Further, cells form apicobasal polarity throughout the entire lobule/tubule without a complete coverage of ECM at the basal side, and time\u2010lapse two\u2010photon scanning imaging reveals the polarization occurring early and maintained through the lobular expansion. During polarization, gp135\u2010GFP was converged to the apical surface collectively in the lobular/tubular structures, suggesting possible intercellular communications. Under suspension culture, the polarization was impaired with multi\u2010lumen formation in the tubules, implying the importance of ECM biomechanical microenvironment. Conclusion: Our results suggest a biophysical mechanism for cells to form polarity and coordinate positioning at tissue scale, and in engineering epithelium through cell\u2010soluble ECM interaction and self\u2010assembly.", "doi": "10.1111/cpr.13014", "issn": "0960-7722", "publisher": "Wiley", "publication": "Cell Proliferation", "publication_date": "2021-04", "series_number": "4", "volume": "54", "issue": "4", "pages": "Art. No. e13014" }, { "id": "authors:w2gyg-kav35", "collection": "authors", "collection_id": "w2gyg-kav35", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20181114-153057569", "type": "article", "title": "Multiphoton structured thin-plane imaging with a single optical path", "author": [ { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Kim", "given_name": "Jonghyun", "orcid": "0000-0002-2198-2520", "clpid": "Kim-Jonghyun" }, { "family_name": "Holland", "given_name": "Daniel B.", "clpid": "Holland-D-B" }, { "family_name": "Mou", "given_name": "Yun", "clpid": "Mou-Yun" }, { "family_name": "Chiu", "given_name": "Hui", "clpid": "Chiu-Hui" }, { "family_name": "Blake", "given_name": "Geoffrey A.", "orcid": "0000-0003-0787-1610", "clpid": "Blake-G-A" }, { "family_name": "Lee", "given_name": "Wonhee", "clpid": "Lee-Wonhee" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "Optical sectioning has become an indispensable technique for high-speed volumetric imaging in the past decade. Here we present a novel optical-sectioning method that produces a thin plane of illumination by exploiting the spatial and temporal properties of multiphoton excitation. Critically, the illumination and detection share the same optical path, as in a conventional epi-fluorescence microscope configuration. Therefore, the imaged sample can be prepared as for standard fluorescence microscopy. Our method also leads to a laterally structured illumination pattern, and this feature can be utilized in structured illumination microscopy to further enhance the imaging performance. We show an example of such an approach, which achieves axial resolution finer than confocal microscopy. We also demonstrate the potential of the new method for biological applications by performing three-dimensional imaging of living Caenorhabditis elegans.", "doi": "10.1364/ol.43.005271", "issn": "0146-9592", "publisher": "Optical Society of America", "publication": "Optics Letters", "publication_date": "2018-11-01", "series_number": "21", "volume": "43", "issue": "21", "pages": "5271-5274" }, { "id": "authors:kxkdz-6ew15", "collection": "authors", "collection_id": "kxkdz-6ew15", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20181011-115949691", "type": "article", "title": "Fiber-bundle illumination: realizing high-degree time-multiplexed multifocal multiphoton microscopy with simplicity", "author": [ { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Kim", "given_name": "Sunduck", "clpid": "Kim-Sunduck" }, { "family_name": "Shim", "given_name": "Young Bo", "clpid": "Shim-Young-Bo" }, { "family_name": "Holland", "given_name": "Daniel B.", "clpid": "Holland-D-B" }, { "family_name": "Allodi", "given_name": "Marco A.", "orcid": "0000-0002-3289-1659", "clpid": "Allodi-M-A" }, { "family_name": "Yeh", "given_name": "Chao-Yuan", "clpid": "Yeh-Chao-Yuan" }, { "family_name": "Blake", "given_name": "Geoffrey A.", "orcid": "0000-0003-0787-1610", "clpid": "Blake-G-A" }, { "family_name": "Han", "given_name": "Young-Geun", "clpid": "Han-Young-Geun" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "High-degree time-multiplexed multifocal multiphoton microscopy was expected to provide a facile path to scanningless optical-sectioning and the fast imaging of dynamic three-dimensional biological systems. However, physical constraints on typical time multiplexing devices, arising from diffraction in the free-space propagation of light waves, lead to significant manufacturing difficulties and have prevented the experimental realization of high-degree time multiplexing. To resolve this issue, we have developed a novel method using optical fiber bundles of various lengths to confine the diffraction of propagating light waves and to create a time multiplexing effect. Through this method, we experimentally demonstrate the highest degree of time multiplexing ever achieved in multifocal multiphoton microscopy (~50 times larger than conventional approaches), and hence the potential of using simply-manufactured devices for scanningless optical sectioning of biological systems.", "doi": "10.1038/s41598-018-33286-1", "pmcid": "PMC6173774", "issn": "2045-2322", "publisher": "Nature Publishing Group", "publication": "Scientific Reports", "publication_date": "2018-10-05", "volume": "8", "pages": "Art. No. 14863" }, { "id": "authors:xzma1-83664", "collection": "authors", "collection_id": "xzma1-83664", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150528-195712684", "type": "article", "title": "Computational design of co-assembling protein\u2013DNA nanowires", "author": [ { "family_name": "Mou", "given_name": "Yun", "clpid": "Mou-Yun" }, { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Wannier", "given_name": "Timothy M.", "clpid": "Wannier-T-M" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" }, { "family_name": "Mayo", "given_name": "Stephen L.", "orcid": "0000-0002-9785-5018", "clpid": "Mayo-S-L" } ], "abstract": "Biomolecular self-assemblies are of great interest to nanotechnologists because of their functional versatility and their biocompatibility. Over the past decade, sophisticated single-component nanostructures composed exclusively of nucleic acids, peptides and proteins have been reported, and these nanostructures have been used in a wide range of applications, from drug delivery to molecular computing. Despite these successes, the development of hybrid co-assemblies of nucleic acids and proteins has remained elusive. Here we use computational protein design to create a protein\u2013DNA co-assembling nanomaterial whose assembly is driven via non-covalent interactions. To achieve this, a homodimerization interface is engineered onto the Drosophila Engrailed homeodomain (ENH), allowing the dimerized protein complex to bind to two double-stranded DNA (dsDNA) molecules. By varying the arrangement of protein-binding sites on the dsDNA, an irregular bulk nanoparticle or a nanowire with single-molecule width can be spontaneously formed by mixing the protein and dsDNA building blocks. We characterize the protein\u2013DNA nanowire using fluorescence microscopy, atomic force microscopy and X-ray crystallography, confirming that the nanowire is formed via the proposed mechanism. This work lays the foundation for the development of new classes of protein\u2013DNA hybrid materials. Further applications can be explored by incorporating DNA origami, DNA aptamers and/or peptide epitopes into the protein\u2013DNA framework presented here.", "doi": "10.1038/nature14874", "issn": "0028-0836", "publisher": "Nature Publishing Group", "publication": "Nature", "publication_date": "2015-09-10", "series_number": "7568", "volume": "525", "issue": "7568", "pages": "230-233" }, { "id": "authors:9gwz2-pmn63", "collection": "authors", "collection_id": "9gwz2-pmn63", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130424-100055385", "type": "article", "title": "The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy", "author": [ { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Holland", "given_name": "Daniel B.", "clpid": "Holland-D-B" }, { "family_name": "Blake", "given_name": "Geoffrey A.", "orcid": "0000-0003-0787-1610", "clpid": "Blake-G-A" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "We present a theoretical investigation of an optical microscope design that achieves wide-field, multiphoton fluorescence microscopy with finer axial resolution than confocal microscopy. Our technique creates a thin plane of excitation light at the sample using height-staggered microlens arrays (HSMAs), wherein the height staggering of microlenses generate temporal focusing to suppress out-of-focus excitation, and the dense spacing of microlenses enables the implementation of structured illumination technique to eliminate residual out-of-focus signal. We use physical optics-based numerical simulations to demonstrate that our proposed technique can achieve diffraction-limited three-dimensional imaging through a simple optical design.", "doi": "10.1364/OE.21.002097", "issn": "1094-4087", "publisher": "Optical Society of America", "publication": "Optics Express", "publication_date": "2013-01-28", "series_number": "2", "volume": "21", "issue": "2", "pages": "2097-2109" }, { "id": "authors:fts5t-h6h79", "collection": "authors", "collection_id": "fts5t-h6h79", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200519-144050973", "type": "book_section", "title": "Long-Range Mechanical Force Enables Self-Assembly of Epithelial Tubules", "book_title": "Mechanics of Biological Systems and Materials", "author": [ { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" }, { "family_name": "Ouyang", "given_name": "Mingxing", "clpid": "Ouyang-Mingxing" }, { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" } ], "contributor": [ { "family_name": "Prorok", "given_name": "Barton C.", "clpid": "Prorok-B-C" }, { "family_name": "Barthelat", "given_name": "Fran\u00e7ois", "clpid": "Barthelat-F" }, { "family_name": "Korach", "given_name": "Chad S.", "clpid": "Korach-C-S" }, { "family_name": "Grande-Allen", "given_name": "K. Jane", "clpid": "Grande-Allen-K-J" }, { "family_name": "Lipke", "given_name": "Elizabeth", "clpid": "Lipke-E" }, { "family_name": "Lykofatitits", "given_name": "George", "clpid": "Lykofatitits-G" }, { "family_name": "Zavattieri", "given_name": "Pablo", "clpid": "Zavattieri-P" } ], "abstract": "Spatiotemporal coordination of cell positioning and differentiation is critical in morphogenesis. Loss of coordination is often a hallmark of tissue abnormality and tumorigenesis. Recent studies indicate the importance of mechanical force in morphogenesis such as tubular pattern formation. However, how cells coordinate mechanical interactions between each other and with extracellular matrix (ECM), to initiate, regulate, or maintain long-range tubular patterns is unclear. Using a two-step process to quantitatively control cell-ECM interaction, we find that epithelial cells, in response to a fine-tuned percentage of type I collagen (COL) in ECM, develop various patterns resembling those observed in tubulo-lobular organs. In contrast with conventional thought, these patterns arise through mechanical interactions between cells, but not through gradients of diffusible biochemical factors. Remarkably, a very large spatial scale of tubular patterns is found by cell-COL self-organization in the liquid phase, leading to the formation of long-range (~1 cm) epithelial tubule. Our results suggest a potential mechanism cells can use to form and coordinate long-range tubular patterns, independent of those controlled by diffusible biochemical factors, and provide a new strategy to engineer/regenerate tubular organs.", "doi": "10.1007/978-1-4614-4427-5_3", "isbn": "978-1-4614-4426-8", "publisher": "Springer", "place_of_publication": "New York, NY", "publication_date": "2012-09-10", "pages": "15-21" }, { "id": "authors:rc7et-8ts36", "collection": "authors", "collection_id": "rc7et-8ts36", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120525-095248642", "type": "article", "title": "Long-range mechanical force enables self-assembly of epithelial tubular patterns", "author": [ { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" }, { "family_name": "Ouyang", "given_name": "Mingxing", "clpid": "Ouyang-Mingxing" }, { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Maslov", "given_name": "Jordan", "clpid": "Maslov-Jordan" }, { "family_name": "Price", "given_name": "Andrew", "clpid": "Price-Andrew" }, { "family_name": "Shen", "given_name": "Chih-Yu", "clpid": "Shen-Chih-Yu" } ], "abstract": "Enabling long-range transport of molecules, tubules are critical for human body homeostasis. One fundamental question in tubule formation is how individual cells coordinate their positioning over long spatial scales, which can be as long as the sizes of tubular organs. Recent studies indicate that type I collagen (COL) is important in the development of epithelial tubules. Nevertheless, how cell\u2013COL interactions contribute to the initiation or the maintenance of long-scale tubular patterns is unclear. Using a two-step process to quantitatively control cell\u2013COL interaction, we show that epithelial cells developed various patterns in response to fine-tuned percentages of COL in ECM. In contrast with conventional thoughts, these patterns were initiated and maintained by traction forces created by cells but not diffusive factors secreted by cells. In particular, COL-dependent transmission of force in the ECM led to long-scale (up to 600 \u03bcm) interactions between cells. A mechanical feedback effect was encountered when cells used forces to modify cell positioning and COL distribution and orientations. Such feedback led to a bistability in the formation of linear, tubule-like patterns. Using micro-patterning technique, we further show that the stability of tubule-like patterns depended on the lengths of tubules. Our results suggest a mechanical mechanism that cells can use to initiate and maintain long-scale tubular patterns.", "doi": "10.1073/pnas.1114781109", "pmcid": "PMC3326479", "issn": "0027-8424", "publisher": "National Academy of Sciences", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "publication_date": "2012-04-10", "series_number": "15", "volume": "109", "issue": "15", "pages": "5576-5582" }, { "id": "authors:57j0r-q3064", "collection": "authors", "collection_id": "57j0r-q3064", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120106-140226269", "type": "article", "title": "Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy", "author": [ { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Kuo", "given_name": "Chun-Hung", "clpid": "Kuo-Chun-Hung" }, { "family_name": "Holland", "given_name": "Daniel B.", "clpid": "Holland-D-B" }, { "family_name": "Chen", "given_name": "Yenyu", "clpid": "Chen-Yenyu" }, { "family_name": "Ouyang", "given_name": "Mingxing", "clpid": "Ouyang-Mingxing" }, { "family_name": "Blake", "given_name": "Geoffrey A.", "orcid": "0000-0003-0787-1610", "clpid": "Blake-G-A" }, { "family_name": "Zadoyan", "given_name": "Ruben", "clpid": "Zadoyan-R" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "Optical sectioning provides three-dimensional (3D) information in biological tissues. However, most imaging techniques implemented with optical sectioning are either slow or deleterious to live tissues. Here, we present a simple design for wide-field multiphoton microscopy, which provides optical sectioning at a reasonable frame rate and with a biocompatible laser dosage. The underlying mechanism of optical sectioning is diffuser-based temporal focusing. Axial resolution comparable to confocal microscopy is theoretically derived and experimentally demonstrated. To achieve a reasonable frame rate without increasing the laser power, a low-repetition-rate ultrafast laser amplifier was used in our setup. A frame rate comparable to that of epifluorescence microscopy was demonstrated in the 3D imaging of fluorescent protein expressed in live epithelial cell clusters. In this report, our design displays the potential to be widely used for video-rate live-tissue and embryo imaging with axial resolution comparable to laser scanning microscopy.", "doi": "10.1117/1.3647570", "issn": "1083-3668", "publisher": "Society of Photo-Optical Instrumentation Engineers", "publication": "Journal of Biomedical Optics", "publication_date": "2011-11", "series_number": "11", "volume": "16", "issue": "11", "pages": "Art. No. 116009" }, { "id": "authors:c6375-16n37", "collection": "authors", "collection_id": "c6375-16n37", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111107-141527848", "type": "book_section", "title": "Long-range mechanical force in colony branching and tumor invasion", "book_title": "Biosensing and Nanomedicine IV", "author": [ { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" }, { "family_name": "Ouyang", "given_name": "Mingxing", "clpid": "Ouyang-Mingxing" }, { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Price", "given_name": "Andrew", "clpid": "Price-Andrew" }, { "family_name": "Maslov", "given_name": "Jordan", "clpid": "Maslov-Jordan" } ], "contributor": [ { "family_name": "Mohseni", "given_name": "Hooman", "clpid": "Mohseni-H" }, { "family_name": "Agahi", "given_name": "Massoud H.", "clpid": "Agahi-M-H" }, { "family_name": "Razeghi", "given_name": "Manijeh", "clpid": "Razeghi-M" } ], "abstract": "The most concerned factors for cancer prognosis are tumor invasion and metastasis. The patterns of tumor invasion can be characterized as random infiltration to surrounding extracellular matrix (ECM) or formation of long-range path for collective migration. Recent studies indicate that mechanical force plays an important role in tumor infiltration and collective migration. However, how tumor colonies develop mechanical interactions with each other to initiate various invasion patterns is unclear. Using a micro-patterning technique, we partition cells into clusters to mimic tumor colonies and quantitatively induce colony-ECM interactions. We find that pre-malignant epithelial cells, in response to concentrations of type I collagen in ECM ([COL]), develop various branching patterns resembling those observed in tumor invasion. In contrast with conventional thought, these patterns require long-range (~ 600 \u03bcm) transmission of traction force, but not biochemical factors. At low [COL], cell colonies synergistically develop pairwise and directed branching mimicking the formation of long-range path. By contrast, at high [COL] or high colony density, cell colonies develop random branching and scattering patterns independent of each other. Our results suggest that tumor colonies might select different invasive patterns depending on their interactions with each other and with the ECM.", "doi": "10.1117/12.911904", "isbn": "9780819487094", "publisher": "SPIE", "place_of_publication": "Bellingham, WA", "publication_date": "2011-09-12", "pages": "Art. No. 809903" }, { "id": "authors:9ja8v-e1d09", "collection": "authors", "collection_id": "9ja8v-e1d09", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120330-155304712", "type": "book_section", "title": "Plane-projection multi-photon microscopy for high-frame-rate Live Tissue Imaging", "book_title": "2011 Conference on Lasers and Electro-Optics (CLEO)", "author": [ { "family_name": "Yu", "given_name": "Jiun-Yann", "clpid": "Yu-Jiun-Yann" }, { "family_name": "Kuo", "given_name": "Chun-Hung", "clpid": "Kuo-Chun-Hung" }, { "family_name": "Zadoyan", "given_name": "Ruben", "clpid": "Zadoyan-Ruben" }, { "family_name": "Guo", "given_name": "Chin-Lin", "clpid": "Guo-Chin-Lin" } ], "abstract": "We present a wide-field multi-photon microscopy that provides optical sectioning at high frame rate under biocompatible laser dosage. Axial resolution comparable to confocal microscopy and 5-frame-per-second live tissue imaging are demonstrated.", "isbn": "978-1-4577-1223-4", "publisher": "Optical Society of America", "place_of_publication": "Washington, DC", "publication_date": "2011-05", "pages": "PDPB4" } ]