@conference_item {CaltechAUTHORS_https://authors.library.caltech.edu/id/eprint/107235,
    title ="Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma",
    author = "Du, Jiajun and Su, Yapeng",
    
    
    
    pages = "PHYS-0320",
    month = "August",
    year = "2020",
    
    
    
    url = "https://resolver.caltech.edu/CaltechAUTHORS:20201221-101511711",
    note = "© 2020 American Chemical Society.",
    revision_no = "9",
    abstract = "We utilized Raman spectro-microscopy to non-invasively probe metabomics within single live cells, aiming to identify\ndruggable metabolic susceptibilities from a series of patient-derived BRAF mutant melanoma cell lines. Each cell line\nrepresents a phenotype with different characteristic level of de-differentiation and BRAFi (BRAF inhibitor) resistance.\nFirst, with single-cell Raman spectroscopy and stimulated Raman scattering (SRS) microscopy, followed by\ntranscriptomics anal., we identified lipid processes as major metabolic functional difference between different\nphenotypes. We then utilized hyperspectral-SRS imaging on intracellular single organelles to identify a previously\nunknown susceptibility of lipid desatn. within de-differentiated cell lines. Drugging this target leads to cellular apoptosis\naccompanied by phase sepd. intracellular domains. The integration of subcellular Raman spectro-microscopy with\nlipidomics and transcriptomics suggests highly heterogenous metabolic responses and possible lipid regulatory\nmechanisms underlying this pharmacol. treatment. Our method should provide a general approach in spatially-resolved\nsingle cell metabolomics studies.",
}
@conference_item {CaltechAUTHORS_https://authors.library.caltech.edu/id/eprint/97792,
    title ="Stimulated Raman imaging with chemical probes for subcellular bioanalysis",
    author = "Wei, Lu",
    
    
    
    pages = "ANYL-0316",
    month = "August",
    year = "2019",
    
    
    
    url = "https://resolver.caltech.edu/CaltechAUTHORS:20190812-141405591",
    note = "© 2019 American Chemical Society.",
    revision_no = "11",
    abstract = "Innovations in optical spectroscopy and microscopy have revolutionized our understanding in biol. systems at subcellular levels. In this talk, I will present recent advances of two chem. imaging strategies addressing two fundamental challenges in optical bio-imaging. First, we devised a live-cell Bioorthogonal Chem. Imaging platform suited for probing the metabolic dynamics of small bio-mols., which cannot be effectively labeled by bulky fluorophores. This scheme couples the stimulated Raman scattering (SRS) microscopy, a nonlinear vibrational imaging modality that offers rich chem. information, with small vibrational tags including stable isotopes and triple bonds. We applied this platform to a variety of dynamical biol. systems and revealed previously less-known heterogeneity in metabolic signatures governing the obversed cell phenotypes. Second, we developed a supermultiplex optical imaging technique enabled by preresonant SRS microscopy of a newly designed palette of vibrational dyes. This work allows for simultaneous imaging of a large no. of mol. species in live cells and tissues with high sensitivity and specificity, bridging imaging and omics paradigms. Both phys. and chem. principles underlying the optical microscopy will be presented, as well as our recent efforts in biomedical applications including cancer- and neuronal- metab.",
}