[ { "id": "https://authors.library.caltech.edu/records/8gy2d-03z19", "eprint_id": 76109, "eprint_status": "archive", "datestamp": "2023-08-20 06:26:27", "lastmod": "2023-10-25 15:31:34", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lee-J-C", "name": { "family": "Lee", "given": "J. C." } }, { "id": "VijayRaghavan-K", "name": { "family": "VijayRaghavan", "given": "K." } }, { "id": "Celniker-S-E", "name": { "family": "Celniker", "given": "S. E." } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "M. A." } } ] }, "title": "Identification of a Drosophila muscle development gene with structural homology to mammalian early growth response transcription factors", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1995 National Academy of Sciences. \n\nCommunicated by Gunther S. Stent, University of California, Berkeley, CA, June 27, 1995 (received for review May 2, 1995) \n\nWe thank A. Kolodkin for stock sr^(B14.0) and genomic DNA clones; C. Oh, E. Reynolds, and P. Pavlidis for comments on the manuscript and technical advice; M. Palazzolo for technical advice; S. Faulhaber, A. Francisco, D. Chang, and R. Young for technical assistance; and S. Benzer for use of laboratory facilities. This work was supported in part by Public Health Service National Research Service Award GM15867-02 to J.C.L. and by grants from the Department of Biotechnology, Government of India, and the Rockefeller Foundation to KV. \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.\n\n
Published - 10344.full.pdf
", "abstract": "In Drosophila, stripe (sr) gene function is required for normal muscle development. Some mutations disrupt embryonic muscle development and are lethal. Other mutations cause total loss of only a single muscle in the adult. Molecular analysis shows that sr encodes a predicted protein containing a zinc finger motif. This motif is homologous to the DNA binding domains encoded by members of the early growth response (egr) gene family. In mammals, expression of egr genes is induced by intercellular signals, and there is evidence for their role in many developmental events. The identification of sr as an egr gene and its pattern of expression suggest that it functions in muscle development via intercellular communication.", "date": "1995-10-24", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "92", "number": "22", "publisher": "National Academy of Sciences", "pagerange": "10344-10348", "id_number": "CaltechAUTHORS:20170408-160016095", "issn": "1091-6490", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170408-160016095", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH Predoctoral Fellowship", "grant_number": "GM15867-02" }, { "agency": "Department of Biotechnology (India)" }, { "agency": "Rockefeller Foundation" } ] }, "doi": "10.1073/pnas.92.22.10344", "pmcid": "PMC40793", "primary_object": { "basename": "10344.full.pdf", "url": "https://authors.library.caltech.edu/records/8gy2d-03z19/files/10344.full.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Lee, J. C.; VijayRaghavan, K.; et el." }, { "id": "https://authors.library.caltech.edu/records/e7ab1-ypy93", "eprint_id": 8918, "eprint_status": "archive", "datestamp": "2023-08-22 06:53:54", "lastmod": "2023-10-23 17:23:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Leonard-R-J", "name": { "family": "Leonard", "given": "R. J." } }, { "id": "Karschin-A", "name": { "family": "Karschin", "given": "A." } }, { "id": "Jayashree-Aiyar-S", "name": { "family": "Jayashree-Aiyar", "given": "S." } }, { "id": "Davidson-N", "name": { "family": "Davidson", "given": "N." } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "M. A." } }, { "id": "Thomas-L", "name": { "family": "Thomas", "given": "L." } }, { "id": "Thomas-G", "name": { "family": "Thomas", "given": "G." } }, { "id": "Lester-H-A", "name": { "family": "Lester", "given": "H. A." }, "orcid": "0000-0002-5470-5255" } ] }, "title": "Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus", "ispublished": "pub", "full_text_status": "public", "keywords": "heterologous expression; rat basophilic leukemia cells; rat pheochromocytoma cells", "note": "\u00a9 1989 by the National Academy of Sciences. \n\nContributed by N. Davidson, July 14, 1989. \n\nWe thank Barbara Thorne for expert assistance in the construction of the recombinant virus and J.H. Strauss and R.W. Aldrich for advice. Charybdotoxin was a generous gift of Dr. M.L. Garcia (Merck Sharp & Dohme Research Laboratories). This work was supported by National Institutes of Health Grants GM10991, GM29836, and DK37274, by the Cystic Fibrosis Foundation, and by fellowships from the Max Kade Foundation (A.K.) and the National Institutes of Health (R.J.L.). \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.\n\nPublished - LEOpnas89.pdf
", "abstract": "A recombinant vaccinia virus containing a Drosophila potassium channel (Shaker H4) cDNA was constructed by homologous recombination between wild-type vaccinia virus DNA and a transfer plasmid. The new virus was used to infect four types of mammalian cells in culture. Electrophysiological recording 24-72 hr after infection revealed the expression of voltage-gated transient potassium channels in all four cell types. The properties of the induced currents were identical to those previously observed following injection of the Shaker H4 transcript into oocytes. Vaccinia promises to be an effective vehicle for the heterologous expression of transmembrane ion channels in a variety of cell types.", "date": "1989-10-01", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "86", "number": "19", "publisher": "National Academy of Sciences", "pagerange": "7629-7633", "id_number": "CaltechAUTHORS:LEOpnas89", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:LEOpnas89", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "GM10991" }, { "agency": "NIH", "grant_number": "GM29836" }, { "agency": "NIH", "grant_number": "DK37274" }, { "agency": "Cystic Fibrosis Foundation" }, { "agency": "Max Kade Foundation" }, { "agency": "NIH Predoctoral Fellowship" } ] }, "pmcid": "PMC298120", "primary_object": { "basename": "LEOpnas89.pdf", "url": "https://authors.library.caltech.edu/records/e7ab1-ypy93/files/LEOpnas89.pdf" }, "resource_type": "article", "pub_year": "1989", "author_list": "Leonard, R. J.; Karschin, A.; et el." }, { "id": "https://authors.library.caltech.edu/records/8f9x0-qfc94", "eprint_id": 5721, "eprint_status": "archive", "datestamp": "2023-08-22 06:18:51", "lastmod": "2023-10-16 19:22:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ramaswami-Mani", "name": { "family": "Ramaswami", "given": "Mani" } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } } ] }, "title": "Two Sodium-Channel Genes in Drosophila: Implications for Channel Diversity", "ispublished": "pub", "full_text_status": "public", "keywords": "ion channel; neurogenetics; evolution", "note": "\u00a9 1989 by the National Academy of Sciences \n\nCommunicated by Seymour Benzer, December 13, 1988 \n\nWe thank A. Goldin, V. Auld, N. Davidson, and R. Dunn for the rat Na+-channel clones that were used as hybridization probes in our experiments. We are grateful to K. Loughney and B. Ganetzky for making their unpublished results available to us. We thank U. Banedjee, M. Gautam, J. Campanelli, L. Iverson, A. Kamb, A. Lashgari, M. Mathew, K. McCormack, J. Robinson, B. Rudy, and W.W. Trevarrow for helpful discussions throughout the course of the work. We also thank R. McMahon for excellent technical assistance. This research was supported by the Pfeiffer Research Foundation, and by U.S. Public Health Service Grant NS21327-01 (M.A.T.). M.R. was supported by fellowships from the Evelyn Sharp Foundation and the Markey Charitable Trust. M.A.T. is a McKnight Foundation Scholar and a Sloan Foundation Fellow. \n\nThe sequence discussed in this paper is being deposited in the EMBL/GenBank data base (accession no. J04508). \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.\n\nPublished - RAMpnas89.pdf
", "abstract": "We describe two Drosophila melanogaster transcription units that are highly homologous to a rat Na+-channel cDNA. They appear to encode the major subunits of two distinct Na+-channel proteins. One of these maps to the second chromosome and is identical to a Na+-channel gene whose partial sequence has been previously reported [Salkoff, L., Butler, A., Wei, A., Scavarda, N., Giffen, K., Ifune, K., Goodman, R. & Mandel, G. (1987) Science 237, 744-749]. The other transcription unit maps to position 14C/D, on the X chromosome, close to the paralyzed (para) gene. Mutations in para affect membrane excitability in Drosophila neurons [Ganetzky, B. & Wu, C. F. (1986) Annu. Rev. Genet. 20, 13-44]. Sequence comparisons suggest that two Na+-channel genes arose early in evolution, before the divergence of vertebrate and invertebrate lines.", "date": "1989-03-15", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "86", "number": "6", "publisher": "National Academy of Sciences", "pagerange": "2079-2082", "id_number": "CaltechAUTHORS:RAMpnas89", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:RAMpnas89", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pfeiffer Research Foundation" }, { "agency": "NIH", "grant_number": "NS21327-01" }, { "agency": "Evelyn Sharp Foundation" }, { "agency": "Lucille P. Markey Charitable Trust" }, { "agency": "McKnight Foundation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1073/pnas.86.6.2079", "pmcid": "PMC286851", "primary_object": { "basename": "RAMpnas89.pdf", "url": "https://authors.library.caltech.edu/records/8f9x0-qfc94/files/RAMpnas89.pdf" }, "resource_type": "article", "pub_year": "1989", "author_list": "Ramaswami, Mani and Tanouye, Mark A." }, { "id": "https://authors.library.caltech.edu/records/kd917-8zq70", "eprint_id": 5709, "eprint_status": "archive", "datestamp": "2023-08-22 05:56:07", "lastmod": "2023-10-23 17:19:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Iverson-L-E", "name": { "family": "Iverson", "given": "L. E." } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "M. A." } }, { "id": "Lester-H-A", "name": { "family": "Lester", "given": "H. A." }, "orcid": "0000-0002-5470-5255" }, { "id": "Davidson-N", "name": { "family": "Davidson", "given": "N." } }, { "id": "Rudy-B", "name": { "family": "Rudy", "given": "B." } } ] }, "title": "A-Type Potassium Channels Expressed from Shaker Locus cDNA", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1988 by the National Academy of Sciences \n\nContributed by N. Davidson, April 1, 1988 \n\nWe thank D. Krafte for his expert advice in oocyte patch clamping, and J. Campanelli, A. Kamb, M. Mathew, J.D. Pollock, and M. Ramaswani for their comments. This research was supported by U.S. Public Health Service Grants GM26976 to B.R.; NS21327 to M.A.T., GM10991 to N.D., NS11756 to H.A.L., and a Pfeiffer Research Foundation grant to M.A.T. \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.\n\nPublished - IVEpnas88b.pdf
", "abstract": "A-type K+ currents are expressed in Xenopus oocytes injected with in vitro-synthesized transcripts from cDNAs for the Drosophila Shaker (Sh) locus. A single Sh gene product, possibly as a multimer, is sufficient for formation of functional A channels. Various Sh RNAs express A currents with distinct kinetic properties. An analysis of structure-function relationships shows that the conserved central region of Sh polypeptides determines ionic selectivity and overall channel behavior, whereas the divergent amino and carboxyl termini can modify channel kinetics. Alternative splicing of Sh gene transcripts may provide one mechanism for the generation of K+ channel diversity.", "date": "1988-08-01", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "85", "number": "15", "publisher": "National Academy of Sciences", "pagerange": "5723-5727", "id_number": "CaltechAUTHORS:IVEpnas88b", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:IVEpnas88b", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "GM26976" }, { "agency": "NIH", "grant_number": "NS21327" }, { "agency": "NIH", "grant_number": "GM10991" }, { "agency": "NIH", "grant_number": "NS11756" }, { "agency": "Pfeiffer Research Foundation" } ] }, "pmcid": "PMC281833", "primary_object": { "basename": "IVEpnas88b.pdf", "url": "https://authors.library.caltech.edu/records/kd917-8zq70/files/IVEpnas88b.pdf" }, "resource_type": "article", "pub_year": "1988", "author_list": "Iverson, L. E.; Tanouye, M. A.; et el." }, { "id": "https://authors.library.caltech.edu/records/yxjv5-qnj73", "eprint_id": 48408, "eprint_status": "archive", "datestamp": "2023-08-19 20:42:45", "lastmod": "2023-10-17 18:47:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kamb-A", "name": { "family": "Kamb", "given": "Alexander" } }, { "id": "Tseng-Crank-J", "name": { "family": "Tseng-Crank", "given": "Julie" } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } } ] }, "title": "Multiple Products of the Drosophila Shaker Gene May Contribute to Potassium Channel Diversity", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1988 Cell Press.\n\nReceived February 29, 1988; revised May 27, 1988.\n\nWe thank M. Gautam, L. Iverson, M. Mathew, M. Ramaswami, and\nB. Rudy for helpful discussions and R. McMahon for technical assistance. This research was supported by the Pfeiffer Research Foundation and by USPHS grant NS21327-01 to M. A. T. J. T.-C. was supported by NIH training grant NS07251. M. A. T. is a McKnight Foundation Scholar and a Sloan Foundation Fellow.", "abstract": "K+ channels are known through electrophysiology and pharmacology to be an exceptionally diverse group of channels. Molecular studies of the Shaker (Sh) locus in Drosophila have provided the first glimpse of K+ channel structure. The sequences of several Sh cDNA clones have been reported; none are identical. We have isolated and examined 18 additional Sh cDNAs in an attempt to understand the origin, extent, and significance of the variability. The diversity is extensive: we have already identified cDNAs representing at least nine distinct types, and Sh could potentially encode 24 or more products. This diversity, however, fits a simple pattern in which variable 3\u2032 and 5\u2032 ends are spliced onto a central constant region to yield different cDNA types. These different Sh cDNAs encode proteins with distinct structural features.", "date": "1988-07", "date_type": "published", "publication": "Neuron", "volume": "1", "number": "5", "publisher": "Elsevier", "pagerange": "421-430", "id_number": "CaltechAUTHORS:20140812-131531957", "issn": "0896-6273", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140812-131531957", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pfeiffer Research Foundation" }, { "agency": "USPHS", "grant_number": "NS21327-01" }, { "agency": "NIH", "grant_number": "NS07251" }, { "agency": "Knight Foundation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0896-6273(88)90192-4", "resource_type": "article", "pub_year": "1988", "author_list": "Kamb, Alexander; Tseng-Crank, Julie; et el." }, { "id": "https://authors.library.caltech.edu/records/2vd3a-6qg65", "eprint_id": 47391, "eprint_status": "archive", "datestamp": "2023-08-19 19:44:19", "lastmod": "2023-10-26 20:33:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kamb-A", "name": { "family": "Kamb", "given": "Alexander" } }, { "id": "Iverson-L-E", "name": { "family": "Iverson", "given": "Linda E." } }, { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } } ] }, "title": "Molecular Characterization of Shaker, a Drosophila Gene That Encodes a Potassium Channel", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1987 Cell Press.\n\nReceived June 18, 1987.\n\nA. L. and L. I. contributed equally to the work presented here. We thank D. Ballinger, U. Banerjee, M. Gautam, J. Pollock, M. Ramaswami, J. Tseng-Crank, and K. VijayRaghavan for comments. This research was supported by the Pfeiffer Research Foundation and USPHS grant NS21327-01 to M. T. L. I. was supported by a Muscular Dystrophy Association Fellowship and the E. S. Gosney Fund. A. K. was supported by NRSA training grant GM07616. M. T. is a McKnight Foundation Scholar and a Sloan Foundation Fellow. \n\nThe costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked \"advertisement\" in accordance with 18 USC. Section 1734 solely to indicate this fact.", "abstract": "The Drosophila Shaker (Sh) gene appears to encode a type of voltage-sensitive potassium (K+) channel called the A channel. We have isolated Sh as part of a 350 kb chromosomal walk. The region around Sh contains four identified transcription units. We find that Sh corresponds to a very large transcription unit encompassing a total of about 95 kb of genomic DNA and split by a major 85 kb intron. Sh has multiple hydrophobic domains that have a high probability of being membrane-spanning, consistent with the proposal that it encodes an ion channel.", "date": "1987-07-31", "date_type": "published", "publication": "Cell", "volume": "50", "number": "3", "publisher": "Elsevier", "pagerange": "405-413", "id_number": "CaltechAUTHORS:20140722-104348012", "issn": "0092-8674", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140722-104348012", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pfeiffer Research Foundation" }, { "agency": "USPHS", "grant_number": "NS21327-01" }, { "agency": "Muscular Dystrophy Association Fellowship" }, { "agency": "E. S. Gosney Fund" }, { "agency": "NIH", "grant_number": "GM07616" }, { "agency": "McKnight Foundation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0092-8674(87)90494-6", "resource_type": "article", "pub_year": "1987", "author_list": "Kamb, Alexander; Iverson, Linda E.; et el." }, { "id": "https://authors.library.caltech.edu/records/92hv9-ddg98", "eprint_id": 45752, "eprint_status": "archive", "datestamp": "2023-08-19 18:26:43", "lastmod": "2023-10-26 18:13:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } }, { "id": "Kamb-C-A", "name": { "family": "Kamb", "given": "C. A." } }, { "id": "Iverson-L-E", "name": { "family": "Iverson", "given": "Linda E." } }, { "id": "Salkoff-L", "name": { "family": "Salkoff", "given": "Lawrence" } } ] }, "title": "Genetics and Molecular Biology of Ionic Channels in Drosophila", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1986 Annual Reviews.\n\nWe thank M. Ramaswami and A. Ferrus for valuable comments and C.-F.\nWu for providing a copy of a manuscript in press. The preparation of this\nreview and some of the work presented in it was supported by the Pfeiffer\nResearch Foundation and USPHS grant NS21327-01 to M. T. L. S. was\nsupported by NSF grant BNS8311024. C. K. was supported by NRSA\ntraining grant GM07616. L. I. was supported by a Muscular Dystrophy\nAssociation Fellowship. M. T. is a McKnight Foundation Scholar and a\nSloan Foundation Fellow.", "abstract": "In this review we examine mutations that alter electrical excitability in the\nnervous system of the fruitfly, Drosophila melanogaster, and discuss how\nthese mutations may be used to approach the molecular basis of ionic\nchannel function.", "date": "1986-03", "date_type": "published", "publication": "Annual Review of Neuroscience", "volume": "9", "publisher": "Annual Reviews", "pagerange": "255-276", "id_number": "CaltechAUTHORS:20140514-153445006", "issn": "0147-006X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140514-153445006", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pfeiffer Research Foundation" }, { "agency": "USPHS", "grant_number": "NS21327-01" }, { "agency": "NSF", "grant_number": "BNS8311024" }, { "agency": "NRSA", "grant_number": "GM07616" }, { "agency": "Muscular Dystrophy Association Fellowship" }, { "agency": "McKnight Foundation Scholar Award" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1146/annurev.ne.09.030186.001351", "resource_type": "article", "pub_year": "1986", "author_list": "Tanouye, Mark A.; Kamb, C. A.; et el." }, { "id": "https://authors.library.caltech.edu/records/hf9pj-6we79", "eprint_id": 32401, "eprint_status": "archive", "datestamp": "2023-08-19 16:04:21", "lastmod": "2023-10-17 23:47:47", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } }, { "id": "King-D-G", "name": { "family": "King", "given": "David G." } } ] }, "title": "Giant Fibre Activation of Direct Flight Muscles in\n Drosophila", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1983 The Company of Biologists Limited.\nReceived 2 September 1982; Accepted 14 March 1983.\nWe thank Dr Seymour Benzer for the use of his facilities and his support during\nthese experiments. We thank our colleagues Drs L. Kauvar, S. Shotwell, B. Tempel,\nT. Venkatesh, C.-F. WuandL. Zipursky for discussion. This research was supported\nby National Science Foundation Grant PCM 7911771 to S. Benzer and the Pew\nMemorial Trust. M. A. T. was supported by National Institutes of Health Training\nGrant GM 7401-02.\n\nPublished - TANjeb83.pdf
", "abstract": "1. Electrical stimuli delivered to the brain were used to activate the giant\nfibre of Drosophila.\n2. The giant fibre drove a prominent wing opening movement.\n3. Intracellular microelectrode recordings from direct wing opener muscle\nfibres showed that giant fibre activation of an anterior pleural muscle,\npa3, was responsible for the wing opening movement.\n4. The giant fibre drove a slight wing elevation movement.\n5. Intracellular recordings from direct wing elevator muscle fibres\nshowed that these muscles were not activated by the giant fibre.\n6. It is suggested that giant fibre-driven wing elevation movements were\nmediated by the tergotrochanter muscle (TTM).", "date": "1983-07", "date_type": "published", "publication": "Journal of Experimental Biology", "volume": "105", "publisher": "Company of Biologists", "pagerange": "241-251", "id_number": "CaltechAUTHORS:20120712-163411288", "issn": "0022-0949", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120712-163411288", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF", "grant_number": "PCM 7911771" }, { "agency": "NIH Training Grant", "grant_number": "GM 7401-02" } ] }, "primary_object": { "basename": "TANjeb83.pdf", "url": "https://authors.library.caltech.edu/records/hf9pj-6we79/files/TANjeb83.pdf" }, "resource_type": "article", "pub_year": "1983", "author_list": "Tanouye, Mark A. and King, David G." }, { "id": "https://authors.library.caltech.edu/records/esx7r-p7140", "eprint_id": 10367, "eprint_status": "archive", "datestamp": "2023-08-22 02:48:48", "lastmod": "2023-10-16 22:49:37", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tanouye-M-A", "name": { "family": "Tanouye", "given": "Mark A." } }, { "id": "Ferrus-A", "name": { "family": "Ferrus", "given": "Alberto" } }, { "id": "Fujita-S-C", "name": { "family": "Fujita", "given": "Shinobu C." } } ] }, "title": "Abnormal Action Potentials Associated with the Shaker Complex Locus of Drosophila", "ispublished": "pub", "full_text_status": "public", "keywords": "behavior; neurogenetics; potassium channel; membrane physiology; gene complex", "note": "Copyright \u00a9 1981 by the National Academy of Sciences. \n\nContributed by Seymour Benzer, July 20, 1981. \n\nWe are grateful to S. Benzer for continual encouragement and support. We thank L. Kauvar, J. Reinitz, and S. Shotwell for discussion. Special thanks go to I. Duncan for cytogenetic determinations. T. Homyk, G. Lefevre, and J. Merriam kindly provided several stocks. This research was supported by National Science Foundation Grant PCM 7911771 to S. Benzer and the Pew Memorial Trust. M.A.T. was supported by a Muscular Dystrophy Association fellowship and National Institutes of Health Training Grant GM 7401-02. A.F. was supported by National Institutes of Health Fellowship F05 TWO2477 and a Gosney Foundation fellowship. \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.", "abstract": "Intracellular recordings of action potentials were made from the cervical giant axon in Shaker (Sh) mutants and normal Drosophila. The mutants showed abnormally long delays in repolarization. The defect is not due to abnormal Ca2+ channels, because it persists in the presence of Co2+, a Ca2+-channel blocker. On the other hand, the K+-channel blocker 4-aminopyridine causes a similar effect in normal animals, suggesting that the Sh mutant may have abnormal K+ conductance. Gene-dosage analysis of Sh shows that the defect is not due to underproduction of an otherwise normal molecule; it may be due to an abnormal molecule produced by the mutated gene. Gel electrophoresis failed to detect an abnormal protein, suggesting that, if Sh codes for a nervous system protein, it is rare. Genetic analysis of the Sh locus indicates three regions. Mutations or chromosome breaks in the two flanking regions cause Sh mutant physiology; the central region shows a \"haplolethal effect\"--i.e., heterozygous females are lethal.", "date": "1981-10-01", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "78", "number": "10", "publisher": "National Academy of Sciences", "pagerange": "6548-6552", "id_number": "CaltechAUTHORS:TANpnas81", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:TANpnas81", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "TANpnas81.pdf", "url": "https://authors.library.caltech.edu/records/esx7r-p7140/files/TANpnas81.pdf" }, "resource_type": "article", "pub_year": "1981", "author_list": "Tanouye, Mark A.; Ferrus, Alberto; et el." } ]