[ { "id": "authors:vx6ma-dj708", "collection": "authors", "collection_id": "vx6ma-dj708", "cite_using_url": "https://authors.library.caltech.edu/records/vx6ma-dj708", "type": "article", "title": "The inbred newt genome unveils molecular mechanisms of behavior, development, and regeneration in urodele amphibians", "author": [ { "family_name": "Kimura", "given_name": "Yuki" }, { "family_name": "Suzuki", "given_name": "Miyuki", "orcid": "0000-0002-7284-7505", "clpid": "Suzuki-Miyuki" }, { "family_name": "Okumura", "given_name": "Akinori", "orcid": "0000-0003-1080-6107" }, { "family_name": "Matsunami", "given_name": "Masatoshi", "orcid": "0000-0003-4109-2493" }, { "family_name": "Nishide", "given_name": "Hiroyo" }, { "family_name": "Mizuno", "given_name": "Rima", "orcid": "0009-0001-3237-5953" }, { "family_name": "Bou", "given_name": "Kazuto" }, { "family_name": "Uno", "given_name": "Yoshinobu" }, { "family_name": "Nakada", "given_name": "Tomoaki" }, { "family_name": "Hasunuma", "given_name": "Itaru", "orcid": "0000-0003-2785-9010" }, { "family_name": "Haramoto", "given_name": "Yoshikazu", "orcid": "0000-0002-9460-0837" }, { "family_name": "Fukui", "given_name": "Akimasa" }, { "family_name": "Inoue", "given_name": "Takeshi" }, { "family_name": "Sato", "given_name": "Yuki" }, { "family_name": "Yamaguchi", "given_name": "Katsushi" }, { "family_name": "Zhang", "given_name": "Zicong" }, { "family_name": "Chihara", "given_name": "Akane", "orcid": "0000-0001-6984-615X" }, { "family_name": "Takehara", "given_name": "Mai" }, { "family_name": "Shibata", "given_name": "Yuki" }, { "family_name": "Kitada", "given_name": "Masaaki" }, { "family_name": "Moreno", "given_name": "Nerea" }, { "family_name": "Uchiyama", "given_name": "Ikuo", "orcid": "0000-0002-9197-5000" }, { "family_name": "Suzuki", "given_name": "Yutaka" }, { "family_name": "Takeuchi", "given_name": "Takashi" }, { "family_name": "Nikaido", "given_name": "Masato" }, { "family_name": "Agata", "given_name": "Kiyokazu", "orcid": "0000-0002-5195-2576" }, { "family_name": "Toyoda", "given_name": "Atsushi" }, { "family_name": "Shigenobu", "given_name": "Shuji", "orcid": "0000-0003-4640-2323" }, { "family_name": "Hayashi", "given_name": "Toshinori" }, { "family_name": "Suzuki", "given_name": "Ken-ichi T.", "orcid": "0000-0002-4502-831X" } ], "abstract": "Salamanders provide excellent models for studying vertebrate evolution, development, and regeneration. To further advance the newt as a model organism in biology, we conducted draft genome sequencing of 20 Gb of an inbred newt (Pleurodeles waltl). As part of this study, the Hoxd11\u2013d13 intergenic region is expanded by over 1 Mb owing to the massive insertion of repetitive sequences including newt-specific satellite DNA. Interestingly, Myod and Bmp4, genes that are typically involved in vertebrate development, are absent in salamanders. Co-option of Sodefrin Precursor-like Factor genes, which encode sex pheromone ligands, suggests a diversification of reproductive behavior among salamanders. Moreover, a limb enhancer of Shh, MFCS1/ZRS, retains its function even though it is positioned approximately 5 Mb away from the promoter. Furthermore, we have identified a functional cis-element potentially associated with limb regeneration in this enhancer. The newt genome yields crucial insights into amphibian evolution, behavior, development, and regeneration.", "doi": "10.1016/j.isci.2025.113535", "issn": "2589-0042", "publisher": "Cell Press", "publication": "iScience", "publication_date": "2025-10-17", "series_number": "10", "volume": "28", "issue": "10", "pages": "113535" }, { "id": "authors:5wvpp-hqf34", "collection": "authors", "collection_id": "5wvpp-hqf34", "cite_using_url": "https://authors.library.caltech.edu/records/5wvpp-hqf34", "type": "article", "title": "Fgf10 mutant newts regenerate normal hindlimbs despite severe developmental defects", "author": [ { "family_name": "Suzuki", "given_name": "Miyuki", "orcid": "0000-0002-7284-7505", "clpid": "Suzuki-Miyuki" }, { "family_name": "Okumura", "given_name": "Akinori", "orcid": "0000-0003-1080-6107", "clpid": "Okumura-Akinori" }, { "family_name": "Chihara", "given_name": "Akane", "orcid": "0000-0001-6984-615X", "clpid": "Chihara-Akane" }, { "family_name": "Shibata", "given_name": "Yuki", "orcid": "0000-0002-1437-6936", "clpid": "Shibata-Yuki" }, { "family_name": "Endo", "given_name": "Tetsuya", "orcid": "0000-0002-0056-9622", "clpid": "Endo-Tetsuya" }, { "family_name": "Teramoto", "given_name": "Machiko", "orcid": "0000-0002-5999-5810", "clpid": "Teramoto-Machiko" }, { "family_name": "Agata", "given_name": "Kiyokazu", "orcid": "0000-0002-5195-2576", "clpid": "Agata-Kiyokazu" }, { "family_name": "Bronner", "given_name": "Marianne E.", "orcid": "0000-0003-4274-1862", "clpid": "Bronner-M-E" }, { "family_name": "Suzuki", "given_name": "Ken-ichi T.", "orcid": "0000-0002-4502-831X", "clpid": "Suzuki-Ken-ichi-T" } ], "abstract": "In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function is broadly conserved in tetrapods and/or involved in adult limb regeneration remains unknown. To tackle this question, we established\n Fgf10\n mutant lines in the newt\n Pleurodeles waltl\n which has amazing regenerative ability. While\n Fgf10\n mutant forelimbs develop normally, the hindlimbs fail to develop and downregulate FGF target genes. Despite these developmental defects,\n Fgf10\n mutants were able to regenerate normal hindlimbs rather than recapitulating the embryonic phenotype. Together, our results demonstrate an important role for FGF10 in hindlimb formation, but little or no function in regeneration, suggesting that different mechanisms operate during limb regeneration versus development.", "doi": "10.1073/pnas.2314911121", "pmcid": "PMC10945807", "issn": "0027-8424", "publisher": "National Academy of Sciences", "publication": "Proceedings of the National Academy of Sciences", "publication_date": "2024-03-12", "series_number": "11", "volume": "121", "issue": "11", "pages": "e2314911121" } ]