[
    {
        "id": "authors:tbgg4-ac223",
        "collection": "authors",
        "collection_id": "tbgg4-ac223",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160510-141758758",
        "type": "article",
        "title": "Sequence-specific double-strand alkylation and cleavage of DNA mediated by triple-helix formation",
        "author": [
            {
                "family_name": "Povsic",
                "given_name": "Thomas J.",
                "clpid": "Povsic-T-J"
            },
            {
                "family_name": "Strobel",
                "given_name": "Scott A.",
                "clpid": "Strobel-S-A"
            },
            {
                "family_name": "Dervan",
                "given_name": "Peter B.",
                "orcid": "0000-0001-8852-7306",
                "clpid": "Dervan-P-B"
            }
        ],
        "abstract": "Attachment of the nondiffusible electrophile N-bromoacetyl to the 5-position of a thymine at the 5'-end of a pyrimidine oligodeoxyribonucleotide affords sequence specific alkylation of a guanine two base pairs to the 5'-side of a local triple-helix complex in >96% yield. N-Bromoacetyloligodeoxyribonucleotides bind adjacent inverted purine tracts on double-helical DNA by triple-helix formation and alkylate single guanine positions on opposite strands at 37-degrees-C (pH 7.4). After depurination, double-strand cleavage at a single site within plasmid DNA (4 kp in size) occurs in greater than 85% yield. The resulting DNA fragments from site-specific alkylation and cleavage can be ligated with DNA fragments generated by restriction endonuclease digestion. This nonenzymatic approach which couples sequence-specific recognition with sequence-dependent cleavage affords double-strand site-specific cleavage in megabase size DNA. A yeast chromosome, 340 000 base pairs in size, was cleaved at a single site in 85-90% yield.",
        "doi": "10.1021/ja00041a005",
        "issn": "0002-7863",
        "publisher": "American Chemical Society",
        "publication": "Journal of the American Chemical Society",
        "publication_date": "1992-07-15",
        "series_number": "15",
        "volume": "114",
        "issue": "15",
        "pages": "5934-5941"
    },
    {
        "id": "authors:3eckg-c1a51",
        "collection": "authors",
        "collection_id": "3eckg-c1a51",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160510-145543263",
        "type": "article",
        "title": "Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation",
        "author": [
            {
                "family_name": "Povsic",
                "given_name": "Thomas J.",
                "clpid": "Povsic-T-J"
            },
            {
                "family_name": "Dervan",
                "given_name": "Peter B.",
                "orcid": "0000-0001-8852-7306",
                "clpid": "Dervan-P-B"
            }
        ],
        "abstract": "Affinity cleaving, a method that relies on the attachment of a nonspecific cleaving moiety, such as EDTA\u2022Fe(ll), to a DNA binding molecule, facilitates the elucidation of the structural principles for DNA recognition. The determination of the  sequence specificities, groove locations, and binding orientations of peptide analogues, protein-DNA binding motifs, and oligonucleotide-triple-helix motifs has provided reliable models for the sequence-specific recognition of double-helical DNA. It now becomes possible to combine these binding molecules with domains capable of base-specific and quantitative modification of DNA (Figure 1). We report the design and synthesis of an  ligodeoxyribonucleotide equipped with an electrophile at the 5'-end that binds to double-helical DNA by triple-helix formation and alkylates predominantly at a single guanine base adjacent to the target DNA sequence in high yield.",
        "doi": "10.1021/ja00181a075",
        "issn": "0002-7863",
        "publisher": "American Chemical Society",
        "publication": "Journal of the American Chemical Society",
        "publication_date": "1990-12-05",
        "series_number": "25",
        "volume": "112",
        "issue": "25",
        "pages": "9428-9430"
    },
    {
        "id": "authors:4ry8w-rn191",
        "collection": "authors",
        "collection_id": "4ry8w-rn191",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160510-160429152",
        "type": "article",
        "title": "Triple helix formation by oligonucleotides on DNA extended to the physiological pH range",
        "author": [
            {
                "family_name": "Povsic",
                "given_name": "Thomas J.",
                "clpid": "Povsic-T-J"
            },
            {
                "family_name": "Dervan",
                "given_name": "Peter B.",
                "orcid": "0000-0001-8852-7306",
                "clpid": "Dervan-P-B"
            }
        ],
        "abstract": "We report here that oligodeoxyribonucleotides which contain 5-bromouracil (Br^5U) and 5-methylcytosine (m^5C) bind duplex DNA at the same homopurine target sequence as their T/C analogues but with greater affinities and over an extended pH range. Oligonucleotides containing uracil (U) bind with lower affinity (Figure 1).",
        "doi": "10.1021/ja00190a047",
        "issn": "0002-7863",
        "publisher": "American Chemical Society",
        "publication": "Journal of the American Chemical Society",
        "publication_date": "1989-04-12",
        "series_number": "8",
        "volume": "111",
        "issue": "8",
        "pages": "3059-3061"
    }
]