[ { "id": "authors:vcpvz-cyc44", "collection": "authors", "collection_id": "vcpvz-cyc44", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220916-663648000", "type": "article", "title": "Structure and Function of the Nuclear Pore Complex", "author": [ { "family_name": "Petrovic", "given_name": "Stefan", "orcid": "0000-0002-4979-8696", "clpid": "Petrovic-Stefan" }, { "family_name": "Mobbs", "given_name": "George W.", "orcid": "0000-0003-2405-3345", "clpid": "Mobbs-George-W" }, { "family_name": "Bley", "given_name": "Christopher J.", "orcid": "0000-0003-2301-1290", "clpid": "Bley-Christopher-J" }, { "family_name": "Nie", "given_name": "Si", "orcid": "0000-0002-1347-0656", "clpid": "Nie-Si" }, { "family_name": "Patke", "given_name": "Alina", "orcid": "0000-0003-1628-4282", "clpid": "Patke-Alina" }, { "family_name": "Hoelz", "given_name": "Andr\u00e9", "orcid": "0000-0003-1726-0127", "clpid": "Hoelz-A" } ], "abstract": "The nucleus, a genome-containing organelle eponymous of eukaryotes, is enclosed by a double membrane continuous with the endoplasmic reticulum. The nuclear pore complex (NPC) is an \u223c110-MDa, \u223c1000-protein channel that selectively transports macromolecules across the nuclear envelope and thus plays a central role in the regulated flow of genetic information from transcription to translation. Its size, complexity, and flexibility have hindered determination of atomistic structures of intact NPCs. Recent studies have overcome these hurdles by combining biochemical reconstitution and docking of high-resolution structures of NPC subcomplexes into cryo-electron tomographic reconstructions with biochemical and physiological validation. Here, we provide an overview of the near-atomic composite structure of the human NPC, a milestone toward unlocking a molecular understanding of mRNA export, NPC-associated diseases, and viral host\u2013pathogen interactions, serving as a paradigm for studying similarly large complexes.", "doi": "10.1101/cshperspect.a041264", "issn": "1943-0264", "publisher": "Cold Spring Harbor Laboratory Press", "publication": "Cold Spring Harbor Perspectives in Biology", "publication_date": "2022-12", "series_number": "12", "volume": "14", "issue": "12", "pages": "Art. No. a041264" }, { "id": "authors:36v8f-0a053", "collection": "authors", "collection_id": "36v8f-0a053", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20211102-155046319", "type": "article", "title": "Architecture of the cytoplasmic face of the nuclear pore", "author": [ { "family_name": "Bley", "given_name": "Christopher J.", "orcid": "0000-0003-2301-1290", "clpid": "Bley-Christopher-J" }, { "family_name": "Nie", "given_name": "Si", "orcid": "0000-0002-1347-0656", "clpid": "Nie-Si" }, { "family_name": "Mobbs", "given_name": "George W.", "orcid": "0000-0003-2405-3345", "clpid": "Mobbs-George-W" }, { "family_name": "Petrovic", "given_name": "Stefan", "orcid": "0000-0002-4979-8696", "clpid": "Petrovic-Stefan" }, { "family_name": "Gres", "given_name": "Anna T.", "orcid": "0000-0002-6915-5903", "clpid": "Gres-Anna-T" }, { "family_name": "Liu", "given_name": "Xiaoyu", "orcid": "0000-0002-6594-9677", "clpid": "Liu-Xiaoyu" }, { "family_name": "Mukherjee", "given_name": "Somnath", "orcid": "0000-0001-5447-4496", "clpid": "Mukherjee-Somnath" }, { "family_name": "Harvey", "given_name": "Sho", "orcid": "0000-0002-1742-4550", "clpid": "Harvey-Sho" }, { "family_name": "Huber", "given_name": "Ferdinand M.", "orcid": "0000-0001-8647-6971", "clpid": "Huber-Ferdinand-M" }, { "family_name": "Lin", "given_name": "Daniel H.", "orcid": "0000-0002-1393-3374", "clpid": "Lin-Daniel-H" }, { "family_name": "Brown", "given_name": "Bonnie", "clpid": "Brown-Bonnie" }, { "family_name": "Tang", "given_name": "Aaron W.", "orcid": "0000-0001-8891-6205", "clpid": "Tang-Aaron-W" }, { "family_name": "Rundlet", "given_name": "Emily J.", "orcid": "0000-0003-4041-6206", "clpid": "Rundlet-Emily-J" }, { "family_name": "Correia", "given_name": "Ana R.", "clpid": "Correia-Ana-R" }, { "family_name": "Chen", "given_name": "Shane", "clpid": "Che-Shane" }, { "family_name": "Regmi", "given_name": "Saroj G.", "orcid": "0000-0002-6705-3997", "clpid": "Regmi-Saroj-G" }, { "family_name": "Stevens", "given_name": "Taylor A.", "orcid": "0000-0002-6232-5316", "clpid": "Stevens-Taylor-A" }, { "family_name": "Jette", "given_name": "Claudia A.", "orcid": "0000-0002-5085-8027", "clpid": "Jette-Claudia-A" }, { "family_name": "Dasso", "given_name": "Mary", "orcid": "0000-0002-5410-1371", "clpid": "Dasso-Mary" }, { "family_name": "Patke", "given_name": "Alina", "orcid": "0000-0003-1628-4282", "clpid": "Patke-Alina" }, { "family_name": "Palazzo", "given_name": "Alexander F.", "orcid": "0000-0002-9700-1995", "clpid": "Palazzo-Alexander-F" }, { "family_name": "Kossiakoff", "given_name": "Anthony A.", "orcid": "0000-0003-3174-9359", "clpid": "Kossiakoff-Anthony-A" }, { "family_name": "Hoelz", "given_name": "Andr\u00e9", "orcid": "0000-0003-1726-0127", "clpid": "Hoelz-A" } ], "abstract": "The nuclear pore complex (NPC) is the sole bidirectional gateway for nucleocytoplasmic transport. Despite recent progress in elucidating the NPC symmetric core architecture, the asymmetrically decorated cytoplasmic face, essential for messenger RNA (mRNA) export and a hotspot for nucleoporin-associated diseases, has remained elusive. Here we report a composite structure of the human cytoplasmic face obtained by combining biochemical reconstitution, crystal structure determination, docking into cryo\u2013electron tomographic reconstructions, and physiological validation. Whereas species-specific motifs anchor an evolutionarily conserved ~540-kilodalton heterohexameric cytoplasmic filament nucleoporin complex above the central transport channel, attachment of the NUP358 pentameric bundles depends on the double-ring arrangement of the coat nucleoporin complex. Our composite structure and its predictive power provide a rich foundation for elucidating the molecular basis of mRNA export and nucleoporin diseases.", "doi": "10.1126/science.abm9129", "pmcid": "PMC9348906", "issn": "0036-8075", "publisher": "American Association for the Advancement of Science", "publication": "Science", "publication_date": "2022-06-10", "series_number": "6598", "volume": "376", "issue": "6598", "pages": "Art. No. abm9129" } ]