[
    {
        "id": "authors:mdtpv-vrs47",
        "collection": "authors",
        "collection_id": "mdtpv-vrs47",
        "cite_using_url": "https://authors.library.caltech.edu/records/mdtpv-vrs47",
        "type": "article",
        "title": "Recruiting ESCRT to single-chain heterotrimer peptide MHCI releases antigen-presenting vesicles that stimulate T cells selectively",
        "author": [
            {
                "family_name": "Olson",
                "given_name": "Blade A.",
                "orcid": "0000-0002-1526-1399",
                "clpid": "Olson-Blade-A"
            },
            {
                "family_name": "Huey-Tubman",
                "given_name": "Kathryn E.",
                "orcid": "0000-0002-4683-8138",
                "clpid": "Huey-Tubman-Kathryn-E"
            },
            {
                "family_name": "Mao",
                "given_name": "Zhiyuan"
            },
            {
                "family_name": "Hoffmann",
                "given_name": "Magnus A. G.",
                "orcid": "0000-0003-4923-9568",
                "clpid": "Hoffmann-Magnus-Adrian-Gero"
            },
            {
                "family_name": "Bjorkman",
                "given_name": "Pamela J.",
                "orcid": "0000-0002-2277-3990",
                "clpid": "Bjorkman-P-J"
            },
            {
                "family_name": "Murray",
                "given_name": "Richard M.",
                "orcid": "0000-0002-5785-7481",
                "clpid": "Murray-R-M"
            },
            {
                "family_name": "Mayo",
                "given_name": "Stephen L.",
                "orcid": "0000-0002-9785-5018",
                "clpid": "Mayo-S-L"
            }
        ],
        "abstract": "Immune cells naturally secrete extracellular antigen-presenting vesicles (APVs) displaying peptide:MHC complexes to facilitate the initiation, expansion, maintenance, or silencing of immune responses. Previous work has sought to manufacture and purify these vesicles for cell-free immunotherapies. In this study, APV assembly and release is achieved in nonimmune cells by transfecting a single-chain heterotrimer (SCT) peptide major histocompatibility complex I (pMHCI) construct containing an ESCRT- and ALIX-binding region (EABR) sequence appended to the cytoplasmic tail; this EABR sequence recruits ESCRT proteins to induce the budding of APVs displaying SCT pMHCI. A comparison of multiple pMHCI constructs shows that inducing the release of APVs by the addition of an EABR sequence generalizes across SCT pMHCI constructs. Purified pMHCI/EABR APVs selectively stimulate IFN-\u03b3 release from T cells presenting their cognate T cell receptor, demonstrating the potential use of these vesicles as a form of cell-free immunotherapy.",
        "doi": "10.1073/pnas.2501046122",
        "pmcid": "PMC12745732",
        "issn": "0027-8424",
        "publisher": "National Academy of Sciences",
        "publication": "Proceedings of the National Academy of Sciences",
        "publication_date": "2025-12-23",
        "series_number": "51",
        "volume": "122",
        "issue": "51",
        "pages": "e2501046122"
    }
]