[
    {
        "id": "thesis:3072",
        "collection": "thesis",
        "collection_id": "3072",
        "cite_using_url": "https://resolver.caltech.edu/CaltechETD:etd-08092006-124948",
        "primary_object_url": {
            "basename": "Roller_a_1961.pdf",
            "content": "final",
            "filesize": 4083686,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/3072/1/Roller_a_1961.pdf",
            "version": "v2.0.0"
        },
        "type": "thesis",
        "title": "Studies on the Replication and Transfer to Progeny of the DNA of the Bacteriophage T4",
        "author": [
            {
                "family_name": "Roller",
                "given_name": "Ann",
                "clpid": "Roller-Ann"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Meselson",
                "given_name": "Matthew S.",
                "clpid": "Meselson-M-S"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Unknown",
                "given_name": "Unknown"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "The purpose of the present research has been to learn more about the mechanism of replication and ultimate fate of the DNA of the phage T4. The distribution of parental material in DNA molecules in the replicating pool and in the DNA obtained from progeny phage has been examined. The association of parental atoms with the r genotype has also been examined.\r\n\r\nPhage in which the nitrogen and carbon we re replaced by the stable heavy isotopes N15 and C13 were used to infect unlabeled bacteria. At various times after infection, aliquots of the culture were removed and lysed; finally, progeny phage were harvested and lysed. DNA molecules containing heavy atoms show a corresponding increase in their density. Separation is achieved by density-gradient centrifugation.\r\n\r\nMost parental atoms transferred to progeny are found in DNA of light density. Following fragmentation, DNA containing parental atoms is found to have shifted to densities between those of light and half-heavy DNA. In the replicating pool, half-heavy DNA is also found. It is concluded that transferred DNA is incorporated into hybrid units which are attached to larger segments of completely new DNA. The parental atoms may comprise a part of one strand of a duplex DNA molecule.\r\n\r\nAn analysis was made of the density distribution of the viable progeny of a multiple infection with T4r and r+, one of which was labeled with N15 and C13. At densities greater than that of the average progeny, relatively more progeny of the genotype of the labeled parent are found. Thus, parental atoms tend to remain associated with parental genotype.",
        "doi": "10.7907/DN8N-DF94",
        "publication_date": "1961",
        "thesis_type": "phd",
        "thesis_year": "1961"
    },
    {
        "id": "thesis:6315",
        "collection": "thesis",
        "collection_id": "6315",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:04132011-072837251",
        "primary_object_url": {
            "basename": "Rolfe_re_1961.pdf",
            "content": "final",
            "filesize": 23380868,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/6315/1/Rolfe_re_1961.pdf",
            "version": "v4.0.0"
        },
        "type": "thesis",
        "title": "I. The Relative Homogeneity of Microbial Desoxyribonucleic Acid. II. The Molecular Arrangement of the Conserved Subunits of the Desoxyribonucleic Acid of Escherichia coli",
        "author": [
            {
                "family_name": "Rolfe",
                "given_name": "Ronald Efrom",
                "clpid": "Rolfe-Ronald-Efrom"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Meselson",
                "given_name": "Matthew S.",
                "clpid": "Meselson-M-S"
            },
            {
                "family_name": "Pauling",
                "given_name": "Linus",
                "clpid": "Pauling-L"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Unknown",
                "given_name": "Unknown"
            }
        ],
        "local_group": [
            {
                "literal": "div_chem"
            }
        ],
        "abstract": "I. The buoyant density of DNA in a CsCl-density gradient has been shown to depend on its nucleotide composition. The linear relationship has been used to study the distribution of nucleotide compositions among the DNA molecules isolated from a single microbial species. Each microbial DNA has been shown to be unusually homogeneous relative to the range of compositions found for DNA isolated from different species. The relevance of these findings to current views of the function of DNA in biological systems is discussed.\r\n\r\nII. The molecular arrangement of the conserved subunits of E. coli DNA has been investigated by examining molecular fragments of hybrid ^(13)C, ^(15)N E. coli DNA. Two extreme models for the arrangement of subunits, the side-to \u2013side model, and the end-to-end model, were considered. Predictions regarding CsCl-density-gradient distribution for fragments of hybrid DNA were developed for each model. These predictions were based on a theoretical analysis, but utilized pertinent experimental data obtained from a study of unlabelled E. coli DNA. Comparison of theory with experiment indicated that the end-to-end model is incorrect, and set an upper limit to the amount of fully labeled fragments released when hybrid DNA is sonicated.\r\n",
        "doi": "10.7907/CBDY-KV20",
        "publication_date": "1961",
        "thesis_type": "phd",
        "thesis_year": "1961"
    }
]