[ { "id": "https://authors.library.caltech.edu/records/edrvt-vx355", "eprint_id": 57209, "eprint_status": "archive", "datestamp": "2023-08-22 14:23:30", "lastmod": "2023-10-23 17:09:43", "type": "conference_item", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mariita-R-M", "name": { "family": "Mariita", "given": "R. M." } }, { "id": "Bhatnagar-S", "name": { "family": "Bhatnagar", "given": "S." } }, { "id": "Hanselmann-K", "name": { "family": "Hanselmann", "given": "K." } }, { "id": "Hossain-Mohammad-J", "name": { "family": "Hossain", "given": "M." }, "orcid": "0000-0003-3848-9035" }, { "id": "Liles-M", "name": { "family": "Liles", "given": "M." } }, { "id": "Moss-A-G", "name": { "family": "Moss", "given": "A. G." } }, { "id": "Leadbetter-J-R", "name": { "family": "Leadbetter", "given": "J. R." }, "orcid": "0000-0002-7033-0844" }, { "id": "Newman-D-K", "name": { "family": "Newman", "given": "D. K." }, "orcid": "0000-0003-1647-1918" } ] }, "title": "Characterization, Comparative Genomics and Genome Mining for Antibiotics and Secondary Metabolite of two Actinomycetales isolates", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2014 American Society for Cell Biology. TUESDAY-POSTER PRESENTATION.\n\n2014 Microbial Diversity, Marine Biological Laboratory. Pacific Biotechnology. RMM support:\nAU-CMB Peaks of Excellence summer graduate research award, Selman A. Waksman Endowed Scholarship in Microbial Diversity, Bernard Davis Endowed Scholarship (47802012050), Auburn Graduate School, and the AU-DBS Farrington Fund. We are grateful to Scott Dawson, George OToole, Alison\nBulter, Emil Ruff, Arpita Bose and Suzanne Kern for their assistance.\n\n
Published - Mariita_2014pP2378.pdf
", "abstract": "Actinomycetes are ubiquitous Gram (+) bacteria commonly found to have high G+C content and best\nknown for their metabolic by-products and novel enzymes [1]. Isolates CCMMD2014 & MRMD2014\nwere co-cultured from soil impacted by a rusty fire hydrant in Woods Hole, MA. The Streptomyces sp.\nand Curtobacterium sp. isolates were identified by marker genes for 16S rRNA, rpoB, xylose isomerase,\ntryptophan synthase beta chain and Cytochrome P450 monooxygenase. Both isolates showed lactic acid\nfermentation and urease activity. The co-isolates were separated by selective culturing with antibiotics.\nIn addition, whole genome sequencing revealed distinct inherent metabolic pathways in each culture\nthat allowed for mutually exclusive selective culture conditions. Assembly was done using HGAP3 with\nCelera8 assembler using SMRT portal [2,3]. Annotation was done using the RAST server [4], with 7540\nand 3969 CDS for Streptomyces sp. and Curtobacterium sp. respectively being revealed by AMIGene and\nBASys [5,6]. Subsequently, antiSMASH [7], was used to predict 52 and 26 secondary metabolite\nbiosynthetic clusters that included genes for lantipeptides, terpenes, siderophores, polyketide synthases\ntype I and II, bacteriocin and nonribosomal peptide synthase genes for Streptomyces sp. and\nCurtobacterium sp. respectively. The isolates have genes of potentially beneficial traits that could help\nstudy, among others, the role of fimbrial adhesins and iron in biofilm formation and investigation on\nnatural products.", "date": "2014-12", "date_type": "published", "publisher": "Caltech Library", "id_number": "CaltechAUTHORS:20150504-143203439", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150504-143203439", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "AU-CMB Peaks of Excellence summer graduate research award" }, { "agency": "Selman A. Waksman Endowed Scholarship in Microbial Diversity" }, { "agency": "Auburn Graduate School, Bernard Davis Endowed Scholarship", "grant_number": "47802012050" }, { "agency": "AU-DBS Farrington Fund" } ] }, "local_group": { "items": [ { "id": "Division-of-Geological-and-Planetary-Sciences" } ] }, "contributors": { "items": [ { "id": "Lippincott-Schwartz-J", "name": { "family": "Lippincott-Schwartz", "given": "Jennifer" } }, { "id": "Marshall-W", "name": { "family": "Marshall", "given": "Wallace" } }, { "id": "Marks-M", "name": { "family": "Marks", "given": "Michael" } } ] }, "primary_object": { "basename": "Mariita_2014pP2378.pdf", "url": "https://authors.library.caltech.edu/records/edrvt-vx355/files/Mariita_2014pP2378.pdf" }, "resource_type": "conference_item", "pub_year": "2014", "author_list": "Mariita, R. M.; Bhatnagar, S.; et el." } ]