[
    {
        "id": "thesis:7334",
        "collection": "thesis",
        "collection_id": "7334",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:12132012-153519784",
        "primary_object_url": {
            "basename": "Jongeward_gd_1993.pdf",
            "content": "final",
            "filesize": 42474088,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/7334/1/Jongeward_gd_1993.pdf",
            "version": "v5.0.0"
        },
        "type": "thesis",
        "title": "Negative regulators of the let-23 EGF receptor in Caenorhabditis elegans vulval differentiation",
        "author": [
            {
                "family_name": "Jongeward",
                "given_name": "Gregg D.",
                "clpid": "Jongeward-G-D"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Sternberg",
                "given_name": "Paul W.",
                "orcid": "0000-0002-7699-0173",
                "clpid": "Sternberg-P-W"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Sternberg",
                "given_name": "Paul W.",
                "orcid": "0000-0002-7699-0173",
                "clpid": "Sternberg-P-W"
            },
            {
                "family_name": "Lipshitz",
                "given_name": "Howard D.",
                "orcid": "0000-0002-7372-4419",
                "clpid": "Lipshitz-H-D"
            },
            {
                "family_name": "Lewis",
                "given_name": "Edward B.",
                "clpid": "Lewis-E-B"
            },
            {
                "family_name": "Anderson",
                "given_name": "David J.",
                "orcid": "0000-0001-6175-3872",
                "clpid": "Anderson-D-J"
            },
            {
                "family_name": "Emr",
                "given_name": "Scott D.",
                "orcid": "0000-0002-5408-6781",
                "clpid": "Emr-S-D"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "<p>Vulval induction in C. elegans is an example of the use of an EGF\r\n(Epidermal Growth Factor) mediated signal transduction system. At least\r\nfive genes are involved in the negative regulation of vulval induction.</p>\r\n\r\n<p>Mutations at the silent locus sli-1 (suppressor of lineage defect) are\r\nsufficient to suppress all of the phenotypes associated with hypomorphic\r\nalleles of let-23. sli-1 functions to modify the activity of let-23 but muations\r\nat sli-1 do not bypass the requirement for let-23. Based on the phenotypes of\r\nanimals bearing mutations at sli-1 and other genes, sli-1 may function at or\r\nnear the let-23 or sem-5 step of vulval differentiation.</p>\r\n\r\n<p>Null alleles of the pleiotropic locus unc-101 cause a number of mutant\r\nphenotypes including neural defects and suppression of the vulval defects\r\nassociated with some weak let-23 mutations. These unc-101 mutations\r\ninteract with mutations in other genes required for proper vulval\r\ndifferentiation but do not act as generalized suppressors. This locus has been\r\ncloned and encodes the C. elegans homolog of the Golgi-associated clathrin\r\nadaptor protein AP47.</p>\r\n\r\n<p>Animals mutant for both unc-101 and sli-1 display excessive vulval\r\ndifferentiation. Animals mutant at only one of these loci display no vulval\r\nabnormalities. This excessive vulval differentiation requires the inductive\r\nsignal and functionallet-23, suggesting that sli-1 and unc-101 function to\r\nnegatively regulate the response to the inductive signal, rather than the\r\nbasal activity of let-23.</p>\r\n\r\n<p>Rare mutant alleles at lin-2, lin-7, and let-23 result in excessive vulval\r\ndifferentiation. These alleles are genetically similar to more common alleles\r\nof these genes which result in the failure to differentiate vulval tissue. These\r\nthree genes apparently are required for the activation of both positive and negative regulators of vulval differentiation.</p>\r\n\r\n<p>A number of negative regulators function to control the activity of let-23.\r\nAt least three pathaways of negative regulation have been genetically\r\nidentified. These negative regulators act to limit the response to a growth or\r\ndifferentiation factor.</p>\r\n",
        "doi": "10.7907/87ea-tr80",
        "publication_date": "1993",
        "thesis_type": "phd",
        "thesis_year": "1993"
    }
]