[ { "id": "authors:wq9k4-t5965", "collection": "authors", "collection_id": "wq9k4-t5965", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180711-141559479", "type": "book_section", "title": "Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays", "book_title": "Smart Biomedical and Physiological Sensor Technology XI", "author": [ { "family_name": "Coppock", "given_name": "Matthew B.", "clpid": "Coppock-M-B" }, { "family_name": "Farrow", "given_name": "Blake", "clpid": "Farrow-B" }, { "family_name": "Warner", "given_name": "Candice", "clpid": "Warner-C-R" }, { "family_name": "Finch", "given_name": "Amethist S.", "clpid": "Finch-A-S" }, { "family_name": "Lai", "given_name": "Bert", "clpid": "Lai-Bert-T" }, { "family_name": "Sarkes", "given_name": "Deborah A.", "clpid": "Sarkes-D-A" }, { "family_name": "Heath", "given_name": "James R.", "orcid": "0000-0001-5356-4385", "clpid": "Heath-J-R" }, { "family_name": "Stratis-Cullum", "given_name": "Dimitra", "clpid": "Stratis-Cullum-D-N" } ], "contributor": [ { "family_name": "Cullum", "given_name": "Brian M.", "clpid": "Cullun-B-M" }, { "family_name": "McLamore", "given_name": "Eric S.", "clpid": "McLamore-E-S" } ], "abstract": "Current biodetection assays that employ monoclonal antibodies as primary capture agents exhibit limited fieldability, shelf life, and performance due to batch-to-batch production variability and restricted thermal stability. In order to improve upon the detection of biological threats in fieldable assays and systems for the Army, we are investigating protein catalyzed capture (PCC) agents as drop-in replacements for the existing antibody technology through iterative in situ click chemistry. The PCC agent oligopeptides are developed against known protein epitopes and can be mass produced using robotic methods. In this work, a PCC agent under development will be discussed. The performance, including affinity, selectivity, and stability of the capture agent technology, is analyzed by immunoprecipitation, western blotting, and ELISA experiments. The oligopeptide demonstrates superb selectivity coupled with high affinity through multi-ligand design, and improved thermal, chemical, and biochemical stability due to non-natural amino acid PCC agent design", "doi": "10.1117/12.2052542", "isbn": "9781628410440", "publisher": "Society of Photo-optical Instrumentation Engineers (SPIE)", "place_of_publication": "Bellingham, WA", "publication_date": "2014-05-22", "pages": "Art. no. 910711" }, { "id": "authors:8w6kc-06a42", "collection": "authors", "collection_id": "8w6kc-06a42", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180402-144708576", "type": "book_section", "title": "Label-Free Nanowire and Nanotube Biomolecular Sensors for In-Vitro Diagnosis of Cancer and other Diseases", "book_title": "Nanobiotechnology II", "author": [ { "family_name": "Heath", "given_name": "James R.", "orcid": "0000-0001-5356-4385", "clpid": "Heath-J-R" } ], "contributor": [ { "family_name": "Mirkin", "given_name": "Chad A.", "clpid": "Mirkin-C-A" }, { "family_name": "Niemeyer", "given_name": "Christof M.", "clpid": "Niemeyer-C-M" } ], "abstract": "[no abstract]", "doi": "10.1002/9783527610389.ch12", "isbn": "9783527316731", "publisher": "Wiley", "place_of_publication": "Weinheim", "publication_date": "2007-01-26", "pages": "213-232" }, { "id": "authors:xqqr4-5nt64", "collection": "authors", "collection_id": "xqqr4-5nt64", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170516-174653680", "type": "book_section", "title": "Molecular mechanics and molecular electronics", "book_title": "5th IEEE Conference on Nanotechnology, 2005", "author": [ { "family_name": "Heath", "given_name": "James R.", "orcid": "0000-0001-5356-4385", "clpid": "Heath-J-R" } ], "abstract": "Electronic devices containing molecules as either passive or active (switching) components present the opportunity for scaling electronic circuitry down to near-molecular dimensions. In this paper the kinetic and thermodynamic properties of bistable molecular mechanical switches known as catenanes and rotaxanes are discussed. A defect-tolerant, binary tree demultiplexer architecture using Order log/sub 2/N submicron (lithographically patterned) wires to address TV nanowires are developed. Apart from traditional applications of memory, logic, and routing, new opportunities that include actuation, sensing, energy management, and possibly even peptide sequencing are enabled by these nanofabrication approaches.", "doi": "10.1109/NANO.2005.1500634", "isbn": "0-7803-9199-3", "publisher": "IEEE", "place_of_publication": "Piscataway, NJ", "publication_date": "2005-07" }, { "id": "authors:dgj0r-1fd64", "collection": "authors", "collection_id": "dgj0r-1fd64", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20161102-163632579", "type": "book_section", "title": "A Systems Approach to Molecular Electronics", "book_title": "ISLPED '03 Proceedings of the 2003 international symposium on Low power electronics and design", "author": [ { "family_name": "Heath", "given_name": "James R.", "orcid": "0000-0001-5356-4385", "clpid": "Heath-J-R" } ], "contributor": [ { "family_name": "Verbauwhede", "given_name": "Ingrid", "clpid": "Verbauwhede-I" }, { "family_name": "Roh", "given_name": "Hyung", "clpid": "Roh-Hyung" } ], "abstract": "Molecular electronics is an area of research in which molecules are employed to yield the active and passive device components (switches, diodes, resistors) of an electronic circuit or integrated circuit. Consider the crossbar circuits of nanowires in the electron micrograph at the left [1]. The smallest (100 element) crossbar in this image is patterned at a density approach 10^12/cm^2, and the wire diameter is approximately 8 nm. At a doping level (with species like Boron or Arsenic) of 10^18/cm^3, a similar 8 nm diameter, micrometer-long segment of silicon wires would have 20-30 dopant atoms, and a junction of two crossed wires would contain approximately 0.1-0.2 dopant atoms. Thus, field-effect transistors fabricated at these wiring densities might exhibit non-statistical, and perhaps non-predictable behavior. Related arguments, such as the gate oxide thickness, power consumption, (just from leakage currents through the gate oxide), fabrication costs, and others also highlight the difficulty of scaling standard electronics materials to molecular dimensions [2]. The point is that at device areas of a few tens of square nanometers, molecules have a certain fundamental attractiveness because of their size, because they represent the ultimate in terms of atomic control over physical properties, and because of the diversity of properties, such as switching, dynamic organization and recognition that can be achieved through such control.Molecular electronics circuits based on crossbar architectures can be utilized for both logic and memory applications [3], but in order to realize such applications, many things must be simultaneously considered. These include the design of the molecule, the molecule electrode interface, electronically configurable and defect tolerant circuit architectures, methods for bridging the nanometer-scale densities of these circuits to the sub-micrometer densities achievable with lithography, etc. [4] In this talk I will treat such circuits as a system, and discuss how all of these various properties are interrelated. I will also present experimental results of working random-access memory and configurable logic circuits, and FET-based multiplexers capable of bridging length scales.In these circuits the active device elements are molecular mechanical complexes organized at each of the junctions within the crossbar, as shown at left in the drawing. The molecules are switched via 1 or 2 electron process that results in a mechanical isomerization of the molecule, and thereby a change in the tunneling characteristics of the junction. Detailed electrical measurements on single molecule, three-terminal devices are revealing substantial information concerning how these types of devices can be better designed and optimized, and such measurements will also be presented and discussed.", "doi": "10.1145/871506.871596", "isbn": "1-58113-682-X", "publisher": "ACM", "place_of_publication": "New York, NY", "publication_date": "2003-08", "pages": "359" }, { "id": "authors:7xdxf-4hr31", "collection": "authors", "collection_id": "7xdxf-4hr31", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180302-094051482", "type": "book_section", "title": "Synthesis of C_(60) from Small Carbon Clusters", "book_title": "Fullerenes", "author": [ { "family_name": "Heath", "given_name": "James R.", "orcid": "0000-0001-5356-4385", "clpid": "Heath-J-R" } ], "contributor": [ { "family_name": "Hammond", "given_name": "George S.", "clpid": "Hammond-G-S" }, { "family_name": "Kuck", "given_name": "Valerie J.", "clpid": "Kuck-V-J" } ], "abstract": "A model based on experiment and ab initio theory for the high-yield carbon-arc synthesis of C_(60) and other fullerenes is presented. Evidence that is given indicates that the synthesis must start with the smallest units of carbon (atoms, dimers, etc.). The model is then broken into four steps: (1) the growth of carbon chains up to length C_(10) from initial reactants present in the carbon vapor, (2) growth from chains into monocyclic rings (C_(10)\u2014C_(20)), (3) production and growth of three-dimensional reactive carbon networks (C_(21)\u2014C_x, x = 30\u201440), and (4) growth of small fullerene cages via a closed-shell mechanism that exclusively produces C_(60), C_(70), and the higher fullerenes as the stable products.", "doi": "10.1021/bk-1992-0481.ch001", "isbn": "9780841221826", "publisher": "American Chemical Society", "place_of_publication": "Washington, DC", "publication_date": "1992-01-06", "pages": "1-23" } ]