[ { "id": "https://authors.library.caltech.edu/records/c5an0-87j84", "eprint_id": 98126, "eprint_status": "archive", "datestamp": "2023-08-19 14:47:47", "lastmod": "2024-01-14 21:53:23", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Arbabi-E", "name": { "family": "Arbabi", "given": "Ehsan" }, "orcid": "0000-0002-5328-3863" }, { "id": "Li-Jiaqi", "name": { "family": "Li", "given": "Jiaqi" }, "orcid": "0000-0003-2021-2310" }, { "id": "Hutchins-R-J", "name": { "family": "Hutchins", "given": "Romanus J." } }, { "id": "Kamali-S-M", "name": { "family": "Kamali", "given": "Seyedeh Mahsa" }, "orcid": "0000-0002-6968-811X" }, { "id": "Arbabi-A", "name": { "family": "Arbabi", "given": "Amir" }, "orcid": "0000-0001-8831-7552" }, { "id": "Horie-Yu", "name": { "family": "Horie", "given": "Yu" }, "orcid": "0000-0001-7083-1270" }, { "id": "Van-Dorpe-P", "name": { "family": "Van Dorpe", "given": "Pol" } }, { "id": "Gradinaru-V", "name": { "family": "Gradinaru", "given": "Viviana" }, "orcid": "0000-0001-5868-348X" }, { "id": "Wagenaar-D-A", "name": { "family": "Wagenaar", "given": "Daniel A." }, "orcid": "0000-0002-6222-761X" }, { "id": "Faraon-A", "name": { "family": "Faraon", "given": "Andrei" }, "orcid": "0000-0002-8141-391X" } ] }, "title": "Double-wavelength metasurface objective lens for two-photon microscopy (Conference Presentation)", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2019 Society of Photo-Optical Instrumentation Engineers (SPIE).", "abstract": "Two-photon microscopy is a key imaging technique in biological sciences because of its superior deep tissue imaging capabilities in addition to high transverse and axial resolution. In recent years, development of low-weight miniature two-photon microscopes has been of great interest for in vivo imaging of brain activity. Limited by these mechanical constraints, most of the developed miniature two-photon microscopes utilize graded index objective lenses that usually have inferior optical characteristics compared to conventional refractive objective lenses. Dielectric metasurfaces, a recent category of diffractive optical elements with enhanced capabilities, have proven versatile in various applications ranging from lensing to holography and polarization control. Their ultrathin form factor and potentially extremely low-weight make them very attractive for applications with stringent size and weight constraints. However, despite their success in various types of microscopy and imaging applications, they have not been previously utilized for multi-photon fluorescence microscopy. The main barrier for using metasurface lenses in multi-photon microscopy arises from their large chromatic dispersion that effectively makes them single-wavelength. Here we will present a double-wavelength metasurface lens especially designed to have the same focal length at 820 and 605 nm, corresponding to the excitation and emission wavelengths of a certain fluorophore. After characterizing the poly-silicon metasurface lens at both wavelengths, we used it in a two-photon microscopy setup and demonstrated its capability to capture two-photon images qualitatively similar to images taken with a conventional objective lens. We will also discuss the effects of chromatic dispersion of the metasurface lens on its two-photon imaging performance.", "date": "2019-03-08", "date_type": "published", "publisher": "Society of Photo-optical Instrumentation Engineers (SPIE)", "place_of_pub": "Bellingham, WA", "pagerange": "Art. No. 109280K", "id_number": "CaltechAUTHORS:20190822-134054530", "isbn": "9781510624986", "book_title": "High Contrast Metastructures VIII", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190822-134054530", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Chang-Hasnain-C-J", "name": { "family": "Chang-Hasnain", "given": "Connie J." } }, { "id": "Faraon-A", "name": { "family": "Faraon", "given": "Andrei" } }, { "id": "Zhou-Weimin", "name": { "family": "Zhou", "given": "Weimin" } } ] }, "doi": "10.1117/12.2510596", "resource_type": "book_section", "pub_year": "2019", "author_list": "Arbabi, Ehsan; Li, Jiaqi; et el." }, { "id": "https://authors.library.caltech.edu/records/fwk1q-xbm38", "eprint_id": 87751, "eprint_status": "archive", "datestamp": "2023-08-19 07:56:20", "lastmod": "2024-01-14 20:23:48", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Brake-J-H", "name": { "family": "Brake", "given": "Joshua" }, "orcid": "0000-0002-5113-6886" }, { "id": "Ruan-Haowen", "name": { "family": "Ruan", "given": "Haowen" }, "orcid": "0000-0002-4917-4509" }, { "id": "Robinson-J-E", "name": { "family": "Robinson", "given": "J. Elliott" }, "orcid": "0000-0001-9417-3938" }, { "id": "Liu-Yan", "name": { "family": "Liu", "given": "Yan" }, "orcid": "0000-0002-5837-4908" }, { "id": "Gradinaru-V", "name": { "family": "Gradinaru", "given": "Viviana" }, "orcid": "0000-0001-5868-348X" }, { "id": "Yang-Changhuei", "name": { "family": "Yang", "given": "Changhuei" }, "orcid": "0000-0001-8791-0354" } ] }, "title": "Time-reversed ultrasonically encoded (TRUE) focusing for deep-tissue optogenetic modulation", "ispublished": "unpub", "full_text_status": "public", "keywords": "wavefront shaping, optogenetics, brain, time-reversed ultrasonically encoded (TRUE) focusing, time-reversal, scattering, biophotonics, optical phase conjugation", "note": "\u00a9 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).\n\n
Published - 1050210.pdf
", "abstract": "The problem of optical scattering was long thought to fundamentally limit the depth at which light could be focused through turbid media such as fog or biological tissue. However, recent work in the field of wavefront shaping has demonstrated that by properly shaping the input light field, light can be noninvasively focused to desired locations deep inside scattering media. This has led to the development of several new techniques which have the potential to enhance the capabilities of existing optical tools in biomedicine. Unfortunately, extending these methods to living tissue has a number of challenges related to the requirements for noninvasive guidestar operation, speed, and focusing fidelity. Of existing wavefront shaping methods, time-reversed ultrasonically encoded (TRUE) focusing is well suited for applications in living tissue since it uses ultrasound as a guidestar which enables noninvasive operation and provides compatibility with optical phase conjugation for high-speed operation. In this paper, we will discuss the results of our recent work to apply TRUE focusing for optogenetic modulation, which enables enhanced optogenetic stimulation deep in tissue with a 4-fold spatial resolution improvement in 800-micron thick acute brain slices compared to conventional focusing, and summarize future directions to further extend the impact of wavefront shaping technologies in biomedicine.", "date": "2018-02-23", "date_type": "published", "publisher": "Society of Photo-optical Instrumentation Engineers (SPIE)", "place_of_pub": "Bellingham, WA", "pagerange": "Art. No. 1050210", "id_number": "CaltechAUTHORS:20180711-103004538", "isbn": "9781510614895", "book_title": "Adaptive Optics and Wavefront Control for Biological Systems IV", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180711-103004538", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Bifano-T-G", "name": { "family": "Bifano", "given": "Thomas G." } }, { "id": "Kubby-J", "name": { "family": "Kubby", "given": "Joel" } }, { "id": "Gigan-S", "name": { "family": "Gigan", "given": "Sylvain" } } ] }, "doi": "10.1117/12.2288331", "primary_object": { "basename": "1050210.pdf", "url": "https://authors.library.caltech.edu/records/fwk1q-xbm38/files/1050210.pdf" }, "resource_type": "book_section", "pub_year": "2018", "author_list": "Brake, Joshua; Ruan, Haowen; et el." } ]