[
    {
        "id": "authors:3w8s5-7es75",
        "collection": "authors",
        "collection_id": "3w8s5-7es75",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:EGBjvir69",
        "type": "article",
        "title": "Characteristics of the Deoxyribonucleic Acid of T\u03c63, a Bacteriophage for Bacillus stearothermophilus",
        "author": [
            {
                "family_name": "Egbert",
                "given_name": "Larre N.",
                "clpid": "Egbert-L-N"
            }
        ],
        "abstract": "It is shown that the individual strands of bacteriophage T\u03c63 DNA are intact and that heat-denatured T\u03c63 DNA forms a bimodal distribution in a neutral CsCl density gradient.",
        "pmcid": "PMC375805",
        "issn": "0022-538X",
        "publisher": "American Society for Microbiology",
        "publication": "Journal of Virology",
        "publication_date": "1969-05",
        "series_number": "5",
        "volume": "3",
        "issue": "5",
        "pages": "528-532"
    },
    {
        "id": "authors:8be5j-83692",
        "collection": "authors",
        "collection_id": "8be5j-83692",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:EGBjvir67a",
        "type": "article",
        "title": "Characteristics of T\u03c63, a Bacteriophage for Bacillus stearothermophilus",
        "author": [
            {
                "family_name": "Egbert",
                "given_name": "Larre N.",
                "clpid": "Egbert-L-N"
            },
            {
                "family_name": "Mitchell",
                "given_name": "Herschel K.",
                "clpid": "Mitchell-H-K"
            }
        ],
        "abstract": "A bacteriophage (T\u03c63) which infects the thermophilic bacterium Bacillus stearothermophilus ATCC 8005 was isolated and characterized. Infection of the bacterium by the bacteriophage was carried out at 60 C, the optimal growth temperature of the host. At 60 C, the phage had a latent period of 18 min and a burst size of about 200. The phage was comparatively thermostable in broth. The halflife of T\u03c63 was 400 min at 60 C, 120 min at 65 C, 40 min at 70 C, and 12 min at 75 C. The activation energy for the heat inactivation of T\u03c63 was 56,000 cal. The buoyant density of T\u03c63 in a cesium chloride density gradient was 1.526 g/ml. Electron micrographs of T\u03c63 indicate that the phage has a head that is 57 m\u00b5 long. The dimensions and shape of the head are compatible with those of a regular icosahedron. Each edge of the head is 29 m\u00b5 long. The tail of T\u03c63 is 125 m\u00b5 long and 10 m\u00b5 wide. There are about 30 cross-striations that are spaced at 3.9-m\u00b5 intervals along the tail. Under the conditions investigated, T\u03c63 adsorbed slowly to the host. Only 2.8% of the phage adsorbed in 10 min at 60 C, the normal incubation temperature that was used. T\u03c63 was not infective to four other thermophilic strains or to two mesophilic strains of bacteria.",
        "pmcid": "PMC375290",
        "issn": "0022-538X",
        "publisher": "American Society for Microbiology",
        "publication": "Journal of Virology",
        "publication_date": "1967-06",
        "series_number": "3",
        "volume": "1",
        "issue": "3",
        "pages": "610-616"
    }
]