[ { "id": "authors:fb484-1f714", "collection": "authors", "collection_id": "fb484-1f714", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:DINmcb93", "type": "article", "title": "Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis", "author": [ { "family_name": "Ding", "given_name": "Dali", "clpid": "Ding-Dali" }, { "family_name": "Parkhurst", "given_name": "Susan M.", "clpid": "Parkhurst-S-M" }, { "family_name": "Halsell", "given_name": "Susan R.", "clpid": "Halsell-S-R" }, { "family_name": "Lipshitz", "given_name": "Howard D.", "clpid": "Lipshitz-H-D" } ], "abstract": "Hsp83 is the Drosophila homolog of the mammalian Hsp90 family of regulatory molecular chaperones. We show that maternally synthesized Hsp83 transcripts are localized to the posterior pole of the early Drosophila embryo by a novel mechanism involving a combination of generalized RNA degradation and local protection at the posterior. This protection of Hsp83 RNA occurs in wild-type embryos and embryos produced by females carrying the maternal effect mutations nanos and pumilio, which eliminate components of the posterior polar plasm without disrupting polar granule integrity. In contrast, Hsp83 RNA is not protected at the posterior pole of embryos produced by females carrying maternal mutations that disrupt the posterior polar plasm and the polar granules--cappuccino, oskar, spire, staufen, tudor, valois, and vasa. Mislocalization of oskar RNA to the anterior pole, which has been shown to result in induction of germ cells at the anterior, leads to anterior protection of maternal Hsp83 RNA. These results suggest that Hsp83 RNA is a component of the posterior polar plasm that might be associated with polar granules. In addition, we show that zygotic expression of Hsp83 commences in the anterior third of the embryo at the syncytial blastoderm stage and is regulated by the anterior morphogen, bicoid. We consider the possible developmental significance of this complex control of Hsp83 transcript distribution.", "pmcid": "PMC359859", "issn": "0270-7306", "publisher": "American Society for Microbiology", "publication": "Molecular and Cellular Biology", "publication_date": "1993-06", "series_number": "6", "volume": "13", "issue": "6", "pages": "3773-3781" }, { "id": "authors:vkv67-jjr62", "collection": "authors", "collection_id": "vkv67-jjr62", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141215-134934028", "type": "article", "title": "Different genetic requirements for anterior RNA localization revealed by the distribution of Adducin-like transcripts during Drosophila oogenesis", "author": [ { "family_name": "Ding", "given_name": "Dali", "clpid": "Ding-Dali" }, { "family_name": "Parkhurst", "given_name": "Susan M.", "clpid": "Parkhurst-S-M" }, { "family_name": "Lipshitz", "given_name": "Howard D.", "clpid": "Lipshitz-H-D" } ], "abstract": "The proteins encoded by polar-localized mRNAs play an important role in cell fate specification along the anteroposterior axis of the Drosophila embryo. The only maternally synthesized mRNA known previously to be localized to the anterior cortex of both the oocyte and the early embryo is the bicoid mRNA whose localization is required to generate a homeodomain protein gradient that specifies position along the anteroposterior embryonic axis. We have identified and characterized a second mRNA that is localized to the anterior pole of the oocyte and early embryo. This mRNA encodes a Drosophila homolog of mammalian adducin, a membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. A comparison of the spatial distribution of bicoid and Adducin-like transcripts in the maternal-effect RNA-localization mutants exuperantia, swallow, and staufen indicates different genetic requirements for proper localization of these two mRNAs to the anterior pole of the oocyte and early embryo.", "doi": "10.1073/pnas.90.6.2512", "pmcid": "PMC46118", "issn": "0027-8424", "publisher": "National Academy of Sciences", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "publication_date": "1993-03-15", "series_number": "6", "volume": "90", "issue": "6", "pages": "2512-2516" }, { "id": "authors:a9zph-fq263", "collection": "authors", "collection_id": "a9zph-fq263", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20161214-113341284", "type": "article", "title": "Phage lambda cDNA cloning vectors for subtractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning", "author": [ { "family_name": "Palazzolo", "given_name": "Michael J.", "clpid": "Palazzolo-M-J" }, { "family_name": "Hamilton", "given_name": "Bruce A.", "clpid": "Hamilton-B-A" }, { "family_name": "Ding", "given_name": "Dali", "clpid": "Ding-Dali" }, { "family_name": "Martin", "given_name": "Christopher H.", "clpid": "Martin-Christopher-H" }, { "family_name": "Mead", "given_name": "David A.", "clpid": "Mead-D-A" }, { "family_name": "Mierendorf", "given_name": "Robert C.", "clpid": "Mierendorf-R-C" }, { "family_name": "Vijay Raghavan", "given_name": "K.", "clpid": "VijayRaghavan-K" }, { "family_name": "Meyerowitz", "given_name": "Elliot M.", "orcid": "0000-0003-4798-5153", "clpid": "Meyerowitz-E-M" }, { "family_name": "Lipshitz", "given_name": "Howard D.", "clpid": "Lipshitz-H-D" } ], "abstract": "We describe the construction and use of two classes of cDNA cloning vectors. The first class comprises the \u039bEXLX(+) and \u03bbEXLX(\u2212) vectors that can be used for the expression in Escherichia coli of protein encoded by cDNA inserts is achived by the fusion of cDNA open reading frames to the T7 gene 10 promoter and protein-coding sequences. The second class, the \u03bbSHLX vectors, allows the generation of large amounts of single-stranded DNA or synthetic cRNA that can be used in subtractive hybridization procedures. Both classes of vectors are designed to allow directional cDNA cloning with non-enzymatic protection of internal restriction sites. In addition, they are designed to facilitate conversion from phage \u03bb to plasmid clones using a genetic method based on the bacteriophage P1 site-specific recombination system; we refer to this as automatic Cre-loxP plasmid subcloning. The phage \u03bb arms, \u03bbLOX, used in the construction of these vectors have unique restriction sites positioned between the two loxP sites. Insertion of specialized plasmid between these items sites will convert it into a phage \u03bb cDNA cloning vector with automatic plasmid subcloning capability.", "doi": "10.1016/0378-1119(90)90056-W", "issn": "0378-1119", "publisher": "Elsevier", "publication": "Gene", "publication_date": "1990-03-30", "series_number": "1", "volume": "88", "issue": "1", "pages": "25-36" } ]