[
    {
        "id": "authors:a5bpa-jz653",
        "collection": "authors",
        "collection_id": "a5bpa-jz653",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200421-083140254",
        "type": "article",
        "title": "A tribute to Frances Arnold",
        "author": [
            {
                "family_name": "Chen",
                "given_name": "Wilfred",
                "clpid": "Chen-Wilfred"
            },
            {
                "family_name": "Collins",
                "given_name": "Cynthia",
                "clpid": "Collins-C-H"
            },
            {
                "family_name": "Cirino",
                "given_name": "Patrick",
                "orcid": "0000-0002-7245-6205",
                "clpid": "Cirino-P-C"
            },
            {
                "family_name": "Harold",
                "given_name": "Michael P.",
                "clpid": "Harold-M-P"
            }
        ],
        "abstract": "[no abstract]",
        "doi": "10.1002/aic.16923",
        "issn": "0001-1541",
        "publisher": "Wiley",
        "publication": "AIChE Journal",
        "publication_date": "2020-03",
        "series_number": "3",
        "volume": "66",
        "issue": "3",
        "pages": "Art. No. e16923"
    },
    {
        "id": "authors:mn1vz-zkw24",
        "collection": "authors",
        "collection_id": "mn1vz-zkw24",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180313-140924453",
        "type": "article",
        "title": "Process characterization of a novel cross-regulation system for cloned protein production in Escherichia coli",
        "author": [
            {
                "family_name": "Chen",
                "given_name": "Wilfred",
                "clpid": "Chen-Wilfred"
            },
            {
                "family_name": "Kallio",
                "given_name": "Pauli T.",
                "clpid": "Kallio-P-T"
            },
            {
                "family_name": "Bailey",
                "given_name": "James E.",
                "clpid": "Bailey-J-E"
            }
        ],
        "abstract": "A novel cross-regulation expression system has been shown previously to be very\neffective for regulated recombinant protein production. Earlier studies established\nthat this system offers better control of basal expression and higher maximal induced\nexpression than more traditional vectors. Using production of cloned chloramphemcol\nacetyltransferase (CAT) as a model system, several factors determining performance\nof this system were examined. Specifically, the effects of varying induction\ntimes and inducer (IPTG) concentrations on cell growth and the rate of CAT product10n\nwere examined. The CAT expression was maximally induced with at least 0.5 mM\nIPTG added at the midexponential growth phase. Specific CAT content (on a total\nprotein basis) was correlated with the CAT mRNA level. CAT message levels were\nminimal preinduction and far above background postinduction, consistent with pr10r\nsimulation results. Cessation of CAT accumulation as the culture entered the\nstationary phase coincided with a corresponding 10-fold decrease in the level of CAT\nmRNA which was likely caused by an increased mRNA degradation rate. Maintenance\nof significant CAT message levels with a concomitant 2-fold increase in CAT\naccumulation was achieved by extending cell growth in a fed-batch process.",
        "issn": "8756-7938",
        "publisher": "American Chemical Society",
        "publication": "Biotechnology Progress",
        "publication_date": "1995-07-01",
        "series_number": "4",
        "volume": "11",
        "issue": "4",
        "pages": "397-402"
    },
    {
        "id": "authors:k4t7g-zrr33",
        "collection": "authors",
        "collection_id": "k4t7g-zrr33",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180322-152335403",
        "type": "article",
        "title": "Intracellular expression of Vitreoscilla hemoglobin alters the aerobic metabolism of Saccharomyces cerevisiae",
        "author": [
            {
                "family_name": "Chen",
                "given_name": "Wilfred",
                "clpid": "Chen-Wilfred"
            },
            {
                "family_name": "Hughes",
                "given_name": "Dallas E.",
                "clpid": "Hughes-D-E"
            },
            {
                "family_name": "Bailey",
                "given_name": "James E.",
                "clpid": "Bailey-J-E"
            }
        ],
        "abstract": "Vitreoscilla hemoglobin (VHb) has been expressed in Saccharomyces cerevisiae, and its influence on yeast aerobic metabolism has been investigated. New expression vectors were constructed to express VHb constitutively under the control of the ADH\u20101 promoter. The presence of VHb was shown by Western blot analysis. VHb has been shown to localize predominantly in the cytoplasm. Batch fermentation results indicated that the wild\u2010type strain expressing VHb exhibited a shift in the carbon flux toward ethanol production, with no significant alteration in the specific growth rate. This effect was not observed if cells were grown under respiration inhibition, indicating that the metabolic effect of VHb is likely linked to respiration. Expression of VHb in the adh\u00b0 strain MC65\u20102A, which produces ethanol only via a respiration\u2010coupled pathway, revealed that ethanol production was decreased and cells reached a higher final cell density in a culture of the VHb\u2010expressing strain. Growth enhancement due to expression of VHb was observed only during the final stage of culture growth when the acetaldehyde produced during the first growth phase was used as a substrate. This metabolic effect of intracellular VHb was seen more clearly in an acetaldehyde fed\u2010batch fermentation in which VHb\u2010expressing cells grew to at least 3\u2010fold higher final cell density. These results suggest that the action of VHb is likely linked to electron transfer.",
        "doi": "10.1021/bp00027a011",
        "issn": "8756-7938",
        "publisher": "American Chemical Society",
        "publication": "Biotechnology Progress",
        "publication_date": "1994-05",
        "series_number": "3",
        "volume": "10",
        "issue": "3",
        "pages": "308-313"
    }
]