[ { "id": "authors:j3w9p-mpa87", "collection": "authors", "collection_id": "j3w9p-mpa87", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170623-125222628", "type": "article", "title": "Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish", "author": [ { "family_name": "Chen", "given_name": "Shijia", "clpid": "Chen-Shijia" }, { "family_name": "Reichert", "given_name": "Sabine", "clpid": "Reichert-Sabine" }, { "family_name": "Singh", "given_name": "Chanpreet", "orcid": "0000-0002-2697-3033", "clpid": "Singh-Chanpreet" }, { "family_name": "Oikonomou", "given_name": "Grigorios", "orcid": "0000-0001-6797-7375", "clpid": "Oikonomou-Grigorios" }, { "family_name": "Rihel", "given_name": "Jason", "orcid": "0000-0003-4067-2066", "clpid": "Rihel-Jason" }, { "family_name": "Prober", "given_name": "David A.", "orcid": "0000-0002-7371-4675", "clpid": "Prober-D-A" } ], "abstract": "Light affects sleep and wake behaviors by providing an indirect cue that entrains circadian rhythms and also by inducing a direct and rapid regulation of behavior. While circadian entrainment by light is well characterized at the molecular level, mechanisms that underlie the direct effect of light on behavior are largely unknown. In zebrafish, a diurnal vertebrate, we found that both overexpression and mutation of the neuropeptide prokineticin 2 (Prok2) affect sleep and wake behaviors in a light-dependent but circadian-independent manner. In light, Prok2 overexpression increases sleep and induces expression of galanin (galn), a hypothalamic sleep-inducing peptide. We also found that light-dependent, Prok2-induced sedation requires prokineticin receptor 2 (prokr2) and is strongly suppressed in galn mutants. These results suggest that Prok2 antagonizes the direct wake-promoting effect of light in zebrafish, in part through the induction of galn expression in the hypothalamus.", "doi": "10.1016/j.neuron.2017.06.001", "pmcid": "PMC5653285", "issn": "0896-6273", "publisher": "Cell Press", "publication": "Neuron", "publication_date": "2017-07-05", "series_number": "1", "volume": "95", "issue": "1", "pages": "153-168" }, { "id": "authors:9x4vy-xj986", "collection": "authors", "collection_id": "9x4vy-xj986", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160218-075222867", "type": "article", "title": "A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States", "author": [ { "family_name": "Chiu", "given_name": "Cindy N.", "clpid": "Chiu-Cindy-N" }, { "family_name": "Rihel", "given_name": "Jason", "orcid": "0000-0003-4067-2066", "clpid": "Rihel-Jason" }, { "family_name": "Lee", "given_name": "Daniel A.", "orcid": "0000-0001-7411-2740", "clpid": "Lee-Daniel-A" }, { "family_name": "Singh", "given_name": "Chanpreet", "orcid": "0000-0002-2697-3033", "clpid": "Singh-Chanpreet" }, { "family_name": "Mosser", "given_name": "Eric A.", "clpid": "Mosser-Eric-A" }, { "family_name": "Chen", "given_name": "Shijia", "clpid": "Chen-Shijia" }, { "family_name": "Sapin", "given_name": "Viveca", "clpid": "Sapin-Viveca" }, { "family_name": "Pham", "given_name": "Uyen", "clpid": "Pham-Uyen" }, { "family_name": "Engle", "given_name": "Jae", "clpid": "Engle-Jae" }, { "family_name": "Niles", "given_name": "Brett J.", "clpid": "Niles-Brett-J" }, { "family_name": "Montz", "given_name": "Christin J.", "clpid": "Montz-Christin-J" }, { "family_name": "Chakravarthy", "given_name": "Sridhara", "clpid": "Chakravarthy-Sridhara" }, { "family_name": "Zimmerman", "given_name": "Steven", "clpid": "Zimmerman-Steven" }, { "family_name": "Salehi-Ashtiani", "given_name": "Kourosh", "orcid": "0000-0002-6521-5243", "clpid": "Salehi-Ashtiani-Kourosh" }, { "family_name": "Vidal", "given_name": "Marc", "orcid": "0000-0003-3391-5410", "clpid": "Vidal-Marc" }, { "family_name": "Schier", "given_name": "Alexander F.", "orcid": "0000-0002-5317-494X", "clpid": "Schier-Alexander-F" }, { "family_name": "Prober", "given_name": "David A.", "orcid": "0000-0002-7371-4675", "clpid": "Prober-D-A" } ], "abstract": "Neuromodulation of arousal states ensures that an animal appropriately responds to its environment and engages in behaviors necessary for survival. However, the molecular and circuit properties underlying neuromodulation of arousal states such as sleep and wakefulness remain unclear. To tackle this challenge in a systematic and unbiased manner, we performed a genetic overexpression screen to identify genes that affect larval zebrafish arousal. We found that the neuropeptide neuromedin U (Nmu) promotes hyperactivity and inhibits sleep in zebrafish larvae, whereas nmu mutant animals are hypoactive. We show that Nmu-induced arousal requires Nmu receptor 2 and signaling via corticotropin releasing hormone (Crh) receptor 1. In contrast to previously proposed models, we find that Nmu does not promote arousal via the hypothalamic-pituitary-adrenal axis, but rather probably acts via brainstem crh-expressing neurons. These results reveal an unexpected functional and anatomical interface between the Nmu system and brainstem arousal systems that represents a novel wake-promoting pathway.", "doi": "10.1016/j.neuron.2016.01.007", "pmcid": "PMC4851465", "issn": "0896-6273", "publisher": "Cell Press", "publication": "Neuron", "publication_date": "2016-02-17", "series_number": "4", "volume": "89", "issue": "4", "pages": "842-856" }, { "id": "authors:qkreg-5ad57", "collection": "authors", "collection_id": "qkreg-5ad57", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151030-151749046", "type": "article", "title": "TRP channel mediated neuronal activation and ablation in freely behaving zebrafish", "author": [ { "family_name": "Chen", "given_name": "Shijia", "clpid": "Chen-Shijia" }, { "family_name": "Chiu", "given_name": "Cindy N.", "clpid": "Chiu-Cindy-N" }, { "family_name": "McArthur", "given_name": "Kimberly L.", "clpid": "McArthur-K-L" }, { "family_name": "Fetcho", "given_name": "Joseph R.", "clpid": "Fetcho-J-R" }, { "family_name": "Prober", "given_name": "David A.", "orcid": "0000-0002-7371-4675", "clpid": "Prober-D-A" } ], "abstract": "The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.", "doi": "10.1038/nmeth.3691", "pmcid": "PMC4851460", "issn": "1548-7091", "publisher": "Nature Publishing Group", "publication": "Nature Methods", "publication_date": "2016-02", "series_number": "2", "volume": "13", "issue": "2", "pages": "147-150" }, { "id": "authors:0829p-9k457", "collection": "authors", "collection_id": "0829p-9k457", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130605-105912475", "type": "article", "title": "A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly", "author": [ { "family_name": "Chen", "given_name": "Shijia", "clpid": "Chen-Shijia" }, { "family_name": "Oikonomou", "given_name": "Grigorios", "orcid": "0000-0001-6797-7375", "clpid": "Oikonomou-G" }, { "family_name": "Chiu", "given_name": "Cindy N.", "clpid": "Chiu-Cindy-N" }, { "family_name": "Niles", "given_name": "Brett J.", "clpid": "Niles-B-J" }, { "family_name": "Liu", "given_name": "Justin", "orcid": "0000-0002-5338-6491", "clpid": "Liu-Justin" }, { "family_name": "Lee", "given_name": "Daniel A.", "orcid": "0000-0001-7411-2740", "clpid": "Lee-Daniel-A" }, { "family_name": "Antoshechkin", "given_name": "Igor", "orcid": "0000-0002-9934-3040", "clpid": "Antoshechkin-I-A" }, { "family_name": "Prober", "given_name": "David A.", "orcid": "0000-0002-7371-4675", "clpid": "Prober-D-A" } ], "abstract": "Zinc-finger nucleases (ZFNs) and TAL effector nucleases\n(TALENs) have been shown to induce\ntargeted mutations, but they have not been extensively\ntested in any animal model. Here, we describe\na large-scale comparison of ZFN and TALEN\nmutagenicity in zebrafish. Using deep sequencing,\nwe found that TALENs are significantly more likely\nto be mutagenic and induce an average of 10-fold\nmore mutations than ZFNs. We observed a strong\ncorrelation between somatic and germ-line mutagenicity,\nand identified germ line mutations using\nZFNs whose somatic mutations rates are well\nbelow the commonly used threshold of 1%. Guidelines\nthat have previously been proposed to predict\noptimal ZFN and TALEN target sites did not predict\nmutagenicity in vivo. However, we observed a significant\nnegative correlation between TALEN mutagenicity\nand the number of CpG repeats in TALEN\ntarget sites, suggesting that target site methylation\nmay explain the poor mutagenicity of some TALENs\nin vivo. The higher mutation rates and ability to\ntarget essentially any sequence make TALENs the\nsuperior technology for targeted mutagenesis in\nzebrafish, and likely other animal models.", "doi": "10.1093/nar/gks1356", "pmcid": "PMC3575824", "issn": "0305-1048", "publisher": "Oxford University Press", "publication": "Nucleic Acids Research", "publication_date": "2013-02", "series_number": "4", "volume": "41", "issue": "4", "pages": "2769-2778" } ]