[
    {
        "id": "thesis:7643",
        "collection": "thesis",
        "collection_id": "7643",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:04292013-110424232",
        "primary_object_url": {
            "basename": "Chamberlin_hm_1994.pdf",
            "content": "final",
            "filesize": 50428743,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/7643/1/Chamberlin_hm_1994.pdf",
            "version": "v3.0.0"
        },
        "type": "thesis",
        "title": "Cell fate specification during Caenorhabditis elegans male tail development",
        "author": [
            {
                "family_name": "Chamberlin",
                "given_name": "Helen Marie",
                "clpid": "Chamberlin-H-M"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Sternberg",
                "given_name": "Paul W.",
                "clpid": "Sternberg-P-W"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Davidson",
                "given_name": "Eric H.",
                "clpid": "Davidson-E-H"
            },
            {
                "family_name": "Fraser",
                "given_name": "Scott E.",
                "clpid": "Fraser-S-E"
            },
            {
                "family_name": "Lewis",
                "given_name": "Edward B.",
                "clpid": "Lewis-E-B"
            },
            {
                "family_name": "Lipshitz",
                "given_name": "Howard D.",
                "clpid": "Lipshitz-H-D"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "<p>The cells of the specialized mating structures of the nematode\r\nCaenorhabditis elegans adult male tail develop from sex-specific divisions of\r\npostembryonic blast cells. One male-specific blast cell, B, is the precursor to\r\nall the cells of the copulatory spicules. Both cell interactions and autonomous\r\nfate specification mechanisms are utilized in the B lineage to specify fate.</p>\r\n\r\n<p>During development the anterior daughter of B, B.a, generates four\r\ndistinct pairs of cells. Cell ablation experiments indicate that the cells of\r\neach pair respond to positional cues provided by other male-specific blast\r\ncells. F and U promote anterior fates, Y.p promotes some posterior fates, and\r\nthe B.a progeny promote posterior fates. The cells within each pair may also\r\ninteract.</p>\r\n\r\n<p>The lin-3/let-23 signalling pathway, identified for its function in C.\r\nelegans hermaphrodite vulval induction, mediates the signal from F and U.\r\nReduction-of-function mutations in lin-3 (EGF-like signal), let-23 (receptor),\r\nsem-5 (adaptor), let-60 (ras), or lin-45 (raf) disrupt the fates of the anterior\r\ncells, and mimic F and U ablation. In addition, ectopically expressed lin-3\r\ndisrupts the fates of the posterior cells, and can promote anterior fates even\r\nin the absence of F and U.</p>\r\n\r\n<p>A genetic screen of over 9000 mutagenized gametes recovered 22\r\nmutations in 20 loci that disrupt fate specification in male tail lineages.\r\nSeven of these mutations may represent new genes that play a role in male\r\ntail development.</p>\r\n\r\n<p>The first division of the B cell is asymmetric. The gene vab-3 is\r\nrequired for specification of B.a fates, and it may represent a factor whose\r\nactivity is localized to the B.a cell via the gene lin-17. lin-17 acts both at the\r\nfirst division of the B cell and at specific other cell divisions in the lineage.</p>\r\n\r\n",
        "doi": "10.7907/nmjn-dx76",
        "publication_date": "1994",
        "thesis_type": "phd",
        "thesis_year": "1994"
    }
]