[ { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4fnqx-tnb67", "eprint_status": "archive", "datestamp": "2023-12-21 20:19:41", "lastmod": "2024-01-09 22:23:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Fasse-Aria-J", "name": { "family": "Fasse", "given": "Aria J." }, "orcid": "0009-0009-2894-9908" }, { "id": "Camacho-Avila-Alexis", "name": { "family": "Camacho-Avila", "given": "Alexis" } }, { "id": "Grabylnikov-Ilya", "name": { "family": "Grabylnikov", "given": "Ilya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "SMPD3 expression is spatially regulated in the developing embryo by SOXE factors", "ispublished": "pub", "full_text_status": "public", "keywords": "Cell Biology; Developmental Biology; Molecular Biology", "note": "
\u00a9 2023 Elsevier.
\n\nWe would like to thank Drs. Tatjana Sauka-Spengler, Ruth Williams, and Ivan Candido Ferreira for sharing data and helpful discussion on enhancer identification and dissection. We'd also like to thank the Caltech Biological Imaging Facility, supported by the Caltech Beckman Institute and the Arnold and Mabel Beckman Foundation, for confocal microscopy support. Funding for this work comes from the NIH grants K99/R00 DE029240 to M.L.P., R01 DE027538 to M.E.B., and a Traveling Fellowship DEVTF1908262 provided by the Company of Biologists to M.L.P.
\n\nConceptualization: M.L.P.
Experiment design: M.L.P.
Experimentation: M.L.P., A.J.F., A.C.A., and I.G.
Data analysis: M.L.P. and A.J.F.
Data interpretation: M.L.P. and M.E.B.
Manuscript preparation: M.L.P.
Manuscript editing: M.E.B.
\n\nWe have provided all raw source data and analysis code in our GitHub repository, linked within the manuscript.
\n\nThe authors declare no conflicts of interest.
", "abstract": "During epithelial-to-mesenchymal transition (EMT), significant rearrangements occur in plasma membrane protein and lipid content that are important for membrane function and acquisition of cell motility. To gain insight into how neural crest cells regulate their lipid content at the transcriptional level during EMT, here we identify critical enhancer sequences that regulate the expression of SMPD3, a gene responsible for sphingomyelin hydrolysis to produce ceramide and necessary for neural crest EMT. We uncovered three enhancer regions within the first intron of the SMPD3 locus that drive reporter expression in distinct spatial and temporal domains, together collectively recapitulating the expression domains of endogenous SMPD3 within the ectodermal lineages. We further dissected one enhancer that is specifically active in the migrating neural crest. By mutating putative transcriptional input sites or knocking down upstream regulators, we find that the SOXE-family transcription factors SOX9 and SOX10 regulate the expression of SMPD3 in migrating neural crest cells. Further, ChIP-seq and nascent transcription analysis reveal that SOX10 directly regulates expression of an SMPD3 enhancer specific to migratory neural crest cells. Together these results shed light on how core components of developmental gene regulatory networks interact with metabolic effector genes to control changes in membrane lipid content.
", "date": "2024-02", "date_type": "published", "publication": "Developmental Biology", "volume": "506", "publisher": "Elsevier", "pagerange": "31-41", "issn": "0012-1606", "official_url": "https://authors.library.caltech.edu/records/4fnqx-tnb67", "funders": { "items": [ { "grant_number": "Caltech Beckman Institute" }, {}, { "grant_number": "K99/R00 DE029240" }, { "grant_number": "R01 DE027538" }, { "grant_number": "DEVTF1908262" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" }, { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" } ] }, "doi": "10.1016/j.ydbio.2023.11.011", "primary_object": { "basename": "1-s2.0-S0012160623002014-mmc1.docx", "url": "https://authors.library.caltech.edu/records/4fnqx-tnb67/files/1-s2.0-S0012160623002014-mmc1.docx" }, "resource_type": "article", "pub_year": "2024", "author_list": "Piacentino, Michael L.; Fasse, Aria J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bpha3-br295", "eprint_status": "archive", "datestamp": "2023-11-09 19:18:27", "lastmod": "2024-01-09 22:24:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Jacobs-Li-Jessica", "name": { "family": "Jacobs-Li", "given": "Jessica" }, "orcid": "0000-0003-1339-3555" }, { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Li-Can", "name": { "family": "Li", "given": "Can" }, "orcid": "0000-0002-9301-7850" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous system", "ispublished": "pub", "full_text_status": "public", "keywords": "General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Medicine; General Neuroscience", "note": "\u00a9 2023, Jacobs-Li, Tang et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
\n\nThis work was supported by 1R01DK133480 to MEB and F31 HD111287 to JLL We thank Drs. Igor Antoshechkin and Vijaya Kumar and the Millard and Muriel Jacobs Genetics and Genomics Laboratory at California Institute of Technology for their guidance and support in bulk RNA-sequencing. We thank Jamie Tijerina and Rochelle Diamond from the Beckman Institute Flow Cytometry Facility for their help with the FACS. We thank Dr. Sisi Chen, Jeff Park, Prof. Matt Thomson, and SPEC at Caltech for their dedicated support in optimization and guidance in single-cell RNA-sequencing. We thank Dr. Fan Gao and Bioinformatics Resource Center in the Beckman Institute at Caltech for guiding us through single-cell transcriptomic analysis. We appreciate the help from Prof. Carlos Lois for kindly sharing equipment with us to perform RIA concentration. We thank Dr. Michael L Piacentino, Dr. Erica J Hutchins, and Prof. Angelike Stathopoulos for the helpful discussion on the manuscript.
\n\nJessica Jacobs-Li, Data curation, Formal analysis, Validation, Writing - review and editing; Weiyi Tang, Conceptualization, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft, Writing - review and editing; Can Li, Formal analysis, Methodology; Marianne E Bronner, Conceptualization, Resources, Funding acquisition, Writing - original draft, Project administration, Writing - review and editing
\n\nMarianne E Bronner: Senior editor, eLife. The other authors declare that no competing interests exist.
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
", "abstract": "During development, much of the enteric nervous system (ENS) arises from the vagal neural crest that emerges from the caudal hindbrain and colonizes the entire gastrointestinal tract. However, a second ENS contribution comes from the sacral neural crest that arises in the caudal neural tube and populates the post-umbilical gut. By coupling single-cell transcriptomics with axial-level-specific lineage tracing in avian embryos, we compared the contributions of embryonic vagal and sacral neural crest cells to the chick ENS and the associated peripheral ganglia (Nerve of Remak and pelvic plexuses). At embryonic day (E) 10, the two neural crest populations form overlapping subsets of neuronal and glia cell types. Surprisingly, the post-umbilical vagal neural crest much more closely resembles the sacral neural crest than the pre-umbilical vagal neural crest. However, some differences in cluster types were noted between vagal and sacral derived cells. Notably, RNA trajectory analysis suggests that the vagal neural crest maintains a neuronal/glial progenitor pool, whereas this cluster is depleted in the E10 sacral neural crest which instead has numerous enteric glia. The present findings reveal sacral neural crest contributions to the hindgut and associated peripheral ganglia and highlight the potential influence of the local environment and/or developmental timing in differentiation of neural crest-derived cells in the developing ENS.", "date": "2023-10-25", "date_type": "published", "publication": "eLife", "volume": "12", "publisher": "eLife", "pagerange": "e79156", "issn": "2050-084X", "official_url": "https://authors.library.caltech.edu/records/bpha3-br295", "funders": { "items": [ { "grant_number": "R01DK13348" }, { "grant_number": "F31HD11128" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" }, { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.7554/elife.79156", "pmcid": "PMC10627514", "primary_object": { "basename": "elife-79156-v2.pdf", "url": "https://authors.library.caltech.edu/records/bpha3-br295/files/elife-79156-v2.pdf" }, "resource_type": "article", "pub_year": "2023", "author_list": "Jacobs-Li, Jessica; Tang, Weiyi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/22qyc-gw073", "eprint_status": "archive", "datestamp": "2023-11-02 23:21:59", "lastmod": "2024-01-09 22:23:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lamanna-Francesco", "name": { "family": "Lamanna", "given": "Francesco" }, "orcid": "0000-0002-0447-759X" }, { "id": "Hervas-Sotomayor-Francisca", "name": { "family": "Hervas-Sotomayor", "given": "Francisca" }, "orcid": "0009-0007-0965-2493" }, { "id": "Oel-A-Phillip", "name": { "family": "Oel", "given": "A. Phillip" }, "orcid": "0000-0003-4062-0987" }, { "id": "Jandzik-David", "name": { "family": "Jandzik", "given": "David" }, "orcid": "0000-0002-4239-0014" }, { "id": "Sobrido-Came\u00e1n-Daniel", "name": { "family": "Sobrido-Came\u00e1n", "given": "Daniel" }, "orcid": "0000-0001-8239-2965" }, { "id": "Santos-Dur\u00e1n-Gabriel-Nicol\u00e1s", "name": { "family": "Santos-Dur\u00e1n", "given": "Gabriel N." }, "orcid": "0000-0002-2415-0332" }, { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Stundl-Jan", "name": { "family": "Stundl", "given": "Jan" }, "orcid": "0000-0002-3740-3378" }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Br\u00fcning-Thoomke", "name": { "family": "Br\u00fcning", "given": "Thoomke" } }, { "id": "M\u00f6\u00dfinger-Katharina", "name": { "family": "M\u00f6\u00dfinger", "given": "Katharina" } }, { "id": "Schmidt-Julia", "name": { "family": "Schmidt", "given": "Julia" } }, { "id": "Schneider-Celine", "name": { "family": "Schneider", "given": "Celine" } }, { "id": "Sepp-Mari", "name": { "family": "Sepp", "given": "Mari" }, "orcid": "0000-0003-1733-8385" }, { "id": "Murat-Florent", "name": { "family": "Murat", "given": "Florent" }, "orcid": "0000-0003-2116-2511" }, { "id": "Smith-Jeramiah-J", "name": { "family": "Smith", "given": "Jeramiah J." }, "orcid": "0000-0001-5333-5531" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Rodicio-Mar\u00eda-Celina", "name": { "family": "Rodicio", "given": "Mar\u00eda Celina" }, "orcid": "0000-0002-7267-3285" }, { "id": "Barreiro-Iglesias-Ant\u00f3n", "name": { "family": "Barreiro-Iglesias", "given": "Ant\u00f3n" }, "orcid": "0000-0002-7507-080X" }, { "id": "Medeiros-Daniel-M", "name": { "family": "Medeiros", "given": "Daniel M." }, "orcid": "0000-0002-8182-6028" }, { "id": "Arendt-Detlev", "name": { "family": "Arendt", "given": "Detlev" }, "orcid": "0000-0001-7833-050X" }, { "id": "Kaessmann-Henrik", "name": { "family": "Kaessmann", "given": "Henrik" }, "orcid": "0000-0001-7563-839X" } ] }, "title": "A lamprey neural cell type atlas illuminates the origins of the vertebrate brain", "ispublished": "pub", "full_text_status": "public", "keywords": "Ecology; Ecology, Evolution, Behavior and Systematics", "note": "\u00a9 The Author(s) 2023, corrected publication 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
\n\nWe thank all members of the Kaessmann group for fruitful discussions; Robert Fr\u00f6mel for his help with library sequencing; E. Panzariello, M. Sanchez-Delgado and N. Trost for the brain and animal illustrations; M. Mall for providing temporary lab space; and M. Cardoso-Moreira for discussions and comments on the manuscript. The computations were performed on the Kaessmann lab server (managed by N. Trost) and the bwForCluster from the Heidelberg University Computational Center (supported by the state of Baden-W\u00fcrttemberg through bwHPC and the German Research Foundation, INST 35/1134-1 FUGG). We thank W. Wang of the Northwestern Polytechnical University of Xi'an, China; Y. Zhang of the Institute of Zoology, Chinese Academy of Sciences; and J. Pascual-Anaya from the University of M\u00e1laga, Spain, for granting access to the early draft of the inshore hagfish genome (https://doi.org/10.1101/2023.04.08.536076). This work was supported by grants from the European Research Council (no. 615253, OntoTransEvol), the European Commission (Marie Sk\u0142odowska-Curie Actions ITN: EvoCELL) and the Tschira foundation, which funded the Illumina NextSeq machine used for sequencing. D.M.M. and D.J. were supported by grants from the National Science Foundation (IOS 1656843), the National Institutes of Health/National Institute of Dental and Craniofacial Research (RDE025940) and University of Colorado, Boulder RIO Innovative Seed Grant FY21 (all to D.M.M.). D.J. was also supported by a grant from the Scientific Grant Agency of the Slovak Republic (VEGA 1/0450/21). This project has received funding from the European Research Council under the European Union's Horizon 2020 research and innovation programme (VerteBrain to H.K., grant agreement no. 101019268; and NeuralCellTypeEvo to D.A., grant agreement no. 788921).
\n\nThese authors contributed equally: Francesco Lamanna, Francisca Hervas-Sotomayor.
F.L., F.H.-S. and H.K. conceived and organized the study on the basis of H.K.'s original design. F.L., F.H.-S. and H.K. wrote the manuscript with input from all authors. F.L. performed all analyses and developed the brain atlas app. F.H.-S. established and optimized the tissue dissociation protocol and performed all scRNA-seq and in situ experiments with support from A.P.O., J. Schmidt and C.S. and guidance from M.S. F.L. and F.H.-S. annotated and interpreted the data. T.B. prepared the bulk libraries with guidance from K.M. M.S. established the smRNA-FISH protocol. F.H.-S., A.P.O., D.J., D.S.-C., G.N.S.-D., M.L.M., J. Stundl and S.A.G. collected the samples. A.B.-I., D.M.M., M.E.B. and M.C.R. provided the samples. J.J.S. provided early access to genome assemblies and annotations. A.P.O., M.S., F.M., D.S.-C., A.B.-I., D.M.M. and D.A. provided useful feedback and discussions. H.K. supervised the study and provided funding.
\n\nThe raw and processed bulk and scRNA-seq data have been deposited to ArrayExpress with the accession numbers E-MTAB-11085 (bulk) and E-MTAB-11087 (single cell) (https://www.ebi.ac.uk/arrayexpress/). The genome annotation files and in situ images have been deposited to Zenodo110 (https://doi.org/10.5281/zenodo.5903844). Information about gene expression, cell type annotation and gene orthology relationships across species can be visualized using the online atlas (https://lampreybrain.kaessmannlab.org/).
\n\nAll code underlying the published atlas is available on GitHub (https://github.com/f-lamanna/LampreyBrainAtlas/) and Zenodo111 (https://doi.org/10.5281/zenodo.8113793) together with detailed instructions about its usage.
\n\nThe authors declare no competing interests.
", "abstract": "The vertebrate brain emerged more than ~500 million years ago in common evolutionary ancestors. To systematically trace its cellular and molecular origins, we established a spatially resolved cell type atlas of the entire brain of the sea lamprey\u2014a jawless species whose phylogenetic position affords the reconstruction of ancestral vertebrate traits\u2014based on extensive single-cell RNA-seq and in situ sequencing data. Comparisons of this atlas to neural data from the mouse and other jawed vertebrates unveiled various shared features that enabled the reconstruction of cell types, tissue structures and gene expression programs of the ancestral vertebrate brain. However, our analyses also revealed key tissues and cell types that arose later in evolution. For example, the ancestral brain was probably devoid of cerebellar cell types and oligodendrocytes (myelinating cells); our data suggest that the latter emerged from astrocyte-like evolutionary precursors in the jawed vertebrate lineage. Altogether, our work illuminates the cellular and molecular architecture of the ancestral vertebrate brain and provides a foundation for exploring its diversification during evolution.
", "date": "2023-10", "date_type": "published", "publication": "Nature Ecology & Evolution", "volume": "7", "number": "10", "publisher": "Nature Publishing Group", "pagerange": "1714-1728", "issn": "2397-334X", "official_url": "https://authors.library.caltech.edu/records/22qyc-gw073", "funders": { "items": [ { "grant_number": "INST 35/1134-1 FUGG" }, { "grant_number": "615253" }, {}, { "grant_number": "IOS-1656843" }, { "grant_number": "RDE025940" }, {}, { "grant_number": "101019268" }, { "grant_number": "788921" } ] }, "local_group": { "items": [ { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1038/s41559-023-02170-1", "pmcid": "PMC10555824", "primary_object": { "basename": "41559_2023_2170_Fig12_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig12_ESM.jpg" }, "related_objects": [ { "basename": "41559_2023_2170_Fig16_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig16_ESM.jpg" }, { "basename": "41559_2023_2170_Fig8_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig8_ESM.jpg" }, { "basename": "41559_2023_2170_MOESM5_ESM.zip", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_MOESM5_ESM.zip" }, { "basename": "41559_2023_2170_Fig15_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig15_ESM.jpg" }, { "basename": "41559_2023_2170_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_MOESM1_ESM.pdf" }, { "basename": "s41559-023-02170-1.pdf", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/s41559-023-02170-1.pdf" }, { "basename": "41559_2023_2170_Fig11_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig11_ESM.jpg" }, { "basename": "41559_2023_2170_Fig13_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig13_ESM.jpg" }, { "basename": "41559_2023_2170_Fig14_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig14_ESM.jpg" }, { "basename": "41559_2023_2170_Fig7_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig7_ESM.jpg" }, { "basename": "41559_2023_2170_Fig10_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig10_ESM.jpg" }, { "basename": "41559_2023_2170_Fig9_ESM.jpg", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_Fig9_ESM.jpg" }, { "basename": "41559_2023_2170_MOESM4_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_MOESM4_ESM.xlsx" }, { "basename": "41559_2023_2170_MOESM6_ESM.zip", "url": "https://authors.library.caltech.edu/records/22qyc-gw073/files/41559_2023_2170_MOESM6_ESM.zip" } ], "resource_type": "article", "pub_year": "2023", "author_list": "Lamanna, Francesco; Hervas-Sotomayor, Francisca; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/20qzy-05s33", "eprint_id": 122434, "eprint_status": "archive", "datestamp": "2023-08-22 21:27:38", "lastmod": "2024-01-18 17:33:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stundl-Jan", "name": { "family": "Stundl", "given": "Jan" }, "orcid": "0000-0002-3740-3378" }, { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Chen-Donglei", "name": { "family": "Chen", "given": "Donglei" }, "orcid": "0000-0002-0471-8162" }, { "id": "Raja-Desingu-Ayyappa", "name": { "family": "Raja", "given": "Desingu Ayyappa" }, "orcid": "0000-0002-0173-0465" }, { "id": "Fran\u011bk-Roman", "name": { "family": "Fran\u011bk", "given": "Roman" }, "orcid": "0000-0002-3464-1872" }, { "id": "Pospisilova-Anna", "name": { "family": "Pospisilova", "given": "Anna" }, "orcid": "0000-0002-8252-0709" }, { "id": "P\u0161eni\u010dka-Martin", "name": { "family": "P\u0161eni\u010dka", "given": "Martin" }, "orcid": "0000-0002-3808-7856" }, { "id": "Metscher-Brian-D", "name": { "family": "Metscher", "given": "Brian D." }, "orcid": "0000-0002-6514-4406" }, { "id": "Braasch-Ingo", "name": { "family": "Braasch", "given": "Ingo" }, "orcid": "0000-0003-4766-611X" }, { "id": "Haitina-Tatjana", "name": { "family": "Haitina", "given": "Tatjana" }, "orcid": "0000-0002-8754-5534" }, { "id": "Cerny-Robert", "name": { "family": "Cerny", "given": "Robert" }, "orcid": "0000-0002-0022-0199" }, { "id": "Ahlberg-Per-E", "name": { "family": "Ahlberg", "given": "Per E." }, "orcid": "0000-0001-9054-2900" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ancient vertebrate dermal armor evolved from trunk neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Multidisciplinary", "note": "\u00a9 2023 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). \n\nThe project has received funding from the European Union's Horizon 2020 research and innovation program under Marie Sklodowska-Curie grant agreement No. 897949 (to J.S.) and from National Institutes of Health grant R35NS111564 to (M.E.B). D.C. and P.E.A. were supported by a Wallenberg Scholarship from the Knut & Alice Wallenberg Foundation, awarded to P.E.A. M.L.M. was supported by a fellowship from the Helen Hay Whitney Foundation and by NIH grant 1K99HD100587. J.S., R.F., and M.P. were supported by the Ministry of Education, Youth and Sports of the Czech Republic\u2014project Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370) and the Czech Science Foundation (No. 20-23836S). R.C. was supported by the Czech Science Foundation (No. 19-18634S). Gar work in the Braasch Lab is supported by NSF EDGE FGT grant #2029216. \n\nAuthor Contributions. J.S., P.E.A., and M.E.B. designed research; J.S., M.L.M., D.C., D.A.R., and R.F. performed research; J.S., D.C., A.P., M.P., I.B., T.H., and R.C. contributed new reagents/analytic tools; J.S., M.L.M., D.C., D.A.R., R.F., and B.D.M. analyzed data; and J.S., D.C., P.E.A., and M.E.B. wrote the paper. \n\nData, Materials, and Software Availability. The RNA-seq data reported in this paper have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus database (accession no. GSE235280) (64). \n\nThe authors declare no competing interest.\n\nPublished - pnas.2221120120.pdf
Supplemental Material - pnas.2221120120.sapp.pdf
Supplemental Material - pnas.2221120120.sd01.csv
Supplemental Material - pnas.2221120120.sd02.csv
Supplemental Material - pnas.2221120120.sm01.avi
Supplemental Material - pnas.2221120120.sm02.avi
Supplemental Material - pnas.2221120120.sm03.avi
", "abstract": "Bone is an evolutionary novelty of vertebrates, likely to have first emerged as part of ancestral dermal armor that consisted of osteogenic and odontogenic components. Whether these early vertebrate structures arose from mesoderm or neural crest cells has been a matter of considerable debate. To examine the developmental origin of the bony part of the dermal armor, we have performed in vivo lineage tracing in the sterlet sturgeon, a representative of nonteleost ray-finned fish that has retained an extensive postcranial dermal skeleton. The results definitively show that sterlet trunk neural crest cells give rise to osteoblasts of the scutes. Transcriptional profiling further reveals neural crest gene signature in sterlet scutes as well as bichir scales. Finally, histological and microCT analyses of ray-finned fish dermal armor show that their scales and scutes are formed by bone, dentin, and hypermineralized covering tissues, in various combinations, that resemble those of the first armored vertebrates. Taken together, our results support a primitive skeletogenic role for the neural crest along the entire body axis, that was later progressively restricted to the cranial region during vertebrate evolution. Thus, the neural crest was a crucial evolutionary innovation driving the origin and diversification of dermal armor along the entire body axis.", "date": "2023-07-25", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "120", "number": "30", "publisher": "National Academy of Sciences", "pagerange": "e2221120120", "id_number": "CaltechAUTHORS:20230725-49196000.18", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230725-49196000.18", "funders": { "items": [ { "agency": "Marie Curie Fellowship", "grant_number": "897949" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "Knut and Alice Wallenberg Foundation" }, { "agency": "Helen Hay Whitney Foundation" }, { "agency": "NIH", "grant_number": "1K99HD100587" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "CZ.02.1.01/0.0/0.0/16_025/0007370" }, { "agency": "Czech Science Foundation", "grant_number": "20-23836S" }, { "agency": "Czech Science Foundation", "grant_number": "19-18634S" }, { "agency": "NSF", "grant_number": "IOS-2029216" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" }, { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1073/pnas.2221120120", "pmcid": "PMC10372632", "primary_object": { "basename": "pnas.2221120120.pdf", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.pdf" }, "related_objects": [ { "basename": "pnas.2221120120.sm02.avi", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sm02.avi" }, { "basename": "pnas.2221120120.sm03.avi", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sm03.avi" }, { "basename": "pnas.2221120120.sapp.pdf", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sapp.pdf" }, { "basename": "pnas.2221120120.sd01.csv", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sd01.csv" }, { "basename": "pnas.2221120120.sd02.csv", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sd02.csv" }, { "basename": "pnas.2221120120.sm01.avi", "url": "https://authors.library.caltech.edu/records/20qzy-05s33/files/pnas.2221120120.sm01.avi" } ], "resource_type": "article", "pub_year": "2023", "author_list": "Stundl, Jan; Martik, Megan L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sakps-59297", "eprint_status": "archive", "datestamp": "2024-03-06 18:44:30", "lastmod": "2024-03-06 20:11:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tzung-Keh-Weei", "name": { "family": "Tzung", "given": "Keh-Weei" }, "orcid": "0009-0007-2333-4686" }, { "id": "Lalonde-Robert-L", "name": { "family": "Lalonde", "given": "Robert L." }, "orcid": "0000-0002-1803-0983" }, { "id": "Prummel-Karin-D", "name": { "family": "Prummel", "given": "Karin D." }, "orcid": "0000-0001-6077-6407" }, { "id": "Mahabaleshwar-Harsha", "name": { "family": "Mahabaleshwar", "given": "Harsha" }, "orcid": "0009-0000-7482-5791" }, { "id": "Moran-Hannah-R", "name": { "family": "Moran", "given": "Hannah R." }, "orcid": "0000-0001-8056-5762" }, { "id": "Stundl-Jan", "name": { "family": "Stundl", "given": "Jan" }, "orcid": "0000-0002-3740-3378" }, { "id": "Cass-Amanda-N", "name": { "family": "Cass", "given": "Amanda N." }, "orcid": "0000-0002-8998-9294" }, { "id": "Le-Yao", "name": { "family": "Le", "given": "Yao" }, "orcid": "0000-0003-2935-2993" }, { "id": "Lea-Robert", "name": { "family": "Lea", "given": "Robert" }, "orcid": "0000-0003-0522-3601" }, { "id": "Dorey-Karel", "name": { "family": "Dorey", "given": "Karel" }, "orcid": "0000-0003-0846-5286" }, { "id": "Tomecka-Monika-J", "name": { "family": "Tomecka", "given": "Monika J." } }, { "id": "Zhang-Changqing", "name": { "family": "Zhang", "given": "Changqing" }, "orcid": "0000-0002-2464-6784" }, { "id": "Brombacher-Eline-C", "name": { "family": "Brombacher", "given": "Eline C." }, "orcid": "0000-0003-4475-5854" }, { "id": "White-William-T", "name": { "family": "White", "given": "William T." }, "orcid": "0000-0001-9705-2453" }, { "id": "Roehl-Henry-H", "name": { "family": "Roehl", "given": "Henry H." }, "orcid": "0000-0001-6497-2405" }, { "id": "Tulenko-Frank-J", "name": { "family": "Tulenko", "given": "Frank J." }, "orcid": "0000-0003-3142-3551" }, { "id": "Winkler-Christoph", "name": { "family": "Winkler", "given": "Christoph" }, "orcid": "0000-0003-4688-6241" }, { "id": "Currie-Peter-D", "name": { "family": "Currie", "given": "Peter D." }, "orcid": "0000-0001-8874-8862" }, { "id": "Amaya-Enrique", "name": { "family": "Amaya", "given": "Enrique" }, "orcid": "0000-0002-1805-8548" }, { "id": "Davis-Marcus-C", "name": { "family": "Davis", "given": "Marcus C." }, "orcid": "0000-0002-2462-0138" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Mosimann-Christian", "name": { "family": "Mosimann", "given": "Christian" }, "orcid": "0000-0002-0749-2576" }, { "id": "Carney-Tom-J", "name": { "family": "Carney", "given": "Tom J." }, "orcid": "0000-0003-2371-1924" } ] }, "title": "A median fin derived from the lateral plate mesoderm and the origin of paired fins", "ispublished": "pub", "full_text_status": "public", "keywords": "Multidisciplinary", "note": "© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
\n\nWe thank K. Poss and M. Tanaka for providing zebrafish and medaka hand2 plasmids, respectively; Z. Gong for the transgenic fish line TgBAC(hand2:EGFP); D. Traver for input on the first iteration of Dendra2 transgenics; Y.-K. Tea for input; and K. P. Lim of the Lee Kong Chian Natural History Museum, Singapore for assisting with the frilled shark specimen. We also thank the Nanyang Technological University Zebrafish facility, the NTU Optical Bio-Imaging Centre (NOBIC), Colorado University (CU) Anschutz zebrafish care staff and all past and present members of our teams for continued support. This work was funded by the Industry Aligned Fund (IAF) Agency for Science, Technology and Research (grant to T.J.C. and K.-W.T.); Ministry of Education (MoE) Tier 3 (grant 2016-T3-1-005 to T.J.C., C.W. and H.M.); Ministry of Education (MoE) Tier 1 (grant 2016-T1-001-055 to T.J.C. and C.Z.); Ministry of Education (MoE) Tier 2 (grant MOE-T2EP30221-0008 to C.W.); the Company of Biologists (travelling fellowship to M.J.T.); the National Science Foundation (grants IOS-1853949 to M.C.D. and 2203311 to C.M.); the Swiss National Science Foundation Sinergia (grant CRSII5_180345 to C.M.); the Swiss Bridge Foundation (C.M.); Additional Ventures Single Ventricle Research Fund (SVRF) (grant 1048003 to C.M.); the University of Colorado School of Medicine Anschutz Medical Campus and the Children’s Hospital Colorado Foundation (C.M.); the National Institutes of Health (NIH), National Institute of General Medical Sciences (grants 1T32GM141742-01 to H.R.M. and 3T32GM121742-02S1 to H.R.M.); Australian Research Council (discovery grant DP200103219 to F.J.T. and P.D.C); National Health and Medical Research Council (senior principal research fellow APP1136567 to P.D.C.); and the NIH (grant R35NS111564 to J.S. and M.E.B.). Jan Stundl is supported by funding from the European Union’s Horizon 2020 research and innovation program under Marie Sklodowska-Curie grant agreement No. 897949.
\nExperiments were conceived and designed by T.J.C., C.M., K.-W.T., M.E.B., M.C.D., E.A., P.D.C., C.W. and H.H.R. and were performed and analysed by K.-W.T., R.L.L., K.D.P., J.S., H.M., H.R.M., A.N.C., Y.L., R.L., K.D., M.J.T., C.Z., E.C.B., W.T.W. and F.J.T. The manuscript was written by K.-W.T. and T.J.C., with editing and input from C.M., J.S., A.N.C., M.C.D., F.J.T., P.D.C., R.L.L., K.D.P. and E.A.
\n\nThe authors confirm that all relevant data are provided in this paper and in its Extended Data files. The data for measurements of fin size and cell number in Extended Data Fig. 3m–o are available in Figshare with the identifier https://doi.org/10.6084/m9.figshare.22269769. Source data are provided with this paper.
\n\nThe authors declare no competing interests.
\nThe development of paired appendages was a key innovation during evolution and facilitated the aquatic to terrestrial transition of vertebrates. Largely derived from the lateral plate mesoderm (LPM), one hypothesis for the evolution of paired fins invokes derivation from unpaired median fins via a pair of lateral fin folds located between pectoral and pelvic fin territories1. Whilst unpaired and paired fins exhibit similar structural and molecular characteristics, no definitive evidence exists for paired lateral fin folds in larvae or adults of any extant or extinct species. As unpaired fin core components are regarded as exclusively derived from paraxial mesoderm, any transition presumes both co-option of a fin developmental programme to the LPM and bilateral duplication2. Here, we identify that the larval zebrafish unpaired pre-anal fin fold (PAFF) is derived from the LPM and thus may represent a developmental intermediate between median and paired fins. We trace the contribution of LPM to the PAFF in both cyclostomes and gnathostomes, supporting the notion that this is an ancient trait of vertebrates. Finally, we observe that the PAFF can be bifurcated by increasing bone morphogenetic protein signalling, generating LPM-derived paired fin folds. Our work provides evidence that lateral fin folds may have existed as embryonic anlage for elaboration to paired fins.
\nAccepted Version - nihms-1897881.pdf
", "abstract": "During development of the vertebrate sensory system, many important components like the sense organs and cranial sensory ganglia arise within the head and neck. Two progenitor populations, the neural crest, and cranial ectodermal placodes, contribute to these developing vertebrate peripheral sensory structures. The interactions and contributions of these cell populations to the development of the lens, olfactory, otic, pituitary gland, and cranial ganglia are vital for appropriate peripheral nervous system development. Here, we review the origins of both neural crest and placode cells at the neural plate border of the early vertebrate embryo and investigate the molecular and environmental signals that influence specification of different sensory regions. Finally, we discuss the underlying molecular pathways contributing to the complex vertebrate sensory system from an evolutionary perspective, from basal vertebrates to amniotes.", "date": "2023-03-30", "date_type": "published", "publication": "Seminars in Cell and Developmental Biology", "volume": "138", "publisher": "Elsevier", "pagerange": "15-27", "id_number": "CaltechAUTHORS:20220705-346538000", "issn": "1084-9521", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220705-346538000", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "5F31 DE027583-03" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "5F31 DE031154-02" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.semcdb.2022.06.009", "pmcid": "PMC10224775", "primary_object": { "basename": "nihms-1897881.pdf", "url": "https://authors.library.caltech.edu/records/v1pgg-aq098/files/nihms-1897881.pdf" }, "resource_type": "article", "pub_year": "2023", "author_list": "Koontz, Alison; Urrutia, Hugo A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w4pze-0zg66", "eprint_id": 120122, "eprint_status": "archive", "datestamp": "2023-08-20 16:43:07", "lastmod": "2023-12-13 16:45:10", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bernardi-Yanel-E", "name": { "family": "Bernardi", "given": "Yanel E." } }, { "id": "Sanchez-Vasquez-Estefania", "name": { "family": "Sanchez-Vasquez", "given": "Estefania" }, "orcid": "0000-0002-6585-8548" }, { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Urrutia-Hugo", "name": { "family": "Urrutia", "given": "Hugo" }, "orcid": "0000-0002-2970-6918" }, { "id": "Rossi-Izadora-V", "name": { "family": "Rossi", "given": "Izadora" }, "orcid": "0000-0001-5495-8167" }, { "id": "Alcantara-Saraiva-Karina-L", "name": { "family": "Alcantara Saraiva", "given": "Karina L." } }, { "id": "Pereira-Neves-Antonio", "name": { "family": "Pereira-Neves", "given": "Antonio" }, "orcid": "0000-0001-6629-568X" }, { "id": "Ramirez-Marcel-Ivan", "name": { "family": "Ramirez", "given": "Marcel Ivan" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "de Miguel-Natalia", "name": { "family": "de Miguel", "given": "Natalia" }, "orcid": "0000-0002-3864-0703" }, { "id": "Strobl-Mazzulla-Pablo-Hern\u00e1n", "name": { "family": "Strobl-Mazzulla", "given": "Pablo Hernan" }, "orcid": "0000-0003-0591-6168" } ] }, "title": "Extracellular vesicle-localized miR-203 mediates neural crest-placode communication required for trigeminal ganglia formation", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. \n\nWe thank all the authors and members in the Laboratory of Developmental Biology at the INTECH for their contribution and helpful discussions during the course of our study. We thank Dr. Alissa M. Weaver (Vanderbilt University School of Medicine, Nashville, TN, USA) for the pHluo construct. We are very grateful to the directors and students from \"Escuela de Educaci\u00f3n Secundaria Agraria de Chascom\u00fas\" for providing fertilized eggs of excellent quality. \n\nThis work was supported by the Agencia Nacional de Promoci\u00f3n Cient\u0131\u0301fica y Tecnol\u00f3gica (PICT 2018-1879 to P.H.S-M.) and by the Fogarty International Center of the National Institutes of Health (R21TW011224 to M.E.B. and P.H.S-M.) \n\nAuthor contributions: Y.E.B. and P.H.S-M. designed, performed the experiments and wrote the manuscript with editing and input from all coauthors; E.S-V performed experiments; M.L.P. and H.U. contributed in the co-culture and time-lapse experiments; I.R and M.I.R contributed on the NTA analysis of sEVs; K.L.A.S and A.P-N. performed EM imaging and aided in their interpretation; M.E.B contributed in the discussion, experimental design and manuscript writing; N.d.M. contributed on the sEVs purifications, characterization, experimental design and manuscript writing. \n\nThe authors have declared no competing interest.\n\nSubmitted - 2023.03.14.532527v1.full.pdf
", "abstract": "While interactions between neural crest and placode cells are critical for the proper formation of the trigeminal ganglion, the mechanisms underlying this process remain largely uncharacterized. Here, we show that the microRNA-(miR)203, whose epigenetic repression is required for neural crest migration, is reactivated in coalescing and condensing trigeminal ganglion cells. Overexpression of miR-203 induces ectopic coalescence of neural crest cells and increases ganglion size. Reciprocally, loss of miR-203 function in placode, but not neural crest, cells perturbs trigeminal ganglion condensation. Demonstrating intercellular communication, overexpression of miR-203 in the neural crest in vitro or in vivo represses a miR-responsive sensor in placode cells. Moreover, neural crest-secreted extracellular vesicles (EVs), visualized using pHluorin-CD63 vector, become incorporated into the cytoplasm of placode cells. Finally, RT-PCR analysis shows that small EVs isolated from condensing trigeminal ganglia are selectively loaded with miR-203. Together, our findings reveal a critical role in vivo for neural crest-placode communication mediated by sEVs and their selective microRNA cargo for proper trigeminal ganglion formation.", "date": "2023-03-22", "date_type": "published", "id_number": "CaltechAUTHORS:20230316-181901000.3", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230316-181901000.3", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Agencia Nacional de Promoci\u00f3n Cient\u0131\u0301fica y Tecnol\u00f3gica", "grant_number": "PICT 2018-1879" }, { "agency": "NIH", "grant_number": "R21TW011224" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2023.03.14.532527", "primary_object": { "basename": "2023.03.14.532527v1.full.pdf", "url": "https://authors.library.caltech.edu/records/w4pze-0zg66/files/2023.03.14.532527v1.full.pdf" }, "resource_type": "monograph", "pub_year": "2023", "author_list": "Bernardi, Yanel E.; Sanchez-Vasquez, Estefania; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g43wp-8n433", "eprint_id": 119589, "eprint_status": "archive", "datestamp": "2023-08-22 19:08:10", "lastmod": "2023-12-22 23:23:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mehrotra-Pihu", "name": { "family": "Mehrotra", "given": "Pihu" }, "orcid": "0000-0001-9369-5013" }, { "id": "Ikhapoh-Izuagie", "name": { "family": "Ikhapoh", "given": "Izuagie" } }, { "id": "Lei-Pedro", "name": { "family": "Lei", "given": "Pedro" } }, { "id": "Tseropoulos-Georgios", "name": { "family": "Tseropoulos", "given": "Georgios" } }, { "id": "Zhang-Yali", "name": { "family": "Zhang", "given": "Yali" } }, { "id": "Wang-Jianmin", "name": { "family": "Wang", "given": "Jianmin" }, "orcid": "0000-0001-7527-0409" }, { "id": "Liu-Song", "name": { "family": "Liu", "given": "Song" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Andreadis-Stelios-T", "name": { "family": "Andreadis", "given": "Stelios T." } } ] }, "title": "Wnt/BMP Mediated Metabolic Reprogramming Preserves Multipotency of Neural Crest-Like Stem Cells", "ispublished": "pub", "full_text_status": "public", "keywords": "Cell Biology; Developmental Biology; Molecular Medicine", "note": "\u00a9 The Author(s) 2023. Published by Oxford University Press. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights) \n\nThis work was supported by grants from the National Institutes of Health R01 EB023114 (S.T.A.) and the New York Stem Cell Science NYSTEM (Contract #C30290GG, S.T.A.).", "abstract": "Neural crest-like stem cells resembling embryonic neural crest cells (NCs) can be derived from adult human tissues such as the epidermis. However, these cells lose their multipotency rapidly in culture limiting their expansion for clinical use. Here, we show that the multipotency of keratinocyte-derived NCs (KC-NCs) can be preserved by activating the Wnt and BMP signaling axis, promoting expression of key NC-specifier genes and ultimately enhancing their differentiation potential. We also show that transcriptional changes leading to multipotency are linked to metabolic reprogramming of KC-NCs to a highly glycolytic state. Specifically, KC-NCs treated with CHIR and BMP2 rely almost exclusively on glycolysis for their energy needs, as seen by increased lactate production, glucose uptake, and glycolytic enzyme activities. This was accompanied by mitochondrial depolarization and decreased mitochondrial ATP production. Interestingly, the glycolytic end-product lactate stabilized \u03b2-catenin and further augmented NC-gene expression. Taken together, our study shows that activation of the Wnt/BMP signaling coordinates the metabolic demands of neural crest-like stem cells governing decisions regarding multipotency and differentiation, with possible implications for regenerative medicine.", "date": "2023-03", "date_type": "published", "publication": "Stem Cells", "volume": "41", "number": "3", "publisher": "Wiley", "pagerange": "287-305", "id_number": "CaltechAUTHORS:20230228-419577900.6", "issn": "1066-5099", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230228-419577900.6", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "EB023114" }, { "agency": "New York Stem Cell Foundation", "grant_number": "C30290GG" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1093/stmcls/sxad001", "pmcid": "PMC10020983", "resource_type": "article", "pub_year": "2023", "author_list": "Mehrotra, Pihu; Ikhapoh, Izuagie; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3yav7-2rc44", "eprint_id": 122534, "eprint_status": "archive", "datestamp": "2023-08-22 18:29:42", "lastmod": "2024-01-18 23:20:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lin-Lizhu", "name": { "family": "Lin", "given": "Lizhu" } }, { "id": "Pinto-Antonella", "name": { "family": "Pinto", "given": "Antonella" } }, { "id": "Wang-Lu", "name": { "family": "Wang", "given": "Lu" }, "orcid": "0000-0001-5263-3123" }, { "id": "Fukatsu-Kazumi", "name": { "family": "Fukatsu", "given": "Kazumi" } }, { "id": "Yin-Yan", "name": { "family": "Yin", "given": "Yan" }, "orcid": "0000-0002-8391-2712" }, { "id": "Bamforth-Simon-D", "name": { "family": "Bamforth", "given": "Simon D." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Evans-Sylvia-M", "name": { "family": "Evans", "given": "Sylvia M." } }, { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" }, "orcid": "0000-0002-0495-7104" }, { "id": "Anderson-Robert-H", "name": { "family": "Anderson", "given": "Robert H." } }, { "id": "Terskikh-Alexey-V", "name": { "family": "Terskikh", "given": "Alexey V." } }, { "id": "Grossfeld-Paul-D", "name": { "family": "Grossfeld", "given": "Paul D." }, "orcid": "0000-0001-7215-3821" } ] }, "title": "ETS1 loss in mice impairs cardiac outflow tract septation via a cell migration defect autonomous to the neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Genetics (clinical); Genetics; Molecular Biology; General Medicine", "note": "\u00a9 The Author(s) 2022. Published by Oxford University Press. \n\nThe authors would like to thank Dr Michael Ostrowski for providing the Ets1 floxed mice. The authors also would like to thank the UCSD School of Medicine Microscopy Core (Grant: NINDS P30 NS047101) for their invaluable technical support. \n\nFunding: American Heart Association (#16GRNT26700008); The Hertz Family Foundation; The Rady Children's Hospital Foundation; The 11q Research and Resource Group; The Chloe Duyck Memorial Fund; The Warren J. and Betty C. Zable Foundation and the cast and crew of 'How I Met Your Mother'. \n\nThe authors have no disclosures.\n\nPublished - ddac174.pdf
", "abstract": "Ets1 deletion in some mouse strains causes septal defects and has been implicated in human congenital heart defects in Jacobsen syndrome, in which one copy of the Ets1 gene is missing. Here, we demonstrate that loss of Ets1 in mice results in a decrease in neural crest (NC) cells migrating into the proximal outflow tract cushions during early heart development, with subsequent malalignment of the cushions relative to the muscular ventricular septum, resembling double outlet right ventricle (DORV) defects in humans. Consistent with this, we find that cultured cardiac NC cells from Ets1 mutant mice or derived from iPS cells from Jacobsen patients exhibit decreased migration speed and impaired cell-to-cell interactions. Together, our studies demonstrate a critical role for ETS1 for cell migration in cardiac NC cells that are required for proper formation of the proximal outflow tracts. These data provide further insights into the molecular and cellular basis for development of the outflow tracts, and how perturbation of NC cells can lead to DORV.", "date": "2022-12-15", "date_type": "published", "publication": "Human Molecular Genetics", "volume": "31", "number": "24", "publisher": "Oxford University Press", "pagerange": "4217-4227", "id_number": "CaltechAUTHORS:20230725-706619000.50", "issn": "0964-6906", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230725-706619000.50", "funders": { "items": [ { "agency": "NIH", "grant_number": "P30 NS047101" }, { "agency": "American Heart Association", "grant_number": "16GRNT26700008" }, { "agency": "Hertz Family Foundation" }, { "agency": "Rady Children's Hospital Foundation" }, { "agency": "11q Research and Resource Group" }, { "agency": "Chloe Duyck Memorial Fund" }, { "agency": "Warren J. and Betty C. Zable Foundation" }, { "agency": "How I Met Your Mother" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1093/hmg/ddac174", "pmcid": "PMC10148727", "primary_object": { "basename": "ddac174.pdf", "url": "https://authors.library.caltech.edu/records/3yav7-2rc44/files/ddac174.pdf" }, "resource_type": "article", "pub_year": "2022", "author_list": "Lin, Lizhu; Pinto, Antonella; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/yfatn-c4a08", "eprint_id": 122428, "eprint_status": "archive", "datestamp": "2023-08-22 18:28:10", "lastmod": "2023-12-22 23:40:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Andrews-Cecelia-J", "name": { "family": "Andrews", "given": "Cecelia J." }, "orcid": "0000-0003-2734-0542" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition", "ispublished": "pub", "full_text_status": "public", "keywords": "Multidisciplinary", "note": "\u00a9 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). \n\nFunding for this work comes from the NIH grants K99DE029240 to M.L.P., K99DE028592 to E.J.H., R01DE027538 and R01DE027568 to M.E.B., and from the Caltech Summer Undergraduate Research Fellowship (SURF) to C.J.A. \n\nAuthor Contributions. M.L.P. and M.E.B. designed research; M.L.P., E.J.H., and C.J.A. performed research; M.L.P. contributed new reagents/analytic tools; M.L.P., E.J.H., and C.J.A. analyzed data; M.L.P. wrote the manuscript; and E.J.H. and M.E.B. edited the manuscript. \n\nData, Materials, and Software Availability. All study data are included in the main text and/or supporting information. Source data can also be found with data analysis codes on GitHub at https://github.com/PiacentinoLab/2022_PNAS_nSMase2_Endocytosis. The Smpd3 mRNA sequence has been submitted to GenBank (Accession #MW142015). \n\nThe authors declare no competing interest.\n\nPublished - pnas.202212879.pdf
Supplemental Material - pnas.2212879119.sapp.pdf
", "abstract": "Epithelial-to-mesenchymal transition (EMT) is a dramatic change in cellular physiology during development and metastasis, which requires coordination between cell signaling, adhesion, and membrane protrusions. These processes all involve dynamic changes in the plasma membrane; yet, how membrane lipid content regulates membrane function during EMT remains incompletely understood. By screening for differential expression of lipid-modifying genes over the course of EMT in the avian neural crest, we have identified the ceramide-producing enzyme neutral sphingomyelinase 2 (nSMase2) as a critical regulator of a developmental EMT. nSMase2 expression begins at the onset of EMT, and in vivo knockdown experiments demonstrate that nSMase2 is necessary for neural crest migration. We find that nSMase2 promotes Wnt and BMP signaling and is required to activate the mesenchymal gene expression program. Mechanistically, we show that nSMase2-dependent ceramide production is necessary for and sufficient to up-regulate endocytosis and is required for Wnt co-receptor internalization. Finally, inhibition of endocytosis in the neural crest mimics the loss of migration and Wnt signaling observed following nSMase2 knockdown. Our results support a model in which nSMase2 is expressed at the onset of neural crest EMT to produce ceramide and facilitate receptor-mediated endocytosis of Wnt and BMP signaling complexes, thereby activating promigratory gene expression. These results highlight the critical role of plasma membrane lipid metabolism in regulating transcriptional changes during developmental EMT programs.", "date": "2022-12-12", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "119", "number": "51", "publisher": "National Academy of Sciences", "pagerange": "Art. No. e2212879119", "id_number": "CaltechAUTHORS:20230725-49081000.12", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230725-49081000.12", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Caltech Beckman Institute" }, { "agency": "Arnold and Mabel Beckman Foundation" }, { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" } ] }, "local_group": { "items": [ { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1073/pnas.2212879119", "pmcid": "PMC9907157", "primary_object": { "basename": "pnas.202212879.pdf", "url": "https://authors.library.caltech.edu/records/yfatn-c4a08/files/pnas.202212879.pdf" }, "related_objects": [ { "basename": "pnas.2212879119.sapp.pdf", "url": "https://authors.library.caltech.edu/records/yfatn-c4a08/files/pnas.2212879119.sapp.pdf" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Piacentino, Michael L.; Hutchins, Erica J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nft7r-esq66", "eprint_id": 120357, "eprint_status": "archive", "datestamp": "2023-08-22 17:54:11", "lastmod": "2023-12-22 23:25:51", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Chacon-Jose", "name": { "family": "Chacon", "given": "Jose" }, "orcid": "0000-0001-7965-3976" }, { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael" }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification", "ispublished": "pub", "full_text_status": "public", "keywords": "General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Medicine; General Neuroscience", "note": "\u00a9 2022, Hutchins et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. \n\nWe thank A Collazo and G Spigolon for imaging assistance at the Caltech Biological Imaging Facility; M Schwarzkopf and G Shin (Molecular Technologies) for HCR probe design; I Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for sequencing of RNA-seq libraries; S Manohar for assistance with Axud1 3\u2032-RACE; and G da Silva Pescador and R Galton for assistance with pilot experiments. \n\nData availability. RNA-sequencing datasets have been deposited on NCBI under the accession number PRJNA861325. The 3' untranslated region (UTR) sequence for Axud1 has been deposited to GenBank under accession number ON920861.\n\nPublished - elife-63600.pdf
", "abstract": "While neural crest development is known to be transcriptionally controlled via sequential activation of gene regulatory networks (GRNs), recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using available single-cell RNA-sequencing datasets from avian embryos to identify potential post-transcriptional regulators, we found that Elavl1, which encodes for an RNA-binding protein with roles in transcript stability, was enriched in the premigratory cranial neural crest. Perturbation of Elavl1 resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN, phenotypes that are also observed with downregulation of the canonical Wnt inhibitor Draxin. That Draxin is the primary target for stabilization by Elavl1 during cranial neural crest specification was shown by RNA-sequencing, RNA immunoprecipitation, RNA decay measurement, and proximity ligation assays, further supporting the idea that the downregulation of neural crest specifier expression upon Elavl1 knockdown was largely due to loss of Draxin. Importantly, exogenous Draxin rescued cranial neural crest specification defects observed with Elavl1 knockdown. Thus, Elavl1 plays a critical a role in the maintenance of cranial neural crest specification via Draxin mRNA stabilization. Together, these data highlight an important intersection of post-transcriptional regulation with modulation of the neural crest specification GRN.", "date": "2022-10-03", "date_type": "published", "publication": "eLife", "volume": "11", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e63600", "id_number": "CaltechAUTHORS:20230322-368501000.44", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230322-368501000.44", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "Amgen Foundation" }, { "agency": "California State University, Northridge", "grant_number": "TL4GM118977" }, { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "R01DE027568" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.7554/elife.63600", "pmcid": "PMC9529247", "primary_object": { "basename": "elife-63600.pdf", "url": "https://authors.library.caltech.edu/records/nft7r-esq66/files/elife-63600.pdf" }, "resource_type": "article", "pub_year": "2022", "author_list": "Hutchins, Erica J.; Gandhi, Shashank; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/p6rwh-7ns85", "eprint_id": 121320, "eprint_status": "archive", "datestamp": "2023-08-22 17:37:34", "lastmod": "2023-12-22 23:25:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Zhang-Haifeng", "name": { "family": "Zhang", "given": "Haifeng" }, "orcid": "0000-0002-5126-4490" }, { "id": "Shang-Renjie", "name": { "family": "Shang", "given": "Renjie" }, "orcid": "0000-0002-9184-4094" }, { "id": "Kim-Kwantae", "name": { "family": "Kim", "given": "Kwantae" } }, { "id": "Zheng-Wei", "name": { "family": "Zheng", "given": "Wei" }, "orcid": "0000-0002-2984-9003" }, { "id": "Johnson-Christopher-J", "name": { "family": "Johnson", "given": "Christopher J." } }, { "id": "Sun-Lei", "name": { "family": "Sun", "given": "Lei" }, "orcid": "0000-0001-8741-0202" }, { "id": "Niu-Xiang", "name": { "family": "Niu", "given": "Xiang" }, "orcid": "0000-0001-7689-0941" }, { "id": "Liu-Liang", "name": { "family": "Liu", "given": "Liang" }, "orcid": "0000-0003-0568-4264" }, { "id": "Zhou-Jingqi", "name": { "family": "Zhou", "given": "Jingqi" }, "orcid": "0000-0002-9961-199X" }, { "id": "Liu-Lingshu", "name": { "family": "Liu", "given": "Lingshu" } }, { "id": "Zhang-Zheng", "name": { "family": "Zhang", "given": "Zheng" } }, { "id": "Uyeno-Theodore-A", "name": { "family": "Uyeno", "given": "Theodore A." }, "orcid": "0000-0001-8998-5595" }, { "id": "Pei-Jimin", "name": { "family": "Pei", "given": "Jimin" }, "orcid": "0000-0002-3505-9665" }, { "id": "Fissette-Skye-D", "name": { "family": "Fissette", "given": "Skye D." }, "orcid": "0000-0003-0595-2919" }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." }, "orcid": "0000-0002-2831-3610" }, { "id": "Samudra-Sukhada-P", "name": { "family": "Samudra", "given": "Sukhada P." }, "orcid": "0000-0002-7006-8970" }, { "id": "Wen-Junfei", "name": { "family": "Wen", "given": "Junfei" }, "orcid": "0000-0001-8267-6124" }, { "id": "Zhang-Jianli", "name": { "family": "Zhang", "given": "Jianli" } }, { "id": "Eggenschwiler-Jonathan-T", "name": { "family": "Eggenschwiler", "given": "Jonathan T." }, "orcid": "0000-0001-6444-8865" }, { "id": "Menke-Douglas-B", "name": { "family": "Menke", "given": "Douglas B." }, "orcid": "0000-0002-7109-1451" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Grishin-Nick-V", "name": { "family": "Grishin", "given": "Nick V." }, "orcid": "0000-0003-4108-1153" }, { "id": "Li-Weiming", "name": { "family": "Li", "given": "Weiming" }, "orcid": "0000-0001-5437-1518" }, { "id": "Ye-Kaixiong", "name": { "family": "Ye", "given": "Kaixiong" }, "orcid": "0000-0003-4658-7292" }, { "id": "Zhang-Yang", "name": { "family": "Zhang", "given": "Yang" }, "orcid": "0000-0002-2739-1916" }, { "id": "Stolfi-Alberto", "name": { "family": "Stolfi", "given": "Alberto" }, "orcid": "0000-0001-7179-9700" }, { "id": "Bi-Pengpeng", "name": { "family": "Bi", "given": "Pengpeng" }, "orcid": "0000-0002-9871-6773" } ] }, "title": "Evolution of a chordate-specific mechanism for myoblast fusion", "ispublished": "pub", "full_text_status": "public", "keywords": "Multidisciplinary", "note": "\u00a9 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). \n\nWe thank trainees G. Gopu, A. Baiju, and E. M. Hicks in Bi laboratory and A. L. Womble from Valdosta State University for technical help. We are grateful to E. N. Olson from University of Texas Southwestern Medical Center for critical reading of the manuscript. We thank the following collaborators for advice: H. Li from Ocean University of China; C. Ca\u00f1estro from University of Barcelona; S. Kuraku, R. Kusakabe, and S. Kuratani from RIKEN; S. Du from University of Maryland School of Medicine; J. Ziermann from Howard University; Z. Yang from University of College London; F. Razy-Krajka and S. Tiozzo from Sorbonne/CNRS/Villefranche-sur-Mer; B. Davidson and C. J. Pickett from Swarthmore College; J.F. Ryan from University of Florida; and M. Frischer from University of Georgia. A. Bigot and V. Mouly from the Myoline platform of the Myology Institute provided myoblast cell lines. X. Li from University of Texas Southwestern Medical Center, N. S. Johnson from U.S. Geological Survey, M. Brindley from University of Georgia provided materials and reagents. \n\nThis work was supported by the starting up fund from the University of Georgia to P.B., NIH R01 award GM143326 and NSF award 1940743 to A.S., an NSF Graduate Research Fellowship to C.J.J., Great Lakes Fishery Commission (540810) to S.D.F. and W.L., NSF award 1354788 to T.A.U., and NSF award 1827647 to D.B.M. and J.T.E. \n\nAuthor contributions: H.Z., A.S., and P.B. designed research; H.Z., R.S., K.K., W.Z., C.J.J., L.S., X.N., Liang Liu, J. Zhou, Lingshu Liu, Z.Z., T.A.U., J.P., S.D.F., S.A.G., S.P.S., J.W., J. Zhang, J.T.E., D.B.M., M.E.B., N.V.G., W.L., K.Y., Y.Z., A.S., and P.B. performed research; H.Z., R.S., K.K., W.Z., C.J.J., L.S., X.N., Liang Liu, J. Zhang, Lingshu Liu, J.W., W.L., K.Y., Y.Z., A.S., and P.B. analyzed data. A.S. and P.B. wrote the paper. \n\nData and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. The immortalized lizard embryonic cell line (ASEC-1) will be provided by D.B.M. pending scientific review and a completed material transfer agreement. Requests for the ASEC-1 cell line should be submitted to D.B.M.\n\nPublished - sciadv.add2696.pdf
Supplemental Material - sciadv.add2696_movies_s1_to_s4.zip
Supplemental Material - sciadv.add2696_sm.pdf
Supplemental Material - sciadv.add2696_supplemental_files_1_to_3.zip
Supplemental Material - sciadv.add2696_tables_s1_and_s2.zip
", "abstract": "Vertebrate myoblast fusion allows for multinucleated muscle fibers to compound the size and strength of mononucleated cells, but the evolution of this important process is unknown. We investigated the evolutionary origins and function of membrane-coalescing agents Myomaker and Myomixer in various groups of chordates. Here, we report that\n Myomaker\n likely arose through gene duplication in the last common ancestor of tunicates and vertebrates, while\n Myomixer\n appears to have evolved de novo in early vertebrates. Functional tests revealed a complex evolutionary history of myoblast fusion. A prevertebrate phase of muscle multinucleation driven by Myomaker was followed by the later emergence of Myomixer that enables the highly efficient fusion system of vertebrates. Evolutionary comparisons between vertebrate and nonvertebrate Myomaker revealed key structural and mechanistic insights into myoblast fusion. Thus, our findings suggest an evolutionary model of chordate fusogens and illustrate how new genes shape the emergence of novel morphogenetic traits and mechanisms.", "date": "2022-09-02", "date_type": "published", "publication": "Science Advances", "volume": "8", "number": "35", "publisher": "American Association for the Advancement of Science", "pagerange": "Art. No. eadd2696", "id_number": "CaltechAUTHORS:20230504-446162300.3", "issn": "2375-2548", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20230504-446162300.3", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1126/sciadv.add2696", "primary_object": { "basename": "sciadv.add2696.pdf", "url": "https://authors.library.caltech.edu/records/p6rwh-7ns85/files/sciadv.add2696.pdf" }, "related_objects": [ { "basename": "sciadv.add2696_movies_s1_to_s4.zip", "url": "https://authors.library.caltech.edu/records/p6rwh-7ns85/files/sciadv.add2696_movies_s1_to_s4.zip" }, { "basename": "sciadv.add2696_sm.pdf", "url": "https://authors.library.caltech.edu/records/p6rwh-7ns85/files/sciadv.add2696_sm.pdf" }, { "basename": "sciadv.add2696_supplemental_files_1_to_3.zip", "url": "https://authors.library.caltech.edu/records/p6rwh-7ns85/files/sciadv.add2696_supplemental_files_1_to_3.zip" }, { "basename": "sciadv.add2696_tables_s1_and_s2.zip", "url": "https://authors.library.caltech.edu/records/p6rwh-7ns85/files/sciadv.add2696_tables_s1_and_s2.zip" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Zhang, Haifeng; Shang, Renjie; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t3dqm-ksv15", "eprint_id": 115460, "eprint_status": "archive", "datestamp": "2023-08-22 17:36:03", "lastmod": "2023-12-22 23:13:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kastriti-Maria-Eleni", "name": { "family": "Kastriti", "given": "Maria Eleni" }, "orcid": "0000-0002-0563-7399" }, { "id": "Faure-Louis", "name": { "family": "Faure", "given": "Louis" }, "orcid": "0000-0003-4621-586X" }, { "id": "Von-Ahsen-Dorothea", "name": { "family": "Von Ahsen", "given": "Dorothea" } }, { "id": "Bouderlique-Thibault-Gerald", "name": { "family": "Bouderlique", "given": "Thibault Gerald" } }, { "id": "Bostr\u00f6m-Johan", "name": { "family": "Bostr\u00f6m", "given": "Johan" }, "orcid": "0000-0001-5252-4023" }, { "id": "Solovieva-Tatiana", "name": { "family": "Solovieva", "given": "Tatiana" }, "orcid": "0000-0001-6194-2550" }, { "id": "Jackson-Cameron", "name": { "family": "Jackson", "given": "Cameron" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Meijer-Dies", "name": { "family": "Meijer", "given": "Dies" }, "orcid": "0000-0002-8461-6341" }, { "id": "Hadjab-Saida", "name": { "family": "Hadjab", "given": "Saida" } }, { "id": "Lallemend-Francois", "name": { "family": "Lallemend", "given": "Francois" } }, { "id": "Erickson-Alek", "name": { "family": "Erickson", "given": "Alek" } }, { "id": "Kaucka-Marketa", "name": { "family": "Kaucka", "given": "Marketa" }, "orcid": "0000-0002-8781-9769" }, { "id": "Oyacxhuk-Viacheslav", "name": { "family": "Dyachuk", "given": "Viacheslav" } }, { "id": "Perlmann-Thomas", "name": { "family": "Perlmann", "given": "Thomas" } }, { "id": "Lahti-Laura", "name": { "family": "Lahti", "given": "Laura" } }, { "id": "Krivanek-Jan", "name": { "family": "Krivanek", "given": "Jan" }, "orcid": "0000-0002-7590-187X" }, { "id": "Brunet-Jean-Fran\u00e7ois", "name": { "family": "Brunet", "given": "Jean-Fran\u00e7ois" } }, { "id": "Fried-Kaj", "name": { "family": "Fried", "given": "Kaj" } }, { "id": "Adameyko-Igor", "name": { "family": "Adameyko", "given": "Igor" }, "orcid": "0000-0001-5471-0356" } ] }, "title": "Schwann cell precursors represent a neural crest\u2010like state with biased multipotency", "ispublished": "pub", "full_text_status": "public", "keywords": "multipotency; neural crest; regulons; Schwann cell precursors; Schwann cell lineage; General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; Molecular Biology; General Neuroscience", "note": "\u00a92022 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. \n\nReceived: 20 May 2021. Accepted: 15 June 2022. Published: 11 July 2022. \n\nThe authors want to thank the Eukaryotic Single-Cell Genomics facility (Stockholm, Sweden) and in particular Henrik Gezelius and Anastasios Glaros for excellent sequencing services and customer support. MEK was supported by the Novo Nordisk Foundation (Postdoc fellowship in Endocrinology and Metabolism at International Elite Environments, NNF17OC0026874) and Stiftelsen Riksbankens Jubileumsfond (Erik R\u00f6nnbergs fond stipend). LF was supported by Austrian Science Fund DOC 33-B27. T.G.B. was supported by a Lise Meitner grant from the Austrian Science Fund (M2688-B28). TS was supported by NIH grant DE027568 to MB. SH is supported by Swedish Research Council, Brain Foundation and StratNeuro. VD was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2022-301). TP was supported by the Swedish Research Council (2020-00884); Knut and Alice Wallenberg's Foundation and S\u00f6derberg's Foundation. IA was supported by Paradifference Foundation, The Swedish Cancer Society, Bertil H\u00e5llsten Research Foundation, Swedish Research Council, ERC Consolidator and EMBO Young Investigator Grants. \n\nAuthor contributions:\nMaria Eleni Kastriti: Conceptualization; data curation; formal analysis; supervision; validation; investigation; visualization; writing \u2013 original draft; project administration; writing \u2013 review and editing. Louis Faure: Resources; data curation; software; formal analysis; investigation; visualization; methodology; writing \u2013 original draft; writing \u2013 review and editing. Dorothea Von Ahsen: Formal analysis; validation; investigation; visualization; methodology. Johan Bostr\u00f6m: Resources; investigation; writing \u2013 review and editing. Thibault Gerald Bouderlique: Validation; investigation; visualization; writing \u2013 review and editing. Tatiana Solovieva: Data curation; formal analysis; investigation; visualization; methodology; writing \u2013 review and editing. Cameron Jackson: Investigation. Marianne Bronner: Resources; supervision; funding acquisition; methodology; writing \u2013 review and editing. Dies Meijer: Writing \u2013 review and editing. Saida Hadjab: Resources; funding acquisition; writing \u2013 review and editing. Francois Lallemend: Resources; funding acquisition; writing \u2013 review and editing. Alek Erickson: Resources; writing \u2013 original draft. Marketa Kaucka: Resources; writing \u2013 review and editing. Viacheslav Dyachuk: Resources; writing \u2013 review and editing. Thomas Perlmann: Resources; writing \u2013 review and editing. Laura Lahti: Resources; writing \u2013 review and editing. Jan Krivanek: Resources; investigation; writing \u2013 review and editing. Jean-Francois Brunet: Resources; writing \u2013 review and editing. Kaj Fried: Writing \u2013 review and editing. Igor Adameyko: Conceptualization; supervision; funding acquisition; writing \u2013 original draft; project administration; writing \u2013 review and editing. \n\nData availability:\nNewly generated single-cell transcriptomic data and previously published data sets have been uploaded under GSE201257 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE201257). Two pagoda2 web applications are available on the following link: https://adameykolab.srv.meduniwien.ac.at/glia_gene_umap/ with the (gene-based embedding, and https://adameykolab.srv.meduniwien.ac.at/glia_scenic_umap/ with (SCENIC-based UMAP embedding). \n\nCropped images used for quantification of the \"hub\" markers Itga4, Serpine2 and Sox8 following RNAscope\u00ae in situ hybridization can be found at DOI 10.6084/m9.figshare.19620102. \n\nCode and data for downstream analysis are found on the following repository: https://github.com/LouisFaure/glialfates_paper. \n\nThe authors declare that they have no conflict of interest.\n\nSupplemental Material - embj2021108780-sup-0001-appendix.pdf
Supplemental Material - embj2021108780-sup-0002-evfigs.pdf
Supplemental Material - embj2021108780-sup-0003-datasetev1.pdf
", "abstract": "Schwann cell precursors (SCPs) are nerve-associated progenitors that can generate myelinating and non-myelinating Schwann cells but also are multipotent like the neural crest cells from which they originate. SCPs are omnipresent along outgrowing peripheral nerves throughout the body of vertebrate embryos. By using single-cell transcriptomics to generate a gene expression atlas of the entire neural crest lineage, we show that early SCPs and late migratory crest cells have similar transcriptional profiles characterised by a multipotent \"hub\" state containing cells biased towards traditional neural crest fates. SCPs keep diverging from the neural crest after being primed towards terminal Schwann cells and other fates, with different subtypes residing in distinct anatomical locations. Functional experiments using CRISPR-Cas9 loss-of-function further show that knockout of the common \"hub\" gene Sox8 causes defects in neural crest-derived cells along peripheral nerves by facilitating differentiation of SCPs towards sympathoadrenal fates. Finally, specific tumour populations found in melanoma, neurofibroma and neuroblastoma map to different stages of SCP/Schwann cell development. Overall, SCPs resemble migrating neural crest cells that maintain multipotency and become transcriptionally primed towards distinct lineages.", "date": "2022-09-01", "date_type": "published", "publication": "EMBO Journal", "volume": "41", "number": "17", "publisher": "European Molecular Biology Organization", "pagerange": "Art. no. e108780", "id_number": "CaltechAUTHORS:20220711-652600000", "issn": "0261-4189", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220711-652600000", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Novo Nordisk Foundation", "grant_number": "NNF17OC0026874" }, { "agency": "FWF Der Wissenschaftsfonds", "grant_number": "DOC 33-B27" }, { "agency": "FWF Der Wissenschaftsfonds", "grant_number": "M2688-B28" }, { "agency": "NIH", "grant_number": "DE027568" }, { "agency": "Brain Foundation" }, { "agency": "StratNeuro" }, { "agency": "Ministry of Science and Higher Education of the Russian Federation", "grant_number": "075-15-2022-301" }, { "agency": "Knut and Alice Wallenberg Foundation" }, { "agency": "Bank of Sweden Tercentenary Foundation" }, { "agency": "Swedish Research Council", "grant_number": "2020-00884" }, { "agency": "S\u00f6derberg's Foundation" }, { "agency": "Paradifference Foundation" }, { "agency": "Swedish Cancer Society" }, { "agency": "Bertil H\u00e4llsten Research Foundation" }, { "agency": "European Research Council (ERC)" }, { "agency": "European Molecular Biology Organization (EMBO)" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.15252/embj.2021108780", "pmcid": "PMC9434083", "primary_object": { "basename": "The_EMBO_Journal_-_2022_-_Kastriti_-_Schwann_cell_precursors_represent_a_neural_crest\u2010like_state_with_biased_multipotency.pdf", "url": "https://authors.library.caltech.edu/records/t3dqm-ksv15/files/The_EMBO_Journal_-_2022_-_Kastriti_-_Schwann_cell_precursors_represent_a_neural_crest\u2010like_state_with_biased_multipotency.pdf" }, "related_objects": [ { "basename": "embj2021108780-sup-0001-appendix.pdf", "url": "https://authors.library.caltech.edu/records/t3dqm-ksv15/files/embj2021108780-sup-0001-appendix.pdf" }, { "basename": "embj2021108780-sup-0002-evfigs.pdf", "url": "https://authors.library.caltech.edu/records/t3dqm-ksv15/files/embj2021108780-sup-0002-evfigs.pdf" }, { "basename": "embj2021108780-sup-0003-datasetev1.pdf", "url": "https://authors.library.caltech.edu/records/t3dqm-ksv15/files/embj2021108780-sup-0003-datasetev1.pdf" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Kastriti, Maria Eleni; Faure, Louis; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hq8w3-40f14", "eprint_id": 116235, "eprint_status": "archive", "datestamp": "2023-08-22 17:19:38", "lastmod": "2023-12-22 23:34:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Galton-Riley", "name": { "family": "Galton", "given": "Riley" }, "orcid": "0000-0001-6777-2177" }, { "id": "Fejes-T\u00f3th-K", "name": { "family": "Fejes-T\u00f3th", "given": "Katalin" }, "orcid": "0000-0001-6558-2636" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Co-option of the piRNA pathway to regulate neural crest specification", "ispublished": "pub", "full_text_status": "public", "keywords": "Multidisciplinary", "note": "\u00a9 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). \n\nReceived: 5 November 2021. Accepted: 24 June 2022. \n\nWe thank members of the M.E.B., K.F.-T., and Aravin laboratories for helpful discussions. We thank M. Ninova for advice on RNA-seq analysis and for making the ping-pong script available for our use. We also thank M. Piacentino for advice with imaging analysis and providing Fiji macros for our use. We thank Q. Tang for providing us with the mini CMV promoter. We acknowledge the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for library prep and sequencing of our CRISPR RNA-seq experiment and, in particular, thank I. Antoshechkin for advice on data analysis and ensuring that our small RNA libraries were sequenced during the COVID-19 lockdown. \n\nThis work is supported by NIH grants R01GM110217 to K.F.-T. and R35NS111564 to M.E.B. R.G. was supported by the NSF's GRFP fellowship. \n\nAuthor contributions: Conceptualization: R.G., K.F.-T., and M.E.B. Investigation: R.G. Formal analysis: R.G. Visualization: R.G. Funding acquisition: R.G., K.F.-T., and M.E.B. Supervision: K.F.-T. and M.E.B. Writing (original draft): R.G. Writing (review and editing): R.G., K.F.-T., and M.E.B. \n\nData and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. All raw sequencing data generated for this publication are available through Gene Expression Omnibus. Neural fold RNA-seq data (Fig. 3 and figs. S2 and S4) are available with accession number GSE171615 and small RNA-seq data (Figs. 1 and 3and fig. S3) with GSE171616. Previously published specified neural crest datasets (fig. S2) are available from NCBI BioProject no. PRJNA497902. All scripts used in this publication are included in table S2. \n\nThe authors declare that they have no competing interests.\n\nPublished - sciadv.abn1441.pdf
Supplemental Material - sciadv.abn1441_sm.pdf
", "abstract": "Across Metazoa, Piwi proteins play a critical role in protecting the germline genome through piRNA-mediated repression of transposable elements. In vertebrates, activity of Piwi proteins and the piRNA pathway was thought to be gonad specific. Our results reveal the expression of Piwil1 in a vertebrate somatic cell type, the neural crest. Piwil1 is expressed at low levels throughout the chicken neural tube, peaking in neural crest cells just before the specification event that enables epithelial-to-mesenchymal transition (EMT) and migration into the periphery. Loss of Piwil1 impedes neural crest specification and emigration. Small RNA sequencing reveals somatic piRNAs with sequence signatures of an active ping-pong loop. RNA-seq and functional experiments identify the transposon-derived gene ERNI as Piwil1's target in the neural crest. ERNI, in turn, suppresses Sox2 to precisely control the timing of neural crest specification and EMT. Our data provide mechanistic insight into a novel function of the piRNA pathway as a regulator of somatic development in a vertebrate species.", "date": "2022-08-10", "date_type": "published", "publication": "Science Advances", "volume": "8", "number": "32", "publisher": "American Association for the Advancement of Science", "pagerange": "Art. No. abn1441", "id_number": "CaltechAUTHORS:20220811-456719000", "issn": "2375-2548", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220811-456719000", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01GM110217" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NSF Graduate Research Fellowship" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1126/sciadv.abn1441", "primary_object": { "basename": "sciadv.abn1441.pdf", "url": "https://authors.library.caltech.edu/records/hq8w3-40f14/files/sciadv.abn1441.pdf" }, "related_objects": [ { "basename": "sciadv.abn1441_sm.pdf", "url": "https://authors.library.caltech.edu/records/hq8w3-40f14/files/sciadv.abn1441_sm.pdf" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Galton, Riley; Fejes-T\u00f3th, Katalin; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vtykz-2s695", "eprint_id": 114951, "eprint_status": "archive", "datestamp": "2023-10-13 16:04:16", "lastmod": "2024-01-09 22:19:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Koontz-Alison", "name": { "family": "Koontz", "given": "Alison" } }, { "id": "Urrutia-Hugo-A", "name": { "family": "Urrutia", "given": "Hugo A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." } } ] }, "title": "Retroviral lineage analysis reveals dual contribution from ectodermal placodes and neural crest cells to avian olfactory sensory and GnRH neurons", "ispublished": "pub", "full_text_status": "public", "keywords": "cell lineage analysis; gonadotropin-releasing hormone neurons; neural crest; olfactory; olfactory ensheathing cells; placode; viral labeling; General Medicine", "note": "\u00a9 2022 The Authors. Natural Sciences published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. \n\nIssue Online: 13 July 2022. Version of Record online: 25 May 2022. Manuscript accepted: 19 April 2022. Manuscript revised: 11 April 2022. Manuscript received: 07 July 2021. \n\nAlison Koontz and, Hugo A. Urrutia, and Marianne E. Bronner contributed equally to this work. \n\nThis work was supported by NIH (NIH 5F31DE027583, NIH 5F31DE031154 and NIH R01DE027568). Graphical Abstract was made using a Biorender platform. \n\nAUTHOR CONTRIBUTIONS. AK, HAU, and MEB contributed to the conceptualization, experimentation, and the writing and editing of this paper. AK and HAU performed data collection and analysis, validation, and produced the figures. \n\nThe authors confirm that they have followed the ethical policies of the journal. \n\nCONFLICT OF INTEREST. MEB is a coauthor of the manuscript and an editor of Natural Sciences and was not involved at the handling of the peer-review process of this submission.\n\nSupplemental Material - ntls20210037-sup-0001-figures1.tiff
", "abstract": "The origin of the neurons and glia in the olfactory system of vertebrates has been controversial, with different cell types attributed to being of ectodermal placode versus neural crest lineage, depending upon the species. Here, we use replication incompetent avian retroviruses to perform a prospective cell lineage analysis of either presumptive olfactory placode or neural crest cells during early development of the chick embryo. Surprisingly, the results reveal a dual contribution from both the olfactory placode and neural crest cells to sensory neurons in the nose and gonadotropin-releasing hormone neurons migrating to the olfactory bulb. We also confirm that olfactory ensheathing glia cells are solely derived from the neural crest. Finally, our results show that neural crest cells and olfactory placode cells contribute to p63 positive cells, likely to be basal stem cells of the olfactory epithelium. Taken together, these finding provide evidence for previously unknown contributions of neural crest cells to some cell types in the chick olfactory system and help resolve previous discrepancies in the literature.", "date": "2022-07", "date_type": "published", "publication": "Natural Sciences", "volume": "2", "number": "3", "publisher": "Wiley", "pagerange": "Art. No. e20210037", "id_number": "CaltechAUTHORS:20220526-391453000", "issn": "2698-6248", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220526-391453000", "funders": { "items": [ { "agency": "NIH Predoctoral Fellowship", "grant_number": "5F31DE027583" }, { "agency": "NIHNIH Predoctoral Fellowship", "grant_number": "5F31DE031154" }, { "agency": "NIH", "grant_number": "R01DE027568" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1002/ntls.20210037", "pmcid": "PMC9605686", "primary_object": { "basename": "Natural_Sciences_-_2022_-_Koontz_-_Retroviral_lineage_analysis_reveals_dual_contribution_from_ectodermal_placodes_and.pdf", "url": "https://authors.library.caltech.edu/records/vtykz-2s695/files/Natural_Sciences_-_2022_-_Koontz_-_Retroviral_lineage_analysis_reveals_dual_contribution_from_ectodermal_placodes_and.pdf" }, "related_objects": [ { "basename": "ntls20210037-sup-0001-figures1.tiff", "url": "https://authors.library.caltech.edu/records/vtykz-2s695/files/ntls20210037-sup-0001-figures1.tiff" }, { "basename": "Natural Sciences - 2022 - Koontz - Retroviral lineage analysis reveals dual contribution from ectodermal placodes and.pdf", "url": "https://authors.library.caltech.edu/records/vtykz-2s695/files/Natural Sciences - 2022 - Koontz - Retroviral lineage analysis reveals dual contribution from ectodermal placodes and.pdf" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Koontz, Alison; Urrutia, Hugo A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/z1kye-3e007", "eprint_id": 105301, "eprint_status": "archive", "datestamp": "2023-08-22 15:43:34", "lastmod": "2023-12-22 23:13:19", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Papdogiannis-Vasileios", "name": { "family": "Papdogiannis", "given": "Vasileios" } }, { "id": "Pennati-Alessandro", "name": { "family": "Pennati", "given": "Alessandro" }, "orcid": "0000-0002-2766-2966" }, { "id": "Parker-Hugo-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Rothb\u00e4cher-Ute", "name": { "family": "Rothb\u00e4cher", "given": "Ute" }, "orcid": "0000-0002-9989-6139" }, { "id": "Patthey-Cedric", "name": { "family": "Patthey", "given": "Cedric" }, "orcid": "0000-0002-2627-9578" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Shimeld-Sebastian-M", "name": { "family": "Shimeld", "given": "Sebastian M." }, "orcid": "0000-0003-0195-7536" } ] }, "title": "Hmx gene conservation identifies the origin of vertebrate cranial ganglia", "ispublished": "pub", "full_text_status": "public", "keywords": "Embryology; Evolutionary developmental biology", "note": "\u00a9 2022 Nature Publishing Group. \n\nReceived 14 November 2019; Accepted 07 April 2022; Published 18 May 2022. \n\nWe thank A. Stolfi and R. Zeller for sharing plasmids used in the Ciona CRISPR and overexpression studies, respectively; H. Escriva for hosting C.P. and for access to his amphioxus facility; and S. Green for lamprey husbandry assistance. V.P. was supported by a Natural Motion scholarship. V.P. and S.M.S. acknowledge the Elizabeth Hannah Jenkinson fund for financial support. V.P. also thanks T. Manousaki and C. Tsigenopoulos for their support while based in HCMR. A.P. was supported by the Accademia Nazionale dei Lincei while working in Oxford and by the H2020 Marie Sklodowska-Curie COFUND ARDRE to U.R. while working in Innsbruck. C.P. was supported by an EMBO Long Term Fellowship while working in Oxford. M.E.B. acknowledges support from award R35NS111564 from the NIH. H.J.P. was supported by funds from the Stowers Institute (grant no. 1001). \n\nData availability: Cloned Hmx gene sequences have been deposited in Genbank accessions MN264670\u2013MN264672. RNA-seq data have been deposited in SRA accession GSE141046. Original data underlying Fig. 4b, c of this manuscript can be accessed from the Stowers Original Data Repository at http://odr.stowers.org/websimr/. \n\nContributions: V.P., C.P and S.M.S. conceived the study. V.P. conducted lamprey gene expression analysis, CNE identification, analysis of lamprey reporter gene experiments, Ciona Hmx expression analysis, Ciona Hmx overexpression analysis and RNA-seq and the molecular phylogenetic analyses. A.P. conducted Ciona CNE identification and reporter gene experiments, tests of lamprey CNE activity in Ciona, CRISPR and overexpression reporter analyses, and analysis of Ciona Hmx and Ngn gene expression. C.P. conducted the amphioxus in situ hybridization and participated in RNA-seq data analysis. H.J.P. conducted the lamprey reporter construct injections and analysis. U.R., M.E.B. and S.M.S. supervised the work. All authors contributing to drafting and editing the manuscript. \n\nThe authors declare no competing interests. \n\nPeer review information: Nature thanks Noriyuki Satoh and the other, anonymous reviewers for their contribution to the peer review of this work.\n\nAccepted Version - nihms-1890223.pdf
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", "abstract": "The evolutionary origin of vertebrates included innovations in sensory processing associated with the acquisition of a predatory lifestyle. Vertebrates perceive external stimuli through sensory systems serviced by cranial sensory ganglia, whose neurons arise predominantly from cranial placodes; however, the understanding of the evolutionary origin of placodes and cranial sensory ganglia is hampered by the anatomical differences between living lineages and the difficulty in assigning homology between cell types and structures. Here we show that the homeobox transcription factor Hmx is a constitutive component of vertebrate sensory ganglion development and that in the tunicate Ciona intestinalis, Hmx is necessary and sufficient to drive the differentiation programme of bipolar tail neurons, cells previously thought to be homologues of neural crest. Using Ciona and lamprey transgenesis, we demonstrate that a unique, tandemly duplicated enhancer pair regulated Hmx expression in the stem-vertebrate lineage. We also show notably robust vertebrate Hmx enhancer function in Ciona, demonstrating that deep conservation of the upstream regulatory network spans the evolutionary origin of vertebrates. These experiments demonstrate regulatory and functional conservation between Ciona and vertebrate Hmx, and point to bipolar tail neurons as homologues of cranial sensory ganglia.", "date": "2022-05-26", "date_type": "published", "publication": "Nature", "volume": "605", "number": "7911", "publisher": "Nature Publishing Group", "pagerange": "701-705", "id_number": "CaltechAUTHORS:20200909-144305477", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200909-144305477", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Natural Motion Scholarship" }, { "agency": "Elizabeth Hannah Jenkinson Fund" }, { "agency": "Accademia Nazionale dei Lincei" }, { "agency": "Marie Curie Fellowship" }, { "agency": "European Molecular Biology Organization (EMBO)" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "Stowers Institute", "grant_number": "1001" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1038/s41586-022-04742-w", "pmcid": "PMC10214386", "primary_object": { "basename": "nihms-1890223.pdf", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/nihms-1890223.pdf" }, "related_objects": [ { "basename": "41586_2022_4742_Tab2_ESM.jpg", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Tab2_ESM.jpg" }, { "basename": "41586_2022_4742_Tab3_ESM.jpg", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Tab3_ESM.jpg" }, { "basename": "41586_2022_4742_Fig10_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig10_ESM.webp" }, { "basename": "41586_2022_4742_Fig11_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig11_ESM.webp" }, { "basename": "41586_2022_4742_Fig8_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig8_ESM.webp" }, { "basename": "41586_2022_4742_MOESM4_ESM.zip", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_MOESM4_ESM.zip" }, { "basename": "41586_2022_4742_Fig5_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig5_ESM.webp" }, { "basename": "41586_2022_4742_Fig7_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig7_ESM.webp" }, { "basename": "41586_2022_4742_Fig9_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig9_ESM.webp" }, { "basename": "41586_2022_4742_MOESM2_ESM.docx", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_MOESM2_ESM.docx" }, { "basename": "41586_2022_4742_Fig6_ESM.webp", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Fig6_ESM.webp" }, { "basename": "41586_2022_4742_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_MOESM1_ESM.pdf" }, { "basename": "41586_2022_4742_MOESM3_ESM.docx", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_MOESM3_ESM.docx" }, { "basename": "41586_2022_4742_Tab1_ESM.jpg", "url": "https://authors.library.caltech.edu/records/z1kye-3e007/files/41586_2022_4742_Tab1_ESM.jpg" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Papdogiannis, Vasileios; Pennati, Alessandro; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tyv35-bhb58", "eprint_id": 114672, "eprint_status": "archive", "datestamp": "2023-08-20 07:40:35", "lastmod": "2023-12-22 23:41:46", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Jacobs-Li-Jessica", "name": { "family": "Jacobs-Li", "given": "Jessica" } }, { "id": "Li-Can", "name": { "family": "Li", "given": "Can" }, "orcid": "0000-0002-9301-7850" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Single-cell profiling coupled with lineage analysis reveals distinct sacral neural crest contributions to the developing enteric nervous system", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nThis version posted May 9, 2022. \n\nThis work was supported by R01DE027568 and R35NS111564 to M.E.B. We thank Drs. Igor Antoshechkin and Vijaya Kumar and the Millard and Muriel Jacobs Genetics and Genomics Laboratory at California Institute of Technology for their guidance and support in bulk RNA-sequencing. We thank Jamie Tijerina and Rochelle Diamond from the Beckman Institute Flow Cytometry Facility for their help with the FACS. We thank Dr. Sisi Chen, Jeff Park, Prof. Matt Thomson and SPEC at Caltech for their dedicated support in optimization and guidance in single-cell RNA-sequencing. We thank Dr. Fan Gao and Bioinformatics Resource Center in the Beckman Institute at Caltech for guiding us through single-cell transcriptomic analysis. We appreciate the help from Prof. Carlos Lois for kindly sharing equipment with us to perform RIA concentration. We thank Dr. Michael L. Piacentino, Dr. Erica J. Hutchins and Prof. Angelike Stathopoulos for the helpful discussion on the manuscript. \n\nThe authors have declared no competing interest.\n\nSubmitted - 2022.05.09.491197v1.full.pdf
", "abstract": "During development, the enteric nervous system (ENS) arises from neural crest cells that emerge from the neural tube, migrate to and along the gut, and colonize the entire intestinal tract. While much of the ENS arises from vagal neural crest cells that originate from the caudal hindbrain, there is a second contribution from the sacral neural crest that migrates from the caudal end of the spinal cord to populate the post-umbilical gut. By coupling single cell transcriptomics with axial-level specific lineage tracing in avian embryos, we compared the contributions between embryonic vagal and sacral neural crest cells to the ENS. The results show that the two neural crest populations form partially overlapping but also complementary subsets of neurons and glia in distinct ganglionic units. In particular, the sacral neural crest cells appear to be the major source of adrenergic/dopaminergic and serotonergic neurons, melanocytes and Schwann cells in the post-umbilical gut. In addition to neurons and glia, the results also reveal sacral neural crest contributions to connective tissue and mesenchymal cells of the gut. These findings highlight the specific properties of the sacral neural crest population in the hindgut and have potential implications for understanding development of the complex nervous system in the hindgut environment that may influence congenital neuropathies.", "date": "2022-05-11", "date_type": "published", "id_number": "CaltechAUTHORS:20220511-173943976", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220511-173943976", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R35NS111564" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2022.05.09.491197", "primary_object": { "basename": "2022.05.09.491197v1.full.pdf", "url": "https://authors.library.caltech.edu/records/tyv35-bhb58/files/2022.05.09.491197v1.full.pdf" }, "resource_type": "monograph", "pub_year": "2022", "author_list": "Tang, Weiyi; Jacobs-Li, Jessica; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/98sxc-gy809", "eprint_id": 113678, "eprint_status": "archive", "datestamp": "2023-08-20 07:10:51", "lastmod": "2023-12-13 16:44:36", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lamanna-Francesco", "name": { "family": "Lamanna", "given": "Francesco" }, "orcid": "0000-0002-0447-759X" }, { "id": "Hervas-Sotomayor-Francesca", "name": { "family": "Hervas-Sotomayor", "given": "Francisca" } }, { "id": "Oel-A-Phillip", "name": { "family": "Oel", "given": "A. Phillip" }, "orcid": "0000-0003-4062-0987" }, { "id": "Jandzik-David", "name": { "family": "Jandzik", "given": "David" }, "orcid": "0000-0002-4239-0014" }, { "id": "Sobrido-Came\u00e1n-Daniel", "name": { "family": "Sobrido-Came\u00e1n", "given": "Daniel" }, "orcid": "0000-0001-8239-2965" }, { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Br\u00fcning-Thoomke", "name": { "family": "Br\u00fcning", "given": "Thoomke" } }, { "id": "M\u00f6\u00dfinger-Katharina", "name": { "family": "M\u00f6\u00dfinger", "given": "Katharina" } }, { "id": "Schmidt-Julia", "name": { "family": "Schmidt", "given": "Julia" } }, { "id": "Schneider-Celine", "name": { "family": "Schneider", "given": "Celine" } }, { "id": "Sepp-Mari", "name": { "family": "Sepp", "given": "Mari" }, "orcid": "0000-0003-1733-8385" }, { "id": "Murat-Florent", "name": { "family": "Murat", "given": "Florent" }, "orcid": "0000-0003-2116-2511" }, { "id": "Smith-Jeramiah-J", "name": { "family": "Smith", "given": "Jeramiah J." }, "orcid": "0000-0001-5333-5531" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Rodicio-Mar\u00eda-Celina", "name": { "family": "Rodicio", "given": "Mar\u00eda Celina" }, "orcid": "0000-0002-7267-3285" }, { "id": "Barreiro-Iglesias-Ant\u00f3n", "name": { "family": "Barreiro-Iglesias", "given": "Ant\u00f3n" }, "orcid": "0000-0002-7507-080X" }, { "id": "Medeiros-Daniel-M", "name": { "family": "Medeiros", "given": "Daniel M." }, "orcid": "0000-0002-8182-6028" }, { "id": "Arendt-Detlev", "name": { "family": "Arendt", "given": "Detlev" }, "orcid": "0000-0001-7833-050X" }, { "id": "Kaessmann-Henrik", "name": { "family": "Kaessmann", "given": "Henrik" }, "orcid": "0000-0001-7563-839X" } ] }, "title": "Reconstructing the ancestral vertebrate brain using a lamprey neural cell type atlas", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nWe thank all members of the Kaessmann group for the fruitful discussions; Elisa Panzariello, Marta Sanchez-Delgado, Nils Trost for the brain and animal illustrations, Moritz Mall for providing temporary lab-space and assistance, and Margarida Cardoso-Moreira for discussions and comments on the manuscript. Computations were performed on the Kaessmann lab server (managed by Nils Trost) and the bwForCluster from the Heidelberg University Computational Center (supported by the state of Baden-W\u00fcrttemberg through bwHPC and the German Research Foundation \u2013 INST 35/1134-1 FUGG). This research was supported by grants from the European Research Council (615253, OntoTransEvol), European Commission (Marie Sk\u0142odowska-Curie Actions ITN: EvoCELL), and the Tschira foundation, which funded the Illumina NextSeq machine used for sequencing. DMM and DJ were supported by the grants of National Science Foundation (IOS 1656843), National Institutes of Health/National Institute of Dental and Craniofacial Research (RDE025940), and University of Colorado, Boulder RIO Innovative Seed Grant FY21 (all to DMM). DJ was also supported by the grant from the Scientific Grant Agency of the Slovak Republic (VEGA 1/0450/21). \n\nAuthor contributions: F.L, F.H.-S., and H.K. conceived and organized the study based on H.K.'s original design. F.L, F.H.- S., and H.K wrote the manuscript with input from all authors. F.L. performed all analyses, and developed the brain atlas app. F.H.-S. established and optimized the tissue dissociation protocol, and performed all scRNA-seq and in situ experiments with support from A.P.O., J.S., and C.S., and guidance from M.S. F.L. and F.H.-S. annotated and interpreted the data. T.B. prepared bulk libraries with guidance from K.M. M.S. established the smFISH protocol. F.H.-S., A.P.O., D.J., D.S.C., M.L.M, and S.A.G. collected the samples. A.B.I., D.M.M., M.B., and M.C.R. provided samples. J.J.S. provided early access to genome assemblies and annotations. A.P.O., M.S., F.M., D.S.C., A.B.I., D.M.M., and D.A. provided useful feedback and discussions. H.K. supervised the study and provided funding. \n\nData availability: Raw and processed bulk and single-cell RNA-seq data have been deposited to ArrayExpress with the accession numbers E-MTAB-11085 (bulk) and E-MTAB-11087 (single cell) (https://www.ebi.ac.uk/arrayexpress/). Additional data are available as supplementary information or upon request. Information about gene expression, cell type annotation, and gene orthology relationships across species can be visualized using the online atlas (https://lampreybrain.kaessmannlab.org/). \n\nCode availability: All code underlying the published atlas is available on GitHub (https://github.com/f-lamanna/LampreyBrainAtlas/) together with detailed instructions about its usage. Additional code is available upon request. \n\nThe authors have declared no competing interest.\n\nSubmitted - 2022.02.28.482278v1.full.pdf
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", "abstract": "The vertebrate brain emerged more than ~500 million years ago in common evolutionary ancestors. To systematically trace its cellular and molecular origins, we established a spatially resolved cell type atlas of the entire brain of the sea lamprey - a jawless species whose phylogenetic position affords the reconstruction of ancestral vertebrate traits - based on extensive single-cell RNA-seq and in situ sequencing data. Comparisons of this atlas to neural data from the mouse and other jawed vertebrates unveiled various shared features that enabled the reconstruction of the core cell type composition, tissue structures, and gene expression programs of the ancestral brain. However, our analyses also revealed key tissues and cell types that arose later in evolution. For example, the ancestral vertebrate brain was likely devoid of cerebellar cell types and oligodendrocytes (myelinating cells); our data suggest that the latter emerged from astrocyte-like evolutionary precursors on the jawed vertebrate lineage. Our work illuminates the cellular and molecular architecture of the ancestral vertebrate brain and provides a foundation for exploring its diversification during evolution.", "date": "2022-03-02", "date_type": "published", "publisher": "bioRxiv", "id_number": "CaltechAUTHORS:20220302-893068044", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220302-893068044", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "state of Baden-W\u00fcrttemberg" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "INST 35/1134-1 FUGG" }, { "agency": "European Research Council (ERC)", "grant_number": "615253" }, { "agency": "Marie Curie Fellowship" }, { "agency": "Klaus Tschira Foundation" }, { "agency": "NSF", "grant_number": "IOS-1656843" }, { "agency": "NIH", "grant_number": "RDE025940" }, { "agency": "University of Colorado, Boulder" }, { "agency": "Slovak Research and Development Agency", "grant_number": "VEGA 1/0450/21" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2022.02.28.482278", "primary_object": { "basename": "media-9.zip", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-9.zip" }, "related_objects": [ { "basename": "media-2.csv", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-2.csv" }, { "basename": "media-3.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-3.xlsx" }, { "basename": "media-5.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-5.xlsx" }, { "basename": "media-4.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-4.xlsx" }, { "basename": "media-6.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-6.xlsx" }, { "basename": "media-7.tsv", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-7.tsv" }, { "basename": "media-8.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-8.xlsx" }, { "basename": "2022.02.28.482278v1.full.pdf", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/2022.02.28.482278v1.full.pdf" }, { "basename": "media-1.xlsx", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-1.xlsx" }, { "basename": "media-10.zip", "url": "https://authors.library.caltech.edu/records/98sxc-gy809/files/media-10.zip" } ], "resource_type": "monograph", "pub_year": "2022", "author_list": "Lamanna, Francesco; Hervas-Sotomayor, Francisca; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/16n5x-4nh73", "eprint_id": 114009, "eprint_status": "archive", "datestamp": "2023-08-22 13:57:30", "lastmod": "2023-12-22 23:19:08", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stundl-Jan", "name": { "family": "Stundl", "given": "Jan" }, "orcid": "0000-0002-3740-3378" }, { "id": "Soukup-Vladimir", "name": { "family": "Soukup", "given": "Vladimir" }, "orcid": "0000-0002-1914-283X" }, { "id": "Fran\u011bk-Roman", "name": { "family": "Fran\u011bk", "given": "Roman" }, "orcid": "0000-0002-3464-1872" }, { "id": "Pospisilova-Anna", "name": { "family": "Pospisilova", "given": "Anna" }, "orcid": "0000-0002-8252-0709" }, { "id": "Psutkova-Viktorie", "name": { "family": "Psutkova", "given": "Viktorie" } }, { "id": "P\u0161eni\u010dka-Martin", "name": { "family": "P\u0161eni\u010dka", "given": "Martin" }, "orcid": "0000-0002-3808-7856" }, { "id": "Cerny-Robert", "name": { "family": "Cerny", "given": "Robert" }, "orcid": "0000-0002-0022-0199" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Medeiros-Daniel-M", "name": { "family": "Medeiros", "given": "Daniel Meulemans" }, "orcid": "0000-0002-8182-6028" }, { "id": "Jandzik-David", "name": { "family": "Jandzik", "given": "David" }, "orcid": "0000-0002-4239-0014" } ] }, "title": "Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates", "ispublished": "pub", "full_text_status": "public", "keywords": "CRISPR/Cas9, targeted mutagenesis, non-teleost fish, sturgeon, vertebrates, development, evolution, evo-devo; Cell Biology; Developmental Biology", "note": "\u00a9 2022 Stundl, Soukup, Fran\u011bk, Pospisilova, Psutkova, P\u0161eni\u010dka, Cerny, Bronner, Medeiros and Jandzik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. \n\nReceived: 31 July 2021; Accepted: 17 January 2022; Published: 10 February 2022. \n\nWe thank Marek Rodina, David Gela, Michaela Fu\u010d\u00edkov\u00e1, and Martin Kahanec for their essential help with sterlet spawns; Vojt\u011bch Miller and \u0160t\u011bp\u00e1nka Novotn\u00e1 for technical assistance; Radek \u0160anda for allowing us to use the stereoscopic microscope with Z-stacking. Computational resources were supplied by the Ministry of Education, Youth and Sports of the Czech Republic under the Projects CESNET (Project No. LM2015042) and CERIT-Scientific Cloud (Project No. LM2015085) provided within the program Projects of Large Research, Development and Innovations Infrastructures. D.J. thanks Tyler Square for numerous discussions and priceless advice on everything CRISPR-related and Brent Hawkins for late night fish conversations and insightful comments on the manuscript. \n\nThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2022.750833/full#supplementary-material \n\nThis project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sk\u0142odowska-Curie grant agreement No. 897949 (to JS). VS was supported by Charles University Research Centre program No. 204069, Charles University Grant SVV 260571/202, and the grant of the Czech Science Foundation GACR 18-04580S. The work of RF and MP was supported by the Ministry of Education, Youth and Sports of the Czech Republic, projects CENAKVA (LM2018099), Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370) and Czech Science Foundation (20-23836S). RC was supported by the Czech Science Foundation GACR 19-18634S. MB was supported by National Institutes of Health grant R35NS111564. DM was supported by National Institutes of Health grant NIDCR R21 RDE025940A and National Science Foundation grant IOS 1656843. DJ was supported by the European Union's Horizon 2020 research and innovation programme under the Marie Sk\u0142odowska-Curie grant agreement no. 751066 and by the Scientific Grant Agency of the Slovak Republic VEGA grant no. 1/0450/21. \n\nAuthor Contributions. DJ conceived and designed the project, JS, VS, RF, AP, VP, MP, and DJ performed the experiments, JS and DJ collected and interpreted the data, DJ, DM, and JS wrote the manuscript. All authors discussed the results and contributed to the final manuscript. \n\nData Availability Statement. The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author. \n\nThe animal study was reviewed and approved by The Animal Research Committee of Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Vod\u0148any, Czech Republic and Ministry of Agriculture of the Czech Republic (MSMT-12550/2016-3). \n\nAuthor Contributions. DJ conceived and designed the project, JS, VS, RF, AP, VP, MP, and DJ performed the experiments, JS and DJ collected and interpreted the data, DJ, DM, and JS wrote the manuscript. All authors discussed the results and contributed to the final manuscript.\n\nPublished - fcell-10-750833.pdf
Supplemental Material - DataSheet1_Efficient_CRISPR_Mutagenesis_in_Sturgeon_Demonstrates_Its_Utility_in_Large,_Slow-Maturing_Vertebrates.pdf
", "abstract": "In the last decade, the CRISPR/Cas9 bacterial virus defense system has been adapted as a user-friendly, efficient, and precise method for targeted mutagenesis in eukaryotes. Though CRISPR/Cas9 has proven effective in a diverse range of organisms, it is still most often used to create mutant lines in lab-reared genetic model systems. However, one major advantage of CRISPR/Cas9 mutagenesis over previous gene targeting approaches is that its high efficiency allows the immediate generation of near-null mosaic mutants. This feature could potentially allow genotype to be linked to phenotype in organisms with life histories that preclude the establishment of purebred genetic lines; a group that includes the vast majority of vertebrate species. Of particular interest to scholars of early vertebrate evolution are several long-lived and slow-maturing fishes that diverged from two dominant modern lineages, teleosts and tetrapods, in the Ordovician, or before. These early-diverging or \"basal\" vertebrates include the jawless cyclostomes, cartilaginous fishes, and various non-teleost ray-finned fishes. In addition to occupying critical phylogenetic positions, these groups possess combinations of derived and ancestral features not seen in conventional model vertebrates, and thus provide an opportunity for understanding the genetic bases of such traits. Here we report successful use of CRISPR/Cas9 mutagenesis in one such non-teleost fish, sterlet Acipenser ruthenus, a small species of sturgeon. We introduced mutations into the genes Tyrosinase, which is needed for melanin production, and Sonic hedgehog, a pleiotropic developmental regulator with diverse roles in early embryonic patterning and organogenesis. We observed disruption of both loci and the production of consistent phenotypes, including both near-null mutants' various hypomorphs. Based on these results, and previous work in lamprey and amphibians, we discuss how CRISPR/Cas9 F0 mutagenesis may be successfully adapted to other long-lived, slow-maturing aquatic vertebrates and identify the ease of obtaining and injecting eggs and/or zygotes as the main challenges.", "date": "2022-02-10", "date_type": "published", "publication": "Frontiers in Cell and Developmental Biology", "volume": "10", "publisher": "Frontiers Media SA", "pagerange": "Art. No. 750833", "id_number": "CaltechAUTHORS:20220322-742455000", "issn": "2296-634X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20220322-742455000", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Marie Curie Fellowship", "grant_number": "897949" }, { "agency": "Charles University", "grant_number": "204069" }, { "agency": "Charles University", "grant_number": "SVV 260571/202" }, { "agency": "Czech Science Foundation", "grant_number": "GACR 18-04580S" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "LM2018099" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "CZ.02.1.01/0.0/0.0/16_025/0007370" }, { "agency": "Czech Science Foundation", "grant_number": "20-23836S" }, { "agency": "Czech Science Foundation", "grant_number": "GACR 19-18634S" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NIH", "grant_number": "R21 RDE025940A" }, { "agency": "NSF", "grant_number": "IOS-1656843" }, { "agency": "Marie Curie Fellowship", "grant_number": "751066" }, { "agency": "Scientific Grant Agency (Slovakia)", "grant_number": "1/0450/21" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "LM2015042" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "LM2015085" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.3389/fcell.2022.750833", "pmcid": "PMC8867083", "primary_object": { "basename": "DataSheet1_Efficient_CRISPR_Mutagenesis_in_Sturgeon_Demonstrates_Its_Utility_in_Large,_Slow-Maturing_Vertebrates.pdf", "url": "https://authors.library.caltech.edu/records/16n5x-4nh73/files/DataSheet1_Efficient_CRISPR_Mutagenesis_in_Sturgeon_Demonstrates_Its_Utility_in_Large,_Slow-Maturing_Vertebrates.pdf" }, "related_objects": [ { "basename": "fcell-10-750833.pdf", "url": "https://authors.library.caltech.edu/records/16n5x-4nh73/files/fcell-10-750833.pdf" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Stundl, Jan; Soukup, Vladimir; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nag4c-etd35", "eprint_id": 112603, "eprint_status": "archive", "datestamp": "2023-08-22 13:35:57", "lastmod": "2023-12-22 23:19:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "El-Nachef-Wael-N", "name": { "family": "El-Nachef", "given": "Wael N." }, "orcid": "0000-0002-2892-9394" }, { "id": "Hu-Claire", "name": { "family": "Hu", "given": "Claire" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Whole gut imaging allows quantification of all enteric neurons in the adult zebrafish intestine", "ispublished": "pub", "full_text_status": "public", "keywords": "enteric nervous system; enteric neuropathy; optical transparency; Gastroenterology; Endocrine and Autonomic Systems; Physiology", "note": "\u00a9 2021 John Wiley & Sons Ltd. \n\nIssue Online: 28 January 2022; Version of Record online: 04 December 2021; Manuscript accepted: 27 October 2021; Manuscript revised: 22 July 2021; Manuscript received: 20 January 2021. \n\nThis work was supported by the National Institutes of Health (NIH R35NS111564 to M.E.B., NIH R01NS108500 to M.E.B., and NIH K08DK123387 to WNE).\n\nAccepted Version - nihms-1762454.pdf
", "abstract": "Background: A fundamental understanding of the enteric nervous system in normal and diseased states is limited by the lack of standard measures of total enteric neuron number. The adult zebrafish is a useful model in this context as it is amenable to in toto imaging of the intestine. We leveraged this to develop a technique to image and quantify all enteric neurons within the adult zebrafish intestine and applied this method to assess the relationship between intestinal length and total enteric neuron number. \n\nMethods: Dissected adult zebrafish intestines were immunostained in wholemount, optically cleared with refractive index-matched solution, and then imaged in tiles using light-sheet microscopy. Imaging software was used to stitch the tiles, and the full image underwent automated cell counting. Total enteric neuron number was assessed in relation to intestinal length using linear regression modeling. \n\nKey Results: Whole gut imaging of the adult zebrafish intestine permits the visualization of endogenous and immunohistochemistry-derived fluorescence throughout the intestine. While enteric neuron distribution is heterogeneous between intestinal segments, total enteric neuron number positively correlates with intestinal length. \n\nConclusions & Inferences: Imaging of all enteric neurons within the adult vertebrate intestine is possible in models such as the zebrafish. In this study, we apply this to demonstrate a positive correlation between enteric neuron number and intestinal length. Quantifying total enteric numbers will facilitate future studies of enteric neuropathies and ENS structure in animal models and potentially in biopsied tissue samples.", "date": "2022-02", "date_type": "published", "publication": "Neurogastroenterology and Motility", "volume": "34", "number": "2", "publisher": "Wiley", "pagerange": "Art. No. e14292", "id_number": "CaltechAUTHORS:20211221-619103000", "issn": "1350-1925", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20211221-619103000", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NIH", "grant_number": "R01NS108500" }, { "agency": "NIH", "grant_number": "K08DK123387" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1111/nmo.14292", "pmcid": "PMC8799505", "primary_object": { "basename": "nihms-1762454.pdf", "url": "https://authors.library.caltech.edu/records/nag4c-etd35/files/nihms-1762454.pdf" }, "resource_type": "article", "pub_year": "2022", "author_list": "El-Nachef, Wael N.; Hu, Claire; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j6qbg-4x283", "eprint_id": 110191, "eprint_status": "archive", "datestamp": "2023-08-20 06:51:20", "lastmod": "2023-12-22 23:22:54", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Williams-Ruth-M", "name": { "family": "Williams", "given": "Ruth M." }, "orcid": "0000-0002-2628-7834" }, { "id": "Lukoseviciute-Martyna", "name": { "family": "Lukoseviciute", "given": "Martyna" }, "orcid": "0000-0002-8915-5249" }, { "id": "Sauka-Spengler-Tatjana", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Single-cell atlas of early chick development reveals gradual segregation of neural crest lineage from the neural plate border during neurulation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2022 Williams et. al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. \n\nPreprinted: 08 August 2021; Received: 05 October 2021; Accepted: 01 December 2021; Published: 28 January 2022. \n\nFluorescence activated cell sorting was performed at California Institute of Technology Flow Cytometry Facility using BD Biosciences FACSAria Cell Sorter with Patrick Cannon. 10 X libraries were prepared in the Thomson lab at California Institute of Technology with assistance from Jeff Park. Illumina sequencing was performed at the Millard and Muriel Jacob at California Institute of Technology with Igor Antoshechkin. HH4 10 X library was constructed at MRC Weatherall Institute of Molecular Medicine, University of Oxford with assistance from Kevin Clark (FACS facility), Dr Neil Ashley (single-cell facility) and Tim Rostron (NGS sequencing facility). Confocal microscopy was performed within the Biological Imaging Facility at the Beckman Institute, California Institute of Technology with assistance from Dr Giada Spigolon. We thank members of the Bronner and Sauka-Spengler labs for their support and helpful discussions. This work was funded by NIH R01DE027538 to MEB/RMW, Wellcome Trust Senior Research Fellowship (215615/Z/19/Z) to TSS/RMW and Radcliffe Department of Medicine Scholarship and MRC DTP Supplementary Funding to ML. \n\nAuthor contributions: Ruth M Williams, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing \u2013 original draft; Martyna Lukoseviciute, Formal analysis, Writing \u2013 review and editing; Tatjana Sauka-Spengler, Formal analysis, Funding acquisition, Supervision, Writing \u2013 review and editing; Marianne E Bronner, Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing \u2013 review and editing. \n\nCompeting interests: Marianne E Bronner: Senior editor, eLife. The other authors declare that no competing interests exist. \n\nData availability: Sequencing data have been deposited in GEO under accession codes GSE181577.\n\nPublished - elife-74464-v1.pdf
Submitted - 2021.08.08.455573v1.full.pdf
Supplemental Material - elife-74464-supp1-v1.xlsx
Supplemental Material - elife-74464-transrepform1-v1.docx
", "abstract": "The epiblast of vertebrate embryos is comprised of neural and non-neural ectoderm, with the border territory at their intersection harboring neural crest and cranial placode progenitors. Here, we a generate single-cell atlas of the developing chick epiblast from late gastrulation through early neurulation stages to define transcriptional changes in the emerging 'neural plate border' as well as other regions of the epiblast. Focusing on the border territory, the results reveal gradual establishment of heterogeneous neural plate border signatures, including novel genes that we validate by fluorescent in situ hybridization. Developmental trajectory analysis infers that segregation of neural plate border lineages only commences at early neurulation, rather than at gastrulation as previously predicted. We find that cells expressing the prospective neural crest marker Pax7 contribute to multiple lineages, and a subset of premigratory neural crest cells shares a transcriptional signature with their border precursors. Together, our results suggest that cells at the neural plate border remain heterogeneous until early neurulation, at which time progenitors become progressively allocated toward defined neural crest and placode lineages. The data also can be mined to reveal changes throughout the developing epiblast.", "date": "2022-01-28", "date_type": "published", "publication": "eLife", "volume": "11", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e74464", "id_number": "CaltechAUTHORS:20210810-170230749", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210810-170230749", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "Wellcome Trust", "grant_number": "215615/Z/19/Z" }, { "agency": "Radcliffe Department of Medicine" }, { "agency": "Medical Research Council (UK)" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.7554/eLife.74464", "pmcid": "PMC8798042", "primary_object": { "basename": "elife-74464-v1.pdf", "url": "https://authors.library.caltech.edu/records/j6qbg-4x283/files/elife-74464-v1.pdf" }, "related_objects": [ { "basename": "2021.08.08.455573v1.full.pdf", "url": "https://authors.library.caltech.edu/records/j6qbg-4x283/files/2021.08.08.455573v1.full.pdf" }, { "basename": "elife-74464-supp1-v1.xlsx", "url": "https://authors.library.caltech.edu/records/j6qbg-4x283/files/elife-74464-supp1-v1.xlsx" }, { "basename": "elife-74464-transrepform1-v1.docx", "url": "https://authors.library.caltech.edu/records/j6qbg-4x283/files/elife-74464-transrepform1-v1.docx" } ], "resource_type": "article", "pub_year": "2022", "author_list": "Williams, Ruth M.; Lukoseviciute, Martyna; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wtm76-b5b60", "eprint_id": 110822, "eprint_status": "archive", "datestamp": "2023-08-22 12:27:31", "lastmod": "2023-12-22 23:13:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Solovieva-Tatiana", "name": { "family": "Solovieva", "given": "Tatiana" }, "orcid": "0000-0001-6194-2550" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Reprint of: Schwann cell precursors: Where they come from and where they go", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Schwann cell precursor; Peripheral nervous system; Cell lineage; Neural crest", "note": "\u00a9 2021 Elsevier B.V. \n\nReceived 28 February 2021, Revised 29 April 2021, Accepted 30 April 2021, Available online 26 August 2021. \n\nA publisher's error resulted in this article appearing in the wrong issue. The article is reprinted here for the reader's convenience and for the continuity of the special issue. \n\nThis work was supported by grants from the National Institutes of Health (R01DE027568 and R35NS111564). \n\nCRediT authorship contribution statement. Tatiana Solovieva and Marianne Bronner wrote the manuscript. \n\nThere are no competing interests.", "abstract": "Schwann cell precursors (SCPs) are a transient population in the embryo, closely associated with nerves along which they migrate into the periphery of the body. Long considered to be progenitors that only form Schwann cells\u2014the myelinating cells of nerves, current evidence suggests that SCPs have much broader developmental potential. Indeed, different cell marking techniques employed over the past 20 years have identified multiple novel SCP derivatives throughout the body. It is now clear that SCPs represent a multipotent progenitor population, which also display a level of plasticity in response to injury. Moreover, they originate from multiple origins in the embryo and may reflect several distinct subpopulations in terms of molecular identity and fate. Here we review SCP origins, derivatives and plasticity in development, growth and repair.", "date": "2021-12", "date_type": "published", "publication": "Cells & Development", "volume": "168", "publisher": "Elsevier", "pagerange": "Art. No. 203729", "id_number": "CaltechAUTHORS:20210913-185436793", "issn": "2667-2901", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210913-185436793", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R35NS111564" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.cdev.2021.203729", "resource_type": "article", "pub_year": "2021", "author_list": "Solovieva, Tatiana and Bronner, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/56qk1-2zh48", "eprint_id": 110173, "eprint_status": "archive", "datestamp": "2023-08-22 11:43:40", "lastmod": "2023-12-22 23:22:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-Hugo-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "De-Kumar-Bony", "name": { "family": "De Kumar", "given": "Bony" }, "orcid": "0000-0001-8398-3775" }, { "id": "Pushel-Irina", "name": { "family": "Pushel", "given": "Irina" }, "orcid": "0000-0002-0862-1923" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-Robb", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" } ] }, "title": "Analysis of lamprey meis genes reveals that conserved inputs from Hox, Meis and Pbx proteins control their expression in the hindbrain and neural tube", "ispublished": "pub", "full_text_status": "public", "keywords": "Meis genes; Hox genes; Lamprey; Hindbrain segmentation; Neural crest cells; Gene regulatory networks; cis-Regulatory elements; Vertebrate evolution; Head development", "note": "\u00a9 2021 Elsevier Inc. \n\nReceived 15 January 2021, Revised 10 June 2021, Accepted 22 July 2021, Available online 24 July 2021. \n\nWe thank Stephen Green, Dorit Hockman, Tetsuto Miyashita, and Megan Martik for lamprey husbandry assistance, the Stowers Institute Histology facility for sectioning assistance and the aquatics facility for help in maintenance of zebrafish. HJP, BDK and RK were supported by funds from the Stowers Institute (RK grant #2020\u20131001). MEB was supported by NIH grant R35 NS111564. This work was performed to fulfill, in part, requirements for IP's thesis research in the Graduate School of the Stowers Institute for Medical Research.\n\nAccepted Version - 1-s2.0-S0012160621001767-main_acc.pdf
", "abstract": "Meis genes are known to play important roles in the hindbrain and neural crest cells of jawed vertebrates. To explore the roles of Meis genes in head development during evolution of vertebrates, we have identified four meis genes in the sea lamprey genome and characterized their patterns of expression and regulation, with a focus on the hindbrain and pharynx. Each of the lamprey meis genes displays temporally and spatially dynamic patterns of expression, some of which are coupled to rhombomeric domains in the developing hindbrain and select pharyngeal arches. Studies of Meis loci in mouse and zebrafish have identified enhancers that are bound by Hox and TALE (Meis and Pbx) proteins, implicating these factors in the direct regulation of Meis expression. We examined the lamprey meis loci and identified a series of cis-elements conserved between lamprey and jawed vertebrate meis genes. In transgenic reporter assays we demonstrated that these elements act as neural enhancers in lamprey embryos, directing reporter expression in appropriate domains when compared to expression of their associated endogenous meis gene. Sequence alignments reveal that these conserved elements are in similar relative positions of the meis loci and contain a series of consensus binding motifs for Hox and TALE proteins. This suggests that ancient Hox and TALE-responsive enhancers regulated expression of ancestral vertebrate meis genes in segmental domains in the hindbrain and have been retained in the meis loci during vertebrate evolution. The presence of conserved Meis, Pbx and Hox binding sites in these lamprey enhancers links Hox and TALE factors to regulation of lamprey meis genes in the developing hindbrain, indicating a deep ancestry for these regulatory interactions prior to the divergence of jawed and jawless vertebrates.", "date": "2021-11", "date_type": "published", "publication": "Developmental Biology", "volume": "479", "publisher": "Elsevier", "pagerange": "61-76", "id_number": "CaltechAUTHORS:20210809-181055002", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210809-181055002", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Stowers Institute for Medical Research", "grant_number": "2020-1001" }, { "agency": "NIH", "grant_number": "R35 NS111564" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.ydbio.2021.07.014", "primary_object": { "basename": "1-s2.0-S0012160621001767-main_acc.pdf", "url": "https://authors.library.caltech.edu/records/56qk1-2zh48/files/1-s2.0-S0012160621001767-main_acc.pdf" }, "resource_type": "article", "pub_year": "2021", "author_list": "Parker, Hugo J.; De Kumar, Bony; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0pqwg-x8c94", "eprint_id": 110982, "eprint_status": "archive", "datestamp": "2023-08-20 05:47:35", "lastmod": "2023-12-22 23:13:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Seq Your Destiny: Neural Crest Fate Determination in the Genomic Era", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, cell fate, developmental potential, genomics, specification, neural plate border", "note": "\u00a9 2021 Annual Reviews. \n\nReview in Advance first posted online on September 21, 2021.", "abstract": "Neural crest stem/progenitor cells arise early during vertebrate embryogenesis at the border of the forming central nervous system. They subsequently migrate throughout the body, eventually differentiating into diverse cell types ranging from neurons and glia of the peripheral nervous system to bones of the face, portions of the heart, and pigmentation of the skin. Along the body axis, the neural crest is heterogeneous, with different subpopulations arising in the head, neck, trunk, and tail regions, each characterized by distinct migratory patterns and developmental potential. Modern genomic approaches like single-cell RNA- and ATAC-sequencing (seq) have greatly enhanced our understanding of cell lineage trajectories and gene regulatory circuitry underlying the developmental progression of neural crest cells. Here, we discuss how genomic approaches have provided new insights into old questions in neural crest biology by elucidating transcriptional and posttranscriptional mechanisms that govern neural crest formation and the establishment of axial level identity.", "date": "2021-11", "date_type": "published", "publication": "Annual Review of Genetics", "volume": "55", "publisher": "Annual Reviews", "pagerange": "349-376", "id_number": "CaltechAUTHORS:20210922-140122706", "issn": "0066-4197", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210922-140122706", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1146/annurev-genet-071719-020954", "resource_type": "article", "pub_year": "2021", "author_list": "Gandhi, Shashank and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fm97x-6g024", "eprint_id": 110692, "eprint_status": "archive", "datestamp": "2023-08-22 11:24:14", "lastmod": "2023-12-22 23:14:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Riding the crest to get a head: neural crest evolution in vertebrates", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Development of the nervous system; Developmental biology; Evolution", "note": "\u00a9 2021 Nature Publishing Group. \n\nAccepted 13 July 2021; Published 01 September 2021. \n\nThe authors thank J. Stundl for comments and discussion on this manuscript. This work was supported by the US National Institutes of Health (NIH) grant R35NS111564 to M.E.B. M.L.M. was supported by a fellowship from the Helen Hay Whitney Foundation and by NIH grant 1K99HD100587. \n\nAuthor Contributions: The authors contributed equally to all aspects of the article. \n\nThe authors declare no competing interests. \n\nPeer review information: Nature Reviews Neuroscience thanks I. Adameyko, M. Levine and A. Monsoro-Burq for their contribution to the peer review of this work.", "abstract": "In their seminal 1983 paper, Gans and Northcutt proposed that evolution of the vertebrate 'new head' was made possible by the advent of the neural crest and cranial placodes. The neural crest is a stem cell population that arises adjacent to the forming CNS and contributes to important cell types, including components of the peripheral nervous system and craniofacial skeleton and elements of the cardiovascular system. In the past few years, the new head hypothesis has been challenged by the discovery in invertebrate chordates of cells with some, but not all, characteristics of vertebrate neural crest cells. Here, we discuss recent findings regarding how neural crest cells may have evolved during the course of deuterostome evolution. The results suggest that there was progressive addition of cell types to the repertoire of neural crest derivatives throughout vertebrate evolution. Novel genomic tools have enabled higher resolution insight into neural crest evolution, from both a cellular and a gene regulatory perspective. Together, these data provide clues regarding the ancestral neural crest state and how the neural crest continues to evolve to contribute to the success of vertebrates as efficient predators.", "date": "2021-10", "date_type": "published", "publication": "Nature Reviews. Neuroscience", "volume": "22", "number": "10", "publisher": "Nature Publishing Group", "pagerange": "616-626", "id_number": "CaltechAUTHORS:20210901-191523412", "issn": "1471-003X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210901-191523412", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "Helen Hay Whitney Foundation" }, { "agency": "NIH", "grant_number": "1K99HD100587" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1038/s41583-021-00503-2", "resource_type": "article", "pub_year": "2021", "author_list": "Martik, Megan L. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dqbh1-ew965", "eprint_id": 109649, "eprint_status": "archive", "datestamp": "2023-08-22 11:00:28", "lastmod": "2023-12-22 23:22:58", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Essential function and targets of BMP signaling during midbrain neural crest delamination", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; Epithelial-to-mesenchymal transition; BMP signaling; RNA Seq; Delamination; Migration", "note": "\u00a9 2021 Elsevier Inc. \n\nReceived 31 March 2021, Revised 27 May 2021, Accepted 1 June 2021, Available online 6 June 2021.\n\nWe would like to thank Megan Martik and Shashank Gandhi for valuable discussion on experiment design and analysis, and Gabriel da Silva Pescador for technical support. We thank Elisa Mart\u00ed for sharing reagents, Patrick Cannon and Rochelle Diamond at the Caltech Flow Cytometry Cell Sorting Facility for cell sorting, Igor Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for library sequencing, and the Beckman Institute Biological Imaging Facility for microscopy support. Funding for this work comes from the National Institutes of Health grants K99DE029240 to M.L.P., K99DE028592 to E.J.H, R01DE027538 and R01DE027568 to M.E.B.\n\nAuthor contributions:\nConceptualization: M.L.P., E.J.H., and M.E.B.; Experiment design: M.L.P. and E.J.H.; Experimentation: M.L.P. and E.J.H.; Data analysis: M.L.P.; Data interpretation: M.L.P., E.J.H., and M.E.B.; Manuscript preparation: M.L.P.; Manuscript editing: M.E.B. and E.J.H.\n\nData and code availability:\nAll source data and associated code used for analysis are publicly available on GitHub at https://github.com/mpiacentino/Transcriptome-profiling-reveals-BMP-target-genes-during-midbrain-neural-crest-delamination. Raw RNA-seq results are available in the following NCBI BioProjects: Control replicates BioProject #PRJNA673315 (performed in collaboration with (Hutchins et al., 2021)), and dnBMPR1A-FLAG replicates BioProject #PRJNA717985.\n\nThe authors declare no competing interests.", "abstract": "BMP signaling plays iterative roles during vertebrate neural crest development from induction through craniofacial morphogenesis. However, far less is known about the role of BMP activity in cranial neural crest epithelial-to-mesenchymal transition and delamination. By measuring canonical BMP signaling activity as a function of time from specification through early migration of avian midbrain neural crest cells, we found elevated BMP signaling during delamination stages. Moreover, inhibition of canonical BMP activity via a dominant negative mutant Type I BMP receptor showed that BMP signaling is required for neural crest migration from the midbrain, independent from an effect on EMT and delamination. Transcriptome profiling on control compared to BMP-inhibited cranial neural crest cells identified novel BMP targets during neural crest delamination and early migration including targets of the Notch pathway that are upregulated following BMP inhibition. These results suggest potential crosstalk between the BMP and Notch pathways in early migrating cranial neural crest and provide novel insight into mechanisms regulated by BMP signaling during early craniofacial development.", "date": "2021-09", "date_type": "published", "publication": "Developmental Biology", "volume": "477", "publisher": "Elsevier", "pagerange": "251-261", "id_number": "CaltechAUTHORS:20210629-192923094", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210629-192923094", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.ydbio.2021.06.003", "resource_type": "article", "pub_year": "2021", "author_list": "Piacentino, Michael L.; Hutchins, Erica J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jqhq2-0q756", "eprint_id": 110030, "eprint_status": "archive", "datestamp": "2023-08-20 04:20:20", "lastmod": "2023-12-13 16:44:29", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Zhang-Haifeng", "name": { "family": "Zhang", "given": "Haifeng" } }, { "name": { "family": "Shang", "given": "Renjie" } }, { "name": { "family": "Kim", "given": "Kwantae" } }, { "name": { "family": "Zheng", "given": "Wei" } }, { "name": { "family": "Johnson", "given": "Christopher J." } }, { "name": { "family": "Sun", "given": "Lei" } }, { "name": { "family": "Niu", "given": "Xiang" } }, { "name": { "family": "Liu", "given": "Liang" } }, { "name": { "family": "Uyeno", "given": "Theodore A." } }, { "name": { "family": "Zhou", "given": "Jingqi" } }, { "name": { "family": "Liu", "given": "Lingshu" } }, { "name": { "family": "Pei", "given": "Jimin" } }, { "name": { "family": "Fissette", "given": "Skye D." } }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "name": { "family": "Samudra", "given": "Sukhada P." } }, { "name": { "family": "Wen", "given": "Junfei" } }, { "name": { "family": "Zhang", "given": "Jianli" } }, { "name": { "family": "Eggenschwiler", "given": "Jonathan" } }, { "name": { "family": "Menke", "given": "Doug" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "name": { "family": "Grishin", "given": "Nick V." } }, { "name": { "family": "Li", "given": "Weiming" } }, { "name": { "family": "Ye", "given": "Kaixiong" } }, { "name": { "family": "Zhang", "given": "Yang" } }, { "name": { "family": "Stolfi", "given": "Alberto" } }, { "name": { "family": "Bi", "given": "Pengpeng" }, "orcid": "0000-0002-9871-6773" } ] }, "title": "Evolution of a chordate-specific mechanism for myoblast fusion", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nThis version posted July 25, 2021. \n\nWe thank trainees G. Gopu, A. Baiju, and E. M. Hicks in Bi laboratory and A. L. Womble from Valdosta State University for technical help. We are grateful to E. N. Olson from University of Texas Southwestern Medical Center for critical reading of the manuscript. We thank H. Li from Ocean University of China, C. Ca\u00f1estro from University of Barcelona, S. Kuraku, R. Kusakabe and S. Kuratani from RIKEN, M. Cui from University of Texas Southwestern Medical Center, S. Du from University of Maryland School of Medicine, J. Ziermann from Howard University, Z. Yang from University of College London, Michael Coates and Tetsuto Miyashita from University of Chicago, and F. Razy-Krajka for advice; A. Bigot and V. Mouly from the Myoline platform of the Myology Institute for myoblast cell lines; X. Li from University of Texas Southwestern Medical Center, N. S. Johnson from United States Geological Survey, M. Brindley from University of Georgia for providing materials and reagents. \n\nThis work was supported by the starting up fund from the University of Georgia to P.B., NIH R00 award HD084814 and NSF award 1940743 to A.S., an NSF Graduate Research Fellowship to C.J.J., Great Lakes Fishery Commission (540810) to S.D.F. and W.L., and NSF award 1354788 to T.A.U. \n\nAuthor contributions: H.Z., R.S., A.S., P.B. designed research; H.Z., R.S., K.K., W.Z., C.J.J., S.L., X.N., L.L., T.A.U., J.Z., L.L., J.P., S.D.F., S.A.G., S.P.S., J.W., J.Z., J.E., D.M., M.E.B., N.V.G., W.L., K.Y., Z.Y., A.S. and P.B. performed research; H.Z., R.S., K.K., W.Z., C.J.J., L.S., X.N., L.L., J.Z., L.L., J.W., W.L., K.Y., Z.Y., A.S. and P.B. analyzed data; A.S. and P.B. wrote the paper. \n\nThe authors declare that they have no competing interests. \n\nData and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.\n\nSubmitted - 2021.07.24.453587v1.full.pdf
Supplemental Material - media-1.pdf
Supplemental Material - media-2.xlsx
Supplemental Material - media-3.zip
Supplemental Material - media-4.zip
Supplemental Material - media-5.pdf
Supplemental Material - media-6.pdf
Supplemental Material - media-7.zip
", "abstract": "The size of an animal is determined by the size of its musculoskeletal system. Myoblast fusion is an innovative mechanism that allows for multinucleated muscle fibers to compound the size and strength of individual mononucleated cells. However, the evolutionary history of the control mechanism underlying this important process is currently unknown. The phylum Chordata hosts closely related groups that span distinct myoblast fusion states: no fusion in cephalochordates, restricted fusion and multinucleation in tunicates, and extensive, obligatory fusion in vertebrates. To elucidate how these differences may have evolved, we studied the evolutionary origins and function of membrane-coalescing agents Myomaker and Myomixer in various groups of chordates. Here we report that Myomaker likely arose through gene duplication in the last common ancestor of tunicates and vertebrates, while Myomixer appears to have evolved de novo in early vertebrates. Functional tests revealed an unexpectedly complex evolutionary history of myoblast fusion in chordates. A pre-vertebrate phase of muscle multinucleation driven by Myomaker was followed by the later emergence of Myomixer that enables the highly efficient fusion system of vertebrates. Thus, our findings reveal the evolutionary origins of chordate-specific fusogens and illustrate how new genes can shape the emergence of novel morphogenetic traits and mechanisms.", "date": "2021-07-28", "date_type": "published", "id_number": "CaltechAUTHORS:20210727-173554020", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210727-173554020", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "University of Georgia" }, { "agency": "NIH", "grant_number": "HD084814" }, { "agency": "NSF", "grant_number": "IOS-1940743" }, { "agency": "NSF Graduate Research Fellowship" }, { "agency": "Great Lakes Fishery Commission", "grant_number": "540810" }, { "agency": "NSF", "grant_number": "IOS-1354788" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2021.07.24.453587", "primary_object": { "basename": "media-3.zip", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-3.zip" }, "related_objects": [ { "basename": "media-4.zip", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-4.zip" }, { "basename": "media-5.pdf", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-5.pdf" }, { "basename": "media-6.pdf", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-6.pdf" }, { "basename": "media-7.zip", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-7.zip" }, { "basename": "2021.07.24.453587v1.full.pdf", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/2021.07.24.453587v1.full.pdf" }, { "basename": "media-1.pdf", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-1.pdf" }, { "basename": "media-2.xlsx", "url": "https://authors.library.caltech.edu/records/jqhq2-0q756/files/media-2.xlsx" } ], "resource_type": "monograph", "pub_year": "2021", "author_list": "Zhang, Haifeng; Shang, Renjie; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/d9ytj-8vd79", "eprint_id": 109314, "eprint_status": "archive", "datestamp": "2023-08-22 10:03:38", "lastmod": "2023-12-22 23:13:12", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Solovieva-Tatiana", "name": { "family": "Solovieva", "given": "Tatiana" }, "orcid": "0000-0001-6194-2550" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Schwann cell precursors: Where they come from and where they go", "ispublished": "pub", "full_text_status": "public", "keywords": "Schwann cell precursor; Peripheral nervous system; Cell lineage; Neural crest", "note": "\u00a9 2021 Elsevier. \n\nReceived 28 February 2021, Revised 29 April 2021, Accepted 30 April 2021, Available online 3 May 2021. \n\nThis work was supported by grants from the National Institutes of Health (R01DE027568 and R35NS111564). \n\nCRediT authorship contribution statement. Tatiana Solovieva and Marianne Bronner wrote the manuscript. \n\nThere are no competing interests.\n\nAccepted Version - nihms-1703472.pdf
", "abstract": "Schwann cell precursors (SCPs) are a transient population in the embryo, closely associated with nerves along which they migrate into the periphery of the body. Long considered to be progenitors that only form Schwann cells\u2014the myelinating cells of nerves, current evidence suggests that SCPs have much broader developmental potential. Indeed, different cell marking techniques employed over the past 20 years have identified multiple novel SCP derivatives throughout the body. It is now clear that SCPs represent a multipotent progenitor population, which also display a level of plasticity in response to injury. Moreover, they originate from multiple origins in the embryo and may reflect several distinct subpopulations in terms of molecular identity and fate. Here we review SCP origins, derivatives and plasticity in development, growth and repair.", "date": "2021-06", "date_type": "published", "publication": "Cells & Development", "volume": "166", "publisher": "Elsevier", "pagerange": "Art. No. 203686", "id_number": "CaltechAUTHORS:20210528-155102352", "issn": "2667-2901", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210528-155102352", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R35NS111564" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.cdev.2021.203686", "pmcid": "PMC8496487", "primary_object": { "basename": "nihms-1703472.pdf", "url": "https://authors.library.caltech.edu/records/d9ytj-8vd79/files/nihms-1703472.pdf" }, "resource_type": "article", "pub_year": "2021", "author_list": "Solovieva, Tatiana and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zx2p9-8d270", "eprint_id": 108963, "eprint_status": "archive", "datestamp": "2023-08-20 02:52:14", "lastmod": "2023-12-13 16:49:58", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Galton-Riley", "name": { "family": "Galton", "given": "Riley" }, "orcid": "0000-0001-6777-2177" }, { "id": "Fejes-T\u00f3th-K", "name": { "family": "Fejes-T\u00f3th", "given": "Katalin" }, "orcid": "0000-0001-6558-2636" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A somatic piRNA pathway regulates epithelial-to-mesenchymal transition of chick neural crest cells", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nThis version posted April 30, 2021. \n\nWe thank members of the Bronner, Fejes Toth and Aravin labs for helpful discussions. We thank Maria Ninova for advice on RNA-seq analysis, as well as for making the pingpong script available for our use. We also thank Michael Piacentino for advice with imaging analysis and providing FIJI macros for our use. We thank Qing Tang for providing us with the mini CMV promoter. We acknowledge the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for library prep and sequencing of our CRISPR RNA-seq experiment, and in particular thank Igor Antoshechkin for advice on data analysis, as well as ensuring that our small RNA libraries got sequenced during the COVID-19 pandemic. This work is supported by NIH grants R01GM110217 to KFT and R35NS111564 to MB. RG was supported by the NSF GRFP fellowship. \n\nAuthor contributions: RG, MB and KFT designed experiments; RG conducted all experiments and data analysis; RG, MB and KFT wrote the manuscript. \n\nThe authors declare no competing financial interests. \n\nData availability statement: All raw sequencing data generated for this publication is available upon reasonable request from the corresponding authors, and will available through Gene Expression Omnibus upon publication. Previously published specified neural crest datasets are available from NCBI BioProject# PRJNA497902.\n\nSubmitted - 2021.04.30.442165v1.full.pdf
", "abstract": "In the metazoan germline, Piwi proteins play an essential regulatory role in maintenance of stemness and self-renewal by piRNA-mediated repression of transposable elements. To date, the activity of Piwi proteins and the piRNA pathway in vertebrates was believed to be confined to the gonads. Our results reveal expression of Piwil1 in a vertebrate somatic cell type, the neural crest \u2014 a migratory embryonic stem cell population. We show that Piwil1 is expressed at low levels throughout chick neural crest development, peaking just before neural crest cells undergo an epithelial-to-mesenchymal transition to leave the neural tube and migrate into the periphery. Importantly, loss of Piwil1 impedes neural crest emigration. Small RNA sequencing reveals somatic piRNAs with sequence signatures of an active ping pong loop. Coupled with Piwil1 knockout RNA-seq, our data suggest that Piwil1 regulates expression of the transposon derived gene ERNI in the chick dorsal neural tube, which in turn suppresses Sox2 expression to precisely control the timing of neural crest specification and emigration. Our work provides mechanistic insight into a novel function of the piRNA pathway as a regulator of somatic development in vertebrates.", "date": "2021-05-05", "date_type": "published", "id_number": "CaltechAUTHORS:20210504-121027939", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210504-121027939", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01GM110217" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NSF Graduate Research Fellowship" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2021.04.30.442165", "primary_object": { "basename": "2021.04.30.442165v1.full.pdf", "url": "https://authors.library.caltech.edu/records/zx2p9-8d270/files/2021.04.30.442165v1.full.pdf" }, "resource_type": "monograph", "pub_year": "2021", "author_list": "Galton, Riley; Fejes-T\u00f3th, Katalin; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nhq4t-c4d54", "eprint_id": 108401, "eprint_status": "archive", "datestamp": "2023-08-20 02:31:29", "lastmod": "2023-12-22 23:13:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Christensen-Jens-B", "name": { "family": "Christensen", "given": "Jens B." }, "orcid": "0000-0003-4617-3866" }, { "id": "Urrutia-Hugo-A", "name": { "family": "Urrutia", "given": "Hugo A." }, "orcid": "0000-0002-2970-6918" }, { "id": "Vieceli-Felipe-M", "name": { "family": "Vieceli", "given": "Felipe M." }, "orcid": "0000-0001-5142-8224" }, { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "CRISPR-Cas9, Chick embryology, Clonal analysis, Retroviruses, Migration, Genome editing, Neural crest", "note": "\u00a9 2021. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. \n\nReceived 3 June 2020; Accepted 23 February 2021. \n\nFor technical assistance, we thank Andres Collazo, Steven Wilbert and Giada Spigolon with the Caltech Biological Imaging facility of the Beckman Institute. We thank members of the Bronner lab for helpful discussions. \n\nAuthor contributions: Conceptualization: S.G., M.E.B.; Methodology: S.G., Y.L.; Validation: S.G.; Formal analysis: S.G.; Investigation: S.G., Y.L., W.T., J.B.C., H.A.U., F.M.V., M.L.P., M.E.B.; Resources: Y.L.; Writing - original draft: S.G., Y.L., W.T., H.A.U., M.E.B.; Writing - review & editing: S.G., Y.L., M.L.P., M.E.B.; Visualization: S.G., W.T., M.L.P.; Supervision: S.G., M.E.B.; Funding acquisition: M.E.B. \n\nThis work is supported by the National Institutes of Health (R01DE027568 to M.E.B. and K99DE029240 to M.L.P.), the American Heart Association (predoctoral fellowship 18PRE34050063 to S.G.) and the Otto Brunns Fund 82318917 to J.B.C. Open access funding provided by the California Institute of Technology (CALTECH). Deposited in PMC for immediate release. \n\nThe authors declare no competing or financial interests.\n\nPublished - dev193565.pdf
Supplemental Material - dev193565supp.pdf
", "abstract": "An important strategy for establishing mechanisms of gene function during development is through mutation of individual genes and analysis of subsequent effects on cell behavior. Here, we present a single-plasmid approach for genome editing in chick embryos to study experimentally perturbed cells in an otherwise normal embryonic environment. To achieve this, we have engineered a plasmid that encodes Cas9 protein, gene-specific guide RNA (gRNA), and a fluorescent marker within the same construct. Using transfection- and electroporation-based approaches, we show that this construct can be used to perturb gene function in early embryos as well as human cell lines. Importantly, insertion of this cistronic construct into replication-incompetent avian retroviruses allowed us to couple gene knockouts with long-term lineage analysis. We demonstrate the application of our newly engineered constructs and viruses by perturbing \u03b2-catenin in vitro and Sox10, Pax6 and Pax7 in the neural crest, retina, and neural tube and segmental plate in vivo, respectively. Together, this approach enables genes of interest to be knocked out in identifiable cells in living embryos and can be broadly applied to numerous genes in different embryonic tissues.", "date": "2021-04", "date_type": "published", "publication": "Development", "volume": "148", "number": "7", "publisher": "Company of Biologists", "pagerange": "Art. No. dev193565", "id_number": "CaltechAUTHORS:20210311-131531358", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210311-131531358", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "American Heart Association", "grant_number": "18PRE34050063" }, { "agency": "Otto Brunns Fund", "grant_number": "82318917" }, { "agency": "Caltech" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1242/dev.193565", "pmcid": "PMC8077534", "primary_object": { "basename": "dev193565.pdf", "url": "https://authors.library.caltech.edu/records/nhq4t-c4d54/files/dev193565.pdf" }, "related_objects": [ { "basename": "dev193565supp.pdf", "url": "https://authors.library.caltech.edu/records/nhq4t-c4d54/files/dev193565supp.pdf" } ], "resource_type": "article", "pub_year": "2021", "author_list": "Gandhi, Shashank; Li, Yuwei; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5xh1a-avc73", "eprint_id": 108551, "eprint_status": "archive", "datestamp": "2023-08-22 09:19:58", "lastmod": "2023-12-22 23:13:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Li-Ang", "name": { "family": "Li", "given": "Ang" }, "orcid": "0000-0002-8784-4702" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Clonal analysis and dynamic imaging identify multipotency of individual Gallus gallus caudal hindbrain neural crest cells toward cardiac and enteric fates", "ispublished": "pub", "full_text_status": "public", "keywords": "Cell biology; Developmental biology; Stem cells", "note": "\u00a9 The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nReceived 11 March 2020; Accepted 22 February 2021; Published 25 March 2021. \n\nWe thank Dr. Carlos Lois and the Biological Imaging Facility of the Beckman Institute for sharing equipment. This work is supported by NIHRO1HL14058 to M.E.B. \n\nData availability: The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files or from the corresponding author upon reasonable request. \n\nAuthor Contributions: W.T. and M.E.B. conceived the project. W.T. and Y.L designed the experiments. W.T. performed the experiments. Y.L. established single-cell photoconversion and provided help with imaging analysis. A.L. performed quantitative analysis. W.T., Y.L., and M. E.B wrote the manuscript with consultation from A.L. \n\nThe authors declare no competing interests. \n\nPeer review information: Nature Communications thanks David McCauley and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.\n\nPublished - s41467-021-22146-8.pdf
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Supplemental Material - 41467_2021_22146_MOESM3_ESM.mp4
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Supplemental Material - 41467_2021_22146_MOESM8_ESM.xlsx
Supplemental Material - 41467_2021_22146_MOESM9_ESM.pdf
", "abstract": "Neural crest stem cells arising from caudal hindbrain (often called cardiac and posterior vagal neural crest) migrate long distances to form cell types as diverse as heart muscle and enteric ganglia, abnormalities of which lead to common congenital birth defects. Here, we explore whether individual caudal hindbrain neural crest precursors are multipotent or predetermined toward these particular fates and destinations. To this end, we perform lineage tracing of chick neural crest cells at single-cell resolution using two complementary approaches: retrovirally mediated multiplex clonal analysis and single-cell photoconversion. Both methods show that the majority of these neural crest precursors are multipotent with many clones producing mesenchymal as well as neuronal derivatives. Time-lapse imaging demonstrates that sister cells can migrate in distinct directions, suggesting stochasticity in choice of migration path. Perturbation experiments further identify guidance cues acting on cells in the pharyngeal junction that can influence this choice; loss of CXCR4 signaling results in failure to migrate to the heart but no influence on migration toward the foregut, whereas loss of RET signaling does the opposite. Taken together, the results suggest that environmental influences rather than intrinsic information govern cell fate choice of multipotent caudal hindbrain neural crest cells.", "date": "2021-03-25", "date_type": "published", "publication": "Nature Communications", "volume": "12", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 1894", "id_number": "CaltechAUTHORS:20210325-075837590", "issn": "2041-1723", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210325-075837590", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1HL14058" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1038/s41467-021-22146-8", "pmcid": "PMC7994390", "primary_object": { "basename": "41467_2021_22146_MOESM2_ESM.pdf", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM2_ESM.pdf" }, "related_objects": [ { "basename": "41467_2021_22146_MOESM4_ESM.mp4", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM4_ESM.mp4" }, { "basename": "41467_2021_22146_MOESM5_ESM.mp4", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM5_ESM.mp4" }, { "basename": "41467_2021_22146_MOESM7_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM7_ESM.xlsx" }, { "basename": "41467_2021_22146_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM1_ESM.pdf" }, { "basename": "41467_2021_22146_MOESM3_ESM.mp4", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM3_ESM.mp4" }, { "basename": "41467_2021_22146_MOESM6_ESM.mp4", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM6_ESM.mp4" }, { "basename": "41467_2021_22146_MOESM8_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM8_ESM.xlsx" }, { "basename": "41467_2021_22146_MOESM9_ESM.pdf", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM9_ESM.pdf" }, { "basename": "s41467-021-22146-8.pdf", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/s41467-021-22146-8.pdf" }, { "basename": "41467_2021_22146_MOESM10_ESM.pdf", "url": "https://authors.library.caltech.edu/records/5xh1a-avc73/files/41467_2021_22146_MOESM10_ESM.pdf" } ], "resource_type": "article", "pub_year": "2021", "author_list": "Tang, Weiyi; Li, Yuwei; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2pdhw-d0q05", "eprint_id": 108156, "eprint_status": "archive", "datestamp": "2023-08-22 08:51:06", "lastmod": "2024-01-15 21:22:39", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "\u0160tundl-Jan", "name": { "family": "Stundl", "given": "Jan" }, "orcid": "0000-0002-3740-3378" }, { "id": "Bertucci-Paola-Y", "name": { "family": "Bertucci", "given": "Paola Y." }, "orcid": "0000-0002-4116-7689" }, { "id": "Lauri-Antonella", "name": { "family": "Lauri", "given": "Antonella" }, "orcid": "0000-0003-2260-182X" }, { "id": "Arendt-Detlev", "name": { "family": "Arendt", "given": "Detlev" }, "orcid": "0000-0001-7833-050X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolution of new cell types at the lateral neural border", "ispublished": "unpub", "full_text_status": "restricted", "keywords": "Neural crest; Cranial placodes; Evolution; Lateral neural border; Bilaterians; Chordates; Peripheral nervous system; Homology", "note": "\u00a9 2021 Elsevier Inc. \n\nAvailable online 6 February 2021.", "abstract": "During the course of evolution, animals have become increasingly complex by the addition of novel cell types and regulatory mechanisms. A prime example is represented by the lateral neural border, known as the neural plate border in vertebrates, a region of the developing ectoderm where presumptive neural and non-neural tissue meet. This region has been intensively studied as the source of two important embryonic cell types unique to vertebrates\u2014the neural crest and the ectodermal placodes\u2014which contribute to diverse differentiated cell types including the peripheral nervous system, pigment cells, bone, and cartilage. How did these multipotent progenitors originate in animal evolution? What triggered the elaboration of the border during the course of chordate evolution? How is the lateral neural border patterned in various bilaterians and what is its fate? Here, we review and compare the development and fate of the lateral neural border in vertebrates and invertebrates and we speculate about its evolutionary origin. Taken together, the data suggest that the lateral neural border existed in bilaterian ancestors prior to the origin of vertebrates and became a developmental source of exquisite evolutionary change that frequently enabled the acquisition of new cell types.", "date": "2021-02-06", "date_type": "published", "publisher": "Elsevier", "place_of_pub": "Cambridge, MA", "pagerange": "173-205", "id_number": "CaltechAUTHORS:20210223-141057698", "isbn": "9780128149683", "book_title": "Evolutionary Developmental Biology", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210223-141057698", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "contributors": { "items": [ { "id": "Gilbert-Scott-F", "name": { "family": "Gilbert", "given": "Scott F." } } ] }, "doi": "10.1016/bs.ctdb.2020.11.005", "resource_type": "book_section", "pub_year": "2021", "author_list": "Stundl, Jan; Bertucci, Paola Y.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4x7aj-dff34", "eprint_id": 108274, "eprint_status": "archive", "datestamp": "2023-08-20 01:46:53", "lastmod": "2023-12-22 23:41:27", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Transcriptomic Identification of Draxin-Responsive Targets During Cranial Neural Crest EMT", "ispublished": "pub", "full_text_status": "public", "keywords": "Draxin, Wnt, neural crest, EMT, craniofacial development", "note": "\u00a9 2021 Hutchins, Piacentino and Bronner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. \n\nReceived: 30 October 2020; Accepted: 07 January 2021;\nPublished: 03 February 2021. \n\nWe thank A. Collazo and G. Spigolon for imaging assistance at the Caltech Biological Imaging Facility; P. Cannon and R. Diamond of the Caltech Flow Cytometry Cell Sorting Facility for cell sorting assistance; I. Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for sequencing of our RNA-seq libraries; and M. Martik and S. Gandhi for assistance with data processing. \n\nThis work was supported by the National Institutes of Health (R01DE027538 and R01DE027568 to MB, K99DE028592 to EH, and K99DE029240 to MP).\n\nAuthor Contributions:\nEH, MP, and MB conceived the project and conducted the experimental design and data interpretation. EH and MP performed the cell dissociations, library preparations, and RNA-seq analyses. EH performed the functional annotation, hybridization chain reaction experiments, imaging, quantitation, and statistical analyses. EH and MB wrote the manuscript with editing by MP. All authors contributed to the article and approved the submitted version.\n\nThe authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. \n\nEthics Statement: Ethical review and approval was not required for the animal study because our study uses chicken embryos at E1-2. These are not considered vertebrate embryos until E10, and thus we do not require ethics committee approvals or protocols, as they are not considered vertebrates at the stages we work.\n\nPublished - fphys-12-624037.pdf
Supplemental Material - Image_1_Transcriptomic_Identification_of_Draxin-Responsive_Targets_During_Cranial_Neural_Crest_EMT.TIF
", "abstract": "Canonical Wnt signaling plays an essential role in proper craniofacial morphogenesis, at least partially due to regulation of various aspects of cranial neural crest development. In an effort to gain insight into the etiology of craniofacial abnormalities resulting from Wnt signaling and/or cranial neural crest dysfunction, we sought to identify Wnt-responsive targets during chick cranial neural crest development. To this end, we leveraged overexpression of a canonical Wnt antagonist, Draxin, in conjunction with RNA-sequencing of cranial neural crest cells that have just activated their epithelial\u2013mesenchymal transition (EMT) program. Through differential expression analysis, gene list functional annotation, hybridization chain reaction (HCR), and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we validated a novel downstream target of canonical Wnt signaling in cranial neural crest \u2013 RHOB \u2013 and identified possible signaling pathway crosstalk underlying cranial neural crest migration. The results reveal novel putative targets of canonical Wnt signaling during cranial neural crest EMT and highlight important intersections across signaling pathways involved in craniofacial development.", "date": "2021-02-03", "date_type": "published", "publication": "Frontiers in Physiology", "volume": "12", "publisher": "Frontiers Research Foundation", "pagerange": "Art. No. 624037", "id_number": "CaltechAUTHORS:20210302-141852646", "issn": "1664-042X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20210302-141852646", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "K99DE029240" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.3389/fphys.2021.624037", "pmcid": "PMC7886793", "primary_object": { "basename": "Image_1_Transcriptomic_Identification_of_Draxin-Responsive_Targets_During_Cranial_Neural_Crest_EMT.TIF", "url": "https://authors.library.caltech.edu/records/4x7aj-dff34/files/Image_1_Transcriptomic_Identification_of_Draxin-Responsive_Targets_During_Cranial_Neural_Crest_EMT.TIF" }, "related_objects": [ { "basename": "fphys-12-624037.pdf", "url": "https://authors.library.caltech.edu/records/4x7aj-dff34/files/fphys-12-624037.pdf" } ], "resource_type": "article", "pub_year": "2021", "author_list": "Hutchins, Erica J.; Piacentino, Michael L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rw28y-j1w02", "eprint_id": 105484, "eprint_status": "archive", "datestamp": "2023-08-20 01:38:53", "lastmod": "2023-12-22 23:23:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Niklasson-Camilla-U", "name": { "family": "Niklasson", "given": "Camilla U." } }, { "id": "Fredlund-Elina", "name": { "family": "Fredlund", "given": "Elina" } }, { "id": "Monni-Emanuela", "name": { "family": "Monni", "given": "Emanuela" }, "orcid": "0000-0003-0083-1050" }, { "id": "Lindvall-Jessica-M", "name": { "family": "Lindvall", "given": "Jessica M." }, "orcid": "0000-0002-5042-8481" }, { "id": "Kokaia-Zaal", "name": { "family": "Kokaia", "given": "Zaal" }, "orcid": "0000-0003-2296-2449" }, { "id": "Hammarlund-Emma-U", "name": { "family": "Hammarlund", "given": "Emma U." }, "orcid": "0000-0001-7625-4793" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Mohlin-Sofie", "name": { "family": "Mohlin", "given": "Sofie" }, "orcid": "0000-0002-2458-3963" } ] }, "title": "Hypoxia inducible factor\u20102\u03b1 importance for migration, proliferation, and self\u2010renewal of trunk neural crest cells", "ispublished": "pub", "full_text_status": "public", "keywords": "embryogenesis, HIF-2\u03b1, migration, neural crest, stem cells, trunk neural crest", "note": "\u00a9 2020 The Authors. Developmental Dynamics published by Wiley Periodicals LLC on behalf of American Association of Anatomists. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. \n\nIssue Online: 01 February 2021; Version of Record online: 26 September 2020; Accepted manuscript online: 17 September 2020; Manuscript accepted: 11 September 2020; Manuscript revised: 02 September 2020; Manuscript received: 29 July 2020. \n\nThe authors would like to thank Erica Hutchins, Shashank Gandhi, and Siv Beckman for skillful technical assistance, Felipe Vieceli for providing templates for in situ hybridization probes and Anni Glud and Ronnie N. Glud for providing microsensor technique and expertise. The authors thank Center for Translational Genomics, Lund University, and Clinical Genomics Lund, SciLifeLab for providing sequencing service. Support by NBIS (National Bioinformatics Infrastructure Sweden) is gratefully acknowledged. This work was supported by the Swedish Cancer Society, the Swedish Childhood Cancer Fund, the Crafoord Foundation, Jeansson Foundations, Ollie and Elof Ericsson's Foundation, the Mary Bev\u00e9 Foundation, Magnus Bergvall's Foundation, the Thelma Zo\u00e9ga Foundation for medical research, Hans von Kantzow's Foundation, the Royal Physiographic Society of Lund, the Gyllenstierna Krapperup's Foundation, and Gunnar Nilssons Cancerstiftelse (to S. M.), DE027568 and R01HL14058 (to M. E. B.). \n\nThe authors declare no conflict of interests. \n\nAuthor Contributions: Camilla U. Niklasson: Investigation, writing\u2010review and editing. Elina Fredlund: Investigation, writing\u2010review and editing. Emanuela Monni: Resources, writing\u2010review and editing. Jessica M. Lindvall: Data curation, formal analysis, software, writing\u2010review and editing. Zaal Kokaia: Resources, writing\u2010review and editing. Emma U. Hammarlund: Formal analysis, investigation, methodology, validation, visualization, writing\u2010review and editing. Marianne E. Bronner: Conceptualization, funding acquisition, methodology, supervision, writing\u2010review and editing. Sofie Mohlin: Conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, supervision, validation, visualization, writing\u2010original draft, writing\u2010review and editing.\n\nPublished - dvdy.253.pdf
", "abstract": "Background: The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)\u20102\u03b1 is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development. \n\nResults: Here, we show that HIF\u20102\u03b1 is expressed in trunk neural crest cells of human, murine, and avian embryos. Knockdown as well as overexpression of HIF\u20102\u03b1 in vivo causes developmental delays, induces proliferation, and self\u2010renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF\u20102\u03b1 reveal enrichment of genes associated with cancer, invasion, epithelial\u2010to\u2010mesenchymal transition, and growth arrest. \n\nConclusions: Taken together, these results suggest that expression levels of HIF\u20102\u03b1 must be strictly controlled during normal trunk neural crest development and that dysregulated levels affects several important features connected to stemness, migration, and development.", "date": "2021-02", "date_type": "published", "publication": "Developmental Dynamics", "volume": "250", "number": "2", "publisher": "Wiley", "pagerange": "191-236", "id_number": "CaltechAUTHORS:20200923-074659440", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200923-074659440", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Bioinformatics Infrastructure Sweden" }, { "agency": "Swedish Cancer Society" }, { "agency": "Swedish Childhood Cancer Fund" }, { "agency": "Crafoord Foundation" }, { "agency": "Jeansson Foundations" }, { "agency": "Ollie and Elof Ericsson's Foundation" }, { "agency": "Mary Bev\u00e9 Foundation" }, { "agency": "Magnus Bergvall's Foundation" }, { "agency": "Thelma Zo\u00e9ga Foundation" }, { "agency": "Hans von Kantzow's Foundation" }, { "agency": "Royal Physiographic Society of Lund" }, { "agency": "Gyllenstierna Krapperup's Foundation" }, { "agency": "Gunnar Nilssons Cancerstiftelse" }, { "agency": "NIH", "grant_number": "DE027568" }, { "agency": "NIH", "grant_number": "R01HL14058" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1002/dvdy.253", "primary_object": { "basename": "dvdy.253.pdf", "url": "https://authors.library.caltech.edu/records/rw28y-j1w02/files/dvdy.253.pdf" }, "resource_type": "article", "pub_year": "2021", "author_list": "Niklasson, Camilla U.; Fredlund, Elina; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j4xaj-z3317", "eprint_id": 106204, "eprint_status": "archive", "datestamp": "2023-08-20 00:15:11", "lastmod": "2023-12-22 23:08:41", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Gonzalez-Walter-G", "name": { "family": "Gonzalez", "given": "Walter G." }, "orcid": "0000-0003-1310-9323" }, { "id": "Andreev-Andrey", "name": { "family": "Andreev", "given": "Andrey" }, "orcid": "0000-0002-7833-1390" }, { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Cunha-Alexandre", "name": { "family": "Cunha", "given": "Alexandre" }, "orcid": "0000-0002-2541-6024" }, { "id": "Prober-D-A", "name": { "family": "Prober", "given": "David" }, "orcid": "0000-0002-7371-4675" }, { "id": "Lois-C", "name": { "family": "Lois", "given": "Carlos" }, "orcid": "0000-0002-7305-2317" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Macropinocytosis-mediated membrane recycling drives neural crest migration by delivering F-actin to the lamellipodium", "ispublished": "pub", "full_text_status": "public", "keywords": "membrane recycling | actin turnover | macropinocytosis | cell migration | neural crests", "note": "\u00a9 2020 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). \n\nContributed by Marianne E. Bronner, September 22, 2020 (sent for review June 8, 2020; reviewed by Angela Nieto and Tatjana Piotrowski) \n\nWe thank Pierre Martineau for sharing reagents and Beckman Institute Biological Imaging Facility at Caltech for sharing equipment. We thank the Beckman Institute at Caltech for financial support to the Center for Advanced Methods in Biological Image Analysis (A.C.). W.G.G. is supported by the Della Martin Foundation, the American Heart Association, and the Burroughs Wellcome Fund. S.G. is supported by the American Heart Association. This project is supported by DE024157 and R35NS111564 (to M.E.B.). \n\nData Availability. All study data are included in the article and supporting information. \n\nAuthor contributions: Y.L. and W.G.G. designed research; Y.L., W.T., and S.G. performed research; Y.L., W.G.G., and A.A. contributed new reagents/analytic tools; Y.L., W.G.G., A.A., A.C., D.P., and C.L. analyzed data; and Y.L., W.G.G., and M.E.B. wrote the paper. \n\nReviewers: A.N., Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Cient\u00edficas\u2013Universidad Miguel Hern\u00e1ndez; and T.P., Stowers Institute for Medical Research. \n\nCompeting interest statement: M.E.B. and A.N. are listed as coauthors on a 2020 Consensus Statement. They did not collaborate directly on the paper. \n\nThis article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.2007229117/-/DCSupplemental.\n\nPublished - 27400.full.pdf
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Supplemental Material - pnas.2007229117.sm18.avi
", "abstract": "Individual cell migration requires front-to-back polarity manifested by lamellipodial extension. At present, it remains debated whether and how membrane motility mediates this cell morphological change. To gain insights into these processes, we perform live imaging and molecular perturbation of migrating chick neural crest cells in vivo. Our results reveal an endocytic loop formed by circular membrane flow and anterograde movement of lipid vesicles, resulting in cell polarization and locomotion. Rather than clathrin-mediated endocytosis, macropinosomes encapsulate F-actin in the cell body, forming vesicles that translocate via microtubules to deliver actin to the anterior. In addition to previously proposed local conversion of actin monomers to polymers, we demonstrate a surprising role for shuttling of F-actin across cells for lamellipodial expansion. Thus, the membrane and cytoskeleton act in concert in distinct subcellular compartments to drive forward cell migration.", "date": "2020-11-03", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "117", "number": "44", "publisher": "National Academy of Sciences", "pagerange": "27400-27411", "id_number": "CaltechAUTHORS:20201022-090820908", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20201022-090820908", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Caltech Beckman Institute" }, { "agency": "Della Martin Foundation" }, { "agency": "American Heart Association" }, { "agency": "Burroughs Wellcome Fund" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "R35NS111564" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1073/pnas.2007229117", "pmcid": "PMC7959501", "primary_object": { "basename": "pnas.2007229117.sm13.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm13.avi" }, "related_objects": [ { "basename": "27400.full.pdf", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/27400.full.pdf" }, { "basename": "pnas.2007229117.sm08.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm08.avi" }, { "basename": "pnas.2007229117.sm10.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm10.avi" }, { "basename": "pnas.2007229117.sm11.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm11.avi" }, { "basename": "pnas.2007229117.sm15.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm15.avi" }, { "basename": "pnas.2007229117.sm17.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm17.avi" }, { "basename": "pnas.2007229117.sapp.pdf", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sapp.pdf" }, { "basename": "pnas.2007229117.sm01.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm01.avi" }, { "basename": "pnas.2007229117.sm02.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm02.avi" }, { "basename": "pnas.2007229117.sm03.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm03.avi" }, { "basename": "pnas.2007229117.sm04.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm04.avi" }, { "basename": "pnas.2007229117.sm06.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm06.avi" }, { "basename": "pnas.2007229117.sm05.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm05.avi" }, { "basename": "pnas.2007229117.sm07.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm07.avi" }, { "basename": "pnas.2007229117.sm09.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm09.avi" }, { "basename": "pnas.2007229117.sm12.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm12.avi" }, { "basename": "pnas.2007229117.sm14.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm14.avi" }, { "basename": "pnas.2007229117.sm16.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm16.avi" }, { "basename": "pnas.2007229117.sm18.avi", "url": "https://authors.library.caltech.edu/records/j4xaj-z3317/files/pnas.2007229117.sm18.avi" } ], "resource_type": "article", "pub_year": "2020", "author_list": "Li, Yuwei; Gonzalez, Walter G.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qpjcw-eyp10", "eprint_id": 105180, "eprint_status": "archive", "datestamp": "2023-08-22 07:10:24", "lastmod": "2023-12-22 23:23:54", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stundl-J", "name": { "family": "Stundl", "given": "Jan" } }, { "id": "Pospisilova-A", "name": { "family": "Pospisilova", "given": "Anna" } }, { "id": "Mat\u011bjkov\u00e1-T", "name": { "family": "Mat\u011bjkov\u00e1", "given": "Tereza" } }, { "id": "Psenicka-M", "name": { "family": "Psenicka", "given": "Martin" }, "orcid": "0000-0002-3808-7856" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Cerny-R", "name": { "family": "Cerny", "given": "Robert" } } ] }, "title": "Migratory patterns and evolutionary plasticity of cranial neural crest cells in ray-finned fishes", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Vertebrates; Craniofacial; Evolution; Neurulation", "note": "\u00a9 2020 Elsevier Inc. \n\nReceived 27 April 2020, Revised 13 August 2020, Accepted 14 August 2020, Available online 21 August 2020. \n\nWe thank Vojt\u011bch Miller and Karel Kodej\u0161 for the bichir colony care and logistic support; Roman Fran\u011bk, Michaela Fu\u010d\u00edkov\u00e1, David Gela, Martin Kahanec and Marek Rodina for the sterlet spawns; Lenin Arias-Rodriguez and Adriana Osorio-P\u00e9rez for the gar spawns; Radek Holcman for the northern pike spawns; Peter Fabian (ISH), and Brian D. Metscher (microCT) for technical assistance; Martin Mina\u0159\u00edk for the pharyngeal endoderm reconstructions of the sterlet David Jandzik for the generous gift of primer sequences of Hand2_Ps; Vladimir Soukup and Jana \u0160tundlov\u00e1 for critical reading of the manuscript. Special thanks are due to Radek \u0160anda for the support and continuous interest in our work. This study was supported by the European Union's Horizon 2020 research and innovation program under the Marie Sk\u0142odowska-Curie grant agreement No. 897949 (to JS), the Charles University grant GAUK 1448514 (to JS), NIH R35NS111564 (to MEB), the Czech Science Foundation GACR 19-18634S (to RC), and the Ministry of Education, Youth and Sports of the Czech Republic\u2014project CENAKVA LM2018099 and Biodiversity CZ.02.1.01/0.0/0.0/16_025/0007370) (to MP).\n\nSupplemental Material - 1-s2.0-S0012160620302281-mmc1.pdf
", "abstract": "The cranial neural crest (CNC) arises within the developing central nervous system, but then migrates away from the neural tube in three consecutive streams termed mandibular, hyoid and branchial, respectively, according to the order along the anteroposterior axis. While the process of neural crest emigration generally follows a conserved anterior to posterior sequence across vertebrates, we find that ray-finned fishes (bichir, sterlet, gar, and pike) exhibit several heterochronies in the timing and order of CNC emergence that influences their subsequent migratory patterns. First, emigration of the cranial neural crest in these fishes occurs prematurely compared to other vertebrates, already initiating during early neurulation and well before neural tube closure. Second, delamination of the hyoid stream occurs prior to the more anterior mandibular stream; this is associated with early morphogenesis of key hyoid structures like external gills (bichir), a large opercular flap (gar) or first forming cartilage (pike). In sterlet, the hyoid and branchial CNC cells form a single hyobranchial sheet, which later segregates in concert with second pharyngeal pouch morphogenesis. Taken together, the results show that despite generally conserved migratory patterns, heterochronic alterations in the timing of emigration and pattern of migration of CNC cells accompanies morphological diversity of ray-finned fishes.", "date": "2020-11-01", "date_type": "published", "publication": "Developmental Biology", "volume": "467", "number": "1-2", "publisher": "Elsevier", "pagerange": "14-29", "id_number": "CaltechAUTHORS:20200831-143448217", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200831-143448217", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Marie Curie Fellowship", "grant_number": "897949" }, { "agency": "Charles University", "grant_number": "1448514" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "Czech Science Foundation", "grant_number": "19-18634S" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "CENAKVA LM2018099" }, { "agency": "Ministry of Education, Youth and Sports (Czech Republic)", "grant_number": "CZ.02.1.01/0.0/0.0/16_025/0007370" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.ydbio.2020.08.007", "primary_object": { "basename": "1-s2.0-S0012160620302281-mmc1.pdf", "url": "https://authors.library.caltech.edu/records/qpjcw-eyp10/files/1-s2.0-S0012160620302281-mmc1.pdf" }, "resource_type": "article", "pub_year": "2020", "author_list": "Stundl, Jan; Pospisilova, Anna; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6cgjb-sa090", "eprint_id": 106175, "eprint_status": "archive", "datestamp": "2023-08-19 23:55:23", "lastmod": "2023-12-22 23:38:41", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Andrews-Cecelia-J", "name": { "family": "Andrews", "given": "Cecelia J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nPosted October 19, 2020. \n\nWe would like to thank Megan Martik, Erdin\u00e7 Sezgin, Justin Bois, and Steven Wilbert for valuable discussion on experiment design and analysis, and Alexis Camacho-Avila and Gabriel da Silva Pescador for technical support. We thank Catherine Berlot, Anna-Katerina Hadjantonakis, Randall Moon, and Elisa Mart\u00ed for sharing reagents. Confocal imaging was supported by the Caltech Beckman Institute and the Arnold and Mabel Beckman Foundation to the Biological Imaging Facility. Funding for this work comes from the National Institutes of Health grants K99DE029240 to M.L.P., K99DE028592 to E.J.H, R01DE027538 and R01DE027568 to M.E.B, and from the Caltech Summer Undergraduate Research Fellowship (SURF) to C.J.A. \n\nAuthor Contributions: Conceptualization: M.L.P. and M.E.B.\nExperiment design: M.L.P., E.J.H., and M.E.B. Experimentation: M.L.P., E.J.H., and C.J.A. Data analysis: M.L.P. and C.J.A. Data interpretation: M.L.P., E.J.H., and M.E.B. Manuscript preparation: M.L.P. and M.E.B. Manuscript editing: E.J.H. \n\nThe authors declare no competing interests. \n\nData Availability: All data that support the findings of this study are available from the corresponding author upon reasonable request. The Smpd3 mRNA sequence has been submitted to GenBank (Accession # Pending). \n\nCode Availability: All data analysis code used in this study are available from the corresponding author upon reasonable request.\n\nSubmitted - 2020.10.18.344523v2.full.pdf
", "abstract": "Epithelial-to-mesenchymal transition (EMT) is a dramatic change in cellular physiology during development and metastasis which involves coordination between cell signaling, adhesion, and membrane protrusions. These processes all involve dynamic changes in the plasma membrane, yet how membrane lipid content regulates membrane function during developmental EMT remains incompletely understood. By screening for differential expression of lipid-modifying genes over the course of EMT in avian neural crest, we have identified the ceramide-producing enzyme neutral sphingomyelinase 2 (nSMase2) as a critical regulator of a developmental EMT. nSMase2 expression begins at the onset of EMT, and in vivo knockdown experiments demonstrate that nSMase2 is necessary for neural crest migration. Further, we find that nSMase2 promotes Wnt and BMP signaling, and is required to activate the mesenchymal gene expression program. Mechanistically, we show that nSMase2 is sufficient to induce endocytosis, and that inhibition of endocytosis mimics nSMase2 knockdown. Our results support a model in which nSMase2 is expressed at the onset of neural crest EMT to produce ceramide and induce membrane curvature, thus increasing endocytosis of Wnt and BMP signaling complexes and activating pro-migratory gene expression. These results highlight the critical role of plasma membrane lipid metabolism in regulating transcriptional changes during developmental EMT programs.", "date": "2020-10-21", "date_type": "published", "id_number": "CaltechAUTHORS:20201021-071531181", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20201021-071531181", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Caltech Beckman Institute" }, { "agency": "Arnold and Mabel Beckman Foundation" }, { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" } ] }, "local_group": { "items": [ { "id": "Tianqiao-and-Chrissy-Chen-Institute-for-Neuroscience" }, { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2020.10.18.344523", "primary_object": { "basename": "2020.10.18.344523v2.full.pdf", "url": "https://authors.library.caltech.edu/records/6cgjb-sa090/files/2020.10.18.344523v2.full.pdf" }, "resource_type": "monograph", "pub_year": "2020", "author_list": "Piacentino, Michael L.; Hutchins, Erica J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zgjqc-qe047", "eprint_id": 106093, "eprint_status": "archive", "datestamp": "2023-08-19 23:53:08", "lastmod": "2023-12-22 23:14:05", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Chacon-Jose", "name": { "family": "Chacon", "given": "Jose" }, "orcid": "0000-0001-7965-3976" }, { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license. \n\nVersion 1 - October 14. 2020; Version 2 - July 27, 2022. \n\nWe thank A. Collazo and G. Spigolon for imaging assistance at the Caltech Biological Imaging Facility; M. Schwarzkopf and G. Shin (Molecular Technologies) for HCR probe design; I. Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for sequencing of RNA-seq libraries; S. Manohar for assistance with Axud1 3'-RACE; and G. da Silva Pescador and R. Galton for assistance with pilot experiments. \n\nThis work was supported by the National Institutes of Health [R01DE027538 and R01DE027568 to M.E.B; K99DE028592 to E.J.H; K99DE029240 to M.L.P.], the California State University\u2014Northridge BUILD PODER program [TL4GM118977], and the Amgen Foundation Caltech Amgen Scholars Program [J.C.]. \n\nThe authors declare no competing interests. \n\nData Availability: RNA-sequencing datasets have been deposited on NCBI under the accession number PRJNA861325. The 3' untranslated region (UTR) sequence for Axud1 has been deposited to GenBank under accession number ON920861.\n\nSubmitted - 2020.10.14.338715v2.full.pdf
", "abstract": "While neural crest development is known to be transcriptionally controlled via sequential activation of gene regulatory networks (GRNs), recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using available single cell RNA-sequencing datasets from avian embryos to identify potential post-transcriptional regulators, we found that Elavl1, which encodes for an RNA-binding protein with roles in transcript stability, was enriched in the premigratory cranial neural crest. Perturbation of Elavl1 resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN, phenotypes that are also observed with downregulation of the canonical Wnt inhibitor Draxin. That Draxin is the primary target for stabilization by Elavl1 during cranial neural crest specification was shown by RNA-sequencing, RNA-immunoprecipitation, RNA decay measurement and proximity ligation assays, further supporting the idea that the downregulation of neural crest specifier expression upon Elavl1 knockdown was largely due to loss of Draxin. Importantly, exogenous Draxin rescued cranial neural crest specification defects observed with Elavl1 knockdown. Thus, Elavl1 plays a critical a role in the maintenance of cranial neural crest specification via Draxin mRNA stabilization. Together, these data highlight an important intersection of post-transcriptional regulation with modulation of the neural crest specification GRN.", "date": "2020-10-16", "date_type": "published", "id_number": "CaltechAUTHORS:20201015-152733431", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20201015-152733431", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "California State University, Northridge" }, { "agency": "Amgen Scholars Program" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2020.10.14.338715", "primary_object": { "basename": "2020.10.14.338715v2.full.pdf", "url": "https://authors.library.caltech.edu/records/zgjqc-qe047/files/2020.10.14.338715v2.full.pdf" }, "resource_type": "monograph", "pub_year": "2020", "author_list": "Hutchins, Erica J.; Gandhi, Shashank; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2ygkx-wgq36", "eprint_id": 106289, "eprint_status": "archive", "datestamp": "2023-08-22 06:58:35", "lastmod": "2023-12-22 23:25:58", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest lineage analysis: from past to future trajectory", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Lineage tracing; Clonal analysis; Multipotency; Tumor development; Regeneration", "note": "\u00a9 2020 Published by The Company of Biologists Ltd. \n\nWe thank Dr Vicky Prince for helpful suggestions on the manuscript. \n\nThe authors declare no competing or financial interests. \n\nThe authors' research was funded by the National Institutes of Health (DE027568, R35NS111564 and NIHRO1HL14058 to M.E.B.). Deposited in PMC for release after 12 months.\n\nPublished - dev193193.full.pdf
", "abstract": "Since its discovery 150\u2005years ago, the neural crest has intrigued investigators owing to its remarkable developmental potential and extensive migratory ability. Cell lineage analysis has been an essential tool for exploring neural crest cell fate and migration routes. By marking progenitor cells, one can observe their subsequent locations and the cell types into which they differentiate. Here, we review major discoveries in neural crest lineage tracing from a historical perspective. We discuss how advancing technologies have refined lineage-tracing studies, and how clonal analysis can be applied to questions regarding multipotency. We also highlight how effective progenitor cell tracing, when combined with recently developed molecular and imaging tools, such as single-cell transcriptomics, single-molecule fluorescence in situ hybridization and high-resolution imaging, can extend the scope of neural crest lineage studies beyond development to regeneration and cancer initiation.", "date": "2020-10-15", "date_type": "published", "publication": "Development", "volume": "147", "number": "20", "publisher": "Company of Biologists", "pagerange": "Art. No. dev193193", "id_number": "CaltechAUTHORS:20201027-081451617", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20201027-081451617", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE027568" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NIH", "grant_number": "RO1HL14058" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1242/dev.193193", "pmcid": "PMC7595686", "primary_object": { "basename": "dev193193.full.pdf", "url": "https://authors.library.caltech.edu/records/2ygkx-wgq36/files/dev193193.full.pdf" }, "resource_type": "article", "pub_year": "2020", "author_list": "Tang, Weiyi and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7pgtc-7wf89", "eprint_id": 103799, "eprint_status": "archive", "datestamp": "2023-08-22 06:29:35", "lastmod": "2023-12-22 23:26:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Epithelial-to-mesenchymal transition and different migration strategies as viewed from the neural crest", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Developmental biology; Neural Crest; Epithelial-to-mesenchymal transition; Cell migration; Collective migration", "note": "\u00a9 2020 Elsevier Ltd. \n\nAvailable online 9 June 2020. \n\nThis review comes from a themed issue on Cell Dynamics; Edited by Diane Barber and Xavier Trepat. \n\nThe authors would like to acknowledge Dr. Erica Hutchins for valuable discussion. This work was supported by the National Institutes of Health (K99DE029240 to M.L.P., R01DE027538 and R01DE027568 to M.E.B.). The authors apologize to those authors they were unable to cite because of space limitations. \n\nConflict of interest statement: Nothing declared.", "abstract": "Epithelial-to-mesenchymal transition (EMT) is a dynamic process that produces migratory cells from epithelial precursors. However, EMT is not binary; rather it results in migratory cells which adopt diverse strategies including collective and individual cell migration to arrive at target destinations. Of the many embryonic cells that undergo EMT, the vertebrate neural crest is a particularly good example which has provided valuable insight into these processes. Neural crest cells from different species often adopt different migratory strategies with collective migration predominating in anamniotes, whereas individual cell migration is more prevalent in amniotes. Here, we will provide a perspective on recent work toward understanding the process of neural crest EMT focusing on how these cells undergo collective and individual cell migration.", "date": "2020-10", "date_type": "published", "publication": "Current Opinion in Cell Biology", "volume": "66", "publisher": "Elsevier", "pagerange": "43-50", "id_number": "CaltechAUTHORS:20200609-133046051", "issn": "0955-0674", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200609-133046051", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "K99DE029240" }, { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.ceb.2020.05.001", "resource_type": "article", "pub_year": "2020", "author_list": "Piacentino, Michael L.; Li, Yuwei; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wrtcf-y5749", "eprint_id": 105631, "eprint_status": "archive", "datestamp": "2023-08-19 23:28:01", "lastmod": "2023-12-22 23:09:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Maruszko-Krystyna", "name": { "family": "Maruszko", "given": "Krystyna" } }, { "id": "Park-Jong-H", "name": { "family": "Park", "given": "Jong H." } }, { "id": "Thomson-M-W", "name": { "family": "Thomson", "given": "Matthew" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Bimodal function of chromatin remodeler Hmga1 in neural crest induction and Wnt-dependent emigration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2020, Gandhi et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. \n\nReceived: 24 April 2020; Accepted: 23 September 2020; Published: 23 September 2020. \n\nFor technical assistance, we thank Fan Gao with the Caltech Bioinformatics Resource Center of the Beckman Institute, Giada Spigolon and Andres Collazo with the Caltech Biological Imaging facility of the Beckman Institute, and Sisi Chen and Paul Rivaud with the Single Cell Profiling and Engineering Center (SPEC) of the Beckman Institute. We thank members of the Bronner lab for helpful discussions. \n\nFunding: National Institutes of Health (R01DE027568) Marianne E Bronner; National Institutes of Health (R01HL14058) Marianne E Bronner; National Institutes of Health (R01DE027538) Marianne E Bronner; American Heart Association (18PRE34050063) Shashank Gandhi; National Institutes of Health (K99DE028592) Erica J Hutchins. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. \n\nAuthor contributions: Shashank Gandhi, Conceptualization, Resources, Software, Formal analysis, Supervision, Validation, Investigation, Visualization, Methodology, Writing - original draft, Writing - review and editing; Erica J Hutchins, Resources, Validation, Investigation, Visualization, Writing - review and editing; Krystyna Maruszko, Investigation, Visualization, Writing - original draft; Jong H Park, Matthew Thomson, Resources, Methodology; Marianne E Bronner, Conceptualization, Supervision, Funding acquisition, Writing - review and editing. \n\nCompeting interests: Marianne E Bronner: Senior editor, eLife. The other authors declare that no competing interests\nexist. \n\nData availability: Sequencing data files have been deposited on NCBI under the accession number PRJNA624258.\n\nPublished - elife-57779-v2.pdf
Accepted Version - elife-57779-v1.pdf
Supplemental Material - elife-57779-supp-v1.zip
Supplemental Material - elife-57779-transrepform-v2.docx
", "abstract": "During gastrulation, neural crest cells are specified at the neural plate border, as characterized by Pax7 expression. Using single-cell RNA sequencing coupled with high resolution in situ hybridization to identify novel transcriptional regulators, we show that chromatin remodeler Hmga1 is highly expressed prior to specification and maintained in migrating chick neural crest cells. Temporally-controlled CRISPR-Cas9-mediated knockouts uncovered two distinct functions of Hmga1 in neural crest development. At the neural plate border, Hmga1 regulates Pax7-dependent neural crest lineage specification. At premigratory stages, a second role manifests where Hmga1 loss reduces cranial crest emigration from the dorsal neural tube independent of Pax7. Interestingly, this is rescued by stabilized \u00df-catenin, thus implicating Hmga1 as a canonical Wnt activator. Together, our results show that Hmga1 functions in a bimodal manner during neural crest development to regulate specification at the neural plate border, and subsequent emigration from the neural tube via canonical Wnt signaling.", "date": "2020-09-23", "date_type": "published", "publication": "eLife", "volume": "2020", "number": "9", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e57779", "id_number": "CaltechAUTHORS:20200929-102516984", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200929-102516984", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R01HL14058" }, { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "American Heart Association", "grant_number": "18PRE34050063" }, { "agency": "NIH", "grant_number": "K99DE028592" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.7554/elife.57779", "pmcid": "PMC7591248", "primary_object": { "basename": "elife-57779-supp-v1.zip", "url": "https://authors.library.caltech.edu/records/wrtcf-y5749/files/elife-57779-supp-v1.zip" }, "related_objects": [ { "basename": "elife-57779-transrepform-v2.docx", "url": "https://authors.library.caltech.edu/records/wrtcf-y5749/files/elife-57779-transrepform-v2.docx" }, { "basename": "elife-57779-v1.pdf", "url": "https://authors.library.caltech.edu/records/wrtcf-y5749/files/elife-57779-v1.pdf" }, { "basename": "elife-57779-v2.pdf", "url": "https://authors.library.caltech.edu/records/wrtcf-y5749/files/elife-57779-v2.pdf" } ], "resource_type": "article", "pub_year": "2020", "author_list": "Gandhi, Shashank; Hutchins, Erica J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zbheh-49r89", "eprint_id": 105491, "eprint_status": "archive", "datestamp": "2023-08-22 06:25:54", "lastmod": "2024-01-15 17:04:56", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A Spectrum of Cell States During the Epithelial-to-Mesenchymal Transition", "ispublished": "unpub", "full_text_status": "public", "keywords": "Epithelial-to-mesenchymal transition; Partial EMT; Cancer; Post-transcriptional regulation; Heterogeneity", "note": "\u00a9 2021 Springer Science+Business Media, LLC, part of Springer Nature. \n\nFirst Online: 17 September 2020.", "abstract": "The epithelial-to-mesenchymal transition (EMT) encompasses a complex cascade of events through which a cell transits to reduce its epithelial characteristics and become migratory. Classically, this transition has been considered complete upon loss of molecular markers characteristic of an \"epithelial\" state and acquisition of those associated with \"mesenchymal\" cells. Recently, however, evidence from both developmental and cancer EMT contexts suggest that cells undergoing EMT are often heterogeneous, concomitantly expressing both epithelial and mesenchymal markers to varying degrees; rather, cells frequently display a \"partial\" EMT phenotype and do not necessarily require full \"mesenchymalization\" to become migratory. Here, we offer a brief perspective on recent important advances in our fundamental understanding of the spectrum of cellular states that occur during partial EMT in the context of development and cancer metastasis.", "date": "2020-09-17", "date_type": "published", "publisher": "Humana Press", "place_of_pub": "New York, NY", "pagerange": "3-6", "id_number": "CaltechAUTHORS:20200923-121742113", "isbn": "978-1-0716-0778-7", "book_title": "The Epithelial-to Mesenchymal Transition: Methods and Protocols", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200923-121742113", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "contributors": { "items": [ { "id": "Campbell-Kyra", "name": { "family": "Campbell", "given": "Kyra" } }, { "id": "Theveneau-E", "name": { "family": "Theveneau", "given": "Eric" } } ] }, "doi": "10.1007/978-1-0716-0779-4_1", "resource_type": "book_section", "pub_year": "2020", "author_list": "Hutchins, Erica J. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/z0kty-e9225", "eprint_id": 104703, "eprint_status": "archive", "datestamp": "2023-08-19 22:49:30", "lastmod": "2023-12-13 16:43:47", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "P-bodies are sites of rapid RNA decay during the neural crest epithelial-mesenchymal transition", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nPosted August 01, 2020. \n\nWe thank A. Collazo and G. Spigolon for imaging assistance at the Caltech Biological Imaging Facility; M. Schwarzkopf and G. Shin (Molecular Technologies) for HCR probe design; S. Manohar, G. da Silva Pescador, and C.J. Andrews for cloning assistance; R. Galton for pilot HCR experiments; and R. Singer, J. Chao, and E. Izaurralde for essential reagents made available via Addgene. This work was supported by the National Institutes of Health [R01DE027538 and R01DE027568 to M.E.B; K99DE028592 to E.J.H; K99DE029240 to M.L.P.]. \n\nData availability: All data generated and/or analyzed during the current study are available from the corresponding author on reasonable request. \n\nCode availability: Information for accessing the JaCoP plugin used to calculate Mander's coefficients is available: https://imagej.nih.gov/ij/plugins/track/jacop.html \n\nAuthor Contributions: Project was conceived by E.J.H. and M.E.B. Experimental design and data interpretation were conducted by E.J.H, M.L.P, and M.E.B. Time-lapse experiments were performed by E.J.H. and M.L.P. Chick electroporations and explants were performed by E.J.H. Constructs were designed and generated by E.J.H. and M.L.P. Embryology, hybridization chain reaction experiments, imaging, and quantitation and statistical analyses were performed by E.J.H. Manuscript was written by E.J.H. and M.E.B., with editing by M.L.P. \n\nThe authors have declared no competing interest.\n\nSubmitted - 2020.07.31.231860v1.full.pdf
", "abstract": "The epithelial-mesenchymal transition (EMT) drives cellular movements during development to create specialized tissues and structures in metazoans, using mechanisms often coopted during metastasis. Neural crest cells are a multipotent stem cell population that undergo a developmentally regulated EMT and are prone to metastasis in the adult, providing an excellent model to study cell state changes and mechanisms underlying EMT. A hallmark of neural crest EMT during avian development is temporally restricted expression followed by rapid down-regulation of the Wnt antagonist Draxin. Using live RNA imaging, here we demonstrate that rapid clearance of Draxin transcripts is mediated post-transcriptionally via localization to processing bodies (P-bodies), small cytoplasmic granules which are established sites of RNA processing. Contrasting with recent work in immortalized cell lines suggesting that P-bodies are sites of storage rather than degradation, we show that targeted decay of Draxin occurs within P-bodies during neural crest migration. Furthermore, P-body disruption via DDX6 knockdown inhibits not only endogenous Draxin down-regulation but also neural crest EMT in vivo. Together, our data highlight a novel and important role for P-bodies in an intact organismal context\u2212controlling a developmental EMT program via post-transcriptional target degradation.", "date": "2020-08-03", "date_type": "published", "id_number": "CaltechAUTHORS:20200803-123033784", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200803-123033784", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027538" }, { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "K99DE028592" }, { "agency": "NIH", "grant_number": "K99DE029240" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2020.07.31.231860", "primary_object": { "basename": "2020.07.31.231860v1.full.pdf", "url": "https://authors.library.caltech.edu/records/z0kty-e9225/files/2020.07.31.231860v1.full.pdf" }, "resource_type": "monograph", "pub_year": "2020", "author_list": "Hutchins, Erica J.; Piacentino, Michael L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0b7fc-7gm94", "eprint_id": 105556, "eprint_status": "archive", "datestamp": "2023-08-19 22:17:05", "lastmod": "2023-12-22 23:14:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Noor-El-Nachef-W", "name": { "family": "Noor El-Nachef", "given": "Wael" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The people behind the papers - Wael Noor El-Nachef and Marianne Bronner", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2020. Published by The Company of Biologists Ltd.\n\nPublished - dev194001.full.pdf
", "abstract": "The enteric nervous system (ENS) derives from the neural crest and innervates the gastrointestinal system, in which it is essential for gut function throughout life. A new paper in Development uses zebrafish to investigate the poorly understood process of post-embryonic ENS neurogenesis, in both development and injury contexts. To find out more, we met the paper's two authors, Wael Noor El-Nachef, Assistant Clinical Professor of Medicine at UCLA, and Marianne Bronner, Albert Billings Ruddock Professor of Biology and Biological Engineering at Caltech.", "date": "2020-07-01", "date_type": "published", "publication": "Development", "volume": "147", "number": "13", "publisher": "Company of Biologists", "pagerange": "Art. No. dev194001", "id_number": "CaltechAUTHORS:20200925-104734996", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200925-104734996", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1242/dev.194001", "primary_object": { "basename": "dev194001.full.pdf", "url": "https://authors.library.caltech.edu/records/0b7fc-7gm94/files/dev194001.full.pdf" }, "resource_type": "article", "pub_year": "2020", "author_list": "Noor El-Nachef, Wael and Bronner, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ne3md-yb348", "eprint_id": 103673, "eprint_status": "archive", "datestamp": "2023-08-22 05:29:20", "lastmod": "2023-12-22 23:14:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "El-Nachef-W-N", "name": { "family": "El-Nachef", "given": "Wael Noor" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "De novo enteric neurogenesis in post-embryonic zebrafish from Schwann cell precursors rather than resident cell types", "ispublished": "pub", "full_text_status": "public", "keywords": "enteric nervous system; neural crest; prucalopride; 5-HT\u2084", "note": "\u00a9 2020 Published by The Company of Biologists Ltd. \n\nReceived November 30, 2019; Accepted June 3, 2020; Published 13 July 2020. \n\nWe thank the Beckman Institute Biological Imaging Facility of Caltech for technical assistance with microscopy experiments. For generously supplying transgenic fish lines, we thank the Ian Shepherd Lab (Phox2b-kaede), the Jua-Nian Chen Lab (sox10:GAL4-UAS-Cre and ubi:switch) and the Christiane N\u00fcsslein-Volhard Lab (cmlc:GFP-sox10:ERT2-Cre). Special thanks to Megan Martik and Can Li for sharing ISH probes (Sox10, Phox2bb). We also recognize Claire Hu (California Institute of Technology) for assistance with IHC. \n\nThe authors declare no competing or financial interests. \n\nAuthor contributions: Conceptualization: W.N.E.-N., M.E.B.; Methodology: W.N.E.-N., M.E.B.; Validation: W.N.E.-N.; Formal analysis: W.N.E.-N., M.E.B.; Investigation: W.N.E.-N.; Resources: W.N.E.-N., M.E.B.; Data curation: W.N.E.-N., M.E.B.; Writing - original draft: W.N.E.-N.; Writing - review & editing: W.N.E.-N., M.E.B.; Visualization: W.N.E.-N.; Supervision: M.E.B.; Project administration: M.E.B.; Funding acquisition: M.E.B. \n\nThis work was supported by the National Institutes of Health (NIH R35NS111564 to M.E.B. and NIH R01NS108500 to M.E.B.). Deposited in PMC for release after 12 months.\n\nPublished - dev186619.full.pdf
Submitted - 2020.06.01.127712v1.full.pdf
Supplemental Material - DEV186619supp.pdf
Supplemental Material - media-1.docx
Supplemental Material - media-2.mp4
Supplemental Material - media-3.mp4
Supplemental Material - media-4.mp4
Supplemental Material - media-5.avi
Supplemental Material - media-6.avi
", "abstract": "The enteric nervous system (ENS) is essential for normal gastrointestinal function. Although the embryonic origin of enteric neurons from the neural crest is well established, conflicting evidence exists regarding postnatal enteric neurogenesis. Here, we address this by examining the origin of de novo neurogenesis in the post-embryonic zebrafish ENS. Although new neurons are added during growth and after injury, the larval intestine appears to lack resident neurogenic precursors or classical glia marked by sox10, plp1a, gfap or s100. Rather, lineage tracing with lipophilic dye or inducible Sox10-Cre suggests that post-embryonic enteric neurons arise from trunk neural crest-derived Schwann cell precursors that migrate from the spinal cord into the intestine. Furthermore, the 5-HT\u2084 receptor agonist prucalopride increases enteric neurogenesis in normal development and after injury. Taken together, the results suggest that despite the lack of resident progenitors in the gut, post-embryonic enteric neurogenesis occurs via gut-extrinsic Schwann cell precursors during development and injury, and is promoted by serotonin receptor agonists. The absence of classical glia in the ENS further suggests that neural crest-derived enteric glia might have evolved after the teleost lineage.", "date": "2020-07", "date_type": "published", "publication": "Development", "volume": "147", "number": "13", "publisher": "Company of Biologists", "pagerange": "Art. No. dev186619", "id_number": "CaltechAUTHORS:20200603-132940388", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200603-132940388", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "NIH", "grant_number": "R01NS108500" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1242/dev.186619", "pmcid": "PMC7375481", "primary_object": { "basename": "dev186619.full.pdf", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/dev186619.full.pdf" }, "related_objects": [ { "basename": "2020.06.01.127712v1.full.pdf", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/2020.06.01.127712v1.full.pdf" }, { "basename": "DEV186619supp.pdf", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/DEV186619supp.pdf" }, { "basename": "media-2.mp4", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-2.mp4" }, { "basename": "media-3.mp4", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-3.mp4" }, { "basename": "media-4.mp4", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-4.mp4" }, { "basename": "media-5.avi", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-5.avi" }, { "basename": "media-6.avi", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-6.avi" }, { "basename": "media-1.docx", "url": "https://authors.library.caltech.edu/records/ne3md-yb348/files/media-1.docx" } ], "resource_type": "article", "pub_year": "2020", "author_list": "El-Nachef, Wael Noor and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/aq5t1-ed459", "eprint_id": 102660, "eprint_status": "archive", "datestamp": "2023-08-22 05:11:34", "lastmod": "2023-12-22 23:19:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Yang-Jing", "name": { "family": "Yang", "given": "Jing" }, "orcid": "0000-0001-8410-3549" }, { "name": { "family": "Antin", "given": "Parker" } }, { "name": { "family": "Berx", "given": "Geert" } }, { "name": { "family": "Blanpain", "given": "C\u00e9dric" } }, { "name": { "family": "Brabletz", "given": "Thomas" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "name": { "family": "Campbell", "given": "Kyra" } }, { "name": { "family": "Cano", "given": "Amparo" } }, { "name": { "family": "Casanova", "given": "Jordi" } }, { "name": { "family": "Christofori", "given": "Gerhard" } }, { "name": { "family": "Dedhar", "given": "Shoukat" } }, { "name": { "family": "Derynck", "given": "Rik" } }, { "name": { "family": "Ford", "given": "Heide L." } }, { "name": { "family": "Fuxe", "given": "Jonas" }, "orcid": "0000-0003-4576-9377" }, { "name": { "family": "Garc\u00eda\u00a0de\u00a0Herreros", "given": "Antonio" } }, { "name": { "family": "Goodall", "given": "Gregory J." } }, { "name": { "family": "Hadjantonakis", "given": "Anna-Katerina" } }, { "name": { "family": "Huang", "given": "Ruby J. Y." } }, { "name": { "family": "Kalcheim", "given": "Chaya" } }, { "name": { "family": "Kalluri", "given": "Raghu" } }, { "name": { "family": "Kang", "given": "Yibin" }, "orcid": "0000-0002-1626-6730" }, { "name": { "family": "Khew-Goodall", "given": "Yeesim" } }, { "name": { "family": "Levine", "given": "Herbert" } }, { "name": { "family": "Liu", "given": "Jinsong" } }, { "name": { "family": "Longmore", "given": "Gregory D." } }, { "name": { "family": "Mani", "given": "Sendurai A." } }, { "name": { "family": "Massagu\u00e9", "given": "Joan" } }, { "name": { "family": "Mayor", "given": "Roberto" }, "orcid": "0000-0001-9053-9613" }, { "name": { "family": "McClay", "given": "David" } }, { "name": { "family": "Mostov", "given": "Keith E." }, "orcid": "0000-0002-8123-6247" }, { "name": { "family": "Newgreen", "given": "Donald F." }, "orcid": "0000-0002-3467-6389" }, { "name": { "family": "Nieto", "given": "M. Angela" }, "orcid": "0000-0002-3538-840X" }, { "name": { "family": "Puisieux", "given": "Alain" }, "orcid": "0000-0002-9938-3798" }, { "name": { "family": "Runyan", "given": "Raymond" }, "orcid": "0000-0002-7410-916X" }, { "name": { "family": "Savagner", "given": "Pierre" } }, { "name": { "family": "Stanger", "given": "Ben" } }, { "name": { "family": "Stemmler", "given": "Marc P." }, "orcid": "0000-0002-7866-3686" }, { "name": { "family": "Takahashi", "given": "Yoshiko" } }, { "name": { "family": "Takeichi", "given": "Masatoshi" }, "orcid": "0000-0002-9931-3378" }, { "name": { "family": "Theveneau", "given": "Eric" }, "orcid": "0000-0001-6510-5717" }, { "name": { "family": "Thiery", "given": "Jean Paul" }, "orcid": "0000-0003-0478-5020" }, { "name": { "family": "Thompson", "given": "Erik W." }, "orcid": "0000-0002-9723-4924" }, { "name": { "family": "Weinberg", "given": "Robert A." } }, { "name": { "family": "Williams", "given": "Elizabeth D." }, "orcid": "0000-0002-3364-6655" }, { "name": { "family": "Xing", "given": "Jianhua" }, "orcid": "0000-0002-3700-8765" }, { "name": { "family": "Zhou", "given": "Binhua P." } }, { "name": { "family": "Sheng", "given": "Guojun" } } ] }, "title": "Guidelines and definitions for research on epithelial\u2013mesenchymal transition", "ispublished": "pub", "full_text_status": "public", "keywords": "Cancer; Developmental biology; Epithelial\u2013mesenchymal transition", "note": "\u00a9 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. \n\nAccepted 11 March 2020; Published 16 April 2020. \n\nWe sincerely apologize to the many researchers whose work we were unable to cite due to space restrictions. \n\nAuthor Contributions: All authors contributed to the discussion of content and to the writing and/or revision of the article. \n\nThe authors declare no competing interests.\n\nPublished - s41580-020-0237-9.pdf
Supplemental Material - 41580_2020_237_MOESM1_ESM.pdf
", "abstract": "Epithelial\u2013mesenchymal transition (EMT) encompasses dynamic changes in cellular organization from epithelial to mesenchymal phenotypes, which leads to functional changes in cell migration and invasion. EMT occurs in a diverse range of physiological and pathological conditions and is driven by a conserved set of inducing signals, transcriptional regulators and downstream effectors. With over 5,700 publications indexed by Web of Science in 2019 alone, research on EMT is expanding rapidly. This growing interest warrants the need for a consensus among researchers when referring to and undertaking research on EMT. This Consensus Statement, mediated by 'the EMT International Association' (TEMTIA), is the outcome of a 2-year-long discussion among EMT researchers and aims to both clarify the nomenclature and provide definitions and guidelines for EMT research in future publications. We trust that these guidelines will help to reduce misunderstanding and misinterpretation of research data generated in various experimental models and to promote cross-disciplinary collaboration to identify and address key open questions in this research field. While recognizing the importance of maintaining diversity in experimental approaches and conceptual frameworks, we emphasize that lasting contributions of EMT research to increasing our understanding of developmental processes and combatting cancer and other diseases depend on the adoption of a unified terminology to describe EMT.", "date": "2020-06", "date_type": "published", "publication": "Nature Reviews. Molecular Cell Biology", "volume": "21", "number": "6", "publisher": "Nature Publishing Group", "pagerange": "341-352", "id_number": "CaltechAUTHORS:20200420-125839736", "issn": "1471-0072", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200420-125839736", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1038/s41580-020-0237-9", "primary_object": { "basename": "41580_2020_237_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/aq5t1-ed459/files/41580_2020_237_MOESM1_ESM.pdf" }, "related_objects": [ { "basename": "s41580-020-0237-9.pdf", "url": "https://authors.library.caltech.edu/records/aq5t1-ed459/files/s41580-020-0237-9.pdf" } ], "resource_type": "article", "pub_year": "2020", "author_list": "Yang, Jing; Antin, Parker; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jx0b3-d9d14", "eprint_id": 102994, "eprint_status": "archive", "datestamp": "2023-08-22 04:59:29", "lastmod": "2023-12-22 23:19:12", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Ezin-Max", "name": { "family": "Ezin", "given": "Max" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Reprogramming Axial Level Identity to Rescue Neural-Crest-Related Congenital Heart Defects", "ispublished": "pub", "full_text_status": "public", "keywords": "cardiac neural crest; heart development; aorticopulmonary septum; persistent truncus arteriosus; outflow tract; reprogramming; specification; cardiac crest subcircuit; congenital birth defects; ectomesenchymal fate", "note": "\u00a9 2020 Elsevier Inc. \n\nReceived 2 October 2019, Revised 7 February 2020, Accepted 6 April 2020, Available online 4 May 2020. \n\nWe thank Drs. Tatjana Sauka-Spengler and Megan Martik for helpful discussions. For technical assistance, we thank Jamie Tijerina and Rochelle Diamond with the Beckman Institute (BI) Flow Cytometry Facility, Igor Antoshechkin with the Jacobs Genetics and Genomics Laboratory, Giada Spigolon and Andres Collazo with the BI Biological Imaging Facility, and Fan Gao with the BI Bioinformatics Resource Center. We thank Neil Ashley and Ivan Candido Ferreira at the Weatherall Institute (University of Oxford) for help with scRNA-seq library preparation and sequencing. This work was supported by NIH grants R01DE027568 and R01HL14058 to M.E.B. and AHA predoctoral fellowship 18PRE34050063 and Company of Biologists traveling fellowship DEVTF18119 to S.G. \n\nAuthor Contributions: Conceptualization, S.G. and M.E.B.; Methodology, S.G. and M.E.; Software, S.G.; Validation, S.G; Formal Analysis, S.G.; Investigation, S.G. and M.E.; Writing \u2013 Original Draft, S.G., M.E., and M.E.B.; Writing \u2013 Review & Editing, S.G. and M.E.B.; Visualization, S.G.; Supervision, M.E.B.; Funding Acquisition, M.E.B. \n\nThe authors declare no competing interests.\n\nAccepted Version - nihms-1588541.pdf
Supplemental Material - 1-s2.0-S1534580720302720-mmc1.pdf
Supplemental Material - 1-s2.0-S1534580720302720-mmc2.xlsx
Supplemental Material - 1-s2.0-S1534580720302720-mmc3.xlsx
", "abstract": "The cardiac neural crest arises in the hindbrain, then migrates to the heart and contributes to critical structures, including the outflow tract septum. Chick cardiac crest ablation results in failure of this septation, phenocopying the human heart defect persistent truncus arteriosus (PTA), which trunk neural crest fails to rescue. Here, we probe the molecular mechanisms underlying the cardiac crest's unique potential. Transcriptional profiling identified cardiac-crest-specific transcription factors, with single-cell RNA sequencing revealing surprising heterogeneity, including an ectomesenchymal subpopulation within the early migrating population. Loss-of-function analyses uncovered a transcriptional subcircuit, comprised of Tgif1, Ets1, and Sox8, critical for cardiac neural crest and heart development. Importantly, ectopic expression of this subcircuit was sufficient to imbue trunk crest with the ability to rescue PTA after cardiac crest ablation. Together, our results reveal a transcriptional program sufficient to confer cardiac potential onto trunk neural crest cells, thus implicating new genes in cardiovascular birth defects.", "date": "2020-05-04", "date_type": "published", "publication": "Developmental Cell", "volume": "53", "number": "3", "publisher": "Cell Press", "pagerange": "300-315", "id_number": "CaltechAUTHORS:20200505-080826421", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200505-080826421", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R01HL14058" }, { "agency": "American Heart Association", "grant_number": "18PRE34050063" }, { "agency": "Company of Biologists", "grant_number": "DEVTF18119" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1016/j.devcel.2020.04.005", "pmcid": "PMC7255058", "primary_object": { "basename": "1-s2.0-S1534580720302720-mmc1.pdf", "url": "https://authors.library.caltech.edu/records/jx0b3-d9d14/files/1-s2.0-S1534580720302720-mmc1.pdf" }, "related_objects": [ { "basename": "1-s2.0-S1534580720302720-mmc2.xlsx", "url": "https://authors.library.caltech.edu/records/jx0b3-d9d14/files/1-s2.0-S1534580720302720-mmc2.xlsx" }, { "basename": "1-s2.0-S1534580720302720-mmc3.xlsx", "url": "https://authors.library.caltech.edu/records/jx0b3-d9d14/files/1-s2.0-S1534580720302720-mmc3.xlsx" }, { "basename": "nihms-1588541.pdf", "url": "https://authors.library.caltech.edu/records/jx0b3-d9d14/files/nihms-1588541.pdf" } ], "resource_type": "article", "pub_year": "2020", "author_list": "Gandhi, Shashank; Ezin, Max; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8h1zm-zwc94", "eprint_id": 100038, "eprint_status": "archive", "datestamp": "2023-08-22 04:08:27", "lastmod": "2023-12-22 23:25:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mehrotra-P", "name": { "family": "Mehrotra", "given": "Pihu" }, "orcid": "0000-0001-9369-5013" }, { "id": "Tseropoulos-Georgios", "name": { "family": "Tseropoulos", "given": "Georgios" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Andreadis-Stelios-T", "name": { "family": "Andreadis", "given": "Stelios T." } } ] }, "title": "Adult tissue-derived neural crest\u2010like stem cells: Sources, regulatory networks, and translational potential: Concise review", "ispublished": "pub", "full_text_status": "public", "keywords": "demyelinating disorders; gene regulatory network; neural crest; Schwann cells", "note": "\u00a9 2019 The Authors. Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. \n\nVersion of Record online: 18 November 2019; Manuscript accepted: 25 October 2019; Manuscript revised: 22 October 2019; Manuscript received: 17 June 2019. \n\nThis work was supported by grants from the National Institutes of Health R01 EB023114 (S.T.A.) and the New York Stem Cell Science NYSTEM (Contract #C30290GG, S.T.A.). \n\nThe authors declare no potential conflict of interest. \n\nAuthor Contributions: Pihu Mehrotra\u2010 wrote the manuscript, specifically parts on introduction to the topic, sources of neural crest and their genetic regulation, their application for treatment of demyelinating disorders and made the manuscript figures. Georgios Tseropoulos\u2010 wrote the manuscript, specifically mechanisms underlying differentiation of neural crest cells to Schwann cells and made the manuscript figures. Marianne E. Bronner\u2010 wrote the manuscript, specifically content on how Schwann cell precursors contribute to neural crest derivatives, and gene regulatory networks controlling lineage diversification. Stelios T. Andreadis\u2010 conceived overall review content, writing and editing the manuscript and project supervision. \n\nData Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.\n\nPublished - Mehrotra_et_al-2020-STEM_CELLS_Translational_Medicine.pdf
Supplemental Material - sct312630-sup-0001-supinfo.pdf
", "abstract": "Neural crest (NC) cells are a multipotent stem cell population that gives rise to a diverse array of cell types in the body, including peripheral neurons, Schwann cells (SC), craniofacial cartilage and bone, smooth muscle cells, and melanocytes. NC formation and differentiation into specific lineages takes place in response to a set of highly regulated signaling and transcriptional events within the neural plate border. Pre\u2010migratory NC cells initially are contained within the dorsal neural tube from which they subsequently emigrate, migrating to often distant sites in the periphery. Following their migration and differentiation, some NC\u2010like cells persist in adult tissues in a nascent multipotent state, making them potential candidates for autologous cell therapy. This review discusses the gene regulatory network responsible for NC development and maintenance of multipotency. We summarize the genes and signaling pathways that have been implicated in the differentiation of a post\u2010migratory NC into mature myelinating SC. We elaborate on the signals and transcription factors involved in the acquisition of immature SC fate, axonal sorting of unmyelinated neuronal axons, and finally the path toward mature myelinating SC, which envelope axons within myelin sheaths, facilitating electrical signal propagation. The gene regulatory events guiding development of SC in\u2010vivo provides insights into means for differentiating NC\u2010like cells from adult human tissues into functional SC, which have the potential to provide autologous cell sources for the treatment of demyelinating and neurodegenerative disorders.", "date": "2020-03", "date_type": "published", "publication": "Stem Cells Translational Medicine", "volume": "9", "number": "3", "publisher": "AlphaMed Press", "pagerange": "328-341", "id_number": "CaltechAUTHORS:20191125-135819310", "issn": "2157-6564", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20191125-135819310", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 EB023114" }, { "agency": "New York Stem Cell Science (NYSTEM)", "grant_number": "C30290GG" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1002/sctm.19-0173", "primary_object": { "basename": "sct312630-sup-0001-supinfo.pdf", "url": "https://authors.library.caltech.edu/records/8h1zm-zwc94/files/sct312630-sup-0001-supinfo.pdf" }, "related_objects": [ { "basename": "Mehrotra_et_al-2020-STEM_CELLS_Translational_Medicine.pdf", "url": "https://authors.library.caltech.edu/records/8h1zm-zwc94/files/Mehrotra_et_al-2020-STEM_CELLS_Translational_Medicine.pdf" } ], "resource_type": "article", "pub_year": "2020", "author_list": "Mehrotra, Pihu; Tseropoulos, Georgios; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jd91f-d0376", "eprint_id": 102461, "eprint_status": "archive", "datestamp": "2023-08-19 20:21:03", "lastmod": "2023-12-22 23:19:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "El-Nachef-W", "name": { "family": "El-Nachef", "given": "Wael" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "De novo neurogenesis in the post-embryonic zebrafish enteric nervous system from Schwann cell precursors rather than resident cell types", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2020 The Authors. Neurogastroenterology & Motility \u00a9 2020 John Wiley & Sons Ltd. \n\nIssue Online: 22 March 2020; Version of Record online: 22 March 2020.", "abstract": "The enteric nervous system (ENS) is essential for normal gastrointestinal motility, and defects in its function define several difficult to treat conditions. While the developmental origin of the ENS from neural crest cells is well understood, there is conflicting evidence regarding postnatal enteric neurogenesis and neuronal homeostasis. Using zebrafish as a model due to its simplified ENS which is amenable to live-imaging, we sought to explore the origin of enteric neurons that arise in post-embryonic life in both normal development and upon injury, and tested effects of the 5-HT\u2084 receptor agonist, prucalopride, in this process.", "date": "2020-03", "date_type": "published", "publication": "Neurogastroenterology and Motility", "volume": "32", "number": "S1", "publisher": "Wiley", "pagerange": "Art. No. 29", "id_number": "CaltechAUTHORS:20200409-140201559", "issn": "1350-1925", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200409-140201559", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1111/nmo.13817", "resource_type": "article", "pub_year": "2020", "author_list": "El-Nachef, Wael and Bronner, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rkcyf-x5f45", "eprint_id": 100869, "eprint_status": "archive", "datestamp": "2023-08-19 19:39:59", "lastmod": "2023-12-13 16:44:54", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mohlin-Sofie", "name": { "family": "Mohlin", "given": "Sofie" }, "orcid": "0000-0002-2458-3963" }, { "id": "Persson-Camilla-U", "name": { "family": "Persson", "given": "Camilla U." } }, { "id": "Fredlund-E", "name": { "family": "Fredlund", "given": "Elina" } }, { "id": "Monni-E", "name": { "family": "Monni", "given": "Emanuela" } }, { "id": "Lindvall-J-M", "name": { "family": "Lindvall", "given": "Jessica M." } }, { "id": "Kokaia-Z", "name": { "family": "Kokaia", "given": "Zaal" } }, { "id": "Hammarlund-E", "name": { "family": "Hammarlund", "given": "Emma" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cancer-associated HIF-2\u03b1 impacts trunk neural crest stemness", "ispublished": "unpub", "full_text_status": "public", "keywords": "Neural crest, trunk neural crest, neuroblastoma, hypoxia inducible factor-2, HIF, chick embryo", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nbioRxiv preprint first posted online Jan. 23, 2020. \n\nWe would like to thank Erica Hutchins, Shashank Gandhi and Siv Beckman for skillful technical assistance and Anni Glud and Ronnie N. Glud for providing microsensor technique and expertise. This work was supported by the Swedish Cancer Society, the Swedish Childhood Cancer Fund, the Craaford Foundation, Jeansson Foundations, Ollie and Elof Ericsson's foundation, the Mary Bev\u00e9 Foundation, Magnus Bergvall's foundation, the Thelma Zoega\nfoundation for medical research, Hans von Kantzow's foundation, the Royal Physiographic Society of Lund, the Gyllenstierna Krapperup's Foundation, and Gunnar Nilssons Cancerstiftelse (to SM), DE027568 and R01HL14058 (to MEB). We thank Center for Translational Genomics, Lund University and Clinical Genomics Lund, SciLifeLab for providing sequencing service. Support by NBIS (National Bioinformatics Infrastructure Sweden) is gratefully acknowledged. \n\nAuthor contributions: SM, CUP, EF and EH performed experiments. SM, EH and MEB analyzed data. SM and JML analyzed RNA sequencing data. EM and ZK provided materials. SM and MEB supervised the study. SM wrote the original draft of the manuscript while all authors reviewed and edited the manuscript. \n\nThe authors declare no competing interests.\n\nSubmitted - 2020.01.22.915199v1.full.pdf
", "abstract": "The neural crest is a stem cell population that gives rise to sympathetic ganglia, the cell type of origin of neuroblastoma. Hypoxia Inducible Factor (HIF)-2\u03b1 is associated with high risk neuroblastoma, however, little is known about its role in normal neural crest development. To address this important question, here we show that HIF-2\u03b1 is expressed in trunk neural crest cells of human, murine and avian embryos. Modulating HIF-2\u03b1 in vivo not only causes developmental delays but also induces proliferation and stemness of neural crest cells while altering the number of cells migrating ventrally to sympathoadrenal sites. Transcriptome changes after loss of HIF-2\u03b1 reflect the in vivo phenotype. The results suggest that expression levels of HIF-2\u03b1 must be strictly controlled and abnormal levels increase stemness and may promote metastasis. Our findings help elucidate the role of HIF-2\u03b1 during normal development with implications also in tumor initiation at the onset of neuroblastoma.", "date": "2020-01-23", "date_type": "published", "id_number": "CaltechAUTHORS:20200123-094934019", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200123-094934019", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Swedish Cancer Society" }, { "agency": "Swedish Childhood Cancer Fund" }, { "agency": "Craaford Foundation" }, { "agency": "Jeansson Foundations" }, { "agency": "Ollie and Elof Ericssons Foundation" }, { "agency": "Mary Bev\u00e9 Foundation" }, { "agency": "Magnus Bergvall Foundation" }, { "agency": "Thelma Zo\u00e9ga Foundation" }, { "agency": "Hans von Kantzow's Foundation" }, { "agency": "Royal Physiographic Society of Lund" }, { "agency": "Gyllenstierna Krapperup's Foundation" }, { "agency": "Gunnar Nilsson Cancer Foundation" }, { "agency": "NIH", "grant_number": "DE027568" }, { "agency": "NIH", "grant_number": "R01HL14058" }, { "agency": "National Bioinformatics Infrastructure Sweden" } ] }, "local_group": { "items": [ { "id": "Division-of-Biology-and-Biological-Engineering" } ] }, "doi": "10.1101/2020.01.22.915199", "primary_object": { "basename": "2020.01.22.915199v1.full.pdf", "url": "https://authors.library.caltech.edu/records/rkcyf-x5f45/files/2020.01.22.915199v1.full.pdf" }, "resource_type": "monograph", "pub_year": "2020", "author_list": "Mohlin, Sofie; Persson, Camilla U.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zwnd3-vgm96", "eprint_id": 98650, "eprint_status": "archive", "datestamp": "2023-08-22 02:47:38", "lastmod": "2023-10-18 17:31:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Ghandi-Shashank", "name": { "family": "Ghandi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Uy-Benjamin-R", "name": { "family": "Uy", "given": "Benjamin R." }, "orcid": "0000-0003-0438-880X" }, { "id": "Gillis-J-Andrew", "name": { "family": "Gillis", "given": "J. Andrew" }, "orcid": "0000-0003-2062-3777" }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolution of the new head by gradual acquisition of neural crest regulatory circuits", "ispublished": "pub", "full_text_status": "public", "keywords": "Embryology; Evolutionary developmental biology", "note": "\u00a9 2019 Springer Nature Limited. \n\nReceived 22 October 2018; Accepted 26 September 2019; Published 23 October 2019. \n\nData availability: All raw sequencing data for all RNA-seq libraries (Fig. 3) and merged reference transcriptomes are available online (NCBI BioProject# PRJNA497902). Sequences of in situ probe templates for Figs. 1b, c, 2a, c are available through GenBank accession codes (see Methods). \n\nCode availability: Code used to analyse sequencing datasets are available from the corresponding author upon request. \n\nWe thank J. Tan-Cabugao and E. Grossman for technical assistance; D. Mayorga and R. Fraser for help with fish husbandry; B. Martik for illustrating the adult animals for our expression matrices; the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory and in particular I. Antoshechkin for sequencing of our RNA-seq libraries; and R. Diamond, J. Tijerina, D. Perez, and P. Cannon of the The Caltech Flow Cytometry Cell Sorting Facility for cell sorting assistance. This work is supported by NIH grants R01NS086907, R01DE024157, and R35NS111564 to M.E.B. M.L.M. is supported by a Helen Hay Whitney Foundation postdoctoral fellowship. S.G. is supported by a graduate fellowship from the American Heart Association (18PRE34050063). \n\nAuthor Contributions: Project and analysis conception were designed by M.L.M., M.S.-C., and M.E.B. Writing and interpretation were performed by M.L.M., S.G., B.R.U., J.A.G., S.A.G., M.S.-C., and M.E.B. Lamprey orthologue cloning and all in situ hybridization, imaging, and analysis were performed by M.L.M. Bioinformatics and chicken RNA-seq were performed by S.G. Phylogenetic analysis and lamprey embryo acquisition were performed by S.A.G. Cloning of skate orthologues and skate embryo acquisition were performed by J.A.G. Lamprey embryo dissections and library preparations were performed by B.R.U. and M.S.C. \n\nThe authors declare no competing interests.\n\nAccepted Version - nihms-1540804.pdf
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", "abstract": "The neural crest, an embryonic stem-cell population, is a vertebrate innovation that has been proposed to be a key component of the 'new head', which imbued vertebrates with predatory behaviour. Here, to investigate how the evolution of neural crest cells affected the vertebrate body plan, we examined the molecular circuits that control neural crest development along the anteroposterior axis of a jawless vertebrate, the sea lamprey. Gene expression analysis showed that the cranial subpopulation of the neural crest of the lamprey lacks most components of a transcriptional circuit that is specific to the cranial neural crest in amniotes and confers the ability to form craniofacial cartilage onto non-cranial neural crest subpopulations3. Consistent with this, hierarchical clustering analysis revealed that the transcriptional profile of the lamprey cranial neural crest is more similar to the trunk neural crest of amniotes. Notably, analysis of the cranial neural crest in little skate and zebrafish embryos demonstrated that the transcriptional circuit that is specific to the cranial neural crest emerged via the gradual addition of network components to the neural crest of gnathostomes, which subsequently became restricted to the cephalic region. Our results indicate that the ancestral neural crest at the base of the vertebrate lineage possessed a trunk-like identity. We propose that the emergence of the cranial neural crest, by progressive assembly of an axial-specific regulatory circuit, allowed the elaboration of the new head during vertebrate evolution.", "date": "2019-10-31", "date_type": "published", "publication": "Nature", "volume": "574", "number": "7780", "publisher": "Nature Publishing Group", "pagerange": "675-678", "id_number": "CaltechAUTHORS:20190916-095004029", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190916-095004029", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01NS086907" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH", "grant_number": "R35NS111564" }, { "agency": "Helen Hay Whitney Foundation" }, { "agency": "American Heart Association", "grant_number": "18PRE34050063" } ] }, "doi": "10.1038/s41586-019-1691-4", "pmcid": "PMC6858584", "primary_object": { "basename": "41586_2019_1691_Fig7_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig7_ESM.jpg" }, "related_objects": [ { "basename": "41586_2019_1691_Fig9_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig9_ESM.jpg" }, { "basename": "41586_2019_1691_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_MOESM1_ESM.pdf" }, { "basename": "41586_2019_1691_MOESM2_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_MOESM2_ESM.xlsx" }, { "basename": "nihms-1540804.pdf", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/nihms-1540804.pdf" }, { "basename": "41586_2019_1691_Fig10_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig10_ESM.jpg" }, { "basename": "41586_2019_1691_Fig5_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig5_ESM.jpg" }, { "basename": "41586_2019_1691_Fig6_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig6_ESM.jpg" }, { "basename": "41586_2019_1691_Fig8_ESM.jpg", "url": "https://authors.library.caltech.edu/records/zwnd3-vgm96/files/41586_2019_1691_Fig8_ESM.jpg" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Martik, Megan L.; Ghandi, Shashank; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zeg8d-38t27", "eprint_id": 90438, "eprint_status": "archive", "datestamp": "2023-08-22 02:43:44", "lastmod": "2023-10-23 17:02:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hockman-D", "name": { "family": "Hockman", "given": "Dorit" }, "orcid": "0000-0003-2613-6216" }, { "id": "Chong-Morrison-V", "name": { "family": "Chong-Morrison", "given": "Vanessa" }, "orcid": "0000-0002-2547-3694" }, { "id": "Green-S-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Gavriouchkina-D", "name": { "family": "Gavriouchkina", "given": "Daria" }, "orcid": "0000-0002-7488-5898" }, { "id": "Candido-Ferreira-I", "name": { "family": "Candido-Ferreira", "given": "Ivan" } }, { "id": "Ling-Irving-T-C", "name": { "family": "Ling", "given": "Irving T. C." } }, { "id": "Williams-R-M", "name": { "family": "Williams", "given": "Ruth M." } }, { "id": "Amemiya-C-T", "name": { "family": "Amemiya", "given": "Chris T." } }, { "id": "Smith-J-J", "name": { "family": "Smith", "given": "Jeramiah J." }, "orcid": "0000-0001-5333-5531" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "A genome-wide assessment of the ancestral neural crest gene regulatory network", "ispublished": "pub", "full_text_status": "public", "keywords": "Epigenomics; Evolutionary developmental biology; Regulatory networks; Transcriptomics", "note": "\u00a9 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.\n\nReceived 03 March 2018; Accepted\n23 September 2019; Published 16 October 2019.\n\nData availability:\nThe authors declare that all data supporting the findings of this study are available within the article and its supplementary information files or from the corresponding author upon reasonable request. The data sets generated during and/or analysed during the current study have been deposited in the NCBI GEOarchive database under accession code: GSE112072 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE112072.\n\nCode availability:\nThe custom script used to calculate motif co-occurrences is available at https://github.com/tsslab/chick_NC-GRN. Details of software versions and parameters used, when different from default, are indicated in the Methods section.\n\nWe thank Sebastian Shimeld for access to embryos and genomic DNA of the brook lamprey, Hugo Parker for the lamprey HLC vector, and Sally-Ann Clark at the WIMM FACS Core Facility for assistance with cell sorting. We thank Toros Tasgin and Sarah Meyes for cloning candidate genes for in situ and Justine Van Greenen for amplifying and analysing L. planeri genomic DNA sequences. This work was supported by a Leverhulme Research Grant to T.S.S. (RPG-2015\u2013026), the National Institute of General Medical Sciences of the National Institutes of Health grants to J.J.S. (R01GM104123) and C.T.A. (R24GM095471), a Wellcome Trust Institutional Strategic Support Fund grant (H2RZKC00) to D.H. and T.S.S., a Junior Research Fellowship (Trinity College, Oxford), the Sydney Brenner Fellowship, a Company of Biologists Travelling Fellowship (DEVTF-150403) and an EMBO Short Term Fellowship to D.H., and a Clarendon Fund Fellowship to V.C.M.\n\nAuthor Contributions:\nD.H. and T.S.S. conceived this research programme. D.H. generated RNA-seq and ATAC-seq data, performed and analysed lamprey reporter expression assays and performed bioinformatics analysis. V.C.-M. performed zebrafish transgenesis, splinkerette assay, CRIPSR/Cas9 experiments and immunostaining. S.G. performed lamprey whole-mount in situ hybridisation. D.G. assisted in the analysis of RNA-seq and ATAC-seq data. I.C.F assisted in ATAC-seq data analysis. I.L. performed chicken embryo electroporations and imaging. R.W. performed in situ HCR and whole-mount in situ hybridisations. J.S. and C.T.A. provided access to the draft sea lamprey germline genome assembly. M.E.B. provided access to sea lamprey embryos. D.H. and T.S.S. discussed ideas and interpretations and wrote the manuscript. D.H., M.E.B., and T.S.S. edited the manuscript and all authors commented on it. T.S.S. supervised the study.\n\nCompeting Interests:\nThe authors declare no competing interests.\n\nPublished - s41467-019-12687-4.pdf
Submitted - 275875.full.pdf
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", "abstract": "The neural crest is an embryonic cell population that contributes to key vertebrate-specific features including the craniofacial skeleton and peripheral nervous system. Here we examine the transcriptional profiles and chromatin accessibility of neural crest cells in the basal sea lamprey, in order to gain insight into the ancestral state of the neural crest gene regulatory network (GRN) at the dawn of vertebrates. Transcriptome analyses reveal clusters of co-regulated genes during neural crest specification and migration that show high conservation across vertebrates for dynamic programmes like Wnt modulation during the epithelial to mesenchymal transition, but also reveal novel transcription factors and cell-adhesion molecules not previously implicated in neural crest migration. ATAC-seq analysis refines the location of known cis-regulatory elements at the Hox-\u03b12 locus and uncovers novel cis-regulatory elements for Tfap2B and SoxE1. Moreover, cross-species deployment of lamprey elements in zebrafish reveals that the lamprey SoxE1 enhancer activity is deeply conserved, mediating homologous expression in jawed vertebrates. Together, our data provide new insight into the core elements of the GRN that are conserved to the base of the vertebrates, as well as expose elements that are unique to lampreys.", "date": "2019-10-16", "date_type": "published", "publication": "Nature Communications", "volume": "10", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 4689", "id_number": "CaltechAUTHORS:20181026-140937942", "issn": "2041-1723", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20181026-140937942", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Leverhulme Trust", "grant_number": "RPG-2015-026" }, { "agency": "NIH", "grant_number": "R01GM104123" }, { "agency": "NIH", "grant_number": "R24GM095471" }, { "agency": "Wellcome Trust", "grant_number": "H2RZKC00" }, { "agency": "Trinity College" }, { "agency": "Sydney Brenner Fellowship" }, { "agency": "Company of Biologists", "grant_number": "DEVTF-150403" }, { "agency": "European Molecular Biology Organization (EMBO)" }, { "agency": "Clarendon Fund" } ] }, "doi": "10.1038/s41467-019-12687-4", "pmcid": "PMC6795873", "primary_object": { "basename": "275875.full.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/275875.full.pdf" }, "related_objects": [ { "basename": "41467_2019_12687_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM1_ESM.pdf" }, { "basename": "41467_2019_12687_MOESM3_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM3_ESM.xlsx" }, { "basename": "41467_2019_12687_MOESM4_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM4_ESM.xlsx" }, { "basename": "41467_2019_12687_MOESM5_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM5_ESM.xlsx" }, { "basename": "41467_2019_12687_MOESM6_ESM.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM6_ESM.pdf" }, { "basename": "41467_2019_12687_MOESM2_ESM.xlsx", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM2_ESM.xlsx" }, { "basename": "41467_2019_12687_MOESM7_ESM.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM7_ESM.pdf" }, { "basename": "41467_2019_12687_MOESM8_ESM.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/41467_2019_12687_MOESM8_ESM.pdf" }, { "basename": "s41467-019-12687-4.pdf", "url": "https://authors.library.caltech.edu/records/zeg8d-38t27/files/s41467-019-12687-4.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Hockman, Dorit; Chong-Morrison, Vanessa; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cx3xe-bv582", "eprint_id": 93686, "eprint_status": "archive", "datestamp": "2023-08-22 02:24:14", "lastmod": "2023-10-23 17:03:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-Hugo-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-Robb", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" } ] }, "title": "An atlas of anterior hox gene expression in the embryonic sea lamprey head: hox-code evolution in vertebrates", "ispublished": "pub", "full_text_status": "public", "keywords": "Hox expression; Hindbrain segmentation; Cranial neural crest; Vertebrate evolution; Rhombomeres; Lamprey; Gene regulation; Axial patterning", "note": "\u00a9 2019 Elsevier Inc. \n\nReceived 26 February 2019, Revised 5 April 2019, Accepted 1 May 2019, Available online 6 May 2019. \n\nWe thank Stephen Green, Dorit Hockman, Tetsuto Miyashita, and Megan Martik for lamprey husbandry assistance, and the Stowers Institute Histology facility for sectioning assistance. HJP and RK were supported by the Stowers Institute (RK grant #2013-1001). MEB was supported by grants RO1NS108500 and R35 NS111564.\n\nAccepted Version - nihms-1529223.pdf
Submitted - 571448.full.pdf
", "abstract": "In the hindbrain and the adjacent cranial neural crest (NC) cells of jawed vertebrates (gnathostomes), nested and segmentally-restricted domains of Hox gene expression provide a combinatorial Hox-code for specifying regional properties during head development. Extant jawless vertebrates, such as the sea lamprey (Petromyzon marinus), can provide insights into the evolution and diversification of this Hox-code in vertebrates. There is evidence for gnathostome-like spatial patterns of Hox expression in lamprey; however, the expression domains of the majority of lamprey hox genes from paralogy groups (PG) 1\u20134 are yet to be characterized, so it is unknown whether they are coupled to hindbrain segments (rhombomeres) and NC. In this study, we systematically describe the spatiotemporal expression of all 14 sea lamprey hox genes from PG1-PG4 in the developing hindbrain and pharynx to investigate the extent to which their expression conforms to the archetypal gnathostome hindbrain and pharyngeal hox-codes. We find many similarities in Hox expression between lamprey and gnathostome species, particularly in rhombomeric domains during hindbrain segmentation and in the cranial neural crest, enabling inference of aspects of Hox expression in the ancestral vertebrate embryonic head. These data are consistent with the idea that a Hox regulatory network underlying hindbrain segmentation is a pan vertebrate trait. We also reveal differences in hindbrain domains at later stages, as well as expression in the endostyle and in pharyngeal arch (PA) 1 mesoderm. Our analysis suggests that many Hox expression domains that are observed in extant gnathostomes were present in ancestral vertebrates but have been partitioned differently across Hox clusters in gnathostome and cyclostome lineages after duplication.", "date": "2019-09-01", "date_type": "published", "publication": "Developmental Biology", "volume": "453", "number": "1", "publisher": "Elsevier", "pagerange": "19-33", "id_number": "CaltechAUTHORS:20190311-132253895", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190311-132253895", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Stowers Institute for Medical Research", "grant_number": "RK 2013-1001" }, { "agency": "NIH", "grant_number": "R01 NS108500" }, { "agency": "NIH", "grant_number": "R35 NS111564" } ] }, "doi": "10.1016/j.ydbio.2019.05.001", "pmcid": "PMC6667299", "primary_object": { "basename": "571448.full.pdf", "url": "https://authors.library.caltech.edu/records/cx3xe-bv582/files/571448.full.pdf" }, "related_objects": [ { "basename": "nihms-1529223.pdf", "url": "https://authors.library.caltech.edu/records/cx3xe-bv582/files/nihms-1529223.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Parker, Hugo J.; Bronner, Marianne E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/10e3e-xg115", "eprint_id": 98397, "eprint_status": "archive", "datestamp": "2023-08-19 17:28:13", "lastmod": "2023-10-18 17:19:53", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Prummel-K-D", "name": { "family": "Prummel", "given": "Karin D." } }, { "id": "Hess-C", "name": { "family": "Hess", "given": "Christopher" } }, { "id": "Nieuwenhuize-S", "name": { "family": "Nieuwenhuize", "given": "Susan" } }, { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Rogers-K-W", "name": { "family": "Rogers", "given": "Katherine W." } }, { "id": "Kozmikova-I", "name": { "family": "Kozmikova", "given": "Iryna" }, "orcid": "0000-0002-7861-9802" }, { "id": "Racioppi-C", "name": { "family": "Racioppi", "given": "Claudia" }, "orcid": "0000-0001-8117-1124" }, { "id": "Brombacher-E-C", "name": { "family": "Brombacher", "given": "Eline C." } }, { "id": "Czarkwiani-A", "name": { "family": "Czarkwiani", "given": "Anna" }, "orcid": "0000-0002-8845-3113" }, { "id": "Knapp-Dunja", "name": { "family": "Knapp", "given": "Dunja" }, "orcid": "0000-0002-7533-2208" }, { "id": "Burger-S", "name": { "family": "Burger", "given": "Sibylle" } }, { "id": "Chiavacci-E", "name": { "family": "Chiavacci", "given": "Elena" }, "orcid": "0000-0003-3924-3635" }, { "id": "Shah-Gopi", "name": { "family": "Shah", "given": "Gopi" }, "orcid": "0000-0002-0154-9455" }, { "id": "Burger-A", "name": { "family": "Burger", "given": "Alexa" }, "orcid": "0000-0001-7137-3910" }, { "id": "Huisken-J", "name": { "family": "Huisken", "given": "Jan" }, "orcid": "0000-0001-7250-3756" }, { "id": "Yun-Maximina-H", "name": { "family": "Yun", "given": "Maximina H." }, "orcid": "0000-0001-9019-2453" }, { "id": "Christiaen-L", "name": { "family": "Christiaen", "given": "Lionel" }, "orcid": "0000-0001-5930-5667" }, { "id": "Kozmik-Z", "name": { "family": "Kozmik", "given": "Zbynek" }, "orcid": "0000-0002-5850-2105" }, { "id": "M\u00fcller-P", "name": { "family": "M\u00fcller", "given": "Patrick" }, "orcid": "0000-0002-0702-6209" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-R", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" }, { "id": "Mosimann-C", "name": { "family": "Mosimann", "given": "Christian" }, "orcid": "0000-0002-0749-2576" } ] }, "title": "A conserved regulatory program initiates lateral plate mesoderm emergence across chordates", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nReceived: 8 April 2019. Accepted: 22 July 2019. Published online: 26 August 2019. \n\nWe thank Seraina B\u00f6tschi, Lukas Obernosterer, and Vesna Barros for technical and husbandry support; the lab of Stephan Neuhauss for zebrafish husbandry support; the labs of Esther St\u00f6ckli and Jerome Gros for chicken experimentation support; the ZMB at UZH for imaging support; Fiona Wardle for input and support on the ChIP-seq panel in Fig. 5a; the lab of Magdalini Polymenidou for vibratome access; Karol\u00edna Ditrychov\u00e1 for cloning the pKD001 construct; Rebecca Burdine for sharing transcription factor constructs; Mark Miller for animal illustrations in Fig. 7a; the Stowers Institute histology facility for assistance with lamprey embryo sectioning; Hans-Henning Epperlein for discussions on salamander embryology; Diego Safian for suggesting Genomicus for synteny analysis; and all members of the Mosimann lab for constructive input. \n\nThis work has been supported by a Swiss National Science Foundation (SNSF) professorship [PP00P3_139093] and SNSF R'Equip grant 150838 (Lightsheet Fluorescence Microscopy), a Marie Curie Career Integration Grant from the European Commission [CIG PCIG14-GA-2013-631984], the Canton of Z\u00fcrich, the UZH Foundation for Research in Science and the Humanities, the Swiss Heart Foundation, and the ZUNIV FAN/UZH Alumni to C.M.; a UZH CanDoc to C.H.; EuFishBioMed and Company of Biologists travel fellowships to K.D.P.; the Stowers Institute (grant #1001) to H.J.P. and R.K.; NIH/NHLBI R01 award HL108643, trans-Atlantic network of excellence award 15CVD01 from the Leducq Foundation to L.C.; a long-term fellowship ALTF 1608-2014 from EMBO to C.R.; Alexander von Humboldt fellowship to A.C. and DFG Research Center (DFG FZ 111) and Cluster of Excellence (DFG EXC 168) funds to M.H.Y.; Czech Science Foundation 17-15374\u2009S to Z.K. \n\nData availability: The authors declare that the data supporting the findings of this study are available within the paper and its supplementary information files. Original data underlying the lamprey experiments in this manuscript are accessible from the Stowers Original Data Repository at http://odr.stowers.org/websimr/. \n\nThe source data underlying Fig. 2j and Supplementary Fig. 2b and 10m are provided as a Source Data file. Reagents are available upon request. \n\nThese authors contributed equally: Karin D. Prummel, Christopher Hess. \n\nAuthor Contributions: K.D.P., C.H., S.N., E.C.B., S.B., and C.M. designed, performed, and interpreted the zebrafish experiments; S.N., E.C., and A.B. established and performed the chicken experiments; K.D.P. performed the lightsheet imaging with technical and equipment support by G.S. and J.H.; K.W.R. and P.M. provided and generated mutants and maternal-zygotic mutant zebrafish; H.J.P., M.B., and R.K. designed, performed, and interpreted the lamprey experiments; A.C., D.K., and M.H.Y. designed, performed, and interpreted the axolotl experiments; C.R. and L.C. designed, performed, and interpreted the Ciona experiments; I.K. and Z.K. designed, performed, and interpreted the amphioxus experiments; A.C., D.K., and M.H.Y. designed and performed the axolotl experiments; K.D.P., C.H., S.N., and C.M. assembled and wrote the manuscript with contributions from all co-authors. \n\nThe authors declare no competing interests. \n\nNature Communications thanks the anonymous reviewers for their contribution to the peer review of this work. Peer reviewer reports are available.\n\nPublished - s41467-019-11561-7.pdf
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", "abstract": "Cardiovascular lineages develop together with kidney, smooth muscle, and limb connective tissue progenitors from the lateral plate mesoderm (LPM). How the LPM initially emerges and how its downstream fates are molecularly interconnected remain unknown. Here, we isolate a pan-LPM enhancer in the zebrafish-specific draculin (drl) gene that provides specific LPM reporter activity from early gastrulation. In toto live imaging and lineage tracing of drl-based reporters captures the dynamic LPM emergence as lineage-restricted mesendoderm field. The drl pan-LPM enhancer responds to the transcription factors EomesoderminA, FoxH1, and MixL1 that combined with Smad activity drive LPM emergence. We uncover specific activity of zebrafish-derived drl reporters in LPM-corresponding territories of several chordates including chicken, axolotl, lamprey, Ciona, and amphioxus, revealing a universal upstream LPM program. Altogether, our work provides a mechanistic framework for LPM emergence as defined progenitor field, possibly representing an ancient mesodermal cell state that predates the primordial vertebrate embryo.", "date": "2019-08-26", "date_type": "published", "publication": "Nature Communications", "volume": "10", "publisher": "Nature Publishing Group", "pagerange": "Art. no. 3857", "id_number": "CaltechAUTHORS:20190903-130241403", "issn": "2041-1723", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190903-130241403", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Swiss National Science Foundation (SNSF)", "grant_number": "PP00P3_139093" }, { "agency": "Swiss National Science Foundation (SNSF)", "grant_number": "150838" }, { "agency": "Marie Curie Fellowship", "grant_number": "CIG PCIG14-GA-2013-631984" }, { "agency": "Canton of Z\u00fcrich" }, { "agency": "UZH Foundation for Research in Science and the Humanities" }, { "agency": "Swiss Heart Foundation" }, { "agency": "ZUNIV FAN/UZH Alumni" }, { "agency": "Universit\u00e4t Z\u00fcrich" }, { "agency": "EuFishBioMed" }, { "agency": "Company of Biologists" }, { "agency": "Stowers Institute", "grant_number": "1001" }, { "agency": "NIH", "grant_number": "HL108643" }, { "agency": "Leducq Foundation", "grant_number": "15CVD01" }, { "agency": "European Molecular Biology Organization (EMBO)", "grant_number": "ALTF 1608-2014" }, { "agency": "Alexander von Humboldt Foundation" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "DFG FZ 111" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "DFG EXC 168" }, { "agency": "Czech Science Foundation", "grant_number": "17-15374" } ] }, "doi": "10.1038/s41467-019-11561-7", "pmcid": "PMC6710290", "primary_object": { "basename": "41467_2019_11561_MOESM8_ESM.pdf", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM8_ESM.pdf" }, "related_objects": [ { "basename": "s41467-019-11561-7.pdf", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/s41467-019-11561-7.pdf" }, { "basename": "41467_2019_11561_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM1_ESM.pdf" }, { "basename": "41467_2019_11561_MOESM5_ESM.mp4", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM5_ESM.mp4" }, { "basename": "41467_2019_11561_MOESM6_ESM.pdf", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM6_ESM.pdf" }, { "basename": "41467_2019_11561_MOESM7_ESM.pdf", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM7_ESM.pdf" }, { "basename": "41467_2019_11561_MOESM2_ESM.mp4", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM2_ESM.mp4" }, { "basename": "41467_2019_11561_MOESM3_ESM.mp4", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM3_ESM.mp4" }, { "basename": "41467_2019_11561_MOESM4_ESM.mp4", "url": "https://authors.library.caltech.edu/records/10e3e-xg115/files/41467_2019_11561_MOESM4_ESM.mp4" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Prummel, Karin D.; Hess, Christopher; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/p50rd-cb024", "eprint_id": 97759, "eprint_status": "archive", "datestamp": "2023-08-19 17:16:48", "lastmod": "2023-10-18 16:41:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Martik-M-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2019, Tang et al. This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited. \n\nPublication history: Received: April 25, 2019; Accepted: August 8, 2019; Accepted Manuscript published: August 8, 2019 (version 1); Version of Record published: September 3, 2019 (version 2). \n\nWe would like to thank Drs. Xia Han and Yang Chai at University of Southern California, Center for Craniofacial Molecular Biology for being extremely supportive and kindly providing Wnt1-Cre; ZsGreenfl/fl cardiac tissue. Many thanks to Dr. Jeffrey Bush at University of California, San Francisco who generously sent us Wnt1-Cre2+; R26mTmG mouse embryos. We appreciate the help from Drs. Ann M Cavanaugh and Jau-Nian Chen at Department of Molecular, Cell and Developmental biology, University of California, Los Angeles in sharing Tg (NC: mCherry) transgenic fish line for Sox10:GAL4-UAS-Cre;ubi:Switch. We would also like to acknowledge the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory, in particular, Igor Antoshechkin for sequencing of our RNAseq libraries. We thank Rochelle Diamond and Diana Perez of the the Caltech Flow Cytometry Cell Sorting Facility for cell sorting assistance. We thank David Mayorga and Ryan Fraser of the Beckman Institute Zebrafish Facility for help with zebrafish husbandry and Joanne Tan-Cabugo and Constanza Gonzalez for technical assistance, and Beckman Institute Biological Imaging Facility for equipment. This work is supported by NIHR01DE027568 and NIHRO1HL14058 to MEB and a Helen Hay Whitney Post-doctoral Fellowship to MLM. \n\nThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. \n\nCompeting interests: Marianne E Bronner: Senior editor, eLife. The other authors declare that no competing interests exist. \n\nAuthor contributions: Weiyi Tang, Conceptualization, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing\u2014original draft, Writing\u2014review and editing, Conceived the project, performed virus preparation, lineage analysis in chick and mouse, immunohistochemistry, quantification, and wrote the manuscript; Megan L Martik, Conceptualization, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing\u2014original draft, Writing\u2014review and editing, Conceived the project, performed the heart regeneration experiments and RNAseq, and wrote the manuscript; Yuwei Li, Formal analysis, Validation, Visualization, Methodology, Writing\u2014 original draft, Writing\u2014review and editing, Performed molecular cloning for virus preparation, provided consultation for the manuscript; Marianne E Bronner, Conceptualization, Resources, Supervision, Funding acquisition, Investigation, Writing\u2014original draft, Project administration, Writing\u2014review and editing, Conceived the project, assisted with lineage analysis in chick, and wrote the manuscript. \n\nEthics: Animal experimentation: Adult zebrafish were maintained in the Beckman Institute Zebrafish Facility at Caltech, and all animal and embryo work were completed in compliance with California Institute of Technology Institutional Animal Care and Use Committee (IACUC) protocol 1764. \n\nSenior and Reviewing Editor: Didier Y Stainier, Max Planck Institute for Heart and Lung Research, Germany. \n\nData availability: All data is available in the main text, the supplementary materials. Databases have been deposited\nto NCBI (BioProject # PRJNA526570).\n\nPublished - elife-47929-v2.pdf
Accepted Version - elife-47929-v1.pdf
", "abstract": "Cardiac neural crest cells contribute to important portions of the cardiovascular system including the aorticopulmonary septum and cardiac ganglion. Using replication incompetent avian retroviruses for precise high-resolution lineage analysis, we uncover a previously undescribed neural crest contribution to cardiomyocytes of the ventricles in Gallus gallus, supported by Wnt1-Cre lineage analysis in Mus musculus. To test the intriguing possibility that neural crest cells contribute to heart repair, we examined Danio rerio adult heart regeneration in the neural crest transgenic line, Tg(-4.9sox10:eGFP). Whereas the adult heart has few sox10+ cells in the apex, sox10 and other neural crest regulatory network genes are upregulated in the regenerating myocardium after resection. The results suggest that neural crest cells contribute to many cardiovascular structures including cardiomyocytes across vertebrates and to the regenerating heart of teleost fish. Thus, understanding molecular mechanisms that control the normal development of the neural crest into cardiomyocytes and reactivation of the neural crest program upon regeneration may open potential therapeutic approaches to repair heart damage in amniotes.", "date": "2019-08-08", "date_type": "published", "publication": "eLife", "volume": "8", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e47929", "id_number": "CaltechAUTHORS:20190812-093416740", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190812-093416740", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "RO1HL14058" }, { "agency": "Helen Hay Whitney Foundation" } ] }, "local_group": { "items": [ { "id": "Millard-and-Muriel-Jacobs-Genetics-and-Genomics-Laboratory" } ] }, "doi": "10.7554/elife.47929", "pmcid": "PMC6721792", "primary_object": { "basename": "elife-47929-v1.pdf", "url": "https://authors.library.caltech.edu/records/p50rd-cb024/files/elife-47929-v1.pdf" }, "related_objects": [ { "basename": "elife-47929-v2.pdf", "url": "https://authors.library.caltech.edu/records/p50rd-cb024/files/elife-47929-v2.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Tang, Weiyi; Martik, Megan L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/z7j8w-kk591", "eprint_id": 94895, "eprint_status": "archive", "datestamp": "2023-08-22 02:07:35", "lastmod": "2023-10-20 18:22:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Diaz-R-E-Jr", "name": { "family": "Diaz", "given": "Raul E., Jr." } }, { "id": "Shylo-N-A", "name": { "family": "Shylo", "given": "Natalia A." } }, { "id": "Roellig-D", "name": { "family": "Roellig", "given": "Daniela" }, "orcid": "0000-0002-7558-3592" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Trainor-P-A", "name": { "family": "Trainor", "given": "Paul A." }, "orcid": "0000-0003-2774-3624" } ] }, "title": "Filling in the phylogenetic gaps: induction, migration and differentiation of neural crest cells in a squamate reptile, the Veiled Chameleon (Chamaeleo calyptratus)", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest cells; Squamate; Reptile; Chameleon; Evolution; Induction; Migration; HNK1; Neurulation; Cranial Nerves; Skeletogenesis", "note": "\u00a9 2019 Wiley Periodicals, Inc. \n\nAccepted manuscript online: 13 April 2019; Manuscript accepted: 04 April 2019; Manuscript revised: 04 April 2019; Manuscript received: 16 January 2019.", "abstract": "Neural crest cells comprise a migratory progenitor cell population that differentiate into cell types such as neurons and glia of the peripheral nervous system, pigment cells, hormone secreting cells in glands, and skeletal and connective tissue in the head, thus making important contributions to most tissues and organs throughout the vertebrate body. The evolutionary appearance of neural crest cells is considered synonymous with the origin of vertebrates and their subsequent diversification and radiation. While the comparative biology of neural crest cells has been studied for a century and a half beginning with their discovery by Wilhelm His in 1868 (His, 1868), most of our understanding of their development and function has come from a small number of species. Thus, critical gaps exist in our understanding of how neural crest cells mediate evolution and development. This is particularly true with respect to squamate reptiles (lizards, snakes, amphisbaenians), which account for about one\u2010third of all living tetrapods. Here we present veiled chameleons (Chamaeleo calyptratus) as a model system for studying neural crest cell development in squamates. Chameleons exhibit various morphological specializations associated with an arboreal lifestyle that may have been facilitated through neural crest cells acting as a conduit for evolutionary change.", "date": "2019-08", "date_type": "published", "publication": "Developmental Dynamics", "volume": "248", "number": "8", "publisher": "Wiley", "pagerange": "709-727", "id_number": "CaltechAUTHORS:20190423-133133773", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190423-133133773", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/dvdy.38", "resource_type": "article", "pub_year": "2019", "author_list": "Diaz, Raul E., Jr.; Shylo, Natalia A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/76jat-gv830", "eprint_id": 97138, "eprint_status": "archive", "datestamp": "2023-08-22 02:02:20", "lastmod": "2023-10-20 21:59:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Moghadasi-Boroujeni-S", "name": { "family": "Moghadasi Boroujeni", "given": "Samaneh" } }, { "id": "Koontz-A", "name": { "family": "Koontz", "given": "Alison" } }, { "id": "Tseropoulos-Georgios", "name": { "family": "Tseropoulos", "given": "Georgios" } }, { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Mehrotra-P", "name": { "family": "Mehrotra", "given": "Pihu" }, "orcid": "0000-0001-9369-5013" }, { "id": "Bajpai-Vivek-K", "name": { "family": "Bajpai", "given": "Vivek K." } }, { "id": "Selvam-S-R", "name": { "family": "Selvam", "given": "Surya Rajan" } }, { "id": "Lei-Pedro", "name": { "family": "Lei", "given": "Pedro" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Andreadis-Stelios-T", "name": { "family": "Andreadis", "given": "Stelios T." } } ] }, "title": "Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo", "ispublished": "pub", "full_text_status": "public", "keywords": "Adult stem cells; Multipotent stem cells", "note": "\u00a9 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nReceived 26 February 2019; Accepted 24 June 2019; Published 05 July 2019. \n\nThe authors declare no competing interests. \n\nThis work was supported by grants from the National Institutes of Health R01 EB023114 (S.T.A., M.E.B.) and the New York Stem Cell Science NYSTEM (Contract #C30290GG, S.T.A.). \n\nAuthor Contributions: Samaneh Moghadasi Boroujeni: Designing and performing experiments, data analysis and interpretation, writing of the manuscript. Alison Koontz, Laura Kerosuo, Marianne E. Bronner: In ovo implantation experiments design, data analysis and interpretation, writing of the manuscript; Pihu Mehrotra: Developed neuron differentiation protocol; Vivek K. Bajpai: Designing experiments, troubleshooting. Surya Rajan Selvam: Performed melanocyte and Schwann cell differentiation experiments. Georgios Tseropoulos, Pedro Lei: Keratinocyte isolation from human skin tissues. Stelios T. Andreadis: Conceived the idea, design of experiments, analysis and interpretation of data, manuscript writing and project supervision.\n\nPublished - s41598-019-46140-9.pdf
Supplemental Material - 41598_2019_46140_MOESM1_ESM.pdf
", "abstract": "Neural crest (NC) cells are multipotent stem cells that arise from the embryonic ectoderm, delaminate from the neural tube in early vertebrate development and migrate throughout the developing embryo, where they differentiate into various cell lineages. Here we show that multipotent and functional NC cells can be derived by induction with a growth factor cocktail containing FGF2 and IGF1 from cultures of human inter-follicular keratinocytes (KC) isolated from elderly donors. Adult NC cells exhibited longer doubling times as compared to neonatal NC cells, but showed limited signs of cellular senescence despite the advanced age of the donors and exhibited significantly younger epigenetic age as compared to KC. They also maintained their multipotency, as evidenced by their ability to differentiate into all NC-specific lineages including neurons, Schwann cells, melanocytes, and smooth muscle cells (SMC). Notably, upon implantation into chick embryos, adult NC cells behaved similar to their embryonic counterparts, migrated along stereotypical pathways and contributed to multiple NC derivatives in ovo. These results suggest that KC-derived NC cells may provide an easily accessible, autologous source of stem cells that can be used for treatment of neurodegenerative diseases or as a model system for studying disease pathophysiology and drug development.", "date": "2019-07-05", "date_type": "published", "publication": "Scientific Reports", "volume": "9", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 9750", "id_number": "CaltechAUTHORS:20190715-074911175", "issn": "2045-2322", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190715-074911175", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 EB023114" }, { "agency": "New York Stem Cell Science (NYSTEM)", "grant_number": "C30290GG" } ] }, "doi": "10.1038/s41598-019-46140-9", "pmcid": "PMC6611768", "primary_object": { "basename": "41598_2019_46140_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/76jat-gv830/files/41598_2019_46140_MOESM1_ESM.pdf" }, "related_objects": [ { "basename": "s41598-019-46140-9.pdf", "url": "https://authors.library.caltech.edu/records/76jat-gv830/files/s41598-019-46140-9.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Moghadasi Boroujeni, Samaneh; Koontz, Alison; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2tb09-c9087", "eprint_id": 96200, "eprint_status": "archive", "datestamp": "2023-08-19 16:14:46", "lastmod": "2023-10-20 20:58:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Soldatov-R", "name": { "family": "Soldatov", "given": "Ruslan" } }, { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Spatiotemporal structure of cell fate decisions in murine neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2019 American Association for the Advancement of Science. \n\nReceived 9 January 2018; resubmitted 12 December 2018. Accepted 10 April 2019. \n\nWe thank the Eukaryotic Single Cell Genomics Facility for the single-cell RNA-seq and the in situ sequencing pilot facility at the Science for Life Laboratory, Sweden (for technical assistance with the in situ sequencing experiments). We also thank O. Kharchenko for help with illustrations and V. Petukhov for assistance with computational analysis of in situ sequencing data. Funding: I.A. was funded by a Swedish Research Council (Vetenskapsradet, VR) grant, ERC Consolidator grant \"STEMMING-FROM-NERVE\" N647844, the Paradifference Foundation, and the Bertil Hallsten Research Foundation. P.V.K. was supported by NSF-14-532 CAREER award and NIH R01HL131768. M.N. was funded by a Swedish Research Council (Vetenskapsradet) grant 2016-03645, the Knut and Alice Wallenberg Foundation, and Familjen Erling Perssons stiftelse. V.D. was supported by a Russian Science Foundation grant (18-75-10005, immunochemistry) and the Swedish Research Council (2015-03387). M.E.K. was supported by Stiftelsen Riksbankens Jubileumsfond (Erik R\u00f6nnbergs fond stipend). N.A. was supported by RSF grant 16-15-10237. Author contributions: M.K., M.E.K., J.P., L.E., N.A., Y.Y., M.H., V.D., M.F., M.L.P., F.B., C.Y., X.Q., W.-Y.H., and L.C. acquired all biological data and performed the relevant analysis. R.S., P.V.K., and T.C. performed computational analysis of single-cell data. R.S. and P.V.K. developed computational methods. R.S. and M.M.H. performed computational analysis of in situ sequencing data. C.B., M.N., M.E.B., D.A.G., J.-F.B., G.G.C., P.E., K.F., P.V.K., and I.A. gave feedback on experimental aspects, supervised experimental approaches, and implemented the data interpretation. R.S., M.K., M.E.K., and J.P. made all figures containing data and resulting analysis (except Fig. 7). R.S., M.K., M.E.K., P.V.K., and I.A. designed the study, organized experimental work, and wrote the manuscript. All authors provided feedback on figures, manuscript composition, and structure. Competing interests: M.N. holds shares in Cartana AB, a company commercializing in situ sequencing reagents. Other authors declare no conflicts of interest. Data and materials availability: All single-cell RNA-seq datasets have been deposited in the GEO under accession code GSE129114. Processed data, code, supplementary materials, and interactive views of datasets can be accessed on the authors' website: http://pklab.med.harvard.edu/ruslan/neural.crest.html. These URLs will be maintained exactly as they are for at least 5 years with no changes. The Sox10^(CreERT2) strain is available from the laboratory of Vassilis Pachnis (The Francis Crick Institute, UK) under a material transfer agreement with the institution. All other data needed to evaluate the conclusions in the paper are present in the paper or the supplementary materials.\n\nPublished - eaas9536.full.pdf
Supplemental Material - aas9536_DataS1_to_S12.zip
Supplemental Material - aas9536_TablesS1_to_S11.zip
", "abstract": "Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single-cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial coactivation of bipotential properties followed by gradual shifts toward commitment. Competing fate programs are coactivated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.", "date": "2019-06-07", "date_type": "published", "publication": "Science", "volume": "364", "number": "6444", "publisher": "American Association for the Advancement of Science", "pagerange": "Art. No. eaas9536", "id_number": "CaltechAUTHORS:20190607-090122437", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190607-090122437", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "European Research Council (ERC)", "grant_number": "647844" }, { "agency": "Paradifference Foundation" }, { "agency": "Bertil Hallsten Research Foundation" }, { "agency": "NSF", "grant_number": "14-532" }, { "agency": "NIH", "grant_number": "R01HL131768" }, { "agency": "Swedish Research Council", "grant_number": "2016-03645" }, { "agency": "Knut and Alice Wallenberg Foundation" }, { "agency": "Familjen Erling Perssons" }, { "agency": "Russian Science Foundation", "grant_number": "18-75-10005" }, { "agency": "Swedish Research Council", "grant_number": "2015-03387" }, { "agency": "Stiftelsen Riksbankens Jubileumsfond" }, { "agency": "Russian Science Foundation", "grant_number": "16-15-10237" } ] }, "doi": "10.1126/science.aas9536", "primary_object": { "basename": "aas9536_DataS1_to_S12.zip", "url": "https://authors.library.caltech.edu/records/2tb09-c9087/files/aas9536_DataS1_to_S12.zip" }, "related_objects": [ { "basename": "aas9536_TablesS1_to_S11.zip", "url": "https://authors.library.caltech.edu/records/2tb09-c9087/files/aas9536_TablesS1_to_S11.zip" }, { "basename": "eaas9536.full.pdf", "url": "https://authors.library.caltech.edu/records/2tb09-c9087/files/eaas9536.full.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Soldatov, Ruslan; Piacentino, Michael L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4qd7d-qzy60", "eprint_id": 93862, "eprint_status": "archive", "datestamp": "2023-08-19 16:10:06", "lastmod": "2023-10-20 17:28:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Multiplex clonal analysis in the chick embryo using retrovirally-mediated combinatorial labeling", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2019 Published by Elsevier Inc. \n\nReceived 6 December 2018, Revised 11 March 2019, Accepted 12 March 2019, Available online 15 March 2019. \n\nAuthor contributions: W.T., Y.L. and M.E.B conceived the project. W.T., Y.L. and S.G performed the experiments. W.T., Y.L. and M.E.B wrote the paper with consultation from S.G. \n\nThis work is supported by NIHR01DE027568 and NIHRO1HL14058 to M.E.B and American Heart Association predoctoral fellowship 18PRE34050063 to S.G. \n\nThe authors declare no competing interests. \n\nWe thank Alison Koontz for providing insightful suggestions on probability calculation, Can Li, Felipe Vieceli, David Miller for technical support. We thank Carlos Lois laboratory and Caltech Biological Imaging Facility for sharing equipment.\n\nAccepted Version - nihms-1525644.pdf
", "abstract": "Lineage analysis plays a central role in exploring the developmental potential of stem and progenitor cell populations. In higher vertebrates, a variety of techniques have been used to label individual cells or cell populations, including interspecies grafting, intracellular microinjection, and Cre-mediated recombination. However, these approaches often suffer from difficulties in progenitor cell targeting, low cellular resolution and/or ectopic labeling. To circumvent these issues, here we utilize replication incompetent avian (RIA) retroviruses to deliver combinations of fluorescent proteins into distinct cellular compartments in chick embryos. In particular, RIA-mediated lineage tracing is optimal for long term mapping of dispersing cell populations like the neural crest. Using this tool, we confirm that trunk neural crest cells are multipotent. Furthermore, our RIA vector is engineered to be fully adaptable for other purposes such as cell fate analysis, gene perturbation studies and time-lapse imaging. Taken together, we present a novel approach of multiplex lineage analysis that can be applied to normal and perturbed development of diverse cell populations in avian embryos.", "date": "2019-06-01", "date_type": "published", "publication": "Developmental Biology", "volume": "450", "number": "1", "publisher": "Elsevier", "pagerange": "1-8", "id_number": "CaltechAUTHORS:20190315-101353338", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190315-101353338", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE027568" }, { "agency": "NIH", "grant_number": "R01HL14058" }, { "agency": "American Heart Association", "grant_number": "18PRE34050063" } ] }, "doi": "10.1016/j.ydbio.2019.03.007", "pmcid": "PMC6487888", "primary_object": { "basename": "nihms-1525644.pdf", "url": "https://authors.library.caltech.edu/records/4qd7d-qzy60/files/nihms-1525644.pdf" }, "resource_type": "article", "pub_year": "2019", "author_list": "Tang, Weiyi; Li, Yuwei; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rbpjm-3fy31", "eprint_id": 89998, "eprint_status": "archive", "datestamp": "2023-08-19 15:23:35", "lastmod": "2023-10-20 21:59:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "S\u00e1nchez-V\u00e1squez-Estefania", "name": { "family": "S\u00e1nchez-V\u00e1squez", "given": "Estefania" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Strobl-Mazzulla-Pablo-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" } ] }, "title": "Epigenetic inactivation of miR-203 as a key step in neural crest epithelial-to-mesenchymal transition", "ispublished": "pub", "full_text_status": "public", "keywords": "EMT, neural crest, miR-203, Snail2, DNA methylation, Phf12, migration", "note": "\u00a9 2019 Published by The Company of Biologists Ltd. \n\nReceived August 14, 2018; Accepted March 15, 2019; Published 25 March 2019. \n\nWe thank Dr Xinwei Cao for the two-colored sensor vector and Dr Andrew Pollok for advice on the design of sponge vector. We also thank the Wood-Whelan research fellowships and The Company of Biologists (Development Travelling Fellowship DEV-180502) for travel support for E.S.-V. \n\nThe authors declare no competing or financial interests. \n\nAuthor contributions: Conceptualization: P.H.S.-M.; Methodology: E.S.-V., P.H.S.-M.; Validation: E.S.-V.; Formal analysis: E.S.-V.; Investigation: E.S.-V., P.H.S.-M.; Resources: M.E.B., P.H.S.-M.; Writing - original draft: E.S.-V., P.H.S.-M.; Writing - review & editing: M.E.B., P.H.S.-M.; Visualization: E.S.-V., P.H.S.-M.; Supervision: M.E.B., P.H.S.-M.; Project administration: P.H.S.-M.; Funding acquisition: M.E.B., P.H.S.-M. \n\nThis work was supported by the Fogarty International Center of the National Institutes of Health (R21TW011224 to M.E.B. and P.H.S.-M.) and by the Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (PICT 2016-0747 to P.H.S.-M.). Deposited in PMC for release after 12 months.\n\nPublished - dev171017.full.pdf
Submitted - 392142.full.pdf
", "abstract": "miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3\u2032UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a 'sponge' vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.", "date": "2019-04-11", "date_type": "published", "publication": "Development", "volume": "146", "number": "7", "publisher": "Company of Biologists", "pagerange": "Art. No. dev171017", "id_number": "CaltechAUTHORS:20180927-114223566", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180927-114223566", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R21TW011224" }, { "agency": "Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica", "grant_number": "PICT 2016-0747" }, { "agency": "Wood-Whelan Research Fellowship" }, { "agency": "Company of Biologists", "grant_number": "DEV-180502" } ] }, "doi": "10.1242/dev.171017", "pmcid": "PMC6467475", "primary_object": { "basename": "392142.full.pdf", "url": "https://authors.library.caltech.edu/records/rbpjm-3fy31/files/392142.full.pdf" }, "related_objects": [ { "basename": "dev171017.full.pdf", "url": "https://authors.library.caltech.edu/records/rbpjm-3fy31/files/dev171017.full.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "S\u00e1nchez-V\u00e1squez, Estefania; Bronner, Marianne E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vh92j-f6j49", "eprint_id": 89997, "eprint_status": "archive", "datestamp": "2023-08-22 01:08:31", "lastmod": "2023-10-23 16:08:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mohlin-Sofie", "name": { "family": "Mohlin", "given": "Sofie" }, "orcid": "0000-0002-2458-3963" }, { "id": "Kunttas-Ezgi", "name": { "family": "Kunttas", "given": "Ezgi" } }, { "id": "Persson-Camilla-U", "name": { "family": "Persson", "given": "Camilla U." } }, { "id": "Abdel-Haq-Reem", "name": { "family": "Abdel-Haq", "given": "Reem" }, "orcid": "0000-0002-7418-5736" }, { "id": "Castillo-Aldo", "name": { "family": "Castillo", "given": "Aldo" } }, { "id": "Murko-Christina", "name": { "family": "Murko", "given": "Christina" }, "orcid": "0000-0003-2281-0031" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" } ] }, "title": "Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres", "ispublished": "pub", "full_text_status": "public", "keywords": "Crestospheres; Neural crest stem cells; Trunk neural crest; Stem cell maintenance; Multipotency, self-renewal", "note": "\u00a9 2019 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). \n\nReceived 17 August 2018, Revised 9 January 2019, Accepted 9 January 2019, Available online 18 January 2019. We would like to thank Elina Fredlund for technical assistance. This work was supported by the Swedish Childhood Cancer Fund, Sweden (to SM), the Mary Bev\u00e9 Foundation (to SM), Magnus Bergvall Foundation, Sweden (to SM), the Thelma Zo\u00e9ga Foundation, Sweden (to SM), Hans von Foundation (to SM), the Royal Physiographic Society in Lund, Sweden (to SM), the Gyllenstiernska Krapperup Foundation, Sweden (to SM), Gunnar Nilsson Cancer Foundation, Sweden (to SM), NIH, United States, Ruth L. Kirschstein NRSA F32HD087026 (to EK), NIH R01DE024157 (to MB), the Academy of Finland, Finland (to LK), Sigrid Jus\u00e9lius Foundation, Finland (to LK), and in part by the Division of Intramural Research of the National Institute of Dental and Craniofacial Research at the National Institutes of Health, Department of Health and Human Services.\n\nPublished - 1-s2.0-S001216061830558X-main.pdf
Submitted - 391599.full.pdf
Supplemental Material - 1-s2.0-S001216061830558X-mmc1.docx
Supplemental Material - 1-s2.0-S001216061830558X-mmc2.pdf
Supplemental Material - 1-s2.0-S001216061830558X-mmc3.pdf
", "abstract": "Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.", "date": "2019-03-15", "date_type": "published", "publication": "Developmental Biology", "volume": "447", "number": "2", "publisher": "Elsevier", "pagerange": "137-146", "id_number": "CaltechAUTHORS:20180927-114223449", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180927-114223449", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Swedish Childhood Cancer Foundation" }, { "agency": "Mary Bev\u00e9 Foundation" }, { "agency": "Magnus Bergvall's Foundation" }, { "agency": "Thelma Zo\u00e9ga Foundation" }, { "agency": "Hans von Kantzow Foundation" }, { "agency": "Royal Physiographic Society" }, { "agency": "Gyllenstiernska Krapperup Foundation" }, { "agency": "Gunnar Nilsson Cancer Foundation" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32HD087026" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "Academy of Finland" }, { "agency": "Sigrid Jus\u00e9lius Foundation" } ] }, "doi": "10.1016/j.ydbio.2019.01.010", "pmcid": "PMC6497816", "primary_object": { "basename": "1-s2.0-S001216061830558X-mmc2.pdf", "url": "https://authors.library.caltech.edu/records/vh92j-f6j49/files/1-s2.0-S001216061830558X-mmc2.pdf" }, "related_objects": [ { "basename": "1-s2.0-S001216061830558X-mmc3.pdf", "url": "https://authors.library.caltech.edu/records/vh92j-f6j49/files/1-s2.0-S001216061830558X-mmc3.pdf" }, { "basename": "391599.full.pdf", "url": "https://authors.library.caltech.edu/records/vh92j-f6j49/files/391599.full.pdf" }, { "basename": "1-s2.0-S001216061830558X-main.pdf", "url": "https://authors.library.caltech.edu/records/vh92j-f6j49/files/1-s2.0-S001216061830558X-main.pdf" }, { "basename": "1-s2.0-S001216061830558X-mmc1.docx", "url": "https://authors.library.caltech.edu/records/vh92j-f6j49/files/1-s2.0-S001216061830558X-mmc1.docx" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Mohlin, Sofie; Kunttas, Ezgi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bdaae-pew56", "eprint_id": 93763, "eprint_status": "archive", "datestamp": "2023-08-19 14:52:00", "lastmod": "2023-10-20 17:23:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-Hugo-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "De-Kumar-Bony", "name": { "family": "De Kumar", "given": "Bony" } }, { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Prummel-Karin-D", "name": { "family": "Prummel", "given": "Karin D." } }, { "id": "Hess-Christopher", "name": { "family": "Hess", "given": "Christopher" } }, { "id": "Kaufman-Charles-K", "name": { "family": "Kaufman", "given": "Charles K." } }, { "id": "Mosimann-Christian", "name": { "family": "Mosimann", "given": "Christian" }, "orcid": "0000-0002-0749-2576" }, { "id": "Wiedemann-Leanna-M", "name": { "family": "Wiedemann", "given": "Leanne M." }, "orcid": "0000-0002-0964-4676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-Robb", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" } ] }, "title": "A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nReceived 05 December 2018; Accepted 26 February 2019; Published 13 March 2019. \n\nWe thank Dorit Hockman, Tetsuto Miyashita, and Megan Martik for lamprey husbandry assistance, the Stowers Institute aquatics facility for zebrafish care, and Histology facility for sectioning assistance. This study was conducted in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the NIH and protocols approved by the Institutional Animal Care and Use Committees of the Stowers Institute (Zebrafish, RK Protocol: #2015-0149), California Institute of Technology (lamprey, MEB Protocol: #1436-11), and the veterinary office of UZH and the Canton of Z\u00fcrich. K.D.P., C.H., and C.M. were supported by Science Foundation (SNSF) professorship (C.M. grant 170623), a Marie Curie Career Integration Grant from the European Commission (C.M. grant PCIG14-GA-2013-631984), the Swiss Cancer League, and the Canton of Z\u00fcrich. H.J.P., B.D.K., L.M.W., and R.K. were supported by the Stowers Institute (R.K. grant #2013-1001). S.A.G. and M.E.B. were supported by grants R01NS086907 and R01DE017911. \n\nData availability: The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files or from the corresponding author upon reasonable request. All raw sequencing data from this study underlying Fig. 7a have been deposited in the NCBI BioProject database [https://www.ncbi.nlm.nih.gov/bioproject] under accession code PRJNA341679 and Sequence Read Archive under accession code SRP079975 and PRJNA503882. Original data underlying this manuscript can be accessed from the Stowers Original Data Repository at [http://odr.stowers.org/websimr/]. A reporting summary for this Article is available as a Supplementary Information file. \n\nAuthor Contributions: H.J.P., M.E.B. and R.K. conceived this research programme. H.J.P., B.D.K., K.D.P. and C.H. conducted the experiments. S.A.G. performed lamprey husbandry. C.K.K. and C.M. developed the crestin transgenic zebrafish line and associated constructs. H.J.P., B.D.K., C.M., L.M.W., M.E.B. and R.K. analysed the data, discussed the ideas and interpretations, and wrote the manuscript. \n\nThe authors declare no competing interests.\n\nPublished - s41467-019-09197-8.pdf
Supplemental Material - 41467_2019_9197_MOESM1_ESM.pdf
Supplemental Material - 41467_2019_9197_MOESM2_ESM.pdf
Supplemental Material - 41467_2019_9197_MOESM3_ESM.pdf
", "abstract": "In jawed vertebrates (gnathostomes), Hox genes play an important role in patterning head and jaw formation, but mechanisms coupling Hox genes to neural crest (NC) are unknown. Here we use cross-species regulatory comparisons between gnathostomes and lamprey, a jawless extant vertebrate, to investigate conserved ancestral mechanisms regulating Hox2 genes in NC. Gnathostome Hoxa2 and Hoxb2 NC enhancers mediate equivalent NC expression in lamprey and gnathostomes, revealing ancient conservation of Hox upstream regulatory components in NC. In characterizing a lamprey hox\u03b12 NC/hindbrain enhancer, we identify essential Meis, Pbx, and Hox binding sites that are functionally conserved within Hoxa2/Hoxb2 NC enhancers. This suggests that the lamprey hox\u03b12 enhancer retains ancestral activity and that Hoxa2/Hoxb2 NC enhancers are ancient paralogues, which diverged in hindbrain and NC activities. This identifies an ancestral mechanism for Hox2 NC regulation involving a Hox-TALE regulatory circuit, potentiated by inputs from Meis and Pbx proteins and Hox auto-/cross-regulatory interactions.", "date": "2019-03-13", "date_type": "published", "publication": "Nature Communications", "volume": "10", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 1189", "id_number": "CaltechAUTHORS:20190313-093703542", "issn": "2041-1723", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190313-093703542", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Swiss National Science Foundation (SNSF)", "grant_number": "170623" }, { "agency": "Marie Curie Fellowship", "grant_number": "PCIG14-GA-2013-631984" }, { "agency": "Swiss Cancer League" }, { "agency": "Canton of Z\u00fcrich" }, { "agency": "Stowers Institute for Medical Research", "grant_number": "RK 2013-1001" }, { "agency": "NIH", "grant_number": "R01NS086907" }, { "agency": "NIH", "grant_number": "R01DE017911" } ] }, "doi": "10.1038/s41467-019-09197-8", "pmcid": "PMC6416258", "primary_object": { "basename": "41467_2019_9197_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/bdaae-pew56/files/41467_2019_9197_MOESM1_ESM.pdf" }, "related_objects": [ { "basename": "41467_2019_9197_MOESM2_ESM.pdf", "url": "https://authors.library.caltech.edu/records/bdaae-pew56/files/41467_2019_9197_MOESM2_ESM.pdf" }, { "basename": "41467_2019_9197_MOESM3_ESM.pdf", "url": "https://authors.library.caltech.edu/records/bdaae-pew56/files/41467_2019_9197_MOESM3_ESM.pdf" }, { "basename": "s41467-019-09197-8.pdf", "url": "https://authors.library.caltech.edu/records/bdaae-pew56/files/s41467-019-09197-8.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Parker, Hugo J.; De Kumar, Bony; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/acdkc-c3r36", "eprint_id": 94240, "eprint_status": "archive", "datestamp": "2023-08-19 14:33:05", "lastmod": "2023-10-20 17:48:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Diaz-R-E", "name": { "family": "Diaz", "given": "R. E." } }, { "id": "Roellig-D", "name": { "family": "Roellig", "given": "D." }, "orcid": "0000-0002-7558-3592" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" }, { "id": "Trainor-P-A", "name": { "family": "Trainor", "given": "P. A." }, "orcid": "0000-0003-2774-3624" } ] }, "title": "From Climbing Trees to Phylogenetic Trees: Veiled Chameleons (Chamaeleo calyptratus) as a Squamate Model to Fill Our Evolutionary Gaps in Vertebrate Neural Crest Cell Induction, Migration and Differentiation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2019 Oxford University Press. \n\nPublished: 11 February 2019.", "abstract": "[no abstract]", "date": "2019-03", "date_type": "published", "publication": "Integrative and Comparative Biology", "volume": "59", "number": "S1", "publisher": "Oxford University Press", "pagerange": "E53", "id_number": "CaltechAUTHORS:20190328-094605963", "issn": "1540-7063", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190328-094605963", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1093/icb/icz003", "resource_type": "article", "pub_year": "2019", "author_list": "Diaz, R. E.; Roellig, D.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/395k3-vxj92", "eprint_id": 90005, "eprint_status": "archive", "datestamp": "2023-08-22 00:55:16", "lastmod": "2023-10-23 16:08:51", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Draxin alters laminin organization during basement membrane remodeling to control cranial neural crest EMT", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Laminin; Draxin; Snail2; Epithelial-to-Mesenchymal Transition", "note": "\u00a9 2018 Elsevier. \n\nReceived 31 August 2018, Revised 19 December 2018, Accepted 19 December 2018, Available online 21 December 2018. \n\nWe thank Dr. M. Simoes-Costa for providing the NC1.1 M3:EGFP construct, and Dr. M. Cheung for providing the pCIG-V5-Snail2-IRES-nls-GFP construct (Addgene plasmid # 44282). This work was supported by a National Institutes of Health grant (R01DE024157 and PO1HD037105 to M.E. Bronner), and a Ruth L. Kirschstein National Research Service Award (F32DE026355 to E.J. Hutchins). The authors declare no competing interests.\n\nAccepted Version - 1-s2.0-S001216061830589X-main_acc.pdf
Accepted Version - nihms-1517618.pdf
Submitted - 407882.full.pdf
Supplemental Material - 1-s2.0-S001216061830589X-mmc1.pdf
", "abstract": "Premigratory neural crest cells arise within the dorsal neural tube and subsequently undergo an epithelial-to-mesenchymal transition (EMT) to leave the neuroepithelium and initiate migration. Draxin is a Wnt modulator that has been shown to control the timing of cranial neural crest EMT. Here we show that this process is accompanied by three stages of remodeling of the basement membrane protein laminin, from regression to expansion and channel formation. Loss of Draxin results in blocking laminin remodeling at the regression stage, whereas ectopic maintenance of Draxin blocks remodeling at the expansion stage. The latter effect is rescued by addition of Snail2, previously shown to be downstream of Draxin. Our results demonstrate an essential function for the Wnt modulator Draxin in regulating basement membrane remodeling during cranial neural crest EMT.", "date": "2019-02-15", "date_type": "published", "publication": "Developmental Biology", "volume": "446", "number": "2", "publisher": "Elsevier", "pagerange": "151-158", "id_number": "CaltechAUTHORS:20180927-114224386", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180927-114224386", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH", "grant_number": "P01HD037105" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32DE026355" } ] }, "doi": "10.1016/j.ydbio.2018.12.021", "pmcid": "PMC6368465", "primary_object": { "basename": "1-s2.0-S001216061830589X-main_acc.pdf", "url": "https://authors.library.caltech.edu/records/395k3-vxj92/files/1-s2.0-S001216061830589X-main_acc.pdf" }, "related_objects": [ { "basename": "1-s2.0-S001216061830589X-mmc1.pdf", "url": "https://authors.library.caltech.edu/records/395k3-vxj92/files/1-s2.0-S001216061830589X-mmc1.pdf" }, { "basename": "407882.full.pdf", "url": "https://authors.library.caltech.edu/records/395k3-vxj92/files/407882.full.pdf" }, { "basename": "nihms-1517618.pdf", "url": "https://authors.library.caltech.edu/records/395k3-vxj92/files/nihms-1517618.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Hutchins, Erica J. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tn2by-qey98", "eprint_id": 92666, "eprint_status": "archive", "datestamp": "2023-08-22 00:54:30", "lastmod": "2023-10-20 15:57:53", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Vieceli-Felipe-M", "name": { "family": "Vieceli", "given": "Felipe M." }, "orcid": "0000-0001-5142-8224" }, { "id": "Gonzalez-Walter-G", "name": { "family": "Gonzalez", "given": "Walter G." }, "orcid": "0000-0003-1310-9323" }, { "id": "Li-Ang", "name": { "family": "Li", "given": "Ang" } }, { "id": "Tang-Weiyi", "name": { "family": "Tang", "given": "Weiyi" }, "orcid": "0000-0002-1279-1001" }, { "id": "Lois-C", "name": { "family": "Lois", "given": "Carlos" }, "orcid": "0000-0002-7305-2317" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "In\u00a0Vivo Quantitative Imaging Provides Insights into Trunk Neural Crest Migration", "ispublished": "pub", "full_text_status": "public", "keywords": "trunk neural crest; individual cell migration; contact-attraction; quantitative imaging", "note": "\u00a9 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). \n\nReceived 10 October 2018, Revised 29 December 2018, Accepted 10 January 2019, Available online 5 February 2019. \n\nWe thank Dr. Vikas Trivedi (EMBL) for critically reading the manuscript and the Caltech Biological Imaging Center for sharing equipment. W.G.G. was supported by the Della Martin Foundation, the American Heart Association, and the Burroughs Wellcome Fund. This project was supported by DE024157 and DE027568 (to M.E.B.).] \n\nAuthor Contributions: Y.L. and M.E.B. conceived of the project with consultation from F.M.V. Y.L. designed the experiments, performed the molecular cloning, virus preparation, live imaging, laser ablation, and cell spot and surface segmentation. F.M.V. performed viral injections and slice cultures and provided helpful suggestions on experimental design. Y.L. and F.M.V. performed the immunofluorescence. W.G.G. developed the software tools for cell-surface segmentation and performed the cell morphological analysis. A.L. performed the cell trajectory analysis. W.G.G. and A.L. performed the statistical analysis. W.T. provided help on slice cultures and improving the titers of virus. C.L. provided helpful discussion. Y.L. and M.E.B. wrote the manuscript with consultation from W.G.G, A.L., and F.M.V. \n\nThe authors declare no competing interests.\n\nPublished - 1-s2.0-S2211124719300579-main.pdf
Accepted Version - nihms-1520925.pdf
Supplemental Material - 1-s2.0-S2211124719300579-mmc1.pdf
", "abstract": "Neural crest (NC) cells undergo extensive migrations during development. Here, we couple in vivo live imaging at high resolution with custom software tools to reveal dynamic migratory behavior in chick embryos. Trunk NC cells migrate as individuals with both stochastic and biased features as they move dorsoventrally to form peripheral ganglia. Their leading edge displays a prominent fan-shaped lamellipodium that reorients upon cell-cell contact. Computational analysis reveals that when the lamellipodium of one cell touches the body of another, the two cells undergo \"contact attraction,\" often moving together and then separating via a pulling force exerted by lamellipodium. Targeted optical manipulation shows that cell interactions coupled with cell density generate a long-range biased random walk behavior, such that cells move from high to low density. In contrast to chain migration noted at other axial levels, the results show that individual trunk NC cells navigate the complex environment without tight coordination between neighbors.", "date": "2019-02-05", "date_type": "published", "publication": "Cell Reports", "volume": "26", "number": "6", "publisher": "Cell Press", "pagerange": "1489-1500", "id_number": "CaltechAUTHORS:20190205-105428612", "issn": "2211-1247", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190205-105428612", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Della Martin Foundation" }, { "agency": "American Heart Association" }, { "agency": "Burroughs Wellcome Fund" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "DE027568" } ] }, "doi": "10.1016/j.celrep.2019.01.039", "pmcid": "PMC6449054", "primary_object": { "basename": "1-s2.0-S2211124719300579-main.pdf", "url": "https://authors.library.caltech.edu/records/tn2by-qey98/files/1-s2.0-S2211124719300579-main.pdf" }, "related_objects": [ { "basename": "1-s2.0-S2211124719300579-mmc1.pdf", "url": "https://authors.library.caltech.edu/records/tn2by-qey98/files/1-s2.0-S2211124719300579-mmc1.pdf" }, { "basename": "nihms-1520925.pdf", "url": "https://authors.library.caltech.edu/records/tn2by-qey98/files/nihms-1520925.pdf" } ], "resource_type": "article", "pub_year": "2019", "author_list": "Li, Yuwei; Vieceli, Felipe M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1cz1f-wdf09", "eprint_id": 92234, "eprint_status": "archive", "datestamp": "2023-08-19 13:55:08", "lastmod": "2023-10-20 00:07:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Commentary on Le Douarin, 1973 and 1974", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2018 Published by Elsevier Inc. \n\nAvailable online 12 January 2019.", "abstract": "Developmental Biology has a long, rich history of publishing papers that have influenced the field for decades. As 2019 marks the 80th anniversary of the Society for Developmental Biology, I thought it would be useful to republish a sampling of these classic papers both to highlight their impact and also to give a sense of how the field has advanced. \n\nTo begin this series, I have chosen two papers by Nicole Le Douarin and colleagues that are personally important to me and helped to shape my thinking as a young scientist. The first is a technical note that introduces the quail-chick chimera grafting technique; the second one, published one year later, utilizes the technique to test the developmental potential of neural crest cells by grafting them to other axial levels. Published in Developmental Biology in 1973 and 1974, respectively, these papers were paradigm shifting in the neural crest field and established Le Douarin as one of the most influential scientists of the 20th century.", "date": "2019-01-15", "date_type": "published", "publication": "Developmental Biology", "volume": "445", "number": "2", "publisher": "Elsevier", "pagerange": "115-144", "id_number": "CaltechAUTHORS:20190114-072534902", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190114-072534902", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2018.12.011", "resource_type": "article", "pub_year": "2019", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/f4xkj-xe779", "eprint_id": 87590, "eprint_status": "archive", "datestamp": "2023-08-19 13:07:21", "lastmod": "2023-10-18 21:17:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Kunttas-Ezgi", "name": { "family": "Kunttas", "given": "Ezgi" } }, { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Howard-Aubrey-G-A-IV", "name": { "family": "Howard", "given": "Aubrey G. A., IV" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Uribe-R-A", "name": { "family": "Uribe", "given": "Rosa A." } } ] }, "title": "Migration and Diversification of the Vagal Neural Crest", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2018 Elsevier Inc. \n\nReceived 26 March 2018, Revised 3 July 2018, Accepted 3 July 2018, Available online 5 July 2018. \n\nWe would like to thank Dr. Max Ezin for helpful discussion, Weiyi Tang for providing images in Fig. 2, and Stephen Green for consultation on vagal neural crest. This work was supported by a Cancer Prevention & Research Institute of Texas (CPRIT) Recruitment Grant to RAU, NIH DE024157 and HD037105 to MEB, and Ruth L. Kirschstein NRSAs F32DE026355, F32HD087026 and F32HD088022 to EJH, EK and MLP, respectively. \n\nThe authors declare no competing interests.", "abstract": "Arising within the neural tube between the cranial and trunk regions of the body axis, the vagal neural crest shares interesting similarities in its migratory routes and derivatives with other neural crest populations. However, the vagal neural crest is also unique in its ability to contribute to diverse organs including the heart and enteric nervous system. This review highlights the migratory routes of the vagal neural crest and compares them across multiple vertebrates. We also summarize recent advances in understanding vagal neural crest ontogeny and discuss the contribution of this important neural crest population to the cardiovascular system and endoderm-derived organs, including the thymus, lungs and pancreas.", "date": "2018-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "444", "number": "S1", "publisher": "Elsevier", "pagerange": "S98-S109", "id_number": "CaltechAUTHORS:20180706-103152685", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180706-103152685", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Cancer Prevention and Research Institute of Texas" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32DE026355" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32HD087026" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32HD088022" } ] }, "doi": "10.1016/j.ydbio.2018.07.004", "pmcid": "PMC6320731", "resource_type": "article", "pub_year": "2018", "author_list": "Hutchins, Erica J.; Kunttas, Ezgi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/755fc-npd24", "eprint_id": 94164, "eprint_status": "archive", "datestamp": "2023-08-19 13:09:26", "lastmod": "2023-10-20 17:44:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Riding the crest for 150 years!", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2019 Published by Elsevier Inc. \n\nReceived 15 August 2018, Available online 20 March 2019.", "abstract": "Neural crest cells are a fascinating cell type, unique to vertebrates, and characterized by their migratory potential and multipotency. Here, we honor the 150th anniversary of their discovery by Professor Wilhelm His, a Swiss anatomist who made major contributions to our understanding of embryonic development. His identified the neural crest in 1868 and designated it as the Zwischenstrang or \"the cord in between,\" due to its juxtaposition between the neural tube and non-neural ectoderm. He made many important discoveries such as inventing the microtome, which made it possible to examine sections through embryos in exquisite detail.", "date": "2018-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "444", "number": "S1", "publisher": "Elsevier", "pagerange": "S1-S2", "id_number": "CaltechAUTHORS:20190327-085329922", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190327-085329922", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2019.03.001", "resource_type": "article", "pub_year": "2018", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/56s0d-z7f20", "eprint_id": 89682, "eprint_status": "archive", "datestamp": "2023-08-22 00:26:46", "lastmod": "2023-10-18 22:55:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tani-Matsuhana-Saori", "name": { "family": "Tani-Matsuhana", "given": "Saori" } }, { "id": "Vieceli-Felipe-M", "name": { "family": "Vieceli", "given": "Felipe Monteleone" }, "orcid": "0000-0001-5142-8224" }, { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Inoue-Kunio", "name": { "family": "Inoue", "given": "Kunio" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Transcriptome profiling of the cardiac neural crest reveals a critical role for MafB", "ispublished": "pub", "full_text_status": "public", "keywords": "MafB; cardiac neural crest; RNA-Seq; Sox10E2; transcription factor", "note": "\u00a9 2018 Elsevier. \n\nReceived 1 April 2018, Revised 5 September 2018, Accepted 15 September 2018, Available online 17 September 2018. \n\nWe thank Dr. Patrick Charnay for the chicken Krox20 probe and the Caltech Flow Cytometry and Genomics Facilities for technical support. This work was supported by Toyobo Biotechnology Foundation (Japan) postdoctoral fellowship and Japan Society for the Promotion of Science KAKENHI Grant Number JP16K18551 to STM, National Institutes of Health (USA) R01DE024157 and RO1HL140587 to MEB and a postdoctoral fellowship from the Shurl and Kay Curci Foundation (USA) to FMV.\n\nAccepted Version - nihms-1508445.pdf
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", "abstract": "The cardiac neural crest originates in the caudal hindbrain, migrates to the heart, and contributes to septation of the cardiac outflow tract and ventricles, an ability unique to this neural crest subpopulation. Here we have used a FoxD3 neural crest enhancer to isolate a pure population of cardiac neural crest cells for transcriptome analysis. This has led to the identification of transcription factors, signaling receptors/ligands, and cell adhesion molecules upregulated in the early migrating cardiac neural crest. We then functionally tested the role of one of the upregulated transcription factors, MafB, and found that it acts as a regulator of Sox10 expression specifically in the cardiac neural crest. Our results not only reveal the genome-wide profile of early migrating cardiac neural crest cells, but also provide molecular insight into what makes the cardiac neural crest unique.", "date": "2018-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "444", "number": "S1", "publisher": "Elsevier", "pagerange": "S209-S218", "id_number": "CaltechAUTHORS:20180917-125104544", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180917-125104544", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Toyobo Biotechnology Foundation" }, { "agency": "Japan Society for the Promotion of Science (JSPS)", "grant_number": "JP16K18551" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH", "grant_number": "R01HL140587" }, { "agency": "Shurl and Kay Curci Foundation" } ] }, "doi": "10.1016/j.ydbio.2018.09.015", "pmcid": "PMC6421117", "primary_object": { "basename": "1-s2.0-S0012160618302367-mmc6_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc6_lrg.jpg" }, "related_objects": [ { "basename": "1-s2.0-S0012160618302367-mmc7.xlsx", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc7.xlsx" }, { "basename": "1-s2.0-S0012160618302367-mmc9.xlsx", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc9.xlsx" }, { "basename": "1-s2.0-S0012160618302367-mmc1_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc1_lrg.jpg" }, { "basename": "1-s2.0-S0012160618302367-mmc3_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc3_lrg.jpg" }, { "basename": "1-s2.0-S0012160618302367-mmc4_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc4_lrg.jpg" }, { "basename": "1-s2.0-S0012160618302367-mmc5_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc5_lrg.jpg" }, { "basename": "1-s2.0-S0012160618302367-mmc2_lrg.jpg", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc2_lrg.jpg" }, { "basename": "1-s2.0-S0012160618302367-mmc8.xlsx", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/1-s2.0-S0012160618302367-mmc8.xlsx" }, { "basename": "nihms-1508445.pdf", "url": "https://authors.library.caltech.edu/records/56s0d-z7f20/files/nihms-1508445.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Tani-Matsuhana, Saori; Vieceli, Felipe Monteleone; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pcn18-z5a76", "eprint_id": 91214, "eprint_status": "archive", "datestamp": "2023-08-19 12:59:42", "lastmod": "2023-10-19 22:17:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Murko-Christina", "name": { "family": "Murko", "given": "Christina" }, "orcid": "0000-0003-2281-0031" }, { "id": "Vieceli-Felipe-Monteleone", "name": { "family": "Vieceli", "given": "Felipe Monteleone" }, "orcid": "0000-0001-5142-8224" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2018 The Authors. Published by Elsevier Inc. Under an Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License. \n\nReceived 2 October 2018, Revised 14 November 2018, Accepted 20 November 2018, Available online 27 November 2018. \n\nWe thank Diana Perez and Igor Antoshechkin for help with FACS and sequencing. This work was supported by a postdoctoral fellowship from the Curci foundation and an Erwin Schr\u00f6dinger fellowship from the Austrian science fund FWF (J3538-B19) to CM and NIH grant R01DE024157 to MEB.\n\nPublished - 1-s2.0-S2352340918315026-main.pdf
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", "abstract": "We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.", "date": "2018-12", "date_type": "published", "publication": "Data in Brief", "volume": "21", "publisher": "Elsevier", "pagerange": "2547-2553", "id_number": "CaltechAUTHORS:20181127-093947980", "issn": "2352-3409", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20181127-093947980", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Shurl and Kay Curci Foundation" }, { "agency": "FWF Der Wissenschaftsfonds", "grant_number": "J3538-B19" }, { "agency": "NIH", "grant_number": "R01DE024157" } ] }, "doi": "10.1016/j.dib.2018.11.109", "pmcid": "PMC6288396", "primary_object": { "basename": "1-s2.0-S2352340918315026-main.pdf", "url": "https://authors.library.caltech.edu/records/pcn18-z5a76/files/1-s2.0-S2352340918315026-main.pdf" }, "related_objects": [ { "basename": "1-s2.0-S2352340918315026-mmc2_lrg.jpg", "url": "https://authors.library.caltech.edu/records/pcn18-z5a76/files/1-s2.0-S2352340918315026-mmc2_lrg.jpg" }, { "basename": "1-s2.0-S2352340918315026-mmc3.docx", "url": "https://authors.library.caltech.edu/records/pcn18-z5a76/files/1-s2.0-S2352340918315026-mmc3.docx" }, { "basename": "1-s2.0-S2352340918315026-mmc4.xlsx", "url": "https://authors.library.caltech.edu/records/pcn18-z5a76/files/1-s2.0-S2352340918315026-mmc4.xlsx" }, { "basename": "1-s2.0-S2352340918315026-mmc5.xlsx", "url": "https://authors.library.caltech.edu/records/pcn18-z5a76/files/1-s2.0-S2352340918315026-mmc5.xlsx" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Murko, Christina; Vieceli, Felipe Monteleone; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/n6520-vtv30", "eprint_id": 90534, "eprint_status": "archive", "datestamp": "2023-08-19 07:46:50", "lastmod": "2023-10-18 23:30:43", "type": "monograph", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hess-C", "name": { "family": "Hess", "given": "Christopher" } }, { "id": "Prummel-K-D", "name": { "family": "Prummel", "given": "Karin D." } }, { "id": "Nieuwenhuize-S", "name": { "family": "Nieuwenhuize", "given": "Susan" } }, { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Rogers-K-W", "name": { "family": "Rogers", "given": "Katherine W." } }, { "id": "Kozmikova-I", "name": { "family": "Kozmikova", "given": "Iryna" }, "orcid": "0000-0002-7861-9802" }, { "id": "Racioppi-C", "name": { "family": "Racioppi", "given": "Claudia" }, "orcid": "0000-0001-8117-1124" }, { "id": "Burger-S", "name": { "family": "Burger", "given": "Sibylle" } }, { "id": "Brombacher-E-C", "name": { "family": "Brombacher", "given": "Eline C." } }, { "id": "Burger-A", "name": { "family": "Burger", "given": "Alexa" }, "orcid": "0000-0001-7137-3910" }, { "id": "Felker-A", "name": { "family": "Felker", "given": "Anastasia" } }, { "id": "Chiavacci-E", "name": { "family": "Chiavacci", "given": "Elena" }, "orcid": "0000-0003-3924-3635" }, { "id": "Shah-G", "name": { "family": "Shah", "given": "Gopi" } }, { "id": "Huisken-J", "name": { "family": "Huisken", "given": "Jan" }, "orcid": "0000-0001-7250-3756" }, { "id": "Kozmik-Z", "name": { "family": "Kozmik", "given": "Zbynek" }, "orcid": "0000-0002-5850-2105" }, { "id": "Christiaen-L", "name": { "family": "Christiaen", "given": "Lionel" }, "orcid": "0000-0001-5930-5667" }, { "id": "M\u00fcller-P", "name": { "family": "M\u00fcller", "given": "Patrick" }, "orcid": "0000-0002-0702-6209" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-R", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" }, { "id": "Mosimann-C", "name": { "family": "Mosimann", "given": "Christian" }, "orcid": "0000-0002-0749-2576" } ] }, "title": "A conserved regulatory program drives emergence of the lateral plate mesoderm", "ispublished": "unpub", "full_text_status": "public", "note": "The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. \n\nbioRxiv preprint first posted online Feb. 7, 2018. \n\nAuthor Contributions: C.H, K.D.P., S.N., S.B, C.M. designed, performed, and interpreted zebrafish experiments; S.N., E.C., A.B. performed chicken experiments; K. P. performed lightsheet imaging with technical and equipment support by G.S., J.H; E.C.B, K.P., A.F. performed section-based zebrafish lineage tracing; K.W.R, P.M. provided and generated mutants and maternal-zygotic mutant zebrafish; H.J.P, M.B., R.K. designed, performed, and interpreted lamprey experiments; C.R., L.C. designed, performed, and interpreted Ciona experiments; I.K, Z.K designed, performed, and interpreted amphioxus experiments; C.H., K.P., and C.M. assembled and wrote the manuscript with contributions from all co-authors. \n\nWe thank Sibylle Burger and Seraina B\u00f6tschi for technical and husbandry support; the lab of Dr. Stephan Neuhauss for zebrafish husbandry support; the labs of Dr. Esther St\u00f6ckli and Dr. Jerome Gros for chicken experimentation support; the ZBM at UZH for imaging support; Dr. Fiona Wardle for input and support on the ChIP-seq panel in Fig. 4E; the lab of Dr. Magdalini Polymenidou for vibratome access; Karol\u00edna Ditrychov\u00e1 for cloning the pKD001 construct; Dr. Ashley Bruce, Dr. Rebecca Burdine, and Dr. Michael Tsang for sharing transcription factor constructs; the Stowers Institute histology facility for assistance with lamprey embryo sectioning; and all members of the Mosimann lab for constructive input. This work has been supported by a Swiss National Science Foundation (SNSF) professorship [PP00P3_139093] and SNSF R'Equip grant 150838 (Lightsheet Fluorescence Microscopy), a Marie Curie Career Integration Grant from the European Commission [CIG PCIG14-GA-2013-631984], the Canton of Z\u00fcrich, the UZH Foundation for Research in Science and the Humanities, the Swiss Heart Foundation, and the ZUNIV FAN/UZH Alumni to C.M; a UZH CanDoc to C.H.; EuFishBioMed and Company of Biologists travel fellowships to K.D.P.; the Stowers Institute (grant #1001) to H.J.P. and R.K.; NIH/NHLBI R01 award HL108643, trans-Atlantic network of excellence award 15CVD01 from the\nLeducq Foundation to L.C.; a long-term fellowship ALTF 1608-2014 from EMBO to C.R.\n\nSubmitted - 261115.full.pdf
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Supplemental Material - 261115-3.mp4
Supplemental Material - 261115-4.mp4
", "abstract": "Cardiovascular cell lineages emerge with kidney, smooth muscle, and limb skeleton progenitors from the lateral plate mesoderm (LPM). How the LPM emerges during development and how it has evolved to form key lineages of the vertebrate body plan remain unknown. Here, we captured LPM formation by transgenic in toto imaging and lineage tracing using the first pan-LPM enhancer element from the zebrafish gene draculin (drl). drl LPM enhancer-based reporters are specifically active in LPM-corresponding territories of several chordate species, uncovering a universal LPM-specific gene program. Distinct from other mesoderm, we identified EomesA, FoxH1, and MixL1 with BMP/Nodal-controlled Smad activity as minimally required factors to drive drl-marked LPM formation. Altogether, our work provides a developmental and mechanistic framework for LPM emergence and the in vitro differentiation of cardiovascular cell types. Our findings suggest that the LPM may represent an ancient cell fate domain that predates ancestral vertebrates.", "date": "2018-11-01", "date_type": "published", "id_number": "CaltechAUTHORS:20181031-083913600", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20181031-083913600", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Swiss National Science Foundation (SNSF)", "grant_number": "PP00P3_139093" }, { "agency": "Swiss National Science Foundation (SNSF)", "grant_number": "150838" }, { "agency": "Marie Curie Fellowship", "grant_number": "CIG PCIG14-GA-2013-631984" }, { "agency": "Canton of Z\u00fcrich" }, { "agency": "UZH Foundation" }, { "agency": "Swiss Heart Foundation" }, { "agency": "University of Z\u00fcrich" }, { "agency": "EuFishBioMed" }, { "agency": "Company of Biologists" }, { "agency": "Stowers Institute", "grant_number": "1001" }, { "agency": "NIH", "grant_number": "HL108643" }, { "agency": "Leducq Foundation", "grant_number": "15CVD01" }, { "agency": "European Molecular Biology Organization (EMBO)", "grant_number": "1608-2014" } ] }, "doi": "10.1101/261115", "primary_object": { "basename": "261115-2.mp4", "url": "https://authors.library.caltech.edu/records/n6520-vtv30/files/261115-2.mp4" }, "related_objects": [ { "basename": "261115-3.mp4", "url": "https://authors.library.caltech.edu/records/n6520-vtv30/files/261115-3.mp4" }, { "basename": "261115-4.mp4", "url": "https://authors.library.caltech.edu/records/n6520-vtv30/files/261115-4.mp4" }, { "basename": "261115.full.pdf", "url": "https://authors.library.caltech.edu/records/n6520-vtv30/files/261115.full.pdf" }, { "basename": "261115-1.mp4", "url": "https://authors.library.caltech.edu/records/n6520-vtv30/files/261115-1.mp4" } ], "resource_type": "monograph", "pub_year": "2018", "author_list": "Hess, Christopher; Prummel, Karin D.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/r7r6h-e6c17", "eprint_id": 89889, "eprint_status": "archive", "datestamp": "2023-09-22 22:49:26", "lastmod": "2023-10-23 23:29:27", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Vieceli-F-M", "name": { "family": "Vieceli", "given": "Felipe Monteleone" }, "orcid": "0000-0001-5142-8224" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Leukocyte Receptor Tyrosine Kinase interacts with secreted midkine to promote survival of migrating neural crest cells", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, LTK, midkine, cell survival, apoptosis", "note": "\u00a9 2018 Published by The Company of Biologists Ltd. \n\nReceived February 6, 2018; Accepted September 13, 2018; Published 18 September 2018. \n\nWe thank Marcos Sim\u00f5es-Costa and Stephen Roberts for kindly sharing reagents, Narmada Thayapran for technical assistance, and Yuwei Li for valuable comments on the manuscript. \n\nThe authors declare no competing or financial interests. \n\nAuthor contributions: Conceptualization: F.M.V., M.E.B.; Methodology: F.M.V.; Formal analysis: F.M.V.; Investigation: F.M.V.; Data curation: F.M.V.; Writing - original draft: F.M.V., M.E.B.; Writing - review & editing: F.M.V., M.E.B.; Visualization: F.M.V.; Supervision: M.E.B.; Funding acquisition: F.M.V., M.E.B. \n\nThis work was supported by a National Institutes of Health grant (R01DE024157 to M.E.B.) and postdoctoral fellowships from the Brazilian National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico) (CNPq PDE 207656/2014-2 to F.M.V.) and the Shurl and Kay Curci Foundation (to F.M.V.). Deposited in PMC for release after 12 months. \n\nSupplementary information: Supplementary information available online at http://dev.biologists.org/lookup/doi/10.1242/dev.164046.supplemental\n\nPublished - dev164046.full.pdf
Supplemental Material - DEV164046supp.pdf
", "abstract": "Neural crest cells migrate long distances throughout the embryo and rely on extracellular signals that attract, repel and/or stimulate survival to ensure proper contribution to target derivatives. Here, we show that leukocyte receptor tyrosine kinase (LTK), an ALK-type receptor tyrosine kinase, is expressed by neural crest cells during early migratory stages in chicken embryos. Loss of LTK in the cranial neural crest impairs migration and results in increased levels of apoptosis. Conversely, midkine, previously proposed as a ligand for ALK, is secreted by the non-neural ectoderm during early neural crest migratory stages and internalized by neural crest cells in vivo. Similar to loss of LTK, loss of midkine reduces survival of the migratory neural crest. Moreover, we show by proximity ligation and co-immunoprecipitation assays that midkine binds to LTK. Taken together, these results suggest that LTK in neural crest cells interacts with midkine emanating from the non-neural ectoderm to promote cell survival, revealing a new signaling pathway that is essential for neural crest development.", "date": "2018-10-24", "date_type": "published", "publication": "Development", "volume": "145", "number": "20", "publisher": "Company of Biologists", "pagerange": "Art. No. dev164046", "id_number": "CaltechAUTHORS:20180924-132214153", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180924-132214153", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)", "grant_number": "PDE 207656/2014-2" }, { "agency": "Surl and Kay Curci Foundation" } ] }, "doi": "10.1242/dev.164046", "pmcid": "PMC6215395", "primary_object": { "basename": "dev164046.full.pdf", "url": "https://authors.library.caltech.edu/records/r7r6h-e6c17/files/dev164046.full.pdf" }, "related_objects": [ { "basename": "DEV164046supp.pdf", "url": "https://authors.library.caltech.edu/records/r7r6h-e6c17/files/DEV164046supp.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Vieceli, Felipe Monteleone and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/a9gc0-96n36", "eprint_id": 88116, "eprint_status": "archive", "datestamp": "2023-08-19 11:39:35", "lastmod": "2023-10-18 21:48:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hutchins-Erica-J", "name": { "family": "Hutchins", "given": "Erica J." }, "orcid": "0000-0002-4316-0333" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Draxin acts as a molecular rheostat of canonical Wnt signaling to control cranial neural crest EMT", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2018 Hutchins and Bronner. This article is distributed under the terms of an Attribution\u2013Noncommercial\u2013Share Alike\u2013No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution\u2013Noncommercial\u2013Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). \n\nSubmitted: 28 September 2017; Revision received 24 May 2018; Accepted: 5 July 2018. \n\nWe thank Dr. M. Sim\u00f5es-Costa (Cornell University, Ithaca, NY) for providing the NC1.1M3:EGFP construct, Dr. X. He (Boston Children's Hospital, Harvard Medical School, Boston, MA) for providing the human LRP5 plasmid, and Dr. S. Chapman (Clemson University, Clemson, SC) for providing the chick Dkk-1 probe template. We also thank Dr. M. Piacentino and S. Wilbert for technical assistance, Shashank Gandhi and Dr. Ruth Williams for helpful CRISPR suggestions, Drs. M. Martik, R. Uribe, and M. Piacentino for critical manuscript comments, and M. Maline for illustrations. \n\nThis work was supported by a National Institutes of Health grant (R01DE024157 to M.E. Bronner) and a Ruth L. Kirschstein National Research Service Award (F32DE026355 to E.J. Hutchins). \n\nThe authors declare no competing financial interests. \n\nAuthor contributions: E.J. Hutchins and M.E. Bronner conceived and designed the experimental approach. E.J. Hutchins performed the experiments and analyzed the data. E.J. Hutchins and M.E. Bronner wrote the manuscript.\n\nPublished - 3683.full-text.pdf
Supplemental Material - jcb.201709149.supp.pdf
", "abstract": "Neural crest cells undergo a spatiotemporally regulated epithelial-to-mesenchymal transition (EMT) that proceeds head to tailward to exit from the neural tube. In this study, we show that the secreted molecule Draxin is expressed in a transient rostrocaudal wave that mirrors this emigration pattern, initiating after neural crest specification and being down-regulated just before delamination. Functional experiments reveal that Draxin regulates the timing of cranial neural crest EMT by transiently inhibiting canonical Wnt signaling. Ectopic maintenance of Draxin in the cranial neural tube blocks full EMT; while cells delaminate, they fail to become mesenchymal and migratory. Loss of Draxin results in premature delamination but also in failure to mesenchymalize. These results suggest that a pulse of intermediate Wnt signaling triggers EMT and is necessary for its completion. Taken together, these data show that transient secreted Draxin mediates proper levels of canonical Wnt signaling required to regulate the precise timing of initiation and completion of cranial neural crest EMT.", "date": "2018-10", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "217", "number": "10", "publisher": "Rockefeller University Press", "pagerange": "3863-3697", "id_number": "CaltechAUTHORS:20180723-090943347", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180723-090943347", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32DE026355" } ] }, "doi": "10.1083/jcb.201709149", "pmcid": "PMC6168252", "primary_object": { "basename": "3683.full-text.pdf", "url": "https://authors.library.caltech.edu/records/a9gc0-96n36/files/3683.full-text.pdf" }, "related_objects": [ { "basename": "jcb.201709149.supp.pdf", "url": "https://authors.library.caltech.edu/records/a9gc0-96n36/files/jcb.201709149.supp.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Hutchins, Erica J. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wvh20-3te47", "eprint_id": 87944, "eprint_status": "archive", "datestamp": "2023-08-21 23:46:20", "lastmod": "2023-10-18 21:33:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rogers-Crystal-D", "name": { "family": "Rogers", "given": "Crystal D." }, "orcid": "0000-0002-9549-1089" }, { "id": "Sorrells-Lisa-K", "name": { "family": "Sorrells", "given": "Lisa K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices", "ispublished": "pub", "full_text_status": "public", "keywords": "E-cadherin; N-cadherin; Neural crest; Neuroectoderm; Specification; Proliferation; Cell fate; \u03b2-Catenin", "note": "\u00a9 2018 Published by Elsevier B.V. \n\nReceived 12 April 2018, Revised 16 June 2018, Accepted 2 July 2018, Available online 14 July 2018. \n\nWe would like to thank Dr. Mary-Pat Stein at CSUN and the Bronner lab at Caltech for helpful discussions, the CSUN biology department for funding and assistance, and the NIH NICHD for funding. Special thanks to Jackie Phillips and Karla Terrazas for their assistance with this project and for being super students and super women. \n\nNo competing interests declared. \n\nAuthor contributions: Conceptualization, CDR and MEB; Methodology, CDR; Investigation, CDR and LKS; Validation and Formal Analysis, CDR; Writing \u2013Original Draft, CDR; Writing \u2013Review & Editing, CDR and MEB; Visualization, CDR; Funding Acquisition, CDR and MEB; Resources, CDR and MEB; Supervision, CDR and MEB. \n\nThis work was supported by a National Institute of Health, NICHD P01 grant HD037105 to MEB, Startup Funding from CSUN to CDR, and a National Institute of Health, NICHD R15 grant R15HD092170 to CDR.\n\nAccepted Version - nihms-1500927.pdf
Supplemental Material - 1-s2.0-S0925477318300819-mmc1.pdf
Supplemental Material - 1-s2.0-S0925477318300819-mmc2.docx
", "abstract": "Characterizing endogenous protein expression, interaction and function, this study identifies in vivo interactions and competitive balance between N-cadherin and E-cadherin in developing avian (Gallus gallus) neural and neural crest cells. Numerous cadherin proteins, including neural cadherin (Ncad) and epithelial cadherin (Ecad), are expressed in the developing neural plate as well as in neural crest cells as they delaminate from the newly closed neural tube. To clarify independent or coordinate function during development, we examined their expression in the cranial region. The results revealed surprising overlap and distinct localization of Ecad and Ncad in the neural tube. Using a proximity ligation assay and co-immunoprecipitation, we found that Ncad and Ecad formed heterotypic complexes in the developing neural tube, and that modulation of Ncad levels led to reciprocal gain or reduction of Ecad protein, which then alters ectodermal cell fate. Here, we demonstrate that the balance of Ecad and Ncad is dependent upon the availability of \u03b2-catenin proteins, and that alteration of either classical cadherin modifies the proportions of the neural crest and neuroectodermal cells that are specified.", "date": "2018-08", "date_type": "published", "publication": "Mechanisms of Development", "volume": "152", "publisher": "Elsevier", "pagerange": "44-56", "id_number": "CaltechAUTHORS:20180717-154246397", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180717-154246397", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California State University, Northridge" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "R15HD092170" } ] }, "doi": "10.1016/j.mod.2018.07.003", "pmcid": "PMC6112866", "primary_object": { "basename": "1-s2.0-S0925477318300819-mmc2.docx", "url": "https://authors.library.caltech.edu/records/wvh20-3te47/files/1-s2.0-S0925477318300819-mmc2.docx" }, "related_objects": [ { "basename": "nihms-1500927.pdf", "url": "https://authors.library.caltech.edu/records/wvh20-3te47/files/nihms-1500927.pdf" }, { "basename": "1-s2.0-S0925477318300819-mmc1.pdf", "url": "https://authors.library.caltech.edu/records/wvh20-3te47/files/1-s2.0-S0925477318300819-mmc1.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Rogers, Crystal D.; Sorrells, Lisa K.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xxw21-nf803", "eprint_id": 87968, "eprint_status": "archive", "datestamp": "2023-08-19 10:38:25", "lastmod": "2023-10-18 21:34:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Neppala-Pushpa", "name": { "family": "Neppala", "given": "Pushpa" } }, { "id": "Hsin-Jenny", "name": { "family": "Hsin", "given": "Jenny" } }, { "id": "Mohlin-Sofie", "name": { "family": "Mohlin", "given": "Sofie" }, "orcid": "0000-0002-2458-3963" }, { "id": "Vieceli-Felipe-Monteleone", "name": { "family": "Vieceli", "given": "Felipe Monteleone" }, "orcid": "0000-0001-5142-8224" }, { "id": "T\u00f6r\u00f6k-Zsofia", "name": { "family": "T\u00f6r\u00f6k", "given": "Zsofia" } }, { "id": "Laine-Anni", "name": { "family": "Laine", "given": "Anni" } }, { "id": "Westermarck-Jukka", "name": { "family": "Westermarck", "given": "Jukka" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Enhanced expression of MycN/CIP2A drives neural crest toward a neural stem cell-like fate: Implications for priming of neuroblastoma", "ispublished": "pub", "full_text_status": "public", "keywords": "neuroblastoma initiation | MycN | CIP2A | neural crest | Sox2", "note": "\u00a9 2018 The Author(s). Published under the PNAS license. \n\nContributed by Marianne E. Bronner, June 13, 2018 (sent for review January 3, 2018; reviewed by Angela Nieto and Carol Thiele-Galetto) ; published ahead of print July 18, 2018. https://doi.org/10.1073/pnas.1800039115 \n\nWe thank Dr. Marie Arsenian-Henriksson for providing SK-N-BE(2) cells, Dr. Kristina Cole for providing NGP cells, Dr. Ruth Palmer for providing SK-N-AS cells, and Dr. Edward K. Chan for the mouse monoclonal CIP2A antibody. This work was funded by NIH Grants HD037105 and DE024157 (to M.E.B.) and by grants from the Jane and Aatos Erkko Foundation, the Ella and Georg Ehrnrooth Foundation, and the V\u00e4re Foundation (to L.K.), the American-Scandinavian Foundation (to P.N.), and the Sigrid Juselius Foundation (to J.W.). \n\nAuthor contributions: L.K., P.N., S.M., J.W., and M.E.B. designed research; L.K., P.N., J.H., F.M.V., Z.T., and A.L. performed research; L.K. and S.M. contributed new reagents/analytic tools; L.K., P.N., J.H., S.M., and F.M.V. analyzed data; and L.K., S.M., J.W., and M.E.B. wrote the paper. \n\nReviewers: A.N., Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Cient\u00edficas-Universidad Miguel Hern\u00e1ndez; and C.T.-G., National Institutes of Health. \n\nThe authors declare no conflict of interest. \n\nThis article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1800039115/-/DCSupplemental.\n\nPublished - E7351.full.pdf
Supplemental Material - pnas.1800039115.sapp.pdf
", "abstract": "Neuroblastoma is a neural crest-derived childhood tumor of the peripheral nervous system in which MycN amplification is a hallmark of poor prognosis. Here we show that MycN is expressed together with phosphorylation-stabilizing factor CIP2A in regions of the neural plate destined to form the CNS, but MycN is excluded from the neighboring neural crest stem cell domain. Interestingly, ectopic expression of MycN or CIP2A in the neural crest domain biases cells toward CNS-like neural stem cells that express Sox2. Consistent with this, some forms of neuroblastoma have been shown to share transcriptional resemblance with CNS neural stem cells. As high MycN/CIP2A levels correlate with poor prognosis, we posit that a MycN/CIP2A-mediated cell-fate bias may reflect a possible mechanism underlying early priming of some aggressive forms of neuroblastoma. In contrast to MycN, its paralogue cMyc is normally expressed in the neural crest stem cell domain and typically is associated with better overall survival in clinical neuroblastoma, perhaps reflecting a more \"normal\" neural crest-like state. These data suggest that priming for some forms of aggressive neuroblastoma may occur before neural crest emigration from the CNS and well before sympathoadrenal specification.", "date": "2018-07-31", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "115", "number": "31", "publisher": "National Academy of Sciences", "pagerange": "E7351-E7360", "id_number": "CaltechAUTHORS:20180718-144644668", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180718-144644668", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "Jane and Aatos Erkko Foundation" }, { "agency": "Ella and Georg Ehrnrooth Foundation" }, { "agency": "V\u00e4re Foundation" }, { "agency": "American-Scandinavian Foundation" }, { "agency": "Sigrid Juselius Foundation" } ] }, "doi": "10.1073/pnas.1800039115", "pmcid": "PMC6077707", "primary_object": { "basename": "E7351.full.pdf", "url": "https://authors.library.caltech.edu/records/xxw21-nf803/files/E7351.full.pdf" }, "related_objects": [ { "basename": "pnas.1800039115.sapp.pdf", "url": "https://authors.library.caltech.edu/records/xxw21-nf803/files/pnas.1800039115.sapp.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Kerosuo, Laura; Neppala, Pushpa; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0cwb2-7rh58", "eprint_id": 87504, "eprint_status": "archive", "datestamp": "2023-08-19 09:31:07", "lastmod": "2023-10-20 21:51:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Piacentino-M-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2018 Piacentino, Bronner. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived: October 4, 2017; Accepted: June 13, 2018; Published: June 27, 2018. \n\nData Availability Statement: All relevant experimental data are within the paper, and underlying data can be found in S1 Data. Chicken CKIP-1 (Accession #KY982274) and Smurf1 (Accession #KY982275) cDNA sequences have been uploaded to Genbank. \n\nFunding: Eunice Kennedy Shriver National Institute of Child Health and Human Development https://www.nih.gov/ (grant number F32 HD088022). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Eunice Kennedy Shriver National Institute of Child Health and Human Development https://www.nih.gov/ (grant number P01 HD037105). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Institute of Dental and Craniofacial Research https://www.nih.gov/ (grant number R01 DE024157). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. \n\nWe would like to acknowledge Elisa Mart\u00ed, Anna-Katerina Hadjantonakis, and Gerald Thomsen for sharing reagents. We also thank Steven Wilbert, Johanna Tan-Cabugao, and Harry Choi for technical assistance and Erica Hutchins, Stephen Green, and Megan Martik for valuable discussion. Confocal imaging was performed in the Biological Imaging Facility with the support of the Caltech Beckman Institute and the Arnold and Mabel Beckman Foundation. \n\nAuthor Contributions: \nConceptualization: Michael L. Piacentino, Marianne E. Bronner.\nData curation: Michael L. Piacentino.\nFormal analysis: Michael L. Piacentino.\nFunding acquisition: Michael L. Piacentino, Marianne E. Bronner.\nInvestigation: Michael L. Piacentino.\nMethodology: Michael L. Piacentino.\nResources: Michael L. Piacentino.\nSupervision: Marianne E. Bronner.\nVisualization: Michael L. Piacentino.\nWriting \u00b1 original draft: Michael L. Piacentino, Marianne E. Bronner.\nWriting \u00b1 review & editing: Michael L. Piacentino, Marianne E. Bronner. \n\nThe authors have declared that no competing interests exist.\n\nPublished - journal.pbio.2004425.pdf
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Supplemental Material - journal.pbio.2004425.s006.xlsx
Supplemental Material - journal.pbio.2004425.s007.xlsx
", "abstract": "The neural crest is induced at the neural plate border during gastrulation by combined bone morphogenetic protein (BMP), fibroblast growth factor (FGF), and Wnt signaling. While intermediate BMP levels are critical for this induction, secreted BMP inhibitors are largely absent from the neural plate border. Here, we propose a morphogen model in which intracellular attenuation of BMP signaling sets the required intermediate levels to maintain neural crest induction. We show that the scaffold protein casein kinase interacting protein 1 (CKIP-1) and ubiquitin ligase Smad ubiquitin regulatory factor 1 (Smurf1) are coexpressed with BMP4 at the chick neural plate border. Knockdown of CKIP-1 during a critical period between gastrulation and neurulation causes neural crest loss. Consistent with specific BMP modulation, CKIP-1 loss suppresses phospho-Smads 1/5/8 (pSmad1/5/8) and BMP reporter output but has no effect on Wnt signaling; Smurf1 overexpression (OE) acts similarly. Epistasis experiments further show that CKIP-1 rescues Smurf1-mediated neural crest loss. The results support a model in which CKIP-1 suppresses Smurf1-mediated degradation of Smads, uncovering an intracellular mechanism for attenuation of BMP signaling to the intermediate levels required for maintenance of neural crest induction.", "date": "2018-06", "date_type": "published", "publication": "PLoS Biology", "volume": "16", "number": "6", "publisher": "Public Library of Science", "pagerange": "Art. No. e2004425", "id_number": "CaltechAUTHORS:20180702-081840117", "issn": "1544-9173", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180702-081840117", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32 HD088022" }, { "agency": "NIH", "grant_number": "P01 HD037105" }, { "agency": "NIH", "grant_number": "R01 DE024157" }, { "agency": "Caltech Beckman Institute" }, { "agency": "Arnold and Mabel Beckman Foundation" } ] }, "doi": "10.1371/journal.pbio.2004425", "pmcid": "PMC6039030", "primary_object": { "basename": "journal.pbio.2004425.s001.tif", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s001.tif" }, "related_objects": [ { "basename": "journal.pbio.2004425.s002.tif", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s002.tif" }, { "basename": "journal.pbio.2004425.s003.tif", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s003.tif" }, { "basename": "journal.pbio.2004425.s004.tif", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s004.tif" }, { "basename": "journal.pbio.2004425.s005.tif", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s005.tif" }, { "basename": "journal.pbio.2004425.s006.xlsx", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s006.xlsx" }, { "basename": "journal.pbio.2004425.s007.xlsx", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.s007.xlsx" }, { "basename": "journal.pbio.2004425.pdf", "url": "https://authors.library.caltech.edu/records/0cwb2-7rh58/files/journal.pbio.2004425.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Piacentino, Michael L. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b5nfc-vh963", "eprint_id": 87551, "eprint_status": "archive", "datestamp": "2023-08-19 09:31:31", "lastmod": "2023-10-18 21:15:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rajan-Sriivatsan-G", "name": { "family": "Rajan", "given": "Sriivatsan G." } }, { "id": "Gallik-Kristin-L", "name": { "family": "Gallik", "given": "Kristin L." } }, { "id": "Monaghan-James-R", "name": { "family": "Monaghan", "given": "James R." } }, { "id": "Uribe-Rosa-A", "name": { "family": "Uribe", "given": "Rosa A." }, "orcid": "0000-0002-0427-4493" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" } ] }, "title": "Tracking neural crest cell cycle progression in vivo", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2018 Wiley Periodicals, Inc. \n\nIssue Online: 08 August 2018; Version of Record online: 28 June 2018; Manuscript accepted: 26 April 2018; Manuscript revised: 23 April 2018; Manuscript received: 18 February 2018. \n\nFunding Information: \nNIH. Grant Numbers: R01\u2010DE024157. \nChicago Biomedical Consortium with support from the Searle Funds at The Chicago Community Trust.\n\nAccepted Version - nihms964581.pdf
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Supplemental Material - dvg23214-sup-0006-suppinfo1.docx
", "abstract": "Analysis of cell cycle entry/exit and progression can provide fundamental insights into stem cell propagation, maintenance, and differentiation. The neural crest is a unique stem cell population in vertebrate embryos that undergoes long\u2010distance collective migration and differentiation into a wide variety of derivatives. Using traditional techniques such as immunohistochemistry to track cell cycle changes in such a dynamic population is challenging, as static time points provide an incomplete spatiotemporal picture. In contrast, the fluorescent, ubiquitination\u2010based cell cycle indicator (Fucci) system provides in vivo readouts of cell cycle progression and has been previously adapted for use in zebrafish. The most commonly used Fucci systems are ubiquitously expressed, making tracking of a specific cell population challenging. Therefore, we generated a transgenic zebrafish line, Tg(\u20104.9sox10:mAG\u2010gmnn(1/100)\u20102A\u2010mCherry\u2010cdt1(1/190)), in which the Fucci system is specifically expressed in delaminating and migrating neural crest cells. Here, we demonstrate validation of this new tool and its use in live high\u2010resolution tracking of cell cycle progression in the neural crest and derivative populations.", "date": "2018-06", "date_type": "published", "publication": "Genesis", "volume": "56", "number": "6-7", "publisher": "Wiley", "pagerange": "Art. No. e23214", "id_number": "CaltechAUTHORS:20180705-123554923", "issn": "1526-954X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180705-123554923", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Searle Scholars Program" }, { "agency": "NIH", "grant_number": "R01-DE024157" } ] }, "doi": "10.1002/dvg.23214", "pmcid": "PMC6143351", "primary_object": { "basename": "dvg23214-sup-0003-suppinfofs3.tif", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0003-suppinfofs3.tif" }, "related_objects": [ { "basename": "dvg23214-sup-0004-suppinfomov1.mov", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0004-suppinfomov1.mov" }, { "basename": "dvg23214-sup-0005-suppinfomov2.mov", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0005-suppinfomov2.mov" }, { "basename": "dvg23214-sup-0006-suppinfo1.docx", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0006-suppinfo1.docx" }, { "basename": "nihms964581.pdf", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/nihms964581.pdf" }, { "basename": "dvg23214-sup-0001-suppinfofs1.tif", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0001-suppinfofs1.tif" }, { "basename": "dvg23214-sup-0002-suppinfofs2.tif", "url": "https://authors.library.caltech.edu/records/b5nfc-vh963/files/dvg23214-sup-0002-suppinfofs2.tif" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Rajan, Sriivatsan G.; Gallik, Kristin L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rt20n-mff72", "eprint_id": 84637, "eprint_status": "archive", "datestamp": "2023-08-21 22:45:23", "lastmod": "2023-10-18 16:25:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nandagopal-Nagarajan", "name": { "family": "Nandagopal", "given": "Nagarajan" } }, { "id": "Santat-Leah-A", "name": { "family": "Santat", "given": "Leah A." } }, { "id": "LeBon-Lauren", "name": { "family": "LeBon", "given": "Lauren" } }, { "id": "Sprinzak-David", "name": { "family": "Sprinzak", "given": "David" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Elowitz-M-B", "name": { "family": "Elowitz", "given": "Michael B." }, "orcid": "0000-0002-1221-0967" } ] }, "title": "Dynamic Ligand Discrimination in the Notch Signaling Pathway", "ispublished": "pub", "full_text_status": "public", "keywords": "systems biology; Notch pathway; intercellular signaling; ligand multiplicity; single cell dynamics; signaling dynamics; signal encoding; signal decoding; myogenesis", "note": "\u00a9 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). \n\nOpen Access funded by the US Department of Defense (DoD) or performed by an employee of DoD. \n\nAvailable online 1 February 2018. \n\nAuthor Contributions: Conceptualization, N.N. and M.B.E.; Methodology, N.N. and M.B.E.; Investigation, N.N. and L.A.S.; Resources, L.L.B. and M.E.B.; Writing \u2013 Original Draft, N.N. and M.B.E.; Writing \u2013 Review & Editing, N.N., L.A.S., D.S., M.E.B., and M.B.E.; Visualization, N.N., D.S., an d M.B.E.; Supervision and Funding Acquisition, M.B.E. \n\nWe thank Mark Budde, Joe Markson, Pulin Li, Yihan Lin, James Linton, Emily Capra, Jordi Garcia-Ojalvo, and Xiaojing Gao for critical feedback on the manuscript, and Young-Wook Jun, Roy Kishony, Irv Bernstein, Stephen Blacklow, and Elizabeth Jensen for helpful discussions. Harry Choi and Colby Calvert, Caltech Flow Cytometry Facility, Caltech Biological Imaging Facility, and the Millard and Muriel Jacobs Genetics and Genomics Laboratory at Caltech provided essential technical assistance. This work was supported by the Defense Advanced Research Projects Agency (HR0011-16-0138), by the NIH (R01 HD075335), and the NSF (EFRI 1137269). N.N. was a Howard Hughes Medical Institute International Student Research fellow. \n\nThe authors declare no competing interests.\n\nPublished - 1-s2.0-S0092867418300023-main.pdf
Accepted Version - nihms-999667.pdf
Supplemental Material - mmc1.pdf
Supplemental Material - mmc2.mp4
Supplemental Material - mmc3.mp4
Supplemental Material - mmc4.mp4
Supplemental Material - mmc5.mp4
Supplemental Material - mmc6.mp4
", "abstract": "The Notch signaling pathway comprises multiple ligands that are used in distinct biological contexts. In principle, different ligands could activate distinct target programs in signal-receiving cells, but it is unclear how such ligand discrimination could occur. Here, we show that cells use dynamics to discriminate signaling by the ligands Dll1 and Dll4 through the Notch1 receptor. Quantitative single-cell imaging revealed that Dll1 activates Notch1 in discrete, frequency-modulated pulses that specifically upregulate the Notch target gene Hes1. By contrast, Dll4 activates Notch1 in a sustained, amplitude-modulated manner that predominantly upregulates Hey1 and HeyL. Ectopic expression of Dll1 or Dll4 in chick neural crest produced opposite effects on myogenic differentiation, showing that ligand discrimination can occur in vivo. Finally, analysis of chimeric ligands suggests that ligand-receptor clustering underlies dynamic encoding of ligand identity. The ability of the pathway to utilize ligands as distinct communication channels has implications for diverse Notch-dependent processes.", "date": "2018-02-08", "date_type": "published", "publication": "Cell", "volume": "172", "number": "4", "publisher": "Cell Press", "pagerange": "869-880", "id_number": "CaltechAUTHORS:20180201-152120268", "issn": "0092-8674", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180201-152120268", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Defense Advanced Research Projects Agency (DARPA)", "grant_number": "HR0011-16-0138" }, { "agency": "NIH", "grant_number": "R01 HD075335" }, { "agency": "NSF", "grant_number": "EFMA-1137269" }, { "agency": "Howard Hughes Medical Institute (HHMI)" } ] }, "doi": "10.1016/j.cell.2018.01.002", "pmcid": "PMC6414217", "primary_object": { "basename": "mmc4.mp4", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc4.mp4" }, "related_objects": [ { "basename": "mmc5.mp4", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc5.mp4" }, { "basename": "mmc6.mp4", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc6.mp4" }, { "basename": "nihms-999667.pdf", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/nihms-999667.pdf" }, { "basename": "1-s2.0-S0092867418300023-main.pdf", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/1-s2.0-S0092867418300023-main.pdf" }, { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc1.pdf" }, { "basename": "mmc2.mp4", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc2.mp4" }, { "basename": "mmc3.mp4", "url": "https://authors.library.caltech.edu/records/rt20n-mff72/files/mmc3.mp4" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Nandagopal, Nagarajan; Santat, Leah A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cd2pp-drm03", "eprint_id": 82972, "eprint_status": "archive", "datestamp": "2023-08-21 22:33:14", "lastmod": "2023-10-17 22:48:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uribe-Rosa-A", "name": { "family": "Uribe", "given": "Rosa A." }, "orcid": "0000-0002-0427-4493" }, { "id": "Hong-Stephanie-S", "name": { "family": "Hong", "given": "Stephanie S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells", "ispublished": "pub", "full_text_status": "public", "keywords": "Retinoic Acid; neural crest; meis3; zebrafish; enteric nervous system", "note": "\u00a9 2017 Elsevier Inc. \n\nReceived 10 October 2017, Accepted 23 October 2017, Available online 3 November 2017. \n\nWe thank Kenneth Poss, Stephanie Woo and Iain Shepherd for fish lines, the Zebrafish International Resource Center (ZIRC) for cDNA constructs, David Tobin for the pME-Turquoise construct. We thank Yuwei Li for help with cell track analysis, Can Li, Wael El-Nachef, Kendrick Shen and Joanne Tan-Cabugao for technical assistance and David Mayorga for fish care. Confocal imaging and Imaris image analysis for this study was performed in the Biological Imaging Facility (BIF), Caltech. This work was funded by a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award (PDEP) and a NIH National Research Service Award (NRSA) HD080343 to R.A.U.; by a Caltech Summer Undergraduate Research Fellowship (SURF) to S.S.H; by a NIH grant DE024157 and a fish facility grant from Beckman Institute, Caltech, to M.E.B. The authors declare no competing financial interests. \n\nAuthor Contributions: R.A.U. and M.E.B designed the study; R.A.U. and S.S.H. performed experiments and collected data; R.A.U. and M.E.B. analyzed data, drafted the manuscript and obtained funding.\n\nAccepted Version - nihms919756.pdf
Supplemental Material - FigS1.jpg
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", "abstract": "The enteric nervous system arises from neural crest cells that migrate as chains into and along the primitive gut, subsequently differentiating into enteric neurons and glia. Little is known about the mechanisms governing neural crest migration en route to and along the gut in vivo. Here, we report that Retinoic Acid (RA) temporally controls zebrafish enteric neural crest cell chain migration. In vivo imaging reveals that RA loss severely compromises the integrity and migration of the chain of neural crest cells during the window of time window when they are moving along the foregut. After loss of RA, enteric progenitors accumulate in the foregut and differentiate into enteric neurons, but subsequently undergo apoptosis resulting in a striking neuronal deficit. Moreover, ectopic expression of the transcription factor meis3 and/or the receptor ret, partially rescues enteric neuron colonization after RA attenuation. Collectively, our findings suggest that retinoic acid plays a critical temporal role in promoting enteric neural crest chain migration and neuronal survival upstream of Meis3 and RET in vivo.", "date": "2018-01-01", "date_type": "published", "publication": "Developmental Biology", "volume": "433", "number": "1", "publisher": "Elsevier", "pagerange": "17-32", "id_number": "CaltechAUTHORS:20171106-091459396", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171106-091459396", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Burroughs Wellcome Fund" }, { "agency": "NIH Predoctoral Fellowship", "grant_number": "HD080343" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "Caltech Beckman Institute" } ] }, "doi": "10.1016/j.ydbio.2017.10.021", "pmcid": "PMC5722660", "primary_object": { "basename": "FigS1.jpg", "url": "https://authors.library.caltech.edu/records/cd2pp-drm03/files/FigS1.jpg" }, "related_objects": [ { "basename": "FigS2.jpg", "url": "https://authors.library.caltech.edu/records/cd2pp-drm03/files/FigS2.jpg" }, { "basename": "nihms919756.pdf", "url": "https://authors.library.caltech.edu/records/cd2pp-drm03/files/nihms919756.pdf" } ], "resource_type": "article", "pub_year": "2018", "author_list": "Uribe, Rosa A.; Hong, Stephanie S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mttdh-m5x67", "eprint_id": 85693, "eprint_status": "archive", "datestamp": "2023-08-19 07:02:30", "lastmod": "2023-10-18 18:40:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Insights into neural crest development from studies of avian embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, migration, genetic toolbox, multipotent, neural plate border", "note": "\u00a9 2018 UPV/EHU Press. \n\nSubmitted: 24 January, 2018; Accepted: 24 January, 2018.\n\nPublished - ft183.pdf
", "abstract": "The neural crest is a multipotent and highly migratory cell type that contributes to many of the defining features of vertebrates, including the skeleton of the head and most of the peripheral nervous system. 150 years after the discovery of the neural crest, avian embryos remain one of the most important model organisms for studying neural crest development. In this review, we describe aspects of neural crest induction, migration and axial level differences, highlighting what is known about the underlying gene regulatory mechanisms. Past and emerging technologies continue to improve the resolution with which we can examine important questions of neural crest development, with modern avian molecular embryology continuing to make important contributions.", "date": "2018", "date_type": "published", "publication": "International Journal of Developmental Biology", "volume": "62", "number": "1-3", "publisher": "University of the Basque Country Press", "pagerange": "183-194", "id_number": "CaltechAUTHORS:20180409-140111565", "issn": "0214-6282", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20180409-140111565", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1387/ijdb.180038sg", "primary_object": { "basename": "ft183.pdf", "url": "https://authors.library.caltech.edu/records/mttdh-m5x67/files/ft183.pdf" }, "resource_type": "article", "pub_year": "2018", "author_list": "Gandhi, Shashank and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zqd1x-gme87", "eprint_id": 83219, "eprint_status": "archive", "datestamp": "2023-08-21 22:20:51", "lastmod": "2023-10-17 22:59:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gandhi-Shashank", "name": { "family": "Gandhi", "given": "Shashank" }, "orcid": "0000-0002-4081-4338" }, { "id": "Piacentino-Michael-L", "name": { "family": "Piacentino", "given": "Michael L." }, "orcid": "0000-0003-1773-031X" }, { "id": "Vieceli-Felipe-M", "name": { "family": "Vieceli", "given": "Felipe M." }, "orcid": "0000-0001-5142-8224" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "CRISPR/Cas9; Chick embryos; gRNA; Neural crest; Knockout", "note": "\u00a9 2017 Elsevier Inc. \n\nReceived 23 May 2017, Revised 26 August 2017, Accepted 29 August 2017, Available online 14 November 2017. \n\nWe would like to thank Alberto Stolfi from Lionel Christiaen's lab (New York University) and Chao-Yuan Yeh from Cheng-Ming Chuong's lab (University of Southern California) for reagents, Erica Hutchins for technical assistance, Yuwei Li, Meyer Barembaum and the rest of the Bronner lab for valuable discussions on the project, Elena K. Perry for helpful comments on the manuscript, the Caltech Biological Imaging Facility for technical assistance on imaging, and Jamie Tijerina with the Caltech Flow Cytometry Facility for assistance with flow cytometry. This work was partially supported by the National Institutes of Health (NIH) [R01 DE024157 to MEB and F32 HD088022 to MLP] and the Brazilian National Council for Scientific and Technological Development [PDE 207656/2014-2 to FMV]. \n\nAuthor contributions: S.G. and M.E.B. conceived this study. S.G., M.L.P., F.M.V., and M.E.B. designed the experiments. S.G., M.L.P. and F.M.V performed the experiments and analyzed the results. S.G., M.L.P. and M.E.B. wrote the manuscript. \n\nThe authors declare no competing or financial interests.\n\nAccepted Version - nihms911633.pdf
Supplemental Material - mmc1.docx
", "abstract": "The advent of CRISPR/Cas9 has made genome editing possible in virtually any organism, including those not previously amenable to genetic manipulations. Here, we present an optimization of CRISPR/Cas9 for application to early avian embryos with improved efficiency via a three-fold strategy. First, we employed Cas9 protein flanked with two nuclear localization signal sequences for improved nuclear localization. Second, we used a modified guide RNA (gRNA) scaffold that obviates premature termination of transcription and unstable Cas9-gRNA interactions. Third, we used a chick-specific U6 promoter that yields 4-fold higher gRNA expression than the previously utilized human U6. For rapid screening of gRNAs for in vivo applications, we also generated a chicken fibroblast cell line that constitutively expresses Cas9. As proof of principle, we performed electroporation-based loss-of-function studies in the early chick embryo to knock out Pax7 and Sox10, key transcription factors with known functions in neural crest development. The results show that CRISPR/Cas9-mediated deletion causes loss of their respective proteins and transcripts, as well as predicted downstream targets. Taken together, the results reveal the utility of this optimized CRISPR/Cas9 method for targeted gene knockout in chicken embryos in a manner that is reproducible, robust and specific.", "date": "2017-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "432", "number": "1", "publisher": "Elsevier", "pagerange": "86-97", "id_number": "CaltechAUTHORS:20171115-100516790", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171115-100516790", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE024157" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32 HD088022" }, { "agency": "Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)", "grant_number": "PDE 207656/2014-2" } ] }, "doi": "10.1016/j.ydbio.2017.08.036", "pmcid": "PMC5728388", "primary_object": { "basename": "mmc1.docx", "url": "https://authors.library.caltech.edu/records/zqd1x-gme87/files/mmc1.docx" }, "related_objects": [ { "basename": "nihms911633.pdf", "url": "https://authors.library.caltech.edu/records/zqd1x-gme87/files/nihms911633.pdf" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Gandhi, Shashank; Piacentino, Michael L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/030t5-ngz04", "eprint_id": 83495, "eprint_status": "archive", "datestamp": "2023-08-19 06:10:45", "lastmod": "2023-10-17 23:10:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lignell-Antti", "name": { "family": "Lignell", "given": "Antti" }, "orcid": "0000-0001-7664-5583" }, { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Streichan-Sebastian-J", "name": { "family": "Streichan", "given": "Sebastian J." }, "orcid": "0000-0002-6105-9087" }, { "id": "Cai-Long", "name": { "family": "Cai", "given": "Long" }, "orcid": "0000-0002-7154-5361" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Identification of a neural crest stem cell niche by Spatial Genomic Analysis", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nReceived: 11 July 2017; Accepted: 27 September 2017; Published online: 28 November 2017. \n\nThis work was supported by Grants HD037105 to M.E.B. and HD075605, 1DP2OD008530, and 5R21DA038468 to L.C., as well as fellowships from Jane and Aatos Erkko Foundation, Ella and Georg Ehrnrooth Foundation, and V\u00e4re Foundation to L.K. \n\nAuthor Contributions: L.K. and A.L. conceived the project. A.L. and L.K. designed the experiments, and L.C. and M.E.B. supervised the research. L.K. and A.L. analyzed the data. SGA was developed by A.L. and L.C. together with L.K. Sample preparation was performed by L.K., single molecule fluorescent in situ hybridization protocol and imaging was performed by A.L., and the data analysis pipeline was developed by A.L. and S.J.S. The paper was written by L.K., A.L., M.E.B. and L.C. \n\nThe authors declare no competing financial interests.\n\nPublished - s41467-017-01561-w.pdf
Supplemental Material - 41467_2017_1561_MOESM1_ESM.pdf
Supplemental Material - 41467_2017_1561_MOESM2_ESM.docx
Supplemental Material - 41467_2017_1561_MOESM3_ESM.mp4
", "abstract": "The neural crest is an embryonic population of multipotent stem cells that form numerous defining features of vertebrates. Due to lack of reliable techniques to perform transcriptional profiling in intact tissues, it remains controversial whether the neural crest is a heterogeneous or homogeneous population. By coupling multiplex single molecule fluorescence in situ hybridization with machine learning algorithm based cell segmentation, we examine expression of 35 genes at single cell resolution in vivo. Unbiased hierarchical clustering reveals five spatially distinct subpopulations within the chick dorsal neural tube. Here we identify a neural crest stem cell niche that centers around the dorsal midline with high expression of neural crest genes, pluripotency factors, and lineage markers. Interestingly, neural and neural crest stem cells express distinct pluripotency signatures. This Spatial Genomic Analysis toolkit provides a straightforward approach to study quantitative multiplex gene expression in numerous biological systems, while offering insights into gene regulatory networks via synexpression analysis.", "date": "2017-11-28", "date_type": "published", "publication": "Nature Communications", "volume": "8", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 1830", "id_number": "CaltechAUTHORS:20171128-101613048", "issn": "2041-1723", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171128-101613048", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "HD075605" }, { "agency": "NIH", "grant_number": "1DP2OD008530" }, { "agency": "NIH", "grant_number": "5R21DA038468" }, { "agency": "Jane and Aatos Erkko Foundation" }, { "agency": "Ella and Georg Ehrnrooth Foundation" }, { "agency": "V\u00e4re Foundation" } ] }, "doi": "10.1038/s41467-017-01561-w", "pmcid": "PMC5705662", "primary_object": { "basename": "41467_2017_1561_MOESM3_ESM.mp4", "url": "https://authors.library.caltech.edu/records/030t5-ngz04/files/41467_2017_1561_MOESM3_ESM.mp4" }, "related_objects": [ { "basename": "s41467-017-01561-w.pdf", "url": "https://authors.library.caltech.edu/records/030t5-ngz04/files/s41467-017-01561-w.pdf" }, { "basename": "41467_2017_1561_MOESM1_ESM.pdf", "url": "https://authors.library.caltech.edu/records/030t5-ngz04/files/41467_2017_1561_MOESM1_ESM.pdf" }, { "basename": "41467_2017_1561_MOESM2_ESM.docx", "url": "https://authors.library.caltech.edu/records/030t5-ngz04/files/41467_2017_1561_MOESM2_ESM.docx" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Lignell, Antti; Kerosuo, Laura; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/c3t2g-qdw03", "eprint_id": 83887, "eprint_status": "archive", "datestamp": "2023-08-19 05:58:06", "lastmod": "2023-10-18 14:28:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Huang-Miller", "name": { "family": "Huang", "given": "Miller" } }, { "id": "Zheng-Tina", "name": { "family": "Zheng", "given": "Tina" } }, { "id": "Guo-Jeffrey", "name": { "family": "Guo", "given": "Jeffrey" } }, { "id": "Sperring-C", "name": { "family": "Sperring", "given": "Colin" } }, { "id": "Miller-M", "name": { "family": "Miller", "given": "Matthew" } }, { "id": "McHenry-L-K", "name": { "family": "McHenry", "given": "Lauren" } }, { "id": "Zhen-Qiqi", "name": { "family": "Zhen", "given": "Qiqi" } }, { "id": "Moriarity-B-S", "name": { "family": "Moriarity", "given": "Branden" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Conklin-B-R", "name": { "family": "Conklin", "given": "Bruce" } }, { "id": "Largaespada-D-A", "name": { "family": "Largaespada", "given": "David" } }, { "id": "Maris-J-M", "name": { "family": "Maris", "given": "John" } }, { "id": "Matthay-K-K", "name": { "family": "Matthay", "given": "Katherine" } }, { "id": "Weiss-W-A", "name": { "family": "Weiss", "given": "William" } } ] }, "title": "Human pluripotent stem cell-based models of neuroblastoma", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 The Author(s). Published by Oxford University Press on behalf of the Society for Neuro-Oncology. \n\nPublished: 06 November 2017.", "abstract": "Neuroblastoma (NB) is a malignant tumor that accounts for ~15% of all pediatric cancer mortality. Nearly half of patients present with high-risk disease that has poor outcome, and the proto-oncogene MYCN is amplified in 45% of high-risk cases. Further, relapsed neuroblastoma demonstrates frequent activation of mitogen activated protein kinase (MAPK) signaling, including mutations in tumor suppressor NF1 and epigenetic silencing of the gene. Human induced pluripotent stem cells (iPSCs) represent a valuable tool by which to further dissect the genetic requirements of high-risk NB. Human iPSCs are superior to genetically engineered mouse models (GEMMs) at capturing the telomere biology and chromosomal landscape of primary human neuroblastoma. Further, they can be genetically manipulated to determine the genetic drivers of the disease. As proof of principle, we first differentiated normal human iPSCs toward trunk neural crest cells (NCC), the putative cells of origin for NB. We subsequently introduced established genetic drivers of NB (MYCN, ALK) and orthotopically implanted the resulting trunk NCCs into the renal capsules of immunocompromised mice. Three months post injection, tumors developed in 60% of MYCN/ALK mice, 10% in MYCN alone, and no tumors developed in ALK alone or empty vector. Tumors were transplantable and expressed markers typically found in NB, while lacking markers of other tumors such as rhabdomyosarcoma, Ewing sarcoma, and lymphoma. To show we can use our model to evaluate candidate drivers of NB, we knocked out NF1 in MYCN trunk NCCs using CRISPR/Cas9 and implanted these cells orthotopically in mice. 80% of mice with MYCN/NF1 modified cells developed tumors while NF1 knockout alone did not. Thus, we have generated the first human iPSC model of NB driven by MYCN and have shown the utility of our model in validating cooperating mutations. MH and TZ contributed equally to this work.", "date": "2017-11-06", "date_type": "published", "publication": "Neuro Oncology", "volume": "19", "number": "S6", "publisher": "Oxford University Press", "pagerange": "256", "id_number": "CaltechAUTHORS:20171213-141234017", "issn": "1522-8517", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171213-141234017", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1093/neuonc/nox168.1048", "resource_type": "article", "pub_year": "2017", "author_list": "Huang, Miller; Zheng, Tina; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/21w29-40423", "eprint_id": 82583, "eprint_status": "archive", "datestamp": "2023-08-19 05:40:08", "lastmod": "2023-10-17 22:32:19", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Suarez-Bregua-P", "name": { "family": "Suarez-Bregua", "given": "Paula" } }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Rotllant-J", "name": { "family": "Rotllant", "given": "Josep" } } ] }, "title": "Targeted Pth4-expressing cell ablation impairs skeletal mineralization in zebrafish", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 Suarez-Bregua et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived: June 1, 2017; Accepted: October 2, 2017; Published: October 17, 2017. \n\nData Availability Statement: All relevant data are within the paper. \n\nThis work was funded by the Spanish Economy and Competitiveness Ministry project ALG2011-23581 and AGL2014-52473R to JR. AS was supported by a National Institutes of Health (NIH) grant R01DE024157 and PSB was supported by a Campus do Mar PhD grant, Xunta de Galicia (PRE/2012/532) and AGL2014-52473R project contract. \n\nThe authors have declared that no competing interests exist.\n\nAuthor Contributions: \nFormal analysis: Paula Suarez-Bregua.\nFunding acquisition: Josep Rotllant.\nInvestigation: Paula Suarez-Bregua, Ankur Saxena, Marianne E. Bronner, Josep Rotllant.\nMethodology: Paula Suarez-Bregua, Ankur Saxena.\nProject administration: Marianne E. Bronner, Josep Rotllant.\nSupervision: Ankur Saxena, Marianne E. Bronner, Josep Rotllant.\nWriting \u00b1 original draft: Paula Suarez-Bregua.\nWriting \u00b1 review & editing: Ankur Saxena, Marianne E. Bronner, Josep Rotllant.\n\nPublished - journal.pone.0186444.pdf
", "abstract": "Skeletal development and mineralization are essential processes driven by the coordinated action of neural signals, circulating molecules and local factors. Our previous studies revealed that the novel neuropeptide Pth4, synthesized by hypothalamic cells, was involved in bone metabolism via phosphate regulation in adult zebrafish. Here, we investigate the role of pth4 during skeletal development using single-cell resolution, two-photon laser ablation of Pth4:eGFP-expressing cells and confocal imaging in vivo. Using a stable transgenic Pth4:eGFP zebrafish line, we identify Pth4:eGFP-expressing cells as post-mitotic neurons. After targeted ablation of eGFP-expressing cells in the hypothalamus, the experimental larvae exhibited impaired mineralization of the craniofacial bones whereas cartilage development was normal. In addition to a decrease in pth4 transcript levels, we noted altered expression of phex and entpd5, genes associated with phosphate homeostasis and mineralization, as well as a delay in the expression of osteoblast differentiation markers such as sp7 and sparc. Taken together, these results suggest that Pth4-expressing hypothalamic neurons participate in the regulation of bone metabolism, possibly through regulating phosphate balance during zebrafish development.", "date": "2017-10-17", "date_type": "published", "publication": "PLoS ONE", "volume": "12", "number": "10", "publisher": "Public Library of Science", "pagerange": "Art. No. e0186444", "id_number": "CaltechAUTHORS:20171023-111406236", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171023-111406236", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Ministerio de Econom\u00eda, Industria y Competitividad (MINECO)", "grant_number": "ALG2011-23581" }, { "agency": "Ministerio de Econom\u00eda, Industria y Competitividad (MINECO)", "grant_number": "AGL2014-52473R" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "Xunta de Galicia", "grant_number": "PRE/2012/532" } ] }, "doi": "10.1371/journal.pone.0186444", "pmcid": "PMC5645135", "primary_object": { "basename": "journal.pone.0186444.pdf", "url": "https://authors.library.caltech.edu/records/21w29-40423/files/journal.pone.0186444.pdf" }, "resource_type": "article", "pub_year": "2017", "author_list": "Suarez-Bregua, Paula; Saxena, Ankur; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7hx2y-9qs58", "eprint_id": 82402, "eprint_status": "archive", "datestamp": "2023-08-19 05:35:41", "lastmod": "2023-10-17 22:15:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Li-Ang", "name": { "family": "Li", "given": "Ang" } }, { "id": "Junge-J", "name": { "family": "Junge", "given": "Jason" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Planar cell polarity signaling coordinates oriented cell division and cell rearrangement in clonally expanding growth plate cartilage", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 Copyright Li et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. \n\nReceived: 14 November 2016; Accepted: 15 September 2017; Published: 10 October 2017. \n\nThis project is supported by DE024157 to MEB We thank Professor Carlos Lois, Professor Cheng-Ming Chuong and Caltech Biological Imaging Facility for sharing equipment. \n\nThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. \n\nCompeting interests: Marianne Bronner: Senior Editor, eLife. The other authors declare that no competing interests exist. \n\nAuthor contributions: Yuwei Li, Conceived and designed the project, Constructed reagents and performed experiments, Performed quantitative analyses, Wrote the manuscript; Ang Li, Performed quantitative and statistical analysis, Helpful discussion; Jason Junge, Performed bioinformatic analyses of Frizzled-7 and Dishevelled-2 proteins, Helpful discussion; Marianne Bronner, Funding acquisition, Conceived and designed the project, Wrote the manuscript.\n\nPublished - elife-23279-v1.pdf
", "abstract": "Both oriented cell divisions and cell rearrangements are critical for proper embryogenesis and organogenesis. However, little is known about how these two cellular events are integrated. Here we examine the linkage between these processes in chick limb cartilage. By combining retroviral-based multicolor clonal analysis with live imaging, the results show that single chondrocyte precursors can generate both single-column and multi-column clones through oriented division followed by cell rearrangements. Focusing on single column formation, we show that this stereotypical tissue architecture is established by a pivot-like process between sister cells. After mediolateral cell division, N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other, resulting in stacking into a column. Perturbation analyses demonstrate that planar cell polarity signaling enables cells to pivot in the direction of limb elongation via this N-cadherin-mediated coupling. Our work provides new insights into the mechanisms generating appropriate tissue architecture of limb skeleton.", "date": "2017-10-10", "date_type": "published", "publication": "eLife", "volume": "6", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e23279", "id_number": "CaltechAUTHORS:20171017-080224707", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20171017-080224707", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" } ] }, "doi": "10.7554/eLife.23279", "pmcid": "PMC5634781", "primary_object": { "basename": "elife-23279-v1.pdf", "url": "https://authors.library.caltech.edu/records/7hx2y-9qs58/files/elife-23279-v1.pdf" }, "resource_type": "article", "pub_year": "2017", "author_list": "Li, Yuwei; Li, Ang; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/e8xxw-enp74", "eprint_id": 80833, "eprint_status": "archive", "datestamp": "2023-08-19 05:17:55", "lastmod": "2023-10-17 18:31:26", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Martik-Megan-L", "name": { "family": "Martik", "given": "Megan L." }, "orcid": "0000-0003-1186-4085" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Regulatory Logic Underlying Diversification of the Neural Crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 Elsevier Ltd. \n\nAvailable online 26 August 2017. \n\nWork in the laboratory of M.E.B. is supported by NIHR01DE02415, R01NS08690, and HD037105. M.L.M. is funded by the Helen Hay Whitney Foundation.\n\nAccepted Version - nihms902379.pdf
", "abstract": "The neural crest is a transient, multipotent population of cells that arises at the border of the developing nervous system. After closure of the neural tube, these cells undergo an epithelial-to-mesenchymal transition (EMT) to delaminate and migrate, often to distant locations in the embryo. Neural crest cells give rise to a diverse array of derivatives including neurons and glia of the peripheral nervous system, melanocytes, and bone and cartilage of the face. A gene regulatory network (GRN) controls the specification, delamination, migration, and differentiation of this fascinating cell type. With increasing technological advances, direct linkages within the neural crest GRN are being uncovered. The underlying circuitry is useful for understanding important topics such as reprogramming, evolution, and disease.", "date": "2017-10", "date_type": "published", "publication": "Trends in Genetics", "volume": "33", "number": "10", "publisher": "Cell Press", "pagerange": "715-727", "id_number": "CaltechAUTHORS:20170828-082452759", "issn": "0168-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170828-082452759", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE02415" }, { "agency": "NIH", "grant_number": "R01NS08690" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Helen Hay Whitney Foundation" } ] }, "doi": "10.1016/j.tig.2017.07.015", "pmcid": "PMC5610108", "primary_object": { "basename": "nihms902379.pdf", "url": "https://authors.library.caltech.edu/records/e8xxw-enp74/files/nihms902379.pdf" }, "resource_type": "article", "pub_year": "2017", "author_list": "Martik, Megan L. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0qv4z-cx968", "eprint_id": 74061, "eprint_status": "archive", "datestamp": "2023-08-21 20:55:13", "lastmod": "2023-10-24 22:03:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bajpai-Vivek-K", "name": { "family": "Bajpai", "given": "Vivek K." } }, { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Tseropoulos-Georgios", "name": { "family": "Tseropoulos", "given": "Georgios" } }, { "id": "Cummings-Kirstie-A", "name": { "family": "Cummings", "given": "Kirstie A." } }, { "id": "Wang-Xiaoyan", "name": { "family": "Wang", "given": "Xiaoyan" } }, { "id": "Lei-Pedro", "name": { "family": "Lei", "given": "Pedro" } }, { "id": "Liu-Biao", "name": { "family": "Liu", "given": "Biao" } }, { "id": "Liu-Song", "name": { "family": "Liu", "given": "Song" } }, { "id": "Popescu-Gabriela", "name": { "family": "Popescu", "given": "Gabriela" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Andreadis-Stelios-T", "name": { "family": "Andreadis", "given": "Stelios T." } } ] }, "title": "Reprogramming Postnatal Human Epidermal Keratinocytes toward Functional Neural Crest Fates", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Epidermal keratinocytes; Neural plate border; Neural crest induction; Reprogramming", "note": "\u00a9 2017 AlphaMed Press. \n\nAccepted manuscript online: 31 January 2017; Manuscript Accepted: 7 January 2017; Manuscript Revised: 5 December 2016; Manuscript Received: 31 May 2016. \n\nThis work was supported by grants from the University at Buffalo (IMPACT Award) and National Institutes of Health (R01EB023114) to S.T.A. K.A.C. was supported by Grant F31 NS 084668. G.P. was supported by Grants F31 NS 084668 and AHA 12EIA9100012. M.E.B. was supported by R01DE024157. We thank Deepika Verma for her help in creating schematic diagram. \n\nAuthor Contributions: V.K.B.: conceptualized and designed the study and performed the experiments; V.K.B. and S.T.A.: designed experiments and performed data analysis and interpretation; L.K. and M.E.B.: performed chicken embryo experiments; K.A.C. and G.P.: electrophysiological analysis; G.T., X.W., and P.L.: performed experiments; B.L., S.L., and V.K.B.: RNA sequencing analysis. V.K.B., S.T.A., and M.E.B.: wrote the manuscript; All authors read the manuscript and provided their inputs. \n\nAccession Numbers: GEO: GSE72268 \n\nThe authors indicate no potential conflicts of interest.\n\nAccepted Version - nihms882815.pdf
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Supplemental Material - stem2583-sup-0007-suppinfoFig5.tiff
", "abstract": "During development, neural crest cells are induced by signaling events at the neural plate border of all vertebrate embryos. Initially arising within the central nervous system, neural crest cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. Here we provide evidence that postnatal human epidermal keratinocytes, in response to FGF2 and IGF1 signals, can be reprogrammed toward a neural crest fate. Genome-wide transcriptome analyses show that keratinocyte-derived neural crest cells are similar to those derived from human embryonic stem cells. Moreover, they give rise in vitro and in vivo to neural crest derivatives such as peripheral neurons, melanocytes, Schwann cells and mesenchymal cells (osteocytes, chondrocytes, adipocytes and smooth muscle). By demonstrating that human KRT14+ keratinocytes can form neural crest cells, even from clones of single cells, our results have important implications in stem cell biology and regenerative medicine.", "date": "2017-05", "date_type": "published", "publication": "Stem Cells", "volume": "35", "number": "5", "publisher": "Wiley", "pagerange": "1402-1415", "id_number": "CaltechAUTHORS:20170206-083642837", "issn": "1066-5099", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170206-083642837", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "University at Buffalo" }, { "agency": "NIH", "grant_number": "R01EB023114" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH", "grant_number": "AHA 12EIA9100012" } ] }, "doi": "10.1002/stem.2583", "pmcid": "PMC5543412", "primary_object": { "basename": "stem2583-sup-0004-suppinfoFig2.tiff", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0004-suppinfoFig2.tiff" }, "related_objects": [ { "basename": "stem2583-sup-0005-suppinfoFig3.tiff", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0005-suppinfoFig3.tiff" }, { "basename": "stem2583-sup-0006-suppinfoFig4.tiff", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0006-suppinfoFig4.tiff" }, { "basename": "stem2583-sup-0007-suppinfoFig5.tiff", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0007-suppinfoFig5.tiff" }, { "basename": "nihms882815.pdf", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/nihms882815.pdf" }, { "basename": "stem2583-sup-0001-suppinfo1.pdf", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0001-suppinfo1.pdf" }, { "basename": "stem2583-sup-0002-suppinfo2.docx", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0002-suppinfo2.docx" }, { "basename": "stem2583-sup-0003-suppinfoFig1.tiff", "url": "https://authors.library.caltech.edu/records/0qv4z-cx968/files/stem2583-sup-0003-suppinfoFig1.tiff" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Bajpai, Vivek K.; Kerosuo, Laura; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/m0yn9-zdm51", "eprint_id": 73681, "eprint_status": "archive", "datestamp": "2023-08-19 02:33:20", "lastmod": "2023-10-24 15:36:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Uy-Benjamin-R", "name": { "family": "Uy", "given": "Benjamin R." }, "orcid": "0000-0003-0438-880X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ancient evolutionary origin of vertebrate enteric neurons from trunk-derived neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 Macmillan Publishers Limited. \n\nreceived 23 February 2016; accepted 31 January 2017. Published online 20 March 2017. \n\nWe thank C. Baker, M. Piacentino, and L. Kerosuo for discussion, and M. Martik, M. Simoes-Costa, and R. Uribe for their comments on this manuscript. \n\nThese authors contributed equally to this work: Stephen A. Green & Benjamin R. Uy. \n\nAuthor Contributions: The project was conceived by M.E.B., and analyses were designed by S.A.G. and M.E.B. Descriptive analyses of enteric neurons were performed by S.A.G. Cranial DiI labelling was performed by B.R.U., M.E.B., and S.A.G. Trunk DiI labelling was performed by B.R.U., S.A.G. and M.E.B. Surgeries were performed, imaged and analysed by S.A.G. and B.R.U. Schematics were drawn by S.A.G. The manuscript was written by M.E.B., S.A.G. and B.R.U. \n\nData availability: The datasets generated during and/or analysed during the current study are available from the corresponding author upon reasonable request. \n\nThe authors declare no competing financial interests.\n\nAccepted Version - nihms848562.pdf
Supplemental Material - nature21679-sf1.jpg
Supplemental Material - nature21679-sf2.jpg
", "abstract": "The enteric nervous system of jawed vertebrates arises primarily from vagal neural crest cells that migrate to the foregut and subsequently colonize and innervate the entire gastrointestinal tract. Here we examine development of the enteric nervous system in the basal jawless vertebrate the sea lamprey (Petromyzon marinus) to gain insight into its evolutionary origin. Surprisingly, we find no evidence for the existence of a vagally derived enteric neural crest population in the lamprey. Rather, labelling with the lipophilic dye DiI shows that late-migrating cells, originating from the trunk neural tube and associated with nerve fibres, differentiate into neurons within the gut wall and typhlosole. We propose that these trunk-derived neural crest cells may be homologous to Schwann cell precursors, recently shown in mammalian embryos to populate post-embryonic parasympathetic ganglia, including enteric ganglia. Our results suggest that neural-crest-derived Schwann cell precursors made an important contribution to the ancient enteric nervous system of early jawless vertebrates, a role that was largely subsumed by vagal neural crest cells in early gnathostomes.", "date": "2017-04-06", "date_type": "published", "publication": "Nature", "volume": "544", "number": "7648", "publisher": "Nature Publishing Group", "pagerange": "88-91", "id_number": "CaltechAUTHORS:20170124-161810111", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170124-161810111", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechAUTHORS", "doi": "10.1038/nature21679", "pmcid": "PMC5383518", "primary_object": { "basename": "nature21679-sf1.jpg", "url": "https://authors.library.caltech.edu/records/m0yn9-zdm51/files/nature21679-sf1.jpg" }, "related_objects": [ { "basename": "nature21679-sf2.jpg", "url": "https://authors.library.caltech.edu/records/m0yn9-zdm51/files/nature21679-sf2.jpg" }, { "basename": "nihms848562.pdf", "url": "https://authors.library.caltech.edu/records/m0yn9-zdm51/files/nihms848562.pdf" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Green, Stephen A.; Uy, Benjamin R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cyf37-yqw98", "eprint_id": 75646, "eprint_status": "archive", "datestamp": "2023-08-19 02:12:11", "lastmod": "2023-10-25 15:11:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Roellig-D", "name": { "family": "Roellig", "given": "Daniela" }, "orcid": "0000-0002-7558-3592" }, { "id": "Tan-Cabugao-J", "name": { "family": "Tan-Cabugao", "given": "Johanna" } }, { "id": "Esaian-S", "name": { "family": "Esaian", "given": "Sevan" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2017 Roellig et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. \n\nReceived: 17 September 2016; Accepted: 14 March 2017; Published: 29 March 2017. \n\nWe thank Bertrand B\u00e9naz\u00e9raf for helpful discussions and critical reading of the manuscript. We thank Laura Kerosuo and Erica J Hutchins for advice on cell culture experiments and comments on the manuscript. We are grateful to Fabienne Pituello for providing work space in her lab for revisions. We thank Hisato Kondoh and Fernando Giraldez for the generous gift of the pCaggs-cSox2 construct, Marcos Simoes-Costa for the gift of pCI-Pax7-IRES-H2B-RFP, Martin Cheung for pCaggs-Sox1-GFP and Michael R Stark for the gift of the pCI-Pax3-H2B-GFP plasmid. SE was funded by the CIRM Bridges Training Grant #TB1-01176. \n\nAuthor contributions: DR, Conceptualization, Data curation, Formal analysis, Supervision, Validation, Visualization, Methodology, Writing\u2014original draft, Project administration, Writing\u2014review and editing; JT-C, SE, Investigation, Visualization; MEB, Conceptualization, Resources, Supervision, Funding acquisition, Writing\u2014original draft, Project administration, Writing\u2014review and editing. \n\nCompeting interests: MEB: Senior editor, eLife. The other authors declare that no competing interests exist.\n\nPublished - e21620-download.pdf
", "abstract": "The 'neural plate border' of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure.", "date": "2017-03-29", "date_type": "published", "publication": "eLife", "volume": "6", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e21620", "id_number": "CaltechAUTHORS:20170403-134019209", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170403-134019209", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "TB1-01176" } ] }, "collection": "CaltechAUTHORS", "doi": "10.7554/eLife.21620", "pmcid": "PMC5371430", "primary_object": { "basename": "e21620-download.pdf", "url": "https://authors.library.caltech.edu/records/cyf37-yqw98/files/e21620-download.pdf" }, "resource_type": "article", "pub_year": "2017", "author_list": "Roellig, Daniela; Tan-Cabugao, Johanna; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0desp-edx07", "eprint_id": 75070, "eprint_status": "archive", "datestamp": "2023-08-19 01:58:13", "lastmod": "2023-10-25 14:43:08", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Chen-Jingchen", "name": { "family": "Chen", "given": "Jingchen" } }, { "id": "Tambalo-Monica", "name": { "family": "Tambalo", "given": "Monica" } }, { "id": "Barembaum-Meyer", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Ranganathan-Ramya", "name": { "family": "Ranganathan", "given": "Ramya" } }, { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos S." }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Streit-Andrea", "name": { "family": "Streit", "given": "Andrea" }, "orcid": "0000-0001-7664-7917" } ] }, "title": "A systems level approach reveals new gene regulatory modules in the developing ear", "ispublished": "pub", "full_text_status": "public", "keywords": "auditory system, cell fate, chick, embryo, hearing, placode, transcription factor", "note": "\u00a9 2017 Published by The Company of Biologists Ltd.\nThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.\n\nReceived December 20, 2016; Accepted February 24, 2017.\n\nFunding:\nThis study was funded by grants from the Biotechnology and Biological Sciences Research Council (BB/I021647/1), Deafness Research UK (513:KCL:AS) and the National Institute on Deafness and Other Communication Disorders (DC011577). Deposited in PMC for immediate release.\n\nAuthor contributions:\nA.S. designed the experiments; J.C. and M.T. collected otic tissues; R.R. performed RNAseq for PPR tissue; J.C. analysed RNAseq data; J.C. and M.T. performed most functional experiments and analysed all data together with A.S.; M.B. contributed to the knockdown experiments; M.S.-C. contributed to RNAseq experiments; J.C., A.S. and M.E.B. wrote the manuscript.\n\nCompeting interests:\nThe authors declare no competing or financial interests.\n\nData availability:\nAll sequencing data have been deposited in Gene Expression Omnibus under accession number GSE69185 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE69185).\n\nPublished - 1531.full.pdf
Supplemental Material - DEV148494supp.pdf
", "abstract": "The inner ear is a complex vertebrate sense organ, yet it arises from a simple epithelium, the otic placode. Specification towards otic fate requires diverse signals and transcriptional inputs that act sequentially and/or in parallel. Using the chick embryo, we uncover novel genes in the gene regulatory network underlying otic commitment and reveal dynamic changes in gene expression. Functional analysis of selected transcription factors reveals the genetic hierarchy underlying the transition from progenitor to committed precursor, integrating known and novel molecular players. Our results not only characterize the otic transcriptome in unprecedented detail, but also identify new gene interactions responsible for inner ear development and for the segregation of the otic lineage from epibranchial progenitors. By recapitulating the embryonic programme, the genes and genetic sub-circuits discovered here might be useful for reprogramming na\u00efve cells towards otic identity to restore hearing loss.", "date": "2017-03-06", "date_type": "published", "publication": "Development", "volume": "144", "number": "8", "publisher": "Company of Biologists", "pagerange": "1531-1543", "id_number": "CaltechAUTHORS:20170313-142540623", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170313-142540623", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "BB/I021647/1" }, { "agency": "Deafness Research (UK)", "grant_number": "513:KCL:AS" }, { "agency": "NIH", "grant_number": "DC011577" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1242/dev.148494", "pmcid": "PMC5399671", "primary_object": { "basename": "DEV148494supp.pdf", "url": "https://authors.library.caltech.edu/records/0desp-edx07/files/DEV148494supp.pdf" }, "related_objects": [ { "basename": "1531.full.pdf", "url": "https://authors.library.caltech.edu/records/0desp-edx07/files/1531.full.pdf" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Chen, Jingchen; Tambalo, Monica; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/p20yt-8zt50", "eprint_id": 73174, "eprint_status": "archive", "datestamp": "2023-08-22 19:39:31", "lastmod": "2023-10-24 15:05:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Murko-Christina", "name": { "family": "Murko", "given": "Christina" }, "orcid": "0000-0003-2281-0031" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Tissue specific regulation of the chick Sox10E1 enhancer by different Sox family members", "ispublished": "pub", "full_text_status": "public", "keywords": "Chick: Sox10; Neural crest; Otic", "note": "\u00a9 2016 Elsevier Inc. \n\nReceived 16 June 2016; received in revised form 2 December 2016; accepted 3 December 2016; available online 22 December 2016. \n\nWe thank M. Barembaum and M. Sim\u00f5es-Costa for plasmids and morpholinos. This work was supported by grants DE024157 and HD037105 from NIH to MEB and a postdoctoral fellowship from the Curci foundation and an Erwin Schr\u00f6dinger fellowship from the Austrian Science Fund FWF (J3538-B19) to CM.\n\nAccepted Version - nihms939439.pdf
Supplemental Material - mmc1.docx
", "abstract": "The transcription factor Sox10 is a key regulator of vertebrate neural crest development and serves crucial functions in the differentiation of multiple neural crest lineages. In the chick neural crest, two cis-regulatory elements have been identified that mediate Sox10 expression: Sox10E2, which initiates expression in cranial neural crest; Sox10E1 driving expression in vagal and trunk neural crest. Both also mediate Sox10 expression in the otic placode. Here, we have dissected and analyzed the Sox10E1 enhancer element to identify upstream regulatory inputs. Via mutational analysis, we found two critical Sox sites with differential impact on trunk versus otic Sox10E1 mediated reporter expression. Mutation of a combined SoxD/E motif was sufficient to completely abolish neural crest but not ear enhancer activity. However, mutation of both the SoxD/E and another SoxE site eliminated otic Sox10E1 expression. Loss-of-function experiments reveal Sox5 and Sox8 as critical inputs for trunk neural crest enhancer activity, but only Sox8 for its activity in the ear. Finally, we show by ChIP and co-immunoprecipitation that Sox5 directly binds to the SoxD/E site, and that it can interact with Sox8, further supporting their combinatorial role in activation of Sox10E1 in the trunk neural crest. The results reveal important tissue-specific inputs into Sox10 expression in the developing embryo.", "date": "2017-02-01", "date_type": "published", "publication": "Developmental Biology", "volume": "422", "number": "1", "publisher": "Elsevier", "pagerange": "47-57", "id_number": "CaltechAUTHORS:20170103-114805593", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170103-114805593", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Shurl and Kay Curci Foundation" }, { "agency": "FWF Der Wissenschaftsfonds", "grant_number": "J3538-B19" } ] }, "doi": "10.1016/j.ydbio.2016.12.004", "pmcid": "PMC5810587", "primary_object": { "basename": "nihms939439.pdf", "url": "https://authors.library.caltech.edu/records/p20yt-8zt50/files/nihms939439.pdf" }, "related_objects": [ { "basename": "mmc1.docx", "url": "https://authors.library.caltech.edu/records/p20yt-8zt50/files/mmc1.docx" } ], "resource_type": "article", "pub_year": "2017", "author_list": "Murko, Christina and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1k0cr-jq026", "eprint_id": 72674, "eprint_status": "archive", "datestamp": "2023-08-22 19:16:30", "lastmod": "2023-10-23 22:50:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "cMyc Regulates the Size of the Premigratory Neural Crest Stem Cell Pool", "ispublished": "pub", "full_text_status": "public", "keywords": "MYC; cMyc; neural crest stem cells; neural crest stem cell pool; Miz1; self-renewal; cell survival; neurocristopathies; chicken embryo; crestospheres; cyclin dependent kinase inhibitor; CDKI", "note": "\u00a9 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). \n\nThis work was funded by NIH grants HD037105 and DE024157 (to M.E.B.) and by fellowships from The Sigrid Juselius Foundation, Finnish Cultural Foundation, Jane and Aatos Erkko Foundation, V\u00e4re Foundation, and Ella and Georg Ehrnrooth Foundation (to L.K.). We thank Drs. Crystal Rogers, Stephen Green, and Michael Piacentino and the entire M.E.B. lab members for technical advice. \n\nAuthor Contributions: L.K. and M.E.B. conceived and designed the experimental approach. L.K. performed the experiments and analyzed the data. L.K. and M.E.B. wrote the manuscript.\n\nPublished - 1-s2.0-S2211124716315832-main.pdf
Accepted Version - nihms923632.pdf
Supplemental Material - mmc1.pdf
", "abstract": "The neural crest is a transient embryonic population that originates within the central nervous system (CNS) and then migrates into the periphery and differentiates into multiple cell types. The mechanisms that govern neural crest stem-like characteristics and self-renewal ability are poorly understood. Here, we show that the proto-oncogene cMyc is a critical factor in the chick dorsal neural tube, where it regulates the size of the premigratory neural crest stem cell pool. Loss of cMyc dramatically decreases the number of emigrating neural crest cells due to reduced self-renewal capacity, increased cell death, and shorter duration of the emigration process. Interestingly, rather than via E-Box binding, cMyc acts in the dorsal neural tube by interacting with another transcription factor, Miz1, to promote self-renewal. The finding that cMyc operates in a non-canonical manner in the premigratory neural crest highlights the importance of examining its role at specific time points and in an in vivo context.", "date": "2016-12-06", "date_type": "published", "publication": "Cell Reports", "volume": "17", "number": "10", "publisher": "Cell Press", "pagerange": "2648-2659", "id_number": "CaltechAUTHORS:20161208-151805707", "issn": "2211-1247", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20161208-151805707", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "Sigrid Juselius Foundation" }, { "agency": "Finnish Cultural Foundation" }, { "agency": "Jane and Aatos Erkko Foundation" }, { "agency": "V\u00e4re Foundation" }, { "agency": "Ella and Georg Ehrnrooth Foundation" } ] }, "doi": "10.1016/j.celrep.2016.11.025", "pmcid": "PMC5726515", "primary_object": { "basename": "1-s2.0-S2211124716315832-main.pdf", "url": "https://authors.library.caltech.edu/records/1k0cr-jq026/files/1-s2.0-S2211124716315832-main.pdf" }, "related_objects": [ { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/1k0cr-jq026/files/mmc1.pdf" }, { "basename": "nihms923632.pdf", "url": "https://authors.library.caltech.edu/records/1k0cr-jq026/files/nihms923632.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Kerosuo, Laura and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qacw1-pcp42", "eprint_id": 75598, "eprint_status": "archive", "datestamp": "2023-08-19 00:19:44", "lastmod": "2023-10-25 15:10:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kunttas-Tatli-E", "name": { "family": "Kunttas-Tatli", "given": "E." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "M. E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Role of Pumilio proteins during neural crest development", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 American Society for Cell Biology.\n\nPublished - Kunttas\u2010Tatli_pP2375.pdf
", "abstract": "The neural crest (NC) is a multipotent stem cell\u2010like population, unique to vertebrates, that is characterized by its migratory behavior and broad ability to differentiate into many diverse derivatives including elements of the cardiovascular system, bone and cartilage of the face, the \nperipheral nervous system, and melanocytes. After neurulation, neural crest cells (NCC) delaminate, undergo EMT from the neural tube, and migrate both individually and collectively as chains. Various developmental diseases, including craniofacial abnormalities and neural crest\u2010derived cancers such as melanoma arise due to improper development of NC. While there has been much focus on \ntranscriptional mechanisms in regulation of neural crest specification, the process of cell migration involves rapid changes that likely require post\u2010transcriptional regulation. In order to uncover novel proteins that might influence NC development, we have performed transcriptional \nprofiling of migrating neural crest cells and found >300 genes that are upregulated in the migrating crest including the sequence specific RNA binding protein Pumilio1 (PUM1). PUM proteins are evolutionarily conserved translational regulators that play essential roles during germline development in both invertebrates and vertebrates. Here, we showed that pum1 and pum2 mRNA is present in both premigratory and migratory NC. Pum loss of function resulted in depletion of NC cells migrating neural tube. Conversely, over expression led to an increase in numbers of migrating cells. This led us to think about the potential role of PUM proteins in modulating the specification of NC cells. To identify potential NC targets of PUM, we carried out a bioinformatics screen focusing on NC relevant genes across multiple species that possessed a \nPumilio Response Element (PRE) in their 3'UTR region. The PRE element, 5'\u2010UGUANAUA\u20103,' is a highly conserved consensus that PUM proteins recognize in the 3'UTRs of their targets. Interestingly, several neural crest markers possess a PRE, thus representing potential targets regulated by Pumilio during NC development. Investigation of\nthe specific mechanism whereby PUM proteins regulate NC development is currently in progress.", "date": "2016-12", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "27", "publisher": "American Society for Cell Biology", "pagerange": "Art. No. P2375", "id_number": "CaltechAUTHORS:20170331-134437189", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170331-134437189", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "Kunttas\u2010Tatli_pP2375.pdf", "url": "https://authors.library.caltech.edu/records/qacw1-pcp42/files/Kunttas\u2010Tatli_pP2375.pdf" }, "resource_type": "article", "pub_year": "2016", "author_list": "Kunttas-Tatli, E. and Bronner, M. E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/h9wbb-c9s68", "eprint_id": 75601, "eprint_status": "archive", "datestamp": "2023-08-19 00:19:58", "lastmod": "2023-10-25 15:10:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rogers-Crystal-D", "name": { "family": "Rogers", "given": "C. D." }, "orcid": "0000-0002-9549-1089" }, { "id": "Smith-L-Sorrells", "name": { "family": "Smith", "given": "L. Sorrells" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "M. E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The balance between N-cadherin and E-cadherin orchestrates major neuroectodermal cell fate choices", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 American Society for Cell Biology.\n\nPublished - Rogers_2016pP762.pdf
", "abstract": "Numerous cadherin proteins, including N\u2010cadherin (Ncad), E\u2010cadherin (Ecad), Cadherin\u201011 (Cad11) and Cadherin\u20107 (Cad7), are expressed in the developing neural plate as well as in neural crest cells as they delaminate from the newly closed neural tube. To clarify whether these proteins function \nindependently or coordinately during development, we examined their relative expression in the cranial region of chick embryos. The results revealed surprising overlap of Ecad, Ncad and Cad7 in the neural tube, suggesting possible heterotypic interactions. Using a proximity ligation assay and co\u2010immunoprecipitation to test this hypothesis, we found that Ncad formed heterophilic complexes in the developing neural tube with Ecad. We also determined that modulation of either Ncad or Ecad levels led to reciprocal gain or reduction of the other cadherin protein. Altering levels of the two cadherin proteins affected the early fate specification of ectodermal derivatives, forcing an \naberrant choice between neural crest and epidermal cells. Finally, we identified that the availability of \u03b2\u2010catenin plays a critical role in maintaining the balance between Ncad and Ecad in early development since co\u2010expression of activated \u03b2\u2010catenin rescues the Ncad\u2010overexpression \nphenotype. These results suggest that \u03b2\u2010catenin\u2010mediated balance of Ncad and Ecad proteins is critical for the normal development of the three ectodermal derivatives.", "date": "2016-12", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "27", "publisher": "American Society for Cell Biology", "pagerange": "Art. No. P762", "id_number": "CaltechAUTHORS:20170331-135711455", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170331-135711455", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "Rogers_2016pP762.pdf", "url": "https://authors.library.caltech.edu/records/h9wbb-c9s68/files/Rogers_2016pP762.pdf" }, "resource_type": "article", "pub_year": "2016", "author_list": "Rogers, C. D.; Smith, L. Sorrells; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4769k-9ap87", "eprint_id": 74058, "eprint_status": "archive", "datestamp": "2023-08-20 14:16:02", "lastmod": "2023-10-24 22:03:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Suarez-Bregua-P", "name": { "family": "Suarez-Bregua", "given": "Paula" } }, { "id": "Torres-Nu\u00f1ez-E", "name": { "family": "Torres-Nu\u00f1ez", "given": "Eva" } }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Guerreiro-P", "name": { "family": "Guerreiro", "given": "Pedro" } }, { "id": "Braasch-I", "name": { "family": "Braasch", "given": "Ingo" }, "orcid": "0000-0003-4766-611X" }, { "id": "Prober-D-A", "name": { "family": "Prober", "given": "David A." }, "orcid": "0000-0002-7371-4675" }, { "id": "Moran-P", "name": { "family": "Moran", "given": "Paloma" }, "orcid": "0000-0002-3644-8507" }, { "id": "Cerda-Reverter-J-M", "name": { "family": "Cerda-Reverter", "given": "Jose Miguel" } }, { "id": "Du-Shao-Jun", "name": { "family": "Du", "given": "Shao Jun" } }, { "id": "Adrio-F", "name": { "family": "Adrio", "given": "Fatima" } }, { "id": "Power-D-M", "name": { "family": "Power", "given": "Deborah M." } }, { "id": "Canario-A-V-M", "name": { "family": "Canario", "given": "Adelino V. M." } }, { "id": "Postlethwait-J-H", "name": { "family": "Postlethwait", "given": "John H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Ca\u00f1estro-C", "name": { "family": "Ca\u00f1estro", "given": "Cristian" } }, { "id": "Rotllant-J", "name": { "family": "Rotllant", "given": "Josep" } } ] }, "title": "Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway", "ispublished": "pub", "full_text_status": "public", "keywords": "runx \u2022 phosphate \u2022 calcium \u2022 hypothalamus \u2022 fgf23", "note": "\u00a9 2017 FASEB. \n\nReceived July 27, 2016. Accepted October 11, 2016. Published online before print October 24, 2016. \n\nThe authors thank Yi-lin Yan (University of Oregon) for providing the tip39 (PTH2) expression vector; Prof. N. Lawson (University of Massachusetts Medical School, Worcester, MA, USA) and Prof. K. Kawakami (National Institute of Genetics, Mishima, Japan) for providing the iTol2 constructs; D.A.P. for the hcrt:tdTomato line and Prof. K. M. Kwan (University Of Utah, Salt Lake City, UT, USA) for the bactin2:H2A-mCherry line; In\u00e9s Pazos and Jes\u00fas M\u00e9ndez [Scientific and Technological Research Assistance Centre (CACTI), University of Vigo] for their advice and assistance with the confocal microscope; Rub\u00b4en Chamorro and Rosa Ceinos (IIM\u2013CSIC) for their help in handling and care of the fish and David Guede and Jos\u00e9 R. Caeiro (University of Santiago de Compostela, Santiago, Spain)\nfor their advice and assistance with the micro-CT SkyScan. This\nwork was funded by the Spanish Economy and Competitiveness\nMinistry Project ALG2011-23581 and AGL2014-52473R (to J.R.);\nthe Portuguese Foundation for Science and Technology (Project\nPTDC/BIA-ANM/4225/2012-phos-fate; to P.G.); U. S. National\nInstitutes of Health/Office of the Director Grant R01OD011116\n(alias R01 RR020833; to J.H.P.); Generalitat de Catalunya (Grant\nSGR2014-290) and the Spanish Economy and Competitiveness\nMinistry (Project BFU2010-14875; to C.C.). Partial funding was obtained from Science and Innovation Ministry (AGL2010-22247-C03-01; to J.M.C.-R.), a Campus do Mar Ph.D. grant, and Xunta de Galicia (Santiago, Spain; Project AGL2014-52473R) (to P.S.B.). \n\nJ. Rotllant and P. Suarez-Bregua conceived, designed, and coordinated the study; C. Ca\u00f1estro, I. Braasch, and J. H. Postlethwait performed comparative genomics analyses; P. Suarez-Bregua and E. Torres-Nu\u00f1ez performed promoter and mutation analysis; J. Rotllant performed transgenic lines creation and screening with support from P. Suarez-Bregua and E. Torres-Nu\u00f1ez; J. Cerda-Reverter and P. Suarez-Bregua performed receptor binding studies; P. Suarez-Bregua, A. Saxena, and M. E. Bronner performed laser ablation studies; P. Moran, D. A. Prober, P. Guerreiro,D. M. Power, S. J.Du, F. Adrio, D. M. Power, and A. V. M. Canario provided technical support and contributed to methodology; and P. Suarez-Bregua,\nC. Ca\u00f1estro, and J. Rotllant wrote and revised the manuscript with input from A. Saxena, J. H. Postlethwait, and M. E. Bronner.\n\nSupplemental Material - Supplemental_Figure.docx
Supplemental Material - Supplemental_Figures1.docx
Supplemental Material - Supplemental_Figures2.docx
Supplemental Material - Supplemental_Tables1.docx
", "abstract": "Regulation of bone development, growth, and remodeling traditionally has been thought to depend on endocrine and autocrine/paracrine modulators. Recently, however, brain-derived signals have emerged as key regulators of bone metabolism, although their mechanisms of action have been poorly understood. We reveal the existence of an ancient parathyroid hormone (Pth)4 in zebrafish that was secondarily lost in the eutherian mammals' lineage, including humans, and that is specifically expressed in neurons of the hypothalamus and appears to be a central neural regulator of bone development and mineral homeostasis. Transgenic fish lines enabled mapping of axonal projections leading from the hypothalamus to the brainstem and spinal cord. Targeted laser ablation demonstrated an essential role for of pth4-expressing neurons in larval bone mineralization. Moreover, we show that Runx2 is a direct regulator of pth4 expression and that Pth4 can activate cAMP signaling mediated by Pth receptors. Finally, gain-of-function experiments show that Pth4 can alter calcium/phosphorus levels and affect expression of genes involved in phosphate homeostasis. Based on our discovery and characterization of Pth4, we propose a model for evolution of bone homeostasis in the context of the vertebrate transition from an aquatic to a terrestrial lifestyle.", "date": "2016-10-24", "date_type": "published", "publication": "FASEB Journal", "volume": "31", "number": "2", "publisher": "Federation of American Societies for Experimental Biology", "pagerange": "569-583", "id_number": "CaltechAUTHORS:20170206-080130616", "issn": "0892-6638", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170206-080130616", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Ministerio de Econom\u00eda y Competitividad (MINECO)", "grant_number": "ALG2011-23581" }, { "agency": "Ministerio de Econom\u00eda y Competitividad (MINECO)", "grant_number": "AGL2014-52473R" }, { "agency": "Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT)", "grant_number": "PTDC/BIA-ANM/4225/2012-phos-fate" }, { "agency": "NIH", "grant_number": "R01OD011116" }, { "agency": "NIH", "grant_number": "R01 RR020833" }, { "agency": "Generalitat de Catalunya", "grant_number": "SGR2014-290" }, { "agency": "Ministerio de Econom\u00eda y Competitividad (MINECO)", "grant_number": "BFU2010-14875" }, { "agency": "Ministerio de Econom\u00eda y Competitividad (MINECO)", "grant_number": "AGL2010-22247- C03-01" }, { "agency": "Campus do Mar" }, { "agency": "Xunta de Galicia", "grant_number": "AGL2014-52473R" } ] }, "doi": "10.1096/fj.201600815R", "pmcid": "PMC5240660", "primary_object": { "basename": "Supplemental_Figure.docx", "url": "https://authors.library.caltech.edu/records/4769k-9ap87/files/Supplemental_Figure.docx" }, "related_objects": [ { "basename": "Supplemental_Figures1.docx", "url": "https://authors.library.caltech.edu/records/4769k-9ap87/files/Supplemental_Figures1.docx" }, { "basename": "Supplemental_Figures2.docx", "url": "https://authors.library.caltech.edu/records/4769k-9ap87/files/Supplemental_Figures2.docx" }, { "basename": "Supplemental_Tables1.docx", "url": "https://authors.library.caltech.edu/records/4769k-9ap87/files/Supplemental_Tables1.docx" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Suarez-Bregua, Paula; Torres-Nu\u00f1ez, Eva; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vkz13-k5c15", "eprint_id": 71191, "eprint_status": "archive", "datestamp": "2023-08-20 14:12:28", "lastmod": "2023-10-23 15:28:05", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Tai-Andrew", "name": { "family": "Tai", "given": "Andrew" } }, { "id": "Cheung-Martin", "name": { "family": "Cheung", "given": "Martin" } }, { "id": "Huang-Yong-Heng", "name": { "family": "Huang", "given": "Yong-Heng" } }, { "id": "Jauch-R", "name": { "family": "Jauch", "given": "Ralf" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Cheah-K-S-E", "name": { "family": "Cheah", "given": "Kathryn S. E." } } ] }, "title": "SOXE neofunctionalization and elaboration of the neural crest during chordate evolution", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ \n\nReceived: 20 June 2016. Accepted: 15 September 2016. Published online: 13 October 2016. \n\nThis work was supported by grants from the Research Grants Council and University Grants Council of Hong Kong (7337/01M), (AoE/M-04/04), (T12-708/12-N) and (T12C-714/14-R) to K.S.E.C., GRF_17110715 to M.C and NS086907 to M.E.B. We thank Robin Lovell-Badge and Patrick Tam for helpful discussion, and Hisato Kondoh and Masanori Uchikawa for providing the Sox2-NC1 construct. We thank Yogesh Srivastava (GIBH) for help with structural modeling and May Cheung for technical assistance. R.J. is supported by a 2013 MOST China-EU Science and Technology Cooperation Program (grant number 2013DFE33080), by the National Natural Science Foundation of China (grant number 31471238), a 100 talent award of the Chinese Academy of Sciences and a Science and Technology Planning Project of Guangdong Province, China (2014B030301058). \n\nAndrew Tai & Martin Cheung: These authors contributed equally to this work. \n\nAuthor Contributions: A.T. and M.C. designed and performed experiments, analyzed data, and wrote the manuscript. Y.-H.H. designed and performed experiments. R.J. designed experiments, analyzed data and wrote the manuscript. M.E.B. and K.S.E.C. designed experiments, analyzed data and wrote the manuscript. \n\nThe authors declare no competing financial interests. \n\nAccession codes: Accession numbers for the sequences used in this study are as follows: Rattus norvegicus (Sox8: NP_001100458; Sox10: NP_); Mus musculus (Sox8: NP_035577; Sox9: NM_035578; Sox10: NP_035567); Gallus gallus (Sox8: NP_990062; Sox9: NP_989612; Sox10: NP_990123); Homo sapiens (Sox8: NM_055402; Sox9: NP_000337; Sox10: NP_990123); Xenopus laevis (Sox8: NP_001083964; Sox9: NP_001084276; Sox10: NP_001082358); Danio rerio (Sox8: NP_001020636; Sox9a: NP_571718; Sox9b: AAH67133; Sox10: NP_571950); Petromyzon marinus (SoxE1: AAW34332; SoxE2: ABC58684; SoxE3: ABC58685); Eptatretus burgeri (Sox9: BAG11536); Ciona intestinalis (SoxE: CAD58841); Lytechinus variegatus (SoxE: ABY40629); Drosophila melanogaster (Sox100B: NP_651839); Apis mellifera (SoxE1: XP_001122993; SoxE2: XP_001122996) and Nasonia vitripennis (SoxE1: XP_001604913; SoxE2: XP_008213434).\n\nPublished - srep34964.pdf
Supplemental Material - srep34964-s1.pdf
", "abstract": "During chordate evolution, two genome-wide duplications facilitated acquisition of vertebrate traits, including emergence of neural crest cells (NCCs), in which neofunctionalization of the duplicated genes are thought to have facilitated development of craniofacial structures and the peripheral nervous system. How these duplicated genes evolve and acquire the ability to specify NC and their derivatives are largely unknown. Vertebrate SoxE paralogues, most notably Sox9/10, are essential for NC induction, delamination and lineage specification. In contrast, the basal chordate, amphioxus, has a single SoxE gene and lacks NC-like cells. Here, we test the hypothesis that duplication and divergence of an ancestral SoxE gene may have facilitated elaboration of NC lineages. By using an in vivo expression assay to compare effects of AmphiSoxE and vertebrate Sox9 on NC development, we demonstrate that all SOXE proteins possess similar DNA binding and homodimerization properties and can induce NCCs. However, AmphiSOXE is less efficient than SOX9 in transactivation activity and in the ability to preferentially promote glial over neuronal fate, a difference that lies within the combined properties of amino terminal and transactivation domains. We propose that acquisition of AmphiSoxE expression in the neural plate border led to NCC emergence while duplication and divergence produced advantageous mutations in vertebrate homologues, promoting elaboration of NC traits.", "date": "2016-10-13", "date_type": "published", "publication": "Scientific Reports", "volume": "6", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 34964", "id_number": "CaltechAUTHORS:20161017-151104599", "issn": "2045-2322", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20161017-151104599", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Research Grants Council of Hong Kong", "grant_number": "7337/01M" }, { "agency": "Research Grants Council of Hong Kong", "grant_number": "AoE/M-04/04" }, { "agency": "Research Grants Council of Hong Kong", "grant_number": "T12-708/12-N" }, { "agency": "Research Grants Council of Hong Kong", "grant_number": "T12C-714/14-R" }, { "agency": "NIH Graduate Fellowship", "grant_number": "GRF_17110715" }, { "agency": "NIH", "grant_number": "NS086907" }, { "agency": "Ministry of Science and Technology (China)", "grant_number": "2013DFE33080" }, { "agency": "National Natural Science Foundation of China", "grant_number": "31471238" }, { "agency": "100-Talent Project of Chinese Academy of Sciences" }, { "agency": "Guangdong Province", "grant_number": "2014B030301058" } ] }, "doi": "10.1038/srep34964", "pmcid": "PMC5062122", "primary_object": { "basename": "srep34964-s1.pdf", "url": "https://authors.library.caltech.edu/records/vkz13-k5c15/files/srep34964-s1.pdf" }, "related_objects": [ { "basename": "srep34964.pdf", "url": "https://authors.library.caltech.edu/records/vkz13-k5c15/files/srep34964.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Tai, Andrew; Cheung, Martin; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/z27p8-phb40", "eprint_id": 70986, "eprint_status": "archive", "datestamp": "2023-08-22 18:50:54", "lastmod": "2023-10-23 15:14:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Choi-Harry-M-T", "name": { "family": "Choi", "given": "Harry M. T." }, "orcid": "0000-0002-1530-0773" }, { "id": "Calvert-Colby-R", "name": { "family": "Calvert", "given": "Colby R." } }, { "id": "Husain-Naeem", "name": { "family": "Husain", "given": "Naeem" }, "orcid": "0000-0003-4962-7237" }, { "id": "Barsi-Julius-C", "name": { "family": "Barsi", "given": "Julius C." }, "orcid": "0000-0002-5161-6708" }, { "id": "Deverman-Benjamin-E", "name": { "family": "Deverman", "given": "Benjamin E." }, "orcid": "0000-0002-6223-9303" }, { "id": "Hunter-Ryan-C", "name": { "family": "Hunter", "given": "Ryan C." }, "orcid": "0000-0003-3841-1676" }, { "id": "Kato-Mihoko", "name": { "family": "Kato", "given": "Mihoko" }, "orcid": "0000-0003-3827-8879" }, { "id": "Lee-S-Melanie", "name": { "family": "Lee", "given": "S. Melanie" } }, { "id": "Abelin-Anna-C-T", "name": { "family": "Abelin", "given": "Anna C. T." } }, { "id": "Rosenthal-Adam-Z", "name": { "family": "Rosenthal", "given": "Adam Z." }, "orcid": "0000-0002-6936-3665" }, { "id": "Akbari-Omar-S", "name": { "family": "Akbari", "given": "Omar S." }, "orcid": "0000-0002-6853-9884" }, { "id": "Li-Yuwei", "name": { "family": "Li", "given": "Yuwei" }, "orcid": "0000-0001-7753-4869" }, { "id": "Hay-B-A", "name": { "family": "Hay", "given": "Bruce A." }, "orcid": "0000-0002-5486-0482" }, { "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "orcid": "0000-0002-7699-0173" }, { "id": "Patterson-P-H", "name": { "family": "Patterson", "given": "Paul H." } }, { "id": "Davidson-E-H", "name": { "family": "Davidson", "given": "Eric H." } }, { "id": "Mazmanian-S-K", "name": { "family": "Mazmanian", "given": "Sarkis K." }, "orcid": "0000-0003-2713-1513" }, { "id": "Prober-D-A", "name": { "family": "Prober", "given": "David A." }, "orcid": "0000-0002-7371-4675" }, { "id": "Leadbetter-J-R", "name": { "family": "Leadbetter", "given": "Jared R." }, "orcid": "0000-0002-7033-0844" }, { "id": "Newman-D-K", "name": { "family": "Newman", "given": "Dianne K." }, "orcid": "0000-0003-1647-1918" }, { "id": "Readhead-Carol", "name": { "family": "Readhead", "given": "Carol" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Wold-B-J", "name": { "family": "Wold", "given": "Barbara" }, "orcid": "0000-0003-3235-8130" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Pierce-N-A", "name": { "family": "Pierce", "given": "Niles A." }, "orcid": "0000-0003-2367-4406" } ] }, "title": "Mapping a multiplexed zoo of mRNA expression", "ispublished": "pub", "full_text_status": "public", "keywords": "In situ hybridization, In situ amplification, Hybridization chain reaction (HCR), Multiplexing, Deep sample penetration, High contrast, Subcellular resolution, Bacteria, Whole-mount embryos and larvae, Tissue sections", "note": "\u00a9 2016. Published by The Company of Biologists Ltd. \n\nReceived May 20, 2016. Accepted August 1, 2016.\n\nThis work was funded by the National Institutes of Health (NIH) [5R01EB006192]; the National Science Foundation Molecular Programming Project [NSF-CCF-1317694]; the Gordon and Betty Moore Foundation [GBMF2809]; the Beckman Institute at Caltech (PMTC); the Translational Biomedical Imaging Laboratory at CHLA; the Translational Imaging Center at USC; a Christensen Fellowship at St Catherine's College, University of Oxford; and by the John Simon Guggenheim Memorial Foundation. Deposited in PMC for release after 12 months. \n\nCompeting interests: The authors declare competing financial interests in the form of patents and pending patent applications. \n\nAuthor contributions: Study conceived by N.A.P. in consultation with M.E.B., E.H.D., S.E.F., B.A.H., J.R.L., D.K.N., P.H.P., M.v.d.R., B.W. Experiments designed by H.M.T.C. and N.A.P. Preliminary studies and protocol adaptation performed by S.M.L., R.C.H., A.Z.R., H.M.T.C. (bacteria), C.R.C. (nematode), N.H. (fly), J.C.B., C.R.C. (urchin), H.M.T.C. (zebrafish), T.S.-S., C.R.C. (chicken), A.C.T.A., B.E.D., D.H., H.M.T.C. (mouse), and H.M.T.C., N.H. (human), in consultation with D.K.N., J.R.L. (bacteria), M.K. (nematode), R.L., S.E.F. (chicken), R.L., C.R.C., S.E.F., B.W. (mouse), M.v.d.R. (human), and H.M.T.C., N.A.P. (all organisms). Final protocols optimized and final data collected by H.M.T.C. (bacteria, zebrafish, mouse), C.R.C. (nematode, urchin, chicken) and N.H. (fly, human). Final data analyzed by: S.M.L., S.K.M., R.C.H., D.K.N., A.Z.R., J.R.L. (bacteria), M.K., P.S., C.R.C. (nematode), O.S.A., B.A.H., N.H. (fly), J.C.B., C.R.C. (urchin), D.A.P., S.E.F. (fish), T.S.-S., M.E.B., C.R.C. (chicken), A.C.T.A., B.W., B.E.D., D.H., Y.L., C.R., R.L., S.E.F. (mouse), A.C.M., M.v.d.R., N.H. (human) and H.M.T.C and N.A.P. (all organisms). Paper and supplementary information written by H.M.T.C. and N.A.P. Paper was edited and approved by all coauthors.\n\nPublished - 3632.full.pdf
Supplemental Material - DEV140137supp.pdf
", "abstract": "In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs are mapped in a single sample, are essential for studying regulatory interactions, but remain cumbersome in most model organisms. Programmable in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) overcome this longstanding challenge by operating independently within a sample, enabling multiplexed experiments to be performed with an experimental timeline independent of the number of target mRNAs. To assist biologists working across a broad spectrum of organisms, we demonstrate multiplexed in situ HCR in diverse imaging settings: bacteria, whole-mount nematode larvae, whole-mount fruit fly embryos, whole-mount sea urchin embryos, whole-mount zebrafish larvae, whole-mount chicken embryos, whole-mount mouse embryos and formalin-fixed paraffin-embedded human tissue sections. In addition to straightforward multiplexing, in situ HCR enables deep sample penetration, high contrast and subcellular resolution, providing an incisive tool for the study of interlaced and overlapping expression patterns, with implications for research communities across the biological sciences.", "date": "2016-10-01", "date_type": "published", "publication": "Development", "volume": "143", "number": "19", "publisher": "Company of Biologists", "pagerange": "3632-3637", "id_number": "CaltechAUTHORS:20161011-070233463", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20161011-070233463", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5R01EB006192" }, { "agency": "NSF", "grant_number": "CCF-1317694" }, { "agency": "Gordon and Betty Moore Foundation", "grant_number": "GBMF2809" }, { "agency": "Caltech Beckman Institute" }, { "agency": "CHLA Translational Biomedical Imaging Laboratory" }, { "agency": "USC Translational Imaging Center" }, { "agency": "St. Catharine's College" }, { "agency": "University of Oxford" }, { "agency": "John Simon Guggenheim Memorial Foundation" } ] }, "local_group": { "items": [ { "id": "Division-of-Geological-and-Planetary-Sciences" } ] }, "doi": "10.1242/dev.140137", "pmcid": "PMC5087610", "primary_object": { "basename": "3632.full.pdf", "url": "https://authors.library.caltech.edu/records/z27p8-phb40/files/3632.full.pdf" }, "related_objects": [ { "basename": "DEV140137supp.pdf", "url": "https://authors.library.caltech.edu/records/z27p8-phb40/files/DEV140137supp.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Choi, Harry M. T.; Calvert, Colby R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1t6a4-18f49", "eprint_id": 68664, "eprint_status": "archive", "datestamp": "2023-08-20 12:28:20", "lastmod": "2023-10-19 22:25:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Reprogramming of avian neural crest axial identity and cell fate", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 American Association for the Advancement of Science. \n\nReceived 18 January 2016; accepted 23 May 2016. \n\nWe thank J. Tan-Cabugao, M. Stone, B. Jun, and D. S. E. Koo for technical assistance. The Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory provided sequencing and bioinformatics support. We are indebted to D. Perez, K. Beadle, and R. Diamond for cell-sorting assistance at the Caltech Flow Cytometry Cell Sorting Facility. We also thank M. Barembaum for the Sox8 and Tfap2b expression constructs. This work was supported by NIH grants DE024157 and HD037105 to M.E.B. M.S.-C. was funded by a fellowship from the Pew Fellows Program in Biomedical Sciences and by NIH grant K99DE024232. The supplementary materials contain additional data.\n\nAccepted Version - nihms825478.pdf
Supplemental Material - aaf2729-Simoes-Costa-SM.pdf
Supplemental Material - aaf2729-Simoes-Costa-SM.table.S1.xlsx
", "abstract": "Neural crest populations along the embryonic body axis of vertebrates differ in developmental potential and fate, so that only the cranial neural crest can contribute to the craniofacial skeleton in vivo. We explored the regulatory program that imbues the cranial crest with its specialized features. Using axial-level specific enhancers to isolate and perform genome-wide profiling of the cranial versus trunk neural crest in chick embryos, we identified and characterized regulatory relationships between a set of cranial-specific transcription factors. Introducing components of this circuit into neural crest cells of the trunk alters their identity and endows these cells with the ability to give rise to chondroblasts in vivo. Our results demonstrate that gene regulatory circuits that support the formation of particular neural crest derivatives may be used to reprogram specific neural crest\u2013derived cell types.", "date": "2016-06-24", "date_type": "published", "publication": "Science", "volume": "352", "number": "6293", "publisher": "American Association for the Advancement of Science", "pagerange": "1570-1573", "id_number": "CaltechAUTHORS:20160624-140845423", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160624-140845423", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Pew Foundation" }, { "agency": "NIH", "grant_number": "K99DE024232" } ] }, "doi": "10.1126/science.aaf2729", "pmcid": "PMC5100669", "primary_object": { "basename": "aaf2729-Simoes-Costa-SM.pdf", "url": "https://authors.library.caltech.edu/records/1t6a4-18f49/files/aaf2729-Simoes-Costa-SM.pdf" }, "related_objects": [ { "basename": "aaf2729-Simoes-Costa-SM.table.S1.xlsx", "url": "https://authors.library.caltech.edu/records/1t6a4-18f49/files/aaf2729-Simoes-Costa-SM.table.S1.xlsx" }, { "basename": "nihms825478.pdf", "url": "https://authors.library.caltech.edu/records/1t6a4-18f49/files/nihms825478.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Sim\u00f5es-Costa, Marcos and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/664na-xdj96", "eprint_id": 67845, "eprint_status": "archive", "datestamp": "2023-08-20 12:05:31", "lastmod": "2023-10-18 21:48:26", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "How inhibitory cues can both constrain and promote cell migration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 Bronner. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unpor ted license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nSubmitted: 19 May 2016; Accepted: 20 May 2016. \n\nI thank Paige O'Connell for drawing Fig. 1. Work in the Bronner laboratory is supported by National Institutes of Health grants DE024157 and HD037105. \n\nThe author declares no conflicting financial interests.\n\nPublished - J_Cell_Biol-2016-Bronner-505-7.pdf
", "abstract": "Collective cell migration is a common feature in both embryogenesis and metastasis. By coupling studies of neural crest migration in vivo and in vitro with mathematical modeling, Szab\u00f3 et al. (2016, J. Cell Biol., http://dx.doi.org/10.1083/jcb.201602083) demonstrate that the proteoglycan versican forms a physical boundary that constrains neural crest cells to discrete streams, in turn facilitating their migration.", "date": "2016-06-06", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "213", "number": "5", "publisher": "Rockefeller University Press", "pagerange": "505-507", "id_number": "CaltechAUTHORS:20160610-133948679", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160610-133948679", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1083/jcb.201605074", "pmcid": "PMC4896061", "primary_object": { "basename": "J_Cell_Biol-2016-Bronner-505-7.pdf", "url": "https://authors.library.caltech.edu/records/664na-xdj96/files/J_Cell_Biol-2016-Bronner-505-7.pdf" }, "resource_type": "article", "pub_year": "2016", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sjqz2-sze06", "eprint_id": 65903, "eprint_status": "archive", "datestamp": "2023-08-22 18:02:57", "lastmod": "2023-10-18 16:55:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-R", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" } ] }, "title": "The vertebrate Hox gene regulatory network for hindbrain segmentation: Evolution and diversification", "ispublished": "pub", "full_text_status": "restricted", "keywords": "chordates; gene regulatory networks; hindbrain; Hox genes; rhombomeres; segmentation; vertebrate evolution", "note": "\u00a9 2016 Wiley Periodicals, Inc. \n\nArticle first published online: 29 MAR 2016. \n\nWe wish to thank members of the Krumlauf and Bronner labs for discussions, Mark Miller for illustrations, and the Stowers Institute for supporting HP and RK. \n\nThe authors have declared no conflicts of interest.", "abstract": "Hindbrain development is orchestrated by a vertebrate gene regulatory network that generates segmental patterning along the anterior\u2013posterior axis via Hox genes. Here, we review analyses of vertebrate and invertebrate chordate models that inform upon the evolutionary origin and diversification of this network. Evidence from the sea lamprey reveals that the hindbrain regulatory network generates rhombomeric compartments with segmental Hox expression and an underlying Hox code. We infer that this basal feature was present in ancestral vertebrates and, as an evolutionarily constrained developmental state, is fundamentally important for patterning of the vertebrate hindbrain across diverse lineages. Despite the common ground plan, vertebrates exhibit neuroanatomical diversity in lineage-specific patterns, with different vertebrates revealing variations of Hox expression in the hindbrain that could underlie this diversification. Invertebrate chordates lack hindbrain segmentation but exhibit some conserved aspects of this network, with retinoic acid signaling playing a role in establishing nested domains of Hox expression.", "date": "2016-06", "date_type": "published", "publication": "Bioessays", "volume": "38", "number": "6", "publisher": "Wiley-Blackwell", "pagerange": "526-538", "id_number": "CaltechAUTHORS:20160404-144238233", "issn": "0265-9247", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160404-144238233", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Stowers Institute for Medical Research" } ] }, "doi": "10.1002/bies.201600010", "resource_type": "article", "pub_year": "2016", "author_list": "Parker, Hugo J.; Bronner, Marianne E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/a007w-5wr87", "eprint_id": 73749, "eprint_status": "archive", "datestamp": "2023-08-20 11:31:40", "lastmod": "2023-10-24 16:24:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Figueroa-Raul", "name": { "family": "Figueroa", "given": "Raul" } }, { "id": "Lansford-Rusty-D", "name": { "family": "Lansford", "given": "Rusty" }, "orcid": "0000-0002-2159-3699" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ex Vivo Culture of Lung Buds on the Chorioallantoic Membrane of the Avian Embryo", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 American Thoracic Society.", "abstract": "Rationale:\nDevelopment of the vertebrate lung involves a complex series of interactions between the foregut endoderm and the\nadjacent mesenchyme, resulting in formation of the paired lung buds. The lung buds subsequently give rise to mature lungs through processes which include branching morphogenesis, cell commitment and differentiation. At present it remains unclear what other cell types may play a role in patterning of the developing airways and the generation of diverse cell lineages. To address this problem, we have developed an ex vivo lung culture system which allows us to experimentally manipulate the developing lung buds. The chick embryo lends itself particularly well to such manipulations and It has long served as an important model system for developmental studies. In\naddition, development of the respiratory system in birds has been the subject of numerous descriptive studies which can provide a useful background to more experimental approaches.\n\nMethods:\nWe have explanted the lung buds of developing day 4 chick or quail embryos onto the chorioallantoic membranes of host\nembryos at a similar developmental stage. In some cases, donor king buds were taken from transgenic quail, in which all or a subset of cells were labeled constitutively through expression of fluorescent chimeric protein. The explants were cultured further before fixation and analysis by conventional and confocal laser scanning microscopy.\n\nResults:\nWe have demonstrated that developing lung buds explanted to chorioallantoic membranes continue to develop further, in\nsome cases surviving for a further 6 days of development. In addition, the simple airway of the explanted lung buds undergoes branching morphogenesis to form a complex airway comparable to that found in lungs of similarly-staged un-operated embryos. Moreover, the explanted lung buds develop a capillary network that appears to establish a circulation with that of the chorioallantoic membrane.\n\nConclusions: \nThe chorioallantoic membrane culture system can be used to sustain the growth and development of isolated chick and\nquail lung buds, thus permitting their experimental manipulation to determine the role of different cell populations in lung development.", "date": "2016-05", "date_type": "published", "publication": "American Journal of Respiratory and Critical Care Medicine", "volume": "193", "publisher": "American Thoracic Society", "pagerange": "Art. No. A5945", "id_number": "CaltechAUTHORS:20170126-090551112", "issn": "1073-449X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170126-090551112", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "article", "pub_year": "2016", "author_list": "Selleck, Mark A. J.; Figueroa, Raul; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j0rmz-rr323", "eprint_id": 98705, "eprint_status": "archive", "datestamp": "2023-08-20 11:18:46", "lastmod": "2023-10-18 17:35:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Eric Davidson, friend, colleague and mentor", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2016 Elsevier Inc. \n\nAvailable online 26 January 2016.", "abstract": "My interactions with Eric Davidson started later in his life; first when he invited me to teach in the Embryology Course at the Marine Biological Laboratory and solidifying after I joined the Caltech faculty in 1996. Then in his 50's, Eric was still a motor cycle-riding, football-playing and overall imposing figure who did not suffer fools and said exactly what was on his mind. Needless to say, I was intimidated when I first met him\u2014both by his insightful and piercing intellect and assertive manner. But there was also a gentle and caring side of Eric. He was always a strong supporter of women in science, before it was \"politically correct\" and popular. He loved nothing better than an active and lively exchange of scientific ideas and testing the boundaries of knowledge.", "date": "2016-04-15", "date_type": "published", "publication": "Developmental Biology", "volume": "412", "number": "2", "publisher": "Elsevier", "pagerange": "S31-S32", "id_number": "CaltechAUTHORS:20190918-072800600", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20190918-072800600", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2016.01.023", "resource_type": "article", "pub_year": "2016", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b8g8j-9sv49", "eprint_id": 64164, "eprint_status": "archive", "datestamp": "2023-08-22 17:35:39", "lastmod": "2023-10-17 19:33:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Roellig-Daniela", "name": { "family": "Roellig", "given": "Daniela" }, "orcid": "0000-0002-7558-3592" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The epigenetic modifier DNMT3A is necessary for proper otic placode formation", "ispublished": "pub", "full_text_status": "public", "keywords": "Otic; Placode; DNMT3A; Gbx2; Pax2", "note": "\u00a9 2016 Elsevier Inc. \n\nReceived date: 25 January 2016; Accepted date: 25 January 2016; Available online 28 January 2016. \n\nWe thank Bertrand B\u00e9naz\u00e9raf for helpful discussions and critical reading of the manuscript. This work was funded by grants DC011577 and DE16459 from the NIH. DR was funded by the Deutsche Forschungsgemeinschaft (DFG; RO 4334/1-1). \n\nConflict of Interest statement. None declared.\n\nAccepted Version - 1-s2.0-S0012160616300380-main.pdf
Accepted Version - nihms-757595.pdf
Supplemental Material - mmc1.pdf
", "abstract": "Cranial placodes are thickenings in the ectoderm that give rise to sensory organs and peripheral ganglia of the vertebrate head. At gastrula and neurula stages, placodal precursors are intermingled in the neural plate border with future neural and neural crest cells. Here, we show that the epigenetic modifier, DNA methyl transferase (DNMT) 3A, expressed in the neural plate border region, influences development of the otic placode which will contribute to the ear. DNMT3A is expressed in the presumptive otic region at gastrula through neurula stages and later in the otic placode itself. Whereas neural plate border and non-neural ectoderm markers Erni, Dlx5, Msx1 and Six1 are unaltered, DNMT3A loss of function leads to early reduction in the expression of the key otic placode specifier genes Pax2 and Gbx2 and later otic markers Sox10 and Soho1. Reduction of Gbx2 was first observed at HH7, well before loss of other otic markers. Later, this translates to significant reduction in the size of the otic vesicle. Based on these results, we propose that DNMT3A is important for enabling the activation of Gbx2 expression, necessary for normal development of the inner ear.", "date": "2016-03-15", "date_type": "published", "publication": "Developmental Biology", "volume": "411", "number": "2", "publisher": "Elsevier", "pagerange": "294-300", "id_number": "CaltechAUTHORS:20160202-103243206", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160202-103243206", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DC011577" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "RO 4334/1-1" } ] }, "doi": "10.1016/j.ydbio.2016.01.034", "pmcid": "PMC4783191", "primary_object": { "basename": "1-s2.0-S0012160616300380-main.pdf", "url": "https://authors.library.caltech.edu/records/b8g8j-9sv49/files/1-s2.0-S0012160616300380-main.pdf" }, "related_objects": [ { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/b8g8j-9sv49/files/mmc1.pdf" }, { "basename": "nihms-757595.pdf", "url": "https://authors.library.caltech.edu/records/b8g8j-9sv49/files/nihms-757595.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Roellig, Daniela and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/agev6-vq138", "eprint_id": 64623, "eprint_status": "archive", "datestamp": "2023-08-22 17:27:56", "lastmod": "2023-10-17 21:33:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uribe-Rosa-A", "name": { "family": "Uribe", "given": "Rosa A." }, "orcid": "0000-0002-0427-4493" }, { "id": "Gu-Tiffany", "name": { "family": "Gu", "given": "Tiffany" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system", "ispublished": "pub", "full_text_status": "public", "keywords": "serotonergic neuron; ptf1a; enteric nervous system; zebrafish", "note": "\u00a9 2016 Wiley Periodicals, Inc. \n\nAccepted manuscript online: 10 FEB 2016 10:37PM EST. Manuscript Accepted: 9 FEB 2016. Manuscript Revised: 4 JAN 2016. Manuscript Received: 7 SEP 2015. \n\nWe thank Ryan Anderson (Indiana University School of Medicine) for sharing the Tg(ptf1a:GFP) line and Robert Kelsh (University of Bath) for sharing the Tg(-4725sox10:Cre;elf1a:loxp-GFP-loxp-dsRedpA) line. We thank Crystal Rogers and Stephen Green for helpful discussions, Yuk Fai Leung (Purdue University) for pCS2-ptf1a construct and Martha Henderson and David Mayorga for fish care. Research was supported by grants from NIH DE024157 to M.E.B., from NIH F32 HD080343 to R.A.U. and a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award to R.A.U.\n\nAccepted Version - nihms759765.pdf
", "abstract": "The enteric nervous system, the largest division of the peripheral nervous system, is derived from vagal neural crest cells that invade and populate the entire length of the gut to form diverse neuronal subtypes. Here, we identify a novel population of neurons within the enteric nervous system of zebrafish larvae that express the transgenic marker ptf1a:GFP within the midgut. Genetic lineage analysis reveals that enteric ptf1a:GFP+ cells are derived from the neural crest and that most ptf1a:GFP+ neurons express the neurotransmitter 5HT, demonstrating that they are serotonergic. This transgenic line, Tg(ptf1a:GFP), provides a novel neuronal marker for a subpopulation of neurons within the enteric nervous system, and highlights the possibility that Ptf1a may act as an important transcription factor for enteric neuron development.", "date": "2016-03", "date_type": "published", "publication": "Genesis", "volume": "54", "number": "3", "publisher": "Wiley", "pagerange": "123-128", "id_number": "CaltechAUTHORS:20160222-084508938", "issn": "1526-954X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160222-084508938", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH Postdoctoral Fellowship", "grant_number": "F32 HD080343" }, { "agency": "Burroughs Wellcome Fund" } ] }, "doi": "10.1002/dvg.22927", "pmcid": "PMC4803644", "primary_object": { "basename": "nihms759765.pdf", "url": "https://authors.library.caltech.edu/records/agev6-vq138/files/nihms759765.pdf" }, "resource_type": "article", "pub_year": "2016", "author_list": "Uribe, Rosa A.; Gu, Tiffany; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/s0046-hk089", "eprint_id": 64178, "eprint_status": "archive", "datestamp": "2023-08-20 10:01:14", "lastmod": "2023-10-17 19:34:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Huang-Miller", "name": { "family": "Huang", "given": "Miller" } }, { "id": "Miller-M-L", "name": { "family": "Miller", "given": "Matthew L." } }, { "id": "McHenry-L-K", "name": { "family": "McHenry", "given": "Lauren K." } }, { "id": "Zheng-Tina", "name": { "family": "Zheng", "given": "Tina" } }, { "id": "Zhen-Qiqi", "name": { "family": "Zhen", "given": "Qiqi" } }, { "id": "Ilkhanizadeh-S", "name": { "family": "Ilkhanizadeh", "given": "Shirin" } }, { "id": "Conklin-B-R", "name": { "family": "Conklin", "given": "Bruce R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Weiss-W-A", "name": { "family": "Weiss", "given": "William A." } } ] }, "title": "Generating trunk neural crest from human pluripotent stem cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. \n\nreceived: 17 August 2015. accepted: 17 December 2015. Published: 27 January 2016. \n\nWAW was supported by NIH grants: U01U01CA176287, P30CA82103, R01NS088355, R01CA102321, and by grants from the Alex's Lemonade Stand, Katie Dougherty, Ross K. MacNeill, and Samuel G. Waxman Foundations, and a CureSearch Grand Challenge Award. MH was supported by a Postdoctoral Fellowship, PF-13-295-01\u2013TBG from the American Cancer Society, and an Alex's Lemonade Stand Foundation Young Investigator Award. SI was supported by the Swedish Brain Tumor Foundation and the Swedish Childhood Cancer Foundation. MEB was supported by NIH grant R01DE024157. BRC was supported by NIH grants: U01HL099997, P01HL089707, and R01HL060664. \n\nAuthor Contributions: M.H. designed and performed experiments, analyzed data, and wrote the manuscript. M.L.M., L.K.M., T.Z. and Q.Z. performed experiments and analyzed data. S.I. assisted in statistical analysis and quantification of immunofluorescence. B.R.C. and M.E.B. designed experiments and wrote the manuscript. W.A.W. supervised the study and wrote the manuscript. \n\nThe authors declare no competing financial interests.\n\nPublished - srep19727.pdf
Supplemental Material - srep19727-s1.pdf
", "abstract": "Neural crest cells (NCC) are stem cells that generate different lineages, including neuroendocrine, melanocytic, cartilage, and bone. The differentiation potential of NCC varies according to the level from which cells emerge along the neural tube. For example, only anterior \"cranial\" NCC form craniofacial bone, whereas solely posterior \"trunk\" NCC contribute to sympathoadrenal cells. Importantly, the isolation of human fetal NCC carries ethical and scientific challenges, as NCC induction typically occur before pregnancy is detectable. As a result, current knowledge of NCC biology derives primarily from non-human organisms. Important differences between human and non-human NCC, such as expression of HNK1 in human but not mouse NCC, suggest a need to study human NCC directly. Here, we demonstrate that current protocols to differentiate human pluripotent stem cells (PSC) to NCC are biased toward cranial NCC. Addition of retinoic acid drove trunk-related markers and HOX genes characteristic of a posterior identity. Subsequent treatment with bone morphogenetic proteins (BMPs) enhanced differentiation to sympathoadrenal cells. Our approach provides methodology for detailed studies of human NCC, and clarifies roles for retinoids and BMPs in the differentiation of human PSC to trunk NCC and to sympathoadrenal lineages.", "date": "2016-01-27", "date_type": "published", "publication": "Scientific Reports", "volume": "6", "publisher": "Nature Publishing Group", "pagerange": "Art. No. 19727", "id_number": "CaltechAUTHORS:20160203-070724904", "issn": "2045-2322", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160203-070724904", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "U01U01CA176287" }, { "agency": "NIH", "grant_number": "P30CA82103" }, { "agency": "NIH", "grant_number": "R01NS088355" }, { "agency": "NIH", "grant_number": "R01CA102321" }, { "agency": "Alex's Lemonade Stand Foundation for Childhood Cancer" }, { "agency": "CureSearch Grand Challenge Award" }, { "agency": "American Cancer Society", "grant_number": "PF-13-295-01\u2013TBG" }, { "agency": "Swedish Brain Tumor Foundation" }, { "agency": "Swedish Childhood Cancer Foundation" }, { "agency": "NIH", "grant_number": "R01DE024157" }, { "agency": "NIH", "grant_number": "U01HL099997" }, { "agency": "NIH", "grant_number": "P01HL089707" }, { "agency": "NIH", "grant_number": "R01HL060664" }, { "agency": "Katie Dougherty" }, { "agency": "Samuel G. Waxman Foundation" }, { "agency": "Ross K. MacNeill Foundation" } ] }, "doi": "10.1038/srep19727", "pmcid": "PMC4728437", "primary_object": { "basename": "srep19727-s1.pdf", "url": "https://authors.library.caltech.edu/records/s0046-hk089/files/srep19727-s1.pdf" }, "related_objects": [ { "basename": "srep19727.pdf", "url": "https://authors.library.caltech.edu/records/s0046-hk089/files/srep19727.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Huang, Miller; Miller, Matthew L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9mnc6-fda82", "eprint_id": 67514, "eprint_status": "archive", "datestamp": "2023-08-20 09:35:05", "lastmod": "2023-10-18 21:15:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Mukendi-C", "name": { "family": "Mukendi", "given": "Christian" } }, { "id": "Dean-N", "name": { "family": "Dean", "given": "Nicholas" } }, { "id": "Lala-R", "name": { "family": "Lala", "given": "Rushil" } }, { "id": "Smith-J-J", "name": { "family": "Smith", "given": "Jeramiah J." }, "orcid": "0000-0001-5333-5531" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya V." } } ] }, "title": "Evolution of the vertebrate claudin gene family: insights from a basal vertebrate, the sea lamprey", "ispublished": "pub", "full_text_status": "public", "keywords": "claudin, lamprey, vertebrate primordia, embryo", "note": "\u00a9 2016 UPV/EHU Press. \n\nAccepted: 18 February 2016. \n\nWe thank Tatjana Sauka-Spengler for providing PmCldn3b, PmCldn8b and Pm Cldn19b clones, Thembekile Zwane for assistance with lamprey embryo collection and morpholino injection. \n\nThis work is based on the research supported wholly by the National Research Foundation of South Africa (Grant reference number 80717 to NN and grantholder-linked student funding to CK and RL). The grantholder acknowledges that opinions, findings, conclusions and recommendations expressed in any publication generated by the NRF supported research are those of the authors, and that the NRF accepts no liability whatsoever in this regard.\n\nPublished - ft39.pdf
Supplemental Material - IntJDevBiol-150364nn-SuppMaterial.pdf
", "abstract": "Claudins are major constituents of tight junctions, contributing both to their intercellular sealing and selective permeability properties. While claudins and claudin-like molecules are present in some invertebrates, the association of claudins with tight junctions has been conclusively documented only in vertebrates. Here we report the sequencing, phylogenetic analysis and comprehensive spatiotemporal expression analysis of the entire claudin gene family in the basal extant vertebrate, the sea lamprey. Our results demonstrate that clear orthologues to about half of all mammalian claudins are present in the lamprey, suggesting that at least one round of whole genome duplication contributed to the diversification of this gene family. Expression analysis revealed that claudins are expressed in discrete and specific domains, many of which represent vertebrate-specific innovations, such as in cranial ectodermal placodes and the neural crest; whereas others represent structures characteristic of chordates, e.g. pronephros, notochord, somites, endostyle and pharyngeal arches. By comparing the embryonic expression of claudins in the lamprey to that of other vertebrates, we found that ancestral expression patterns were often preserved in higher vertebrates. Morpholino mediated loss of Cldn3b demonstrated a functional role for this protein in placode and pharyngeal arch morphogenesis. Taken together, our data provide novel insights into the origins and evolution of the claudin gene family and the significance of claudin proteins in the evolution of vertebrates.", "date": "2016", "date_type": "published", "publication": "International Journal of Developmental Biology", "volume": "60", "number": "1-3", "publisher": "University of the Basque Country Press", "pagerange": "39-51", "id_number": "CaltechAUTHORS:20160601-081405569", "issn": "0214-6282", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160601-081405569", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Research Foundation of South Africa", "grant_number": "80717" } ] }, "doi": "10.1387/ijdb.150364nn", "primary_object": { "basename": "IntJDevBiol-150364nn-SuppMaterial.pdf", "url": "https://authors.library.caltech.edu/records/9mnc6-fda82/files/IntJDevBiol-150364nn-SuppMaterial.pdf" }, "related_objects": [ { "basename": "ft39.pdf", "url": "https://authors.library.caltech.edu/records/9mnc6-fda82/files/ft39.pdf" } ], "resource_type": "article", "pub_year": "2016", "author_list": "Mukendi, Christian; Dean, Nicholas; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w230v-05p08", "eprint_id": 65504, "eprint_status": "archive", "datestamp": "2023-08-22 17:06:08", "lastmod": "2024-01-13 16:44:37", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" } ] }, "title": "The Neural Crest Migrating into the Twenty-First Century", "ispublished": "unpub", "full_text_status": "public", "keywords": "Neural crest; Craniofacial skeleton; Peripheral nervous system; Embryo; Vertebrates", "note": "\u00a9 2016 Elsevier Inc.\n\nAccepted Version - nihms824984.pdf
", "abstract": "From the initial discovery of the neural crest over 150 years ago to the seminal studies of Le Douarin and colleagues in the latter part of the twentieth century, understanding of the neural crest has moved from the descriptive to the experimental. Now, in the twenty-first century, neural crest research has migrated into the genomic age. Here, we reflect upon the major advances in neural crest biology and the open questions that will continue to make research on this incredible vertebrate cell type an important subject in developmental biology for the century to come.", "date": "2016", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "Cambridge, MA", "pagerange": "115-134", "id_number": "CaltechAUTHORS:20160321-080936255", "isbn": "9780128029763", "book_title": "Essays on Developmental Biology", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160321-080936255", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Wassarman-Paul-M", "name": { "family": "Wassarman", "given": "Paul M." }, "orcid": "0000-0003-0924-4039" } ] }, "doi": "10.1016/bs.ctdb.2015.12.003", "pmcid": "PMC5100668", "primary_object": { "basename": "nihms824984.pdf", "url": "https://authors.library.caltech.edu/records/w230v-05p08/files/nihms824984.pdf" }, "resource_type": "book_section", "pub_year": "2016", "author_list": "Bronner, Marianne E. and Sim\u00f5es-Costa, Marcos" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/79ctq-2q759", "eprint_id": 62463, "eprint_status": "archive", "datestamp": "2023-08-20 09:09:54", "lastmod": "2023-10-25 17:10:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uribe-Rosa-A", "name": { "family": "Uribe", "given": "Rosa A." }, "orcid": "0000-0002-0427-4493" }, { "id": "Buzzi-Ail\u00edn-L", "name": { "family": "Buzzi", "given": "Ail\u00edn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Strobl-Mazzulla-Pablo-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" } ] }, "title": "Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 Uribe et al. This article is distributed under the terms of an Attribution\u2013Noncommercial\u2013Share Alike\u2013No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). \n\nSubmitted: 16 March 2015; Accepted: 15 October 2015. \n\nWe thank Dr. M. Barembaum for pCIG-Dlx3 and pCIG-Dlx5 constructs and for insight throughout this study. This work was supported by grants from the Consejo Nacional de Investigaciones Cient\u00edficas (CON ICET-PIP D4574) and Agencia Nacional de Promocion Cientifica y Tecnologica (PICT 2011-0500) to P.H. Strobl-Mazzulla and by the Consejo Nacional de Investigaciones Cient\u00edficas-National Science Foundation grant (CON ICET-NSF D2445) to P.H. Strobl-Mazzulla, as well as by the following supplements from the National Institutes of Health: DC011577 to R.A. Uribe and DC011577 and DE16459 to M.E. Bronner. \n\nThe authors declare no competing financial interests.\n\nPublished - J_Cell_Biol-2015-Uribe-815-27.pdf
Supplemental Material - JCB_201503071_sm.pdf
", "abstract": "In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.", "date": "2015-11-23", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "211", "number": "4", "publisher": "Rockefeller University Press", "pagerange": "815-827", "id_number": "CaltechAUTHORS:20151130-144005066", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151130-144005066", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Consejo Nacional de Investigaciones Cient\u00edficas (CONICET)", "grant_number": "CONICET-PIP D4574" }, { "agency": "Agencia Nacional de Promocion Cientifica y Tecnologica", "grant_number": "PICT 2011-0500" }, { "agency": "Consejo Nacional de Investigaciones Cient\u00edficas (CONICET)", "grant_number": "CONICET-NSF D2445" }, { "agency": "NIH", "grant_number": "DC011577" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1083/jcb.201503071", "pmcid": "PMC4657164", "primary_object": { "basename": "J_Cell_Biol-2015-Uribe-815-27.pdf", "url": "https://authors.library.caltech.edu/records/79ctq-2q759/files/J_Cell_Biol-2015-Uribe-815-27.pdf" }, "related_objects": [ { "basename": "JCB_201503071_sm.pdf", "url": "https://authors.library.caltech.edu/records/79ctq-2q759/files/JCB_201503071_sm.pdf" } ], "resource_type": "article", "pub_year": "2015", "author_list": "Uribe, Rosa A.; Buzzi, Ail\u00edn L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rgj63-kek33", "eprint_id": 61972, "eprint_status": "archive", "datestamp": "2023-08-20 09:07:30", "lastmod": "2023-10-25 16:07:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolution: On the crest of becoming vertebrate", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2015 Macmillan Publishers Limited. \n\nPublished online 28 October 2015.", "abstract": "The discovery of cells in an invertebrate that share several features with vertebrate neural-crest cells provides insights into how this vital vertebrate cell population might have evolved.", "date": "2015-11-19", "date_type": "published", "publication": "Nature", "volume": "527", "number": "7578", "publisher": "Nature Publishing Group", "pagerange": "311-312", "id_number": "CaltechAUTHORS:20151109-083417307", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151109-083417307", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechAUTHORS", "doi": "10.1038/nature15645", "resource_type": "article", "pub_year": "2015", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/07db4-1m717", "eprint_id": 60373, "eprint_status": "archive", "datestamp": "2023-08-20 08:58:56", "lastmod": "2023-10-24 16:29:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uribe-Rosa-A", "name": { "family": "Uribe", "given": "Rosa A." }, "orcid": "0000-0002-0427-4493" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons\u2013Noncommercial\u2013Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0).\n\nSubmitted February 25, 2015. Revised August 6, 2015. Accepted September 2, 2015. Published online before print September 9, 2015. \n\nWe would like to thank Charles Sagerstr\u00f6m for the pCS2\u2010pbcab\u2010myc construct, Tatiana Hochgreb\u2010H\u00e4gele for foxa1 cDNA, Shuo Lin for pdx1 cDNA and Jeff Gross for ptch2 cDNA. We thank the Caltech Beckman Institute Biological Imaging Center and Fish Facility, Marcos Sim\u00f5es\u2010Costa for advice with double fluorescent in situ hybridization and Martha Henderson and David Mayorga for fish care. Research was supported from grants from NIH (DE024157) to M.E.B., from NIH F32 (HD080343) to R.A.U. and a Burroughs Wellcome Fund Postdoctoral Enrichment Program Fellowship to R.A.U.\n\nPublished - 3728.full.pdf
Supplemental Material - CombinedSupMats.pdf
Supplemental Material - mc-E15-02-0112-s01.mov
Supplemental Material - mc-E15-02-0112-s02.mov
", "abstract": "During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here, we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number and the mitotic activity of neural crest cells in the vicinity of the gut, while having no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3 depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung's disease, was misregulated within the gut following loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into and colonization of the gut such that its loss leads to severe defects in enteric nervous system development.", "date": "2015-11-01", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "26", "number": "21", "publisher": "American Society for Cell Biology", "pagerange": "3728-3740", "id_number": "CaltechAUTHORS:20150921-110226858", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150921-110226858", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD080343" }, { "agency": "Burroughs Wellcome Fund" } ] }, "doi": "10.1091/mbc.E15-02-0112", "pmcid": "PMC4626059", "primary_object": { "basename": "mc-E15-02-0112-s01.mov", "url": "https://authors.library.caltech.edu/records/07db4-1m717/files/mc-E15-02-0112-s01.mov" }, "related_objects": [ { "basename": "mc-E15-02-0112-s02.mov", "url": "https://authors.library.caltech.edu/records/07db4-1m717/files/mc-E15-02-0112-s02.mov" }, { "basename": "3728.full.pdf", "url": "https://authors.library.caltech.edu/records/07db4-1m717/files/3728.full.pdf" }, { "basename": "CombinedSupMats.pdf", "url": "https://authors.library.caltech.edu/records/07db4-1m717/files/CombinedSupMats.pdf" } ], "resource_type": "article", "pub_year": "2015", "author_list": "Uribe, Rosa A. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/711c8-n6c27", "eprint_id": 60877, "eprint_status": "archive", "datestamp": "2023-08-20 08:39:11", "lastmod": "2023-10-24 22:05:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" }, "orcid": "0000-0002-0495-7104" }, { "id": "Bajpai-Ruchi", "name": { "family": "Bajpai", "given": "Ruchi" }, "orcid": "0000-0001-7737-3567" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). \n\nReceived: September 8, 2014; Revised: August 27, 2015; Accepted: August 28, 2015; Published: October 1, 2015. \n\nWe thank Drs. Chathurani Jayasena and Crystal Rogers and Erin Moran for technical assistance. This work was funded by DE024157 (to M.E.B.) and the Sigrid Juselius Foundation, Finnish Cultural Foundation, Jane and Aatos Erkko Foundation, V\u00e4re Foundation, and Ella and Georg Ehrnrooth Foundation (to L.K.).\n\nPublished - 1-s2.0-S2213671115002519-main.pdf
Supplemental Material - mmc1.pdf
", "abstract": "Premigratory neural crest cells comprise a transient, embryonic population that arises within the CNS, but subsequently migrates away and differentiates into many derivatives. Previously, premigratory neural crest could not be maintained in a multipotent, adhesive state without spontaneous differentiation. Here, we report conditions that enable maintenance of neuroepithelial \"crestospheres\" that self-renew and retain multipotency for weeks. Moreover, under differentiation conditions, these cells can form multiple derivatives in vitro and in vivo after transplantation into chick embryos. Similarly, human embryonic stem cells directed to a neural crest fate can be maintained as crestospheres and subsequently differentiated into several derivatives. By devising conditions that maintain the premigratory state in vitro, these results demonstrate that neuroepithelial neural crest precursors are capable of long-term self-renewal. This approach will help uncover mechanisms underlying their developmental potential, differentiation and, together with the induced pluripotent stem cell techniques, the pathology of human neurocristopathies.", "date": "2015-10-13", "date_type": "published", "publication": "Stem Cell Reports", "volume": "5", "number": "4", "publisher": "Cell Press", "pagerange": "499-507", "id_number": "CaltechAUTHORS:20151007-132148940", "issn": "2213-6711", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151007-132148940", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "Sigrid Juselius Foundation" }, { "agency": "Finnish Cultural Foundation" }, { "agency": "Jane and Aatos Erkko Foundation" }, { "agency": "V\u00e4re Foundation" }, { "agency": "Ella and Georg Ehrnrooth Foundation" } ] }, "doi": "10.1016/j.stemcr.2015.08.017", "pmcid": "PMC4625028", "primary_object": { "basename": "1-s2.0-S2213671115002519-main.pdf", "url": "https://authors.library.caltech.edu/records/711c8-n6c27/files/1-s2.0-S2213671115002519-main.pdf" }, "related_objects": [ { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/711c8-n6c27/files/mmc1.pdf" } ], "resource_type": "article", "pub_year": "2015", "author_list": "Kerosuo, Laura; Nie, Shuyi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4f0dq-wyf52", "eprint_id": 59442, "eprint_status": "archive", "datestamp": "2023-08-20 08:11:49", "lastmod": "2023-10-23 20:36:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Stone-Michael", "name": { "family": "Stone", "given": "Michael" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Axud1 Integrates Wnt Signaling and Transcriptional Inputs to Drive Neural Crest Formation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 Elsevier. \n\nReceived: February 23, 2015; Revised: May 26, 2015; Accepted: June 29, 2015; Published: August 6, 2015. \n\nWe thank Joanne Tan-Cabugao, Brian Jun, and Daniel S.E. Koo for technical assistance. This work was supported by NIH grants DE024157 and HD037105 to M.E.B. M.S.-C. was funded by a fellowship from the Pew Fellows Program in Biomedical Sciences and by NIH grant K99DE024232. M.S. was funded by a Caltech SURF fellowship. \n\nAUTHOR CONTRIBUTIONS: M.S.-C. and M.E.B. conceived and designed the experimental approach. M.S.-C. and M.S. performed the experiments and analyzed the data. M.S.-C. and M.E.B. wrote the manuscript.\n\nAccepted Version - nihms-709420.pdf
Supplemental Material - mmc1.pdf
", "abstract": "Neural crest cells are induced at the neural plate border by the combined action of transcription factors and signaling molecules. Here, we show that Axud1, a downstream effector of Wnt signaling, represents a critical missing link that integrates signaling and transcriptional cues to mediate neural crest formation. Axud1 is a transcription factor expressed in neural crest progenitors in a Wnt1/\u03b2-catenin-dependent manner. Axud1 loss leads to downregulation of multiple genes involved in neural crest specification, similar to the effects of Wnt1 knockdown. Importantly, Axud1 is sufficient to rescue neural crest formation after disruption of Wnt signaling. Furthermore, it physically interacts with neural plate border genes Pax7 and Msx1 in vivo to directly activate transcription of stem cell factor FoxD3, initiating the neural crest program. Thus, Axud1 integrates Wnt signaling with transcriptional inputs to endow the neural crest with its unique molecular signature.", "date": "2015-09-14", "date_type": "published", "publication": "Developmental Cell", "volume": "34", "number": "5", "publisher": "Cell Press", "pagerange": "544-554", "id_number": "CaltechAUTHORS:20150812-093923480", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150812-093923480", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Pew Fellows Program in the Biomedical Sciences" }, { "agency": "NIH", "grant_number": "K99DE024232" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" } ] }, "doi": "10.1016/j.devcel.2015.06.024", "pmcid": "PMC4573882", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/4f0dq-wyf52/files/mmc1.pdf" }, "related_objects": [ { "basename": "nihms-709420.pdf", "url": "https://authors.library.caltech.edu/records/4f0dq-wyf52/files/nihms-709420.pdf" } ], "resource_type": "article", "pub_year": "2015", "author_list": "Sim\u00f5es-Costa, Marcos; Stone, Michael; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/r7x6s-j8q91", "eprint_id": 66022, "eprint_status": "archive", "datestamp": "2023-08-20 07:41:44", "lastmod": "2023-10-18 17:03:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Huang-Miller", "name": { "family": "Huang", "given": "Miller" } }, { "id": "McHenry-L-K", "name": { "family": "McHenry", "given": "Lauren K." } }, { "id": "Miller-M-L", "name": { "family": "Miller", "given": "Matthew L." } }, { "id": "Kim-Grace-E", "name": { "family": "Kim", "given": "Grace E." } }, { "id": "Moriarity-B-S", "name": { "family": "Moriarity", "given": "Branden S." } }, { "id": "Miyaoka-Yuichiro", "name": { "family": "Miyaoka", "given": "Yuichiro" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Largaespada-D-A", "name": { "family": "Largaespada", "given": "David A." } }, { "id": "Conklin-B-R", "name": { "family": "Conklin", "given": "Bruce R." } }, { "id": "Ji-Hanlee-P", "name": { "family": "Ji", "given": "Hanlee P." } }, { "id": "Maris-J-M", "name": { "family": "Maris", "given": "John M." } }, { "id": "Matthay-K-K", "name": { "family": "Matthay", "given": "Katherine K." } }, { "id": "Weiss-W-A", "name": { "family": "Weiss", "given": "William A." } } ] }, "title": "Human stem cell-based model of MYCN-driven neuroblastoma", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 American Association for Cancer Research.", "abstract": "Neuroblastoma (NB), a disease of neural crest (NC) origin, is the most common extracranial solid tumor in childhood. High-risk NB patients represent the subgroup with the worst prognosis and frequently harbor amplification of MYCN. Due to its biochemical structure as a transcription factor, MYCN has been difficult to target directly with small molecules. Alternatively, identification of cooperating partners of MYCN-induced tumorigenesis can reveal a more therapeutically viable target, such as anaplastic lymphoma kinase (ALK). While genetically engineered mouse models (GEMMs) of NB driven by MYCN and ALK exist, recent studies have found significant differences between mouse models of disease and the human tumors they are intended to represent. In support of this, mouse and human NC cells differ in development and marker expression, and the genetic requirement for transformation of human cells has been shown to be more complex than mouse cells, suggesting that a human cell-based NB model would be more relevant. To develop a human cell-based model of NB, we started with a normal human induced pluripotent stem (iPS) cell line derived integration-free from a healthy adult and transduced empty vector, ALK F1174L (active mutant), doxycycline-inducible MYCN (DOX-MYCN), or ALK F1174L/DOX-MYCN. These iPS cells were differentiated towards NC cells and subsequently implanted orthotopically into renal capsules of mice fed on dox chow. Within 3 months, 60% of mice developed tumors with ALK F1174L/DOX-MYCN, 10% with DOX-MYCN, and 0% with both empty vector and ALK F1174L alone. Tumors were transplantable and demonstrated histologic characteristics consistent with NB, including morphology and expression markers. Thus, we are demonstrating the first, to our knowledge, human stem cell-based model of NB. We are using this system to further investigate similarities and differences with GEMMs of NB, test and establish novel candidate drivers of NB, and evaluate potential therapeutic options.", "date": "2015-08-01", "date_type": "published", "publication": "Cancer Research", "volume": "75", "number": "S15", "publisher": "American Association for Cancer Research", "pagerange": "Art. No. 3230", "id_number": "CaltechAUTHORS:20160408-110803385", "issn": "0008-5472", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160408-110803385", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1158/1538-7445.AM2015-3230", "resource_type": "article", "pub_year": "2015", "author_list": "Huang, Miller; McHenry, Lauren K.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4nmmh-r6088", "eprint_id": 57149, "eprint_status": "archive", "datestamp": "2023-08-20 06:10:14", "lastmod": "2023-10-23 17:06:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barriga-Elias-H", "name": { "family": "Barriga", "given": "Elias H." } }, { "id": "Trainor-Paul-A", "name": { "family": "Trainor", "given": "Paul A." }, "orcid": "0000-0003-2774-3624" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Mayor-Roberto", "name": { "family": "Mayor", "given": "Roberto" } } ] }, "title": "Animal models for studying neural crest development: is the mouse different?", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 Published by The Company of Biologists Ltd. \n\nReceived January 7, 2015. Accepted March 11, 2015. \n\nR.M. is supported by grants from the Medical Research Council [MR/J000655/1] and Wellcome Trust. E.H.B. is supported by a Quantissue Fellowship [4505]. M.B. is supported by National Institutes of Health grants [DE024157 and HD037105]. P.A.T. is supported by the Stowers Institute for Medical Research and the National Institute of Dental and Craniofacial Research [DE 016082].\n\nPublished - Development-2015-Barriga-1555-60.pdf
", "abstract": "The neural crest is a uniquely vertebrate cell type and has been well studied in a number of model systems. Zebrafish, Xenopus and chick embryos largely show consistent requirements for specific genes in early steps of neural crest development. By contrast, knockouts of homologous genes in the mouse often do not exhibit comparable early neural crest phenotypes. In this Spotlight article, we discuss these species-specific differences, suggest possible explanations for the divergent phenotypes in mouse and urge the community to consider these issues and the need for further research in complementary systems.", "date": "2015-05-01", "date_type": "published", "publication": "Development", "volume": "142", "number": "9", "publisher": "Company of Biologists", "pagerange": "1555-1560", "id_number": "CaltechAUTHORS:20150501-111151491", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150501-111151491", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Medical Research Council", "grant_number": "MR/J000655/1" }, { "agency": "Wellcome Trust" }, { "agency": "Quantissue Fellowship", "grant_number": "4505" }, { "agency": "NIH", "grant_number": "DE024157" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Stowers Institute for Medical Research" }, { "agency": "NIH", "grant_number": "DE 016082" }, { "agency": "National Institute of Dental and Craniofacial Research (NIDCR)" } ] }, "doi": "10.1242/dev.121590", "pmcid": "PMC6514397", "primary_object": { "basename": "Development-2015-Barriga-1555-60.pdf", "url": "https://authors.library.caltech.edu/records/4nmmh-r6088/files/Development-2015-Barriga-1555-60.pdf" }, "resource_type": "article", "pub_year": "2015", "author_list": "Barriga, Elias H.; Trainor, Paul A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/55y87-zw717", "eprint_id": 57553, "eprint_status": "archive", "datestamp": "2023-08-20 05:52:58", "lastmod": "2023-10-23 17:31:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Green-Stephen-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolution of vertebrates as viewed from the crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2015 Macmillan Publishers Limited.\n\nReceived 10 September 2014; accepted 5 February 2015.\n\nWe would like to thank H. Parker, C. Rogers and L. Kerosuo for their comments and helpful discussion on this manuscript. This work was supported by National Institutes of Health (NIH) grant R01NS086907. M.S.-C. was funded by a fellowship from the Pew Foundation and by NIH grant 1K99DE024232.\n\nAccepted Version - nihms-825457.pdf
", "abstract": "The origin of vertebrates was accompanied by the advent of a novel cell type: the neural crest. Emerging from the central nervous system, these cells migrate to diverse locations and differentiate into numerous derivatives. By coupling morphological and gene regulatory information from vertebrates and other chordates, we describe how addition of the neural-crest-specification program may have enabled cells at the neural plate border to acquire multipotency and migratory ability. Analysis of the topology of the neural crest gene regulatory network can serve as a useful template for understanding vertebrate evolution, including elaboration of neural crest derivatives.", "date": "2015-04-23", "date_type": "published", "publication": "Nature", "volume": "520", "number": "7548", "publisher": "Nature Publishing Group", "pagerange": "474-482", "id_number": "CaltechAUTHORS:20150515-073859339", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150515-073859339", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01NS086907" }, { "agency": "Pew Foundation" }, { "agency": "NIH", "grant_number": "1K99DE024232" } ] }, "doi": "10.1038/nature14436", "pmcid": "PMC5100666", "primary_object": { "basename": "nihms-825457.pdf", "url": "https://authors.library.caltech.edu/records/55y87-zw717/files/nihms-825457.pdf" }, "resource_type": "article", "pub_year": "2015", "author_list": "Green, Stephen A.; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/kdw3e-jmr56", "eprint_id": 56752, "eprint_status": "archive", "datestamp": "2023-08-22 15:19:11", "lastmod": "2023-10-23 15:47:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dual developmental role of transcriptional regulator Ets1 in Xenopus cardiac neural crest vs. heart mesoderm", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Ets1; Cardiac neural crest; Heart mesoderm; Aortic arch artery; Endocardium", "note": "\u00a9 2015 The Author. Published on behalf of the European Society of Cardiology. \n\nReceived 24 November 2014; revised 2 February 2015; accepted 5 February 2015; online publish-ahead-of-print 17 February 2015. \n\nThis work was supported by American Heart Association postdoctoral fellowship (12POST8610001) and National Institutes of Health career grant (1K99DE022796) to S.N., and National Institutes of Health (R01HD037105) to M.E.B.", "abstract": "Aims: Ets1 is an important transcription factor that is expressed in both the cardiac neural crest (NC) and heart mesoderm of vertebrate embryos. Moreover, Ets1 deletion in humans results in congenital heart abnormalities. To clarify the functional contributions of Ets1 in cardiac NC vs. heart mesoderm, we performed tissue-targeted loss-of-function analysis to compare the relative roles of Ets1 in these two tissues during heart formation using Xenopus embryos as a model system.\n\nMethods and results: We confirmed by in situ hybridization analysis that Ets1 is expressed in NC and heart mesoderm during embryogenesis. Using a translation-blocking antisense morpholino to knockdown Ets1 protein selectively in the NC, we observed defects in NC delamination from the neural tube, collective cell migration, as well as segregation of NC streams in the cranial and cardiac regions. Many cardiac NC cells failed to reach their destination in the heart, resulting in defective aortic arch artery formation. A different set of defects was noted when Ets1 knockdown was targeted to heart mesoderm. The formation of the primitive heart tube was dramatically delayed and the endocardial tissue appeared depleted. As a result, the conformation of the heart was severely disrupted. In addition, the outflow tract septum was missing, and trabeculae formation in the ventricle was abolished.\n\nConclusion: Our study shows that Ets1 is required in both the cardiac NC and heart mesoderm, albeit for different aspects of heart formation. Our results reinforce the suggestion that proper interaction between these tissues is critical for normal heart development.", "date": "2015-04-01", "date_type": "published", "publication": "Cardiovascular Research", "volume": "106", "number": "1", "publisher": "Oxford University Press", "pagerange": "67-75", "id_number": "CaltechAUTHORS:20150420-080027513", "issn": "0008-6363", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150420-080027513", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "12POST8610001" }, { "agency": "NIH", "grant_number": "1K99DE022796" }, { "agency": "NIH", "grant_number": "R01HD037105" } ] }, "doi": "10.1093/cvr/cvv043", "pmcid": "PMC4447785", "resource_type": "article", "pub_year": "2015", "author_list": "Nie, Shuyi and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5wg6v-25e31", "eprint_id": 54314, "eprint_status": "archive", "datestamp": "2023-08-22 15:18:32", "lastmod": "2023-10-20 15:48:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hochgreb-H\u00e4gele-T", "name": { "family": "Hochgreb-H\u00e4gele", "given": "Tatiana" } }, { "id": "Koo-Daniel-E-S", "name": { "family": "Koo", "given": "Daniel E. S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation", "ispublished": "pub", "full_text_status": "public", "keywords": "Cartilage maturation; Ossification; p53; Neural crest; Zebrafish", "note": "\u00a9 2015 Elsevier Inc. \n\nReceived 10 January 2015, Accepted 12 January 2015, Available online 27 January 2015. \n\nWe thank Dr. Tatjana Sauka-Spengler for valuable discussions, Dr. Sujata Bhattacharyya for comments on the manuscript, and Dr. Le Trinh and Dr. Scott Fraser for helpful advice. We thank Leigh Ann Fletcher, Kwok Su and David Mayorga for fish care; Neha Das Messenger, Ilana Solomon and Joyce De Leon for technical support; and Dr. Robert Kelsh for sharing the Tg(-4.9sox10:EGFP) line.\n\nFinancial support: T.H.H. was supported by a Pew Latin American Fellowship in the Biomedical Sciences and by a California Institute for Regenerative Medicine Training Grant (T2-00006). This work was supported by NIH grants HD037105 and HG004071 (M.E.B).\n\nAuthor contributions: T.H.H. designed and performed experiments, analyzed data, and wrote the manuscript.\n\nD.E.S.K. performed experiments, analyzed data, and edited the manuscript.\n\nM.E.B. supervised the work including helping with experimental design, data, analysis, and manuscript preparation. \n\nEthics statement for animal experimentation This study was performed in strict accordance with the guidelines in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved Institutional Animal Care and Use Committee (IACUC) protocol at the California Institute of Technology.\n\nSupplemental Material - mmc1.pdf
", "abstract": "Jaw formation involves an intricate series of molecular events, whereby a chondrogenic scaffold precedes osteogenesis. The mechanisms coupling timing of cartilage maturation to onset of bone differentiation are poorly understood, particularly for neural crest-derived bones of the head. Here we present a novel zebrafish gene/protein-trap Citrine-fusion line that reveals transient expression of the zinc-finger protein Znf385C in maturing chondrocytes of the jaw. Functional analysis shows that loss of Znf385C disrupts a distinct peak of p21^(cip1/waf1) expression in the chondrocytes, as well as causes premature ossification of the zebrafish jaw. We find that Znf385C is expressed as two splice variants which act differentially to activate p21^(cip1/waf1) and/or interact with p53 in subcellular compartments. Taken together, the results suggest that Znf385C acts as a developmental switch for p53 function that modulates cell cycle arrest of chondrocytes and regulates timing of jaw cartilage maturation and ossification.", "date": "2015-04-01", "date_type": "published", "publication": "Developmental Biology", "volume": "400", "number": "1", "publisher": "Elsevier", "pagerange": "23-32", "id_number": "CaltechAUTHORS:20150203-081805904", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150203-081805904", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pew Latin American Fellowship in Biomedical Sciences" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "T2-00006" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "HG004071" } ] }, "doi": "10.1016/j.ydbio.2015.01.011", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/5wg6v-25e31/files/mmc1.pdf" }, "resource_type": "article", "pub_year": "2015", "author_list": "Hochgreb-H\u00e4gele, Tatiana; Koo, Daniel E. S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jy3e4-jfm78", "eprint_id": 55712, "eprint_status": "archive", "datestamp": "2023-08-20 05:19:41", "lastmod": "2023-10-20 23:03:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Confetti Clarifies Controversy: Neural Crest Stem Cells Are Multipotent", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2015 Elsevier Inc.", "abstract": "Neural crest precursors generate diverse cell lineages during development, which have been proposed to\narise either from multipotent precursor cells or pools of heterogeneous, restricted progenitors. Now in Cell\nStem Cell, Baggiolini et al. (2015) perform rigorous in vivo lineage tracing to show that individual neural crest\nprecursors are multipotent.", "date": "2015-03-05", "date_type": "published", "publication": "Cell Stem Cell", "volume": "16", "number": "3", "publisher": "Elsevier", "pagerange": "217-218", "id_number": "CaltechAUTHORS:20150311-142256996", "issn": "1934-5909", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150311-142256996", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.stem.2015.02.016", "resource_type": "article", "pub_year": "2015", "author_list": "Bronner, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ja1pz-rsj76", "eprint_id": 55141, "eprint_status": "archive", "datestamp": "2023-08-20 05:17:44", "lastmod": "2023-10-20 21:51:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Letter from the editor \u2013 issue 399/1\u20131 March 2015", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2015 Elsevier Inc.", "abstract": "The onset of 2015 provides a unique opportunity to reflect on the events of the past year in the life of our journal, Developmental Biology. In 2014, we increased the numbers of Review Articles, and initiated several new types of papers, including Short Communications, Technical Reports, and Resources Articles. We also published several Special Issues on topics of interest to the community, and we have numerous others that are currently in the works.", "date": "2015-03-01", "date_type": "published", "publication": "Developmental Biology", "volume": "399", "number": "1", "publisher": "Elsevier", "pagerange": "1", "id_number": "CaltechAUTHORS:20150224-084240827", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150224-084240827", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2015.01.024", "resource_type": "article", "pub_year": "2015", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xny63-m7h97", "eprint_id": 51542, "eprint_status": "archive", "datestamp": "2023-08-22 14:57:51", "lastmod": "2023-10-18 16:50:08", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Butler-S-J", "name": { "family": "Butler", "given": "Samantha J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "From classical to current: Analyzing peripheral nervous system and spinal cord lineage and fate", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Spinal cord; Peripheral nervous system; Lineage neuronal subtype", "note": "\u00a9 2014 Published by Elsevier Inc. \n\nReceived 18 June 2014, Revised 22 September 2014, Accepted 25 September 2014, Available online 24 October 2014. \n\nThe authors declare they have no conflict of interest. We thank Donna Crandall for her invaluable help preparing the figures, Katrina Adams, Bennett Novitch, Ankur Saxena and Supraja Varadarajan for images, Jane Johnson for discussions and Bennett Novitch for comments on the manuscript. Samantha Butler is supported by grants from NIH/NINDS (NS-063999, NS085097), CIRM (RB5-07320) and the Craig H. Neilsen Foundation (no. 284402). Marianne Bronner is support by NIH grants HD037105, DE16459, and DE02415.\n\nAccepted Version - nihms652521.pdf
", "abstract": "During vertebrate development, the central (CNS) and peripheral nervous systems (PNS) arise from the neural plate. Cells at the margin of the neural plate give rise to neural crest cells, which migrate extensively throughout the embryo, contributing to the majority of neurons and all of the glia of the PNS. The rest of the neural plate invaginates to form the neural tube, which expands to form the brain and spinal cord. The emergence of molecular cloning techniques and identification of fluorophores like Green Fluorescent Protein (GFP), together with transgenic and electroporation technologies, have made it possible to easily visualize the cellular and molecular events in play during nervous system formation. These lineage-tracing techniques have precisely demonstrated the migratory pathways followed by neural crest cells and increased knowledge about their differentiation into PNS derivatives. Similarly, in the spinal cord, lineage-tracing techniques have led to a greater understanding of the regional organization of multiple classes of neural progenitor and post-mitotic neurons along the different axes of the spinal cord and how these distinct classes of neurons assemble into the specific neural circuits required to realize their various functions. Here, we review how both classical and modern lineage and marker analyses have expanded our knowledge of early peripheral nervous system and spinal cord development.", "date": "2015-02-15", "date_type": "published", "publication": "Developmental Biology", "volume": "398", "number": "2", "publisher": "Elsevier", "pagerange": "135-146", "id_number": "CaltechAUTHORS:20141111-070043986", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141111-070043986", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Institute of Neurological Disorders and Stroke (NINDS)", "grant_number": "NS-063999" }, { "agency": "National Institute of Neurological Disorders and Stroke (NINDS)", "grant_number": "NS085097" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "RB5-07320" }, { "agency": "Craig H. Neilsen Foundation", "grant_number": "284402" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "NIH", "grant_number": "DE02415" } ] }, "doi": "10.1016/j.ydbio.2014.09.033", "pmcid": "PMC4845735", "primary_object": { "basename": "nihms652521.pdf", "url": "https://authors.library.caltech.edu/records/xny63-m7h97/files/nihms652521.pdf" }, "resource_type": "article", "pub_year": "2015", "author_list": "Butler, Samantha J. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vxfsy-c6p90", "eprint_id": 53330, "eprint_status": "archive", "datestamp": "2023-08-20 04:35:55", "lastmod": "2023-10-19 14:46:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Establishing neural crest identity: a gene regulatory recipe", "ispublished": "pub", "full_text_status": "public", "keywords": "Gene regulation, Migration, Neural crest, Neural plate border, Signaling, Transcription factors", "note": "\u00a9 2015 Published by The Company of Biologists Ltd.\n\nFunding:\nWork in the laboratory of M.E.B. is supported by the National Institutes of Health.\nM.S.-C. was funded by the Pew fellows Program in the Biomedical Sciences and\nby a grant from the National Institute of Dental and Craniofacial Research.\nDeposited in PMC for release after 12 months.\n\nCompeting interests:\nThe authors declare no competing financial interests.\n\nPublished - Development-2015-Sim\u00f5es-Costa-242-57.pdf
", "abstract": "The neural crest is a stem/progenitor cell population that contributes\nto a wide variety of derivatives, including sensory and autonomic\nganglia, cartilage and bone of the face and pigment cells of the skin.\nUnique to vertebrate embryos, it has served as an excellent model\nsystem for the study of cell behavior and identity owing to its\nmultipotency, motility and ability to form a broad array of cell types.\nNeural crest development is thought to be controlled by a suite of\ntranscriptional and epigenetic inputs arranged hierarchically in a gene\nregulatory network. Here, we examine neural crest development from\na gene regulatory perspective and discuss how the underlying\ngenetic circuitry results in the features that define this unique cell\npopulation.", "date": "2015-01-15", "date_type": "published", "publication": "Development", "volume": "142", "number": "2", "publisher": "Company of Biologists", "pagerange": "242-257", "id_number": "CaltechAUTHORS:20150108-091905950", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150108-091905950", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "Pew Fellows Program in the Biomedical Sciences" }, { "agency": "National Institute of Dental and Craniofacial Research (NIDCR)" } ] }, "doi": "10.1242/dev.105445", "pmcid": "PMC4302844", "primary_object": { "basename": "Development-2015-Sim\u00f5es-Costa-242-57.pdf", "url": "https://authors.library.caltech.edu/records/vxfsy-c6p90/files/Development-2015-Sim\u00f5es-Costa-242-57.pdf" }, "resource_type": "article", "pub_year": "2015", "author_list": "Sim\u00f5es-Costa, Marcos and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sp3w5-sgh70", "eprint_id": 50408, "eprint_status": "archive", "datestamp": "2023-08-22 14:44:17", "lastmod": "2023-10-17 23:26:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uy-B-R", "name": { "family": "Uy", "given": "Benjamin R." }, "orcid": "0000-0003-0438-880X" }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Koo-Daniel-E-S", "name": { "family": "Koo", "given": "Daniel E. S." } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolutionarily conserved role for SoxC genes in neural crest specification and neuronal differentiation", "ispublished": "pub", "full_text_status": "public", "keywords": "SoxC; Neural crest; Neuronal differentiation; Lamprey; Evolution", "note": "\u00a9 2014 Elsevier Inc. \n\nReceived 29 September 2013, Revised 6 August 2014, Accepted 19 September 2014, Available online 5 October 2014. \n\nWe thank Natalya Nikitina for the lamprey neurogenin probe, and Shuyi Nie for sharing her frog expertise, NIH Caltech Training Grant, the Caltech SURF for generous support of BRU. MSC was supported by the Pew Fellows Program in the Biomedical Sciences and a Caltech Cell Center fellowship from the Moore Foundation. This work was supported by NIH Grants DE017911 and NS086907 to MEB.\n\nAccepted Version - nihms-633361.pdf
Supplemental Material - mmc1.pdf
", "abstract": "Members of the Sox family of transcription factors play a variety of critical developmental roles in both vertebrates and invertebrates. Whereas SoxBs and SoxEs are involved in neural and neural crest development, respectively, far less is known about members of the SoxC subfamily. To address this from an evolutionary perspective, we compare expression and function of SoxC genes in neural crest cells and their derivatives in lamprey (Petromyzon marinus), a basal vertebrate, to frog (Xenopus laevis). Analysis of transcript distribution from reveals conservation of lamprey and X. laevis SoxC expression in premigratory neural crest, branchial arches, and cranial ganglia. Moreover, morpholino-mediated loss-of-function of selected SoxC family members demonstrates essential roles in aspects of neural crest development in both organisms. The results suggest important and conserved functions of SoxC genes during vertebrate evolution and a particularly critical, previously unrecognized role in early neural crest specification.", "date": "2015-01-15", "date_type": "published", "publication": "Developmental Biology", "volume": "397", "number": "2", "publisher": "Elsevier", "pagerange": "282-292", "id_number": "CaltechAUTHORS:20141015-112340436", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141015-112340436", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH Caltech Training Grant" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" }, { "agency": "Pew Charitable Trust" }, { "agency": "Gordon and Betty Moore Foundation" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "NS086907" } ] }, "doi": "10.1016/j.ydbio.2014.09.022", "pmcid": "PMC4545591", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/sp3w5-sgh70/files/mmc1.pdf" }, "related_objects": [ { "basename": "nihms-633361.pdf", "url": "https://authors.library.caltech.edu/records/sp3w5-sgh70/files/nihms-633361.pdf" } ], "resource_type": "article", "pub_year": "2015", "author_list": "Uy, Benjamin R.; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/js448-n9k98", "eprint_id": 51757, "eprint_status": "archive", "datestamp": "2023-08-22 14:30:08", "lastmod": "2023-10-18 17:07:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hu-Na", "name": { "family": "Hu", "given": "Na" } }, { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "S\u00e1nchez-V\u00e1squez-E", "name": { "family": "S\u00e1nchez-V\u00e1squez", "given": "Estefania" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; epigenetic; DNMT3B; Sox10; DNA methylation", "note": "\u00a9 2014 National Academy of Sciences. \n\nPublished online before print December 1, 2014, doi:10.1073/pnas.1318408111 PNAS December 1, 2014\n\nEdited by Joseph R. Ecker, Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA, and approved November 5, 2014 (received for review September 30, 2013) \n\nWe thank Drs. T. Sauka-Spengler and M. Barembaum for helpful discussions. This work was supported by Grants F31DE021643 and Q:10 5 T32 GM07616 (to N.H.) and HD037105 and DE16459 (to M.E.B.). \n\nAuthor contributions: N.H., P.H.S.-M., M.S.-C., and M.E.B. designed research; N.H., P.H.S.-M., M.S.-C., and E.S.-V. performed research; N.H., P.H.S.-M., M.S.-C., E.S.-V., and M.E.B. analyzed data; and N.H., P.H.S.-M., and M.E.B. wrote the paper. \n\nThe authors declare no conflict of interest. \n\nThis article is a PNAS Direct Submission. \n\nN.H. and P.H.S.-M. contributed equally to this work. \n\nThis article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1318408111/-/DCSupplemental.\n\nPublished - 17911.full.pdf
Supplemental Material - pnas.201318408SI.pdf
", "abstract": "Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neural tube cells to generate emigrating neural crest cells. DNMT3B knockdown results in up-regulation of neural crest markers, prolonged neural crest emigration, and subsequent precocious neuronal differentiation of the trigeminal ganglion. We find that DNMT3B binds to the promoter of Sox10, known to be important for neural crest emigration and lineage acquisition. Bisulfite sequencing further reveals methylation of the Sox10 promoter region upon cessation of emigration in normal embryos, whereas this mark is reduced after DNMT3B loss. Taken together, these results reveal the importance of DNA methylation in regulating the ability of neural tube cells to produce neural crest cells and the timing of peripheral neuron differentiation.", "date": "2014-12-16", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "111", "number": "50", "publisher": "National Academy of Sciences", "pagerange": "17911-17916", "id_number": "CaltechAUTHORS:20141114-094819602", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141114-094819602", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "F31DE021643" }, { "agency": "NIH", "grant_number": "5 T32 GM07616" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1073/pnas.1318408111", "pmcid": "PMC4273375", "primary_object": { "basename": "17911.full.pdf", "url": "https://authors.library.caltech.edu/records/js448-n9k98/files/17911.full.pdf" }, "related_objects": [ { "basename": "pnas.201318408SI.pdf", "url": "https://authors.library.caltech.edu/records/js448-n9k98/files/pnas.201318408SI.pdf" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Hu, Na; Strobl-Mazzulla, Pablo H.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sx02a-k2718", "eprint_id": 51478, "eprint_status": "archive", "datestamp": "2023-08-22 14:29:31", "lastmod": "2023-10-18 16:45:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hu-Na", "name": { "family": "Hu", "given": "Na" } }, { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Epigenetic regulation in neural crest development", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Epigenetic; Development", "note": "\u00a9 2014 Elsevier Inc. \n\nReceived 20 March 2014; Received in revised form 17 September 2014; Accepted 25 September 2014; Available online 24 October 2014.\n\nAccepted Version - nihms638464.pdf
", "abstract": "The neural crest is a migratory and multipotent cell population that plays a crucial role in many aspects of embryonic development. In all vertebrate embryos, these cells emerge from the dorsal neural tube then migrate long distances to different regions of the body, where they contribute to formation of many cell types and structures. These include much of the peripheral nervous system, craniofacial skeleton, smooth muscle, and pigmentation of the skin. The best-studied regulatory events guiding neural crest development are mediated by transcription factors and signaling molecules. In recent years, however, growing evidence supports an important role for epigenetic regulation as an additional mechanism for controlling the timing and level of gene expression at different stages of neural crest development. Here, we summarize the process of neural crest formation, with focus on the role of epigenetic regulation in neural crest specification, migration, and differentiation as well as in neural crest related birth defects and diseases.", "date": "2014-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "396", "number": "2", "publisher": "Elsevier", "pagerange": "159-168", "id_number": "CaltechAUTHORS:20141110-080255491", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141110-080255491", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2014.09.034", "pmcid": "PMC4261016", "primary_object": { "basename": "nihms638464.pdf", "url": "https://authors.library.caltech.edu/records/sx02a-k2718/files/nihms638464.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Hu, Na; Strobl-Mazzulla, Pablo H.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vrcty-7wv08", "eprint_id": 49513, "eprint_status": "archive", "datestamp": "2023-08-20 03:25:19", "lastmod": "2023-10-17 21:30:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krumlauf-R", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" } ] }, "title": "A Hox regulatory network of hindbrain segmentation is conserved to the base of vertebrates", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Macmillan Publishers Limited. \n\nReceived 15 May; accepted 31 July 2014. Published online 14 September 2014. \n\nWe thank S. Green for sharing expertise, methods and managing\nthe lamprey facility; T. Sauka-Spengler for in situ hybridization advice; M. Simoes-Costa\nand B. Uy for help with reporter constructs; J. McEllin, C. Nolte, C. Scott and\nL. Wiedemann for discussions and assistance with lamprey hox genes; M. Distel and\nR. Koster for the r3/r5-mCherry zebrafish line; M. Miller for graphic design; A. Ikmi for\nmanuscript comments and the Stowers Institute aquatics facility for zebrafish care.\nH.J.P. and R.K. were supported by the Stowers Institute (RK grant #2013-1001). M.E.B.\nwas supported by grants R01NS086907 and R01DE017911.\n\nAuthor Contributions H.J.P., R.K. and M.E.B. conceived this research program. H.J.P.\nconducted the experiments. H.J.P., R.K. and M.E.B. jointly analysed the data, discussed\nthe ideas and interpretations and wrote the manuscript.\nAuthor Information The sequences for the lamprey hox1wand kreisler transcripts have\nbeen deposited in GenBank under accession numbers KM087087 (hox1w) and\nKM087088 (kreisler). All original source data have been deposited in the Stowers\nInstitute Original Data Repository and are available online at http://odr.stowers.org/\nwebsimr/. Reprints and permissions information is available at www.nature.com/\nreprints. \n\nThe authors declare no competing financial interests. Readers are welcome to\ncomment on the online version of the paper. Correspondence and requests for\nmaterials should be addressed to R.K. (rek@Stowers.org).\n\nAccepted Version - nihms618167.pdf
", "abstract": "A defining feature governing head patterning of jawed vertebrates is a highly conserved gene regulatory network that integrates hindbrain segmentation with segmentally restricted domains of Hox gene expression. Although non-vertebrate chordates display nested domains of axial Hox expression, they lack hindbrain segmentation. The sea lamprey, a jawless fish, can provide unique insights into vertebrate origins owing to its phylogenetic position at the base of the vertebrate tree. It has been suggested that lamprey may represent an intermediate state where nested Hox expression has not been coupled to the process of hindbrain segmentation. However, little is known about the regulatory network underlying Hox expression in lamprey or its relationship to hindbrain segmentation. Here, using a novel tool that allows cross-species comparisons of regulatory elements between jawed and jawless vertebrates, we report deep conservation of both upstream regulators and segmental activity of enhancer elements across these distant species. Regulatory regions from diverse gnathostomes drive segmental reporter expression in the lamprey hindbrain and require the same transcriptional inputs (for example, Kreisler (also known as Mafba), Krox20 (also known as Egr2a)) in both lamprey and zebrafish. We find that lamprey hox genes display dynamic segmentally restricted domains of expression; we also isolated a conserved exonic hox2 enhancer from lamprey that drives segmental expression in rhombomeres 2 and 4. Our results show that coupling of Hox gene expression to segmentation of the hindbrain is an ancient trait with origin at the base of vertebrates that probably led to the formation of rhombomeric compartments with an underlying Hox code.", "date": "2014-10-23", "date_type": "published", "publication": "Nature", "volume": "514", "number": "7523", "publisher": "Nature Publishing Group", "pagerange": "490-493", "id_number": "CaltechAUTHORS:20140909-171653236", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140909-171653236", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Stowers Institute", "grant_number": "RK 2013-1001" }, { "agency": "NIH", "grant_number": "R01NS086907" }, { "agency": "NIH", "grant_number": "R01DE017911" } ] }, "doi": "10.1038/nature13723", "pmcid": "PMC4209185", "primary_object": { "basename": "nihms618167.pdf", "url": "https://authors.library.caltech.edu/records/vrcty-7wv08/files/nihms618167.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Parker, Hugo J.; Bronner, Marianne E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/a8h6s-67k55", "eprint_id": 48835, "eprint_status": "archive", "datestamp": "2023-08-22 13:45:14", "lastmod": "2023-10-17 20:28:13", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches", "ispublished": "pub", "full_text_status": "public", "keywords": "HoxB cluster; Hoxb4a; zebrafish; fluorescent fusion protein", "note": "\u00a9 2014 Wiley Periodicals, Inc. \n\nReceived 5 July 2014; Revised 11 August 2014; Accepted 13 August 2014. Article first published online: 23 AUG 2014. \n\nWe thank David Mayorga for zebrafish husbandry assistance; Ilana Solomon for technical support; Dr. Hugo Parker for scientific feedback; Dr. Bruce Appel for the Sox10:mRFP line; Dr. Uwe Strahle for the Ngn1:nRFP line.\n\nAccepted Version - nihms-623261.pdf
", "abstract": "We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3\u2032 RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.", "date": "2014-10", "date_type": "published", "publication": "Genesis", "volume": "52", "number": "10", "publisher": "Wiley", "pagerange": "858-863", "id_number": "CaltechAUTHORS:20140825-090002879", "issn": "1526-954X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140825-090002879", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P50HG004071" }, { "agency": "Gordon Ross Postdoctoral Fellowship" }, { "agency": "NIH", "grant_number": "5T32NS007251" } ] }, "doi": "10.1002/dvg.22806", "pmcid": "PMC4211940", "primary_object": { "basename": "nihms-623261.pdf", "url": "https://authors.library.caltech.edu/records/a8h6s-67k55/files/nihms-623261.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Saxena, Ankur and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/kceyz-hw842", "eprint_id": 50084, "eprint_status": "archive", "datestamp": "2023-08-22 13:30:43", "lastmod": "2023-10-17 22:29:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ezin-M", "name": { "family": "Ezin", "given": "Maxellende" } }, { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Stage-dependent plasticity of the anterior neural folds to form neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Olfactory placode; Anterior neural fold; Neural crest; GANF", "note": "\u00a9 2014 International Society of Differentiation. Published by Elsevier B.V. \n\nAvailable online 26 September 2014. \n\nThis work was supported by DE16459 to MEB. We thank Elly Chow for their help in early portions of this work and Rusty Lansford for supplying quail embryos.\n\nAccepted Version - nihms802633.pdf
", "abstract": "The anterior neural fold (ANF) is the only region of the neural tube that does not produce neural crest cells. Instead, ANF cells contribute to the olfactory and lens placodes, as well as to the forebrain and epidermis. Here, we test the ability of the ANF to form neural crest by performing heterotopic transplantation experiments in the chick embryo. We find that, at the neurula stage (HH stage 7), the chick ANF retains the ability to form migrating neural crest cells when transplanted caudally to rostral hindbrain levels. This ability is gradually lost, such that by HH9, this tissue appears to no longer have the potential to form neural crest. In contrast to the ANF, hindbrain dorsal neural folds transplanted rostrally fail to contribute to the olfactory placode but instead continue to generate neural crest cells. The transcription factor GANF is expressed in the ANF and its morpholino-mediated knock-down expands the neural crest domain rostrally and results in the production of migratory cells emerging from the ANF; however, these cells fail to express the HNK1 neural crest marker, suggesting only partial conversion. Our results show that environmental factors can imbue the chick anterior neural folds to assume a neural crest cell fate via a mechanism that partially involves loss of GANF.", "date": "2014-09", "date_type": "published", "publication": "Differentiation", "volume": "88", "number": "2-3", "publisher": "Elsevier", "pagerange": "42-50", "id_number": "CaltechAUTHORS:20140929-091425513", "issn": "0301-4681", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140929-091425513", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1016/j.diff.2014.09.003", "pmcid": "PMC4957705", "primary_object": { "basename": "nihms802633.pdf", "url": "https://authors.library.caltech.edu/records/kceyz-hw842/files/nihms802633.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Ezin, Maxellende; Barembaum, Meyer; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0dc7w-gma92", "eprint_id": 49012, "eprint_status": "archive", "datestamp": "2023-08-20 01:56:46", "lastmod": "2023-10-17 20:36:34", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kwon-Seung-Hae", "name": { "family": "Kwon", "given": "Seung-Hae" } }, { "id": "Park-Ok-Kyu", "name": { "family": "Park", "given": "Ok Kyu" } }, { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" } }, { "id": "Kwak-Jina", "name": { "family": "Kwak", "given": "Jina" } }, { "id": "Hwang-Byung-Joon", "name": { "family": "Hwang", "given": "Byung Joon" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } } ] }, "title": "Bioinformatic Analysis of Nematode Migration-Associated Genes Identifies Novel Vertebrate Neural Crest Markers", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Kwon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived: January 18, 2014; Accepted: June 26, 2014; Published: July 22, 2014. \n\nFunding: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) (2011-0021845 to YK), by a 2013 Research Grant from Kangwon National University to YK, by Korea Basic Science Institute grant (T32611 to SK) and by HD037105 to MEB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. \n\nAcknowledgments: We thank Dr. Kye-Young Kim (NIH) for help with pathway analysis. \n\nThe authors have declared that no competing interests exist. \n\nAuthor Contributions: Conceived and designed the experiments: YK BJH. Performed the experiments: S-HK OKP SN JK BJH YK. Analyzed the data: S-HK OKP SN JK BJH YK. Contributed reagents/materials/analysis tools: YK MEB BJH. Wrote the paper: YK MEB BJH.\n\nPublished - journal.pone.0103024.pdf
", "abstract": "Neural crest cells are highly motile, yet a limited number of genes governing neural crest migration have been identified by conventional studies. To test the hypothesis that cell migration genes are likely to be conserved over large evolutionary distances and from diverse tissues, we searched for vertebrate homologs of genes important for migration of various cell types in the invertebrate nematode and examined their expression during vertebrate neural crest cell migration. Our systematic analysis utilized a combination of comparative genomic scanning, functional pathway analysis and gene expression profiling to uncover previously unidentified genes expressed by premigratory, emigrating and/or migrating neural crest cells. The results demonstrate that similar gene sets are expressed in migratory cell types across distant animals and different germ layers. Bioinformatics analysis of these factors revealed relationships between these genes within signaling pathways that may be important during neural crest cell migration.", "date": "2014-07-22", "date_type": "published", "publication": "PLoS ONE", "volume": "9", "number": "7", "publisher": "Public Library of Science", "pagerange": "Art. No. e103024", "id_number": "CaltechAUTHORS:20140828-084522656", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140828-084522656", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Research Foundation of Korea (NRF)", "grant_number": "2011-0021845" }, { "agency": "Kangwon National University" }, { "agency": "Korea Basic Science Institute", "grant_number": "T32611" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1371/journal.pone.0103024", "pmcid": "PMC4106859", "primary_object": { "basename": "journal.pone.0103024.pdf", "url": "https://authors.library.caltech.edu/records/0dc7w-gma92/files/journal.pone.0103024.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Kwon, Seung-Hae; Park, Ok Kyu; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6fxtw-b3c57", "eprint_id": 51583, "eprint_status": "archive", "datestamp": "2023-08-20 01:41:09", "lastmod": "2023-10-18 16:52:17", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Sir John Gurdon and his contributions to understanding fundamental principles in developmental biology", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2014 Elsevier B.V. \n\nAvailable online 31 October 2014.", "abstract": "I have had the pleasure of attending many conferences with Sir John Gurdon and have always loved to hear his insightful ideas, whether talking about \"reprogramming\", \"community effects\", or a \"ratchet\" mechanism for interpreting biological gradients. Throughout his long and distinguished career, John has made important discoveries and has remained a leading figure in developmental biology for over six decades, a remarkable feat in itself.", "date": "2014-07", "date_type": "published", "publication": "Differentiation", "volume": "88", "number": "1", "publisher": "Elsevier", "pagerange": "16", "id_number": "CaltechAUTHORS:20141111-125635101", "issn": "0301-4681", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141111-125635101", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.diff.2014.09.007", "resource_type": "article", "pub_year": "2014", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ep39d-k1w47", "eprint_id": 58408, "eprint_status": "archive", "datestamp": "2023-08-20 01:30:55", "lastmod": "2023-10-23 19:19:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Migrating into Genomics with the Neural Crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Marianne E. Bronner. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. \n\nReceived 25 December 2013; Accepted 18 March 2014; Published 22 June 2014.\n\nConflict of Interests: The author declares that there is no conflict of interests regarding the publication of this paper.\n\nPublished - 264069.pdf
", "abstract": "Neural crest cells are a fascinating embryonic cell type, unique to vertebrates, which arise within the central nervous system but\nemigrate soon after its formation and migrate to numerous and sometimes distant locations in the periphery. Following their\nmigratory phase, they differentiate into diverse derivatives ranging from peripheral neurons and glia to skin melanocytes and\ncraniofacial cartilage and bone.The molecular underpinnings underlying initial induction of prospective neural crest cells at the\nneural plate border to their migration and differentiation have been modeled in the form of a putative gene regulatory network.\nThis review describes experiments performed in my laboratory in the past few years aimed to test and elaborate this gene regulatory\nnetwork from both an embryonic and evolutionary perspective. The rapid advances in genomic technology in the last decade have\ngreatly expanded our knowledge of important transcriptional inputs and epigenetic influences on neural crest development. The\nresults reveal new players and new connections in the neural crest gene regulatory network and suggest that it has an ancient origin\nat the base of the vertebrate tree.", "date": "2014-06-22", "date_type": "published", "publication": "Advances in Biology", "volume": "2014", "publisher": "Hindawi Publishing Corporation", "pagerange": "Art. No. 264069", "id_number": "CaltechAUTHORS:20150622-131411504", "issn": "2356-6582", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150622-131411504", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1155/2014/264069", "primary_object": { "basename": "264069.pdf", "url": "https://authors.library.caltech.edu/records/ep39d-k1w47/files/264069.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5qhwc-awr60", "eprint_id": 44076, "eprint_status": "archive", "datestamp": "2023-08-20 01:05:18", "lastmod": "2023-10-26 00:09:47", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Wang-Kai", "name": { "family": "Wang", "given": "Kai" } }, { "id": "Milkie-D-E", "name": { "family": "Milkie", "given": "Daniel E." } }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Engerer-P", "name": { "family": "Engerer", "given": "Peter" } }, { "id": "Misgeld-T", "name": { "family": "Misgeld", "given": "Thomas" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Mumm-J", "name": { "family": "Mumm", "given": "Jeff" } }, { "id": "Betzig-E", "name": { "family": "Betzig", "given": "Eric" } } ] }, "title": "Rapid adaptive optical recovery of optimal resolution over large volumes", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Macmillan Publishers Limited.\n\nReceived 9 December 2013 ; Accepted 6 March 2014 ; Published online 13 April 2014.\n\nWe thank our colleagues N. Ji for many fruitful technical discussions and suggestion of the zebrafish system; P. Keller for the HRAS transgenic line; C. Yang, S. Narayan, M.B. Ahrens, M. Koyama, B. Lemon, K. McDole and P. Keller\nfor further guidance on zebrafish biology; J. Cox, M. Rose, A. Luck and J. Barber for zebrafish maintenance and breeding; and R. Kloss, B. Biddle and B. Bowers for machining services. We are grateful to R. K\u00f6ster (Technical University of Braunschweig) for providing the KalTA4 transactivator and X. Xie (Georgia Regents University) for assistance in generating corresponding transgenic Enhancer Trap lines. We also thank R. Kelsh (University of Bath) for the Sox10: eGFP line and U. Strahle (Karlsruhe Institute of Technology) for the Ngn:nRFP line. J.S.M. is supported by US National Institutes of Health (NIH) grants R21 MH083614 (NIMH) and R43 HD047089 (NICHD). M.E.B. is supported by NIH grant DE16459. T.M. acknowledges the financial support of the Center for Integrated Protein Sciences (EXC114 CIPS\nM) and of the Munich Cluster for Systems Neurology (EXC1010 SyNergy). P.E. was supported by DFG Research Training Group 1373. A.S. and P.E. acknowledge support from the Howard Hughes Medical Institute Janelia Farm visiting scientist program.\n\nAuthor Contributions:\nE.B. supervised the project; K.W. and E.B. conceived the idea; D.E.M., K.W. and E.B. developed the instrument control program; K.W. built the instrument and performed the experiments; A.S., P.E., T.M., M.E.B. and J.M. supplied zebrafish lines and guidance on live zebrafish imaging; K.W. and E.B. analyzed the data; E.B. wrote the paper with input from all co-authors.\n\nAccepted Version - nihms576781.pdf
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", "abstract": "Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.", "date": "2014-06", "date_type": "published", "publication": "Nature Methods", "volume": "11", "number": "6", "publisher": "Nature Publishing Group", "pagerange": "625-628", "id_number": "CaltechAUTHORS:20140303-060128636", "issn": "1548-7091", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140303-060128636", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Institute of Mental Health (NIMH)", "grant_number": "R21 MH083614" }, { "agency": "National Institute of Child Health and Human Development (NICHD)", "grant_number": "R43 HD047089" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Center for Integrated Protein Sciences", "grant_number": "EXC114 CIPS M" }, { "agency": "Munich Cluster for Systems Neurology", "grant_number": "EXC1010 SyNergy" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)" }, { "agency": "Howard Hughes Medical Institute (HHMI)" } ] }, "doi": "10.1038/nmeth.2925", "pmcid": "PMC4069208", "primary_object": { "basename": "nmeth.2925-sv3.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv3.mov" }, "related_objects": [ { "basename": "nmeth.2925-sv4.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv4.mov" }, { "basename": "nmeth.2925-sv5.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv5.mov" }, { "basename": "nmeth.2925-sv6.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv6.mov" }, { "basename": "nihms576781.pdf", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nihms576781.pdf" }, { "basename": "nmeth.2925-S1.pdf", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-S1.pdf" }, { "basename": "nmeth.2925-sv1.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv1.mov" }, { "basename": "nmeth.2925-sv2.mov", "url": "https://authors.library.caltech.edu/records/5qhwc-awr60/files/nmeth.2925-sv2.mov" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Wang, Kai; Milkie, Daniel E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5swh5-cfn02", "eprint_id": 43838, "eprint_status": "archive", "datestamp": "2023-08-22 12:38:42", "lastmod": "2023-10-25 23:55:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Maier-E-C", "name": { "family": "Maier", "given": "Esther C." } }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Alsina-B", "name": { "family": "Alsina", "given": "Berta" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Whitfield-T-T", "name": { "family": "Whitfield", "given": "Tanya T." } } ] }, "title": "Sensational placodes: Neurogenesis in the otic and olfactory systems", "ispublished": "pub", "full_text_status": "public", "keywords": "Otic; Olfactory; Neurogenesis; Hair cell; Sensory neuron", "note": "\u00a9 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license. Open Access funded by Biotechnology and Biological Sciences Research Council. \n\nAvailable online 6 February 2014. \n\nWe thank Leila Abbas and Lena Gunhaga for providing images or for permission to use images; Robert Kelsh for the Tg(\u20134.9sox10:eGFP) line and Yoshihiro Yoshihara, RIKEN BSI, and the National Bioresource Project of Japan for the Tg(TRPC24.5k:gap-Venus)/rw037 and Tg(OMP2k:lyn-mRFP)/rw035 lines that are featured in the figures. ECM was funded by a Marie Curie Intra European Fellowship (275978). This work was supported by the following grants: BFU2001-27006 to BA, NIH grants DE16459 and DC011577 to MEB, and a BBSRC project grant BB/J003050 to TTW.\n\nPublished - Maier_et_al.,_2014.pdf
Accepted Version - 1-s2.0-S0012160614000517-main.pdf
", "abstract": "For both the intricate morphogenetic lay out of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. In this review, we outline many of the proteins and pathways that play essential roles in the differentiation of otic and olfactory neurons and their integration into their non-neuronal support structures. In both cases, well-known signaling pathways together with region-specific factors transform thickened ectodermal placodes into complex sense organs containing numerous, diverse neuronal subtypes. Olfactory and otic placodes, in combination with migratory neural crest stem cells, generate highly specialized subtypes of neuronal cells that sense sound, position and movement in space, odors and pheromones throughout our lives.", "date": "2014-05-01", "date_type": "published", "publication": "Developmental Biology", "volume": "389", "number": "1", "publisher": "Elsevier", "pagerange": "50-67", "id_number": "CaltechAUTHORS:20140214-111259034", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140214-111259034", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Marie Curie Fellowship", "grant_number": "275978" }, { "agency": "Universitat Pompeu Fabra", "grant_number": "BFU2001-27006" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "NIH", "grant_number": "DC011577" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "BB/J003050" } ] }, "doi": "10.1016/j.ydbio.2014.01.023", "pmcid": "PMC3988839", "primary_object": { "basename": "1-s2.0-S0012160614000517-main.pdf", "url": "https://authors.library.caltech.edu/records/5swh5-cfn02/files/1-s2.0-S0012160614000517-main.pdf" }, "related_objects": [ { "basename": "Maier_et_al.,_2014.pdf", "url": "https://authors.library.caltech.edu/records/5swh5-cfn02/files/Maier_et_al.,_2014.pdf" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Maier, Esther C.; Saxena, Ankur; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/89yr6-57e56", "eprint_id": 43811, "eprint_status": "archive", "datestamp": "2023-08-22 12:32:49", "lastmod": "2023-10-25 23:54:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Betancur-P", "name": { "family": "Betancur", "given": "Paola" } }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Expression and function of transcription factor cMyb during cranial neural crest development", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Neural plate border; Gene regulatory network; cMyb; Transcription factor", "note": "\u00a9 2014 Published by Elsevier Ireland Ltd. \n\nReceived 30 October 2013. Received in revised form 26 January 2014. Accepted 29 January 2014. Available online 6 February 2014. \n\nThis work is supported by USPHS P01 HD037105.\n\nAccepted Version - nihms567184.pdf
", "abstract": "The transcription factor cMyb has well known functions in vertebrate hematopoiesis, but little was known about its distribution or function at early developmental stages. Here, we show that cMyb transcripts are present at the neural plate during gastrulation in chick embryos. cMyb expression then resolves to the cranial neural folds and is maintained in early migrating cranial neural crest cells during and after neurulation. Morpholino-mediated knock-down of cMyb reduces expression of Pax7 and Twist at the neural plate border, as well as reducing expression of neural crest specifier genes Snail2 and Sox10 and completely eliminating expression of Ets1. On the other hand, its loss results in abnormal maintenance of Zic1, but little or no effect on other neural crest specifier genes like FoxD3 or Sox9. These results place cMyb in a critical hierarchical position within the cranial neural crest cell gene regulatory network, likely directly inhibiting Zic1 and upstream of Ets1 and some, but not all, neural crest specifier genes.", "date": "2014-05", "date_type": "published", "publication": "Mechanisms of Development", "volume": "132", "publisher": "Elsevier", "pagerange": "38-43", "id_number": "CaltechAUTHORS:20140213-104955432", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140213-104955432", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P01 HD037105" } ] }, "doi": "10.1016/j.mod.2014.01.005", "pmcid": "PMC3987950", "primary_object": { "basename": "nihms567184.pdf", "url": "https://authors.library.caltech.edu/records/89yr6-57e56/files/nihms567184.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Betancur, Paola; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w7de0-y0824", "eprint_id": 42907, "eprint_status": "archive", "datestamp": "2023-08-19 23:26:35", "lastmod": "2023-10-25 23:08:32", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Kerosuo and Bronner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). This article was published online ahead of print in MBoC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E13-06-0327) \n\nReceived: Jun 19, 2013. Revised: Nov 7, 2013. Accepted: Nov 27, 2013. Published online before print December 4, 2013. \n\nThis work was supported by the Sigrid Juselius Foundation, the Foundations' Post doc Pool/Finnish Cultural Foundation, and the Ella and Georg Ehrnrooth Foundation (L.K.) and by National Institutes of Health Grant HD037105 to M.E.B.\n\nPublished - Mol._Biol._Cell-2014-Kerosuo-347-55.pdf
", "abstract": "Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle\u2013 and cell adhesion\u2013related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morpholino-mediated knockdown affects neural crest precursor survival, leading to reduction of neural plate border and neural crest specifier genes Msx-1, Pax7, FoxD3, and Sox10. Of interest, Miz1 loss also causes marked reduction of adhesion molecules (N-cadherin, cadherin6B, and \u03b11-catenin) with a concomitant increase of E-cadherin in the neural folds, likely leading to delayed and decreased neural crest emigration. Conversely, Miz1 overexpression results in up-regulation of cadherin6B and FoxD3 expression in the neural folds/neural tube, leading to premature neural crest emigration and increased number of migratory crest cells. Although Miz1 loss effects cell survival and proliferation throughout the neural plate, the neural progenitor marker Sox2 was unaffected, suggesting a neural crest\u2013selective effect. The results suggest that Miz1 is important not only for survival of neural crest precursors, but also for maintenance of integrity of the neural folds and tube, via correct formation of the apical adhesion complex therein.", "date": "2014-02-01", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "25", "number": "3", "publisher": "American Society for Cell Biology", "pagerange": "347-355", "id_number": "CaltechAUTHORS:20131209-133718257", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20131209-133718257", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Sigrid Juselius Foundation" }, { "agency": "Finnish Cultural Foundation" }, { "agency": "Ella och Georg Ehrnrooth's Foundation" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1091/mbc.E13-06-0327", "pmcid": "PMC3907275", "primary_object": { "basename": "Mol._Biol._Cell-2014-Kerosuo-347-55.pdf", "url": "https://authors.library.caltech.edu/records/w7de0-y0824/files/Mol._Biol._Cell-2014-Kerosuo-347-55.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Kerosuo, Laura and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b58e2-63n02", "eprint_id": 43355, "eprint_status": "archive", "datestamp": "2023-08-19 23:20:05", "lastmod": "2023-10-25 23:29:32", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Tan-Cabugao-J", "name": { "family": "Tan-Cabugao", "given": "Joanne" } }, { "id": "Antoshechkin-I-A", "name": { "family": "Antoshechkin", "given": "Igor" }, "orcid": "0000-0002-9934-3040" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Transcriptome analysis reveals novel players in the cranial neural crest gene regulatory network", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Sim\u00f5es-Costa et al. Published by Cold Spring Harbor Laboratory Press. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. \n\nReceived May 28, 2013; accepted in revised form November 6, 2013; Published in Advance January 3, 2014. \n\nWe thank Diana Perez, Janice Grimm, and Rochelle Diamond for their excellent cell-sorting assistance. The Caltech Flow Cytometry Cell Sorting Facility is supported by funds from the Beckman Institute at Caltech. We thank Dr. Max Ezin for her help with live time-lapse imaging of enhancer activity, and Mike Stone and Brian Jun for technical assistance. This work was supported by NIH HD037105 and DE16459 (to M.E.B.) and the Pew Fellows Program in the Biomedical Sciences (to M.S.-C.), and a Caltech Cell Center fellowship from the Moore Foundation (to M.S.-C.).\n\nPublished - Genome_Res.-2014-Sim\u00f5es-Costa-281-90.pdf
Published - Simoes-Costa_2014.pdf
Supplemental Material - Supplemental_Figure1.jpg
Supplemental Material - Supplemental_Legends.docx
Supplemental Material - Supplemental_TSS_movie_vert.avi
Supplemental Material - Supplemental_Table1.xlsx
Supplemental Material - Supplemental_Table2.xlsx
Supplemental Material - Supplemental_Table3.xlsx
Supplemental Material - Supplemental_Table4.xlsx
Supplemental Material - Supplemental_Table5.xlsx
", "abstract": "The neural crest is an embryonic stem cell population that gives rise to a multitude of derivatives. In particular, the cranial neural crest (CNC) is unique in its ability to contribute to both facial skeleton and peripheral ganglia. To gain further insight into the molecular underpinnings that distinguish the CNC from other embryonic tissues, we have utilized a CNC-specific enhancer as a tool to isolate a pure, region-specific NC subpopulation for transcriptional profiling. The resulting data set reveals previously unknown transcription factors and signaling pathways that may influence the CNC's ability to migrate and/or differentiate into unique derivatives. To elaborate on the CNC gene regulatory network, we evaluated the effects of knocking down known neural plate border genes and early neural crest specifier genes on selected neural crest-enriched transcripts. The results suggest that ETS1 and SOX9 may act as pan-neural crest regulators of the migratory CNC. Taken together, our analysis provides unprecedented characterization of the migratory CNC transcriptome and identifies new links in the gene regulatory network responsible for development of this critical cell population.", "date": "2014-02", "date_type": "published", "publication": "Genome Research", "volume": "24", "number": "2", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "281-290", "id_number": "CaltechAUTHORS:20140114-095636472", "issn": "1088-9051", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140114-095636472", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Caltech Beckman Institute" }, { "agency": "Pew Fellows Program in the Biomedical Sciences" }, { "agency": "Gordon and Betty Moore Foundation" } ] }, "doi": "10.1101/gr.161182.113", "pmcid": "PMC3912418", "primary_object": { "basename": "Simoes-Costa_2014.pdf", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Simoes-Costa_2014.pdf" }, "related_objects": [ { "basename": "Supplemental_Figure1.jpg", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Figure1.jpg" }, { "basename": "Supplemental_Legends.docx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Legends.docx" }, { "basename": "Supplemental_TSS_movie_vert.avi", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_TSS_movie_vert.avi" }, { "basename": "Supplemental_Table3.xlsx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Table3.xlsx" }, { "basename": "Supplemental_Table4.xlsx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Table4.xlsx" }, { "basename": "Genome_Res.-2014-Sim\u00f5es-Costa-281-90.pdf", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Genome_Res.-2014-Sim\u00f5es-Costa-281-90.pdf" }, { "basename": "Supplemental_Table1.xlsx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Table1.xlsx" }, { "basename": "Supplemental_Table2.xlsx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Table2.xlsx" }, { "basename": "Supplemental_Table5.xlsx", "url": "https://authors.library.caltech.edu/records/b58e2-63n02/files/Supplemental_Table5.xlsx" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Sim\u00f5es-Costa, Marcos; Tan-Cabugao, Joanne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xact3-g7v75", "eprint_id": 44227, "eprint_status": "archive", "datestamp": "2023-08-22 11:36:42", "lastmod": "2023-10-26 00:18:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hochgreb-H\u00e4gele-T", "name": { "family": "Hochgreb-H\u00e4gele", "given": "Tatiana" } }, { "id": "Koo-Daniel-E-S", "name": { "family": "Koo", "given": "Daniel E. S." } }, { "id": "Das-N-M", "name": { "family": "Das", "given": "Neha M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Zebrafish Stem/Progenitor Factor msi2b Exhibits Two Phases of Activity Mediated by Different Splice Variants", "ispublished": "pub", "full_text_status": "public", "keywords": "Musashi; zebrafish; neuronal progenitor; differentiating", "note": "\u00a9 2013 AlphaMed Press. Received May 17, 2013; accepted for publication September 18, 2013; first published online in STEM CELLS EXPRESS October 29, 2013. \n\nWe thank Dr. Tatjana Sauka-Spengler for useful discussions, Drs. Marcos Sim~oes-Costa and Sujata Bhattacharyya for comments on the manuscript, and Drs. Le Trinh and Scott Fraser for helpful advice. We also thank Leigh Ann Fletcher and Kwok Su for fish care, Alice Plein and Ilana Solomon for technical support, Dr. Bruce Appel for sharing the Tg(olig2:dsred) line, Dr. Uwe Strahle for the Tg(ngn1:nRFP) line, and Dr. Yoshihiro Yoshihara, RIKEN BSI, and National Bioresource Project of Japan for the Tg(omp:RFG) and Tg(trpc2:Venus) lines. This work was supported by Pew Latin American Fellowship in the Biomedical Sciences and by California Institute for Regenerative Medicine Training Grant (T2\u201300006; to T.H.-H.). This work was supported by Grants HD037105 and HG004071 from NIH (to M.E.B). The authors indicate no potential conflicts of interest. \n\nAuthor Contributions: T.H.-H.: designed and performed experiments, analyzed data, and wrote the manuscript; D.E.S.K. and N.M.D.: performed experiments, analyzed data, and edited the manuscript; M.E. B.: supervised the work including helping with experimental design, data, analysis, and manuscript preparation.\n\nAccepted Version - nihms-541146.pdf
", "abstract": "The Musashi (Msi) family of RNA-binding proteins is important in stem and differentiating cells in many species. Here, we present a zebrafish gene/protein trap line gt(msi2b-citrine)(ct) (57) (a) that expresses a Citrine fusion protein with endogenous Msi2b. Our results reveal two phases of Msi2b expression: ubiquitous expression in progenitor cells in the early embryo and later, tissue-specific expression in differentiating cells in the olfactory organ, pineal gland, and subpopulations of neurons in the central nervous system (CNS). Interestingly, this division between early and late phases is paralleled by differential expression of msi2b alternative splicing products. Whereas the full-length and long variant v3 Msi2b predominate at early stages, the later expression of variants in differentiating tissues appears to be tissue specific. Using the gt(msi2b-citrine)(ct) (57) (a), we characterized tissue-specific expression of Msi2b with cellular resolution in subsets of differentiating cells in the olfactory organ, pineal gland, CNS, and ventral neural tube. By performing transcription activator-like effectors nuclease-mediated biallelic genome editing or morpholino knockdown of Msi2b in zebrafish, our results show that early inactivation of Msi2b results in severe embryonic defects including hypertrophy of the ventricles and shortening of the body, consistent with an important role in cell proliferation and survival. Moreover, specific inactivation of Msi2b full-length indicates that this species is essential for the early role of Msi2b. This line provides a valuable tool both for live imaging of the endogenous Msi2b at subcellular resolution and manipulation of Msi2b-expressing cells.", "date": "2014-02", "date_type": "published", "publication": "Stem Cells", "volume": "32", "number": "2", "publisher": "AlphaMed Press", "pagerange": "558-571", "id_number": "CaltechAUTHORS:20140310-140613290", "issn": "1549-4918", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140310-140613290", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pew Latin American Fellowship in the Biomedical Sciences" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "T2-00006" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "HG004071" } ] }, "doi": "10.1002/stem.1583", "pmcid": "PMC3901373", "primary_object": { "basename": "nihms-541146.pdf", "url": "https://authors.library.caltech.edu/records/xact3-g7v75/files/nihms-541146.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Hochgreb-H\u00e4gele, Tatiana; Koo, Daniel E. S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/v7v1y-7zy91", "eprint_id": 43877, "eprint_status": "archive", "datestamp": "2023-08-22 11:29:18", "lastmod": "2023-10-25 23:57:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Modrell-M-S", "name": { "family": "Modrell", "given": "Melinda S." } }, { "id": "Hockman-D", "name": { "family": "Hockman", "given": "Dorit" }, "orcid": "0000-0003-2613-6216" }, { "id": "Uy-B", "name": { "family": "Uy", "given": "Benjamin" } }, { "id": "Buckley-D", "name": { "family": "Buckley", "given": "David" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } } ] }, "title": "A fate-map for cranial sensory ganglia in the sea lamprey", "ispublished": "pub", "full_text_status": "public", "keywords": "Placodes; Neural crest; Ophthalmic trigeminal; Profundal; Maxillomandibular trigeminal; Pax3/7", "note": "\u00a9 2013 The Authors. Published by Elsevier Inc. Attribution 3.0 Unported (CC BY 3.0) \n\nReceived 26 April 2013. Received in revised form 17 October 2013. Accepted 21 October 2013. Available online 26 October 2013. \n\nThis work was funded by the Biotechnology & Biological Sciences Research Council (Grant BB/F00818X/1 to C.V.H.B), the Wellcome Trust (Ph.D. Studentship 086804/Z/08/Z to C.V.H.B. and D.H.) and the National Institutes of Health (Grants DE017911 and DE16459 to M.E.B). We thank Yasunori Murakami and Shigeru Kuratani for their advice on the identification of the cranial ganglia.\n\nPublished - Modrell_2014p405.pdf
Supplemental Material - mmc1.doc
", "abstract": "Cranial neurogenic placodes and the neural crest make essential contributions to key adult characteristics of all vertebrates, including the paired peripheral sense organs and craniofacial skeleton. Neurogenic placode development has been extensively characterized in representative jawed vertebrates (gnathostomes) but not in jawless fishes (agnathans). Here, we use in vivo lineage tracing with DiI, together with neuronal differentiation markers, to establish the first detailed fate-map for placode-derived sensory neurons in a jawless fish, the sea lamprey Petromyzon marinus, and to confirm that neural crest cells in the lamprey contribute to the cranial sensory ganglia. We also show that a pan-Pax3/7 antibody labels ophthalmic trigeminal (opV, profundal) placode-derived but not maxillomandibular trigeminal (mmV) placode-derived neurons, mirroring the expression of gnathostome Pax3 and suggesting that Pax3 (and its single Pax3/7 lamprey ortholog) is a pan-vertebrate marker for opV placode-derived neurons. Unexpectedly, however, our data reveal that mmV neuron precursors are located in two separate domains at neurula stages, with opV neuron precursors sandwiched between them. The different branches of the mmV nerve are not comparable between lampreys and gnatho-stomes, and spatial segregation of mmV neuron precursor territories may be a derived feature of lampreys. Nevertheless, maxillary and mandibular neurons are spatially segregated within gnathostome mmV ganglia, suggesting that a more detailed investigation of gnathostome mmV placode development would be worthwhile. Overall, however, our results highlight the conservation of cranial peripheral sensory nervous system development across vertebrates, yielding insight into ancestral vertebrate traits.", "date": "2014-01-15", "date_type": "published", "publication": "Developmental Biology", "volume": "385", "number": "2", "publisher": "Elsevier", "pagerange": "405-416", "id_number": "CaltechAUTHORS:20140219-143421747", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140219-143421747", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "BB/F00818X/1" }, { "agency": "Wellcome Trust", "grant_number": "086804/Z/08/Z" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1016/j.ydbio.2013.10.021", "pmcid": "PMC3928997", "primary_object": { "basename": "Modrell_2014p405.pdf", "url": "https://authors.library.caltech.edu/records/v7v1y-7zy91/files/Modrell_2014p405.pdf" }, "related_objects": [ { "basename": "mmc1.doc", "url": "https://authors.library.caltech.edu/records/v7v1y-7zy91/files/mmc1.doc" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Modrell, Melinda S.; Hockman, Dorit; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8ez0k-f2k31", "eprint_id": 43832, "eprint_status": "archive", "datestamp": "2023-08-19 23:04:02", "lastmod": "2023-10-25 23:55:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Elgar-G", "name": { "family": "Elgar", "given": "Greg" } } ] }, "title": "A Reporter Assay in Lamprey Embryos Reveals Both Functional Conservation and Elaboration of Vertebrate Enhancers", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2014 Parker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.\n\nReceived: August 19, 2013; Accepted: December 5, 2013; Published: January 9, 2014.\n\nThis work was supported by MRC core funding (U117597141) to GE and grants GM090049 and DE017911 to MEB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.\n\n\nWe thank Natalya Nikitina, Benjamin Uy, Melinda Modrell and Marcos Simoes-Costa for advice and assistance on lamprey husbandry. We thank Heather Callaway for zebrafish maintenance.\n\nAuthor Contributions:\nConceived and designed the experiments: HP GE TSS MB. Performed the\nexperiments: HP TSS. Analyzed the data: HP. Contributed reagents/\nmaterials/analysis tools: GE MB. Wrote the paper: HP GE.\n\nPublished - journal.pone.0085492.pdf
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", "abstract": "The sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the vertebrate taxa. The identification of these regions makes it possible to address how changes at the genomic level have led to changes in developmental gene regulatory networks and ultimately to the evolution of morphological diversity. Comparative genomics approaches using sea lamprey have already predicted a number of such regulatory elements in the lamprey genome. Functional characterisation of these sequences and other similar elements requires efficient reporter assays in lamprey. In this report, we describe the development of a transient transgenesis method for lamprey embryos. Focusing on conserved non-coding elements (CNEs), we use this method to investigate their functional conservation across the vertebrate subphylum. We find instances of both functional conservation and lineage-specific functional evolution of CNEs across vertebrates, emphasising the utility of functionally testing homologous CNEs in their host species.", "date": "2014-01-09", "date_type": "published", "publication": "PLoS ONE", "volume": "9", "number": "1", "publisher": "Public Library of Science", "pagerange": "Art. No. e85492", "id_number": "CaltechAUTHORS:20140214-093316457", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140214-093316457", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Medical Research Council (MRC)", "grant_number": "U117597141" }, { "agency": "NIH", "grant_number": "GM090049" }, { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1371/journal.pone.0085492", "pmcid": "PMC3887057", "primary_object": { "basename": "journal.pone.0085492.pdf", "url": "https://authors.library.caltech.edu/records/8ez0k-f2k31/files/journal.pone.0085492.pdf" }, "related_objects": [ { "basename": "journal.pone.0085492.s001.pdf", "url": "https://authors.library.caltech.edu/records/8ez0k-f2k31/files/journal.pone.0085492.s001.pdf" }, { "basename": "journal.pone.0085492.s002.pdf", "url": "https://authors.library.caltech.edu/records/8ez0k-f2k31/files/journal.pone.0085492.s002.pdf" }, { "basename": "journal.pone.0085492.s003.tif", "url": "https://authors.library.caltech.edu/records/8ez0k-f2k31/files/journal.pone.0085492.s003.tif" }, { "basename": "journal.pone.0085492.s004.doc", "url": "https://authors.library.caltech.edu/records/8ez0k-f2k31/files/journal.pone.0085492.s004.doc" } ], "resource_type": "article", "pub_year": "2014", "author_list": "Parker, Hugo J.; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9shse-2c829", "eprint_id": 42843, "eprint_status": "archive", "datestamp": "2023-08-19 22:59:31", "lastmod": "2023-10-25 23:05:13", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Letter from new DB Editor-in-Chief", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2013 Published by Elsevier Inc.", "abstract": "At the onset of 2014, I am honored to assume the position of Editor-in-Chief of Developmental Biology, the official journal of the Society for Developmental Biology, and published by Elsevier. First and foremost, I would like to take this opportunity to thank the out-going Editor-in-Chief, Robb Krumlauf, for his wonderful leadership and outstanding contributions to the journal over the past nine years. Having served as an editor under his guidance, I have learned much from his excellent example and hope to continue on the positive trajectory he has put in place. In dealing with authors and editors alike, he handles manuscripts with fairness, patience, dedication and attention to detail. I also thank my fellow editors for their commitment and devotion to the journal and look forward to continuing interactions with them on the editorial board. Developmental Biology is run entirely by active scientists and for scientists, and we thank them for dedicating their valuable time and energy to efficient operation of our society's Journal.", "date": "2014-01-01", "date_type": "published", "publication": "Developmental Biology", "volume": "385", "number": "1", "publisher": "Elsevier", "pagerange": "1-1", "id_number": "CaltechAUTHORS:20131205-080030823", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20131205-080030823", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2013.10.028", "resource_type": "article", "pub_year": "2014", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ne0f2-zvf23", "eprint_id": 44034, "eprint_status": "archive", "datestamp": "2023-08-22 11:16:49", "lastmod": "2023-10-26 00:07:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Green-S-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The lamprey: A jawless vertebrate model system for examining origin of the neural crest and other vertebrate traits", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Lamprey; Neural crest derivatives; Vertebrate evolution", "note": "\u00a9 2014 International Society of Differentiation. Published by Elsevier B.V. \n\nAvailable online 20 February 2014. \n\nWe would like to thank C. V. Baker and members of the Bronner Laboratory for helpful discussions. This work was supported by the NIH (R01 NS086907).\n\nAccepted Version - nihms567143.pdf
", "abstract": "Lampreys are a group of jawless fishes that serve as an important point of comparison for studies of vertebrate evolution. Lampreys and hagfishes are agnathan fishes, the cyclostomes, which sit at a crucial phylogenetic position as the only living sister group of the jawed vertebrates. Comparisons between cyclostomes and jawed vertebrates can help identify shared derived (i.e. synapomorphic) traits that might have been inherited from ancestral early vertebrates, if unlikely to have arisen convergently by chance. One example of a uniquely vertebrate trait is the neural crest, an embryonic tissue that produces many cell types crucial to vertebrate features, such as the craniofacial skeleton, pigmentation of the skin, and much of the peripheral nervous system (Gans and Northcutt, 1983). Invertebrate chordates arguably lack unambiguous neural crest homologs, yet have cells with some similarities, making comparisons with lampreys and jawed vertebrates essential for inferring characteristics of development in early vertebrates, and how they may have evolved from nonvertebrate chordates. Here we review recent research on cyclostome neural crest development, including research on lamprey gene regulatory networks and differentiated neural crest fates.", "date": "2014-01", "date_type": "published", "publication": "Differentiation", "volume": "87", "number": "1-2", "publisher": "Elsevier", "pagerange": "44-51", "id_number": "CaltechAUTHORS:20140227-105237117", "issn": "0301-4681", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140227-105237117", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 NS086907" } ] }, "doi": "10.1016/j.diff.2014.02.001", "pmcid": "PMC3995830", "primary_object": { "basename": "nihms567143.pdf", "url": "https://authors.library.caltech.edu/records/ne0f2-zvf23/files/nihms567143.pdf" }, "resource_type": "article", "pub_year": "2014", "author_list": "Green, Stephen A. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3x1n8-9rk66", "eprint_id": 42918, "eprint_status": "archive", "datestamp": "2023-08-19 22:32:27", "lastmod": "2023-10-25 23:08:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rogers-C-D", "name": { "family": "Rogers", "given": "Crystal D." }, "orcid": "0000-0002-9549-1089" }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Rogers et al. This article is distributed under the terms of an Attribution\u2013Noncommercial\u2013Share Alike\u2013No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). \n\nSubmitted: 9 May 2013; Accepted: 4 November 2013; Published December 2, 2013. \n\nWe would like to thank Tatjana Sauka-Spengler and the Bronner laboratory for helpful discussions. We would also like to thank the Caltech Biological Imaging\ncenter where we performed the live-imaging experiments and the Mertz Lab at\nthe University of Wisconsin at Madison for the gift of human Zeb2 DNA.\nThis work was supported by National Institutes of Health grant HD037105\n(to M.E. Bronner) and a National Institutes of Health minority supplement P01\nHD037105 (to C.D. Rogers).\n\nPublished - Rogers_2013.pdf
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Supplemental Material - JCB_201305050_sm.pdf
", "abstract": "The neural crest, an embryonic stem cell population,\ninitially resides within the dorsal neural tube but subsequently\nundergoes an epithelial-to-mesenchymal\ntransition (EMT) to commence migration. Although neural\ncrest and cancer EMTs are morphologically similar, little is\nknown regarding conservation of their underlying molecular\nmechanisms. We report that Sip1, which is involved\nin cancer EMT, plays a critical role in promoting the neural\ncrest cell transition to a mesenchymal state. Sip1 transcripts\nare expressed in premigratory/migrating crest\ncells. After Sip1 loss, the neural crest specifier gene FoxD3\nwas abnormally retained in the dorsal neuroepithelium,\nwhereas Sox10, which is normally required for emigration,\nwas diminished. Subsequently, clumps of adherent\nneural crest cells remained adjacent to the neural tube and\naberrantly expressed E-cadherin while lacking N-cadherin.\nThese findings demonstrate two distinct phases of neural\ncrest EMT, detachment and mesenchymalization, with the\nlatter involving a novel requirement for Sip1 in regulation\nof cadherin expression during completion of neural\ncrest EMT.", "date": "2013-12-09", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "203", "number": "5", "publisher": "Rockefeller University Press", "pagerange": "835-847", "id_number": "CaltechAUTHORS:20131210-100609180", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20131210-100609180", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "P01 HD037105" } ] }, "doi": "10.1083/jcb.201305050", "pmcid": "PMC3857483", "primary_object": { "basename": "JCB_201305050_V6.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V6.mov" }, "related_objects": [ { "basename": "JCB_201305050_sm.pdf", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_sm.pdf" }, { "basename": "Rogers_2013.pdf", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/Rogers_2013.pdf" }, { "basename": "JCB_201305050_V1.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V1.mov" }, { "basename": "JCB_201305050_V2.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V2.mov" }, { "basename": "JCB_201305050_V3.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V3.mov" }, { "basename": "JCB_201305050_V4.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V4.mov" }, { "basename": "JCB_201305050_V5.mov", "url": "https://authors.library.caltech.edu/records/3x1n8-9rk66/files/JCB_201305050_V5.mov" } ], "resource_type": "article", "pub_year": "2013", "author_list": "Rogers, Crystal D.; Saxena, Ankur; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7gzyd-1z805", "eprint_id": 45419, "eprint_status": "archive", "datestamp": "2023-08-22 11:01:45", "lastmod": "2023-10-26 17:59:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Clonal analyses in the anterior pre-placodal region: implications for the early lineage bias of placodal progenitors", "ispublished": "pub", "full_text_status": "public", "keywords": "placode; olfactory; lens; pre-placodal region; lineage", "note": "\u00a9 2013 UBC Press. \n\nAccepted: 3 July 2013. Final, author-corrected PDF published online: 4 December 2013. \n\nWe are extremely grateful to Dr. Scott E. Fraser for lending his expertise in teaching SB the technique of intracellular injections. We would also like to thank Drs. Tatiana Hochgreb and Marcos Sim\u00f5es-Costa for their insightful comments and helpful discussions. This work was supported by R01 DE16459 to MEB.\n\nAccepted Version - nihms585091.pdf
", "abstract": "Cranial ectodermal placodes, a vertebrate innovation, contribute to the adenohypophysis and peripheral nervous system of the head, including the paired sense organs (eyes, nose, ears) and sensory ganglia of the Vth, VIIth, IXth and Xth cranial nerves. Fate-maps of groups of cells in amphibians, teleosts and amniotes have demonstrated that all placodes have a common origin in a horseshoe shaped territory, known as the preplacodal region (PPR), which surrounds the presumptive neural plate of the late gastrula/early neurula stage embryo. Given the extensive regional overlap of progenitors for different placodes in the chick embryo, it has been a matter of debate as to whether individual cells in the PPR are truly multipotent progenitors, with regard to placodal identity, or rather are lineage-biased or restricted to a specific placodal type prior to overt differentiation. Utilizing clonal analyses in vivo, we demonstrate here that the anterior PPR comprises some precursors that contribute either to the olfactory or lens placode well before they are spatially segregated or committed to either of these placodal fates. This suggests that lineage bias towards a specific placodal fate may coincide with induction of the PPR.", "date": "2013-12", "date_type": "published", "publication": "International Journal of Developmental Biology", "volume": "57", "number": "9-10", "publisher": "University of the Basque Country Press", "pagerange": "753-757", "id_number": "CaltechAUTHORS:20140501-111631382", "issn": "0214-6282", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140501-111631382", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE16459" } ] }, "doi": "10.1387/ijdb.130155mb", "pmcid": "PMC4041203", "primary_object": { "basename": "nihms585091.pdf", "url": "https://authors.library.caltech.edu/records/7gzyd-1z805/files/nihms585091.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Bhattacharyya, Sujata and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dsbfp-bvq25", "eprint_id": 40987, "eprint_status": "archive", "datestamp": "2023-08-22 10:36:48", "lastmod": "2023-10-24 23:21:26", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Identification and dissection of a key enhancer mediating cranial neural crest specific expression of transcription factor, Ets-1", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Ets-1; Enhancer; TFAP2; Pax7; Sox9", "note": "\u00a9 2013 Published by Elsevier Inc. \n\nReceived 1 April 2013; Received in revised form 9 August 2013; Accepted 12 August 2013; Available online 19 August 2013. \n\nWe would like to thank Tatjana Sauka-Spengler for her valuable advice and Marcos Sim\u00f5es-Costa for his comments in writing this paper. This work was supported by USPHS P01 HD037105 and DE16459.\n\nAccepted Version - nihms517712.pdf
", "abstract": "Neural crest cells form diverse derivatives that vary according to their level of origin along the body axis, with only cranial neural crest cells contributing to facial skeleton. Interestingly, the transcription factor Ets-1 is uniquely expressed in cranial but not trunk neural crest, where it functions as a direct input into neural crest specifier genes, Sox10 and FoxD3. We have isolated and interrogated a cis-regulatory element, conserved between birds and mammals, that drives reporter expression in a manner that recapitulates that of endogenous Ets-1 expression in the neural crest. Within a minimal Ets-1 enhancer region, mutation of putative binding sites for SoxE, homeobox, Ets, TFAP2 or Fox proteins results in loss or reduction of neural crest enhancer activity. Morpholino-mediated loss-of-function experiments show that Sox9, Pax7, Msx1/2, Ets-1, TFAP2A and FoxD3, all are required for enhancer activity. In contrast, mutation of a putative cMyc/E-box sequence augments reporter expression, consistent with this being a repressor binding site. Taken together, these results uncover new inputs into Ets-1, revealing critical links in the cranial neural crest gene regulatory network.", "date": "2013-10-15", "date_type": "published", "publication": "Developmental Biology", "volume": "382", "number": "2", "publisher": "Elsevier", "pagerange": "567-575", "id_number": "CaltechAUTHORS:20130829-092042694", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130829-092042694", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P01 HD037105" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1016/j.ydbio.2013.08.009", "pmcid": "PMC3872135", "primary_object": { "basename": "nihms517712.pdf", "url": "https://authors.library.caltech.edu/records/dsbfp-bvq25/files/nihms517712.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Barembaum, Meyer and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zmn37-he828", "eprint_id": 40331, "eprint_status": "archive", "datestamp": "2023-08-19 20:53:52", "lastmod": "2023-10-24 17:20:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Chao-Jennifer-R", "name": { "family": "Chao", "given": "Jennifer R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } } ] }, "title": "Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Mary Ann Liebert, Inc. \n\nReceived for publication January 5, 2013; Accepted after revision March 4, 2013; Prepublished on Liebert Instant Online March 6, 2013. \n\nThis work was supported by the National Eye Institute EY022158 (P.Y.L.), the National Institute of Health DE16459 (M.E.B.), the National Eye Institute K08EY019714 ( J.R.C.), and grants from Fight for Sight, the Knights Templar Eye Foundation, and an Unrestricted Grant from Research to Prevent Blindness ( J.R.C.). A portion of this work was previously presented in two abstracts:\n1. JR Chao, M Bronner-Fraser, and PY Lwigale. Stem-cell\nproperties of human corneal keratocytes. ARVO Meeting\nAbstracts, April 11, 2009, 50:2049.\n2. JR Chao, M Bronner-Fraser, and PY Lwigale. Corneal\nplasticity: characterization of the multipotentiality of human\nkeratocytes. ARVO Meeting Abstracts, April 11, 2008,\n49:4812.\n\nPublished - Chao_2013p2186.pdf
", "abstract": "The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and corneal endothelium. However, they failed to form neurons despite their presence in the condensing trigeminal ganglion. These results show that HFKs retain the ability to differentiate into some neural crest-derived tissues. Their ability to respond to embryonic cues and generate corneal endothelium and stromal keratocytes provides a basis for understanding the feasibility of creating specialized cells for possible use in regenerative medicine.", "date": "2013-08", "date_type": "published", "publication": "Stem Cells and Development", "volume": "22", "number": "15", "publisher": "Mary Ann Liebert", "pagerange": "2186-2195", "id_number": "CaltechAUTHORS:20130816-095950105", "issn": "1547-3287", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130816-095950105", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Eye Institute", "grant_number": "EY022158" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "National Eye Institute", "grant_number": "K08EY019714" }, { "agency": "Fight for Sight" }, { "agency": "Knights Templar Eye Foundation" }, { "agency": "Research to Prevent Blindness" } ] }, "doi": "10.1089/scd.2013.0011", "pmcid": "PMC3715791", "primary_object": { "basename": "Chao_2013p2186.pdf", "url": "https://authors.library.caltech.edu/records/zmn37-he828/files/Chao_2013p2186.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Chao, Jennifer R.; Bronner, Marianne E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9v261-nfb80", "eprint_id": 39313, "eprint_status": "archive", "datestamp": "2023-08-22 09:49:09", "lastmod": "2023-10-24 16:40:27", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hochgreb-H\u00e4gele-T", "name": { "family": "Hochgreb-H\u00e4gele", "given": "Tatiana" } }, { "id": "Yin-Chunyue", "name": { "family": "Yin", "given": "Chunyue" } }, { "id": "Koo-Daniel-E-S", "name": { "family": "Koo", "given": "Daniel E. S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Stainier-D-Y-R", "name": { "family": "Stainier", "given": "Didier Y. R." } } ] }, "title": "Laminin \u03b21a controls distinct steps during the establishment of digestive organ laterality", "ispublished": "pub", "full_text_status": "public", "keywords": "Asymmetry; Laminin; Organogenesis", "note": "\u00a9 2013 The Company of Biologists Ltd. \n\nAccepted April 22, 2013. \n\nWe thank Elke Ober, Holly Field, Heather Verkade and other members of the ENU screen team, as well as Chantilly (Munson) Apollon for sharing reagents and expertise in LR organ asymmetry. We thank Mike Parsons for the grumpy allele and Thai Truong for assistance with settings for live imaging of the KV flow. Thanks to Zhaoxia Sun for sharing before publication her protocol for the analysis of KV flow, and to Pablo Ote\u00edza and Hiroaki Ishikawa for advice. \n\nFunding: T.H.-H. was supported by a Pew Latin American Fellowship in the Biomedical Sciences and by a California Institute for Regenerative Medicine Training Grant [T2-00006]. C.Y. is supported by an National Institutes of Health (NIH) K99 Award [AA020514], a University of California at San Francisco Liver Center Pilot/Feasibility Award [NIH P30DK026743] and the Cincinnati Children's Hospital Research Foundation. This work was supported in part by grants from the NIH [P50 HG004071 to M.E.B., R01DK060322 to D.Y.R.S.] and from the Packard Foundation (to D.Y.R.S.). Deposited in PMC for release after 12 months. \n\nAuthor contributions: T.H.-H. and C.Y. designed and performed experiments, analyzed data and wrote the manuscript. D.E.S.K. performed experiments, analyzed data and edited the manuscript. M.E.B. and D.Y.R.S. supervised the work, including helping with experimental design, data, analysis and manuscript preparation.\n\nPublished - 2734.full.pdf
", "abstract": "Visceral organs, including the liver and pancreas, adopt asymmetric positions to ensure proper function. Yet the molecular and cellular mechanisms controlling organ laterality are not well understood. We identified a mutation affecting zebrafish laminin \u03b21a (lamb1a) that disrupts left-right asymmetry of the liver and pancreas. In these mutants, the liver spans the midline and the ventral pancreatic bud remains split into bilateral structures. We show that lamb1a regulates asymmetric left-right gene expression in the lateral plate mesoderm (LPM). In particular, lamb1a functions in Kupffer's vesicle (KV), a ciliated organ analogous to the mouse node, to control the length and function of the KV cilia. Later during gut-looping stages, dynamic expression of Lamb1a is required for the bilayered organization and asymmetric migration of the LPM. Loss of Lamb1a function also results in aberrant protrusion of LPM cells into the gut. Collectively, our results provide cellular and molecular mechanisms by which extracellular matrix proteins regulate left-right organ morphogenesis.", "date": "2013-07-01", "date_type": "published", "publication": "Development", "volume": "140", "number": "13", "publisher": "Company of Biologists", "pagerange": "2734-2745", "id_number": "CaltechAUTHORS:20130711-104255460", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130711-104255460", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pew Latin American Fellowship in the Biomedical Sciences" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "T2-00006" }, { "agency": "NIH", "grant_number": "AA020514" }, { "agency": "NIH", "grant_number": "P30DK026743" }, { "agency": "Cincinnati Children's Hospital Research Foundation" }, { "agency": "NIH", "grant_number": "P50 HG004071" }, { "agency": "NIH", "grant_number": "R01DK060322" }, { "agency": "David and Lucile Packard Foundation" } ] }, "doi": "10.1242/dev.097618", "pmcid": "PMC3678343", "primary_object": { "basename": "2734.full.pdf", "url": "https://authors.library.caltech.edu/records/9v261-nfb80/files/2734.full.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Hochgreb-H\u00e4gele, Tatiana; Yin, Chunyue; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fcct6-nnb90", "eprint_id": 40957, "eprint_status": "archive", "datestamp": "2023-09-26 23:51:17", "lastmod": "2023-10-24 14:54:53", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Insights into neural crest development and evolution from genomic analysis", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Published by Cold Spring Harbor Laboratory Press. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. \n\nWe would like to thank Tatiana Hochgreb, Sujata Bhattacharyya,\nand Stephen Green for critical reading of the manuscript and\nhelpful suggestions. This work was supported by NIH grants\nHD037105 and DE16459 (M.E.B.), the Pew Fellows Program in\nthe Biomedical Sciences (M.S-C.), and a Caltech Cell Center fellowship (M.S-C.)\n\nPublished - Genome_Res-2013-Simoes-Costa-1069-80.pdf
", "abstract": "The neural crest is an excellent model system for the study of cell type diversification during embryonic development due\nto its multipotency, motility, and ability to form a broad array of derivatives ranging from neurons and glia, to cartilage,\nbone, and melanocytes. As a uniquely vertebrate cell population, it also offers important clues regarding vertebrate\norigins. In the past 30 yr, introduction of recombinant DNA technology has facilitated the dissection of the genetic\nprogram controlling neural crest development and has provided important insights into gene regulatory mechanisms\nunderlying cell migration and differentiation. More recently, new genomic approaches have provided a platform and\ntools that are changing the depth and breadth of our understanding of neural crest development at a \"systems\" level. Such\nadvances provide an insightful view of the regulatory landscape of neural crest cells and offer a new perspective on\ndevelopmental as well as stem cell and cancer biology.", "date": "2013-07", "date_type": "published", "publication": "Genome Research", "volume": "23", "number": "7", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "1069-1080", "id_number": "CaltechAUTHORS:20130827-110646016", "issn": "1088-9051", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130827-110646016", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Pew Fellows Program in the Biomedical Sciences" }, { "agency": "Caltech Cell Center fellowship" } ] }, "doi": "10.1101/gr.157586.113", "pmcid": "PMC3698500", "primary_object": { "basename": "Genome_Res-2013-Simoes-Costa-1069-80.pdf", "url": "https://authors.library.caltech.edu/records/fcct6-nnb90/files/Genome_Res-2013-Simoes-Costa-1069-80.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Sim\u00f5es-Costa, Marcos and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5ska2-79876", "eprint_id": 39502, "eprint_status": "archive", "datestamp": "2023-08-19 20:08:38", "lastmod": "2023-10-24 16:52:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Vieceli-F-M", "name": { "family": "Vieceli", "given": "Felipe Monteleone" }, "orcid": "0000-0001-5142-8224" }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Turri-J-A", "name": { "family": "Turri", "given": "Jos\u00e9 Antonio" } }, { "id": "Kanno-T", "name": { "family": "Kanno", "given": "Tatiane" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Yan-Chao-Yun-Irene", "name": { "family": "Yan", "given": "Chao Yun Irene" }, "orcid": "0000-0003-0527-5719" } ] }, "title": "The transcription factor chicken Scratch2 is expressed in a subset of early postmitotic neural progenitors", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Elsevier B.V. \n\nReceived 13 March 2012; Received in revised form 19 March 2013; Accepted 21 March 2013; Available online 6 April 2013. \n\nWe thank Dr. Shankar Srinivas for sharing the protocol for in situ hybridization in paraffin sections and Dr. Crist\u00f3v\u00e3o de Albuquerque for critically reading this manuscript. This work was supported by a grant from FAPESP (2009/50544-1) and CNPq to CYIY. FMV was supported by a fellowship from FAPESP.\n\nSupplemental Material - Fig1.png
Supplemental Material - Fig2.png
", "abstract": "Scratch proteins are members of the Snail superfamily which have been shown to regulate invertebrate neural development. However, in vertebrates, little is known about the function of Scratch or its relationship to other neural transcription factors. We report the cloning of chicken Scratch2 (cScrt2) and describe its expression pattern in the chick embryo from HH15 through HH29. cScrt2 was detected in cranial ganglia, the nasal placode and neural tube. At all stages examined, cScrt2 expression is only detected within a subregion of the intermediate zone of the neural tube. cScrt2 is also expressed in the developing dorsal root ganglia from HH22\u201323 onwards and becomes limited to its dorsal medial domain at HH29. phospho-Histone H3 and BrdU-labeling revealed that the cScrt2 expression domain is located immediately external to the proliferative region. In contrast, cScrt2 domain overlapped almost completely with that of the postmitotic neural transcription factor NeuroM/Ath3/NEUROD4. Together, these data define cScrt2-positive cells as a subset of immediately postmitotic neural progenitors. Previous data has shown that Scrt2 is a repressor of E-box-driven transcription whereas NeuroM is an E-box-transactivator. In light of these data, the co-localization detected here suggests that Scrt2 and NeuroM may have opposing roles during definition of neural subtypes.", "date": "2013-06", "date_type": "published", "publication": "Gene Expression Patterns", "volume": "13", "number": "5-6", "publisher": "Elsevier", "pagerange": "189-196", "id_number": "CaltechAUTHORS:20130722-140013924", "issn": "1567-133X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130722-140013924", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "2009/50544-1" }, { "agency": "Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)" } ] }, "doi": "10.1016/j.gep.2013.03.004", "primary_object": { "basename": "Fig1.png", "url": "https://authors.library.caltech.edu/records/5ska2-79876/files/Fig1.png" }, "related_objects": [ { "basename": "Fig2.png", "url": "https://authors.library.caltech.edu/records/5ska2-79876/files/Fig2.png" } ], "resource_type": "article", "pub_year": "2013", "author_list": "Vieceli, Felipe Monteleone; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/k40k8-wxp17", "eprint_id": 39318, "eprint_status": "archive", "datestamp": "2023-08-19 19:17:44", "lastmod": "2023-10-24 16:40:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McKinney-M-C", "name": { "family": "McKinney", "given": "Mary Cathleen" } }, { "id": "Fukatsu-Kazumi", "name": { "family": "Fukatsu", "given": "Kazumi" } }, { "id": "Morrison-J", "name": { "family": "Morrison", "given": "Jason" } }, { "id": "McLennan-R", "name": { "family": "McLennan", "given": "Rebecca" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Kulesa-P-M", "name": { "family": "Kulesa", "given": "Paul M." } } ] }, "title": "Evidence for dynamic rearrangements but lack of fate or position", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2013 FASEB. \n\nGrant Funding Source: NIH grant 1RO1HD057922.", "abstract": "Neural crest cells emerge from the dorsal trunk neural tube and migrate ventrally\nto colonize neuronal derivatives, as well as dorsolaterally to form melanocytes.\nHere, we test whether different dorsoventral levels in the neural tube have similar\nor differential ability to contribute to neural crest cells and their derivatives. To\nthis end, we precisely labeled neural tube precursors at specific dorsoventral levels\nof the chick neural tube using fluorescent dyes and a photoconvertible fluorescent\nprotein. Neural tube and neural crest cell dynamics were then examined in vivo\nand in slice culture using 2-photon and confocal time-lapse imaging. The results\nshow that neural crest precursors undergo dynamic rearrangements within the\nneuroepithelium, yielding an overall ventral to dorsal movement toward the\nmidline of the neural tube, where they exit in a stochastic manner to populate\nmultiple derivatives. No differences were noted in the ability of precursors from\ndifferent dorsoventral levels of the neural tube to contribute to neural crest\nderivatives, with the exception of sympathetic ganglia, which appeared to be\n\"filled\" by the first population to emigrate. Rather than restricted developmental\npotential, however, this is likely due to a matter of timing.", "date": "2013-04", "date_type": "published", "publication": "FASEB Journal", "volume": "27", "publisher": "Federation of American Societies for Experimental Biology", "pagerange": "Art. No. 965.1", "id_number": "CaltechAUTHORS:20130711-105030561", "issn": "0892-6638", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130711-105030561", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "1RO1HD057922" } ] }, "resource_type": "article", "pub_year": "2013", "author_list": "McKinney, Mary Cathleen; Fukatsu, Kazumi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zcsnq-grz32", "eprint_id": 38285, "eprint_status": "archive", "datestamp": "2023-08-19 19:13:20", "lastmod": "2023-10-23 20:00:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Smith-J-J", "name": { "family": "Smith", "given": "Jeramiah J." }, "orcid": "0000-0001-5333-5531" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Jones-M", "name": { "family": "Jones", "given": "Matthew" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Sequencing of the sea lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Nature Publishing Group. \n\nThis work is licensed under a Creative Commons Attribution-\nNonCommercial-Share Alike 3.0 Unported License. To view a copy\nof this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. \n\nReceived 20 July 2012; accepted 31 January 2013; published online 24 February 2013. \n\nWe thank the Genome Institute, Washington University School of Medicine, Production Sequencing group for all sample procurement and genome sequencing work, the Michigan State University Genomic Core for transcriptome sequencing and the US Geological Survey, Lake Huron Biological Station for providing lamprey samples for sequencing. We thank F. Antonacci and E.E. Eichler\n(University of Washington) for performing FISH and providing access to computational facilities, respectively. We thank M. Robinson for bioinformatic analysis of immune system\u2013related genes and conversion of GFF files for BAC end mapping. A portion of this research was conducted at the Marine Biological Laboratory (Woods Hole, Massachusetts). We acknowledge the support of the Stowers Institute for Medical Research (SIMR) and technical support from the SIMR Molecular Biology Core, particularly K. Staehling, A. Perera and\nK. Delventhal for BAC screening and sequencing. We acknowledge the Center for High-Performance Computing at the University of Utah for the allocation of computational resources toward gene annotation. We recognize all the important work that could not be cited owing to space limitations. The lamprey genome project was funded by the National Human Genome Research Institute (U54HG003079 (R.K.W.)). Additional support was provided by grants from the US National Institutes of Health (R24GM83982 (W.L.)) and the Great Lakes Fisheries commission (W.L.). Partial funding was provided by several additional sources, including grants from the US National Institutes of Health (F32GM087919 and T32HG00035 (J.J.S.); DE017911 (M.E.B.); R03NS078519 (O.E.B.); R01HG004694 (M.D.Y.); GM079492, GM090049 and RR014085 (C.T.A.); and R37HD032443 (C.J.T.)), the National Science Foundation (MCB-0719558 (C.T.A.); IOS-0849569 (S.A.S.); IBN-0208138 (L. Holland); and IOS-1126998 (M.D.Y.)), the New Hampshire Agricultural Experiment Station (Scientific Contribution Number 2471 (S.A.S.)), the Charles Evans Research Award (O.E.B., J.D.B. and J.R.M.), the Wellcome Trust (WT095908 (P.F.) and WT098051), the Canadian Institutes of Health Research (MOP74667 (J.P.R.)) and the Natural Sciences and Engineering Research Council of Canada (312221 (J.P.R.)).\n\nAuthor Contributions: \nJ.J.S. developed the assembly, coordinated analyses, performed analyses of genome structure and conserved synteny, coordinated the manuscript, and wrote and edited the manuscript. S.K. contributed to analyses of GC content, assembly completeness, vertebrate-specific genes, myelin-related genes and limb development, and to preparation of the manuscript and supplements. C.H. compiled molecular data sets and developed the consortium gene annotations and annotation pipeline. T.S.-S. developed the protocol for the preparation of BACs, identified the sequenced individual, and prepared genomic DNA for sequencing and BAC library construction. N.J. performed computational identification and analysis of transposable elements. M.D.Y. and M.S.C. contributed to the development of the consortium gene annotations and the annotation pipeline. T.M. and A.M. performed analysis of vertebrate-specific gene families, codon usage bias and amino-acid composition, and contributed to the writing of the manuscript. S.D. and M.H. contributed to analysis of codon usage bias and amino-acid composition. O.E.B., J.R.M., J.D.B. and R.S. performed experiments generating neuronal transcriptomes and data, and sequence analysis related to the vertebrate central nervous system. C.S., L.M.W., A.S.G., M.C. and R.K. performed experiments and data analysis related to the identification and annotation of Hox genes, led and prepared by L.M.W. S.A.S., W.A.D. and J.A.H. performed analyses related to the evolution of neuroendocrine genes, led by S.A.S. and prepared by W.A.D. C.T.A., N.R.S., K.M.B., J.P.R., S.D. and M.H. performed analyses related to the evolution of immune system genes, led and prepared by C.T.A., K.M.B., J.P.R. and M.H. N.R. and C.J.T. performed analyses related to the evolution and development of appendages. P.P. performed BLAST analyses of the noncoding portion of the lamprey genome, and G.E. analyzed BLAST output and wrote the corresponding sections. M.R., B.L.A. and S.M.J.S. developed the Ensembl gene set, led and prepared by M.R. M.M., M.P. and J.H. performed GeneTree and CAFE analysis for the study of whole-genome duplications at the stem of the vertebrate lineage and prepared the corresponding sections. T.S.-S., M.J., J.A.L. and D.W.M. developed the protocol for the preparation of cDNA. N.M. and P.G. provided isolated leukocyte RNA. C.T.B. and K.G.N. performed transcriptome assemblies. W.L., Y.-W.C.-D.,\nS.V.L., C.-Y.Y. and D.W.M. contributed to next-generation transcriptome sequencing. Z.P. provided lamprey leukocyte RNA and cDNA samples and libraries, and evaluated the first draft assembly of the genome. B.F. contributed to the development of neurodevelopment-related text. P.J.d.J. and B.Z. generated the BAC library used for genome sequencing and assembly. L.L.F., W.C.W. and S.W.C. contributed to sequencing project management. B.T. coordinated the cDNA sequencing projects. P.F. supervised the Ensembl annotation efforts. M.E.B. contributed to the conception of the sea lamprey genome project and the development of the manuscript. R.K.W. provided supervision of the genome sequencing project. W.L. provided coordination of the consortium and analysis of the assembly, and contributed to the development of the manuscript.\nCOMPETING FINANCIAL INTERESTS\nThe authors declare no competing financial interests.\n\nPublished - ng.2568.pdf
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", "abstract": "Lampreys are representatives of an ancient vertebrate lineage that diverged from our own ~500 million years ago. By virtue of this deeply shared ancestry, the sea lamprey (P. marinus) genome is uniquely poised to provide insight into the ancestry of vertebrate genomes and the underlying principles of vertebrate biology. Here, we present the first lamprey whole-genome sequence and assembly. We note challenges faced owing to its high content of repetitive elements and GC bases, as well as the absence of broad-scale sequence information from closely related species. Analyses of the assembly indicate that two whole-genome duplications likely occurred before the divergence of ancestral lamprey and gnathostome lineages. Moreover, the results help define key evolutionary events within vertebrate lineages, including the origin of myelin-associated proteins and the development of appendages. The lamprey genome provides an important resource for reconstructing vertebrate origins and the evolutionary events that have shaped the genomes of extant organisms.", "date": "2013-04", "date_type": "published", "publication": "Nature Genetics", "volume": "45", "number": "4", "publisher": "Nature Publishing Group", "pagerange": "415-421", "id_number": "CaltechAUTHORS:20130506-091840430", "issn": "1061-4036", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130506-091840430", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Stowers Institute for Medical Research (SIMR)" }, { "agency": "National Human Genome Research Institute", "grant_number": "U54HG003079" }, { "agency": "NIH", "grant_number": "R24GM83982" }, { "agency": "Great Lakes Fisheries commission" }, { "agency": "NIH", "grant_number": "F32GM087919" }, { "agency": "NIH", "grant_number": "T32HG00035" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "R03NS078519" }, { "agency": "NIH", "grant_number": "R01HG004694" }, { "agency": "NIH", "grant_number": "GM079492" }, { "agency": "NIH", "grant_number": "GM090049" }, { "agency": "NIH", "grant_number": "RR014085" }, { "agency": "NIH", "grant_number": "R37HD032443" }, { "agency": "NSF", "grant_number": "MCB-0719558" }, { "agency": "NSF", "grant_number": "IOS-0849569" }, { "agency": "NSF", "grant_number": "IBN-0208138" }, { "agency": "NSF", "grant_number": "IOS-1126998" }, { "agency": "Wellcome Trust", "grant_number": "WT095908" }, { "agency": "Wellcome Trust", "grant_number": "WT098051" }, { "agency": "Canadian Institutes of Health Research", "grant_number": "MOP74667" }, { "agency": "Natural Sciences and Engineering Research Council of Canada (NSERC)", "grant_number": "312221" }, { "agency": "New Hampshire Agricultural Experiment Station" }, { "agency": "Charles Evans Research Award" } ] }, "doi": "10.1038/ng.2568", "pmcid": "PMC3709584", "primary_object": { "basename": "ng.2568.pdf", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568.pdf" }, "related_objects": [ { "basename": "ng.2568_S7.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S7.xlsx" }, { "basename": "ng.2568_S3.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S3.xlsx" }, { "basename": "ng.2568_S4.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S4.xlsx" }, { "basename": "ng.2568_S5.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S5.xlsx" }, { "basename": "ng.2568_S6.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S6.xlsx" }, { "basename": "ng.2568_S8.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S8.xlsx" }, { "basename": "ng.2568_S1.pdf", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S1.pdf" }, { "basename": "ng.2568_S2.xlsx", "url": "https://authors.library.caltech.edu/records/zcsnq-grz32/files/ng.2568_S2.xlsx" } ], "resource_type": "article", "pub_year": "2013", "author_list": "Smith, Jeramiah J.; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7ejm1-v8t24", "eprint_id": 37575, "eprint_status": "archive", "datestamp": "2023-08-19 19:05:25", "lastmod": "2023-10-23 17:48:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Peng-Brian-N", "name": { "family": "Peng", "given": "Brian N." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2013 Saxena et al.\n\nThis article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.\nReceived October 23, 2012.\nAccepted February 8, 2013.\nPublished March 19, 2013.\nWe thank Dr. Bill Dempsey, Dr. Periklis Pantazis, and Dr. Scott Fraser for sharing the PhOTO-N line pre-publication; LeighAnn Fletcher and David Mayorga for zebrafish husbandry assistance; Dr. Robert Kelsh for the Sox10:eGFP line; Dr. Uwe Strahle for the Ngn1:nRFP line; Dr. Yoshihiro Yoshihara, RIKEN BSI, and National Bioresource Project of Japan for the OMP:RFP and TRPC2:Venus lines; Dr. Bruce Appel for zSox10 antibody; Dr. Andres Collazo for laser ablation expertise; Dr. Scott Fraser and Abigail Saxena for feedback on the manuscript.\n\nAuthor contributions:\nAS, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article.\nBNP, Acquisition of data, Analysis and interpretation of data.\nMEB, Conception and design, Drafting or revising the article.\n\nPublished - e00336.full.pdf
", "abstract": "The sense of smell in vertebrates is detected by specialized sensory neurons derived from the peripheral nervous system. Classically, it has been presumed that the olfactory placode forms all olfactory sensory neurons. In contrast, we show that the cranial neural crest is the primary source of microvillous sensory neurons within the olfactory epithelium of zebrafish embryos. Using photoconversion-based fate mapping and live cell tracking coupled with laser ablation, we followed neural crest precursors as they migrated from the neural tube to the nasal cavity. A subset that coexpressed Sox10 protein and a neurogenin1 reporter ingressed into the olfactory epithelium and differentiated into microvillous sensory neurons. Timed loss-of-function analysis revealed a critical role for Sox10 in microvillous neurogenesis. Taken together, these findings directly demonstrate a heretofore unknown contribution of the cranial neural crest to olfactory sensory neurons in zebrafish and provide important insights into the assembly of the nascent olfactory system.", "date": "2013-03-19", "date_type": "published", "publication": "eLife", "volume": "2", "publisher": "eLife Sciences Publications", "pagerange": "Art. No. e00336", "id_number": "CaltechAUTHORS:20130320-112931982", "issn": "2050-084X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130320-112931982", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE16459" }, { "agency": "NIH", "grant_number": "HG004071" }, { "agency": "Gordon Ross Postdoctoral Fellowship" }, { "agency": "NIH", "grant_number": "5T32NS007251" } ] }, "doi": "10.7554/eLife.00336.001", "pmcid": "PMC3601810", "primary_object": { "basename": "e00336.full.pdf", "url": "https://authors.library.caltech.edu/records/7ejm1-v8t24/files/e00336.full.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Saxena, Ankur; Peng, Brian N.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1rcj7-2e966", "eprint_id": 37588, "eprint_status": "archive", "datestamp": "2023-08-22 08:42:17", "lastmod": "2023-10-23 17:49:41", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rogers-C-D", "name": { "family": "Rogers", "given": "Crystal D." }, "orcid": "0000-0002-9549-1089" }, { "id": "Phillips-J-L", "name": { "family": "Phillips", "given": "Jacquelyn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Elk3 is essential for the progression from progenitor to definitive neural crest cell", "ispublished": "pub", "full_text_status": "public", "keywords": "Elk3; Neural crest specification; Progenitors; Pax7", "note": "\u00a9 2012 Elsevier Inc. \n\nReceived 30 August 2012; Received in revised form; 13 December 2012; Accepted 14 December 2012; Available online 22 December 2012. \n\nWe would like to thank Laura Gammill and Meghan Adams for previous identification of Elk3 and the Bronner lab for helpful discussions. This work was supported by NIH grant HD037105 to MEB, NIH minority supplement P01 HD037105 to CDR, and an Amgen summer fellowship and SURF fellowship to JLP.\n\nAccepted Version - nihms431566.pdf
", "abstract": "Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we characterize the early spatiotemporal expression pattern of Elk3 in the avian embryo using whole mount in situ hybridization and quantitative RT-PCR and examine the effects of its loss of function on neural crest development. At early stages, Elk3 is expressed in the head folds, head mesenchyme, intersomitic vessels, and migratory cranial neural crest (NC) cells. Loss of the Elk3 protein results in the retention of Pax7+ precursors in the dorsal neural tube that fail to upregulate neural crest specifier genes, FoxD3, Sox10 and Snail2, resulting in embryos with severe migration defects. The results putatively place Elk3 downstream of neural plate border genes, but upstream of neural crest specifier genes in the neural crest gene regulatory network (NC-GRN), suggesting that it is critical for the progression from progenitor to definitive neural crest cell.", "date": "2013-02-15", "date_type": "published", "publication": "Developmental Biology", "volume": "374", "number": "2", "publisher": "Elsevier", "pagerange": "255-263", "id_number": "CaltechAUTHORS:20130321-131533041", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130321-131533041", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "P01 HD037105" }, { "agency": "Amgen" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" } ] }, "doi": "10.1016/j.ydbio.2012.12.009", "pmcid": "PMC3558539", "primary_object": { "basename": "nihms431566.pdf", "url": "https://authors.library.caltech.edu/records/1rcj7-2e966/files/nihms431566.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Rogers, Crystal D.; Phillips, Jacquelyn L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zan9a-dmb68", "eprint_id": 37248, "eprint_status": "archive", "datestamp": "2023-08-23 16:48:16", "lastmod": "2023-10-23 17:17:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McKinney-M-C", "name": { "family": "McKinney", "given": "Mary C." } }, { "id": "Fukatsu-Kazumi", "name": { "family": "Fukatsu", "given": "Kazumi" } }, { "id": "Morrison-J", "name": { "family": "Morrison", "given": "Jason" } }, { "id": "McLennan-R", "name": { "family": "McLennan", "given": "Rebecca" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Kulesa-P-M", "name": { "family": "Kulesa", "given": "Paul M." } } ] }, "title": "Evidence for dynamic rearrangements but lack of fate or position restrictions in premigratory avian trunk neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Chick, Neural tube, Neural crest, Trunk, Cell fate, Cell tracing, Photoconversion", "note": "\u00a9 2013 Published by The Company of Biologists Ltd. \n\nAccepted November 30, 2012. Posted online before print January 14, 2013. \n\nGene expression profiling samples were analyzed by the Fluidigm Genetic Analysis Facility of the Molecular Genetics Core Facility at Children's Hospital Boston. Gap43-TagRFP was kindly prepared by DRI and H2B-eGFP was a kind gift from Robb Krumlauf. We thank Connie Gonzalez for technical assistance with in situ hybridization. \n\nFunding: P.K. was partially funded by the National Institutes of Health (NIH) [NIH-1R01HD057922] and the Stowers Institute for Medical Research. M.E.B. was partially funded [HD037105 and DE16459]. The Molecular Genetics Core Facility at Children's Hospital Boston is supported by the NIH [NIH-P30-HD18655]. Deposited in PMC for release after 12 months. \n\nCompeting interests statement: The authors declare no competing financial interests.\n\nPublished - 820.full.pdf
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", "abstract": "Neural crest (NC) cells emerge from the dorsal trunk neural tube (NT) and migrate ventrally to colonize neuronal derivatives, as well as dorsolaterally to form melanocytes. Here, we test whether different dorsoventral levels in the NT have similar or differential ability to contribute to NC cells and their derivatives. To this end, we precisely labeled NT precursors at specific dorsoventral levels of the chick NT using fluorescent dyes and a photoconvertible fluorescent protein. NT and NC cell dynamics were then examined in vivo and in slice culture using two-photon and confocal time-lapse imaging. The results show that NC precursors undergo dynamic rearrangements within the neuroepithelium, yielding an overall ventral to dorsal movement toward the midline of the NT, where they exit in a stochastic manner to populate multiple derivatives. No differences were noted in the ability of precursors from different dorsoventral levels of the NT to contribute to NC derivatives, with the exception of sympathetic ganglia, which appeared to be 'filled' by the first population to emigrate. Rather than restricted developmental potential, however, this is probably due to a matter of timing.", "date": "2013-02-15", "date_type": "published", "publication": "Development", "volume": "140", "number": "4", "publisher": "Company of Biologists", "pagerange": "820-830", "id_number": "CaltechAUTHORS:20130301-114905448", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130301-114905448", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NIH-1R01HD057922" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Stowers Institute for Medical Research" }, { "agency": "NIH", "grant_number": "NIH-P30-HD18655" } ] }, "doi": "10.1242/dev.083725", "pmcid": "PMC3557777", "primary_object": { "basename": "Movie4.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie4.mov" }, "related_objects": [ { "basename": "Movie5.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie5.mov" }, { "basename": "Movie7.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie7.mov" }, { "basename": "Movie2.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie2.mov" }, { "basename": "DEV083725.pdf", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/DEV083725.pdf" }, { "basename": "Movie1.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie1.mov" }, { "basename": "Movie10.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie10.mov" }, { "basename": "Movie3.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie3.mov" }, { "basename": "Movie6.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie6.mov" }, { "basename": "Movie8.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie8.mov" }, { "basename": "Movie9.mov", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/Movie9.mov" }, { "basename": "820.full.pdf", "url": "https://authors.library.caltech.edu/records/zan9a-dmb68/files/820.full.pdf" } ], "resource_type": "article", "pub_year": "2013", "author_list": "McKinney, Mary C.; Fukatsu, Kazumi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/adeq5-g3566", "eprint_id": 36296, "eprint_status": "archive", "datestamp": "2023-08-22 08:38:12", "lastmod": "2023-10-20 22:59:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hochgreb-H\u00e4gele-T", "name": { "family": "Hochgreb-H\u00e4gele", "given": "Tatiana" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A novel FoxD3 gene trap line reveals neural crest precursor movement and a role for FoxD3 in their specification", "ispublished": "pub", "full_text_status": "public", "keywords": "FoxD3; Neural crest; Zebrafish; Live imaging; Mutant", "note": "\u00a9 2012 Elsevier Inc. \n\nAvailable online 8 December 2012. \n\nWe thank L. Trinh, T. Sauka-Spengler and M. Sim\u00f5es-Costa for comments on the manuscript and helpful discussions, Tom Schilling for sharing foxd3 probe and Tg(FoxD3:EGFP) line, Leigh Ann Fletcher for fish care, Ilana Solomon, Neha Das and Joanne Tan for technical support and Daniel Koo for help with vector analysis. T.H.-H. was supported by a Pew Latin American Fellowship in Biomedical Sciences and by California Institute for Regenerative Medicine Training Grant (T2-00006). This work was supported by NIH grants HG004071 and HD037105 (M.E.B).\n\nAccepted Version - nihms585091.pdf
", "abstract": "Neural crest cells migrate extensively and contribute to diverse derivatives, including the craniofacial skeleton, peripheral neurons and glia, and pigment cells. Although several transgenic lines label neural crest subpopulations, few are suited for studying early events in neural crest development. Here, we present a zebrafish gene/protein trap line gt(foxd3-citrine)^(ct110a) that expresses a Citrine fusion protein with FoxD3, a transcription factor expressed in premigratory and migrating neural crest cells. In this novel line, citrine expression exactly parallels endogenous foxd3 expression. High-resolution time-lapse imaging reveals the dynamic phases of precursor and migratory neural crest cell movements from the neural keel stage to times of active cell migration. In addition, Cre-recombination produces a variant line FoxD3-mCherry-pA whose homozygosis generates a FoxD3 mutant. Taking advantage of the endogenously regulated expression of FoxD3-mCherry fusion protein, we directly assess early effects of FoxD3 loss-of-function on specification and morphogenesis of dorsal root ganglia, craniofacial skeleton and melanophores. These novel lines provide new insights and useful new tools for studying specification, migration and differentiation of neural crest cells.", "date": "2013-02-01", "date_type": "published", "publication": "Developmental Biology", "volume": "374", "number": "1", "publisher": "Elsevier", "pagerange": "1-11", "id_number": "CaltechAUTHORS:20130110-101011580", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130110-101011580", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Pew Latin American Fellowship in Biomedical Sciences" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "T2-00006" }, { "agency": "NIH", "grant_number": "HG004071" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1016/j.ydbio.2012.11.035", "pmcid": "PMC3553214", "primary_object": { "basename": "nihms585091.pdf", "url": "https://authors.library.caltech.edu/records/adeq5-g3566/files/nihms585091.pdf" }, "resource_type": "article", "pub_year": "2013", "author_list": "Hochgreb-H\u00e4gele, Tatiana and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/x2z1g-jyk13", "eprint_id": 37663, "eprint_status": "archive", "datestamp": "2023-08-22 08:33:09", "lastmod": "2023-10-23 17:55:17", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Green-S-A", "name": { "family": "Green", "given": "Stephen A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Gene duplications and the early evolution of neural crest development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; Evolution; Gene duplication", "note": "\u00a9 2013 Elsevier Ltd. \n\nAvailable online 31 December 2012. \n\nWe would like to thank the members of the Bronner Laboratory for their helpful comments. This work was supported by DE017911 and GM090049.", "abstract": "Neural crest cells are an important cell type present in all vertebrates, and elaboration of the neural crest is thought to have been a key factor in their evolutionary success. Genomic comparisons suggest there were two major genome duplications in early vertebrate evolution, raising the possibility that evolution of neural crest was facilitated by gene duplications. Here, we review the process of early neural crest formation and its underlying gene regulatory network (GRN) as well as the evolution of important neural crest derivatives. In this context, we assess the likelihood that gene and genome duplications capacitated neural crest evolution, particularly in light of novel data arising from invertebrate chordates.", "date": "2013-02", "date_type": "published", "publication": "Seminars in Cell and Developmental Biology", "volume": "24", "number": "2", "publisher": "Elsevier", "pagerange": "95-100", "id_number": "CaltechAUTHORS:20130328-084449579", "issn": "1084-9521", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130328-084449579", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "GM090049" } ] }, "doi": "10.1016/j.semcdb.2012.12.006", "resource_type": "article", "pub_year": "2013", "author_list": "Green, Stephen A. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/34978-hph94", "eprint_id": 36709, "eprint_status": "archive", "datestamp": "2023-08-19 13:39:23", "lastmod": "2023-10-23 15:24:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos S." }, "orcid": "0000-0003-1452-7068" }, { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja J." } }, { "id": "Tan-Cabugao-J", "name": { "family": "Tan-Cabugao", "given": "Joanne" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dynamic and Differential Regulation of Stem Cell Factor FoxD3 in the Neural Crest Is Encrypted in the Genome", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 Sim\u00f5es-Costa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived March 12, 2012; Accepted October 18, 2012; Published December 20, 2012. \n\nThis work was supported by NIH grant HD037105 to MEB, a NHMRC CJ Martin Fellowship (400433) to SJM, and a Pew Foundation Fellowship to MSS-C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.\n\nWe thank Hisato Kondoh for the ptk-eGFP construct, Mirella Dottori for\nFoxD3 in situ probe, and Patricia Labosky and Michelle Southard-Smith\nfor the anti-FoxD3 antibody. We also thank Michael Stone and Brian Jun\nfor excellent technical assistance.\nAuthor Contributions:\nConceived and designed the experiments: MSS-C SJM TS-S MEB.\nPerformed the experiments: MSS-C SJM JT-C TS-S. Analyzed the data:\nMSS-C SJM TS-S MEB. Wrote the paper: MSS-C SJM TS-S MEB.\n\nPublished - journal.pgen.1003142.pdf
Published - journal.pgen.1003142.s005.docx
Supplemental Material - journal.pgen.1003142.s001.tif
Supplemental Material - journal.pgen.1003142.s002.tif
Supplemental Material - journal.pgen.1003142.s003.tif
Supplemental Material - journal.pgen.1003142.s004.docx
Supplemental Material - journal.pgen.1003142.s006.docx
Supplemental Material - journal.pgen.1003142.s007.m4v
Supplemental Material - journal.pgen.1003142.s008.m4v
", "abstract": "The critical stem cell transcription factor FoxD3 is expressed by the premigratory and migrating neural crest, an embryonic stem cell population that forms diverse derivatives. Despite its important role in development and stem cell biology, little is known about what mediates FoxD3 activity in these cells. We have uncovered two FoxD3 enhancers, NC1 and NC2, that drive reporter expression in spatially and temporally distinct manners. Whereas NC1 activity recapitulates initial FoxD3 expression in the cranial neural crest, NC2 activity recapitulates initial FoxD3 expression at vagal/trunk levels while appearing only later in migrating cranial crest. Detailed mutational analysis, in vivo chromatin immunoprecipitation, and morpholino knock-downs reveal that transcription factors Pax7 and Msx1/2 cooperate with the neural crest specifier gene, Ets1, to bind to the cranial NC1 regulatory element. However, at vagal/trunk levels, they function together with the neural plate border gene, Zic1, which directly binds to the NC2 enhancer. These results reveal dynamic and differential regulation of FoxD3 in distinct neural crest subpopulations, suggesting that heterogeneity is encrypted at the regulatory level. Isolation of neural crest enhancers not only allows establishment of direct regulatory connections underlying neural crest formation, but also provides valuable tools for tissue specific manipulation and investigation of neural crest cell identity in amniotes.", "date": "2012-12", "date_type": "published", "publication": "PLoS Genetics", "volume": "8", "number": "12", "publisher": "Public Library of Science", "pagerange": "Art. No. e1003142", "id_number": "CaltechAUTHORS:20130131-095347879", "issn": "1553-7390", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130131-095347879", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "National Health and Medical Research Council (NHMRC)", "grant_number": "400433" }, { "agency": "Pew Foundation" } ] }, "doi": "10.1371/journal.pgen.1003142", "pmcid": "PMC3527204", "primary_object": { "basename": "journal.pgen.1003142.s002.tif", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s002.tif" }, "related_objects": [ { "basename": "journal.pgen.1003142.s003.tif", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s003.tif" }, { "basename": "journal.pgen.1003142.s005.docx", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s005.docx" }, { "basename": "journal.pgen.1003142.s006.docx", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s006.docx" }, { "basename": "journal.pgen.1003142.s001.tif", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s001.tif" }, { "basename": "journal.pgen.1003142.s004.docx", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s004.docx" }, { "basename": "journal.pgen.1003142.s007.m4v", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s007.m4v" }, { "basename": "journal.pgen.1003142.s008.m4v", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.s008.m4v" }, { "basename": "journal.pgen.1003142.pdf", "url": "https://authors.library.caltech.edu/records/34978-hph94/files/journal.pgen.1003142.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Sim\u00f5es-Costa, Marcos S.; McKeown, Sonja J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/s0602-sq435", "eprint_id": 35429, "eprint_status": "archive", "datestamp": "2023-08-19 13:26:17", "lastmod": "2023-10-20 16:12:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ishii-Mamoru", "name": { "family": "Ishii", "given": "Mamoru" } }, { "id": "Arias-A-C", "name": { "family": "Arias", "given": "Athena C." } }, { "id": "Liu-Liqiong", "name": { "family": "Liu", "given": "Liqiong" } }, { "id": "Chen-Yi-Bu", "name": { "family": "Chen", "given": "Yi-Bu" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Maxson-R-E", "name": { "family": "Maxson", "given": "Robert E." } } ] }, "title": "A Stable Cranial Neural Crest Cell Line from Mouse", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 Mary Ann Liebert, Inc. \n\nReceived March 26, 2012; Accepted after revision August 13, 2012; Prepublished on Liebert Instant Online August 13, 2012; Online Ahead of Print: October 4, 2012. \n\nWe thank Dr. Baruch Frenkel for providing Bmp2, and we thank members of the Maxson lab for helpful discussions. This work was supported by grants from the National Institutes of Health R01DE016320 and R01DE019650 to R.M. and the CIRM training grant TB1-01192 to A.A. \n\nNo competing financial interests exist in this study.\n\nPublished - scd.2012.0155.pdf
Supplemental Material - Supp_Data1.pdf
Supplemental Material - Supp_Data2.pdf
Supplemental Material - Supp_Data3.pdf
", "abstract": "Cranial neural crest cells give rise to ectomesenchymal derivatives such as cranial bones, cartilage, smooth\nmuscle, dentin, as well as melanocytes, corneal endothelial cells, and neurons and glial cells of the peripheral\nnervous system. Previous studies have suggested that although multipotent stem-like cells may exist during the\ncourse of cranial neural crest development, they are transient, undergoing lineage restriction early in embryonic\ndevelopment. We have developed culture conditions that allow cranial neural crest cells to be grown as multipotent\nstem-like cells. With these methods, we obtained 2 independent cell lines, O9-1 and i10-1, which were\nderived from mass cultures of Wnt1-Cre; R26R-GFP-expressing cells. These cell lines can be propagated and\npassaged indefinitely, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells, and glial cells.\nWhole-genome expression profiling of O9-1 cells revealed that this line stably expresses stem cell markers (CD44,\nSca-1, and Bmi1) and neural crest markers (AP-2\u03b1, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1).\nO9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates when injected into E13.5 mouse cranial tissue explants and chicken embryos. These results suggest that O9-1 cells represent multipotent mesenchymal cranial neural crest cells. The O9-1 cell line should serve as a useful tool for investigating the molecular properties of differentiating cranial neural crest cells.", "date": "2012-11-07", "date_type": "published", "publication": "Stem Cells and Development", "volume": "21", "number": "17", "publisher": "Mary Ann Liebert", "pagerange": "3069-3080", "id_number": "CaltechAUTHORS:20121113-113327641", "issn": "1547-3287", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121113-113327641", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE016320" }, { "agency": "NIH", "grant_number": "R01DE019650" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "TB1-01192" } ] }, "doi": "10.1089/scd.2012.0155", "pmcid": "PMC3495126", "primary_object": { "basename": "scd.2012.0155.pdf", "url": "https://authors.library.caltech.edu/records/s0602-sq435/files/scd.2012.0155.pdf" }, "related_objects": [ { "basename": "Supp_Data1.pdf", "url": "https://authors.library.caltech.edu/records/s0602-sq435/files/Supp_Data1.pdf" }, { "basename": "Supp_Data2.pdf", "url": "https://authors.library.caltech.edu/records/s0602-sq435/files/Supp_Data2.pdf" }, { "basename": "Supp_Data3.pdf", "url": "https://authors.library.caltech.edu/records/s0602-sq435/files/Supp_Data3.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Ishii, Mamoru; Arias, Athena C.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pbm8m-yw712", "eprint_id": 36257, "eprint_status": "archive", "datestamp": "2023-08-19 13:24:37", "lastmod": "2023-10-20 22:56:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A career at the interface of cell and developmental biology: a view from the crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 Bronner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).\nI thank my family for their constant support and in particular my children, Paige and Ryan, for teaching me to be organized and making me realize that running my lab is just like running a family. I thank Scott Fraser for his important influence in the early stages of my career. I thank my wonderful postdocs and students, who have made my career so fulfilling. My greatest professional joy comes from watching them succeed and push forward the frontiers of science. Finally, I thank Carole LaBonne, Laura Gammill, and Marcos Simoes-Costa for helpful comments on this essay and the National Institutes of Health for 30+ years of funding.\n\nPublished - Bronner2012p19503Mol_Biol_Cell.pdf
", "abstract": "Just as neural crest cells migrate great distances through the embryo, my journey has taken me from a childhood in a distant land to a career as a biologist. My mentoring relationships have shaped not only the careers of my trainees, but also the trajectory of my own science. One of the most satisfying aspects of mentoring comes from helping to empower the next generation of scientists to do more tomorrow than is possible today. This, together with a passion for discovery and learning new things, motivates me and makes science such a rewarding career.", "date": "2012-11-01", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "23", "number": "21", "publisher": "American Society for Cell Biology", "pagerange": "4151-4153", "id_number": "CaltechAUTHORS:20130109-083747783", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130109-083747783", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" } ] }, "doi": "10.1091/mbc.E12-05-0389", "pmcid": "PMC3484089", "primary_object": { "basename": "Bronner2012p19503Mol_Biol_Cell.pdf", "url": "https://authors.library.caltech.edu/records/pbm8m-yw712/files/Bronner2012p19503Mol_Biol_Cell.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rxgrw-7rx35", "eprint_id": 35267, "eprint_status": "archive", "datestamp": "2023-08-22 07:41:05", "lastmod": "2023-10-20 15:54:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hu-Na", "name": { "family": "Hu", "given": "Na" } }, { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo" }, "orcid": "0000-0003-0591-6168" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "DNA methyltransferase3A as a molecular switch mediating the neural tube-to-neural crest fate transition", "ispublished": "pub", "full_text_status": "public", "keywords": "DNMT3A; neural tube; neural crest; Sox2; Sox3", "note": "\u00a9 2012 by Cold Spring Harbor Laboratory Press.\nReceived June 17, 2012; revised version accepted September\n13, 2012.\nWe thank Drs. M. Simoes-Costa and M. Barembaum for helpful discussions.\nWe are grateful to Dr. Yoshio Wakamatsu for kindly providing the\nSox2 overexpression construct. This work was supported by F31DE021643\nand 5 T32 GM07616 to N.H., and HD037105 and DE16459 to M.E.B.\n\nPublished - Genes_Dev.-2012-Hu-2380-5.pdf
Supplemental Material - Supplemental_Info.pdf
", "abstract": "Here, we explore whether silencing via promoter DNA methylation plays a role in neural versus neural crest cell lineage decisions. We show that DNA methyltransferase3A (DNMT3A) promotes neural crest specification by directly mediating repression of neural genes like Sox2 and Sox3. DNMT3A is expressed in the neural plate border, and its knockdown causes ectopic Sox2 and Sox3 expression at the expense of neural crest markers. In vivo chromatin immunoprecipitation of neural folds demonstrates that DNMT3A specifically associates with CpG islands in the Sox2 and Sox3 promoter regions, resulting in their repression by methylation. Thus, DNMT3A functions as a molecular switch, repressing neural to favor neural crest cell fate.", "date": "2012-11-01", "date_type": "published", "publication": "Genes and Development", "volume": "26", "number": "21", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "2380-2385", "id_number": "CaltechAUTHORS:20121102-112018740", "issn": "0890-9369", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121102-112018740", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "F31DE021643" }, { "agency": "NIH", "grant_number": "5 T32 GM07616" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1101/gad.198747.112", "pmcid": "PMC3489996", "primary_object": { "basename": "Genes_Dev.-2012-Hu-2380-5.pdf", "url": "https://authors.library.caltech.edu/records/rxgrw-7rx35/files/Genes_Dev.-2012-Hu-2380-5.pdf" }, "related_objects": [ { "basename": "Supplemental_Info.pdf", "url": "https://authors.library.caltech.edu/records/rxgrw-7rx35/files/Supplemental_Info.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Hu, Na; Strobl-Mazzulla, Pablo; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/m9nn1-h7h72", "eprint_id": 35850, "eprint_status": "archive", "datestamp": "2023-08-19 13:15:24", "lastmod": "2023-10-20 21:51:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Jayasena-C-S", "name": { "family": "Jayasena", "given": "Chathurani S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-\u03b2 signaling", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 by The Rockefeller University Press. \nThis article is distributed under the terms of an Attribution\u2013\nNoncommercial\u2013Share Alike\u2013No Mirror Sites license for the first six months after the publication\ndate (see http://www.rupress.org/terms). After six months it is available under a\nCreative Commons License (Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported license,\nas described at http://creativecommons.org/licenses/by-nc-sa/3.0/). \n\nSubmitted: 26 April 2012; accepted: 25 September 2012. \n\nWe wish to thank the following people: Dr. Le. A. Trinh and Bronner laboratory members for helpful discussions and technical help, Ho-yin Leung for performing 3' RACE, and Leigh Ann Fletcher and David Mayorga from the Caltech Centers of Excellence in Genomic Science fish facility for\nfish maintenance. This work was supported by the US Public Health Service Corps grants P50HG004071, DE017911, and HD037105 to M.E. Bronner. Author contributions: C.S. Jayasena designed and performed the experiments, executed the data analysis, and wrote and edited the manuscript. M. Bronner performed data analysis and edited the manuscript.\n\nPublished - J_Cell_Biol-2012-Jayasena-453-66.pdf
Supplemental Material - JCB_201204138_sm.pdf
", "abstract": "Cranial neural crest cells form much of the facial skeleton, and abnormalities in their development lead to severe birth defects. In a novel zebrafish protein trap screen, we identified an RNA-binding protein, Rbms3, that is transiently expressed in the cytoplasm of condensing neural crest cells within the pharyngeal arches. Morphants for rbms3 displayed reduced proliferation of prechondrogenic crest and significantly altered expression for chondrogenic/osteogenic lineage markers. This phenotype strongly resembles cartilage/crest defects observed in Tgf-\u03b2r2:Wnt1-Cre mutants, which suggests a possible link with TGF-\u03b2 signaling. Consistent with this are the findings that: (a) Rbms3 stabilized a reporter transcript with smad2 3\u2032 untranslated region, (b) RNA immunoprecipitation with full-length Rbms3 showed enrichment for smad2/3, and (c) pSmad2 levels were reduced in rbms3 morphants. Overall, these results suggest that Rbms3 posttranscriptionally regulates one of the major pathways that promotes chondrogenesis, the transforming growth factor \u03b2 receptor (TGF-\u03b2r) pathway.", "date": "2012-10-29", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "199", "number": "3", "publisher": "Rockefeller University Press", "pagerange": "453-466", "id_number": "CaltechAUTHORS:20121206-101410189", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121206-101410189", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P50HG004071" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1083/jcb.201204138", "pmcid": "PMC3483135", "primary_object": { "basename": "JCB_201204138_sm.pdf", "url": "https://authors.library.caltech.edu/records/m9nn1-h7h72/files/JCB_201204138_sm.pdf" }, "related_objects": [ { "basename": "J_Cell_Biol-2012-Jayasena-453-66.pdf", "url": "https://authors.library.caltech.edu/records/m9nn1-h7h72/files/J_Cell_Biol-2012-Jayasena-453-66.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Jayasena, Chathurani S. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rm7vk-hn057", "eprint_id": 35491, "eprint_status": "archive", "datestamp": "2023-08-22 07:01:27", "lastmod": "2023-10-20 16:24:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Epithelial to mesenchymal transition: New and old insights from the classical neural crest model", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; EMT; Development", "note": "\u00a9 2012 Elsevier Ltd. \n\nSome of the work reviewed here is based on studies supported by the National Institutes of Health under Award Nos. R03DE022521 to MEB and PS-M, HD037105 and The California Institute of Technology.\n\nAccepted Version - nihms374229.pdf
", "abstract": "The epithelial-to-mesenchymal transition (EMT) is an important event converting compact and ordered epithelial cells into migratory mesenchymal cells. Given the molecular and cellular similarities between pathological and developmental EMTs, studying this event during neural crest development offers and excellent in vivo model for understanding the mechanisms underlying this process. Here, we review new and old insight into neural crest EMT in search of commonalities with cancer progression that might aid in the design of specific therapeutic prevention.", "date": "2012-10", "date_type": "published", "publication": "Seminars in Cancer Biology", "volume": "22", "number": "5-6", "publisher": "Elsevier", "pagerange": "411-416", "id_number": "CaltechAUTHORS:20121115-133132015", "issn": "1044-579X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121115-133132015", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R03DE022521" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Caltech" } ] }, "doi": "10.1016/j.semcancer.2012.04.008", "pmcid": "PMC3435443", "primary_object": { "basename": "nihms374229.pdf", "url": "https://authors.library.caltech.edu/records/rm7vk-hn057/files/nihms374229.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Strobl-Mazzulla, Pablo H. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/75xbf-kzq26", "eprint_id": 35222, "eprint_status": "archive", "datestamp": "2023-08-19 12:47:07", "lastmod": "2023-10-20 15:51:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo H." }, "orcid": "0000-0003-0591-6168" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A PHD12\u2013Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 Strobl-Mazzulla and Bronner. This article is distributed under the terms of an Attribution\u2013Noncommercial\u2013Share Alike\u2013No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). \n\nSubmitted: 19 March 2012. Accepted: 21 August 2012. \n\nWe are indebted to Dr. Tatjana Sauka-Spengler for her help and advice throughout this project and for the final design of the BiFC vectors to work in chick cells. We are grateful to Dr. Chang-Deng Hu and Dr. Patricia Labosky for kindly providing invaluable reagents of the BiFC vectors and FoxD3 antibody, respectively, as well as Joanna Tan-Cabuga for excellent technical assistance. This work was supported by DE16459 and HD037105 grants to M.E. Bronner and a Fogarty grant R03 DE022521 to M.E. Bronner and P.H. Strobl-Mazzulla.\n\nPublished - 999.full.pdf
Supplemental Material - JCB_201203098_sm.pdf
Supplemental Material - J_Cell_Biol-2012-Strobl-Mazzulla-999-1010.pdf
", "abstract": "Neural crest cells form within the neural tube and then undergo an epithelial to mesenchymal transition (EMT) to initiate migration to distant locations. The transcriptional repressor Snail2 has been implicated in neural crest EMT via an as of yet unknown mechanism. We report that the adaptor protein PHD12 is highly expressed before neural crest EMT. At cranial levels, loss of PHD12 phenocopies Snail2 knockdown, preventing transcriptional shutdown of the adhesion molecule Cad6b (Cadherin6b), thereby inhibiting neural crest emigration. Although not directly binding to each other, PHD12 and Snail2 both directly interact with Sin3A in vivo, which in turn complexes with histone deacetylase (HDAC). Chromatin immunoprecipitation revealed that PHD12 is recruited to the Cad6b promoter during neural crest EMT. Consistent with this, lysines on histone 3 at the Cad6b promoter are hyperacetylated before neural crest emigration, correlating with active transcription, but deacetylated during EMT, reflecting the repressive state. Knockdown of either PHD12 or Snail2 prevents Cad6b promoter deacetylation. Collectively, the results show that PHD12 interacts directly with Sin3A/HDAC, which in turn interacts with Snail2, forming a complex at the Cad6b promoter and thus revealing the nature of the in vivo Snail repressive complex that regulates neural crest EMT.", "date": "2012-09-17", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "198", "number": "6", "publisher": "Rockefeller University Press", "pagerange": "999-1010", "id_number": "CaltechAUTHORS:20121101-081935033", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121101-081935033", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "R03 DE022521" } ] }, "doi": "10.1083/jcb.201203098", "pmcid": "PMC3444776", "primary_object": { "basename": "999.full.pdf", "url": "https://authors.library.caltech.edu/records/75xbf-kzq26/files/999.full.pdf" }, "related_objects": [ { "basename": "JCB_201203098_sm.pdf", "url": "https://authors.library.caltech.edu/records/75xbf-kzq26/files/JCB_201203098_sm.pdf" }, { "basename": "J_Cell_Biol-2012-Strobl-Mazzulla-999-1010.pdf", "url": "https://authors.library.caltech.edu/records/75xbf-kzq26/files/J_Cell_Biol-2012-Strobl-Mazzulla-999-1010.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Strobl-Mazzulla, Pablo H. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ajcff-wv957", "eprint_id": 35378, "eprint_status": "archive", "datestamp": "2023-08-22 06:27:43", "lastmod": "2023-10-20 16:09:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Fishwick-K-J", "name": { "family": "Fishwick", "given": "Katherine J." } }, { "id": "Neiderer-T-E", "name": { "family": "Neiderer", "given": "Theresa E." } }, { "id": "Jhingory-S", "name": { "family": "Jhingory", "given": "Sharon" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Taneyhill-L-A", "name": { "family": "Taneyhill", "given": "Lisa A." } } ] }, "title": "The tight junction protein claudin-1 influences cranial neural crest cell emigration", "ispublished": "pub", "full_text_status": "public", "keywords": "Claudin-1; Tight junctions; Neural crest; Placodes; EMT", "note": "\u00a9 2012 Elsevier Ireland Ltd. \n\nReceived 3 April 2012; Received in revised form 5 June 2012; Accepted 26 June 2012; Available online 3 July 2012. \n\nThe authors would like to thank Ms. Abigail Figat for technical assistance. This work was supported by Grants NIH-HD037105 and DE16459 (M.E.B.) and NSF IOS-0948525 (L.A.T.). Additional support for this research was provided by the University of Maryland from the Howard Hughes Medical Institute Undergraduate Science Education Program (T.E.N.).\n\nAccepted Version - nihms391306.pdf
", "abstract": "The neural crest is a population of migratory cells that follows specific pathways during development, eventually differentiating to form parts of the face, heart, and peripheral nervous system, the latter of which includes contributions from placodal cells derived from the ectoderm. Stationary, premigratory neural crest cells acquire the capacity to migrate by undergoing an epithelial-to-mesenchymal transition that facilitates their emigration from the dorsal neural tube. This emigration involves, in part, the dismantling of cell-cell junctions, including apically localized tight junctions in the neuroepithelium. In this study, we have characterized the role of the transmembrane tight junction protein claudin-1 during neural crest and placode ontogeny. Our data indicate that claudin-1 is highly expressed in the developing neuroepithelium but is down-regulated in migratory neural crest cells, although expression persists in the ectoderm from which the placode cells arise. Depletion or overexpression of claudin-1 augments or reduces neural crest cell emigration, respectively, but does not impact the development of several cranial placodes. Taken together, our results reveal a novel function for a tight junction protein in the formation of migratory cranial neural crest cells in the developing vertebrate embryo.", "date": "2012-09", "date_type": "published", "publication": "Mechanisms of Development", "volume": "129", "number": "9-12", "publisher": "Elsevier", "pagerange": "275-283", "id_number": "CaltechAUTHORS:20121109-094813120", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121109-094813120", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "NSF", "grant_number": "IOS-0948525" }, { "agency": "University of Maryland" }, { "agency": "Howard Hughes Medical Institute (HHMI)" } ] }, "doi": "10.1016/j.mod.2012.06.006", "pmcid": "PMC3482127", "primary_object": { "basename": "nihms391306.pdf", "url": "https://authors.library.caltech.edu/records/ajcff-wv957/files/nihms391306.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Fishwick, Katherine J.; Neiderer, Theresa E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8nzhy-pz130", "eprint_id": 34537, "eprint_status": "archive", "datestamp": "2023-08-22 06:18:18", "lastmod": "2023-10-19 20:43:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Formation and migration of neural crest cells in the vertebrate embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Neural tube; EMT; Transcription factor; Chromatin; Cis-regulatory network", "note": "\u00a9 2012 Springer-Verlag. \n\nAccepted: 20 June 2012; Published online: 22 July 2012. \n\nRobert Feulgen Lecture presented at the 54th Symposium of the Society for Histochemistry in Vienna, Austria, 5\u20138 September 2012. \n\nThis work was partially supported by NIH Grant HD037105 to MEB. I thank Drs. Marcos Simoes-Costa, Shuyi Nie, and Pablo Strobl-Mazzulla for providing figures.\n\nAccepted Version - nihms395640.pdf
", "abstract": "The neural crest is a stem cell population,\nunique to vertebrates, that gives rise to a vast array of\nderivatives, ranging from peripheral ganglia to the facial\nskeleton. This population is induced in the early embryo at\nthe border of the neural plate, which will form the central\nnervous system (CNS). After neural tube closure, neural\ncrest cells depart from the dorsal CNS via an epithelial to\nmesenchymal transition (EMT), forming a migratory\nmesenchymal cell type that migrates extensive to diverse\nlocations in the embryo. Using in vivo loss-of-function\napproaches and cis-regulatory analysis coupled with live\nimaging, we have investigated the gene regulatory network\nthat mediates formation of this fascinating cell type. The\nresults show that a combination of transcriptional inputs\nand epigenetic modifiers control the timing of onset of\nneural crest gene expression. This in turn leads to the EMT\nprocess that produces this migratory cell population.", "date": "2012-08", "date_type": "published", "publication": "Histochemistry and Cell Biology", "volume": "138", "number": "2", "publisher": "Springer", "pagerange": "179-186", "id_number": "CaltechAUTHORS:20120928-095223938", "issn": "0948-6143", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120928-095223938", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1007/s00418-012-0999-z", "pmcid": "PMC3425661", "primary_object": { "basename": "nihms395640.pdf", "url": "https://authors.library.caltech.edu/records/8nzhy-pz130/files/nihms395640.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/kzazs-9xr14", "eprint_id": 33283, "eprint_status": "archive", "datestamp": "2023-08-22 06:15:29", "lastmod": "2023-10-18 18:57:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Fishwick-K-J", "name": { "family": "Fishwick", "given": "K. J." } }, { "id": "Kim-E", "name": { "family": "Kim", "given": "E." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "M. E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "ILF-3 is a regulator of the neural plate border marker Zic1 in chick embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; embryo; ILF-3; NF-90; neural plate border", "note": "\u00a9 2012 Wiley Periodicals, Inc. \n\nIssue published online: 17 Jul. 2012; Article first published online: 15 Jun. 2012; Accepted manuscript online: 25 May 2012 10:31AM EST; Manuscript Accepted: 20 May 2012. \n\nOriginal cDNA from library was cloned into pMES expression vector and the probe synthesized by S. McKeown. This work was funded by NIH grant HG004071 to Marianne E. Bronner.\n\nAccepted Version - nihms379901.pdf
", "abstract": "Background: The neural crest is a multipotent cell type unique to the vertebrate lineage and capable of differentiating into a large number of varied cell types, including ganglia of the peripheral nervous system, cartilage, and glia. An early step in neural crest specification occurs at the neural plate border, a region defined by the overlap of transcription factors of the Zic, Msx, and Pax families. Results: Here we identify a novel chick gene with close homology to double-stranded RNA-binding protein Interleukin enhancer binding factor 3 (ILF-3) in other species. Our results show that chick Ilf-3 is required for proper expression of the transcription factor, Zic-1, at the neural plate border. Conclusion: We have identified a novel chick gene and show it has a role in the correct specification of Zic-1 at the neural plate border.", "date": "2012-08", "date_type": "published", "publication": "Developmental Dynamics", "volume": "241", "number": "8", "publisher": "Wiley-Liss, Inc.", "pagerange": "1325-1332", "id_number": "CaltechAUTHORS:20120817-095005460", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120817-095005460", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HG004071" } ] }, "doi": "10.1002/dvdy.23809", "pmcid": "PMC3399927", "primary_object": { "basename": "nihms379901.pdf", "url": "https://authors.library.caltech.edu/records/kzazs-9xr14/files/nihms379901.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Fishwick, K. J.; Kim, E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3rcmv-59c94", "eprint_id": 35472, "eprint_status": "archive", "datestamp": "2023-08-19 11:23:01", "lastmod": "2023-10-20 16:23:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Uy-B-R", "name": { "family": "Uy", "given": "Benjamin R." }, "orcid": "0000-0003-0438-880X" }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Expression of Sox family genes in early lamprey development", "ispublished": "pub", "full_text_status": "public", "keywords": "Sox gene, lamprey, cyclostome, neural crest", "note": "\u00a9 2012 UBC Press. \n\nAccepted: 13 February 2012. Final, author-corrected PDF published online: 15 June 2012. \n\nWe thank Natalya Nikitina for her contribution of previously sequenced genes. This work was supported by NIH grant DE017911 to MEB.\n\nPublished - ft377.pdf
Supplemental Material - IntJDevBiol-113416bu-SuppMaterial.pdf
", "abstract": "Members of the Sox (Sry-related high mobility group box) family of transcription factors play a variety of roles during development of both vertebrates and invertebrates. A marked expansion in gene number occurred during the emergence of vertebrates, apparently via gene duplication events that are thought to have facilitated new functions. By screening a macroarrayed library as well as the lamprey genome, we have isolated genes of the Sox B, D, E and F subfamilies in the basal jawless vertebrate, lamprey. The expression patterns of all identified Sox genes were examined from gastrulation through early organogenesis (embryonic day 4-14), with particular emphasis on the neural crest, a vertebrate innovation. Coupled with phylogenetic analysis of these Sox genes, the results provide insight into gene duplication and divergence in paralog deployment occurring during early vertebrate evolution.", "date": "2012-06-15", "date_type": "published", "publication": "International Journal of Developmental Biology", "volume": "56", "number": "5", "publisher": "University of the Basque Country Press", "pagerange": "377-383", "id_number": "CaltechAUTHORS:20121115-084947443", "issn": "0214-6282", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121115-084947443", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1387/ijdb.113416bu", "pmcid": "PMC4118928", "primary_object": { "basename": "IntJDevBiol-113416bu-SuppMaterial.pdf", "url": "https://authors.library.caltech.edu/records/3rcmv-59c94/files/IntJDevBiol-113416bu-SuppMaterial.pdf" }, "related_objects": [ { "basename": "ft377.pdf", "url": "https://authors.library.caltech.edu/records/3rcmv-59c94/files/ft377.pdf" } ], "resource_type": "article", "pub_year": "2012", "author_list": "Uy, Benjamin R.; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/20jas-65087", "eprint_id": 32034, "eprint_status": "archive", "datestamp": "2023-08-19 11:16:12", "lastmod": "2023-10-17 22:22:47", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "LeDouarin-N-M", "name": { "family": "LeDouarin", "given": "Nicole M." } } ] }, "title": "Development and evolution of the neural crest: An overview", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2012 Elsevier Inc. \n\nAvailable online 2 January 2012. \n\nWe would like to acknowledge Les Treilles Foundation, whose\ngenerous support and wonderful atmosphere provided the participants of our Neural Crest study group with an environment that stimulated\ndiscussion and promoted fruitful interactions. We are particularly\ngrateful to Mme. Catherine Bachy, whose organizational assistance\ngreatly contributed to the success of our endeavor.\n\nPublished - Bronner2012p18600Dev_Biol.pdf
", "abstract": "The neural crest is a multipotent and migratory cell type that forms transiently in the developing vertebrate embryo. These cells emerge from the central nervous system, migrate extensively and give rise to diverse cell lineages including melanocytes, craniofacial cartilage and bone, peripheral and enteric neurons and glia, and smooth muscle. A vertebrate innovation, the gene regulatory network underlying neural crest formation appears to be highly conserved, even to the base of vertebrates. Here, we present an overview of important concepts in the neural crest field dating from its discovery 150 years ago to open questions that will motivate future research.", "date": "2012-06-01", "date_type": "published", "publication": "Developmental Biology", "volume": "366", "number": "1", "publisher": "Elsevier", "pagerange": "2-9", "id_number": "CaltechAUTHORS:20120622-101407248", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120622-101407248", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2011.12.042", "pmcid": "PMC3351559", "primary_object": { "basename": "Bronner2012p18600Dev_Biol.pdf", "url": "https://authors.library.caltech.edu/records/20jas-65087/files/Bronner2012p18600Dev_Biol.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Bronner, Marianne E. and LeDouarin, Nicole M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4px9m-r8s39", "eprint_id": 63381, "eprint_status": "archive", "datestamp": "2023-08-19 11:16:59", "lastmod": "2023-10-25 23:46:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "Carole" } } ] }, "title": "Preface: the neural crest\u2014From stem cell formation to migration and differentiation", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2012 Elsevier.", "abstract": "A central question in developmental biology is: how does a complex organism develop from a single cell, the fertilized egg? The problem itself is so complex that investigators strive to find simpler systems in which to address this broad question. The vertebrate neural crest presents the opportunity to study a group of outwardly homogeneous cells that form in a discrete region of the body, at the edge of the forming central nervous. They subsequently leave their site of origin, undergo migrations that are sometimes very extensive, finally differentiating into a wide range of derivatives as disparate as neurons, pigment cells and cartilage.", "date": "2012-06-01", "date_type": "published", "publication": "Developmental Biology", "volume": "366", "number": "1", "publisher": "Elsevier", "pagerange": "1", "id_number": "CaltechAUTHORS:20160105-123345536", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160105-123345536", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.ydbio.2012.03.011", "resource_type": "article", "pub_year": "2012", "author_list": "Bronner, Marianne E. and LaBonne, Carole" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tmnaj-n3z88", "eprint_id": 31689, "eprint_status": "archive", "datestamp": "2023-08-22 05:33:27", "lastmod": "2023-10-17 18:46:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kerosuo-Laura", "name": { "family": "Kerosuo", "given": "Laura" }, "orcid": "0000-0001-6710-3512" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "What is bad in cancer is good in the embryo: Importance of EMT in neural crest development", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; EMT; Apicobasal polarity; Dorsal neural tube; Snail", "note": "\u00a9 2012 Elsevier Ltd. \n\nAvailable online 10 March 2012. \n\nThis work was funded by grants from Sigrid Juselius Foundation, K. Albin Johansson Foundation and Ella and Georg Ehrnrooth Foundation to LK and NIH grants DE017911 and HD037105 to MEB.\n\nAccepted Version - nihms368137.pdf
", "abstract": "Although the epithelial to mesenchymal transition (EMT) is famous for its role in cancer metastasis, it also is a normal developmental event in which epithelial cells are converted into migratory mesenchymal cells. A prime example of EMT during development occurs when neural crest (NC) cells emigrate from the neural tube thus providing an excellent model to study the principles of EMT in a nonmalignant environment. NC cells start life as neuroepithelial cells intermixed with precursors of the central nervous system. After EMT, they delaminate and begin migrating, often to distant sites in the embryo. While proliferating and maintaining multipotency and cell survival the transitioning neural crest cells lose apicobasal polarity and the basement membrane is broken down. This review discusses how these events are coordinated and regulated, by series of events involving signaling factors, gene regulatory interactions, as well as epigenetic and post-transcriptional modifications. Even though the series of events involved in NC EMT are well known, the sequence in which these steps take place remains a subject of debate, raising the intriguing possibility that, rather than being a single event, neural crest EMT may involve multiple parallel mechanisms.", "date": "2012-05", "date_type": "published", "publication": "Seminars in Cell and Developmental Biology", "volume": "23", "number": "3", "publisher": "Elsevier", "pagerange": "320-332", "id_number": "CaltechAUTHORS:20120530-072836749", "issn": "1084-9521", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120530-072836749", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Sigrid Juselius Foundation" }, { "agency": "K. Albin Johansson Foundation" }, { "agency": "Ella and Georg Ehrnrooth Foundation" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1016/j.semcdb.2012.03.010", "pmcid": "PMC3345076", "primary_object": { "basename": "nihms368137.pdf", "url": "https://authors.library.caltech.edu/records/tmnaj-n3z88/files/nihms368137.pdf" }, "resource_type": "article", "pub_year": "2012", "author_list": "Kerosuo, Laura and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/r24kx-m7x75", "eprint_id": 31671, "eprint_status": "archive", "datestamp": "2023-08-19 09:26:17", "lastmod": "2023-10-17 18:45:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Rogers-C-D", "name": { "family": "Rogers", "given": "C. D." }, "orcid": "0000-0002-9549-1089" }, { "id": "Jayasena-C-S", "name": { "family": "Jayasena", "given": "C. S." } }, { "id": "Nie-S", "name": { "family": "Nie", "given": "S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest specification: tissues, signals, and transcription factors", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2011 Wiley Periodicals, Inc. \n\nIssue published online: 14 Dec. 2011. Article first published online: 17 Nov. 2011.", "abstract": "The neural crest is a transient population of multipotent and migratory cells unique to vertebrate embryos. Initially derived from the borders of the neural plate, these cells undergo an epithelial to mesenchymal transition to leave the central nervous system, migrate extensively in the periphery, and differentiate into numerous diverse derivatives. These include but are not limited to craniofacial cartilage, pigment cells, and peripheral neurons and glia. Attractive for their similarities to stem cells and metastatic cancer cells, neural crest cells are a popular model system for studying cell/tissue interactions and signaling factors that influence cell fate decisions and lineage transitions. In this review, we discuss the mechanisms required for neural crest formation in various vertebrate species, focusing on the importance of signaling factors from adjacent tissues and conserved gene regulatory interactions, which are required for induction and specification of the ectodermal tissue that will become neural crest.", "date": "2012-01", "date_type": "published", "publication": "Wiley Interdisciplinary Reviews: Developmental Biology", "volume": "1", "number": "1", "publisher": "John Wiley & Sons", "pagerange": "52-68", "id_number": "CaltechAUTHORS:20120529-091603681", "issn": "1759-7692", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120529-091603681", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/wdev.8", "resource_type": "article", "pub_year": "2012", "author_list": "Rogers, C. D.; Jayasena, C. S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7ar9y-rcg24", "eprint_id": 29232, "eprint_status": "archive", "datestamp": "2023-08-19 09:02:16", "lastmod": "2023-10-24 22:00:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Parker-H-J", "name": { "family": "Parker", "given": "Hugo J." }, "orcid": "0000-0001-7646-2007" }, { "id": "Piccinelli-P", "name": { "family": "Piccinelli", "given": "Paul" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Elgar-G", "name": { "family": "Elgar", "given": "Greg" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers", "ispublished": "pub", "full_text_status": "public", "keywords": "Gene regulation, enhancer code, sea lamprey, Hox genes, embryogenesis", "note": "\u00a9 2011 Parker et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons\nAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in\nany medium, provided the original work is properly cited.\n\nReceived: 7 October 2011; Accepted: 30 December 2011;\nPublished: 30 December 2011.\n\nWe are indebted to Andrew McCallion and Koichi Kawakami for supplying\nus with the pGW_cfosEGFP construct, and Reinhard Koester for the r3r5\ntransgenic zebrafish line. We thank Stefan Pauls and Debbie Goode for\nhelpful suggestions on the manuscript. This work was funded by MRC\nProject Grant 72504 to GE and NIH Grant DE017911 to MB. HJP was funded\nby a QMUL PhD studentship.\nAuthors' contributions:\nConceived and designed the experiments: HJP and GE. Performed the\nexperiments: HJP. Conceived and designed bio-informatic analyses: PP, HJP\nand GE. Performed bio-informatic analyses: PP and GE. Analysed the data:\nHJP, PP and GE. Supplied materials, reagents and lamprey expertise: MB and\nTS-S. Wrote the paper: HJP, PP and GE. All authors read and approved the\nfinal manuscript.\n\nPublished - Parker2011p17088Bmc_Genomics.pdf
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", "abstract": "Background:\n \nGene regulation through cis-regulatory elements plays a crucial role in development and disease. A major aim of the post-genomic era is to be able to read the function of cis-regulatory elements through scrutiny of their DNA sequence. Whilst comparative genomics approaches have identified thousands of putative regulatory elements, our knowledge of their mechanism of action is poor and very little progress has been made in systematically de-coding them.\n \nResults:\n \nHere, we identify ancient functional signatures within vertebrate conserved non-coding elements (CNEs) through a combination of phylogenetic footprinting and functional assay, using genomic sequence from the sea lamprey as a reference. We uncover a striking enrichment within vertebrate CNEs for conserved binding-site motifs of the Pbx-Hox hetero-dimer. We further show that these predict reporter gene expression in a segment specific manner in the hindbrain and pharyngeal arches during zebrafish development.\n \nConclusions:\n \nThese findings evoke an evolutionary scenario in which many CNEs evolved early in the vertebrate lineage to co-ordinate Hox-dependent gene-regulatory interactions that pattern the vertebrate head. In a broader context, our evolutionary analyses reveal that CNEs are composed of tightly linked transcription-factor binding-sites (TFBSs), which can be systematically identified through phylogenetic footprinting approaches. By placing a large number of ancient vertebrate CNEs into a developmental context, our findings promise to have a significant impact on efforts toward de-coding gene-regulatory elements that underlie vertebrate development, and will facilitate building general models of regulatory element evolution.", "date": "2011-12-30", "date_type": "published", "publication": "BMC Genomics", "volume": "12", "publisher": "BioMed Central", "pagerange": "Art. No. 637", "id_number": "CaltechAUTHORS:20120210-094550755", "issn": "1471-2164", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120210-094550755", "rights": "This is an Open Access article distributed under the terms of the Creative Commons\nAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in\nany medium, provided the original work is properly cited.", "funders": { "items": [ { "agency": "MRC Project Grant", "grant_number": "72504" }, { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "QMUL PhD studentship" } ] }, "doi": "10.1186/1471-2164-12-637", "primary_object": { "basename": "Additional_file_8.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_8.txt" }, "related_objects": [ { "basename": "Additional_file_7.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_7.txt" }, { "basename": "Additional_file_9.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_9.txt" }, { "basename": "Additional_file_10.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_10.txt" }, { "basename": "Additional_file_3.doc", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_3.doc" }, { "basename": "Additional_file_6.doc", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_6.doc" }, { "basename": "Additional_file_11.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_11.txt" }, { "basename": "Additional_file_12.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_12.txt" }, { "basename": "Additional_file_2.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_2.txt" }, { "basename": "Additional_file_5.doc", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_5.doc" }, { "basename": "Parker2011p17088Bmc_Genomics.pdf", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Parker2011p17088Bmc_Genomics.pdf" }, { "basename": "Additional_file_1.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_1.txt" }, { "basename": "Additional_file_13.txt", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_13.txt" }, { "basename": "Additional_file_4.doc", "url": "https://authors.library.caltech.edu/records/7ar9y-rcg24/files/Additional_file_4.doc" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Parker, Hugo J.; Piccinelli, Paul; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/skdhs-bze20", "eprint_id": 27984, "eprint_status": "archive", "datestamp": "2023-08-22 04:06:25", "lastmod": "2023-10-24 17:33:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Trihn-Le-A", "name": { "family": "Trihn", "given": "Le A." } }, { "id": "Hochgreb-T", "name": { "family": "Hochgreb", "given": "Tatiana" } }, { "id": "Graham-M-J", "name": { "family": "Graham", "given": "Matthew" }, "orcid": "0000-0002-3168-0139" }, { "id": "Wu-David", "name": { "family": "Wu", "given": "David" } }, { "id": "Ruf-Zamojski-F", "name": { "family": "Ruf-Zamojski", "given": "Frederique" } }, { "id": "Jayasena-C-S", "name": { "family": "Jayasena", "given": "Chathurani S." } }, { "id": "Saxena-Ankur", "name": { "family": "Saxena", "given": "Ankur" }, "orcid": "0000-0001-8646-2887" }, { "id": "Hawk-R", "name": { "family": "Hawk", "given": "Rasheeda" } }, { "id": "Gonzales-Serricchio-A", "name": { "family": "Gonzales-Serricchio", "given": "Aidyl" } }, { "id": "Dixson-A", "name": { "family": "Dixson", "given": "Alana" } }, { "id": "Chow-Elly", "name": { "family": "Chow", "given": "Elly" } }, { "id": "Gonzales-C", "name": { "family": "Gonzales", "given": "Constanza" } }, { "id": "Leung-Ho-Yin", "name": { "family": "Leung", "given": "Ho-Yin" } }, { "id": "Solomon-I", "name": { "family": "Solomon", "given": "Ilana" } }, { "id": "Megason-S-G", "name": { "family": "Megason", "given": "Sean G." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A versatile gene trap to visualize and interrogate the function of the vertebrate proteome", "ispublished": "pub", "full_text_status": "public", "keywords": "endogenous proteins; proteome; conditional mutagenesis; gene trap; genetic manipulation; gene knockdown", "note": "\u00a9 2011 by Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). \n\nReceived July 7, 2011; revised version accepted September 16, 2011. \n\nWe thank P. Pantazis for comments on the manuscript, and\nmembers of the Fraser laboratory for helpful discussions. We are grateful to LeighAnn Fletcher for fish care. The T2KXIGd-in and pMDS-eGFP plasmids were provided by K. Kawakami and S. Parinov, respectively. This work was supported by NHGRI Center of Excellence in Genomic Science grant P50HG004071.\n\nPublished - Genes_Dev.-2011-Trinh-2306-20.pdf
Published - Trinh2011p16329Genes_Dev.pdf
Supplemental Material - Binder2.pdf
Supplemental Material - ResourcePaperSI_text.pdf
", "abstract": "We report a multifunctional gene-trapping approach, which generates full-length Citrine fusions with endogenous proteins and conditional mutants from a single integration event of the FlipTrap vector. We identified 170 FlipTrap zebrafish lines with diverse tissue-specific expression patterns and distinct subcellular localizations of fusion proteins generated by the integration of an internal citrine exon. Cre-mediated conditional mutagenesis is enabled by heterotypic lox sites that delete Citrine and \"flip\" in its place mCherry with a polyadenylation signal, resulting in a truncated fusion protein. Inducing recombination with Cerulean-Cre results in fusion proteins that often mislocalize, exhibit mutant phenotypes, and dramatically knock down wild-type transcript levels. FRT sites in the vector enable targeted genetic manipulation of the trapped loci in the presence of Flp recombinase. Thus, the FlipTrap captures the functional proteome, enabling the visualization of full-length fluorescent fusion proteins and interrogation of function by conditional mutagenesis and targeted genetic manipulation.", "date": "2011-11-01", "date_type": "published", "publication": "Genes and Development", "volume": "25", "number": "21", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "2306-2320", "id_number": "CaltechAUTHORS:20111129-075143013", "issn": "0890-9369", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111129-075143013", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P50HG004071" } ] }, "doi": "10.1101/gad.174037.111", "pmcid": "PMC3219234", "primary_object": { "basename": "ResourcePaperSI_text.pdf", "url": "https://authors.library.caltech.edu/records/skdhs-bze20/files/ResourcePaperSI_text.pdf" }, "related_objects": [ { "basename": "Trinh2011p16329Genes_Dev.pdf", "url": "https://authors.library.caltech.edu/records/skdhs-bze20/files/Trinh2011p16329Genes_Dev.pdf" }, { "basename": "Binder2.pdf", "url": "https://authors.library.caltech.edu/records/skdhs-bze20/files/Binder2.pdf" }, { "basename": "Genes_Dev.-2011-Trinh-2306-20.pdf", "url": "https://authors.library.caltech.edu/records/skdhs-bze20/files/Genes_Dev.-2011-Trinh-2306-20.pdf" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Trihn, Le A.; Hochgreb, Tatiana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qd1v4-8hq92", "eprint_id": 28503, "eprint_status": "archive", "datestamp": "2023-08-22 04:03:48", "lastmod": "2023-10-24 17:56:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Jayasena-C-S", "name": { "family": "Jayasena", "given": "Chathurani S." } }, { "id": "Trinh-Le-A", "name": { "family": "Trinh", "given": "Le A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Pwp2h; zebrafish; ribosome biogenesis; development", "note": "\u00a9 2011 Wiley Periodicals, Inc. Accepted 30 August 2011.\nPublished online 26 September 2011. We thank Ho-Yin Leung for performing 30 RACE and LeighAnn Fletcher\nfor technical help in the Caltech Centers\nof Excellence in Genomic Science\nfish facility. This work is supported by\nUSPHS P50HG004071.", "abstract": "Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein-trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full-length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm-derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development.", "date": "2011-11", "date_type": "published", "publication": "Developmental Dynamics", "volume": "240", "number": "11", "publisher": "Wiley-Liss, Inc.", "pagerange": "2578-2583", "id_number": "CaltechAUTHORS:20111216-162340043", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111216-162340043", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P50HG004071" } ] }, "doi": "10.1002/dvdy.22744", "resource_type": "article", "pub_year": "2011", "author_list": "Jayasena, Chathurani S.; Trinh, Le A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g4029-jg884", "eprint_id": 28383, "eprint_status": "archive", "datestamp": "2023-08-19 08:27:19", "lastmod": "2023-10-24 17:51:19", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "H\u00e4ming-D", "name": { "family": "H\u00e4ming", "given": "Daniela" } }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Uy-B", "name": { "family": "Uy", "given": "Benjamin" } }, { "id": "Valencia-J-E", "name": { "family": "Valencia", "given": "Jonathan" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Expression of Sympathetic Nervous System Genes in Lamprey Suggests Their Recruitment for Specification of a New Vertebrate Feature", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2011 H\u00e4ming et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived April 29, 2011; Accepted September 28, 2011; Published October 27, 2011. \n\nEditor: Ferenc Mueller, University of Birmingham, United Kingdom. \n\nFunding: This work was supported by the National Institutes of Health (Grant #R01 DE017911). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. \n\nAuthor Contributions: Conceived and designed the experiments: MSC TSS MBF. Performed the experiments: DH MSC BU JV MBF. Analyzed the data: DH MSC TSS MBF. Wrote the paper: DH MSC MBF.\n\nPublished - Haeming2011p16433PLoS_ONE.pdf
", "abstract": "The sea lamprey is a basal, jawless vertebrate that possesses many neural crest derivatives, but lacks jaws and sympathetic ganglia. This raises the possibility that the factors involved in sympathetic neuron differentiation were either a gnathostome innovation or already present in lamprey, but serving different purposes. To distinguish between these possibilities, we isolated lamprey homologues of transcription factors associated with peripheral ganglion formation and examined their deployment in lamprey embryos. We further performed DiI labeling of the neural tube combined with neuronal markers to test if neural crest-derived cells migrate to and differentiate in sites colonized by sympathetic ganglia in jawed vertebrates. Consistent with previous anatomical data in adults, our results in lamprey embryos reveal that neural crest cells fail to migrate ventrally to form sympathetic ganglia, though they do form dorsal root ganglia adjacent to the neural tube. Interestingly, however, paralogs of the battery of transcription factors that mediate sympathetic neuron differentiation (dHand, Ascl1 and Phox2b) are present in the lamprey genome and expressed in various sites in the embryo, but fail to overlap in any ganglionic structures. This raises the intriguing possibility that they may have been recruited during gnathostome evolution to a new function in a neural crest derivative.", "date": "2011-10-27", "date_type": "published", "publication": "PLoS ONE", "volume": "6", "number": "10", "publisher": "Public Library of Science", "pagerange": "Art. No. e26543", "id_number": "CaltechAUTHORS:20111209-084941847", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111209-084941847", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE017911" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1371/journal.pone.0026543", "pmcid": "PMC3203141", "primary_object": { "basename": "Haeming2011p16433PLoS_ONE.pdf", "url": "https://authors.library.caltech.edu/records/g4029-jg884/files/Haeming2011p16433PLoS_ONE.pdf" }, "resource_type": "article", "pub_year": "2011", "author_list": "H\u00e4ming, Daniela; Sim\u00f5es-Costa, Marcos; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/c44sh-jmk62", "eprint_id": 27233, "eprint_status": "archive", "datestamp": "2023-08-22 03:46:41", "lastmod": "2023-10-24 16:58:53", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Tong-Leslie", "name": { "family": "Tong", "given": "Leslie" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ancestral Network Module Regulating prdm1 Expression in the Lamprey Neural Plate Border", "ispublished": "pub", "full_text_status": "public", "keywords": "prdm1; lamprey; neural crest; Blimp-1; MsxA; Ap-2; Pax7", "note": "\u00a9 2011 Wiley-Liss, Inc. \n\nIssue published online: 19 September 2011; Article first published online: 25 August 2011; Manuscript Accepted: 1 August 2011. \n\nThis work was funded by NIH grant DE017911 to M.E.B.\n\nAccepted Version - nihms352672.pdf
", "abstract": "prdm1 is an important transcriptional regulator that plays diverse roles during development of a wide variety of vertebrate and invertebrate species. prdm1 is required for neural crest specification in zebrafish, but not in mouse embryos. The role of this gene in neural crest formation in other species has not been examined, and its regulation during embryonic development is poorly understood. Here, we investigate the expression pattern, function, and the upstream regulatory inputs into prdm1 during lamprey neural crest development. prdm1 is strongly expressed in the lamprey neural plate border, suggesting a conserved ancestral role of this gene in the neural crest formation. We found that lamprey neural plate border expression of prdm1 is activated by Ap-2 and Msx, but is independent of Pax3/7 and Zic.", "date": "2011-10", "date_type": "published", "publication": "Developmental Dynamics", "volume": "240", "number": "10", "publisher": "Wiley-Liss, Inc.", "pagerange": "2265-2271", "id_number": "CaltechAUTHORS:20111014-122805711", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111014-122805711", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1002/dvdy.22720", "pmcid": "PMC3277493", "primary_object": { "basename": "nihms352672.pdf", "url": "https://authors.library.caltech.edu/records/c44sh-jmk62/files/nihms352672.pdf" }, "resource_type": "article", "pub_year": "2011", "author_list": "Nikitina, Natalya; Tong, Leslie; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hxpea-egp61", "eprint_id": 27119, "eprint_status": "archive", "datestamp": "2023-08-22 03:46:24", "lastmod": "2023-10-24 16:53:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Jayasena-C-S", "name": { "family": "Jayasena", "given": "Chathurani S." } }, { "id": "Trinh-Le-A", "name": { "family": "Trinh", "given": "Le A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Live imaging of endogenous Collapsin response mediator protein-1 expression at subcellular resolution during zebrafish nervous system development", "ispublished": "pub", "full_text_status": "public", "keywords": "Crmp1; Zebrafish; Nervous system; Development", "note": "\u00a9 2011 Elsevier B.V.\n\nReceived 2 March 2011; revised 16 May 2011; Accepted 17 May 2011. Available online 24 May 2011.\n\nWe wish to thank the following people: Ho-Yin Leung for performing 3' RACE and LeighAnn Fletcher for technical help in the Caltech Centers of Excellence in Genomic Science fish facility. This work is supported by USPHS P50HG004071.\n\nAccepted Version - nihms298668.pdf
", "abstract": "Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are functionally important during vertebrate development. We have generated a zebrafish gene trap line that produces fluorescently tagged Crmp1 protein, which can be dynamically tracked in living fish at subcellular resolution. The results show that Crmp1 is expressed in numerous sites in the developing nervous system. Early expression is apparent in the forebrain, epiphysis, optic tectum and the developing spinal cord. In the larval brain, Crmp1 is expressed in several distinct brain regions, such as the telencephalon, habenula and cerebellum. In addition, it is expressed in the spinal cord in a manner that persists in the larva. The results suggest that this Crmp1 protein trap line offers a powerful tool to track selected neuronal populations at high resolution.", "date": "2011-10", "date_type": "published", "publication": "Gene Expression Patterns", "volume": "11", "number": "7", "publisher": "Elsevier", "pagerange": "395-400", "id_number": "CaltechAUTHORS:20111007-100356026", "issn": "1567-133X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111007-100356026", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "P50HG004071" } ] }, "doi": "10.1016/j.gep.2011.05.002", "pmcid": "PMC3163798", "primary_object": { "basename": "nihms298668.pdf", "url": "https://authors.library.caltech.edu/records/hxpea-egp61/files/nihms298668.pdf" }, "resource_type": "article", "pub_year": "2011", "author_list": "Jayasena, Chathurani S.; Trinh, Le A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6wfdn-bq053", "eprint_id": 25467, "eprint_status": "archive", "datestamp": "2023-08-19 08:04:01", "lastmod": "2023-10-24 15:53:51", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" } }, { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Caldesmon regulates actin dynamics to influence cranial neural crest migration in Xenopus", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2011 Nie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution\u2013Noncommercial\u2013Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). \n\nSubmitted February 25, 2011. Revised July 15, 2011. Accepted July 21, 2011. Published online before print July 27, 2011. \n\nThis work was supported by DE017911 and HD037105 from the National Institutes of Health to M.B.F. and by a 2011 Research Grant from Kangwon National University to Y.K.\n\nPublished - Nie2011p15883Mol_Biol_Cell.pdf
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", "abstract": "Caldesmon (CaD) is an important actin modulator that associates with actin filaments to regulate cell morphology and motility. Although extensively studied in cultured cells, there is little functional information regarding the role of CaD in migrating cells in vivo. Here we show that nonmuscle CaD is highly expressed in both premigratory and migrating cranial neural crest cells of Xenopus embryos. Depletion of CaD with antisense morpholino oligonucleotides causes cranial neural crest cells to migrate a significantly shorter distance, prevents their segregation into distinct migratory streams, and later results in severe defects in cartilage formation. Demonstrating specificity, these effects are rescued by adding back exogenous CaD. Interestingly, CaD proteins with mutations in the Ca^(2+)-calmodulin\u2013binding sites or ErK/Cdk1 phosphorylation sites fail to rescue the knockdown phenotypes, whereas mutation of the PAK phosphorylation site is able to rescue them. Analysis of neural crest explants reveals that CaD is required for the dynamic arrangements of actin and, thus, for cell shape changes and process formation. Taken together, these results suggest that the actin-modulating activity of CaD may underlie its critical function and is regulated by distinct signaling pathways during normal neural crest migration.", "date": "2011-09-15", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "22", "number": "18", "publisher": "American Society for Cell Biology", "pagerange": "3355-3365", "id_number": "CaltechAUTHORS:20110928-101432580", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110928-101432580", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "Kangwon National University of Y. K." } ] }, "doi": "10.1091/mbc.E11-02-0165", "pmcid": "PMC3172261", "primary_object": { "basename": "CombinedSupMats.pdf", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/CombinedSupMats.pdf" }, "related_objects": [ { "basename": "Nie2011p15883Mol_Biol_Cell.pdf", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/Nie2011p15883Mol_Biol_Cell.pdf" }, { "basename": "mc-E11-02-0165-s04.mov", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/mc-E11-02-0165-s04.mov" }, { "basename": "mc-E11-02-0165-s05.mov", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/mc-E11-02-0165-s05.mov" }, { "basename": "mc-E11-02-0165-s06.mov", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/mc-E11-02-0165-s06.mov" }, { "basename": "mc-E11-02-0165-s07.mov", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/mc-E11-02-0165-s07.mov" }, { "basename": "mc-E11-02-0165-s08.mov", "url": "https://authors.library.caltech.edu/records/6wfdn-bq053/files/mc-E11-02-0165-s08.mov" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Nie, Shuyi; Kee, Yun; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9qa1r-anj34", "eprint_id": 25120, "eprint_status": "archive", "datestamp": "2023-08-22 03:36:31", "lastmod": "2023-10-24 15:38:27", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Betancur-P", "name": { "family": "Betancur", "given": "Paola" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A Sox10 enhancer element common to the otic placode and\n neural crest is activated by tissue-specific paralogs", "ispublished": "pub", "full_text_status": "public", "keywords": "Ear; Cis-regulation; Sox10; Chick", "note": "\u00a9 2011 The Company of Biologists Ltd.\n\nAccepted 20 June 2011.\nPublished online before print July 20, 2011.\nWe thank H. Kondoh for the pTK-EGFP reporter construct; Y. C. Cheng for full length Sox10; V. Lee for full length cSox8; C. LaBonne for XSox9K61R,365R; J.-P. Saint-Jeannet for full length XSox8; M. Dvorak for the cMyb antibody; M.\nJones and J. Tan for technical help; M. Barembaum for full length cPea3; and C. Baker for helpful discussions. This project was supported by NIH grants HD037105 and DE16459. Deposited in PMC for release after 12 months.\n\nPublished - Betancur2011p15601Development.pdf
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", "abstract": "The otic placode, a specialized region of ectoderm, gives rise to components of the inner ear and shares many characteristics with the neural crest, including expression of the key transcription factor Sox10. Here, we show that in avian embryos, a highly conserved cranial neural crest enhancer, Sox10E2, also controls the onset of Sox10 expression in the otic placode. Interestingly, we show that different combinations of paralogous transcription factors (Sox8, Pea3 and cMyb versus Sox9, Ets1 and cMyb) are required to mediate Sox10E2 activity in the ear and neural crest, respectively. Mutating their binding motifs within Sox10E2 greatly reduces enhancer activity in the ear. Moreover, simultaneous knockdown of Sox8, Pea3 and cMyb eliminates not only the enhancer-driven reporter expression, but also the onset of endogenous Sox10 expression in the ear. Rescue experiments confirm that the specific combination of Myb together with Sox8 and Pea3 is responsible for the onset of Sox10 expression in the otic placode, as opposed to Myb plus Sox9 and Ets1 for neural crest Sox10 expression. Whereas SUMOylation of Sox8 is not required for the initial onset of Sox10 expression, it is necessary for later otic vesicle formation. This new role of Sox8, Pea3 and cMyb in controlling Sox10 expression via a common otic/neural crest enhancer suggests an evolutionarily conserved function for the combination of paralogous transcription factors in these tissues of distinct embryological origin.", "date": "2011-09-01", "date_type": "published", "publication": "Development", "volume": "138", "number": "17", "publisher": "Company of Biologists", "pagerange": "3689-3698", "id_number": "CaltechAUTHORS:20110826-104710211", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110826-104710211", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1242/dev.057836", "primary_object": { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/9qa1r-anj34/files/medium.png" }, "related_objects": [ { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/9qa1r-anj34/files/small.png" }, { "basename": "Betancur2011p15601Development.pdf", "url": "https://authors.library.caltech.edu/records/9qa1r-anj34/files/Betancur2011p15601Development.pdf" }, { "basename": "DEV057836FigS1.jpg", "url": "https://authors.library.caltech.edu/records/9qa1r-anj34/files/DEV057836FigS1.jpg" }, { "basename": "DEV057836FigS2.jpg", "url": "https://authors.library.caltech.edu/records/9qa1r-anj34/files/DEV057836FigS2.jpg" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Betancur, Paola; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3ahe7-1zq11", "eprint_id": 24005, "eprint_status": "archive", "datestamp": "2023-08-19 06:29:39", "lastmod": "2023-10-23 20:14:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Cimadamore-F", "name": { "family": "Cimadamore", "given": "Flavio" } }, { "id": "Fishwick-K-J", "name": { "family": "Fishwick", "given": "Katherine" } }, { "id": "Giusto-E", "name": { "family": "Giusto", "given": "Elena" } }, { "id": "Gnedeva-K", "name": { "family": "Gnedeva", "given": "Ksenia" } }, { "id": "Cattarossi-G", "name": { "family": "Cattarossi", "given": "Giulio" } }, { "id": "Miller-A", "name": { "family": "Miller", "given": "Amber" } }, { "id": "Pluchino-S", "name": { "family": "Pluchino", "given": "Stefano" } }, { "id": "Brill-L-M", "name": { "family": "Brill", "given": "Lawrence M." } }, { "id": "Terskikh-A-V", "name": { "family": "Terskikh", "given": "Alexey V." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Human ESC-Derived Neural Crest Model Reveals a Key Role for SOX2 in Sensory Neurogenesis", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2011 Elsevier Inc. \n\nReceived 16 July 2010; revised 26 January 2011; accepted 4 March 2011. Published: May 5, 2011. Available online 5 May 2011. \n\nWe thank C.-T. Huang and K. Liu for their help with cloning, lentiviral production, and microarray analysis. We thank Dr. S. Albini, Dr. S. Forcales, and Professor L. Puri for sharing their expertise in chromatin immunoprecipitation techniques. Sox2LoxP mice were kindly provided by Dr. Nicolis, and Wnt1-CRE mice were kindly provided by Dr. Y. Yamaguchi. We thank Dr. J. Hou for helping with IP-MS data analysis. This work has been supported by CIRM postdoctoral fellowship to F.C., CIRM grant RS1004661 to A.T., and transient research support to A.V. Terskikh from the Sanford-Burnham Medical Research Institute and an NIH Blueprint core grant (PI, S.A. Lipton).\n\nAccepted Version - nihms601413.pdf
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", "abstract": "The transcription factor SOX2 is widely known to play a critical role in the central nervous system; however, its role in peripheral neurogenesis remains poorly understood. We recently developed an hESC-based model in which migratory cells undergo epithelial to mesenchymal transition (EMT) to acquire properties of neural crest (NC) cells. In this model, we found that migratory NC progenitors downregulate SOX2, but then start re-expressing SOX2 as they differentiate to form neurogenic dorsal root ganglion (DRG)-like clusters. SOX2 downregulation was sufficient to induce EMT and resulted in massive apoptosis when neuronal differentiation was induced. In vivo, downregulation of SOX2 in chick and mouse NC cells significantly reduced the numbers of neurons within DRG. We found that SOX2 binds directly to NGN1 and MASH1 promoters and is required for their expression. Our data suggest that SOX2 plays a key role for NGN1-dependent acquisition of neuronal fates in sensory ganglia.", "date": "2011-05-06", "date_type": "published", "publication": "Cell Stem Cell", "volume": "8", "number": "5", "publisher": "Elsevier", "pagerange": "538-551", "id_number": "CaltechAUTHORS:20110614-133159726", "issn": "1934-5909", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110614-133159726", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "RS1004661" }, { "agency": "Sanford-Burnham Medical Research Institute" }, { "agency": "NIH" } ] }, "doi": "10.1016/j.stem.2011.03.011", "pmcid": "PMC4110917", "primary_object": { "basename": "mmc1.doc", "url": "https://authors.library.caltech.edu/records/3ahe7-1zq11/files/mmc1.doc" }, "related_objects": [ { "basename": "nihms601413.pdf", "url": "https://authors.library.caltech.edu/records/3ahe7-1zq11/files/nihms601413.pdf" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Cimadamore, Flavio; Fishwick, Katherine; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/n5x5c-zg872", "eprint_id": 23382, "eprint_status": "archive", "datestamp": "2023-08-22 02:21:34", "lastmod": "2023-10-23 19:03:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Tetraspanin, CD151, is required for maintenance of trigeminal placode identity", "ispublished": "pub", "full_text_status": "restricted", "keywords": "CD151; neuron; Pax3; specification; trigeminal placode", "note": "\u00a9 2011 The Authors. Journal of Neurochemistry. \u00a9 2011 International Society for Neurochemistry. Received October 13, 2010; revised manuscript received December 10,\n2010; accepted January 6, 2011. Article first published online: 24 Feb. 2011. We would like to thank Samuel Ki for his technical assistance. This\nwork was supported by DE16459 to MBF. The authors have no\ncompeting interests.", "abstract": "The trigeminal ganglion is the largest of the cranial ganglia and responsible for transmitting sensory information for much of the face. The cell surface glycoprotein CD151 is an early marker of the trigeminal placode, the precursor to the ganglion. Here, we investigate the role of CD151 during specification of trigeminal placode cells in the developing chicken embryo. Expression of the transcription factor Pax3, the earliest known marker of the trigeminal placode, briefly precedes that of CD151, but they then subsequently overlap in the trigeminal placode. Loss of CD151 protein dramatically decreases the number of Pax3+ placode cells in Stage 13\u201314 embryos, leading to loss of ophthalmic trigeminal neurons by Stages 16 and 17. Although the initial size of the Pax3 population is similar to that in controls, the number of Pax3+ cells decreases with time without alterations in cell death or proliferation. This suggests a role for CD151 in maintenance of the specification state in the trigeminal placode, uncovering the first known role for a tetraspanin in a developmental system.", "date": "2011-04", "date_type": "published", "publication": "Journal of Neurochemistry", "volume": "117", "number": "2", "publisher": "Blackwell Publishing", "pagerange": "221-230", "id_number": "CaltechAUTHORS:20110419-112632632", "issn": "0022-3042", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110419-112632632", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1111/j.1471-4159.2011.07190.x", "resource_type": "article", "pub_year": "2011", "author_list": "McCabe, Kathryn L. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9kvm4-cfp29", "eprint_id": 22582, "eprint_status": "archive", "datestamp": "2023-08-22 01:50:50", "lastmod": "2023-10-23 17:01:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ezin-A-M", "name": { "family": "Ezin", "given": "Akouavi M." } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Zah-A", "name": { "family": "Zah", "given": "Angela" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Early regulative ability of the neuroepithelium to form cardiac neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Regeneration; Ablation; Cardiac neural crest; Cardiac outflow tract; Aorticopulmonary septum; Regulation", "note": "\u00a9 2010 Elsevier Inc. \n\nReceived for publication 10 May 2010.\nRevised 26 October 2010. Accepted 26 October 2010. Available online 1 November 2010. \n\nWe would like to thank allmembers of the Bronner-Fraser and Fraser\nlabs. Thanks is extended to Aura Keeter for sectioning, antibody staining\nand lab support. We would also like to thank Peter Lwigale for training in\nmicrodissection. Katrin Wunnenberg-Stapleton and Simone Lutolf\nprovided excellent assistance with DiI labeling of control embryos. We\nthank Tamira Elul for productive discussions about data quantification.\nFinally, special thanks to Kathryn McCabe for assistance troubleshooting\nantibody staining procedures and Nicolas Plachta and Jennifer Yang for\nhelpwith live and static imaging, respectively. This work was funded by\nNIH grants HD037105 and NS36585 to M.B. and funding from the\nBeckman Institute to the Biological Imaging Center.\n\nAccepted Version - nihms254445.pdf
", "abstract": "The cardiac neural crest (arising from the level of hindbrain rhombomeres 6\u20138) contributes to the septation of\nthe cardiac outflow tract and the formation of aortic arches. Removal of this population after neural tube\nclosure results in severe septation defects in the chick, reminiscent of human birth defects. Because neural\ncrest cells from other axial levels have regenerative capacity, we asked whether the cardiac neural crest might\nalso regenerate at early stages in a manner that declines with time. Accordingly, we find that ablation of\npresumptive cardiac crest at stage 7, as the neural folds elevate, results in reformation of migrating cardiac\nneural crest by stage 13. Fate mapping reveals that the new population derives largely from the\nneuroepithelium ventral and rostral to the ablation. The stage of ablation dictates the competence of residual\ntissue to regulate and regenerate, as this capacity is lost by stage 9, consistent with previous reports. These\nfindings suggest that there is a temporal window during which the presumptive cardiac neural crest has the\ncapacity to regulate and regenerate, but this regenerative ability is lost earlier than in other neural crest\npopulations.", "date": "2011-01-15", "date_type": "published", "publication": "Developmental Biology", "volume": "349", "number": "2", "publisher": "Elsevier", "pagerange": "238-249", "id_number": "CaltechAUTHORS:20110301-113228567", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110301-113228567", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "Caltech Beckman Institute" } ] }, "doi": "10.1016/j.ydbio.2010.10.032", "pmcid": "PMC3018664", "primary_object": { "basename": "nihms254445.pdf", "url": "https://authors.library.caltech.edu/records/9kvm4-cfp29/files/nihms254445.pdf" }, "resource_type": "article", "pub_year": "2011", "author_list": "Ezin, Akouavi M.; Sechrist, John W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bhkcc-5mt08", "eprint_id": 22431, "eprint_status": "archive", "datestamp": "2023-08-22 01:49:40", "lastmod": "2023-10-23 16:01:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sobreira-T-J-P", "name": { "family": "Sobreira", "given": "Tiago J. P." } }, { "id": "Marl\u00e9taz-F", "name": { "family": "Marl\u00e9taz", "given": "Ferdinand" } }, { "id": "Sim\u00f5es-Costa-M-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos" }, "orcid": "0000-0003-1452-7068" }, { "id": "Schechtman-D", "name": { "family": "Schechtman", "given": "Deborah" } }, { "id": "Pereira-A-C", "name": { "family": "Pereira", "given": "Alexandre C." } }, { "id": "Brunet-F", "name": { "family": "Brunet", "given": "Fr\u00e9d\u00e9ric" } }, { "id": "Sweeney-S", "name": { "family": "Sweeney", "given": "Sarah" } }, { "id": "Pani-A", "name": { "family": "Pani", "given": "Ariel" } }, { "id": "Aronowicz-J", "name": { "family": "Aronowicz", "given": "Jochanan" } }, { "id": "Lowe-C-J", "name": { "family": "Lowe", "given": "Christopher J." } }, { "id": "Davidson-B", "name": { "family": "Davidson", "given": "Bradley" } }, { "id": "Laudet-V", "name": { "family": "Laudet", "given": "Vincent" } }, { "id": "Schubert-M", "name": { "family": "Schubert", "given": "Michael" } }, { "id": "de-Oliveira-P-S-L", "name": { "family": "de Oliveira", "given": "Paulo S. L." } }, { "id": "Xavier-Neto-J", "name": { "family": "Xavier-Neto", "given": "Jos\u00e9" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Structural shifts of aldehyde dehydrogenase enzymes were instrumental for the early evolution of retinoiddependent\n axial patterning in metazoans", "ispublished": "pub", "full_text_status": "public", "keywords": "Aldehyde dehydrogenase phylogeny; Branchiostoma floridae; Ciona intestinalis versus Ciona savignyi; evolution of retinoic acid signaling; origins of morphogen-dependent signaling", "note": "\u00a9 2011 National Academy of Sciences.\n\nEdited by John Gerhart, University of California, Berkeley, CA, and approved November 10, 2010 (received for review August 17, 2010).\nPublished online before print December 17, 2010.\nWe thank G\u00e9rard Benoit, Tiago Pereira, Linda Z. Holland, and Nicholas D. Holland for critical reading of the manuscript. We are indebted to the Faculty of Medicine of the University of S\u00e3o Paulo for access to its high-performance computing cluster. This work was supported\nby Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo Grant 06/50843-0 (to J.X.-N.), by funds from Agence Nationale de Recherche (ANR-07-BLAN-0038 and ANR-09-BLAN-0262-02), Centre National de la Recherche Scientifique, and Ministere de l'Education Nationale de la Recherche et de Technologie (to M.S.), and by the Consortium for Research into Nuclear Receptors in Development and Aging (CRESCENDO), a European Union Integrated Project of FP6. M.S.-C. was supported by a travel fellowship from the Company of Biologists.\n\nAuthor contributions: M.S. and J.X.-N. designed research; T.J.P.S., F.M., M.S.-C., D.S., F.B.,\nS.S., A.P., J.A., C.J.L., B.D., P.S.L.d.O., M.S., and J.X.-N. performed research; D.S., C.J.L., B.D.,\nM.B., and P.S.L.d.O. contributed new reagents/analytic tools; T.J.P.S., F.M., M.S.-C., D.S.,\nA.C.P., F.B., S.S., A.P., J.A., C.J.L., B.D., V.L., P.S.L.d.O., M.S., and J.X.-N. analyzed data; and\nT.J.P.S., F.M., D.S., B.D., V.L., M.B., P.S.L.d.O., M.S., and J.X.-N. wrote the paper.\n\nPublished - Sobreira2011p12637P_Natl_Acad_Sci_Usa.pdf
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", "abstract": "Aldehyde dehydrogenases (ALDHs) catabolize toxic aldehydes and process the vitamin A-derived retinaldehyde into retinoic acid (RA), a small diffusible molecule and a pivotal chordate morphogen. In this study, we combine phylogenetic, structural, genomic, and developmental gene expression analyses to examine the evolutionary origins of ALDH substrate preference. Structural modeling reveals that processing of small aldehydes, such as acetaldehyde, by ALDH2, versus large aldehydes, including retinaldehyde, by ALDH1A is associated with small versus large substrate entry channels (SECs), respectively. Moreover, we show that metazoan ALDH1s and ALDH2s are members of a single ALDH1/2 clade and that during evolution, eukaryote ALDH1/2s often switched between large and small SECs after gene duplication, transforming constricted channels into wide opened ones and vice versa. Ancestral sequence reconstructions suggest that during the evolutionary emergence of RA signaling, the ancestral, narrow-channeled metazoan ALDH1/2 gave rise to large ALDH1 channels capable of accommodating bulky aldehydes, such as retinaldehyde, supporting the view that retinoid-dependent signaling arose from ancestral cellular detoxification mechanisms. Our analyses also indicate that, on a more restricted evolutionary scale, ALDH1 duplicates from invertebrate chordates (amphioxus and ascidian tunicates) underwent switches to smaller and narrower SECs. When combined with alterations in gene expression, these switches led to neofunctionalization from ALDH1-like roles in embryonic patterning to systemic, ALDH2-like roles, suggesting functional shifts from signaling to detoxification.", "date": "2011-01-04", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "108", "number": "1", "publisher": "National Academy of Sciences", "pagerange": "226-231", "id_number": "CaltechAUTHORS:20110222-134006824", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110222-134006824", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo", "grant_number": "06/50843-0" }, { "agency": "Agence Nationale de Recherche", "grant_number": "ANR- 07-BLAN-0038" }, { "agency": "Agence Nationale de Recherche", "grant_number": "ANR-09-BLAN-0262-02" }, { "agency": "Centre National de la Recherche Scientifique" }, { "agency": "Ministere de l'Education Nationale de la Recherche et de Technologie" }, { "agency": "Consortium for Research into Nuclear Receptors in Development and Aging (CRESCENDO)" }, { "agency": "Company of Biologists" } ] }, "doi": "10.1073/pnas.1011223108", "pmcid": "PMC3017150", "primary_object": { "basename": "sd01.xls", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/sd01.xls" }, "related_objects": [ { "basename": "sd02.xls", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/sd02.xls" }, { "basename": "sm01.avi", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/sm01.avi" }, { "basename": "sm02.avi", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/sm02.avi" }, { "basename": "sm03.avi", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/sm03.avi" }, { "basename": "Sobreira2011p12637P_Natl_Acad_Sci_Usa.pdf", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/Sobreira2011p12637P_Natl_Acad_Sci_Usa.pdf" }, { "basename": "pnas.201011223SI.pdf", "url": "https://authors.library.caltech.edu/records/bhkcc-5mt08/files/pnas.201011223SI.pdf" } ], "resource_type": "article", "pub_year": "2011", "author_list": "Sobreira, Tiago J. P.; Marl\u00e9taz, Ferdinand; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/gpzrv-3s460", "eprint_id": 21350, "eprint_status": "archive", "datestamp": "2023-08-22 01:31:38", "lastmod": "2023-10-20 23:57:58", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Shiau-Celia-E", "name": { "family": "Shiau", "given": "Celia E." }, "orcid": "0000-0002-9347-9158" }, { "id": "Hua-Na", "name": { "family": "Hua", "given": "Na" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Altering Glypican-1 levels modulates canonical Wnt signaling during trigeminal placode development", "ispublished": "pub", "full_text_status": "public", "keywords": "Glypican; Trigeminal ganglion; Placode; Wnt", "note": "\u00a9 2010 Published by Elsevier Inc. \n\nReceived 24 May 2010; revised 15 September 2010; accepted 21 September 2010. Available online 27 September 2010. \n\nWe thank Andy Groves for the pTOP-nDSRed2 plasmid and members\nof M.B.-F. lab for discussions and technical advice, in particular Meyer\nBarembaum, Tatjana Sauka-Spengler, and Pablo Strobl. This work was\nsupported by US National Institutes of Health (NIH) National Research\nService Award 5T32 GM07616 to C. E. S. and N.H. and NIH grant DE16459 to M.B.-F.\n\nAccepted Version - nihms-607349.pdf
Supplemental Material - Shiau2010p12139Dev_Biol_supp.doc
", "abstract": "Glypicans are conserved cell surface heparan sulfate proteoglycans expressed in a spatiotemporally regulated manner in many developing tissues including the nervous system. Here, we show that Glypican-1 (GPC1) is expressed by trigeminal placode cells as they ingress and contribute to trigeminal sensory neurons in the chick embryo. Either expression of full-length or truncated GPC1 in vivo causes defects in trigeminal gangliogenesis in a manner that requires heparan sulfate side chains. This leads to either abnormal placodal differentiation or organization, respectively, with near complete loss of the ophthalmic (OpV) trigeminal ganglion in the most severe cases after over-expression of full-length GPC1. Interestingly, modulating GPC1 alters levels of endogenous Wnt signaling activity in the forming trigeminal ganglion, as indicated by Wnt-reporter expression. Accordingly, GPC1 over-expression phenocopies Wnt inhibition in causing loss of OpV placodal neurons. Furthermore, increased Wnt activity rescues the effects of GPC1 over-expression. Taken together, these results suggest that appropriate levels of GPC1 are essential for proper regulation of canonical Wnt signaling during differentiation and organization of trigeminal placodal cells into ganglia.", "date": "2010-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "348", "number": "1", "publisher": "Elsevier", "pagerange": "107-118", "id_number": "CaltechAUTHORS:20101214-101441084", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20101214-101441084", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5T32 GM07616" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1016/j.ydbio.2010.09.017", "pmcid": "PMC4082824", "primary_object": { "basename": "Shiau2010p12139Dev_Biol_supp.doc", "url": "https://authors.library.caltech.edu/records/gpzrv-3s460/files/Shiau2010p12139Dev_Biol_supp.doc" }, "related_objects": [ { "basename": "nihms-607349.pdf", "url": "https://authors.library.caltech.edu/records/gpzrv-3s460/files/nihms-607349.pdf" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Shiau, Celia E.; Hua, Na; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6yveq-e5z02", "eprint_id": 21208, "eprint_status": "archive", "datestamp": "2023-08-19 04:23:35", "lastmod": "2023-10-20 23:50:53", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Curchoe-C-L", "name": { "family": "Curchoe", "given": "Carol Lynn" } }, { "id": "Maurer-J", "name": { "family": "Maurer", "given": "Jochen" } }, { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja J." } }, { "id": "Cattarossi-G", "name": { "family": "Cattarossi", "given": "Giulio" } }, { "id": "Cimadamore-F", "name": { "family": "Cimadamore", "given": "Flavio" } }, { "id": "Nilbratt-M", "name": { "family": "Nilbratt", "given": "Mats" } }, { "id": "Snyder-E-Y", "name": { "family": "Snyder", "given": "Evan Y." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Terskikh-A-V", "name": { "family": "Terskikh", "given": "Alexey V." } } ] }, "title": "Early Acquisition of Neural Crest Competence During hESCs Neuralization", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2010 Curchoe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived April 7, 2010; Accepted September 23, 2010; Published November 9, 2010. \n\nEditor: Joseph Najbauer, City of Hope National Medical Center, United States of America. \n\nFunded by California Institute for Regenerative Medicine Seed grant RS1-00466-1 to Alexey V. Terskikh. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. \n\nThe authors have declared that no competing interests exist. \n\nThe authors would like to thank A. Miller and J. Lesperance for the help with hESC cultures, R. Bajpai for RNA preparation, Y. Altman for assistance with FACS sorting, K. Liu for microarray and Q-PCR assistance, L. Creighton for help with the immunhistochemistry on chondrocytes, D. Arce and M. Jones for assistance with cryosectioning, M. Wegner and M. Fukuda for provision of antibodies, R. Gupta and M. Mercola for providing the DKK plasmid, T. Cheung for DKK media preparation, E. Chavez and A. Ryan for providing timed pregnant Ret.k- mice. \n\nAuthor Contributions: Conceived and designed the experiments: MBF AVT. Performed the experiments: CLC JM SM GC FC MN. Analyzed the data: SM ES MBF AVT. Contributed reagents/materials/analysis tools: ES MBF AVT. Wrote the paper: CLC JM AVT.\n\nPublished - Curchoe2010p11980PLoS_ONE.pdf
Supplemental Material - FigureS1.tif
Supplemental Material - FigureS2.tif
", "abstract": "Background: \nNeural crest stem cells (NCSCs) are a transient multipotent embryonic cell population that represents a defining characteristic of vertebrates. The neural crest (NC) gives rise to many derivatives including the neurons and glia of the sensory and autonomic ganglia of the peripheral nervous system, enteric neurons and glia, melanocytes, and the cartilaginous, bony and connective tissue of the craniofacial skeleton, cephalic neuroendocrine organs, and some heart vessels.\nMethodology/Principal Findings: \nWe present evidence that neural crest (NC) competence can be acquired very early when human embryonic stem cells (hESCs) are selectively neuralized towards dorsal neuroepithelium in the absence of feeder cells in fully defined conditions. When hESC-derived neurospheres are plated on fibronectin, some cells emigrate onto the substrate. These early migratory Neural Crest Stem Cells (emNCSCs) uniformly upregulate Sox10 and vimentin, downregulate N-cadherin, and remodel F-actin, consistent with a transition from neuroepithelium to a mesenchymal NC cell. Over 13% of emNCSCs upregulate CD73, a marker of mesenchymal lineage characteristic of cephalic NC and connexin 43, found on early migratory NC cells. We demonstrated that emNCSCs give rise in vitro to all NC lineages, are multipotent on clonal level, and appropriately respond to developmental factors. We suggest that human emNCSC resemble cephalic NC described in model organisms. Ex vivo emNCSCs can differentiate into neurons in Ret.k- mouse embryonic gut tissue cultures and transplanted emNCSCs incorporate into NC-derived structures but not CNS tissues in chick embryos.\nConclusions/Significance:\nThese findings will provide a framework for further studying early human NC development including the epithelial to mesenchymal transition during NC delamination.", "date": "2010-11-09", "date_type": "published", "publication": "PLoS ONE", "volume": "5", "number": "11", "publisher": "Public Library of Science", "pagerange": "Art. No. e13890", "id_number": "CaltechAUTHORS:20101207-091550174", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20101207-091550174", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "RS1-00466-1" } ] }, "doi": "10.1371/journal.pone.0013890", "pmcid": "PMC2976694", "primary_object": { "basename": "Curchoe2010p11980PLoS_ONE.pdf", "url": "https://authors.library.caltech.edu/records/6yveq-e5z02/files/Curchoe2010p11980PLoS_ONE.pdf" }, "related_objects": [ { "basename": "FigureS1.tif", "url": "https://authors.library.caltech.edu/records/6yveq-e5z02/files/FigureS1.tif" }, { "basename": "FigureS2.tif", "url": "https://authors.library.caltech.edu/records/6yveq-e5z02/files/FigureS2.tif" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Curchoe, Carol Lynn; Maurer, Jochen; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hsx81-g8s71", "eprint_id": 23005, "eprint_status": "archive", "datestamp": "2023-08-22 01:17:35", "lastmod": "2023-10-23 17:48:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Betancur-P", "name": { "family": "Betancur", "given": "Paola" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Assembling Neural Crest Regulatory Circuits into a Gene Regulatory Network", "ispublished": "pub", "full_text_status": "public", "keywords": "stem cell; transcription factors; cell migration; cis-regulation", "note": "\u00a9 2010 by Annual Reviews. \n\nFirst published online as a Review in Advance on June 29, 2010.\n\nAccepted Version - nihms-589906.pdf
", "abstract": "The neural crest is a multipotent stem cell\u2013like population that gives rise to a wide range of derivatives in the vertebrate embryo including elements of the craniofacial skeleton and peripheral nervous system as well as melanocytes. The neural crest forms in a series of regulatory steps that include induction and specification of the prospective neural crest territory\u2013neural plate border, specification of bona fide neural crest progenitors, and differentiation into diverse derivatives. These individual processes during neural crest ontogeny are controlled by regulatory circuits that can be assembled into a hierarchical gene regulatory network (GRN). Here we present an overview of the GRN that orchestrates the formation of cranial neural crest cells. Formulation of this network relies on information largely inferred from gene perturbation studies performed in several vertebrate model organisms. Our representation of the cranial neural crest GRN also includes information about direct regulatory interactions obtained from the cis-regulatory analyses performed to date, which increases the resolution of the architectural circuitry within the network.", "date": "2010-11", "date_type": "published", "publication": "Annual Review of Cell and Developmental Biology", "volume": "26", "publisher": "Annual Reviews", "pagerange": "581-603", "id_number": "CaltechAUTHORS:20110321-093844927", "issn": "1081-0706", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110321-093844927", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1146/annurev.cellbio.042308.113245", "pmcid": "PMC4040144", "primary_object": { "basename": "nihms-589906.pdf", "url": "https://authors.library.caltech.edu/records/hsx81-g8s71/files/nihms-589906.pdf" }, "resource_type": "article", "pub_year": "2010", "author_list": "Betancur, Paola; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t8p1t-9k243", "eprint_id": 20503, "eprint_status": "archive", "datestamp": "2023-08-22 01:05:19", "lastmod": "2023-10-20 23:07:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Cerny-R", "name": { "family": "Cerny", "given": "Robert" } }, { "id": "Cattell-M", "name": { "family": "Cattell", "given": "Maria" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Yu-Feiqiao", "name": { "family": "Yu", "given": "Feiqiao" } }, { "id": "Meulemans-Medeiros-D", "name": { "family": "Meulemans Medeiros", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evidence for the prepattern/cooption model of vertebrate jaw evolution", "ispublished": "pub", "full_text_status": "public", "keywords": "vertebrate; jaw; evolution; pharynx; lamprey", "note": "\u00a9 2010 by the National Academy of Sciences. \n\nEdited by Clifford J. Tabin, Harvard Medical School, Boston, MA, and approved August 31, 2010 (received for review July 2, 2010). \n\nWe thank Roger Bergstedt, Deborah Winkler, Nikolas Rewald, and Kathy Jones (Hammond Bay Biological Station, Millersburg, MI) for generously supplying adult lampreys; Gage Crump and David Stock for critical reading of the manuscript; and two anonymous reviewers whose comments helped improve this work. R.C. was supported by the Grant Agency of the Academy of Sciences of the Czech Republic (206/07/P257) and by Ministry of Education, Youth, and Sports Project 0021620828. M.C. and D.M.M. were supported by National Science Foundation Grant IOS0920751 (to D.M.M.). T.S.-S. was supported by National Institutes of Health Grant DE017911 (to M.B.-F.). \n\nAuthor contributions: D.M.M. designed research; R.C., M.C., F.Y., and D.M.M. performed research; T.S.-S. and M.B.-F. contributed new reagents/analytic tools; R.C. and D.M.M. analyzed data; and D.M.M. wrote the paper. \n\nThe authors declare no conflict of interest. \n\nData deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. HQ248098\u2013HQ248103). \n\nThis article is a PNAS Direct Submission. \n\nThis article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1009304107/-/DCSupplemental.\n\nPublished - Cerny2010p11626P_Natl_Acad_Sci_Usa.pdf
Supplemental Material - pnas.201009304SI.pdf
", "abstract": "The appearance of jaws was a turning point in vertebrate evolution because it allowed primitive vertebrates to capture and process large, motile prey. The vertebrate jaw consists of separate dorsal and ventral skeletal elements connected by a joint. How this structure evolved from the unjointed gill bar of a jawless ancestor is an unresolved question in vertebrate evolution. To understand the developmental bases of this evolutionary transition, we examined the expression of 12 genes involved in vertebrate pharyngeal patterning in the modern jawless fish lamprey. We find nested expression of Dlx genes, as well as combinatorial expression of Msx, Hand and Gsc genes along the dorso-ventral (DV) axis of the lamprey pharynx, indicating gnathostome-type pharyngeal patterning evolved before the appearance of the jaw. In addition, we find that Bapx and Gdf5/6/7, key regulators of joint formation in gnathostomes, are not expressed in the lamprey first arch, whereas Barx, which is absent from the intermediate first arch in gnathostomes, marks this domain in lamprey. Taken together, these data support a new scenario for jaw evolution in which incorporation of Bapx and Gdf5/6/7 into a preexisting DV patterning program drove the evolution of the jaw by altering the identity of intermediate first-arch chondrocytes. We present this \"Pre-pattern/Cooption\" model as an alternative to current models linking the evolution of the jaw to the de novo appearance of sophisticated pharyngeal DV patterning.", "date": "2010-10-05", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "107", "number": "40", "publisher": "National Academy of Sciences", "pagerange": "17262-17267", "id_number": "CaltechAUTHORS:20101025-111202869", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20101025-111202869", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Academy of Sciences of the Czech Republic", "grant_number": "206/07/P257" }, { "agency": "Ministry of Education, Youth, and Sports (Czech Republic)", "grant_number": "0021620828" }, { "agency": "NSF", "grant_number": "IOS0920751" }, { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1073/pnas.1009304107", "pmcid": "PMC2951391", "primary_object": { "basename": "pnas.201009304SI.pdf", "url": "https://authors.library.caltech.edu/records/t8p1t-9k243/files/pnas.201009304SI.pdf" }, "related_objects": [ { "basename": "Cerny2010p11626P_Natl_Acad_Sci_Usa.pdf", "url": "https://authors.library.caltech.edu/records/t8p1t-9k243/files/Cerny2010p11626P_Natl_Acad_Sci_Usa.pdf" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Cerny, Robert; Cattell, Maria; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/61g2p-6kd65", "eprint_id": 20568, "eprint_status": "archive", "datestamp": "2023-08-19 03:49:16", "lastmod": "2023-10-20 23:12:13", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Strobl-Mazzulla-P-H", "name": { "family": "Strobl-Mazzulla", "given": "Pablo Hernan" }, "orcid": "0000-0003-0591-6168" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Histone Demethylase JmjD2A Regulates Neural Crest Specification", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2010 Elsevier. \n\nReceived 22 September 2009; revised 21 June 2010; accepted 13 August 2010. Published: September 13, 2010. Available online 14 September 2010. \n\nWe thank Dr. Scott Fraser for critical reading of the manuscript. This work was supported by USPHS grant HD037105.\n\nAccepted Version - nihms231559.pdf
Supplemental Material - mmc1.pdf
", "abstract": "The neural crest is a multipotent stem cell-like population that is induced during gastrulation, but only acquires its characteristic morphology, migratory ability, and gene expression profile after neurulation. This raises the intriguing possibility that precursors are actively maintained by epigenetic influences in a stem cell-like state. Accordingly, we report that dynamic histone modifications are critical for proper temporal control of neural crest gene expression in vivo. The histone demethylase, JumonjiD2A (JmjD2A/KDM4A), is expressed in the forming neural folds. Loss of JmjD2A function causes dramatic downregulation of neural crest specifier genes analyzed by multiplex NanoString and in situ hybridization. Importantly, in vivo chromatin immunoprecipitation reveals direct stage-specific interactions of JmjD2A with regulatory regions of neural crest genes, and associated temporal modifications in methylation states of lysine residues directly affected by JmjD2A activity. Our findings show that chromatin modifications directly control neural crest genes in vertebrate embryos via modulating histone methylation.", "date": "2010-09-14", "date_type": "published", "publication": "Developmental Cell", "volume": "19", "number": "3", "publisher": "Cell Press", "pagerange": "460-468", "id_number": "CaltechAUTHORS:20101027-150825589", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20101027-150825589", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1016/j.devcel.2010.08.009", "pmcid": "PMC2939072", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/61g2p-6kd65/files/mmc1.pdf" }, "related_objects": [ { "basename": "nihms231559.pdf", "url": "https://authors.library.caltech.edu/records/61g2p-6kd65/files/nihms231559.pdf" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Strobl-Mazzulla, Pablo Hernan; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sswb7-m6w37", "eprint_id": 20188, "eprint_status": "archive", "datestamp": "2023-08-22 00:41:47", "lastmod": "2023-10-20 22:18:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Fraser-G-J", "name": { "family": "Fraser", "given": "Gareth J." } }, { "id": "Cerny-R", "name": { "family": "Cerny", "given": "Robert" } }, { "id": "Soukup-V", "name": { "family": "Soukup", "given": "Vladimir" } }, { "id": "Streelman-J-T", "name": { "family": "Streelman", "given": "J. Todd" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The odontode explosion: The origin of tooth-like structures in vertebrates", "ispublished": "pub", "full_text_status": "public", "keywords": "dentition; gene network; neural crest; odontode; taste bud", "note": "\u00a9 2010 Wiley Periodicals, Inc. \n\nArticle first published online: 17 Aug. 2010. \n\nWe thank Brandon Milholland for his work on the genes expressed during cephalic lateral line development in Malawi cichlids. We also thank Moya Smith, Zerina Johanson, Ivan Horacek, Martin Kralovic and four anonymous reviewers for their comments on previous versions of the manuscript. This work was supported by grants awarded to J. T. S. (NIH-R01DE019637) and R. C. (MSMT-0021620828; GACR-206/09/10007).\n\nAccepted Version - nihms266189.pdf
", "abstract": "Essentially we show recent data to shed new light on the thorny controversy of how teeth arose in evolution. Essentially we show (a) how teeth can form equally from any epithelium, be it endoderm, ectoderm or a combination of the two and (b) that the gene expression programs of oral versus pharyngeal teeth are remarkably similar. Classic theories suggest that (i) skin denticles evolved first and odontode-inductive surface ectoderm merged inside the oral cavity to form teeth (the 'outside-in' hypothesis) or that (ii) patterned odontodes evolved first from endoderm deep inside the pharyngeal cavity (the 'inside-out' hypothesis). We propose a new perspective that views odontodes as structures sharing a deep molecular homology, united by sets of co-expressed genes defining a competent thickened epithelium and a collaborative neural crest-derived ectomesenchyme. Simply put, odontodes develop 'inside and out', wherever and whenever these co-expressed gene sets signal to one another. Our perspective complements the classic theories and highlights an agenda for specific experimental manipulations in model and non-model organisms.", "date": "2010-09", "date_type": "published", "publication": "Bioessays", "volume": "32", "number": "9", "publisher": "Wiley-Blackwell", "pagerange": "808-817", "id_number": "CaltechAUTHORS:20100928-105119416", "issn": "0265-9247", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20100928-105119416", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE019637" }, { "agency": "Ministerstvo \u0160kolstv\u00ed, Ml\u00e1deze a Telov\u00fdchovy (MSMT)", "grant_number": "0021620828" }, { "agency": "Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky (GACR)", "grant_number": "206/09/10007" } ] }, "doi": "10.1002/bies.200900151", "pmcid": "PMC3034446", "primary_object": { "basename": "nihms266189.pdf", "url": "https://authors.library.caltech.edu/records/sswb7-m6w37/files/nihms266189.pdf" }, "resource_type": "article", "pub_year": "2010", "author_list": "Fraser, Gareth J.; Cerny, Robert; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xd8rq-rhk93", "eprint_id": 18333, "eprint_status": "archive", "datestamp": "2023-08-21 23:45:40", "lastmod": "2023-10-20 16:07:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Pax2 and Pea3 synergize to activate a novel regulatory enhancer for spalt4 in the developing ear", "ispublished": "pub", "full_text_status": "public", "keywords": "Chick; Sall4; Otic induction; Inner ear; FGF8", "note": "\u00a9 2009 Elsevier Inc. \n\nReceived 1 October 2009; revised 30 October 2009; accepted 2 November 2009. Available online 10 November 2009. \n\nThis article was accepted in 2009 to celebrate the 50th anniversary of Developmental Biology. \n\nWe thank Dr. Tatjana Sauka-Spengler for her expert help and\nadvice through all aspects of this project. We would like to thank Dr.\nHisato Kondoh for providing the pTK vectors. We also want to thank\nDr. Tatiana Hochgreb for helping with the confocal microscope. This\nwork was supported by USPHS grants DE16459 and HD037105.\n\nAccepted Version - nihms208499.pdf
", "abstract": "The transcription factor spalt4 is a key early-response gene in otic placode induction. Here, we characterize the cis-regulatory regions of spalt4 responsible for activation of its expression in the developing otic placode and report the isolation of a novel core enhancer. Identification and mutational analysis of putative transcription factor binding sites reveal that Pea3, a downstream effector of FGF signaling, and Pax2 directly activate spalt4 during ear development. Morpholino-mediated knock-down of each factor reduces or eliminates reporter expression. In contrast, combined over-expression of Pea3 and Pax2 drives ectopic reporter expression, suggesting that they function synergistically. These studies expand the gene regulatory network underlying early otic development by identifying direct inputs that mediate spalt4 expression.", "date": "2010-04-15", "date_type": "published", "publication": "Developmental Biology", "volume": "340", "number": "2", "publisher": "Elsevier", "pagerange": "222-231", "id_number": "CaltechAUTHORS:20100518-093533680", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20100518-093533680", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1016/j.ydbio.2009.11.004", "pmcid": "PMC2892740", "primary_object": { "basename": "nihms208499.pdf", "url": "https://authors.library.caltech.edu/records/xd8rq-rhk93/files/nihms208499.pdf" }, "resource_type": "article", "pub_year": "2010", "author_list": "Barembaum, Meyer and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nqt09-hdz08", "eprint_id": 17868, "eprint_status": "archive", "datestamp": "2023-08-19 01:42:12", "lastmod": "2023-10-20 15:21:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Betancur-P", "name": { "family": "Betancur", "given": "Paola" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2010 by the National Academy of Sciences. \n\nEdited by Eric H. Davidson, California Institute of Technology, Pasadena, CA, and approved December 8, 2009 (received for review June 16, 2009). \n\nWe thank Dr. S. Fraser for critical reading of the manuscript, M. Jones for excellent technical assistance, Drs. H. Kondoh for ptk-EGFP reporter construct, Y.-C. Cheng for full-length Sox9 and Sox10 constructs, M. Dvorak for gift of cMyb antibody, M. Wegner for Sox9 antibody, P. Strobl for help with H3K36me3 ChIP, G. Hernandez and D. Meulemans Medeiros for helpful discussions, and M. Barembaum and M. Morales-Del Real for help with in situs. This work was supported by a California Institute of Regenerative Medicine Fellowship (to T.S.S.) and Grants NS36585 and P01-HD037105 (to M.B.-F.). \n\nAuthor contributions: P.B., M.B.-F., and T.S.-S. designed research; P.B. and T.S.-S. performed research; T.S.-S. contributed new reagents/analytic tools; P.B. and T.S.-S. analyzed data; and P.B., M.B.-F., and T.S.-S. wrote the paper.\n\nThe authors declare no conflict of interest. \n\nThis article is a PNAS Direct Submission. \n\nThis article contains supporting information online at www.pnas.org/cgi/content/full/0906596107/DCSupplemental.\n\nPublished - Betancur2010p7283P_Natl_Acad_Sci_Usa.pdf
Supplemental Material - pnas.200906596SI.pdf
", "abstract": "The neural crest is a multipotent, stem cell-like population that migrates extensively in the embryo and forms a wide array of derivatives, ranging from neurons to melanocytes and cartilage. Analyses of the gene regulatory network driving neural crest development revealed Sox10 as one of the earliest neural crest-specifying genes, cell-autonomously driving delamination and directly regulating numerous downstream effectors and differentiation gene batteries. In search of direct inputs to the neural crest specifier module, we dissected the chick Sox10 genomic region and isolated two downstream regulatory regions with distinct spatiotemporal activity. A unique element, Sox10E2 represents the earliest-acting neural crest cis-regulatory element, critical for initiating Sox10 expression in newly formed cranial, but not vagal and trunk neural crest. A second element, Sox10E1, acts in later migrating vagal and trunk crest cells. Deep characterization of Sox10E2 reveals Sox9, Ets1, and cMyb as direct inputs mediating enhancer activity. ChIP, DNA-pull down, and gel-shift assays demonstrate their direct binding to the Sox10E2 enhancer in vivo, whereas mutation of their corresponding binding sites, or inactivation of the three upstream regulators, abolishes both reporter and endogenous Sox10 expression. Using cis-regulatory analysis as a tool, our study makes critical connections within the neural crest gene regulatory network, thus being unique in establishing a direct link of upstream effectors to a key neural crest specifier.", "date": "2010-02-23", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "107", "number": "8", "publisher": "National Academy of Sciences", "pagerange": "3570-3575", "id_number": "CaltechAUTHORS:20100406-105200553", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20100406-105200553", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California Institute for Regenerative Medicine (CIRM)" }, { "agency": "NIH", "grant_number": "P01-HD037105" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1073/pnas.0906596107", "pmcid": "PMC2840498", "primary_object": { "basename": "pnas.200906596SI.pdf", "url": "https://authors.library.caltech.edu/records/nqt09-hdz08/files/pnas.200906596SI.pdf" }, "related_objects": [ { "basename": "Betancur2010p7283P_Natl_Acad_Sci_Usa.pdf", "url": "https://authors.library.caltech.edu/records/nqt09-hdz08/files/Betancur2010p7283P_Natl_Acad_Sci_Usa.pdf" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Betancur, Paola; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/04d4n-6kw96", "eprint_id": 23184, "eprint_status": "archive", "datestamp": "2023-08-21 23:21:08", "lastmod": "2023-10-23 18:01:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Castillo-Hozana-A", "name": { "family": "Castillo", "given": "Hozana A." } }, { "id": "Cravo-Roberta-M", "name": { "family": "Cravo", "given": "Roberta M." } }, { "id": "Azambuja-Ana-P", "name": { "family": "Azambuja", "given": "Ana P." } }, { "id": "Sim\u00f5es-Costa-Marcos-S", "name": { "family": "Sim\u00f5es-Costa", "given": "Marcos S." }, "orcid": "0000-0003-1452-7068" }, { "id": "Sura-Trueba-Sylvia", "name": { "family": "Sura-Trueba", "given": "Sylvia" } }, { "id": "Gonzalez-Jose", "name": { "family": "Gonzalez", "given": "Jose" } }, { "id": "Slonimsky-Esfir", "name": { "family": "Slonimsky", "given": "Esfir" } }, { "id": "Almeida-Karla", "name": { "family": "Almeida", "given": "Karla" } }, { "id": "Abreu-Jos\u00e9-G", "name": { "family": "Abreu", "given": "Jos\u00e9 G." } }, { "id": "Afonso-de-Almeida-Marcio-A", "name": { "family": "Afonso de Almeida", "given": "Marcio A." } }, { "id": "Sobreira-Tiago-P", "name": { "family": "Sobreira", "given": "Tiago P." } }, { "id": "Pires-de-Oliveira-Saulo-H", "name": { "family": "Pires de Oliveira", "given": "Saulo H." } }, { "id": "Lopes-de-Oliveira-Paulo-S", "name": { "family": "Lopes de Oliveira", "given": "Paulo S." } }, { "id": "Signore-Iskra-A", "name": { "family": "Signore", "given": "Iskra A." } }, { "id": "Colombo-Alicia", "name": { "family": "Colombo", "given": "Alicia" } }, { "id": "Concha-Miguel-L", "name": { "family": "Concha", "given": "Miguel L." } }, { "id": "Sauka-Spengler-Tatjana", "name": { "family": "Spengler", "given": "Tatjana S." }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Nobrega-Marcelo", "name": { "family": "Nobrega", "given": "Marcelo" } }, { "id": "Rosenthal-Nadia", "name": { "family": "Rosenthal", "given": "Nadia" } }, { "id": "Xavier-Neto-Jos\u00e9", "name": { "family": "Xavier-Neto", "given": "Jos\u00e9" } } ] }, "title": "Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer", "ispublished": "pub", "full_text_status": "public", "keywords": "Retinoic acid; Spinal cord; Roof plate; Commissural interneurons; Proprioception; Paired fin loss; Mouse; Chicken; Xenopus; Zebrafish; Lamprey; Medaka", "note": "\u00a9 2010 The Company of Biologists Ltd. \n\nAccepted 4 December 2009. \n\nWe are indebted to Ursula Dr\u00e4ger, Peter McCaffery and Marcus Vinicius Baldo for comments and suggestions; to Richard Behringer and Wellington Cardoso for comments on the manuscript; to Masanori Uchikawa and Jane Johnson for reagents; and to the Faculty of Medicine of the University of S\u00e3o Paulo for access to its high-performance computing cluster. This work was supported by grants from FAPESP (02/11340-2; 04/11569-5; 04/15704-4; 05/60637-6; 06/50843-0; 06/61317-8), CNPq 305260/2007-3 and by a Development Travelling Fellowship from The Company of Biologists.\n\nPublished - Castillo2010p7002Development.pdf
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", "abstract": "Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dl1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dl1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception.", "date": "2010-02-01", "date_type": "published", "publication": "Development", "volume": "137", "number": "3", "publisher": "Company of Biologists", "pagerange": "507-518", "id_number": "CaltechAUTHORS:20110330-160058995", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110330-160058995", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "02/11340-2" }, { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "04/11569-5" }, { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "04/15704-4" }, { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "05/60637-6" }, { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "06/50843-0" }, { "agency": "Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)", "grant_number": "06/61317-8" }, { "agency": "Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)", "grant_number": "305260/2007-3" }, { "agency": "Company of Biologists" } ] }, "doi": "10.1242/dev.043257", "pmcid": "PMC4074295", "primary_object": { "basename": "043257FigS1.jpg", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/043257FigS1.jpg" }, "related_objects": [ { "basename": "043257FigS2.jpg", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/043257FigS2.jpg" }, { "basename": "043257FigS3.jpg", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/043257FigS3.jpg" }, { "basename": "043257FigS4.jpg", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/043257FigS4.jpg" }, { "basename": "043257FigS5.jpg", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/043257FigS5.jpg" }, { "basename": "Castillo2010p7002Development.pdf", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/Castillo2010p7002Development.pdf" }, { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/medium.png" }, { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/04d4n-6kw96/files/small.png" } ], "resource_type": "article", "pub_year": "2010", "author_list": "Castillo, Hozana A.; Cravo, Roberta M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zjxts-myw11", "eprint_id": 16918, "eprint_status": "archive", "datestamp": "2023-08-21 22:54:54", "lastmod": "2023-10-19 22:39:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Shiau-Celia-E", "name": { "family": "Shiau", "given": "Celia E." }, "orcid": "0000-0002-9347-9158" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "N-cadherin acts in concert with Slit1-Robo2 signaling in regulating aggregation of placode-derived cranial sensory neurons", "ispublished": "pub", "full_text_status": "public", "keywords": "Cadherins; Slits/Robos; Placode; Neural crest; Gangliogenesis; Trigeminal; Chick", "note": "\u00a9 2009 Company of Biologists Ltd. \n\nAccepted October 12, 2009; first published online November 23, 2009. \n\nWe thank Drs J. Raper and Z. Kaprielian for the CMV-Robo2FL-myc plasmid; Dr M. Takeichi for the pCMV-cN/FLAG-pA plasmid; members of M.B.-F. laboratory for discussions and technical support; and Dr M. Barembaum for critical comments on the manuscript. This work was supported by US National Institutes of Health (NIH) National Research Service Award 5T32 GM07616 to C.E.S. and NIH grant DE16459 to M.B.-F. Deposited in PMC for release after 12 months.\n\nPublished - Shiau2009p6516Development.pdf
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", "abstract": "Vertebrate cranial sensory ganglia have a dual origin from the neural crest and ectodermal placodes. In the largest of these, the trigeminal ganglion, Slit1-Robo2 signaling is essential for proper ganglion assembly. Here, we demonstrate a crucial role for the cell adhesion molecule N-cadherin and its interaction with Slit1-Robo2 during gangliogenesis in vivo. A common feature of chick trigeminal and epibranchial ganglia is the expression of N-cadherin and Robo2 on placodal neurons and Slit1 on neural crest cells. Interestingly, N-cadherin localizes to intercellular adherens junctions between placodal neurons during ganglion assembly. Depletion of N-cadherin causes loss of proper ganglion coalescence, similar to that observed after loss of Robo2, suggesting that the two pathways might intersect. Consistent with this possibility, blocking or augmenting Slit-Robo signaling modulates N-cadherin protein expression on the placodal cell surface concomitant with alteration in placodal adhesion. Lack of an apparent change in total N-cadherin mRNA or protein levels suggests post-translational regulation. Co-expression of N-cadherin with dominant-negative Robo abrogates the Robo2 loss-of-function phenotype of dispersed ganglia, whereas loss of N-cadherin reverses the aberrant aggregation induced by increased Slit-Robo expression. Our study suggests a novel mechanism whereby N-cadherin acts in concert with Slit-Robo signaling in mediating the placodal cell adhesion required for proper gangliogenesis.", "date": "2009-12-15", "date_type": "published", "publication": "Development", "volume": "136", "number": "24", "publisher": "Company of Biologists", "pagerange": "4155-4164", "id_number": "CaltechAUTHORS:20091209-093920989", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20091209-093920989", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5T32 GM07616" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1242/dev.034355", "pmcid": "PMC2781051", "primary_object": { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/medium.png" }, "related_objects": [ { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/small.png" }, { "basename": "034355-FigS1.jpg", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/034355-FigS1.jpg" }, { "basename": "034355-FigS2.jpg", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/034355-FigS2.jpg" }, { "basename": "034355-FigS3.jpg", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/034355-FigS3.jpg" }, { "basename": "034355-FigS4.jpg", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/034355-FigS4.jpg" }, { "basename": "034355-FigS5.jpg", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/034355-FigS5.jpg" }, { "basename": "Shiau2009p6516Development.pdf", "url": "https://authors.library.caltech.edu/records/zjxts-myw11/files/Shiau2009p6516Development.pdf" } ], "resource_type": "article", "pub_year": "2009", "author_list": "Shiau, Celia E. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nxhps-1zv25", "eprint_id": 17020, "eprint_status": "archive", "datestamp": "2023-08-21 22:55:10", "lastmod": "2023-10-19 22:44:08", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Semaphorin3A/neuropilin-1 signaling acts as a molecular switch regulating neural crest migration during cornea development", "ispublished": "pub", "full_text_status": "public", "keywords": "Semaphorin3A; Neuropilin-1; Neural crest; Cornea; Lens", "note": "\u00a9 2009 Elsevier. \n\nReceived 2 April 2009; revised 11 September 2009; accepted 6 October 2009; available online 13 October 2009. \n\nThe authors would like to thank Jonathan A. Raper for the fulllength Npn-1 cDNA and Anitha Rao for technical assistance. This work was supported in part by NIH grants EY018050 (P.Y.L.) and DE016459 (M.B.-F.).\n\nAppendix A: Supplementary data associated with this article can be found, in the online version, at doi:10.1016/j.ydbio.2009.10.008.\n\nAccepted Version - nihms161799.pdf
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", "abstract": "Cranial neural crest cells migrate into the periocular region and later contribute to various ocular tissues including the cornea, ciliary body and iris. After reaching the eye, they initially pause before migrating over the lens to form the cornea. Interestingly, removal of the lens leads to premature invasion and abnormal differentiation of the cornea. In exploring the molecular mechanisms underlying this effect, we find that semaphorin3A (Sema3A) is expressed in the lens placode and epithelium continuously throughout eye development. Interestingly, neuropilin-1 (Npn-1) is expressed by periocular neural crest but down-regulated, in a manner independent of the lens, by the subpopulation that migrates into the eye and gives rise to the cornea endothelium and stroma. In contrast, Npn-1 expressing neural crest cells remain in the periocular region and contribute to the anterior uvea and ocular blood vessels. Introduction of a peptide that inhibits Sema3A/Npn-1 signaling results in premature entry of neural crest cells over the lens that phenocopies lens ablation. Furthermore, Sema3A inhibits periocular neural crest migration in vitro. Taken together, our data reveal a novel and essential role of Sema3A/Npn-1 signaling in coordinating periocular neural crest migration that is vital for proper ocular development.", "date": "2009-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "336", "number": "2", "publisher": "Elsevier", "pagerange": "257-265", "id_number": "CaltechAUTHORS:20091222-122306746", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20091222-122306746", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "EY018050" }, { "agency": "NIH", "grant_number": "DE016459" } ] }, "doi": "10.1016/j.ydbio.2009.10.008", "pmcid": "PMC2800376", "primary_object": { "basename": "Fig._S1.jpg", "url": "https://authors.library.caltech.edu/records/nxhps-1zv25/files/Fig._S1.jpg" }, "related_objects": [ { "basename": "Fig._S2.jpg", "url": "https://authors.library.caltech.edu/records/nxhps-1zv25/files/Fig._S2.jpg" }, { "basename": "nihms161799.pdf", "url": "https://authors.library.caltech.edu/records/nxhps-1zv25/files/nihms161799.pdf" } ], "resource_type": "article", "pub_year": "2009", "author_list": "Lwigale, Peter Y. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0skme-gj409", "eprint_id": 16633, "eprint_status": "archive", "datestamp": "2023-08-21 22:39:45", "lastmod": "2023-10-19 22:26:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nie-Shuyi", "name": { "family": "Nie", "given": "Shuyi" } }, { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Myosin-X is critical for migratory ability of Xenopus cranial neural crest cells", "ispublished": "pub", "full_text_status": "public", "keywords": "Myosin-X; Cranial neural crest; Migration; Adhesion", "note": "\u00a9 2009 Published by Elsevier Inc. \n\nReceived 11 February 2009; revised 18 August 2009; accepted 18 August 2009. Available online 25 August 2009. \n\nWe thank Dr. William M. Bement for providing us full-length Xenopus Myo10 constructs, Dr. Dominique Alfandari for providing protocols for grafting experiment, Dr. Chenbei Chang for providing Wnt3a and Noggin constructs, and Dr. Ken Cho for providing microarray analysis tool. This work was supported by NS36585 and HD037105 to MBF.\n\nAccepted Version - nihms-568059.pdf
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", "abstract": "The neural crest is a highly migratory cell population, unique to vertebrates, that forms much of the craniofacial skeleton and peripheral nervous system. In exploring the cell biological basis underlying this behavior, we have identified an unconventional myosin, myosin-X (Myo10) that is required for neural crest migration. Myo10 is highly expressed in both premigratory and migrating cranial neural crest (CNC) cells in Xenopus embryos. Disrupting Myo10 expression using antisense morpholino oligonucleotides leads to impaired neural crest migration and subsequent cartilage formation, but only a slight delay in induction. In vivo grafting experiments reveal that Myo10-depleted CNC cells migrate a shorter distance and fail to segregate into distinct migratory streams. Finally, in vitro cultures and cell dissociation\u2013reaggregation assays suggest that Myo10 may be critical for cell protrusion and cell\u2013cell adhesion. These results demonstrate an essential role for Myo10 in normal cranial neural crest migration and suggest a link to cell\u2013cell interactions and formation of processes.", "date": "2009-11-01", "date_type": "published", "publication": "Developmental Biology", "volume": "335", "number": "1", "publisher": "Elsevier", "pagerange": "132-142", "id_number": "CaltechAUTHORS:20091110-083154002", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20091110-083154002", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "HD037105" } ] }, "doi": "10.1016/j.ydbio.2009.08.018", "pmcid": "PMC3991693", "primary_object": { "basename": "Nie2009p6278Dev_Biol_sup1.mov", "url": "https://authors.library.caltech.edu/records/0skme-gj409/files/Nie2009p6278Dev_Biol_sup1.mov" }, "related_objects": [ { "basename": "Nie2009p6278Dev_Biol_sup2.mov", "url": "https://authors.library.caltech.edu/records/0skme-gj409/files/Nie2009p6278Dev_Biol_sup2.mov" }, { "basename": "Nie2009p6278Dev_Biol_sup3.mov", "url": "https://authors.library.caltech.edu/records/0skme-gj409/files/Nie2009p6278Dev_Biol_sup3.mov" }, { "basename": "nihms-568059.pdf", "url": "https://authors.library.caltech.edu/records/0skme-gj409/files/nihms-568059.pdf" } ], "resource_type": "article", "pub_year": "2009", "author_list": "Nie, Shuyi; Kee, Yun; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/52q8n-c5903", "eprint_id": 17091, "eprint_status": "archive", "datestamp": "2023-08-19 00:13:22", "lastmod": "2023-10-19 22:48:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "de-Bellard-M-E", "name": { "family": "de Bellard", "given": "M. E." } }, { "id": "Waldheim-C", "name": { "family": "Waldheim", "given": "C." } }, { "id": "Kim-L", "name": { "family": "Kim", "given": "L." } }, { "id": "Walker-J", "name": { "family": "Walker", "given": "J." } }, { "id": "Ollison-L", "name": { "family": "Ollison", "given": "L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Effects of neurotrophins on neural crest and Schwann cell migration", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2009 Wiley-Liss, Inc. \n\nPoster #: P-343 \n\nPublished Online: 24 Aug 2009. \n\nThis work was supported by a grant from NIH 5 R01 HD15527-15, an NIH SCORE grant 2-SO6-GM048680-12A1 and a NMSS fellowship to MEdB FA1383-A-1.", "abstract": "The neural crest is a migratory population of cells that gives rise to a\nwide range of cell types in the peripheral nervous system of vertebrate\nembryos. It has been shown that neural crest cells migrate along very\nspecific pathways throughout the embryo. The reason for such specificity\nis not fully known. During the last years, some known axon pathfinding\nrepellants (ephrinB2, SemaIIIa, Slit2, etc) have been shown to\nrepel neural crest cells as well during their migration through the\nsomites. However, we know very little about the migratory clues that\nguide the neural crest for the rest of their path. The goal of this study\nwas to find which other molecules are capable of guiding the neural\ncrest. For this purpose we had set out to screen a group of neurotrophic\nfactors that are expressed at the same time that the crest is migrating\nthrough the embryo. Our aim was to look at the effect of neurotrophins\non neural crest migration and Schwann cell precursors. Experiments\nby live imaging in special chambers suggest that: a) neural crest cells\nare attracted to glia derived neurotrophic factor (GDNF) and Heregulinb1;\nand b) that Schwann cell precursors increase their speed in\nthe presence of GDNF, NGF, Heregulinb1 and Macrophage inhibitory\nfactor (MIF). These preliminary data suggests that neural crest and\nSchwann cells use a variety of neurotrophic factors as guiding clues\nduring their extensive migration in the embryo.", "date": "2009-10", "date_type": "published", "publication": "Glia", "volume": "57", "number": "13", "publisher": "Wiley", "pagerange": "S108-S109", "id_number": "CaltechAUTHORS:20100107-110905611", "issn": "0894-1491", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20100107-110905611", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5 R01 HD15527-15" }, { "agency": "NIH", "grant_number": "2-SO6-GM048680-12A1" }, { "agency": "National Multiple Sclerosis Society", "grant_number": "MEdB FA1383-A-1" } ] }, "doi": "10.1002/glia.20915", "resource_type": "article", "pub_year": "2009", "author_list": "de Bellard, M. E.; Waldheim, C.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/h9n6f-8kj41", "eprint_id": 18722, "eprint_status": "archive", "datestamp": "2023-08-18 23:57:48", "lastmod": "2023-10-20 16:42:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Jiang-Xiaohua", "name": { "family": "Jiang", "given": "Xiaohua" } }, { "id": "Gwye-Y", "name": { "family": "Gwye", "given": "Ynnez" } }, { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Lutzko-C", "name": { "family": "Lutzko", "given": "Carolyn" } }, { "id": "Lawlor-E-R", "name": { "family": "Lawlor", "given": "Elizabeth R." } } ] }, "title": "Isolation and Characterization of Neural Crest Stem Cells Derived From In Vitro\u2013Differentiated Human Embryonic Stem Cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Mary Ann Liebert, Inc. \n\nPublished in Volume: 18 Issue 7: September 10, 2009. Online Ahead of Print: June 11, 2009. Online Ahead of Editing: December 19, 2008. \n\nThe authors gratefully acknowledge Dinithi Seandheera, Xingcao Wang, Lora Barsky, and the Saban Research Institute Stem Cell and FACS Cores at Childrens Hospital Los Angeles for their invaluable assistance. Support for this work was provided by CIRM SEED grant (RS1-00249) and by a Stop Cancer Foundation Career Development Award (E.R.L.). X.J. was supported by a CIRM Post-docotral Scholarship through Training Grant T2-00005. S.J.M. was supported by an NH&MRC CJ Martin fellowship. Additional financial support from the TJ Martell and My Brother Joey Foundations is also gratefully acknowledged. This work was presented in part at the 6th Annual Meeting of the ISSCR, Phildelphia, PA, June 2008.\n\nPublished - scd.2008.0362.pdf
", "abstract": "The neural crest is a transient structure of vertebrate embryos that initially generates neural crest stem cells (NCSCs) which then migrate throughout the body to produce a diverse array of mature tissue types. Due to the rarity of adult NCSCs as well as ethical and technical issues surrounding isolation of early embryonic tissues, biologic studies of human NCSCs are extremely challenging. Thus, much of what is known about human neural crest development has been inferred from model organisms. In this study, we report that functional NCSCs can be rapidly generated and isolated from in vitro\u2014differentiated human embryonic stem cells (hESCs). Using the stromal-derived inducing activity (SDIA) of PA6 fibroblast co-culture we have induced hESCs to differentiate into neural crest. Within 1 week, migrating cells that express the early neural crest markers p75 and HNK1 as well as numerous other genes associated with neural crest induction such as SNAIL, SLUG, and SOX10 are detectable. Fluorescence-activated cell sorting (FACS)-based isolation of the p75-positive population enriches for cells with genetic, phenotypic, and functional characteristics of NCSCs. These p75-enriched cells readily form neurospheres in suspension culture, self-renew to form secondary spheres, and give rise under differentiation conditions to multiple neural crest lineages including peripheral nerves, glial, and myofibroblastic cells. Importantly, these cells differentiate into neural crest derivatives when transplanted into developing chick embryos in vivo. Thus, this SDIA protocol can be used to successfully and efficiently isolate early human NCSCs from hESCs in vitro. This renewable source of NCSCs provides an invaluable source of cells for studies of both normal and disordered human neural crest development.", "date": "2009-09", "date_type": "published", "publication": "Stem Cells and Development", "volume": "18", "number": "7", "publisher": "Mary Ann Liebert", "pagerange": "1059-1070", "id_number": "CaltechAUTHORS:20100617-133544868", "issn": "1547-3287", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20100617-133544868", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "RS1-00249" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "T2-00005" }, { "agency": "Stop Cancer Foundation" }, { "agency": "National Health and Medical Research Council (NHMRC)" }, { "agency": "T. J. Martell Foundation" }, { "agency": "My Brother Joey Foundation" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1089/scd.2008.0362", "pmcid": "PMC4606969", "primary_object": { "basename": "scd.2008.0362.pdf", "url": "https://authors.library.caltech.edu/records/h9n6f-8kj41/files/scd.2008.0362.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "Jiang, Xiaohua; Gwye, Ynnez; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fzjbp-erd25", "eprint_id": 15331, "eprint_status": "archive", "datestamp": "2023-08-21 22:04:50", "lastmod": "2023-10-18 21:40:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular and tissue interactions governing induction of cranial ectodermal placodes", "ispublished": "pub", "full_text_status": "public", "keywords": "Placode induction; Epibranchial; Trigeminal; Shh; Wnt; TGF beta; Platelet derived growth factor", "note": "Copyright \u00a9 2009 Elsevier. \n\nReceived 28 January 2009; revised 27 May 2009; accepted 28 May 2009. Available online 2 June 2009. \n\nWe would like to thank Drs. Sonja McKeown and Sujata Bhattacharyya for critical reading of the manuscript. This work was supported by NIH R01DE16459.\n\nAccepted Version - nihms127698.pdf
", "abstract": "Whereas neural crest cells are the source of the peripheral nervous system in the trunk of vertebrates, the \"ectodermal placodes,\" together with neural crest, form the peripheral nervous system of the head. Cranial ectodermal placodes are thickenings in the ectoderm that subsequently ingress or invaginate to make important contributions to cranial ganglia, including epibranchial and trigeminal ganglia, and sensory structures, the ear, nose, lens, and adenohypophysis. Recent studies have uncovered a number of molecular signals mediating induction and differentiation of placodal cells. Here, we described recent advances in understanding the tissue interactions and signals underlying induction and neurogenesis of placodes, with emphasis on the trigeminal and epibranchial. Important roles of Fibroblast Growth Factors, Platelet Derived Growth Factors, Sonic Hedgehog, TGF\u03b2 superfamily members, and Wnts are discussed.", "date": "2009-08-15", "date_type": "published", "publication": "Developmental Biology", "volume": "332", "number": "2", "publisher": "Elsevier", "pagerange": "189-195", "id_number": "CaltechAUTHORS:20090826-112854174", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090826-112854174", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01DE16459" } ] }, "doi": "10.1016/j.ydbio.2009.05.572", "pmcid": "PMC2747488", "primary_object": { "basename": "nihms127698.pdf", "url": "https://authors.library.caltech.edu/records/fzjbp-erd25/files/nihms127698.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "McCabe, Kathryn L. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fhyy9-rcj53", "eprint_id": 14669, "eprint_status": "archive", "datestamp": "2023-08-21 21:38:44", "lastmod": "2023-10-18 18:41:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ezin-A-M", "name": { "family": "Ezin", "given": "Akouavi M." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Fate map and morphogenesis of presumptive neural crest and dorsal neural tube", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Dorsal neural tube; Neural plate border; Neural plate; Cell movements; Gastrulation; Neurulation; Chick; Convergent extension; Reorientation; Deep motility; Morphogenesis; Fate map", "note": "\u00a9 2009 Published by Elsevier Inc. \n\nReceived 30 March 2008; revised 9 February 2009; accepted 12 March 2009. Available online 28 March 2009. \n\nWe thank the members of the Bronner-Fraser and Fraser labs. We first and foremost thank Jack Sechrist for training and the innumerable hours he spent helping with analysis of histological sections and with correlating injection spots on time-lapse sequences with dye labels on cryosections. Special thanks also to Tatajana Demyanenko for help with sectioning embryos and Christopher Waters for assistance in the Beckman Imaging Center. Thanks also to Matthew Jones for help with sectioning embryos. This work was funded by NIH grant NS36585 and NRSA 5F32HL078141.\n\nAccepted Version - nihms121352.pdf
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", "abstract": "In contrast to the classical assumption that neural crest cells are induced in chick as the neural folds elevate, recent data suggest that they are already specified during gastrulation. This prompted us to map the origin of the neural crest and dorsal neural tube in the early avian embryo. Using a combination of focal dye injections and time-lapse imaging, we find that neural crest and dorsal neural tube precursors are present in a broad, crescent-shaped region of the gastrula. Surprisingly, static fate maps together with dynamic confocal imaging reveal that the neural plate border is considerably broader and extends more caudally than expected. Interestingly, we find that the position of the presumptive neural crest broadly correlates with the BMP4 expression domain from gastrula to neurula stages. Some degree of rostrocaudal patterning, albeit incomplete, is already evident in the gastrula. Time-lapse imaging studies show that the neural crest and dorsal neural tube precursors undergo choreographed movements that follow a spatiotemporal progression and include convergence and extension, reorientation, cell intermixing, and motility deep within the embryo. Through these rearrangement and reorganization movements, the neural crest and dorsal neural tube precursors become regionally segregated, coming to occupy predictable rostrocaudal positions along the embryonic axis. This regionalization occurs progressively and appears to be complete in the neurula by stage 7 at levels rostral to Hensen's node.", "date": "2009-06-15", "date_type": "published", "publication": "Developmental Biology", "volume": "330", "number": "2", "publisher": "Elsevier", "pagerange": "221-236", "id_number": "CaltechAUTHORS:20090727-094307609", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090727-094307609", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NRSA 5F32HL078141" } ] }, "doi": "10.1016/j.ydbio.2009.03.018", "pmcid": "PMC2717095", "primary_object": { "basename": "video2.mov", "url": "https://authors.library.caltech.edu/records/fhyy9-rcj53/files/video2.mov" }, "related_objects": [ { "basename": "video3.mov", "url": "https://authors.library.caltech.edu/records/fhyy9-rcj53/files/video3.mov" }, { "basename": "nihms121352.pdf", "url": "https://authors.library.caltech.edu/records/fhyy9-rcj53/files/nihms121352.pdf" }, { "basename": "video1.mov", "url": "https://authors.library.caltech.edu/records/fhyy9-rcj53/files/video1.mov" } ], "resource_type": "article", "pub_year": "2009", "author_list": "Ezin, Akouavi M.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xdfpd-hbc04", "eprint_id": 14540, "eprint_status": "archive", "datestamp": "2023-08-20 01:57:50", "lastmod": "2023-10-18 18:05:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Acloque-H", "name": { "family": "Acloque", "given": "Herv\u00e9" } }, { "id": "Adams-M-S", "name": { "family": "Adams", "given": "Meghan S." } }, { "id": "Fishwick-K-J", "name": { "family": "Fishwick", "given": "Katherine" } }, { "id": "Nieto-M-A", "name": { "family": "Nieto", "given": "M. Angela" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 American Society for Clinical Investigation. \n\nPublished June 1, 2009. \n\nThe work in our laboratories is supported by grants from the Spanish Ministry of Science and Innovation (grants BFU2005-05772, BFU2008-01042, NAN2004-09230-C04-04, CONSOLIDER-INGENIO 2010 CSD2007-00017 and CSD2007-00023) and the Generalitat Valenciana (Prometeo/2008/049) to M.A. Nieto; and by NIH grants R01 DE017911 and P50HG004071 to M. Bronner-Fraser. H. Acloque was supported by the I3P Programme (European Social Fund/MEC) and M.S. Adams by the Morgan fellowship from the Gordon and Betty Moore Foundation.\n\nPublished - Acloque2009p4429Journal_of_Clinical_Investigation.pdf
", "abstract": "The events that convert adherent epithelial cells into individual migratory cells that can invade the extracellular matrix are known collectively as epithelial-mesenchymal transition (EMT). Throughout evolution, the capacity of cells to switch between these two cellular states has been fundamental in the generation of complex body patterns. Here, we review the EMT events that build the embryo and further discuss two prototypical processes governed by EMT in amniotes: gastrulation and neural crest formation. Cells undergo EMT to migrate and colonize distant territories. Not surprisingly, this is also the mechanism used by cancer cells to disperse throughout the body.", "date": "2009-06-01", "date_type": "published", "publication": "Journal of Clinical Investigation", "volume": "119", "number": "6", "publisher": "American Society for Clinical Investigation", "pagerange": "1438-1449", "id_number": "CaltechAUTHORS:20090709-100213332", "issn": "0021-9738", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090709-100213332", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Ministerio de Ciencia e Innovaci\u00f3n (MCINN)", "grant_number": "BFU2005-05772" }, { "agency": "Ministerio de Ciencia e Innovaci\u00f3n (MCINN)", "grant_number": "BFU2008-01042" }, { "agency": "Ministerio de Ciencia e Innovaci\u00f3n (MCINN)", "grant_number": "NAN2004-09230-C04-04" }, { "agency": "Ministerio de Ciencia e Innovaci\u00f3n (MCINN)", "grant_number": "CONSOLIDER-INGENIO 2010 CSD2007-00017" }, { "agency": "Ministerio de Ciencia e Innovaci\u00f3n (MCINN)", "grant_number": "CSD2007-00023" }, { "agency": "Generalitat Valenciana", "grant_number": "Prometeo/2008/049" }, { "agency": "NIH", "grant_number": "R01 DE017911" }, { "agency": "NIH", "grant_number": "P50HG004071" }, { "agency": "I3P Programme (European Social Fund/MEC)" }, { "agency": "Gordon and Betty Moore Foundation" } ] }, "doi": "10.1172/JCI38019", "pmcid": "PMC2689100", "primary_object": { "basename": "Acloque2009p4429Journal_of_Clinical_Investigation.pdf", "url": "https://authors.library.caltech.edu/records/xdfpd-hbc04/files/Acloque2009p4429Journal_of_Clinical_Investigation.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "Acloque, Herv\u00e9; Adams, Meghan S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zg1gw-vc964", "eprint_id": 14606, "eprint_status": "archive", "datestamp": "2023-08-20 01:49:07", "lastmod": "2023-10-18 18:08:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Adams-M-S", "name": { "family": "Adams", "given": "Meghan Sara" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Review: the role of neural crest cells in the endocrine system", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2009 Humana Press Inc. \n\nPublished online: 18 April 2009.", "abstract": "The neural crest is a pluripotent population of cells that arises at the junction of the neural tube and the dorsal ectoderm. These highly migratory cells form diverse derivatives including neurons and glia of the sensory, sympathetic, and enteric nervous systems, melanocytes, and the bones, cartilage, and connective tissues of the face. The neural crest has long been associated with the endocrine system, although not always correctly. According to current understanding, neural crest cells give rise to the chromaffin cells of the adrenal medulla, chief cells of the extra-adrenal paraganglia, and thyroid C cells. The endocrine tumors that correspond to these cell types are pheochromocytomas, extra-adrenal paragangliomas, and medullary thyroid carcinomas. Although controversies concerning embryological origin appear to have mostly been resolved, questions persist concerning the pathobiology of each tumor type and its basis in neural crest embryology. Here we present a brief history of the work on neural crest development, both in general and in application to the endocrine system. In particular, we present findings related to the plasticity and pluripotency of neural crest cells as well as a discussion of several different neural crest tumors in the endocrine system.", "date": "2009-06", "date_type": "published", "publication": "Endocrine Pathology", "volume": "20", "number": "2", "publisher": "Humana Press", "pagerange": "92-100", "id_number": "CaltechAUTHORS:20090717-102637892", "issn": "1559-0097", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090717-102637892", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1007/s12022-009-9070-6", "resource_type": "article", "pub_year": "2009", "author_list": "Adams, Meghan Sara and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/kp8z3-5vn69", "eprint_id": 14436, "eprint_status": "archive", "datestamp": "2023-08-21 21:25:41", "lastmod": "2023-10-18 17:59:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Birth of ophthalmic trigeminal neurons initiates early in the placodal ectoderm", "ispublished": "pub", "full_text_status": "public", "keywords": "trigeminal placode; BrdU; thymidine; birthdate; neuron", "note": "\u00a9 2009 John Wiley & Sons, Inc. \n\nReceived 17 October 2008; Revised 27 November 2008; Accepted 15\nJanuary 2009. Published Online: 5 March 2009. \n\nGrant sponsor: National Institutes of Health; Grant number: R01DE16459. \n\nWe thank Samuel Ki, Virginia Satterfield, and Dr. Vivian Lee for technical assistance.\n\nPublished - McCabe2009p74710.1002cne.22004supp4.tiff
Accepted Version - nihms113945.pdf
Supplemental Material - McCabe2009p74710.1002cne.22004supp1.tiff
Supplemental Material - McCabe2009p74710.1002cne.22004supp2.tiff
Supplemental Material - McCabe2009p74710.1002cne.22004supp3.tiff
Supplemental Material - McCabe2009p74710.1002cne.22004supp5.tiff
", "abstract": "The largest of the cranial ganglia, the trigeminal ganglion, relays cutaneous sensations of the head to the central nervous system. Its sensory neurons have a dual origin from both ectodermal placodes and neural crest. Here, we show that the birth of neurons derived from the chick ophthalmic trigeminal placode begins prior to their ingression (HH11), as early as HH8, and considerably earlier than previously suspected (HH16). Furthermore, cells exiting the cell cycle shortly thereafter express the ophthalmic trigeminal placode marker Pax3 (HH9). At HH11, these postmitotic Pax3+ placode cells begin to express the pan-neuronal marker neurofilament while still in the ectoderm. Analysis of the ectodermal origin and distribution of these early postmitotic neurons reveals that the ophthalmic placode extends further rostrally than anticipated, contributing to neurons that reside in and make a significant contribution to the ophthalmic trigeminal nerve. These data redefine the timing and extent of neuron formation from the ophthalmic trigeminal placode.", "date": "2009-05-10", "date_type": "published", "publication": "Journal of Comparative Neurology", "volume": "514", "number": "2", "publisher": "Wiley", "pagerange": "161-173", "id_number": "CaltechAUTHORS:20090624-090403491", "issn": "0021-9967", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090624-090403491", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE1 6459" } ] }, "doi": "10.1002/cne.22004", "pmcid": "PMC2717094", "primary_object": { "basename": "McCabe2009p74710.1002cne.22004supp4.tiff", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/McCabe2009p74710.1002cne.22004supp4.tiff" }, "related_objects": [ { "basename": "McCabe2009p74710.1002cne.22004supp5.tiff", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/McCabe2009p74710.1002cne.22004supp5.tiff" }, { "basename": "nihms113945.pdf", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/nihms113945.pdf" }, { "basename": "McCabe2009p74710.1002cne.22004supp1.tiff", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/McCabe2009p74710.1002cne.22004supp1.tiff" }, { "basename": "McCabe2009p74710.1002cne.22004supp2.tiff", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/McCabe2009p74710.1002cne.22004supp2.tiff" }, { "basename": "McCabe2009p74710.1002cne.22004supp3.tiff", "url": "https://authors.library.caltech.edu/records/kp8z3-5vn69/files/McCabe2009p74710.1002cne.22004supp3.tiff" } ], "resource_type": "article", "pub_year": "2009", "author_list": "McCabe, Kathryn L.; Sechrist, John W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sa4gd-53z53", "eprint_id": 40915, "eprint_status": "archive", "datestamp": "2023-08-21 21:25:03", "lastmod": "2023-10-24 23:17:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Chao-Jennifer-R", "name": { "family": "Chao", "given": "J. R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" }, { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "P. Y." } } ] }, "title": "Stem-Cell Properties of Human Corneal Keratocytes", "ispublished": "pub", "full_text_status": "public", "keywords": "cornea: stroma and keratocytes; development; cornea: basic science", "note": "\u00a9 2009 Association for Research in Vision and Ophthalmology. \n\nSupport: Knights Templar Eye Foundation (JRC), Fight for Sight (JRC), NIH grants (MBF), NIH K99/R00 grant EY018050 (PYL).\n\nPublished - Chao_2009p2049.pdf
", "abstract": "Purpose: To determine the stem cell properties of human corneal stromal keratocytes when\nchallenged in the chick embryonic environment.\nMethods: Stromal keratocytes isolated from human corneas were injected along cranial neural\ncrest migratory pathways and in the periocular mesenchyme in chick embryos. Localization\nMigration of the injected cells stromal keratocytes was determined at various stages of\ndevelopment by immunohistochemistry using human cell-specific markers. Differentiation of the\nhuman keratocytes into other neural crest-derived tissues was determined by\nimmunohistochemistry with tissue cell-specific markers.\nResults: Human keratocytes injected along cranial neural crest pathways proliferated and migrated\nventrally adjacent to host neural crest cells. They contributed to numerous neural crest-derived\ntissues including cranial blood vessels, ocular tissues, and cardiac cushion tissue mesenchyme.\nKeratocytes injected into the periocular mesenchyme region contributed to the corneal stroma and\nendothelial layers.\nConclusions: Adult human corneal stromal keratocytes exhibit stem cell characteristics. They can\nbe induced to form cranial neural crest derivatives, including other anterior ocular structures, when\ngrafted into an embryonic environment.", "date": "2009-05-04", "date_type": "published", "publication": "Investigative Ophthalmology and Visual Science", "volume": "50", "publisher": "Association for Research in Vision and Ophthalmology", "pagerange": "2049", "id_number": "CaltechAUTHORS:20130826-081021118", "issn": "1552-5783", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130826-081021118", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Knights Templar Eye Foundation" }, { "agency": "Fight for Sight" }, { "agency": "NIH", "grant_number": "K99/R00 EY018050" } ] }, "primary_object": { "basename": "Chao_2009p2049.pdf", "url": "https://authors.library.caltech.edu/records/sa4gd-53z53/files/Chao_2009p2049.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "Chao, J. R.; Bronner-Fraser, M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jrzdg-4b435", "eprint_id": 15750, "eprint_status": "archive", "datestamp": "2023-08-21 21:09:20", "lastmod": "2023-10-19 14:49:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya V." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Gene regulatory networks that control the specification of neural-crest cells in the lamprey", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Gene regulatory network; Neural crest; Lamprey", "note": "\u00a9 2008 Elsevier B.V. \n\nReceived 14 January 2008. Received in revised form 4 March 2008. Accepted 18 March 2008. Available online 30 March 2008.", "abstract": "The lamprey is the only basal vertebrate in which large-scale gene perturbation analyses are feasible at present. Studies on this unique animal model promise to contribute both to the understanding of the basic neural-crest gene regulatory network architecture, and evolution of the neural crest. In this review, we summarize the currently known regulatory relationships underlying formation of the vertebrate neural crest, and discuss new ways of addressing the many remaining questions using lamprey as an experimental model.", "date": "2009-04", "date_type": "published", "publication": "Biochimica et Biophysica Acta - Gene Regulatory Mechanisms", "volume": "1789", "number": "4", "publisher": "Elsevier", "pagerange": "274-278", "id_number": "CaltechAUTHORS:20090911-075415531", "issn": "1874-9399", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090911-075415531", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.bbagrm.2008.03.006", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya V. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0d5p4-wxx81", "eprint_id": 13965, "eprint_status": "archive", "datestamp": "2023-08-21 20:52:30", "lastmod": "2023-10-18 16:00:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Khudyakov-J", "name": { "family": "Khudyakov", "given": "Jane" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Comprehensive spatiotemporal analysis of early chick neural crest network genes", "ispublished": "pub", "full_text_status": "public", "keywords": "chick neural crest; neural plate border; gene regulatory network; NC-GRN", "note": "\u00a9 2009 Wiley-Liss, Inc. \n\nAccepted 24 December 2008. \n\nWe thank Tatjana Sauka-Spengler, Sujata Bhattacharyya, Meyer Barembaum, and Jack Sechrist for their technical advice and discussion. M.B.-F. was funded by the NIH.\n\nAccepted Version - nihms90805.pdf
", "abstract": "Specification of neural crest progenitors begins during gastrulation at the neural plate border, long before migration or differentiation. Neural crest cell fate is acquired by progressive activation of discrete groups of transcription factors that appear to be highly conserved in vertebrates; however, comprehensive analysis of their expression has been lacking in chick, an important model system for neural crest development. To address this, we analyzed expression of 10 transcription factors that are known specifiers of neural plate border and neural crest fate and compared them across developmental stages from gastrulation to neural crest migration. Surprisingly, we find that most neural crest specifiers are expressed during gastrulation in chick, concomitant with and in similar domains as neural plate border specifiers. This finding suggests that interactions between these molecules may occur much earlier than previously thought, an important consideration for interpretation of functional studies.", "date": "2009-03", "date_type": "published", "publication": "Developmental Dynamics", "volume": "238", "number": "3", "publisher": "Wiley-Liss, Inc.", "pagerange": "716-723", "id_number": "CaltechAUTHORS:20090414-091753664", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090414-091753664", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1002/dvdy.21881", "pmcid": "PMC2650819", "primary_object": { "basename": "nihms90805.pdf", "url": "https://authors.library.caltech.edu/records/0d5p4-wxx81/files/nihms90805.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "Khudyakov, Jane and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6xkjy-myd48", "eprint_id": 16213, "eprint_status": "archive", "datestamp": "2023-08-20 00:48:34", "lastmod": "2023-10-19 22:02:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Gene regulatory network underlying neural crest formation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Blackwell Publishing Ltd.\n\nPublished - BronnerFraser2009p342.pdf
", "abstract": "[no abstract]", "date": "2009-02", "date_type": "published", "publication": "Neurogastroenterology and Motility", "volume": "21", "number": "2", "publisher": "Blackwell", "pagerange": "i", "id_number": "CaltechAUTHORS:20091008-081738584", "issn": "1350-1925", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20091008-081738584", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "BronnerFraser2009p342.pdf", "url": "https://authors.library.caltech.edu/records/6xkjy-myd48/files/BronnerFraser2009p342.pdf" }, "resource_type": "article", "pub_year": "2009", "author_list": "Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9em09-8c659", "eprint_id": 63358, "eprint_status": "archive", "datestamp": "2023-08-20 00:37:15", "lastmod": "2023-10-25 23:45:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "The Sea Lamprey Petromyzon marinus: A Model for Evolutionary and Developmental Biology", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Sea lampreys (Petromyzon marinus) are cyclostomes, the most basal extant group of vertebrates, and are thought to have existed largely unchanged for more than 500 million years. They are aquatic, eel-shaped animals that spend a major part of their life as filter-feeding larvae called ammocoetes, inhabiting many freshwater bodies in the northern hemisphere. After metamorphosis, sea lampreys migrate to the ocean (or to the Great Lakes), where they feed on the blood and bodily fluids of salmonid fish and ultimately return to freshwater streams and rivers to spawn and die. The unique evolutionary position of lampreys and the relative ease of obtaining mature adults and embryos make this animal an ideal model for investigations into early vertebrate evolution. Studies of features shared between lampreys and jawed vertebrates, but distinct from those in nonvertebrate chordates, have provided information on the origin and evolution of hallmark vertebrate characteristics such as the neural crest, ectodermal placodes, and jaw. In addition, studies of features that are unique to lampreys (e.g., the variable lymphocyte receptor-mediated immune system) provide insights into mechanisms of parallel evolution (e.g., the adaptive immune system). With the establishment of techniques for the extended maintenance and spawning of lampreys in the laboratory, the sequencing of the lamprey genome, and the adaptation and optimization of many established molecular biology and histochemistry techniques for use in this species, P. marinus is poised to become an evolutionary developmental model of choice.", "date": "2009-01", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "number": "1", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.emo113", "id_number": "CaltechAUTHORS:20160104-183306659", "issn": "1559-6095", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183306659", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.emo113", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3427s-f0f17", "eprint_id": 63359, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:16", "lastmod": "2023-10-25 23:45:26", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "Culturing Lamprey Embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. This protocol describes how to produce lamprey embryos by collecting sperm and eggs from mature lampreys, performing fertilization, and culturing the embryos through to the desired developmental stage.", "date": "2009", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "number": "1", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.prot5122", "id_number": "CaltechAUTHORS:20160104-183306970", "issn": "1940-3402", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183306970", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.prot5122", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fyrpj-k8q54", "eprint_id": 63361, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:23", "lastmod": "2023-10-25 23:45:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "DiI Cell Labeling in Lamprey Embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. Lamprey embryos are particularly amenable to injection techniques. Like zebrafish and Xenopus embryos, they have double chorions and are resistant to surface-tension-induced rupture when removed from liquid. They can therefore be injected in a dry dish; this eliminates the need to support the embryo while performing injections and makes the procedure very rapid. Also, a single ovulating female can contain up to 100,000 eggs, so the number of injectable embryos per fertilization is not a limiting factor. This protocol describes how to label lamprey embryo cells by microinjecting the fluorescent dye DiI (1,1\u2032-dioctadecyl-3,3,3\u2032,3\u2032-tetramethylindocarbocyanine perchlorate) to study cell fate during development.", "date": "2009", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "number": "1", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.prot5124", "id_number": "CaltechAUTHORS:20160104-183307486", "issn": "1559-6095", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183307486", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.prot5124", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/677j2-sv256", "eprint_id": 15624, "eprint_status": "archive", "datestamp": "2023-08-22 13:57:43", "lastmod": "2024-01-12 23:37:41", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Gene Regulatory Networks in Neural Crest Development and Evolution", "ispublished": "unpub", "full_text_status": "restricted", "keywords": "neural crest; evolution and development", "note": "\u00a9 2009 Elsevier Inc. \n\nAvailable online 8 April 2009.", "abstract": "The neural crest is a multipotent migratory embryonic cell population that is present in all vertebrates, but missing from basal chordates. In this chapter, we discuss recent work in amphioxus, ascidians, lamprey, and gnathostomes that reflects the current state of knowledge of the evolutionary origin of this fascinating cell population. We summarize recent evidence for the ongoing diversification of the neural crest in several vertebrate species, with particular reference to studies in nontraditional vertebrate model organisms.", "date": "2009", "date_type": "published", "publisher": "Elsevier", "place_of_pub": "Amsterdam", "pagerange": "1-14", "id_number": "CaltechAUTHORS:20090904-133612122", "isbn": "978-0-12-374455-5", "book_title": "Evolution and Development", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090904-133612122", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0070-2153(09)01001-1", "resource_type": "book_section", "pub_year": "2009", "author_list": "Nikitina, Natalya; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/en45e-fhd17", "eprint_id": 63363, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:29", "lastmod": "2023-10-25 23:45:36", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "Immunostaining of Whole-Mount and Sectioned Lamprey Embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. This protocol describes how to immunostain whole-mount or sectioned lamprey embryos using an antibody raised against the protein of interest and detected with a horseradish peroxidase (HRP)-conjugated secondary antibody.", "date": "2009", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "number": "1", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.prot5126", "id_number": "CaltechAUTHORS:20160104-183308021", "issn": "1940-3402", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183308021", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.prot5126", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2k2yn-e2x28", "eprint_id": 63360, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:19", "lastmod": "2023-10-25 23:45:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "Microinjection of RNA and Morpholino Oligos into Lamprey Embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. Lamprey embryos are particularly amenable to injection techniques. Like zebrafish and Xenopus embryos, they have double chorions and are resistant to surface-tension-induced rupture when removed from liquid. They can therefore be injected in a dry dish; this eliminates the need to support the embryo while performing injections and makes the procedure very rapid. Also, a single ovulating female can contain up to 100,000 eggs, so the number of injectable embryos per fertilization is not a limiting factor. Finally, the second division lasts for several hours, providing a very large injection window. This protocol describes how to microinject RNA and morpholinos into lamprey embryos for genetic modification studies.", "date": "2009", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "number": "1", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.prot5123", "id_number": "CaltechAUTHORS:20160104-183307215", "issn": "1940-3402", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183307215", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.prot5123", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nzd6x-8y126", "eprint_id": 63451, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:33", "lastmod": "2023-10-25 23:49:53", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "The Sea Lamprey Petromyzon marinus: A Model for Evolutionary and Developmental Biology", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Sea lampreys (Petromyzon marinus) are cyclostomes, the most basal extant group of vertebrates, and are thought to have existed largely unchanged for more than 500 million years. They are aquatic, eel-shaped animals that spend a major part of their life as filter-feeding larvae called ammocoetes, inhabiting many freshwater bodies in the northern hemisphere. After metamorphosis, sea lampreys migrate to the ocean (or to the Great Lakes), where they feed on the blood and bodily fluids of salmonid fish and ultimately return to freshwater streams and rivers to spawn and die. The unique evolutionary position of lampreys and the relative ease of obtaining mature adults and embryos make this animal an ideal model for investigations into early vertebrate evolution. Studies of features shared between lampreys and jawed vertebrates, but distinct from those in nonvertebrate chordates, have provided information on the origin and evolution of hallmark vertebrate characteristics such as the neural crest, ectodermal placodes, and jaw. In addition, studies of features that are unique to lampreys (e.g., the variable lymphocyte receptor-mediated immune system) provide insights into mechanisms of parallel evolution (e.g., the adaptive immune system). With the establishment of techniques for the extended maintenance and spawning of lampreys in the laboratory, the sequencing of the lamprey genome, and the adaptation and optimization of many established molecular biology and histochemistry techniques for use in this species, P. marinus is poised to become an evolutionary developmental model of choice.", "date": "2009", "date_type": "published", "publisher": "Cold Spring Harbor Laboratory Press", "place_of_pub": "Cold Spring Harbor, NY", "pagerange": "405-429", "id_number": "CaltechAUTHORS:20160107-120548257", "isbn": "0879698268", "book_title": "Emerging model organisms : a laboratory manual", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160107-120548257", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "book_section", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ae0vn-x8141", "eprint_id": 63362, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:26", "lastmod": "2023-10-25 23:45:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" } ] }, "title": "Whole-Mount In Situ Hybridization on Lamprey Embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2009 Cold Spring Harbor Laboratory Press.", "abstract": "Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. This protocol describes an optimized procedure for RNA in situ hybridization in lamprey embryos.", "date": "2009", "date_type": "published", "publication": "Cold Spring Harbor Protocols", "volume": "2009", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "Art. No. pdb.prot5125", "id_number": "CaltechAUTHORS:20160104-183307776", "issn": "1940-3402", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160104-183307776", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1101/pdb.prot5125", "resource_type": "article", "pub_year": "2009", "author_list": "Nikitina, Natalya; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2qjwx-7ps04", "eprint_id": 13016, "eprint_status": "archive", "datestamp": "2023-08-22 13:55:23", "lastmod": "2023-10-17 21:42:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nikitina-N", "name": { "family": "Nikitina", "given": "Natalya" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dissecting early regulatory relationships in the lamprey neural crest gene network", "ispublished": "pub", "full_text_status": "public", "keywords": "neural plate border; transcription factor; agnathan", "note": "\u00a9 2008 by The National Academy of Sciences of the USA. \n\nEdited by Michael S. Levine, University of California, Berkeley, CA, and approved October 21, 2008 (received for review June 20, 2008). This article is a PNAS Direct Submission. Published online before print December 22, 2008, doi: 10.1073/pnas.0806009105 \n\nThis work was supported by the National Institutes of Health Grant DE017911 (to M.B.F.). \n\nThis paper results from the Arthur M. Sackler Colloquium of the National Academy of Sciences, \"Gene Networks in Animal Development and Evolution,\" held February 15\u201316, 2008, at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering in Irvine, CA. The complete program and audio files of most presentations are available on the NAS web site at http://www.nasonline.org/SACKLER_Gene_Networks. \n\nAuthor contributions: N.N., T.S.-S., and M.B.-F. designed research; N.N. and T.S.-S. performed research; T.S.-S. contributed new reagents/analytic tools; N.N. and T.S.-S. analyzed data; and N.N., T.S.-S., and M.B.-F. wrote the paper. \n\nThe authors declare no conflict of interest. \n\nThis article contains supporting information online at www.pnas.org/cgi/content/full/0806009105/DCSupplemental.\n\nPublished - NIKpnas08.pdf
Supplemental Material - NIKpnas08supp.pdf
", "abstract": "The neural crest, a multipotent embryonic cell type, originates at the border between neural and nonneural ectoderm. After neural tube closure, these cells undergo an epithelial\u2013mesenchymal transition, migrate to precise, often distant locations, and differentiate into diverse derivatives. Analyses of expression and function of signaling and transcription factors in higher vertebrates has led to the proposal that a neural crest gene regulatory network (NC-GRN) orchestrates neural crest formation. Here, we interrogate the NC-GRN in the lamprey, taking advantage of its slow development and basal phylogenetic position to resolve early inductive events, 1 regulatory step at the time. To establish regulatory relationships at the neural plate border, we assess relative expression of 6 neural crest network genes and effects of individually perturbing each on the remaining 5. The results refine an upstream portion of the NC-GRN and reveal unexpected order and linkages therein; e.g., lamprey AP-2 appears to function early as a neural plate border rather than a neural crest specifier and in a pathway linked to MsxA but independent of ZicA. These findings provide an ancestral framework for performing comparative tests in higher vertebrates in which network linkages may be more difficult to resolve because of their rapid development.", "date": "2008-12-23", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "105", "number": "51", "publisher": "National Academy of Sciences", "pagerange": "20083-20088", "id_number": "CaltechAUTHORS:NIKpnas08", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:NIKpnas08", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1073/pnas.0806009105", "pmcid": "PMC2629288", "primary_object": { "basename": "NIKpnas08supp.pdf", "url": "https://authors.library.caltech.edu/records/2qjwx-7ps04/files/NIKpnas08supp.pdf" }, "related_objects": [ { "basename": "NIKpnas08.pdf", "url": "https://authors.library.caltech.edu/records/2qjwx-7ps04/files/NIKpnas08.pdf" } ], "resource_type": "article", "pub_year": "2008", "author_list": "Nikitina, Natalya; Sauka-Spengler, Tatjana; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/s6exn-gmg54", "eprint_id": 12509, "eprint_status": "archive", "datestamp": "2023-08-22 13:52:15", "lastmod": "2023-10-17 17:01:41", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Competence, specification and commitment to an olfactory placode fate", "ispublished": "pub", "full_text_status": "public", "keywords": "Olfactory placode, Nasal placode, Chick, Competence, Specification, Commitment, Induction, Ectoderm", "note": "\u00a9 The Company of Biologists Ltd 2008. \n\nAccepted 17 October 2008. First published online November 21, 2008. \n\nWe thank Drs Andy Groves and Michael Kessel for cDNA clones, Dr Jhumku Kohtz for the pan-Dlx antibody, Dr James Millam for the chick GnRH-I antibody, Dr Andy Kowalczyk for use of his microscope, and Matt Jones, David Arce and Andrea Manzo for technical assistance. We are grateful to Dr Tatjana Sauka-Spengler for critical reading of the manuscript and to Dr Andrea Streit for many helpful discussions. This work was supported by DE16459 to M.B.-F. and partly by a HHMI Predoctoral Fellowship to S.B.\n\nPublished - BHAdev08.pdf
", "abstract": "The nasal placode shares a common origin with other sensory placodes within a pre-placodal domain at the cranial neural plate border. However, little is known about early events in nasal placode development as it segregates from prospective lens, neural tube and epidermis. Here, Dlx3, Dlx5, Pax6 and the pan-neuronal marker Hu serve as molecular labels to follow the maturation of olfactory precursors over time. When competence to form olfactory placode was tested by grafting ectoderm from different axial levels to the anterior neural fold, we found that competence is initially broad for head, but not trunk, ectoderm and declines rapidly with time. Isolated olfactory precursors are specified by HH10, concomitant with their complete segregation from other placodal, epidermal and neural progenitors. Heterotopic transplantation of olfactory progenitors reveals they are capable of autonomous differentiation only 12 hours later, shortly before overt placode invagination at HH14. Taken together, these results show that olfactory placode development is a step-wise process whereby signals from adjacent tissues specify competent ectoderm at or before HH10, followed by gradual commitment just prior to morphological differentiation.", "date": "2008-12-15", "date_type": "published", "publication": "Development", "volume": "135", "number": "24", "publisher": "Company of Biologists", "pagerange": "4165-4177", "id_number": "CaltechAUTHORS:BHAdev08", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BHAdev08", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE16459" }, { "agency": "Howard Hughes Medical Institute (HHMI)" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1242/10.1242/dev.026633", "primary_object": { "basename": "BHAdev08.pdf", "url": "https://authors.library.caltech.edu/records/s6exn-gmg54/files/BHAdev08.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "Bhattacharyya, Sujata and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/aygwt-sjp79", "eprint_id": 12868, "eprint_status": "archive", "datestamp": "2023-08-22 13:28:29", "lastmod": "2023-10-17 21:09:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolution of the neural crest viewed from a gene regulatory perspective", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest; gene regulation; network; evolution; development", "note": "\u00a9 2008 Wiley-Liss, Inc. \n\nReceived: 14 May 2008; Revised: 18 July 2008; Accepted: 12 August 2008. Published online 10 November 2008.", "abstract": "Neural crest cells are a vertebrate innovation and form a wide variety of embryonic cell types as diverse as peripheral neurons and facial skeleton. They undergo complex migration and differentiation processes from their site of origin in the developing central nervous system to their final destinations in the periphery. In this review, we summarize recent data on the current formulation of a gene regulatory network underlying neural crest formation and its roots at the base of the vertebrate lineage. Analyzing neural crest formation from a gene regulatory viewpoint provides insights into both the developmental mechanisms and evolutionary origins of this vertebrate-specific cell type.", "date": "2008-11", "date_type": "published", "publication": "Genesis", "volume": "46", "number": "11", "publisher": "Wiley", "pagerange": "673-682", "id_number": "CaltechAUTHORS:SAUgen08", "issn": "1526-954X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:SAUgen08", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/dvg.20436", "resource_type": "article", "pub_year": "2008", "author_list": "Sauka-Spengler, Tatjana and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ss2f4-4rq41", "eprint_id": 14124, "eprint_status": "archive", "datestamp": "2023-08-19 23:38:53", "lastmod": "2023-10-18 16:08:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "On the trail of the 'new head' in Les Treilles", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2008 The Company of Biologists. \n\nFirst published online August 25, 2008.\n\nPublished - BROdev08.pdf
", "abstract": "The vertebrate brain develops in association with neighboring tissues: neural crest, placodes, mesoderm and endoderm. The molecular and evolutionary relationships between the forming nervous system and the other craniofacial structures were at the focus of a recent meeting at the Fondation des Treilles in France. Entitled 'Relationships between Craniofacial and Neural Development', the meeting brought together researchers working on diverse species, the findings of whom provide clues as to the origin and diversity of the brain and facial regions that are involved in forming the 'new head' of vertebrates.", "date": "2008-09-15", "date_type": "published", "publication": "Development", "volume": "135", "number": "18", "publisher": "Company of Biologists", "pagerange": "2995-2999", "id_number": "CaltechAUTHORS:20090430-130815333", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090430-130815333", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1242/10.1242/dev.019901", "primary_object": { "basename": "BROdev08.pdf", "url": "https://authors.library.caltech.edu/records/ss2f4-4rq41/files/BROdev08.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8ptat-n5e05", "eprint_id": 64509, "eprint_status": "archive", "datestamp": "2023-08-22 12:56:40", "lastmod": "2023-10-17 21:27:32", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coles-E-G", "name": { "family": "Coles", "given": "Edward G." } }, { "id": "Lawlor-E-R", "name": { "family": "Lawlor", "given": "Elizabeth R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "EWS-FLI1 Causes Neuroepithelial Defects and Abrogates Emigration of Neural Crest Stem Cells", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest stem cell; Ewing's sarcoma; EWS-FLI1; Chick embryo", "note": "\u00a9 2008 AlphaMed Press. \n\nArticle first published online: 12 Jun 2008. \n\nWe thank Lisa Taneyhill for providing valuable resources and facilitating the completion of this paper, and David Arce for technical support. This work was supported by the American Heart Association (0525037Y to E.G.C.); a V Foundation Scholar Award and American Cancer Society Institutional research grant (IRG-58\u2013007-IRG) and California Institute for Regenerative Medicine SEED grant (RS 00249), both to E.R.L.; and the NIH (NS36585 to M.B.-F.).", "abstract": "The most frequently occurring chromosomal translocation that gives rise to the Ewing's sarcoma family of tumors (ESFT) is the chimeric fusion gene EWS-FLI1 that encodes an oncogenic protein composed of the N terminus of EWS and the C terminus of FLI1. Although the genetic basis of ESFT is fairly well understood, its putative cellular origin remains to be determined. Previous work has proposed that neural crest progenitor cells may be the causative cell type responsible for ESFT. However, surprisingly little is known about the expression pattern or role of either wild-type EWS or wild-type FLI1 in this cell population during early embryonic development. Using the developing chick embryo as a model system, we identified EWS expression in emigrating and migratory neural crest stem cells, whereas FLI1 transcripts were found to be absent in these populations and were restricted to developing endothelial cells. By ectopically expressing EWS-FLI1 or wild-type FLI1 in the developing embryo, we have been able to study the cellular transformations that ensue in the context of an in vivo model system. Our results reveal that misexpression of the chimeric EWS-FLI1 fusion gene, or wild-type FLI1, in the developing neural crest stem cell population leads to significant aberrations in neural crest development. An intriguing possibility is that misexpression of the EWS-FLI1 oncogene in neural crest-derived stem cells may be an initiating event in ESFT genesis.", "date": "2008-09", "date_type": "published", "publication": "Stem Cells", "volume": "26", "number": "9", "publisher": "Wiley", "pagerange": "2237-2244", "id_number": "CaltechAUTHORS:20160216-122510801", "issn": "1066-5099", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160216-122510801", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "0525037Y" }, { "agency": "V Foundation for Cancer Research" }, { "agency": "American Cancer Society", "grant_number": "IRG-58\u2013007-IRG" }, { "agency": "California Institute for Regenerative Medicine (CIRM)", "grant_number": "RS-00249" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1634/stemcells.2008-0133", "resource_type": "article", "pub_year": "2008", "author_list": "Coles, Edward G.; Lawlor, Elizabeth R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dda8r-q1898", "eprint_id": 64830, "eprint_status": "archive", "datestamp": "2023-08-19 23:03:19", "lastmod": "2023-10-17 21:54:17", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A gene regulatory network orchestrates neural crest formation", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2008 Macmillan Publishers Limited. \n\nPublished online 4 June 2008. \n\nWe thank S. Bhattacharyya and N. Nikitina for their suggestions and critical reading of the manuscript. This work was supported by California Institute for Regenerative Medicine (CIRM) fellowship to T.S.S. and National Institute of Health (NIH) grants, DE017919 and NS36585, to M.B.F.", "abstract": "The neural crest is a multipotent, migratory cell population that is unique to vertebrate embryos and gives rise to many derivatives, ranging from the peripheral nervous system to the craniofacial skeleton and pigment cells. A multimodule gene regulatory network mediates the complex process of neural crest formation, which involves the early induction and maintenance of the precursor pool, emigration of the neural crest progenitors from the neural tube via an epithelial to mesenchymal transition, migration of progenitor cells along distinct pathways and overt differentiation into diverse cell types. Here, we review our current understanding of these processes and discuss the molecular players that are involved in the neural crest gene regulatory network.", "date": "2008-07", "date_type": "published", "publication": "Nature Reviews. Molecular Cell Biology", "volume": "9", "number": "7", "publisher": "Nature Publishing Group", "pagerange": "557-568", "id_number": "CaltechAUTHORS:20160229-073228033", "issn": "1471-0072", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160229-073228033", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "California Institute for Regenerative Medicine (CIRM)" }, { "agency": "NIH", "grant_number": "DE017919" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1038/nrm2428", "resource_type": "article", "pub_year": "2008", "author_list": "Sauka-Spengler, Tatjana and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nt4va-0gq85", "eprint_id": 64184, "eprint_status": "archive", "datestamp": "2023-08-19 23:03:12", "lastmod": "2023-10-17 19:34:58", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Yu-Jr-Kai", "name": { "family": "Yu", "given": "Jr-Kai" } }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Insights from the amphioxus genome on the origin of vertebrate neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2008, Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). \n\nReceived January 23, 2008; accepted in revised form March 13, 2008. \n\nWe thank Linda Holland, Noriyuki Satoh, Yutaka Satou, and Yuji Kohara for the amphioxus EST resources, and the Joint Genome Institute for the amphioxus genome sequence resources. We also thank John Lawrence, Susan Bell, Ray Martinez Jr., and James Swigart at the University of South Florida for providing laboratory facilities during the summer breeding season of amphioxus. We thank Hisato Kondoh for donating the ptkEGFP vector and Tatjana Sauka-Spengler for providing the pRFP-H2B plasmid. This work was funded by a grant from National Institutes of Health DE017911 (M.B.-F). J.-K.Y was supported by the Della Martin prize postdoctoral fellowship from the Division of Biology, California Institute of Technology. S.J.M. is supported by an Australian Government NH&MRC CJ Martin fellowship.\n\nPublished - 1127.full.pdf
", "abstract": "The emergence of the neural crest has been proposed to play a key role in early vertebrate evolution by remodeling the chordate head into a \"new head\" that enabled early vertebrates to shift from filter feeding to active predation. Here we show that the genome of the basal chordate, amphioxus, contains homologs of most vertebrate genes implicated in a putative neural crest gene regulatory network (NC-GRN) for neural crest development. Our survey of gene expression shows that early inducing signals, neural plate border patterning genes, and melanocyte differentiation genes appear conserved. Furthermore, exogenous BMP affects expression of amphioxus neural plate border genes as in vertebrates, suggesting that conserved signals specify the neural plate border throughout chordates. In contrast to this core conservation, many neural crest specifier genes are not expressed at the amphioxus neural plate/tube border, raising the intriguing possibility that this level of the network was co-opted during vertebrate evolution. Consistent with this, the regulatory region of AmphiFoxD, homologous to the vertebrate neural crest specifier FoxD3, drives tissue-specific reporter expression in chick mesoderm, but not neural crest. Thus, evolution of a new regulatory element may have allowed co-option of this gene to the NC-GRN.", "date": "2008-07", "date_type": "published", "publication": "Genome Research", "volume": "18", "number": "7", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "1127-1132", "id_number": "CaltechAUTHORS:20160203-090815347", "issn": "1088-9051", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160203-090815347", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" }, { "agency": "Caltech Division of Biology" }, { "agency": "National Health and Medical Research Council (NHMRC)" } ] }, "doi": "10.1101/gr.076208.108", "pmcid": "PMC2493401", "primary_object": { "basename": "1127.full.pdf", "url": "https://authors.library.caltech.edu/records/nt4va-0gq85/files/1127.full.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "Yu, Jr-Kai; Meulemans, Daniel; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qea1q-7ay26", "eprint_id": 11220, "eprint_status": "archive", "datestamp": "2023-09-14 16:41:55", "lastmod": "2023-10-23 20:41:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Putnam-N-H", "name": { "family": "Putnam", "given": "Nicholas H." } }, { "id": "Butts-T", "name": { "family": "Butts", "given": "Thomas" } }, { "id": "Ferrier-D-E-K", "name": { "family": "Ferrier", "given": "David E. K." } }, { "id": "Furlong-R-F", "name": { "family": "Furlong", "given": "Rebecca F." } }, { "id": "Hellsten-U", "name": { "family": "Hellsten", "given": "Uffe" } }, { "id": "Kawashima-Takeshi", "name": { "family": "Kawashima", "given": "Takeshi" } }, { "id": "Robinson-Rechavi-M", "name": { "family": "Robinson-Rechavi", "given": "Marc" } }, { "id": "Shoguchi-Eiichi", "name": { "family": "Shoguchi", "given": "Eiichi" } }, { "id": "Terry-A", "name": { "family": "Terry", "given": "Astrid" } }, { "id": "Yu-Jr-Kai", "name": { "family": "Yu", "given": "Jr-Kai" } }, { "id": "Benito-Guti\u00e9rrez-E", "name": { "family": "Benito-Guti\u00e9rrez", "given": "\u00c8lia" } }, { "id": "Dubchak-I", "name": { "family": "Dubchak", "given": "Inna" } }, { "id": "Garcia-Fern\u00e0dez-J", "name": { "family": "Garcia-Fern\u00e0dez", "given": "Jordi" } }, { "id": "Gibson-Brown-J-J", "name": { "family": "Gibson-Brown", "given": "Jeremy J." } }, { "id": "Grigoriev-I-V", "name": { "family": "Grigoriev", "given": "Igor V." } }, { "id": "Horton-A-C", "name": { "family": "Horton", "given": "Amy C." } }, { "id": "de-Jong-P-J", "name": { "family": "de Jong", "given": "Pieter J." } }, { "id": "Jurka-J", "name": { "family": "Jurka", "given": "Jerzy" } }, { "id": "Kapitonov-V-V", "name": { "family": "Kapitonov", "given": "Vladimir V." } }, { "id": "Kohara-Yuji", "name": { "family": "Kohara", "given": "Yuji" } }, { "id": "Kuroki-Yoko", "name": { "family": "Kuroki", "given": "Yoko" } }, { "id": "Lindquist-E", "name": { "family": "Lindquist", "given": "Erika" } }, { "id": "Lucas-S", "name": { "family": "Lucas", "given": "Susan" } }, { "id": "Osoegawa-Kazutoyo", "name": { "family": "Osoegawa", "given": "Kazutoyo" } }, { "id": "Pennacchio-L-A", "name": { "family": "Pennacchio", "given": "Len A." }, "orcid": "0000-0002-8748-3732" }, { "id": "Salamov-A-A", "name": { "family": "Salamov", "given": "Asaf A." } }, { "id": "Satou-Yutaka", "name": { "family": "Satou", "given": "Yutaka" } }, { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Schmutz-J", "name": { "family": "Schumtz", "given": "Jeremy" } }, { "id": "Shin-I-Tadasu", "name": { "family": "Shin-I", "given": "Tadasu" } }, { "id": "Toyoda-Atsushi", "name": { "family": "Toyoda", "given": "Atsushi" } }, { "id": "Fujiyama-Asao", "name": { "family": "Fujiyama", "given": "Asao" } }, { "id": "Holland-L-Z", "name": { "family": "Holland", "given": "Linda Z." } }, { "id": "Holland-P-W-H", "name": { "family": "Holland", "given": "Peter W. H." } }, { "id": "Satoh-Noriyuki", "name": { "family": "Satoh", "given": "Nori" } }, { "id": "Rokhsar-D-S", "name": { "family": "Rokhsar", "given": "Daniel S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The amphioxus genome and the evolution of the chordate karyotype", "ispublished": "pub", "full_text_status": "public", "note": "This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike licence (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits distribution, and reproduction in any medium, provided the original author and source are credited. This licence does not permit commercial exploitation, and derivative works must be licensed under the same or similar licence. \n\nReceived 8 March 2008; Accepted 4 April 2008. \n\nThis work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Livermore National Laboratory under contract number W-7405-Eng-48, Lawrence Berkeley National Laboratory under contract number DE-AC02-05CH11231, and Los Alamos National Laboratory under contract number DE-AC02-06NA25396. The Center for Integrative Genomics is supported by a grant from the Gordon and Betty Moore Foundation. D.S.R. acknowledges support from R. A. Melmon. This work was funded by grants from Ministerio de Educacin y Ciencia (J.G.-F.), MEXT, Japan (N.S., A.F., and Y.K.), the 21st Century and Global COEs at Kyoto University (N.S.), grant number P41LM from the National Library of Medicine (J.J. and V.V.K.), BBSRC (T.B. and D.E.K.F.) and the Wellcome Trust (P.W.H.H.).\n\nPublished - PUTnat08.pdf
Supplemental Material - PUTnat08sup2.txt
Supplemental Material - PUTnat08sup3.zip
Supplemental Material - PUTnat08sup4.txt
Supplemental Material - PUTnat08sup5.txt
Supplemental Material - PUTnat08sup6.pdf
Supplemental Material - PUTnat08supp1.pdf
", "abstract": "Lancelets ('amphioxus') are the modern survivors of an ancient chordate lineage, with a fossil record dating back to the Cambrian period. Here we describe the structure and gene content of the highly polymorphic approx520-megabase genome of the Florida lancelet Branchiostoma floridae, and analyse it in the context of chordate evolution. Whole-genome comparisons illuminate the murky relationships among the three chordate groups (tunicates, lancelets and vertebrates), and allow not only reconstruction of the gene complement of the last common chordate ancestor but also partial reconstruction of its genomic organization, as well as a description of two genome-wide duplications and subsequent reorganizations in the vertebrate lineage. These genome-scale events shaped the vertebrate genome and provided additional genetic variation for exploitation during vertebrate evolution.", "date": "2008-06-19", "date_type": "published", "publication": "Nature", "volume": "453", "number": "7198", "publisher": "Nature Publishing Group", "pagerange": "1064-1072", "id_number": "CaltechAUTHORS:PUTnat08", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:PUTnat08", "rights": "This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike licence (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits distribution, and reproduction in any medium, provided the original author and source are credited. This licence does not permit commercial exploitation, and derivative works must be licensed under the same or similar licence.", "funders": { "items": [ { "agency": "Department of Energy (DOE)", "grant_number": "W-7405-ENG-48" }, { "agency": "Department of Energy (DOE)", "grant_number": "DE-AC02-05CH11231" }, { "agency": "Department of Energy (DOE)", "grant_number": "DE-AC02-06NA25396" }, { "agency": "Gordon and Betty Moore Foundation" }, { "agency": "R. A. Melmon" }, { "agency": "Ministerio de Educacin y Ciencia" }, { "agency": "Ministry of Education, Culture, Sports, Science and Technology (MEXT)" }, { "agency": "21st Century and Global COEs" }, { "agency": "National Library of Medicine", "grant_number": "P41LM" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)" }, { "agency": "Wellcome Trust" } ] }, "doi": "10.1038/nature06967", "primary_object": { "basename": "PUTnat08sup3.zip", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08sup3.zip" }, "related_objects": [ { "basename": "PUTnat08sup4.txt", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08sup4.txt" }, { "basename": "PUTnat08sup5.txt", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08sup5.txt" }, { "basename": "PUTnat08sup6.pdf", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08sup6.pdf" }, { "basename": "PUTnat08supp1.pdf", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08supp1.pdf" }, { "basename": "PUTnat08.pdf", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08.pdf" }, { "basename": "PUTnat08sup2.txt", "url": "https://authors.library.caltech.edu/records/qea1q-7ay26/files/PUTnat08sup2.txt" } ], "resource_type": "article", "pub_year": "2008", "author_list": "Putnam, Nicholas H.; Butts, Thomas; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tnkzv-v5s06", "eprint_id": 64129, "eprint_status": "archive", "datestamp": "2023-08-19 22:56:10", "lastmod": "2023-10-17 19:31:41", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Holland-L-Z", "name": { "family": "Holland", "given": "Linda Z." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Yu-Jr-Kai", "name": { "family": "Yu", "given": "Jr-Kai" } } ] }, "title": "The amphioxus genome illuminates vertebrate origins and cephalochordate biology", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2008 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). \n\nArticle published online before print. Received October 26, 2007; accepted in revised form February 24, 2008. Article and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.073676.107 \n\nThis work was funded by grants from the National Science Foundation, USA (L.Z.H.), National Institutes of Health, USA (G.W.L.), the Wellcome Trust (P.W.H.H.), BBSRC, UK (D.E.K.F., T.B., P.W.H.H.), and MEXT, Japan (H.S., N.S.), Center for Applied Genomics MSMT and Academy of Sciences, Czech Republic (Z.K.), and the 21st Century and Global COEs at Kyoto University (N. S.), from Ministerio de Educaci\u00f3n y Ciencia, Spain (J.G.-F.), MIUR Italy, FIRB 2001 BAU01WAFY (S.C., M.P.), JSPS, Japan (H.S.), and JSPS, Japan, CNRS and CRESCENDO, a European Union Integrated Project of FP6 (V.L., M.S.).\n\nPublished - 1100.full.pdf
Supplemental Material - Holland_Supplemental_references.doc
Supplemental Material - Holland_supplemental_figures-revised.pdf
Supplemental Material - Holland_supplemental_methods.doc
Supplemental Material - Holland_supplemental_tables-revised.pdf
", "abstract": "Cephalochordates, urochordates, and vertebrates evolved from a common ancestor over 520 million years ago. To improve our understanding of chordate evolution and the origin of vertebrates, we intensively searched for particular genes, gene families, and conserved noncoding elements in the sequenced genome of the cephalochordate Branchiostoma floridae, commonly called amphioxus or lancelets. Special attention was given to homeobox genes, opsin genes, genes involved in neural crest development, nuclear receptor genes, genes encoding components of the endocrine and immune systems, and conserved cis-regulatory enhancers. The amphioxus genome contains a basic set of chordate genes involved in development and cell signaling, including a fifteenth Hox gene. This set includes many genes that were co-opted in vertebrates for new roles in neural crest development and adaptive immunity. However, where amphioxus has a single gene, vertebrates often have two, three, or four paralogs derived from two whole-genome duplication events. In addition, several transcriptional enhancers are conserved between amphioxus and vertebrates\u2014a very wide phylogenetic distance. In contrast, urochordate genomes have lost many genes, including a diversity of homeobox families and genes involved in steroid hormone function. The amphioxus genome also exhibits derived features, including duplications of opsins and genes proposed to function in innate immunity and endocrine systems. Our results indicate that the amphioxus genome is elemental to an understanding of the biology and evolution of nonchordate deuterostomes, invertebrate chordates, and vertebrates.", "date": "2008-06-18", "date_type": "published", "publication": "Genome Research", "volume": "18", "number": "7", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "1100-1111", "id_number": "CaltechAUTHORS:20160201-113615215", "issn": "1088-9051", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160201-113615215", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF" }, { "agency": "NIH" }, { "agency": "Wellcome Trust" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)" }, { "agency": "Ministry of Education, Culture, Sports, Science and Technology (MEXT)" }, { "agency": "Center for Applied Genomics MSMT" }, { "agency": "Academy of Sciences, Czech Republic" }, { "agency": "21st Century and Global COEs" }, { "agency": "Ministerio de Educacion y Ciencia (MEC)" }, { "agency": "Ministero dell'Istruzione, dell'Universit\u00e0 e della Ricerca (MIUR)" }, { "agency": "FIRB 2001 BAU01WAFY" }, { "agency": "Japan Society for the Promotion of Science (JSPS)" }, { "agency": "CRESCENDO" }, { "agency": "European Union Integrated Project of FP6" }, { "agency": "Centre National de la Recherche Scientifique (CNRS)" } ] }, "doi": "10.1101/gr.073676.107", "pmcid": "PMC2493399", "primary_object": { "basename": "Holland_supplemental_tables-revised.pdf", "url": "https://authors.library.caltech.edu/records/tnkzv-v5s06/files/Holland_supplemental_tables-revised.pdf" }, "related_objects": [ { "basename": "1100.full.pdf", "url": "https://authors.library.caltech.edu/records/tnkzv-v5s06/files/1100.full.pdf" }, { "basename": "Holland_Supplemental_references.doc", "url": "https://authors.library.caltech.edu/records/tnkzv-v5s06/files/Holland_Supplemental_references.doc" }, { "basename": "Holland_supplemental_figures-revised.pdf", "url": "https://authors.library.caltech.edu/records/tnkzv-v5s06/files/Holland_supplemental_figures-revised.pdf" }, { "basename": "Holland_supplemental_methods.doc", "url": "https://authors.library.caltech.edu/records/tnkzv-v5s06/files/Holland_supplemental_methods.doc" } ], "resource_type": "article", "pub_year": "2008", "author_list": "Holland, Linda Z.; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hyhat-e2j90", "eprint_id": 64116, "eprint_status": "archive", "datestamp": "2023-08-19 22:50:45", "lastmod": "2023-10-17 19:31:10", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Insights From a Sea Lamprey Into the Evolution of Neural Crest Gene Regulatory Network", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2008 Marine Biological Laboratory. \n\nReceived 7 November 2007; accepted 11 March 2008.", "abstract": "The neural crest is a vertebrate innovation that forms at the embryonic neural plate border, transforms from epithelial to mesenchymal, migrates extensively throughout the embryo along well-defined pathways, and differentiates into a plethora of derivatives that include elements of peripheral nervous system, craniofacial skeleton, melanocytes, etc. The complex process of neural crest formation is guided by multiple regulatory modules that define neural crest gene regulatory network (NC GRN), which allows the neural crest to progressively acquire all of its defining characteristics. The molecular study of neural crest formation in lamprey, a basal extant vertebrate, consisting in identification and functional tests of molecular elements at each regulatory level of this network, has helped address the question of the timing of emergence of NC GRN and define its basal state. The results have revealed striking conservation in deployment of upstream factors and regulatory modules, suggesting that proximal portions of the network arose early in vertebrate evolution and have been tightly conserved for more than 500 million years. In contrast, certain differences were observed in deployment of some neural crest specifier and downstream effector genes expected to confer species-specific migratory and differentiation properties.", "date": "2008-06", "date_type": "published", "publication": "Biological Bulletin", "volume": "214", "number": "3", "publisher": "Marine Biological Laboratory", "pagerange": "303-314", "id_number": "CaltechAUTHORS:20160201-085800466", "issn": "0006-3185", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160201-085800466", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "article", "pub_year": "2008", "author_list": "Sauka-Spengler, Tatjana and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xsq6p-a1y29", "eprint_id": 11594, "eprint_status": "archive", "datestamp": "2023-08-22 11:46:44", "lastmod": "2023-10-17 15:09:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Essential role for PDGF signaling in ophthalmic trigeminal placode induction", "ispublished": "pub", "full_text_status": "public", "keywords": "PDGF, Induction, Neurogenesis, Trigeminal placode, Chicken", "note": "\u00a9 The Company of Biologists Ltd 2008. \n\nAccepted 20 March 2008. \n\nWe thank Samuel Ki and Matthew Jones for technical support, Dr Peter Lwigale for sharing unpublished data, and Drs Sujata Bhattacharyya and Laura Gammill for critical reading of the manuscript. This work was funded by NIH R01 DE16459.\n\nPublished - MCCdev08.pdf
", "abstract": "Much of the peripheral nervous system of the head is derived from ectodermal thickenings, called placodes, that delaminate or invaginate to form cranial ganglia and sense organs. The trigeminal ganglion, which arises lateral to the midbrain, forms via interactions between the neural tube and adjacent ectoderm. This induction triggers expression of Pax3, ingression of placode cells and their differentiation into neurons. However, the molecular nature of the underlying signals remains unknown. Here, we investigate the role of PDGF signaling in ophthalmic trigeminal placode induction. By in situ hybridization, PDGF receptor \u03b2 is expressed in the cranial ectoderm at the time of trigeminal placode formation, with the ligand PDGFD expressed in the midbrain neural folds. Blocking PDGF signaling in vitro results in a dose-dependent abrogation of Pax3 expression in recombinants of quail ectoderm with chick neural tube that recapitulate placode induction. In ovo microinjection of PDGF inhibitor causes a similar loss of Pax3 as well as the later placodal marker, CD151, and failure of neuronal differentiation. Conversely, microinjection of exogenous PDGFD increases the number of Pax3+ cells in the trigeminal placode and neurons in the condensing ganglia. Our results provide the first evidence for a signaling pathway involved in ophthalmic (opV) trigeminal placode induction.", "date": "2008-05-15", "date_type": "published", "publication": "Development", "volume": "135", "number": "10", "publisher": "Company of Biologists", "pagerange": "1863-1874", "id_number": "CaltechAUTHORS:MCCdev08", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:MCCdev08", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE16459" } ] }, "doi": "10.1242/dev.017954", "primary_object": { "basename": "MCCdev08.pdf", "url": "https://authors.library.caltech.edu/records/xsq6p-a1y29/files/MCCdev08.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "McCabe, Kathryn L. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/drk7k-pfj24", "eprint_id": 64476, "eprint_status": "archive", "datestamp": "2023-08-22 11:28:05", "lastmod": "2023-10-17 21:25:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Adams-M-S", "name": { "family": "Adams", "given": "Meghan S." } }, { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Discovery of transcription factors and other candidate regulators of neural crest development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest; transcription factors; chick; specification; migration; QPCR; EMT", "note": "\u00a9 2008 Wiley-Liss, Inc. \n\nAccepted 11 February 2008; article first published online: 19 Mar 2008. \n\nWe would like to thank Tatjana Sauka-Spengler and Lisa Taneyhill for technical guidance and the Bronner-Fraser lab for helpful discussions. This work was supported by grants from the NIH (DE015309 to L. S. G. and NS36585 to M. B. F.) as well as the Betty and Gordon Moore Fellowship (M. S. A.).", "abstract": "Neural crest cells migrate long distances and form divergent derivatives in vertebrate embryos. Despite previous efforts to identify genes up-regulated in neural crest populations, transcription factors have proved to be elusive due to relatively low expression levels and often transient expression. We screened newly induced neural crest cells for early target genes with the aim of identifying transcriptional regulators and other developmentally important genes. This yielded numerous candidate regulators, including 14 transcription factors, many of which were not previously associated with neural crest development. Quantitative real-time polymerase chain reaction confirmed up-regulation of several transcription factors in newly induced neural crest populations in vitro. In a secondary screen by in situ hybridization, we verified the expression of >100 genes in the neural crest. We note that several of the transcription factors and other genes from the screen are expressed in other migratory cell populations and have been implicated in diverse forms of cancer.", "date": "2008-04", "date_type": "published", "publication": "Developmental Dynamics", "volume": "237", "number": "4", "publisher": "Wiley-Liss, Inc.", "pagerange": "1021-1033", "id_number": "CaltechAUTHORS:20160212-120927931", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-120927931", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE015309" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "Gordon and Betty Moore Foundation" } ] }, "doi": "10.1002/dvdy.21513", "resource_type": "article", "pub_year": "2008", "author_list": "Adams, Meghan S.; Gammill, Laura S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xrzmf-jec63", "eprint_id": 64960, "eprint_status": "archive", "datestamp": "2023-08-19 22:16:52", "lastmod": "2023-10-17 22:01:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Shiau-Celia-E", "name": { "family": "Shiau", "given": "Celia E." }, "orcid": "0000-0002-9347-9158" }, { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } }, { "id": "Das-R-M", "name": { "family": "Das", "given": "Raman M." } }, { "id": "Wilson-S-A", "name": { "family": "Wilson", "given": "Stuart A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Robo2-Slit1 dependent cell-cell interactions mediate assembly of the trigeminal ganglion", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2008 Nature Publishing Group. \n\nReceived 19 November 2007; accepted 18 January 2008; published online 17 February 2008; corrected after print 24 February 2008.\n\nWe thank S. Guthrie for the Robo2D-GFP plasmid, members of the M.B.-F. lab for technical support, and S. Fraser, D. Meulemans and T. Hochgreb for comments on the manuscript. This work was supported by US National Institutes of Health (NIH) National Research Service Award 5T32 GM07616 to C.E.S., NIH Minority Supplement grant DE016459-07S1 to P.Y.L., a UK Biotechnology and Biological Sciences Research Council grant to R.M.D. and NIH grant DE16459 to M.B.-F. \n\nAuthor contributions: C.E.S. conducted all the experiments with help from P.Y.L. on the ablation and grafting experiments. C.E.S. designed the experiments and evaluated the data with contributions from P.Y.L. and M.B.-F. R.M.D. and S.A.W. designed and provided the RNAi reagents. C.E.S., P.Y.L. and M.B.-F. wrote the manuscript.\n\nSupplemental Material - nn2051-S1.pdf
", "abstract": "Vertebrate cranial sensory ganglia, responsible for sensation of touch, taste and pain in the face and viscera, are composed of both ectodermal placode and neural crest cells. The cellular and molecular interactions allowing generation of complex ganglia remain unknown. Here, we show that proper formation of the trigeminal ganglion, the largest of the cranial ganglia, relies on reciprocal interactions between placode and neural crest cells in chick, as removal of either population resulted in severe defects. We demonstrate that ingressing placode cells express the Robo2 receptor and early migrating cranial neural crest cells express its cognate ligand Slit1. Perturbation of this receptor-ligand interaction by blocking Robo2 function or depleting either Robo2 or Slit1 using RNA interference disrupted proper ganglion formation. The resultant disorganization mimics the effects of neural crest ablation. Thus, our data reveal a novel and essential role for Robo2-Slit1 signaling in mediating neural crest\u2013placode interactions during trigeminal gangliogenesis.", "date": "2008-03", "date_type": "published", "publication": "Nature Neuroscience", "volume": "11", "number": "3", "publisher": "Nature Publishing Group", "pagerange": "269-276", "id_number": "CaltechAUTHORS:20160302-102428489", "issn": "1097-6256", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-102428489", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5T32 GM07616" }, { "agency": "NIH", "grant_number": "DE016459-07S1" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)" }, { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1038/nn2051", "primary_object": { "basename": "nn2051-S1.pdf", "url": "https://authors.library.caltech.edu/records/xrzmf-jec63/files/nn2051-S1.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "Shiau, Celia E.; Lwigale, Peter Y.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/p583p-74z94", "eprint_id": 64950, "eprint_status": "archive", "datestamp": "2023-08-19 22:05:41", "lastmod": "2023-10-17 22:00:37", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja Jane" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Saving face: rescuing a craniofacial birth defect", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2008 Nature Publishing Group.", "abstract": "Craniofacial abnormalities comprise approximately one-third of all birth defects and often require multiple rounds of surgery to repair the malformations. To date, prevention has not been possible. Greater understanding of the developmental origin of craniofacial defects promises to provide insights into potential preventative measures.", "date": "2008-02", "date_type": "published", "publication": "Nature Medicine", "volume": "14", "number": "2", "publisher": "Nature Publishing Group", "pagerange": "115-116", "id_number": "CaltechAUTHORS:20160302-083319703", "issn": "1078-8956", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-083319703", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1038/nm0208-115", "resource_type": "article", "pub_year": "2008", "author_list": "McKeown, Sonja Jane and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/en0f2-6f078", "eprint_id": 40904, "eprint_status": "archive", "datestamp": "2023-08-19 21:50:36", "lastmod": "2023-10-24 22:34:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Chao-Jennifer-R", "name": { "family": "Chao", "given": "J. R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" }, { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "P. Y." } } ] }, "title": "Corneal Plasticity: Characterization of the Multipotentiality of Human Keratocytes", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2008 by the Association for Research in Vision and Ophthalmology, Inc. \n\nSupport: Fight for Sight Postdoctoral Fellowship Grant and Heed Fellowship to JRC, NIH grants to MBF, K99/R00 EY018050 to PYL.\n\nPublished - Bronner-Fraser_2008p4812.pdf
", "abstract": "Purpose: To determine the cell properties of adult human corneal keratocytes when\nchallenged in the chick embryonic environment.\nMethods: Cultured human keratocytes were injected along cranial neural crest\nmigratory pathways in chick embryos. Human keratocytes were also cultured under\nvarious conditions and differentiated into either fibroblasts or myofibroblasts, then\ntransplanted into the chick embryo. Migration of the injected cells was determined\nby immunohistochemistry using human cell-specific markers and markers of crest\nderivatives.\nResults: Injected human keratocytes proliferated and migrated ventrally adjacent\nto host neural crest cells. They contributed to numerous neural crest-derived tissues\nincluding cranial blood vessels, ocular tissues, musculature of the mandibular process,\nand cardiac cushion tissue.\nConclusions: Adult human corneal keratocytes that have undergone terminal\ndifferentiation can be induced to form cranial neural crest derivatives when grafted\ninto an embryonic environment.", "date": "2008", "date_type": "published", "publication": "ARVO Spring Meeting", "volume": "49", "publisher": "Association for Research in Vision and Ophthalmology", "pagerange": "4812", "id_number": "CaltechAUTHORS:20130822-083507699", "issn": "0273-0189", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130822-083507699", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Fight for Sight" }, { "agency": "Heed Fellowship" }, { "agency": "NIH", "grant_number": "K99/R00 EY018050" } ] }, "primary_object": { "basename": "Bronner-Fraser_2008p4812.pdf", "url": "https://authors.library.caltech.edu/records/en0f2-6f078/files/Bronner-Fraser_2008p4812.pdf" }, "resource_type": "article", "pub_year": "2008", "author_list": "Chao, J. R.; Bronner-Fraser, M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6qfce-j8591", "eprint_id": 63432, "eprint_status": "archive", "datestamp": "2023-08-19 21:52:18", "lastmod": "2024-01-13 16:31:10", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Garc\u00eda\u2010Castro-M", "name": { "family": "Garc\u00eda\u2010Castro", "given": "Mart\u00edn" } } ] }, "title": "Manipulations of Neural Crest Cells or Their Migratory Pathways", "ispublished": "unpub", "full_text_status": "restricted", "note": "\u00a9 2008 Elsevier Inc.", "abstract": "This chapter discusses techniques for the isolation, induction, and identification of neural crest cells in tissue culture as well as various manipulations of neural crest cells and some of the tissues with which they interact in the embryo. The formation of the embryo involves intricate cell movements, cell proliferation, and differentiation. The neural crest has long served as a model for the study of these processes because neural crest cells undergo extensive migrations and give rise to many diverse derivatives. Neural crest cells arise from the dorsal portion of the neural tube. Several unique properties of these cells make the neural crest an ideal system for studying cell migration and differentiation. First, these cells migrate extensively along characteristic pathways. Second, they give rise to diverse and numerous derivatives, ranging from pigment cells and cranial cartilage to adrenal chromaffin cells and the ganglia of the peripheral nervous system. Third, the characteristic position of premigratory neural crest cells within the dorsal portion of the neural tube makes them accessible to surgical and molecular manipulations during initial stages in their development. The methods described in this chapter provide a number of techniques that can be applied to the study of neural crest specification, migration, and differentiation.", "date": "2008", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "London", "pagerange": "75-96", "id_number": "CaltechAUTHORS:20160106-142628679", "isbn": "9780125641746", "book_title": "Avian Embryology, 2nd Edition", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160106-142628679", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" } } ] }, "doi": "10.1016/S0091-679X(08)00204-5", "resource_type": "book_section", "pub_year": "2008", "author_list": "Bronner-Fraser, Marianne and Garc\u00eda\u2010Castro, Mart\u00edn" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/chdw6-c0x73", "eprint_id": 40903, "eprint_status": "archive", "datestamp": "2023-08-19 21:50:31", "lastmod": "2023-10-24 22:34:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "P. Y." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Semaphorin3A Regulates Neural Crest Migration Into the Eye During Cornea Development", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2008 by the Association for Research in Vision and Ophthalmology, Inc.\n\nSupport: NIH Grant K99EY018050 to PYL and NIH Grants to MBF.", "abstract": "Purpose: To determine the role of lens-derived Semaphorin3A during neural crest\nmigration into the eye.\nMethods: Using in situ hybridization, we characterized the expression of Semaphorin3A\n(Sema3A) in the eye and its receptor Neuropilin-1 (Npn-1) by neural crest cells in the\nperiocular region during cornea development in chick embryos. Migration of neural\ncrest cells into the eye was tracked after lens ablation or inhibition of Sema3A in the\nlens using quail-chick chimera technique and immunohistochemistry.\nResults: We show that the lens continuously expresses Sema3A during cornea\ndevelopment and neural crest cells express Npn-1 in the periocular region. Interestingly,\nonly the neural crest cells that down regulate Npn-1 migrate into the eye to form the\ncornea. We also show that the lens, which immediately underlies the ectoderm,\ninhibits neural crest migration into the rudimentary eye since lensectomy results\nin premature migration and malformation of the cornea. Additionally, inhibiting of\nSema3A signaling in the lens phenocopies lensectomy.\nConclusions: Our results demonstrate that lens-derived Sema3A regulates periocular\nneural crest migration into the eye and is necessary for the proper formation of the\ncornea.", "date": "2008", "date_type": "published", "publication": "ARVO Spring Meeting", "volume": "49", "publisher": "Association for Research in Vision and Ophthalmology", "pagerange": "4809", "id_number": "CaltechAUTHORS:20130822-082729073", "issn": "0273-0189", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20130822-082729073", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "K99EY018050" } ] }, "resource_type": "article", "pub_year": "2008", "author_list": "Lwigale, P. Y. and Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mdfdp-xt693", "eprint_id": 63878, "eprint_status": "archive", "datestamp": "2023-08-22 10:44:05", "lastmod": "2023-10-17 17:15:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coles-E-G", "name": { "family": "Coles", "given": "E. G." } }, { "id": "Taneyhill-L-A", "name": { "family": "Taneyhill", "given": "L. A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A critical role for Cadherin6B in regulating avian neural crest emigration", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Cadherin; EMT; Delamination; Cell adhesion; (E)migration; Chick embryo", "note": "\u00a9 2007 Elsevier Inc. \n\nReceived for publication 24 May 2007; revised 27 September 2007; accepted 27 September 2007. Available online 5 October 2007. \n\nWe thank Dr. Stephen Price for the full-length Cad6B plasmid that was used as a PCR template and David Arce for excellent technical assistance. This work was supported by grants from the American Heart Association (0525037Y to EGC), NIH-NICHD (K99-HD055034 to LAT) and NIH (NS36585 to MBF).\n\nAccepted Version - nihms-36071.pdf
", "abstract": "Neural crest cells originate in the dorsal neural tube but subsequently undergo an epithelial-to-mesenchymal transition (EMT), delaminate, and migrate to diverse locations in the embryo where they contribute to a variety of derivatives. Cadherins are a family of cell\u2013cell adhesion molecules expressed in a broad range of embryonic tissues, including the neural tube. In particular, cadherin6B (Cad6B) is expressed in the dorsal neural tube prior to neural crest emigration but is then repressed by the transcription factor Snail2, expressed by premigratory and early migrating cranial neural crest cells. To examine the role of Cad6B during neural crest EMT, we have perturbed Cad6B protein levels in the cranial neural crest-forming region and have examined subsequent effects on emigration and migration. The results show that knock-down of Cad6B leads to premature neural crest cell emigration, whereas Cad6B overexpression disrupts migration. Our data reveal a novel role for Cad6B in controlling the proper timing of neural crest emigration and delamination from the neural tube of the avian embryo.", "date": "2007-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "312", "number": "2", "publisher": "Elsevier", "pagerange": "533-544", "id_number": "CaltechAUTHORS:20160122-095050832", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160122-095050832", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "0525037Y" }, { "agency": "National Institute of Child Health and Human Development (NICHD)", "grant_number": "K99-HD055034" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2007.09.056", "pmcid": "PMC2266065", "primary_object": { "basename": "nihms-36071.pdf", "url": "https://authors.library.caltech.edu/records/mdfdp-xt693/files/nihms-36071.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "Coles, E. G.; Taneyhill, L. A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g73fc-md982", "eprint_id": 13960, "eprint_status": "archive", "datestamp": "2023-08-22 10:13:58", "lastmod": "2023-10-18 15:59:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Spalt4 mediates invagination and otic placode gene expression in cranial ectoderm", "ispublished": "pub", "full_text_status": "public", "keywords": "SALL4; Sox10; Electroporation; Placode formation", "note": "\u00a9 Company of Biologists Ltd. \n\nAccepted 24 July 2007. \n\nWe thank Drs Tatjana Sauka-Spengler, Vivian Lee and Sujata Bhattacharyya for helpful comments on this manuscript. This work is supported by USPHS grant DE16459 to M.B.F.\n\nPublished - BARdev07.pdf
", "abstract": "Vertebrate placodes are regions of thickened head ectoderm that contribute to paired sensory organs and cranial ganglia. We demonstrate that the transcription factor Spalt4 (also known as Sall4) is broadly expressed in chick preplacodal epiblast and later resolves to otic, lens and olfactory placodes. Ectopic expression of Spalt4 by electroporation is sufficient to induce invagination of non-placodal head ectoderm and prevent neurogenic placodes from contributing to cranial ganglia. Conversely, loss of Spalt4 function in the otic placode results in abnormal otic vesicle development. Intriguingly, Spalt4 appears to initiate a placode program appropriate for the axial level but is not involved in later development of specific placode fates. Fgfs can regulate Spalt4, since implantation of Fgf2 beads into the area opaca induces its expression. The results suggest that Spalt4 is involved in early stages of placode development, initiating cranial ectodermal invagination and region-specific gene regulatory networks.", "date": "2007-10-12", "date_type": "published", "publication": "Development", "volume": "134", "number": "21", "publisher": "Company of Biologists Ltd", "pagerange": "3805-3814", "id_number": "CaltechAUTHORS:20090413-162216555", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090413-162216555", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" } ] }, "doi": "10.1242/dev.02885", "primary_object": { "basename": "BARdev07.pdf", "url": "https://authors.library.caltech.edu/records/g73fc-md982/files/BARdev07.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "Barembaum, Meyer and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1aqnk-16n96", "eprint_id": 64474, "eprint_status": "archive", "datestamp": "2023-08-22 10:09:36", "lastmod": "2023-10-17 21:25:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Shiau-Celia-E", "name": { "family": "Shiau", "given": "Celia E." }, "orcid": "0000-0002-9347-9158" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Identification of candidate secreted factors involved in trigeminal placode induction", "ispublished": "pub", "full_text_status": "restricted", "keywords": "trigeminal placode induction; FGF; IGF; PDGF; Shh; TGF\u03b2 superfamily; Wnt", "note": "\u00a9 2007 Wiley-Liss, Inc. \n\nAccepted 13 August 2007; published online 14 September 2007. \n\nFunded by NIH. Grant Number: R01 DE16459. \n\nWe thank Samuel Ki and Andrea Manzo for technical assistance.", "abstract": "Cranial ectodermal placodes are critical for normal development of the peripheral nervous system of the head. However, many aspects of the molecular and tissue interactions involved in their induction have yet to be elucidated. The trigeminal placode is induced by an unidentified secreted factor(s) from the dorsal neural tube. To determine candidates that may be involved in this induction process, we have performed reverse transcriptase-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization to screen for receptors expressed by uninduced presumptive trigeminal level ectoderm. We have found that receptors for fibroblast growth factors, insulin-like growth factors, platelet-derived growth factors, Sonic hedgehog, the transforming growth factor-beta superfamily, and Wnts all are expressed in patterns consistent with a role in trigeminal placode formation. This RT-PCR screen for candidate receptors expressed in presumptive trigeminal ectoderm is the first systematic screen to identify potential interactions underlying induction of the trigeminal placode and represents a critical step for understanding this complex process.", "date": "2007-10", "date_type": "published", "publication": "Developmental Dynamics", "volume": "236", "number": "10", "publisher": "Wiley-Liss, Inc.", "pagerange": "2925-2935", "id_number": "CaltechAUTHORS:20160212-105800028", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-105800028", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "R01 DE16459" } ] }, "doi": "10.1002/dvdy.21325", "resource_type": "article", "pub_year": "2007", "author_list": "McCabe, Kathryn L.; Shiau, Celia E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mz8x3-pk040", "eprint_id": 63889, "eprint_status": "archive", "datestamp": "2023-08-19 20:56:28", "lastmod": "2023-10-17 17:16:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Jones-M", "name": { "family": "Jones", "given": "Matthew" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ancient Evolutionary Origin of the Neural Crest Gene Regulatory Network", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2007 Elsevier Inc. \n\nReceived: December 14, 2006. Revised: March 9, 2007. Accepted: August 15, 2007. Published: September 4, 2007. \n\nWe thank Dr. J. Langeland for providing material and expertise during cDNA library construction; C. Krontiris and P. Fraser for excellent assistance; Drs. S. Fraser and N. Nikitina for critical reading of the manuscript; Dr. Y. Kee for sharing reagents and expertise; Drs. S. Bhattacharyya and M. Barembaum for helpful discussions; Drs. A. Monsoro-Burq and J.-P. Saint-Jeannet for providing Xenopus Msx1 and Zic1 clones; R. Bergstedt and the staff at Hammond Bay Biological Station for kindly providing animals; and N. Johnson for sending spawning adult lampreys. This work was supported by DE17911 to M.B.F.", "abstract": "The vertebrate neural crest migrates from its origin, the neural plate border, to form diverse derivatives. We previously hypothesized that a neural crest gene regulatory network (NC-GRN) guides neural crest formation. Here, we investigate when during evolution this hypothetical network emerged by analyzing neural crest formation in lamprey, a basal extant vertebrate. We identify 50 NC-GRN homologs and use morpholinos to demonstrate a critical role for eight transcriptional regulators. The results reveal conservation in deployment of upstream factors, suggesting that proximal portions of the network arose early in vertebrate evolution and have been conserved for >500 million years. We found biphasic expression of neural crest specifiers and differences in deployment of some specifiers and effectors expected to confer species-specific properties. By testing the collective expression and function of neural crest genes in a single, basal vertebrate, we reveal the ground state of the NC-GRN and resolve ambiguities between model organisms.", "date": "2007-09-07", "date_type": "published", "publication": "Developmental Cell", "volume": "13", "number": "3", "publisher": "Cell Press", "pagerange": "405-420", "id_number": "CaltechAUTHORS:20160122-105354645", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160122-105354645", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE17911" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.devcel.2007.08.005", "resource_type": "article", "pub_year": "2007", "author_list": "Sauka-Spengler, Tatjana; Meulemans, Daniel; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ewnz5-njn96", "eprint_id": 9950, "eprint_status": "archive", "datestamp": "2023-08-22 09:49:57", "lastmod": "2023-10-16 22:35:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Insights from Amphioxus into the Evolution of Vertebrate Cartilage", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2007 Meulemans, Bronner-Fraser. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. \n\nReceived: May 9, 2007; Accepted: August 1, 2007; Published: August 29, 2007. Academic Editor: Jean-Nicolas Volff, Ecole Normale Sup\u00e9rieure de Lyon, France. \n\nPhage library was a gift from J. Langeland. Thanks to J.K. Yu for pre-publication access to the amphioxus EST database, J.K. Yu and L.Z. Holland for EST library clones, J. Lawrence for facilities in Tampa, Florida, and the Joint Genome Institute for access to Amphioxus Genome Release v1.0. \n\nAuthor Contributions: Conceived and designed the experiments: DM. Performed the experiments: DM. Analyzed the data: DM. Contributed reagents/materials/analysis tools: DM. Wrote the paper: MB DM. \n\nFunding: Funding for this work was provided by NIH grant DE017911 to MBF. \n\nCompeting interests: The authors have declared that no competing interests exist.\n\nPublished - MEUplosone07.pdf
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Supplemental Material - MEUplosone07supp.pdf
", "abstract": "Central to the story of vertebrate evolution is the origin of the vertebrate head, a problem difficult to approach using paleontology and comparative morphology due to a lack of unambiguous intermediate forms. Embryologically, much of the vertebrate head is derived from two ectodermal tissues, the neural crest and cranial placodes. Recent work in protochordates suggests the first chordates possessed migratory neural tube cells with some features of neural crest cells. However, it is unclear how and when these cells acquired the ability to form cellular cartilage, a cell type unique to vertebrates. It has been variously proposed that the neural crest acquired chondrogenic ability by recruiting proto-chondrogenic gene programs deployed in the neural tube, pharynx, and notochord. To test these hypotheses we examined the expression of 11 amphioxus orthologs of genes involved in neural crest chondrogenesis. Consistent with cellular cartilage as a vertebrate novelty, we find that no single amphioxus tissue co-expresses all or most of these genes. However, most are variously co-expressed in mesodermal derivatives. Our results suggest that neural crest-derived cartilage evolved by serial cooption of genes which functioned primitively in mesoderm.", "date": "2007-08-29", "date_type": "published", "publication": "PLoS ONE", "volume": "2", "number": "8", "publisher": "PLoS ONE", "pagerange": "Art. No. e787", "id_number": "CaltechAUTHORS:MEUplosone07", "issn": "1932-6203", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:MEUplosone07", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" } ] }, "doi": "10.1371/journal.pone.0000787", "pmcid": "PMC1950077", "primary_object": { "basename": "MEUplosone07figS1.tif", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07figS1.tif" }, "related_objects": [ { "basename": "MEUplosone07figS4.tif", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07figS4.tif" }, { "basename": "MEUplosone07supp.pdf", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07supp.pdf" }, { "basename": "MEUplosone07.pdf", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07.pdf" }, { "basename": "MEUplosone07figS2.tif", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07figS2.tif" }, { "basename": "MEUplosone07figS3.tif", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07figS3.tif" }, { "basename": "MEUplosone07figS5.tif", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/MEUplosone07figS5.tif" }, { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/medium.png" }, { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/ewnz5-njn96/files/small.png" } ], "resource_type": "article", "pub_year": "2007", "author_list": "Meulemans, Daniel and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w2f2d-0m533", "eprint_id": 11843, "eprint_status": "archive", "datestamp": "2023-08-22 09:43:32", "lastmod": "2023-10-17 15:49:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The Amphioxus SoxB Family: Implications for the Evolution of Vertebrate Placodes", "ispublished": "pub", "full_text_status": "public", "keywords": "Evolution, development, chordates, vertebrates, placodes, amphioxus", "note": "Copyright \u00a92008 Ivyspring International Publisher. \n\nReceived 2007-5-21. Accepted 2007-8-5. Published 2007-8-6. \n\nPhage library was a gift from J. Langeland. Thanks to J.K. Yu for pre-publication access to the amphioxus EST database, J.K. Yu and L.Z. Holland for EST library clones, J. Lawrence for facilities in Tampa, Florida, and the Joint Genome Institute for access to Amphioxus Genome Release v1.0. Funding for this work was provided by NIH grant DE017911 to MBF. \n\nThe authors have declared that no conflict of interest exists.\n\nPublished - MEUijbs07.pdf
", "abstract": "Cranial placodes are regions of thickened ectoderm that give rise to sense organs and ganglia in the vertebrate head. Homologous structures are proposed to exist in urochordates, but have not been found in cephalochordates, suggesting the first chordates lacked placodes. SoxB genes are expressed in discrete subsets of vertebrate placodes. To investigate how placodes arose and diversified in the vertebrate lineage we isolated the complete set of SoxB genes from amphioxus and analyzed their expression in embryos and larvae. We find that while amphioxus possesses a single SoxB2 gene, it has three SoxB1 paralogs. Like vertebrate SoxB1 genes, one of these paralogs is expressed in non-neural ectoderm destined to give rise to sensory cells. When considered in the context of other amphioxus placode marker orthologs, amphioxus SoxB1 expression suggests a diversity of sensory cell types utilizing distinct placode-type gene programs was present in the first chordates. Our data supports a model for placode evolution and diversification whereby the full complement of vertebrate placodes evolved by serial recruitment of distinct sensory cell specification programs to anterior pre-placodal ectoderm.", "date": "2007-08-06", "date_type": "published", "publication": "International Journal of Biosciences", "volume": "3", "number": "6", "publisher": "Ivyspring International Publisher", "pagerange": "356-364", "id_number": "CaltechAUTHORS:MEUijbs07", "issn": "1449-2288", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:MEUijbs07", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE017911" } ] }, "pmcid": "PMC1950271", "primary_object": { "basename": "MEUijbs07.pdf", "url": "https://authors.library.caltech.edu/records/w2f2d-0m533/files/MEUijbs07.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "Meulemans, Daniel and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9xkhs-7zh50", "eprint_id": 64885, "eprint_status": "archive", "datestamp": "2023-08-22 09:40:20", "lastmod": "2023-10-17 21:57:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "de-Bellard-Maria-Elena", "name": { "family": "De Bellard", "given": "Maria Elena" }, "orcid": "0000-0001-9881-0447" }, { "id": "Barembaum-Meyer", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Arman-Odette", "name": { "family": "Arman", "given": "Odette" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Lunatic fringe causes expansion and increased neurogenesis of trunk neural tube and neural crest populations", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; lunatic fringe; notch; cell division", "note": "\u00a9 2007 Cambridge University Press. \n\nReceived November 14 2007. Accepted November 16 2007. Published online: 06 February 2008. \n\nSpecial thanks to Gustavo Gomez and Ruel Velazco for their expert technical assistance and to Cindy Malone for help editing this manuscript. We thank Vivian Lee, Yun Kee and Christine Nelleman for useful discussions and Ed Laufer for providing the RCAS virus used in these experiments. This work was supported in part by a postdoctoral fellowship to MEdB from the National Multiple Sclerosis Society (FA 1383-A-1) and NIH-MBRS SCORE-5S06GM048680-13; and by a USPHS NS36585 and DE13223 to MBF.\n\nAccepted Version - nihms39277.pdf
", "abstract": "Both neurons and glia of the PNS are derived from the neural crest. In this study, we have examined the potential function of lunatic fringe in neural tube and trunk neural crest development by gain-of-function analysis during early stages of nervous system formation. Normally lunatic fringe is expressed in three broad bands within the neural tube, and is most prominent in the dorsal neural tube containing neural crest precursors. Using retrovirally-mediated gene transfer, we find that excess lunatic fringe in the neural tube increases the numbers of neural crest cells in the migratory stream via an apparent increase in cell proliferation. In addition, lunatic fringe augments the numbers of neurons and upregulates Delta-1 expression. The results indicate that, by modulating Notch/Delta signaling, lunatic fringe not only increases cell division of neural crest precursors, but also increases the numbers of neurons in the trunk neural crest.", "date": "2007-08", "date_type": "published", "publication": "Neuron Glia Biology", "volume": "3", "number": "2", "publisher": "Cambridge University Press", "pagerange": "93-103", "id_number": "CaltechAUTHORS:20160301-070210885", "issn": "1740-925X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160301-070210885", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Multiple Sclerosis Society", "grant_number": "FA 1383-A-1" }, { "agency": "NIH", "grant_number": "SCORE-5S06GM048680-13" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "DE13223" } ] }, "doi": "10.1017/S1740925X07000683", "pmcid": "PMC2293300", "primary_object": { "basename": "nihms39277.pdf", "url": "https://authors.library.caltech.edu/records/9xkhs-7zh50/files/nihms39277.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "De Bellard, Maria Elena; Barembaum, Meyer; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xwc1t-j8519", "eprint_id": 63849, "eprint_status": "archive", "datestamp": "2023-08-22 09:20:27", "lastmod": "2023-10-17 17:13:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Lens-derived Semaphorin3A regulates sensory innervation of the cornea", "ispublished": "pub", "full_text_status": "public", "keywords": "Cornea; Lens; Trigeminal sensory nerves; Semaphorin3A; Neuropilin-1", "note": "\u00a9 2007 Elsevier Inc. \n\nReceived for publication 11 January 2007; revised 6 April 2007; accepted 11 April 2007. Available online 18 April 2007. \n\nWe thank Drs. S. E. Fraser and L. S. Gammill for the helpful comments on the manuscript. This work was supported in part by a CALTECH Elizabeth Ross Fellowship and NIH K99/R00 grant EY018050 (to PYL) and NIH grant R01DE16459 to MBF.\n\nSupplemental Material - mmc1.pdf
", "abstract": "The cornea, one of the most highly innervated tissues of the body, is innervated by trigeminal sensory afferents. During development, axons are initially repelled at the corneal margin, resulting in the formation of a circumferential nerve ring. The nature and source of guidance molecules that regulate this process remain a mystery. Here, we show that the lens, which immediately underlies the cornea, repels trigeminal axons in vivo and in vitro. Lens ablation results in premature, disorganized corneal innervation and disruption of the nerve ring and ventral plexus. We show that Semaphorin3A (Sema3A) is expressed in the lens epithelium and its receptor Neuropilin-1 (Npn1) is expressed in the trigeminal ganglion during cornea development. Inhibition of Sema3A signaling abrogates axon repulsion by the lens and cornea in vitro and phenocopies lens removal in vivo. These results demonstrate that lens-derived Sema3A mediates initial repulsion of trigeminal sensory axons from the cornea and is necessary for the proper formation of the nerve ring and positioning of the ventral plexus in the choroid fissure.", "date": "2007-06-15", "date_type": "published", "publication": "Developmental Biology", "volume": "306", "number": "2", "publisher": "Elsevier", "pagerange": "750-759", "id_number": "CaltechAUTHORS:20160121-135401314", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-135401314", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Elizabeth Ross Fellowship" }, { "agency": "NIH", "grant_number": "K99/R00 EY018050" }, { "agency": "NIH", "grant_number": "R01DE16459" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2007.04.012", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/xwc1t-j8519/files/mmc1.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "Lwigale, Peter Y. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8jw7k-ep021", "eprint_id": 64859, "eprint_status": "archive", "datestamp": "2023-08-19 20:20:27", "lastmod": "2023-10-17 21:56:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Trinh-Le-A", "name": { "family": "Trinh", "given": "Le A." } }, { "id": "McCutchen-M", "name": { "family": "McCutchen", "given": "Marshall" } }, { "id": "Bronner-M-E", "name": { "family": "Bonner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bumm-L", "name": { "family": "Bumm", "given": "Lloyd" } }, { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David" } } ] }, "title": "Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2007 BioTechniques. \n\nReceived 5 March 2007; accepted 6 April 2007. \n\nL.A.T., M.D.M., L.A.B., and D.W.M. contributed equally to this work. \n\nM.D.M. thanks the National Science Foundation (NSF) Louis Stokes Oklahoma Alliance for Minority Participation, Bridges to the Doctorate Program for a graduate research fellowship. This work was supported by the NSF CAREER grant no. CHE-0239803, the Center for Physics in Nanostructures, NSF MRSEC no. DMR-0080054, Oklahoma EPSCoR, and NIH grants no. R01 HL078694 and P01 HD037105. \n\nThe authors declare no competing interests.", "abstract": "In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant fluorescence that enables the use of confocal microscopy to generate high-resolution three-dimensional (3-D) imaging of gene expression.", "date": "2007-06", "date_type": "published", "publication": "BioTechniques", "volume": "42", "number": "6", "publisher": "Informa Healthcare", "pagerange": "756-759", "id_number": "CaltechAUTHORS:20160229-142228899", "issn": "0736-6205", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160229-142228899", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF Graduate Research Fellowship" }, { "agency": "NSF", "grant_number": "CHE-0239803" }, { "agency": "Oklahoma Experimental Program to Stimulate Competitive Research (EPSCoR)" }, { "agency": "NSF", "grant_number": "DMR-0080054" }, { "agency": "NIH", "grant_number": "R01 HL078694" }, { "agency": "NIH", "grant_number": "P01 HD037105" } ] }, "doi": "10.2144/000112476", "resource_type": "article", "pub_year": "2007", "author_list": "Trinh, Le A.; McCutchen, Marshall; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0scpg-jtj69", "eprint_id": 16878, "eprint_status": "archive", "datestamp": "2023-08-22 08:53:40", "lastmod": "2023-10-19 22:37:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Taneyhill-L-A", "name": { "family": "Taneyhill", "given": "Lisa A." } }, { "id": "Coles-E-G", "name": { "family": "Coles", "given": "Edward G." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Snail2 directly represses cadherin6B during epithelial-to-mesenchymal transitions of the neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Snail2 (Slug); cadherin6B; Neural crest; Epithelial-to-mesenchymal transitions; E boxes", "note": "\u00a9 2007 Company of Biologists Ltd.\n\nAccepted 5 February 2007; first published online 7 March 2007.\n\nWe thank Tatjana Sauka-Spengler and Titus Brown for assistance with genomic analysis, Peter Farlie for help with the Snail2 immunohistochemistry protocol, Dorota Skowronska-Krawczyk for advice on the chromatin\nimmunoprecipitations, and David Arce for excellent technical assistance. This work was supported by grants from the NIH-NICHD (NRSA-F32 HD43535 to L.A.T.), American Heart Association (0525037Y to E.G.C.) and NIH (NS36585 to M.B.-F.).\n\nPublished - TANdev07.pdf
Supplemental Material - DEV02834figS1.jpg
Supplemental Material - DEV02834figS2.jpg
Supplemental Material - DEV02834figS3.jpg
Supplemental Material - DEV02834figS4.jpg
", "abstract": "The neural crest, a transient population of migratory cells, forms the craniofacial skeleton and peripheral nervous system, among other derivatives in vertebrate embryos. The transcriptional repressor Snail2 is thought to be crucial for the epithelial-to-mesenchymal transition (EMT) that promotes neural crest delamination from the neural tube; however, little is known about its downstream targets. To this end, we depleted avian Snail2 in the premigratory neural crest using morpholino antisense oligonucleotides and examined effects on potential targets by quantitative PCR. Several dorsal neural tube genes were upregulated by alleviating Snail2 repression; moreover, the cell adhesion molecule cadherin6B was derepressed within 30 minutes of blocking Snail2 translation. Examination of the chick cadherin6B genomic sequence reveals that the regulatory region contains three pairs of clustered E boxes, representing putative Snail2 binding sites. Furthermore, in vivo and in vitro biochemical analyses demonstrate that Snail2 directly binds to these sites and regulates cadherin6B transcription. These results are the first to describe a direct target of Snail2 repression in vivo and in the context of the EMT that characterizes neural crest development", "date": "2007-04-15", "date_type": "published", "publication": "Development", "volume": "134", "number": "8", "publisher": "Company of Biologists", "pagerange": "1481-1490", "id_number": "CaltechAUTHORS:20091204-110412097", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20091204-110412097", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Institute of Child Health and Human Development (NICHD)", "grant_number": "NRSA-F32 HD43535" }, { "agency": "American Heart Association", "grant_number": "0525037Y" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1242/dev.02834", "primary_object": { "basename": "DEV02834figS1.jpg", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/DEV02834figS1.jpg" }, "related_objects": [ { "basename": "DEV02834figS2.jpg", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/DEV02834figS2.jpg" }, { "basename": "DEV02834figS3.jpg", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/DEV02834figS3.jpg" }, { "basename": "DEV02834figS4.jpg", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/DEV02834figS4.jpg" }, { "basename": "TANdev07.pdf", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/TANdev07.pdf" }, { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/medium.png" }, { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/0scpg-jtj69/files/small.png" } ], "resource_type": "article", "pub_year": "2007", "author_list": "Taneyhill, Lisa A.; Coles, Edward G.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wkfr0-csh30", "eprint_id": 64115, "eprint_status": "archive", "datestamp": "2023-08-19 19:41:53", "lastmod": "2023-10-17 19:31:05", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Hwang-Byung-Joon", "name": { "family": "Hwang", "given": "Byung Joon" } }, { "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "orcid": "0000-0002-7699-0173" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest, cell migration, C. elegans HSN, comparative genomic analysis", "note": "\u00a9 2007 by Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). \n\nReceived November 7, 2006; revised version accepted January 9, 2007.\n\nWe thank Samuel Ki for technical assistance and colleagues in the M.B.-F. laboratory for technical advice and discussion. We especially thank Drs. Titus Brown, Scott Fraser, Mihoko Kato, Daniel Meulemans, Ellen Rothenberg, Tatjana Sauka-Spengler, Erich Schwarz, and J.K. Sky\nYu for helpful discussions on the manuscript, and Drs. Dominique Alfandari, Tamara Allison, Rik Derynck, Todd Evans, Cees Oudejans, and Richard Harland for sharing the constructs. This work was supported by NIH grants NS36585 and NS051051 (to M.B.-F.); by the Howard Hughes Medical Institute, of which P.W.S. is an Investigator; and by\nNHGRI Genome Scholar Development and Faculty Transition Award (K22) (to B.J.H.).\n\nPublished - 391.full.pdf
Supplemental Material - KeeSuppMat.doc
", "abstract": "Because migratory cells in all animals share common properties, we hypothesized that genetic networks involved in cell migration may be conserved between nematodes and vertebrates. To explore this, we performed comparative genomic analysis to identify vertebrate orthologs of genes required for hermaphrodite-specific neuron (HSN) migration in Caenoryhabditis elegans, and then examined their expression and function in the vertebrate neural crest. The results demonstrate high conservation of regulatory components involved in long-range migrations across diverse species. Although the neural crest is a vertebrate innovation, the results suggest that its migratory properties evolved by utilizing programs already present in the common vertebrate\u2013invertebrate ancestor.", "date": "2007-02-15", "date_type": "published", "publication": "Genes and Development", "volume": "21", "number": "4", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "391-396", "id_number": "CaltechAUTHORS:20160201-085752814", "issn": "0890-9369", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160201-085752814", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS051051" }, { "agency": "Howard Hughes Medical Institute (HHMI)" }, { "agency": "National Human Genome Research Institute (NHGRI)" }, { "agency": "Faculty Transition Award", "grant_number": "K22" } ] }, "doi": "10.1101/gad.1509307", "pmcid": "PMC1804327", "primary_object": { "basename": "KeeSuppMat.doc", "url": "https://authors.library.caltech.edu/records/wkfr0-csh30/files/KeeSuppMat.doc" }, "related_objects": [ { "basename": "391.full.pdf", "url": "https://authors.library.caltech.edu/records/wkfr0-csh30/files/391.full.pdf" } ], "resource_type": "article", "pub_year": "2007", "author_list": "Kee, Yun; Hwang, Byung Joon; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9xgx6-evt50", "eprint_id": 55877, "eprint_status": "archive", "datestamp": "2023-08-19 19:39:10", "lastmod": "2023-10-20 23:21:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Yu-Jr-Kai", "name": { "family": "Yu", "given": "Jr-Kai" } }, { "id": "Satou-Yutaka", "name": { "family": "Satou", "given": "Yutaka" } }, { "id": "Holland-N-D", "name": { "family": "Holland", "given": "Nicholas D." } }, { "id": "Shin-I-Tadasu", "name": { "family": "Shin-I", "given": "Tadasu" } }, { "id": "Kohara-Yuji", "name": { "family": "Kohara", "given": "Yuji" } }, { "id": "Satoh-Noriyuki", "name": { "family": "Satoh", "given": "Noriyuki" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Holland-L-Z", "name": { "family": "Holland", "given": "Linda Z." } } ] }, "title": "Axial patterning in cephalochordates and the evolution of the organizer", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2007 Macmillan Publishers Limited.\n\nReceived 29 May; accepted 20 November 2006; Published online 21 January 2007.\n\nWe are indebted to J.M. Lawrence, University of South Florida, for providing laboratory facilities during the summer breeding season of amphioxus. This work was funded by grants from the National Science Foundation, USA (L.Z.H. and N.D.H.), the National Aeronautics and Space Administration, USA (M.B.-F. and L.Z.H.), the National Institutes of Health (M.B.-F.), MEXT, Japan (N.S. and Y.K.), and the 21st Century COE for the Biodiversity Research at Kyoto University (N.S.). J.-K.Y is currently supported by the Della Martin prize postdoctoral fellowship from the Division of Biology, California Institute of\nTechnology, USA.\nEST sequences were deposited in the DDBJ/EMBL/GenBank databases. (DNA Data Bank of Japan accession numbers BW692960\u2013BW954996). \n\nThe authors declare no competing financial interests. Correspondence and requests for materials should be addressed to L.Z.H. (lzholland@ucsd.edu).\n\nSupplemental Material - nature05472-s1.pdf
", "abstract": "The organizer of the vertebrate gastrula is an important signalling centre that induces and patterns dorsal axial structures. Although a topic of long-standing interest, the evolutionary origin of the organizer remains unclear. Here we show that the gastrula of the cephalochordate amphioxus expresses dorsal/ventral (D/V) patterning genes (for example, bone morphogenetic proteins (BMPs), Nodal and their antagonists) in patterns reminiscent of those of their vertebrate orthlogues, and that amphioxus embryos, like those of vertebrates, are ventralized by exogenous BMP protein. In addition, Wnt-antagonists (for example, Dkks and sFRP2-like) are expressed anteriorly, whereas Wnt genes themselves are expressed posteriorly, consistent with a role for Wnt signalling in anterior/posterior (A/P) patterning. These results suggest evolutionary conservation of the mechanisms for both D/V and A/P patterning of the early gastrula. In light of recent phylogenetic analyses placing cephalochordates basally in the chordate lineage, we propose that separate signalling centres for patterning the D/V and A/P axes may be an ancestral chordate character.", "date": "2007-02-08", "date_type": "published", "publication": "Nature", "volume": "445", "number": "7128", "publisher": "Nature Publishing Group", "pagerange": "613-617", "id_number": "CaltechAUTHORS:20150318-085021198", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150318-085021198", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF" }, { "agency": "NASA" }, { "agency": "NIH" }, { "agency": "Japan Society for the Promotion of Science (JSPS)" }, { "agency": "Ministry of Education, Culture, Sports, Science and Technology (MEXT)" }, { "agency": "Kyoto University 21st Century COE for the Biodiversity Research" }, { "agency": "Caltech Della Martin prize postdoctoral fellowship" } ] }, "doi": "10.1038/nature05472", "primary_object": { "basename": "nature05472-s1.pdf", "url": "https://authors.library.caltech.edu/records/9xgx6-evt50/files/nature05472-s1.pdf" }, "resource_type": "article", "pub_year": "2007", "author_list": "Yu, Jr-Kai; Satou, Yutaka; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2cdn1-5xq14", "eprint_id": 64466, "eprint_status": "archive", "datestamp": "2023-08-22 08:20:08", "lastmod": "2023-10-17 21:25:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Epperlein-H-H", "name": { "family": "Epperlein", "given": "Hans-Henning" } }, { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Mchedlishvili-L", "name": { "family": "Mchedlishvili", "given": "Levan" } }, { "id": "Cerny-R", "name": { "family": "Cerny", "given": "Robert" } }, { "id": "Sobkow-L", "name": { "family": "Sobkow", "given": "Lidia" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Migratory Patterns and Developmental Potential of Trunk Neural Crest Cells in the Axolotl Embryo", "ispublished": "pub", "full_text_status": "restricted", "keywords": "DiI; fluorescent dextrans; GFP transgenic embryos; trunk neural crest; migration; dorsal fin; Rohon-Beard cells; lateral line; axolotl", "note": "\u00a9 2006 Wiley-Liss, Inc. \n\nAccepted 31 October 2006. Published online 19 December 2006. Article first published online: 20 Dec 2006. \n\nThis research was supported by DFG (EP8/7-1) to H.H.E., by NS051051 to M.B.F., and a James H. Zumberge Research and Innovation Fund and a Donald E. and Delia B. Baxter Foundation award to M.A.J.S. H.H.E. thanks G. Northcutt (San Diego) and G. Schlosser (Bremen) for valuable information on the lateral line, I. Beck, P. Mirtschink, F. Heidrich, and T. Schwalm for analysis of cell migration or computer work, and S. Bramke for histology.", "abstract": "Using cell markers and grafting, we examined the timing of migration and developmental potential of trunk neural crest cells in axolotl. No obvious differences in pathway choice were noted for DiI-labeling at different lateral or medial positions of the trunk neural folds in neurulae, which contributed not only to neural crest but also to Rohon-Beard neurons. Labeling wild-type dorsal trunks at pre- and early-migratory stages revealed that individual neural crest cells migrate away from the neural tube along two main routes: first, dorsolaterally between the epidermis and somites and, later, ventromedially between the somites and neural tube/notochord. Dorsolaterally migrating crest primarily forms pigment cells, with those from anterior (but not mid or posterior) trunk neural folds also contributing glia and neurons to the lateral line. White mutants have impaired dorsolateral but normal ventromedial migration. At late migratory stages, most labeled cells move along the ventromedial pathway or into the dorsal fin. Contrasting with other anamniotes, axolotl has a minor neural crest contribution to the dorsal fin, most of which arises from the dermomyotome. Taken together, the results reveal stereotypic migration and differentiation of neural crest cells in axolotl that differ from other vertebrates in timing of entry onto the dorsolateral pathway and extent of contribution to some derivatives.", "date": "2007-02", "date_type": "published", "publication": "Developmental Dynamics", "volume": "236", "number": "2", "publisher": "Wiley-Liss, Inc.", "pagerange": "389-403", "id_number": "CaltechAUTHORS:20160212-100711722", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-100711722", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "Ep8/7-1" }, { "agency": "NIH", "grant_number": "NS051051" }, { "agency": "James H. Zumberge Research and Innovation Fund" }, { "agency": "Donald E. and Delia B. Baxter Foundation" } ] }, "doi": "10.1002/dvdy.21039", "resource_type": "article", "pub_year": "2007", "author_list": "Epperlein, Hans-Henning; Selleck, Mark A. J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mgjr0-vg021", "eprint_id": 64481, "eprint_status": "archive", "datestamp": "2023-08-22 08:02:33", "lastmod": "2023-10-17 21:26:19", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Gonzales-C", "name": { "family": "Gonzalez", "given": "Constanza" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neuropilin 2/Semaphorin 3F Signaling is Essential for Cranial Neural Crest Migration and Trigeminal Ganglion Condensation", "ispublished": "pub", "full_text_status": "restricted", "keywords": "cranial neural crest migration; trigeminal ganglia; neuropilin 2; semaphorin 3F", "note": "\u00a9 2006 Wiley Periodicals, Inc. \n\nReceived 25 April 2006; revised 12 July 2006; accepted 17 July 2006. Article first published online: 22 Dec 2006. \n\nUSPHS. Grant Numbers: DE15309, NS051051. \n\nWe are extremely grateful to David Ginty and Chenghua Gu for providing the npn2 and sema3F in situ probes, the npn2 knockout mice and sema3F mutant embryos, as well as helpful comments throughout the course of this work. Special thanks to Joaquin Gutierrez for exceptional animal care and to members of the Bronner-Fraser laboratory for helpful discussions.", "abstract": "In the head of vertebrate embryos, neural crest cells migrate from the neural tube into the presumptive facial region and condense to form cranial ganglia and skeletal elements in the branchial arches. We show that newly formed neural folds and migrating neural crest cells express the neuropilin 2 (npn2) receptor in a manner that is highly conserved in amniotes. The repulsive npn2 ligand semaphorin (sema) 3F is expressed in a complementary pattern in the mouse. Furthermore, mice carrying null mutations for either npn2 or sema3F have abnormal cranial neural crest migration. Most notably, \"bridges\" of migrating cells are observed crossing between neural crest streams entering branchial arches 1 and 2. In addition, trigeminal ganglia fail to form correctly in the mutants and are improperly condensed and loosely organized. These data show that npn2/sema3F signaling is required for proper cranial neural crest development in the head.", "date": "2007-01", "date_type": "published", "publication": "Journal of Neurobiology", "volume": "67", "number": "1", "publisher": "Wiley", "pagerange": "47-56", "id_number": "CaltechAUTHORS:20160212-144858627", "issn": "0022-3034", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-144858627", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE15309" }, { "agency": "NIH", "grant_number": "NS051051" } ] }, "doi": "10.1002/dneu.20326", "resource_type": "article", "pub_year": "2007", "author_list": "Gammill, Laura S.; Gonzalez, Constanza; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/k0sna-h0320", "eprint_id": 64100, "eprint_status": "archive", "datestamp": "2023-08-19 18:41:38", "lastmod": "2023-10-17 19:30:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bailey-A-P", "name": { "family": "Bailey", "given": "Andrew P." } }, { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Streit-A", "name": { "family": "Streit", "given": "Andrea" }, "orcid": "0000-0001-7664-7917" } ] }, "title": "Lens Specification Is the Ground State of All Sensory Placodes, from which FGF Promotes Olfactory Identity", "ispublished": "pub", "full_text_status": "public", "keywords": "DEVBIO", "note": "\u00a9 Received: April 7, 2006. \n\nRevised: July 15, 2006. Accepted: August 18, 2006. Published: October 2, 2006. \n\nWe thank Sharon Pudaruth for excellent technical assistance and Claudio Stern for critical comments on the manuscript. This work was funded by grant USPHS DE16459 to M.B.-F., and by a Fight for Sight studentship and a BBSRC project grant (D010659/1) to A.S.\n\nSupplemental Material - mmc1.pdf
", "abstract": "The sense organs of the vertebrate head comprise structures as varied as the eye, inner ear, and olfactory epithelium. In the early embryo, these assorted structures share a common developmental origin within the preplacodal region and acquire specific characteristics only later. Here we demonstrate a fundamental similarity in placodal precursors: in the chick all are specified as lens prior to acquiring features of specific sensory or neurogenic placodes. Lens specification becomes progressively restricted in the head ectoderm, initially by FGF and subsequently by signals derived from migrating neural crest cells. We show that FGF8 from the anterior neural ridge is both necessary and sufficient to promote olfactory fate in adjacent ectoderm. Our results reveal that placode precursors share a common ground state as lens and progressive restriction allows the full range of placodal derivatives to form.", "date": "2006-10", "date_type": "published", "publication": "Developmental Cell", "volume": "11", "number": "4", "publisher": "Cell Press", "pagerange": "505-517", "id_number": "CaltechAUTHORS:20160129-134003386", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160129-134003386", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Fight for Sight" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "D010659/1" } ] }, "doi": "10.1016/j.devcel.2006.08.009", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/k0sna-h0320/files/mmc1.pdf" }, "resource_type": "article", "pub_year": "2006", "author_list": "Bailey, Andrew P.; Bhattacharyya, Sujata; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jr4mn-h2630", "eprint_id": 59501, "eprint_status": "archive", "datestamp": "2023-08-19 18:41:30", "lastmod": "2023-10-23 20:40:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bailey-A-P", "name": { "family": "Bailey", "given": "Andrew P." } }, { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Streit-A", "name": { "family": "Streit", "given": "Andrea" }, "orcid": "0000-0001-7664-7917" } ] }, "title": "Lens specification is the ground state of all sensory placodes, from which FGF promotes olfactory identity", "ispublished": "pub", "full_text_status": "public", "keywords": "DEVBIO", "note": "\u00a9 2006 Elsevier. \n\nWe thank Sharon Pudaruth for excellent technical assistance and Claudio Stern for critical comments on the manuscript. This work was funded by grant USPHS DE16459 to M.B.-F., and by a Fight for Sight studentship and a BBSRC project grant (D010659/1) to A.S.\n\nSupplemental Material - mmc1.pdf
", "abstract": "The sense organs of the vertebrate head comprise structures as varied as the eye, inner ear, and olfactory epithelium. In the early embryo, these assorted structures share a common developmental origin within the preplacodal region and acquire specific characteristics only later. Here we demonstrate a fundamental similarity in placodal precursors: in the chick all are specified as lens prior to acquiring features of specific sensory or neurogenic placodes. Lens specification becomes progressively restricted in the head ectoderm, initially by FGF and subsequently by signals derived from migrating neural crest cells. We show that FGF8 from the anterior neural ridge is both necessary and sufficient to promote olfactory fate in adjacent ectoderm. Our results reveal that placode precursors share a common ground state as lens and progressive restriction allows the full range of placodal derivatives to form.", "date": "2006-10", "date_type": "published", "publication": "Developmental Cell", "volume": "11", "number": "4", "publisher": "Cell Press", "pagerange": "505-17", "id_number": "CaltechAUTHORS:20150813-133335246", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150813-133335246", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE16459" }, { "agency": "Fight for Sight" }, { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "D010659/1" } ] }, "doi": "10.1016/j.devcel.2006.08.009", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/jr4mn-h2630/files/mmc1.pdf" }, "resource_type": "article", "pub_year": "2006", "author_list": "Bailey, Andrew P.; Bhattacharyya, Sujata; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6366x-nn634", "eprint_id": 63436, "eprint_status": "archive", "datestamp": "2023-08-19 18:22:33", "lastmod": "2023-10-25 23:49:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sauka-Spengler-T", "name": { "family": "Sauka-Spengler", "given": "Tatjana" }, "orcid": "0000-0001-9289-0263" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Development and evolution of the migratory neural crest: a gene regulatory perspective", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2006 Elsevier Ltd. \n\nAvailable online 21st June 2006. \n\nWe are grateful to Drs Sujata Bhattacharyya, Laura Gammill, Daniel Meulemans and Vivian Lee for advice and critical comments on the manuscript.", "abstract": "The neural crest, a uniquely vertebrate characteristic, gives rise to pigment cells, much of the peripheral nervous system, the craniofacial skeleton, and a plethora of other cell types. Classical embryological studies have revealed important details about the migratory pathways followed by these cells, and their subsequent differentiation into diverse derivatives. More recently, many aspects of the molecular cascade of events involved in neural crest induction and generation of these migratory cells have been revealed. Formation of the neural crest appears to involve a network of interactions whereby signaling molecules initiate the induction and, subsequently, the establishment of the neural plate border, which is marked by expression of a characteristic set of transcription factors designated as neural plate border-specifiers. These in turn regulate other transcription factors termed neural crest-specifiers, which control genes involved in neural crest delamination, the generation of migratory cells and ultimately the acquisition of appropriate fates.", "date": "2006-08", "date_type": "published", "publication": "Current Opinion in Genetics and Development", "volume": "16", "number": "4", "publisher": "Elsevier", "pagerange": "360-366", "id_number": "CaltechAUTHORS:20160106-144136264", "issn": "1879-0380", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160106-144136264", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.gde.2006.06.006", "resource_type": "article", "pub_year": "2006", "author_list": "Sauka-Spengler, Tatjana and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8ety7-jzp93", "eprint_id": 56071, "eprint_status": "archive", "datestamp": "2023-08-19 18:00:52", "lastmod": "2023-10-20 23:37:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Importance of SoxE in neural crest development and the evolution of the pharynx", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2006 Nature Publishing Group.\n\nReceived 14 December 2005; Accepted 3 March 2006.\n\nWe thank J. Langeland for providing embryonic cDNA libraries; D. Meulemans for SoxE oligonucleotide primer sequences, T. Sauka-Spengler for SoxE2 and SoxE3 library screens, R. Bergstedt and the staff at Hammond Bay Biological Station for facilities and technical assistance; R. Kusakabe for providing the LjMA2 DNA construct, and L. Trinh for assistance with confocal microscopy imaging techniques. This work was supported by a grant to M.B.F. from the National Aeronautics and Space Administration.\n\nSupplemental Material - nature04691-s1.pdf
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Supplemental Material - nature04691-s3.mov
Supplemental Material - nature04691-s4.mov
", "abstract": "The neural crest, a defining character of vertebrates, is of prime importance to their evolutionary origin. To understand neural crest evolution, we explored molecular mechanisms underlying craniofacial development in the basal jawless vertebrate, sea lamprey (Petromyzon marinus), focusing on the SoxE (Sox8, Sox9 and Sox10) gene family. In jawed vertebrates, these are important transcriptional regulators of the neural crest, and the loss of Sox9 causes abnormal craniofacial development. Here we report that two lamprey SoxE genes are expressed in migrating neural crest and crest-derived prechondrocytes in posterior branchial arches, whereas a third paralogue is expressed later in the perichondrium and mandibular arch. Morpholino knock-down of SoxE1 reveals that it is essential for posterior branchial arch development, although the mandibular arch is unaffected. The results show that chondrogenic function of SoxE regulators can be traced to the lamprey\u2013gnathostome common ancestor and indicate that lamprey SoxE genes might have undergone independent duplication to have distinct functions in mandibular versus caudal branchial arches. This work sheds light on the homology of vertebrate branchial arches and supports their common origin at the base of vertebrates.", "date": "2006-06-08", "date_type": "published", "publication": "Nature", "volume": "441", "number": "7094", "publisher": "Nature Publishing Group", "pagerange": "750-752", "id_number": "CaltechAUTHORS:20150325-104909857", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150325-104909857", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NASA" } ] }, "doi": "10.1038/nature04691", "primary_object": { "basename": "nature04691-s1.pdf", "url": "https://authors.library.caltech.edu/records/8ety7-jzp93/files/nature04691-s1.pdf" }, "related_objects": [ { "basename": "nature04691-s2.mov", "url": "https://authors.library.caltech.edu/records/8ety7-jzp93/files/nature04691-s2.mov" }, { "basename": "nature04691-s3.mov", "url": "https://authors.library.caltech.edu/records/8ety7-jzp93/files/nature04691-s3.mov" }, { "basename": "nature04691-s4.mov", "url": "https://authors.library.caltech.edu/records/8ety7-jzp93/files/nature04691-s4.mov" } ], "resource_type": "article", "pub_year": "2006", "author_list": "McCauley, David W. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ppe5c-nzk31", "eprint_id": 56258, "eprint_status": "archive", "datestamp": "2023-08-19 17:51:08", "lastmod": "2023-10-23 15:14:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Basch-M-L", "name": { "family": "Basch", "given": "Mart\u00edn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Martin I." } } ] }, "title": "Specification of the neural crest occurs during gastrulation and requires Pax7", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2006 Nature Publishing Group.\n\nReceived 10 November 2005; Accepted 23 February 2006.\n\nWe thank A. Streit, S. Fraser, A. Groves, L. Gammill, V. Lee, J. Sechrist and T. Moreno for comments and discussions; S. Mackem, D. Wilkinson, E. Matsunaga, A. Kawakami, O. Pourquie\u00b4 and H. Kondoh for gifts of Tbx6l, cMsx1, full-length cPax3, full-length cPax7, BMP4, Sox2, Dlx5 and Gata2 probes and constructs; and D. Arce and Y. Liu for technical assistance. This work was supported by a USPHS grant to M.B.-F.\n\nSupplemental Material - nature04684-s1.doc
Supplemental Material - nature04684-s2.doc
", "abstract": "The neural crest is a stem population critical for development of the vertebrate craniofacial skeleton and peripheral ganglia. Neural crest cells originate along the border between the neural plate and epidermis, migrate extensively and generate numerous derivatives, including neurons and glia of the peripheral nervous system, melanocytes, bone and cartilage of the head skeleton. Impaired neural crest development is associated with human defects, including cleft palate. Classically, the neural crest has been thought to form by interactions at the border between neural and non-neural ectoderm or mesoderm, and defined factors such as bone morphogenetic proteins (BMPs) and Wnt proteins have been postulated as neural crest-inducers. Although competence to induce crest cells declines after stage 10 (ref. 14), little is known about when neural crest induction begins in vivo. Here we report that neural crest induction is underway during gastrulation and well before proper neural plate appearance. We show that a restricted region of chick epiblast (stage 3\u20134) is specified to generate neural crest cells when explanted under non-inducing conditions. This region expresses the transcription factor Pax7 by stage 4 + and later contributes to neural folds and migrating neural crest. In chicken embryos, Pax7 is required for neural crest formation in vivo, because blocking its translation inhibits expression of the neural crest markers Slug, Sox9, Sox10 and HNK-1. Our results indicate that neural crest specification initiates earlier than previously assumed, independently of mesodermal and neural tissues, and that Pax7 has a crucial function during neural crest development.", "date": "2006-05-11", "date_type": "published", "publication": "Nature", "volume": "441", "number": "7090", "publisher": "Nature Publishing Group", "pagerange": "218-222", "id_number": "CaltechAUTHORS:20150331-151012740", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150331-151012740", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)" } ] }, "doi": "10.1038/nature04684", "primary_object": { "basename": "nature04684-s1.doc", "url": "https://authors.library.caltech.edu/records/ppe5c-nzk31/files/nature04684-s1.doc" }, "related_objects": [ { "basename": "nature04684-s2.doc", "url": "https://authors.library.caltech.edu/records/ppe5c-nzk31/files/nature04684-s2.doc" } ], "resource_type": "article", "pub_year": "2006", "author_list": "Basch, Mart\u00edn L.; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2tpsj-za119", "eprint_id": 42320, "eprint_status": "archive", "datestamp": "2023-08-19 17:13:14", "lastmod": "2023-10-25 15:49:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Taneyhill-L-A", "name": { "family": "Taneyhill", "given": "Lisa A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Recycling signals in the neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2006 BioMed Central Ltd.\n\nPublished: 9 January 2006.\n\nL.A.T. is supported by NIH NRSA fellowship 1F32 HD043535-01A2.\nM.B.-F. is supported by NIH grants NS36585 and NS051051.\n\nPublished - jbiol31.pdf
", "abstract": "Vertebrate neural crest cells are multipotent and differentiate into structures that include\ncartilage and the bones of the face, as well as much of the peripheral nervous system.\nUnderstanding how different model vertebrates utilize signaling pathways reiteratively during\nvarious stages of neural crest formation and differentiation lends insight into human disorders\nassociated with the neural crest.", "date": "2006-01-09", "date_type": "published", "publication": "Journal of Biology", "volume": "4", "publisher": "BioMed Central", "pagerange": "Art. No. 10", "id_number": "CaltechAUTHORS:20131107-150940863", "issn": "1475-4924", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20131107-150940863", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH Predoctoral Fellowship", "grant_number": "1F32 HD043535-01A2" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS051051" } ] }, "doi": "10.1186/jbiol31", "pmcid": "PMC1414065", "primary_object": { "basename": "jbiol31.pdf", "url": "https://authors.library.caltech.edu/records/2tpsj-za119/files/jbiol31.pdf" }, "resource_type": "article", "pub_year": "2006", "author_list": "Taneyhill, Lisa A. and Bronner-Fraser, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/gc9bm-wfg73", "eprint_id": 63846, "eprint_status": "archive", "datestamp": "2023-08-22 04:56:56", "lastmod": "2023-10-17 17:13:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coles-E-G", "name": { "family": "Coles", "given": "Edward G." } }, { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Miner-J-H", "name": { "family": "Miner", "given": "Jeffrey H." }, "orcid": "0000-0002-1510-8714" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Abnormalities in neural crest cell migration in laminin \u03b15 mutant mice", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Extracellular matrix; Neural crest; Chick; Mouse; Laminin; Ganglia; Cell migration", "note": "\u00a9 2005 Elsevier Inc. \n\nReceived for publication 14 June 2005, revised 11 October 2005, accepted 16 October 2005. Available online 28 November 2005. \n\nThis work was supported by grants from the American Heart Association (0525037Y to E.G.C.) and NIH (DE15309 to L.S.G. and NS051051 to M.B.F.). We would like to thank David Anderson for the mouse NeuroD probe, Kirsten Kuhlbrodt and Michael Wegner for the mouse Sox10, Lou Reichardt for the anti-p75 antibody, Peter Lwigale for the critical reading of the manuscript and Joaquin Gutierrez for the excellent animal husbandry.", "abstract": "Although numerous in vitro experiments suggest that extracellular matrix molecules like laminin can influence neural crest migration, little is known about their function in the embryo. Here, we show that laminin \u03b15, a gene up-regulated during neural crest induction, is localized in regions of newly formed cranial and trunk neural folds and adjacent neural crest migratory pathways in a manner largely conserved between chick and mouse. In laminin \u03b15 mutant mice, neural crest migratory streams appear expanded in width compared to wild type. Conversely, neural folds exposed to laminin \u03b15 in vitro show a reduction by half in the number of migratory neural crest cells. During gangliogenesis, laminin \u03b15 mutants exhibit defects in condensing cranial sensory and trunk sympathetic ganglia. However, ganglia apparently recover at later stages. These data suggest that the laminin \u03b15 subunit functions as a cue that restricts neural crest cells, focusing their migratory pathways and condensation into ganglia. Thus, it is required for proper migration and timely differentiation of some neural crest populations.", "date": "2006-01-01", "date_type": "published", "publication": "Developmental Biology", "volume": "289", "number": "1", "publisher": "Elsevier", "pagerange": "218-228", "id_number": "CaltechAUTHORS:20160121-135400492", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-135400492", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "0525037Y" }, { "agency": "NIH", "grant_number": "DE15309" }, { "agency": "NIH", "grant_number": "NS051051" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2005.10.031", "resource_type": "article", "pub_year": "2006", "author_list": "Coles, Edward G.; Gammill, Laura S.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1mmty-3xs55", "eprint_id": 23890, "eprint_status": "archive", "datestamp": "2023-08-22 04:52:49", "lastmod": "2023-10-23 20:07:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Gonzales-C", "name": { "family": "Gonzalez", "given": "Constanza" } }, { "id": "Gu-Chenghua", "name": { "family": "Gu", "given": "Chenghua" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling", "ispublished": "pub", "full_text_status": "public", "keywords": "trunk neural crest migration; sclerotome; neuropilin 2; semaphorin 3F; mouse; chick", "note": "\u00a9 2006 Company of Biologists.\nAccepted October 27, 2005. Published online before print November 30, 2005. We are indebted to David Ginty for providing the Npn2 knockout mice and Sema3f mutant embryos, as well as helpful comments throughout the course of this work. Special thanks to Vivian Lee and York Marahrens for comments on the manuscript, and to Joaquin Gutierrez for exceptional animal care. We are grateful to Drs David Anderson, Peter Gruss, Ahmed Mansouri, Andreas Kispert, Patricia Labosky, Andreas P\u00fcschel, Kirsten Kuhlbrodt and Michael Wegner for kind gifts of plasmids, and to Lou Reichardt for contributing the\np75 antibody. Many thanks to Vivian Lee for tips on immunostaining, Christian Hochstim for advice on Neural Crest Complete Medium, Pat White and Isabelle Miletich for help with neural tube cultures, Andy Ewald for the\nAPTES protocol, and Chathurani Jayasena for the substratum choice assay protocol. This work was supported by USPHS grants DE15309 and NS051051.\n\nPublished - GAMdev06.pdf
", "abstract": "In vertebrate embryos, neural crest cells migrate only through the anterior half of each somite while avoiding the posterior half. We demonstrate that neural crest cells express the receptor neuropilin 2 (Npn2), while its repulsive ligand semaphorin 3F (Sema3f) is restricted to the posterior-half somite. In Npn2 and Sema3f mutant mice, neural crest cells lose their segmental migration pattern and instead migrate as a uniform sheet, although somite polarity itself remains unchanged. Furthermore, Npn2 is cell autonomously required for neural crest cells to avoid Sema3f in vitro. These data show that Npn2/Sema3f signaling guides neural crest migration through the somite. Interestingly, neural crest cells still condense into segmentally arranged dorsal root ganglia in Npn2 nulls, suggesting that segmental neural crest migration and segmentation of the peripheral nervous system are separable processes.", "date": "2006-01", "date_type": "published", "publication": "Development", "volume": "133", "number": "1", "publisher": "Company of Biologists", "pagerange": "99-106", "id_number": "CaltechAUTHORS:20110603-090305606", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110603-090305606", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "DE15309" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS051051" } ] }, "doi": "10.1242/dev.02187", "primary_object": { "basename": "GAMdev06.pdf", "url": "https://authors.library.caltech.edu/records/1mmty-3xs55/files/GAMdev06.pdf" }, "resource_type": "article", "pub_year": "2006", "author_list": "Gammill, Laura S.; Gonzalez, Constanza; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fgj59-wt629", "eprint_id": 65000, "eprint_status": "archive", "datestamp": "2023-08-19 17:06:13", "lastmod": "2024-01-13 16:43:11", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Basch-M-L", "name": { "family": "Basch", "given": "Mart\u00edn L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural Crest Inducing Signals", "ispublished": "unpub", "full_text_status": "restricted", "note": "\u00a9 2006 Landes Bioscience and Springer Science. \n\nWe would like to thank Dr. Andrew K. Groves for helpful comments on this manuscript.", "abstract": "The formation of the neural crest has been traditionally considered a classic example of secondary induction, where signals form one tissue elicit a response in a competent responding tissue. Interactions of the neural plate with paraxial mesoderm or nonneural ectoderm can generate neural crest. Several signaling pathways converge at the border between neural and nonneural ectoderm where the neural crest will form. Among the molecules identified in this process are members of the BMP, Wnt, FGF and Notch signaling pathways. The concerted action of these nals and their downstream targets will define the identity of the neural crest.", "date": "2006", "date_type": "published", "publisher": "Springer", "place_of_pub": "New York, NY", "pagerange": "24-31", "id_number": "CaltechAUTHORS:20160303-102135757", "isbn": "978-0-387-35136-0", "book_title": "Neural Crest Induction and Differentiation", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160303-102135757", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Saint-Jeannet-J-P", "name": { "family": "Saint-Jeannet", "given": "Jean-Pierre" } } ] }, "doi": "10.1007/978-0-387-46954-6_2", "resource_type": "book_section", "pub_year": "2006", "author_list": "Basch, Mart\u00edn L. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5n9r2-95m87", "eprint_id": 63837, "eprint_status": "archive", "datestamp": "2023-08-22 04:41:08", "lastmod": "2023-10-17 17:12:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Moreno-T-A", "name": { "family": "Moreno", "given": "Tanya A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Noelins modulate the timing of neuronal differentiation during development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Noelin-4; Noelin-1; Neurogenesis; Xenopus; Neural competence; Neural differentiation", "note": "\u00a9 2005 Elsevier Inc. \n\nReceived for publication 3 August 2005, revised 9 September 2005, accepted 21 September 2005. Available online 14 November 2005. \n\nWe are grateful to Drs. Clare Baker, Laura Gammill, Anne Knecht and Carole LaBonne for critical reading of the manuscript and helpful discussions during the course of this work. We thank Johanna Tan who provided excellent technical assistance. cDNA libraries and constructs used were kind gifts of R. Harland, M. King, and Y. Sasai. T.A.M. was a fellow of the ARCS foundation. This work was supported by NIH grant NS42287 to M.B.-F.", "abstract": "Noelins comprise a family of extracellular proteins with proposed roles in neural and neural crest development. Here, we show that a previously uncharacterized family member, Noelin-4, functions to maintain neural precursors in an undifferentiated state and biases ectoderm toward a neural fate. We show that Noelin-4 is induced by the neurogenic genes X-ngnr-1 and XNeuroD. Over-expression of Noelin-4 causes expansion of the neural plate at the expense of neural crest and epidermis. Although there is an apparent increase in the neural precursor pool, no increase was noted in differentiated neurons. Later, derivatives such as the neural tube and retina appear enlarged. We show biochemically that Noelin-4 protein is glycosylated and secreted and that it interacts with Noelin-1, an isoform previously found to promote differentiation in neuralized animal caps. Accordingly, the neural precursor expansion activity of Noelin-4 is reversed by co-expression of Noelin-1. Our finding that Noelin isoforms can bind to and antagonize one another suggests that interacting Noelin isoforms may play a role in regulating timing of differentiation.", "date": "2005-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "288", "number": "2", "publisher": "Elsevier", "pagerange": "434-447", "id_number": "CaltechAUTHORS:20160121-103519913", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-103519913", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS42287" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2005.09.050", "resource_type": "article", "pub_year": "2005", "author_list": "Moreno, Tanya A. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jwhv2-tmn82", "eprint_id": 63847, "eprint_status": "archive", "datestamp": "2023-08-22 04:35:01", "lastmod": "2023-10-17 17:13:36", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } }, { "id": "Cressy-P-A", "name": { "family": "Cressy", "given": "Paola A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Corneal keratocytes retain neural crest progenitor cell properties", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Cornea; Neural crest; Keratocyte; Differentiation", "note": "\u00a9 2005 Elsevier Inc. \n\nReceived for publication 19 August 2005, revised 27 September 2005, accepted 30 September 2005. Available online 2 November 2005. \n\nWe are grateful to Dr. James Funderburgh for providing the I22 antibody and to Anitha Rao for technical assistance. This work was supported by an Elizabeth Ross Fellowship (to PYL), and NIH grant, NS 36585 (to MBF).", "abstract": "Corneal keratocytes have a remarkable ability to heal the cornea throughout life. Given their developmental origin from the cranial neural crest, we asked whether this regenerative ability was related to the stem cell-like properties of their neural crest precursors. To this end, we challenged corneal stromal keratocytes by injecting them into a new environment along cranial neural crest migratory pathways. The results show that injected stromal keratocytes change their phenotype, proliferate and migrate ventrally adjacent to host neural crest cells. They then contribute to the corneal endothelial and stromal layers, the musculature of the eye, mandibular process, blood vessels and cardiac cushion tissue of the host. However, they fail to form neurons in cranial ganglia or branchial arch cartilage, illustrating that they are at least partially restricted progenitors rather than stem cells. The data show that, even at late embryonic stages, corneal keratocytes are not terminally differentiated, but maintain plasticity and multipotentiality, contributing to non-neuronal cranial neural crest derivatives.", "date": "2005-12-01", "date_type": "published", "publication": "Developmental Biology", "volume": "288", "number": "1", "publisher": "Elsevier", "pagerange": "284-293", "id_number": "CaltechAUTHORS:20160121-135400792", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-135400792", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Elizabeth Ross Fellowship" }, { "agency": "NIH", "grant_number": "NS 36585" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2005.09.046", "resource_type": "article", "pub_year": "2005", "author_list": "Lwigale, Peter Y.; Cressy, Paola A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/60tkf-gkn30", "eprint_id": 64081, "eprint_status": "archive", "datestamp": "2023-08-22 04:32:51", "lastmod": "2023-10-17 19:19:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Early steps in neural crest specification", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; FGF; Wnt; BMP; Slug; SoxE", "note": "\u00a9 2005 Elsevier Ltd. \n\nAvailable online 21 July 2005. \n\nWe would like to thank Paige Fraser, Yun Kee, and Ed Coles for supplying images for the figures.", "abstract": "The neural crest is a multipotent cell population that arise at the border of the neural plate and non-neural ectoderm. Studies conducted in a number of model organisms including chickens, frogs, zebrafish and mice have been instrumental in elucidating this molecular mechanisms underlying neural crest formation. Signaling molecules of the Wnt, BMP, and FGF families and their downstream effectors have been shown to mediate neural crest induction. Transcription factors including members of the Snail and SoxE gene families as well as FoxD3, c-Myc and others have been implicated in specification of the neural crest. These studies represent an important step in understanding the regulatory interactions involved in generating this complex and interesting cell type.", "date": "2005-12", "date_type": "published", "publication": "Seminars in Cell and Developmental Biology", "volume": "16", "number": "6", "publisher": "Elsevier", "pagerange": "642-646", "id_number": "CaltechAUTHORS:20160129-080804452", "issn": "1084-9521", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160129-080804452", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/j.semcdb.2005.06.006", "resource_type": "article", "pub_year": "2005", "author_list": "Barembaum, Meyer and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g9wbz-6kw51", "eprint_id": 5347, "eprint_status": "archive", "datestamp": "2023-08-22 04:21:56", "lastmod": "2023-10-16 19:10:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Taneyhill-L-A", "name": { "family": "Taneyhill", "given": "Lisa A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dynamic alterations in gene expression after Wnt-mediated induction of avian neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2005 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons\u2013Noncommercial\u2013Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). \n\nSubmitted March 15, 2005; revised August 15, 2005; accepted August 19, 2005. Originally published as MBC in Press, 10.1091/mbc.E05-03-0210 on August 31, 2005\n\nThis work was supported by NIH grant NS36585 to MBF. L.A. Taneyhill was supported in part by an NRSA fellowship from the NIH (1 F32 HD43535-01A2). Many thanks are given to Dr. Martin Garcia-Castro for technical and editorial assistance. We thank Dr. MariaElena de Bellard and Dr. Edward Coles for critical reading of the manuscript.\n\nPublished - TANmbc05.pdf
", "abstract": "The Wnt signaling pathway is important in the formation of neural crest cells in many vertebrates, but the downstream targets of neural crest induction by Wnt are largely unknown. Here, we examined quantitative changes in gene expression regulated by Wnt-mediated neural crest induction using quantitative PCR (QPCR). Induction was recapitulated in vitro by adding soluble Wnt to intermediate neural plate tissue cultured in collagen, and induced versus control tissue were assayed using gene-specific primers at times corresponding to premigratory (18 and 24 h) or early (36 h) stages of crest migration. The results show that Wnt signaling up-regulates in a distinct temporal pattern the expression of several genes normally expressed in the dorsal neural tube (slug, Pax3, Msx1, FoxD3, cadherin 6B) at \"premigratory\" stages. While slug is maintained in early migrating crest cells, Pax3, FoxD3, Msx1 and cadherin 6B all are down-regulated by the start of migration. These results differ from the temporal profile of these genes in response to the addition of recombinant BMP4, where gene expression seems to be maintained. Interestingly, expression of rhoB is unchanged or even decreased in response to Wnt-mediated induction at all times examined, though it is up-regulated by BMP signals. The temporal QPCR profiles in our culture paradigm approximate in vivo expression patterns of these genes before neural crest migration, and are consistent with Wnt being an initial neural crest inducer with additional signals like BMP and other factors maintaining expression of these genes in vivo. Our results are the first to quantitatively describe changes in gene expression in response to a Wnt or BMP signal during transformation of a neural tube cell into a migratory neural crest cell.", "date": "2005-11", "date_type": "published", "publication": "Molecular Biology of the Cell", "volume": "16", "number": "11", "publisher": "American Society for Cell Biology", "pagerange": "5283-5293", "id_number": "CaltechAUTHORS:TANmbc05", "issn": "1059-1524", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:TANmbc05", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1091/mbc.E05-03-0210", "pmcid": "PMC1266426", "primary_object": { "basename": "TANmbc05.pdf", "url": "https://authors.library.caltech.edu/records/g9wbz-6kw51/files/TANmbc05.pdf" }, "resource_type": "article", "pub_year": "2005", "author_list": "Taneyhill, Lisa A. and Bronner, Marianne E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dmhee-ct533", "eprint_id": 63417, "eprint_status": "archive", "datestamp": "2023-08-19 16:12:43", "lastmod": "2023-10-25 23:48:06", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The Neural Crest: Migrating from the Border", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2005 Wiley-VCH.", "abstract": "[no abstract]", "date": "2005-08-09", "date_type": "published", "publisher": "Wiley-VCH", "place_of_pub": "Weinheim, Germany", "pagerange": "155-171", "id_number": "CaltechAUTHORS:20160106-110411120", "isbn": "9783527305872", "book_title": "Cell Migration in Development and Disease", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160106-110411120", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Wedlich-D", "name": { "family": "Wedlich", "given": "Doris" } } ] }, "doi": "10.1002/3527604669.ch9", "resource_type": "book_section", "pub_year": "2005", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4s181-s9y39", "eprint_id": 63304, "eprint_status": "archive", "datestamp": "2023-08-19 16:09:49", "lastmod": "2023-10-25 23:42:58", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Investigator profile. An interview with Marianne Bronner-Fraser, Ph.D. Interview by Vicki Glaser", "ispublished": "pub", "full_text_status": "public", "note": "\u00a92005 Mary Ann Liebert, Inc.", "abstract": "Marianne Bronner-Fraser received her Sc.B. in Biophysics from Brown University and her Ph.D. in Biophysics from Johns Hopkins University. She joined the faculty at University of California, Irvine, in 1980 and became a Full Professor in 1990, as well as co-director of the Developmental Biology Center. In 1996, she moved to the Division of Biology at Caltech where she is currently the Albert Billings Ruddock Professor of Biology. From 2001 to 2003, she was Chair of the Faculty at Caltech. Dr. Bronner-Fraser's research centers on the early formation of the nervous system in vertebrate embryos. Her laboratory focuses on how neural crest cells and placodes arose, both in a developmental and evolutionary context. The aim is to unravel the molecular and cellular signals by which neural crest and placode cells form and evolve using a combination of embryologic, molecular, and genomic approaches.", "date": "2005-08", "date_type": "published", "publication": "Zebrafish", "volume": "2", "number": "2", "publisher": "Mary Ann Liebert", "pagerange": "71-75", "id_number": "CaltechAUTHORS:20160102-000425084", "issn": "1557-8542", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160102-000425084", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1089/zeb.2005.2.71", "resource_type": "article", "pub_year": "2005", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t6xfy-wth41", "eprint_id": 64469, "eprint_status": "archive", "datestamp": "2023-08-19 16:05:54", "lastmod": "2023-10-17 21:25:41", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Central role of gene cooption in neural crest evolution", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2005 Wiley-Liss, Inc. \n\nIssue published online: 19 Jul 2005; article first published online: 3 May 2005; manuscript accepted: 17 Feb 2005; manuscript received: 15 Dec 2004. \n\nGrant sponsors: This work was supported by USPHS grant DE13223 and NASA NAG 2-1585 to M.B.-F. The authors thank Bill Jeffery, for helpful correspondence, and the anonymous reviewers whose comments improved the quality of this work.", "abstract": "A bona fide neural crest is a defining feature of vertebrate embryos. Protochordate gene expression patterns indicate that neural crest evolution coincided with the cooption of several transcriptional regulators to the neural plate border of the vertebrate ancestor. Recent cell labeling experiments in ascidians suggest that cells in this domain may have been migratory and thus displayed some neural crest cell-like behavior. Taken together, these data suggest that the recruitment of new genetic pathways conferred novel developmental potentials upon the migratory neural tube cells of the prevertebrate chordate.", "date": "2005-07-15", "date_type": "published", "publication": "Journal of Experimental Zoology Part B: Molecular and Developmental Evolution", "volume": "304B", "number": "4", "publisher": "Wiley", "pagerange": "298-303", "id_number": "CaltechAUTHORS:20160212-102836915", "issn": "1552-5007", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-102836915", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NASA", "grant_number": "NAG 2-1585" } ] }, "doi": "10.1002/jez.b.21047", "resource_type": "article", "pub_year": "2005", "author_list": "Meulemans, Daniel and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5b2m8-env34", "eprint_id": 64483, "eprint_status": "archive", "datestamp": "2023-08-22 03:43:45", "lastmod": "2023-10-17 21:26:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McKeown-S-J", "name": { "family": "McKeown", "given": "Sonja J." } }, { "id": "Lee-V-M", "name": { "family": "Lee", "given": "Vivian M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Newgreen-D-F", "name": { "family": "Newgreen", "given": "Donald F." } }, { "id": "Farlie-P-G", "name": { "family": "Farlie", "given": "Peter G." } } ] }, "title": "Sox10 Overexpression Induces Neural Crest-Like Cells From All Dorsoventral Levels of the Neural Tube but Inhibits Differentiation", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Sox10; neural crest; HNK-1; in ovo electroporation; chick", "note": "\u00a9 2005 Wiley-Liss, Inc. \n\nReceived 16 August 2004; Revised 17 November 2004; Accepted 6 December 2004. Article first published online: 14 Mar 2005. \n\nWe thank Yoshio Wakamatsu for the MEBL-1 antibody; Yi-Chuan Cheng for chick Sox8, Sox9, and Sox10 cDNA; Mirella Dottori for FoxD3 constructs; Craig Smith for SoxE antibody, Sox8 and Sox9 in situ probes; Masatoshi Takeichi for cadherin-7 antibody; Paul Sharpe for chick Sox9 cDNA; Angela Nieto for the Slug probe; and Cathy Krull for the pMES construct. We also thank the Developmental Studies Hybridoma Bank, developed under the auspices of the NIHCD and maintained by the University of Iowa, Department of Biological Sciences (Iowa City, IA), for the following antibodies: MF20, developed by D.A. Fischmann; 1E8 (P_o), developed by E. Frank; 4C7 (HNF3\u03b2), developed by T. Jessell and S. Brenner-Morton; and 56-4H7 (RhoB) developed by T. Jessell. S.J.M. is supported by an Australian Postgraduate Award. V.M.L. is supported by an American Heart Association Postdoctoral Fellowship.", "abstract": "SoxE genes (Sox8, Sox9, and Sox10) are early response genes to neural crest induction. Although the early role of Sox9 has been examined in chick and frog, later roles in neural crest migration and differentiation remain largely unexplored. We first examined which SoxE genes were expressed in trunk neural crest cells and then investigated their function using in ovo electroporation. The results of this analysis reveal that Sox10 is present in migrating neural crest cells, whereas other SoxE genes are only expressed transiently after induction. Ectopic expression of Sox10 in the neural tube at trunk level induced expression of HNK-1 in neuroepithelial cells followed by extensive emigration from all levels of the dorsoventral neuraxis, including the floor plate. Sox10-expressing cells failed to express neuronal, Schwann, or melanocyte markers up to 6 days posttransfection (E8), suggesting these cells were maintained in an undifferentiated state. Overexpression of Sox8 or Sox9 had similar but not identical effects on neuroepithelial cells. These results show that high levels of Sox10, Sox9, or Sox8 expression in the neural tube are capable of inducing a migratory neural crest-like phenotype even in the absence of dorsal signals and can maintain these cells in an undifferentiated state.", "date": "2005-06", "date_type": "published", "publication": "Developmental Dynamics", "volume": "233", "number": "2", "publisher": "Wiley-Liss, Inc.", "pagerange": "430-444", "id_number": "CaltechAUTHORS:20160216-073205358", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160216-073205358", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Australian Research Council" }, { "agency": "American Heart Association" } ] }, "doi": "10.1002/dvdy.20341", "resource_type": "article", "pub_year": "2005", "author_list": "McKeown, Sonja J.; Lee, Vivian M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wv6zk-z1x50", "eprint_id": 15901, "eprint_status": "archive", "datestamp": "2023-08-22 03:36:42", "lastmod": "2023-10-19 17:20:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bok-Jinwoong", "name": { "family": "Bok", "given": "Jinwoong" } }, { "id": "Wu-Doris-K", "name": { "family": "Wu", "given": "Doris K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear", "ispublished": "pub", "full_text_status": "public", "keywords": "Inner ear; Axis; Axial specification; Induction; Hindbrain; Rhombomere; Sonic hedgehog; SHH; Chicken", "note": "\u00a9 The Company of Biologists Ltd 2005. \n\nAccepted 18 February 2005. First published online 23 March 2005. \n\nWe thank Drs Susan Sullivan and Thomas Friedman for critical reading of the manuscript. M.B.F. is supported by NIH RO1 DE016459. \n\nSupplementary material for this article is available at http://dev.biologists.org/cgi/content/full/132/9/2115/DC1\n\nPublished - BOKdev05.pdf
Supplemental Material - FigS1.jpg
", "abstract": "An early and crucial event in vertebrate inner ear development is the acquisition of axial identities that in turn dictate the positions of all subsequent inner ear components. Here, we focus on the role of the hindbrain in establishment of inner ear axes and show that axial specification occurs well after otic placode formation in chicken. Anteroposterior (AP) rotation of the hindbrain prior to specification of this axis does not affect the normal AP orientation and morphogenesis of the inner ear. By contrast, reversing the dorsoventral (DV) axis of the hindbrain results in changing the DV axial identity of the inner ear. Expression patterns of several ventrally expressed otic genes such as NeuroD, Lunatic fringe (Lfng) and Six1 are shifted dorsally, whereas the expression pattern of a normally dorsal-specific gene, Gbx2, is abolished. Removing the source of Sonic Hedgehog (SHH) by ablating the floor plate and/or notochord, or inhibiting SHH function using an antibody that blocks SHH bioactivity results in loss of ventral inner ear structures. Our results indicate that SHH, together with other signals from the hindbrain, are important for patterning the ventral axis of the inner ear. Taken together, our studies suggest that tissue(s) other than the hindbrain confer AP axial information whereas signals from the hindbrain are necessary and sufficient for the DV axial patterning of the inner ear.", "date": "2005-05-01", "date_type": "published", "publication": "Development", "volume": "132", "number": "9", "publisher": "Company of Biologists", "pagerange": "2115-2124", "id_number": "CaltechAUTHORS:20090917-092638969", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090917-092638969", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1 DE016459" } ] }, "doi": "10.1242/dev.01796", "primary_object": { "basename": "BOKdev05.pdf", "url": "https://authors.library.caltech.edu/records/wv6zk-z1x50/files/BOKdev05.pdf" }, "related_objects": [ { "basename": "FigS1.jpg", "url": "https://authors.library.caltech.edu/records/wv6zk-z1x50/files/FigS1.jpg" }, { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/wv6zk-z1x50/files/medium.png" }, { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/wv6zk-z1x50/files/small.png" } ], "resource_type": "article", "pub_year": "2005", "author_list": "Bok, Jinwoong; Wu, Doris K.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j8ckc-8q072", "eprint_id": 64125, "eprint_status": "archive", "datestamp": "2023-08-19 15:30:34", "lastmod": "2023-10-17 19:31:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors", "ispublished": "pub", "full_text_status": "public", "keywords": "Xenopus Id3 neural crest cell cycle survival", "note": "\u00a9 2005 Cold Spring Harbor Laboratory Press. \n\nReceived September 1, 2004; revised version accepted January 19, 2005. \n\nWe especially thank Drs. Scott Fraser, Laura Gammill, Tatjana Sauka-Spengler, Tanya Moreno, Daniel Meulemans, Peter Lwigale, and Byung Joon Hwang for critical comments on this manuscript; Samuel Ki for technical assistance; and members of our laboratories for technical advice and help. We are grateful to the people who provided plasmids, as mentioned in Materials and Methods. This work was supported by an American Heart Association Post-doctoral Fellowship to Y.K. and by NIH grant DE13223 to M.B.-F.\n\nPublished - 744.full.pdf
", "abstract": "The neural crest is a unique population of mitotically active, multipotent progenitors that arise at the vertebrate neural plate border. Here, we show that the helix-loop-helix transcriptional regulator Id3 has a novel role in cell cycle progression and survival of neural crest progenitors in Xenopus. Id3 is localized at the neural plate border during gastrulation and neurulation, overlapping the domain of neural crest induction. Morpholino oligonucleotide-mediated depletion of Id3 results in the absence of neural crest precursors and a resultant loss of neural crest derivatives. This appears to be mediated by cell cycle inhibition followed by cell death of the neural crest progenitor pool, rather than a cell fate switch. Conversely, overexpression of Id3 increases cell proliferation and results in expansion of the neural crest domain. Our data suggest that Id3 functions by a novel mechanism, independent of cell fate determination, to mediate the decision of neural crest precursors to proliferate or die.", "date": "2005-03-15", "date_type": "published", "publication": "Genes and Development", "volume": "19", "number": "6", "publisher": "Cold Spring Harbor Laboratory Press", "pagerange": "744-755", "id_number": "CaltechAUTHORS:20160201-110340978", "issn": "0890-9369", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160201-110340978", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association" }, { "agency": "NIH", "grant_number": "DE13223" } ] }, "doi": "10.1101/gad.1257405", "pmcid": "PMC1065727", "primary_object": { "basename": "744.full.pdf", "url": "https://authors.library.caltech.edu/records/j8ckc-8q072/files/744.full.pdf" }, "resource_type": "article", "pub_year": "2005", "author_list": "Kee, Yun and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vweed-6b603", "eprint_id": 63840, "eprint_status": "archive", "datestamp": "2023-08-22 03:10:28", "lastmod": "2023-10-17 17:12:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lee-V-M", "name": { "family": "Lee", "given": "Vivian M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } } ] }, "title": "Restricted response of mesencephalic neural crest to sympathetic differentiation signals in the trunk", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Mesencephalic neural crest; Cholinergic; Catecholaminergic; Quail-chick chimera; Ciliary ganglion; Sympathetic; Parasympathetic; dHAND; Tyrosine hydroxylase", "note": "\u00a9 2004 Elsevier Inc. \n\nReceived for publication 25 September 2002, revised 24 September 2004, accepted 27 October 2004. Available online 21 November 2004. \n\nWe gratefully acknowledge the advice and support of Dr. Marie-Aime\u00e9 Teillet for initial encouragement in these experiments and for comments on an earlier version of the manuscript. Thanks also to Dr. Andy Groves for comments on an earlier version of the manuscript. Thanks to Dr. Hermann Rohrer for the VAChT clone and to Dr. Jean-Fran\u00e7ois Brunet for the Phox2a clone. Thanks to Matt Jones and Anitha Rao for help with sectioning. This work was supported by NS-041070-02 from the NIH (M.B.-F.), by American Heart Association Fellowships 1171-FI1, 0020097Y (C.V.H.B.), and 0225071Y (V.M.L.).", "abstract": "Lineage diversification in the vertebrate neural crest may occur via instructive signals acting on pluripotent cells, and/or via early specification of subpopulations towards particular lineages. Mesencephalic neural crest cells normally form cholinergic parasympathetic neurons in the ciliary ganglion, while trunk neural crest cells normally form both catecholaminergic and cholinergic neurons in sympathetic ganglia. In contrast to trunk neural crest cells, mesencephalic neural crest cells apparently fail to express the catecholaminergic transcription factor dHAND in response to BMPs in the head environment. Here, we show that migrating quail mesencephalic neural crest cells grafted into the trunk of host chick embryos colonise the sympathetic ganglia. While many express dHAND and form tyrosine hydroxylase (TH)-positive catecholaminergic neurons, the proportion that expresses either dHAND or TH is significantly smaller than that of quail trunk neural crest cells under the same conditions. Furthermore, the proportion of quail mesencephalic neural crest cells that is TH^+ in the sympathetic ganglia decreases with time, while the proportion of TH^+ quail trunk neural crest-derived cells increases. Thus, a subset of mesencephalic neural crest cells fails to express dHAND or TH in the sympathetic ganglia, while a further subset initiates but fails to maintain TH expression. Taken together, our results suggest that a subpopulation of migrating mesencephalic neural crest cells is refractory to catecholaminergic differentiation signals in the trunk. We suggest that this heterogeneity, together with local signals that repress catecholaminergic differentiation, may ensure that most ciliary neurons adopt a cholinergic fate.", "date": "2005-02-01", "date_type": "published", "publication": "Developmental Biology", "volume": "278", "number": "1", "publisher": "Elsevier", "pagerange": "175-192", "id_number": "CaltechAUTHORS:20160121-103520714", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-103520714", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS-041070-02" }, { "agency": "American Heart Association", "grant_number": "1171-FI1" }, { "agency": "American Heart Association", "grant_number": "0020097Y" }, { "agency": "American Heart Association", "grant_number": "0225071Y" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2004.10.024", "resource_type": "article", "pub_year": "2005", "author_list": "Lee, Vivian M.; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/brj26-nqf04", "eprint_id": 63302, "eprint_status": "archive", "datestamp": "2023-08-19 15:08:09", "lastmod": "2023-10-25 23:42:53", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Hierarchy of events regulating neural crest induction", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2005 John Wiley & Sons.", "abstract": "The neural crest is a group of cells that has a transient existence in the developing vertebrate embryo. Arising at the edges of the neural plate during nervous a formation, these cells initiate life within the central nervous system but soon leave to invade the far reaches of the body (Fig. 7.1). They undergo an epithelial to mesenchymal transition, emigrate from the neuroepithelium, and become a highly migratory cell type that follows distinct and characteristic pathways to numerous sites. They populate most of the peripheral nervous system, parts of the adrenal gland, and much of the facial region. Upon reaching their final destinations, these cells differentiate into a myriad of cell types ranging from pigment cells to sensory and autonomic neurons to cartilage and bone of the face.", "date": "2005", "date_type": "published", "publisher": "Wiley-Liss", "place_of_pub": "New York, NY", "pagerange": "129-44", "id_number": "CaltechAUTHORS:20160101-233617295", "isbn": "0-471-73212-5", "book_title": "The Harvey Lectures", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-233617295", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "book_section", "pub_year": "2005", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7503j-1qj74", "eprint_id": 63303, "eprint_status": "archive", "datestamp": "2023-08-22 02:54:59", "lastmod": "2024-01-13 16:30:38", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Elena-de-Bellard-M", "name": { "family": "Elena de Bellard", "given": "Maria" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest migration methods in the chicken embryo", "ispublished": "unpub", "full_text_status": "public", "keywords": "Neural crest cells; electroporation; live cell labeling; confocal imaging; whole mount; immunohistochemistry; immunolabeling; migration; chicken embryo", "note": "\u00a9 2005 Humana Press.", "abstract": "Neural crest cells emerge from the neural tube early in development. They migrate extensively throughout the embryo and form most of the head and peripheral nervous system, giving rise to sensory and sympathetic ganglia, heart regions, adrenal cells, head bones, teeth, muscle cells, sensory organs, melanocytes, and other cell types. The neural crest is interesting because of its unique origin, development and differentiation. These cells are initially part of the dorsal neural tube, with a clear epithelial character; later, they transform into actively motile mesenchymal cells. Little is known about the underlying mechanism directing this process. It remains unknown why neural crest cells target particular derivatives (neurons, heart muscle and glia) and body regions (peripheral nerves, heart, skin, head and gut). Neural crest migration can be divided into three stages: 1) emigration from the neural tube; 2) migration along defined pathways; and 3) cessation of migration. At the onset of migration, neural crest cells lose their epithelial nature within the neural tube and transform into a migratory, mesenchymal cell type. Neural crest development has been best studied in avian embryos, which are amenable to surgical manipulation, cell marking techniques, cell culture and transgenesis by electroporation and retrovirally mediate gene transfer. The methods outlined below are those typically used to study and understand the different factors and signals necessary for the neural crest development before and during their migration.", "date": "2005", "date_type": "published", "publisher": "Humana Press", "place_of_pub": "Clinton, NJ", "pagerange": "247-67", "id_number": "CaltechAUTHORS:20160101-235847845", "isbn": "978-1-58829-382-4", "book_title": "Cell Migration: Developmental Methods and Protocols", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-235847845", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Guan-Jun-Lin", "name": { "family": "Guan", "given": "Jun-Lin" } } ] }, "doi": "10.1385/1-59259-860-9:247", "resource_type": "book_section", "pub_year": "2005", "author_list": "Elena de Bellard, Maria and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/f0yn6-jww75", "eprint_id": 25364, "eprint_status": "archive", "datestamp": "2023-08-19 14:38:41", "lastmod": "2023-10-24 15:49:12", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coles-E-G", "name": { "family": "Coles", "given": "Edward" } }, { "id": "Christiansen-Jeff", "name": { "family": "Christiansen", "given": "Jeff" } }, { "id": "Economou-A", "name": { "family": "Economou", "given": "Androulla" } }, { "id": "Wilkinson-D-G", "name": { "family": "Wilkinson", "given": "David G." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A vertebrate crossveinless 2 homologue modulates BMP activity\n and neural crest cell migration", "ispublished": "pub", "full_text_status": "public", "keywords": "BMP activity, Neural crest, Cell migration, Chick, Xenopus", "note": "\u00a9 2004 The Company of Biologists. \n\nPublished online before print September 29, 2004. \n\nWe thank Dalit Sela-Donenfeld and Siew-Lan Ang for comments on the manuscript, Yi-Chuan Cheng for chick Sox10 probe, Mike Jones for Xbra probe, Elizabeth Robertson for BMP4-Myc expression construct, Ian Harragan for histological sectioning, Lisa Taneyhill for advice on immunoprecipitations, and Nobue Itasaki and Branko\nLatinkic for advice on Xenopus microinjections.\n\nPublished - COLdev04.pdf
", "abstract": "Previous work has revealed that proteins that bind to bone morphogenetic proteins (BMPs) and inhibit their signalling have a crucial role in the spatial and temporal regulation of cell differentiation and cell migration by BMPs. We have identified a chick homologue of crossveinless 2, a Drosophila gene that was identified in genetic studies as a promoter of BMP-like signalling. Chick Cv-2 has a conserved structure of five cysteine-rich repeats similar to those found in several BMP antagonists, and a C-terminal Von Willebrand type D domain. Cv-2 is expressed in the chick embryo in a number of tissues at sites at which elevated BMP signalling is required. One such site of expression is premigratory neural crest, in which at trunk levels threshold levels of BMP activity are required to initiate cell migration. We show that, when overexpressed, Cv-2 can weakly antagonise BMP4 activity in Xenopus embryos, but that in other in vitro assays Cv-2 can increase the activity of co-expressed BMP4. Furthermore, we find that increased expression of Cv-2 causes premature onset of trunk neural crest cell migration in the chick embryo, indicative of Cv-2 acting to promote BMP activity at an endogenous site of expression. We therefore propose that BMP signalling is modulated both by antagonists and by Cv-2 that acts to elevate BMP activity.", "date": "2004-11-01", "date_type": "published", "publication": "Development", "volume": "131", "number": "21", "publisher": "Company of Biologists", "pagerange": "5309-5317", "id_number": "CaltechAUTHORS:20110920-103005137", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20110920-103005137", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1242/dev.01419", "primary_object": { "basename": "COLdev04.pdf", "url": "https://authors.library.caltech.edu/records/f0yn6-jww75/files/COLdev04.pdf" }, "resource_type": "article", "pub_year": "2004", "author_list": "Coles, Edward; Christiansen, Jeff; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1z122-3gw78", "eprint_id": 64873, "eprint_status": "archive", "datestamp": "2023-08-19 14:37:13", "lastmod": "2023-10-17 21:56:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Conservation and divergence of BMP2/4 genes in the lamprey: expression and phylogenetic analysis suggest a single ancestral vertebrate gene", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 Blackwell Publishing. \n\nArticle first published online: 27 Oct 2004. \n\nWe thank Daniel Meulemans, Tatjana Sauka-Spengler, and two\nanonymous reviewers for helpful comments to improve this manuscript. We also thank Roger Bergstedt and the staff at Hammond Bay Biological Station for aid in collecting and rearing embryos, and Jim Langeland for embryonic cDNA libraries. Supported by NASA grant NAG2-1585 to M. B. F.", "abstract": "Bone morphogenetic protein (BMP) molecules are members of a large family of signaling molecules important in numerous developmental pathways throughout the metazoa. Single members of the BMP2/4 class have been found in invertebrates such as cnidarians, arthropods, nematodes, echinoderms, ascidians, and cephalochordates. In all vertebrates studied, there are at least two copies, BMP2 and BMP4, that play important roles in axial patterning, tissue specification, and organogenesis. The basal vertebrate, lamprey, diverged near the time of vertebrate origins and is useful for understanding the gene duplication events that led to the increased complexity of the vertebrate genome. We characterized the sequence and expression pattern of BMP2/4 class genes in the sea lamprey, Petromyzon marinus. We uncovered three genes that we named PmBMP2/4A, PmBMP2/4B, and PmBMP2/4C. Phylogenetic analysis indicates that PmBMP2/4A is closer than PmBMP2/4B or PmBMP2/4C in sequence identity to both BMP2 and BMP4 ofgnathostomes. The developmental expression pattern of PmBMP2/4A also more closely resembles the combined early expression patterns of gnathostome BMP2 and BMP4, whereas PmBMP2/4B and PmBMP2/4C appear to play roles only later in development. Cell labeling showed that the BMP-expressing cells in the branchial arches of lampreys are of neural crest origin. Taken together, our sequence and expression data support the duplication of BMP2/4 genes in the lamprey from a single ancestral vertebrate BMP2/4 gene.", "date": "2004-11", "date_type": "published", "publication": "Evolution & Development", "volume": "6", "number": "6", "publisher": "Wiley", "pagerange": "411-422", "id_number": "CaltechAUTHORS:20160229-151057026", "issn": "1520-541X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160229-151057026", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NASA", "grant_number": "NAG2-1585" } ] }, "doi": "10.1111/j.1525-142X.2004.04054.x", "resource_type": "article", "pub_year": "2004", "author_list": "McCauley, David W. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b6jbe-v6j88", "eprint_id": 63839, "eprint_status": "archive", "datestamp": "2023-08-22 02:34:15", "lastmod": "2023-10-17 17:12:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCabe-K-L", "name": { "family": "McCabe", "given": "Kathryn L." } }, { "id": "Manzo-A", "name": { "family": "Manzo", "given": "Andrea" } }, { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Discovery of genes implicated in placode formation", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Placodes; Trigeminal; Olfactory; Otic; Epibranchial; Subtraction; Induction; Neural crest; Chicken", "note": "\u00a9 2004 Elsevier Inc. \n\nReceived for publication 11 May 2004, revised 13 July 2004, accepted 14 July 2004. Available online 21 August 2004. \n\nWe would like to thank Anitha Rao for technical support, Titus Brown for his help with bioinformatics programs, Dr. Jonathan Rast for answering questions regarding the library screen, and Drs. Vivian Lee and Lisa Taneyhill Ziemer for their critical reviews of the manuscript. This work was funded in part by NIH F32 DE14131-03 and NIH RO1 DE016459-05.", "abstract": "The peripheral nervous system of the head is derived from cranial ectodermal placodes and neural crest cells. Placodes arise from thickenings in the cranial ectoderm that invaginate or ingress to form sensory ganglia and the paired sense organs. We have combined embryological techniques with array technology to identify genes that are expressed as a consequence of placode induction. As a secondary screen, we used whole mount in situ hybridization to determine the expression of candidate genes in various placodal domains. The results reveal 52 genes that are found in one or more placodes, including the olfactory, trigeminal, and otic placodes. Expression of some of these genes is retained in placodal derivatives. Furthermore, several genes are common to both neural crest and ectodermal placodes. This study presents the first array of candidate genes implicated in placode development, providing numerous new molecular markers for various stages of placode formation. Importantly, the results uncover previously unknown commonalities in genes expressed by multiple placodes and shared properties between placodes and other migratory cells, like neural crest cells.", "date": "2004-10-15", "date_type": "published", "publication": "Developmental Biology", "volume": "274", "number": "2", "publisher": "Elsevier", "pagerange": "462-477", "id_number": "CaltechAUTHORS:20160121-103520420", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-103520420", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "F32 DE14131-03" }, { "agency": "NIH", "grant_number": "RO1 DE016459-05" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2004.07.012", "resource_type": "article", "pub_year": "2004", "author_list": "McCabe, Kathryn L.; Manzo, Andrea; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4sxg8-sax57", "eprint_id": 59502, "eprint_status": "archive", "datestamp": "2023-08-19 14:22:02", "lastmod": "2023-10-23 20:40:05", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Hierarchy of regulatory events in sensory placode development", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2004 Elsevier. \n\nThis review comes from a themed issue on Differentiation and gene regulation. Edited by Michael G Rosenfeld and Christopher K Glass. \n\nWe are very grateful to Drs. Andrea Streit, Andy Groves, Laura Gammill, Tatjana Sauka-Spengler and Subhabrata Sanyal for their advice and comments on the manuscript. We wish to apologize to those authors whose work we were unable to cite in this review because of space constraints. Supported by NIH grant DE 016459 to MBF. and a Howard Hughes predoctoral fellowship to SB.", "abstract": "Cranial placodes are a uniquely vertebrate characteristic; they form the paired sense organs of the eyes, ears and nose, in addition to the distal parts of some of the cranial sensory ganglia. These focal ectodermal thickenings have been studied from an embryological perspective in a diversity of organisms, revealing tissue interactions that are crucial for the morphological formation of the different placodes. In recent times, there has been a renewed interest in understanding the induction and differentiation of these deceptively simple ectodermal regions. This has led to a wealth of information on the molecular cues governing these processes. In particular, the integration of signals at the level of 'placode-specific' enhancers is beginning to provide a glimpse into the complexity of genetic networks that function within this embryonic cell population to generate key components of the peripheral nervous system.", "date": "2004-10", "date_type": "published", "publication": "Current Opinion in Genetics and Development", "volume": "14", "number": "5", "publisher": "Elsevier", "pagerange": "520-526", "id_number": "CaltechAUTHORS:20150813-133336002", "issn": "1879-0380", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150813-133336002", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE016459" }, { "agency": "Howard Hughes Medical Institute (HHMI)" } ] }, "doi": "10.1016/j.gde.2004.08.002", "resource_type": "article", "pub_year": "2004", "author_list": "Bhattacharyya, Sujata and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/37w8h-wem35", "eprint_id": 64099, "eprint_status": "archive", "datestamp": "2023-08-19 14:10:21", "lastmod": "2023-10-17 19:30:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Gene-Regulatory Interactions in Neural Crest Evolution and Development", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2004 Cell Press. \n\nThe authors wish to express their sincerest thanks to Robb Krumlauf, Eric Davidson, Ellen Rothenberg, and Scott Fraser for their invaluable inspiration and feedback, as well as to the anonymous reviewers whose thoughtful suggestions greatly improved the quality of this work. This work was supported by USPHS grant DE13223 and NASA NAG 2-1585 to M.B.-F.", "abstract": "In this review, we outline the gene-regulatory interactions driving neural crest development and compare these to a hypothetical network operating in the embryonic ectoderm of the cephalochordate amphioxus. While the early stages of ectodermal patterning appear conserved between amphioxus and vertebrates, later activation of neural crest-specific factors at the neural plate border appears to be a vertebrate novelty. This difference may reflect co-option of genetic pathways which conferred novel properties upon the evolving vertebrate neural plate border, potentiating the evolution of definitive neural crest.", "date": "2004-09", "date_type": "published", "publication": "Developmental Cell", "volume": "7", "number": "3", "publisher": "Cell Press", "pagerange": "291-299", "id_number": "CaltechAUTHORS:20160129-132837174", "issn": "1534-5807", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160129-132837174", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NASA", "grant_number": "NAG 2-1585" } ] }, "doi": "10.1016/j.devcel.2004.08.007", "resource_type": "article", "pub_year": "2004", "author_list": "Meulemans, Daniel and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rdsce-men11", "eprint_id": 59503, "eprint_status": "archive", "datestamp": "2023-08-22 02:10:47", "lastmod": "2023-10-23 20:40:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bhattacharyya-S", "name": { "family": "Bhattacharyya", "given": "Sujata" } }, { "id": "Bailey-A-P", "name": { "family": "Bailey", "given": "Andrew P." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Streit-A", "name": { "family": "Streit", "given": "Andrea" }, "orcid": "0000-0001-7664-7917" } ] }, "title": "Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Chick; Distal-less; Dlx; Eye; Eyeless; Fate map; Nasal epithelium; Pax6; Placode; Sensory organ", "note": "\u00a9 2004 Elsevier. \n\nReceived for publication 21 April 2004, accepted 23 April 2004, Available online 25 May 2004. \n\nWe thank Drs. A. Bang and D. Kosher for cDNA clones, Jhumku Kohtz and Joram Piatigorski for antibodies, and Heide Olsen for excellent technical assistance. We also thank Dan Darcy, Carole Lu, Ying Gong and Seth Ruffins for invaluable advice on confocal time-lapse movies and their analysis. We are grateful to Dr. C.D. Stern for critical reading of the manuscript. This work was supported by BBSRC 29/G13701 and a Royal Society grant to AS and by NS41070 to MBF. S.B. is supported by a Howard Hughes Predoctoral Fellowship and A.B. is supported by a Fight for Sight Studentship.", "abstract": "Cranial placodes are focal regions of columnar epithelium next to the neural tube that contribute to sensory ganglia and organs in the vertebrate head, including the olfactory epithelium and the crystalline lens of the eye. Using focal dye labelling within the presumptive placode domain, we show that lens and nasal precursors arise from a common territory surrounding the anterior neural plate. They then segregate over time and converge to their final positions in discrete placodes by apparently directed movements. Since these events closely parallel the separation of eye and antennal primordia (containing olfactory sensory cells) from a common imaginal disc in Drosophila, we investigated whether the vertebrate homologues of Distalless (Dll) and Eyeless (Ey), which determine antennal and eye identity in the fly, play a role in segregation of lens and nasal precursors in the chick. Dlx5 and Pax6 are initially co-expressed by future lens and olfactory cells. As soon as presumptive lens cells acquire columnar morphology all Dlx family members are down-regulated in the placode, while Pax6 is lost in the olfactory region. Lens precursor cells that express ectopic Dlx5 never acquire lens-specific gene expression and are excluded from the lens placode to cluster in the head ectoderm. These results suggest that the loss of Dlx5 is required for cells to adopt a lens fate and that the balance of Pax6 and Dlx expression regulates cell sorting into appropriate placodal domains.", "date": "2004-07-15", "date_type": "published", "publication": "Developmental Biology", "volume": "271", "number": "2", "publisher": "Elsevier", "pagerange": "403-14", "id_number": "CaltechAUTHORS:20150813-133336793", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150813-133336793", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Biotechnology and Biological Sciences Research Council (BBSRC)", "grant_number": "29/G13701" }, { "agency": "Royal Society" }, { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "Howard Hughes Medical Institute (HHMI)" }, { "agency": "Fight for Sight Studentship" } ] }, "doi": "10.1016/j.ydbio.2004.04.010", "resource_type": "article", "pub_year": "2004", "author_list": "Bhattacharyya, Sujata; Bailey, Andrew P.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8hhe2-afe94", "eprint_id": 64471, "eprint_status": "archive", "datestamp": "2023-08-19 13:40:44", "lastmod": "2023-10-17 21:25:43", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Basch-M-L", "name": { "family": "Basch", "given": "Mart\u00edn L." } }, { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Martin I." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular Mechanisms of Neural Crest Induction", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2004 Wiley-Liss, Inc. \n\nArticle first published online: 15 Jul 2004. \n\nWe thank Dr. Scott Fraser for careful and thoughtful comments on the manuscript. NIH. Grant Numbers: NS36585, NS42287.", "abstract": "The neural crest is an embryonic cell population that originates at the border between the neural plate and the prospective epidermis. Around the time of neural tube closure, neural crest cells emigrate from the neural tube, migrate along defined paths in the embryo and differentiate into a wealth of derivatives. Most of the craniofacial skeleton, the peripheral nervous system, and the pigment cells of the body originate from neural crest cells. This cell type has important clinical relevance, since many of the most common craniofacial birth defects are a consequence of abnormal neural crest development. Whereas the migration and differentiation of the neural crest have been extensively studied, we are just beginning to understand how this tissue originates. The formation of the neural crest has been described as a classic example of embryonic induction, in which specific tissue interactions and the concerted action of signaling pathways converge to induce a multipotent population of neural crest precursor cells. In this review, we summarize the current status of knowledge on neural crest induction. We place particular emphasis on the signaling molecules and tissue interactions involved, and the relationship between neural crest induction, the formation of the neural plate and neural plate border, and the genes that are upregulated as a consequence of the inductive events.", "date": "2004-06", "date_type": "published", "publication": "Birth Defects Research Part C \u2013 Embryo Today \u2013 Reviews", "volume": "72", "number": "2", "publisher": "Wiley", "pagerange": "109-123", "id_number": "CaltechAUTHORS:20160212-103804280", "issn": "1542-975X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-103804280", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS42287" } ] }, "doi": "10.1002/bdrc.20015", "resource_type": "article", "pub_year": "2004", "author_list": "Basch, Mart\u00edn L.; Garc\u00eda-Castro, Martin I.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w3tdq-kb956", "eprint_id": 12050, "eprint_status": "archive", "datestamp": "2023-08-22 01:55:00", "lastmod": "2023-10-17 16:29:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lwigale-P-Y", "name": { "family": "Lwigale", "given": "Peter Y." } }, { "id": "Conrad-G-W", "name": { "family": "Conrad", "given": "Gary W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Graded potential of neural crest to form cornea, sensory neurons and cartilage along the rostrocaudal axis", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest, Cornea, Trigeminal ganglion, Branchial arch, Quail-chick chimera", "note": "Published by The Company of Biologists 2004. \n\nAccepted 15 January 2004. \n\nWe are grateful to Dr Paul Trainor for providing us with Hoxa2 and Hoxa3 cDNA. This work was supported by the Elizabeth Ross Fellowship (to P.Y.L.), NIH-EY00952 (to G.W.C.), and DE13223 and NS36585 (to M.B.-F.).\n\nPublished - LWIdev04.pdf
", "abstract": "Neural crest cells arising from different rostrocaudal axial levels form different sets of derivatives as diverse as ganglia, cartilage and cornea. These variations may be due to intrinsic properties of the cell populations, different environmental factors encountered during migration or some combination thereof. We test the relative roles of intrinsic versus extrinsic factors by challenging the developmental potential of cardiac and trunk neural crest cells via transplantation into an ectopic midbrain environment. We then assess long-term survival and differentiation into diverse derivatives, including cornea, trigeminal ganglion and branchial arch cartilage. Despite their ability to migrate to the periocular region, neither cardiac nor trunk neural crest contribute appropriately to the cornea, with cardiac crest cells often forming ectopic masses on the corneal surface. Similarly, the potential of trunk and cardiac neural crest to form somatosensory neurons in the trigeminal ganglion was significantly reduced compared with control midbrain grafts. Cardiac neural crest exhibited a reduced capacity to form cartilage, contributing only nominally to Meckle's cartilage, whereas trunk neural crest formed no cartilage after transplantation, even when grafted directly into the first branchial arch. These results suggest that neural crest cells along the rostrocaudal axis display a graded loss in developmental potential to form somatosensory neurons and cartilage even after transplantation to a permissive environment. Hox gene expression was transiently maintained in the cardiac neural tube and neural crest at 12 hours post-transplantation to the midbrain, but was subsequently downregulated. This suggests that long-term differences in Hox gene expression cannot account for rostrocaudal differences in developmental potential of neural crest populations in this case.", "date": "2004-05-01", "date_type": "published", "publication": "Development", "volume": "131", "number": "9", "publisher": "Company of Biologists", "pagerange": "1979-1991", "id_number": "CaltechAUTHORS:LWIdev04", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:LWIdev04", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Elizabeth Ross Fellowship" }, { "agency": "National Eye Insitute", "grant_number": "EY00952" }, { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1242/dev.01106", "primary_object": { "basename": "LWIdev04.pdf", "url": "https://authors.library.caltech.edu/records/w3tdq-kb956/files/LWIdev04.pdf" }, "resource_type": "article", "pub_year": "2004", "author_list": "Lwigale, Peter Y.; Conrad, Gary W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fekjv-3x774", "eprint_id": 64460, "eprint_status": "archive", "datestamp": "2023-08-22 01:38:40", "lastmod": "2023-10-17 21:25:17", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Chen-Yizhen", "name": { "family": "Chen", "given": "Y." } }, { "id": "Gutmann-David-H", "name": { "family": "Gutmann", "given": "D. H." }, "orcid": "0000-0002-3127-5045" }, { "id": "Haipek-Carrie-A", "name": { "family": "Haipek", "given": "C. A." } }, { "id": "Martinsen-Brad-J", "name": { "family": "Martinsen", "given": "B. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" }, { "id": "Krull-C-E", "name": { "family": "Krull", "given": "C. E." } } ] }, "title": "Characterization of chicken Nf2/merlin indicates regulatory roles in cell proliferation and migration", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neurofibromatosis 2; merlin; schwannomin; ERM proteins; muscle precursors", "note": "\u00a9 2004 Wiley-Liss, Inc. \n\nReceived 5 August 2003; Revised 17 October 2003; Accepted 4 November 2003. \n\nC.E.K., D.H.G., and M.B.F. received funding by the Muscular Dystrophy Association and the National Institutes of Health. \n\nGrant sponsor: Muscular Dystrophy Association; Grant numbers: MH59894; NS35848; Grant sponsor: National Institutes of Health; Grant number: HD15527.", "abstract": "The neurofibromatosis 2 (NF2) tumor suppressor protein merlin, or schwannomin, functions as a negative growth regulator such that inactivating mutations in Nf2 predispose humans to tumors. In addition, merlin has a critical role during embryonic development. Nf2-deficient mice die early during embryogenesis, with defects in gastrulation and extraembryonic tissues. To investigate the function of Nf2/merlin during embryonic development, we first identified the homologous Nf2 gene in chicken (cNf2) and examined the distribution of chicken merlin (c-merlin) during myogenesis. cNf2 encoded a full-length mRNA of 1,770 nucleotides and a protein of 589 residues. C-merlin shared high sequence homology and common protein motifs with vertebrate and Drosophila merlins. In addition, cNF2 functions as a negative growth regulator similar to human and Drosophila merlin in vitro. In vivo, c-merlin was expressed diffusely in the forming dermomyotome but down-regulated in migratory muscle precursors in the forelimb. As muscle formed in the limb, c-merlin expression was up-regulated. As an initial examination of c-merlin function during myogenesis, c-merlin was ectopically expressed in muscle precursors and the effects on muscle development were examined. We show that ectopic merlin expression reduces the proliferation of muscle precursors as well as their ability to migrate effectively in limb mesoderm. Collectively, these results demonstrate that c-merlin is developmentally regulated in migrating and differentiating myogenic cells, where it functions as a negative regulator of both muscle growth and motility.", "date": "2004-03", "date_type": "published", "publication": "Developmental Dynamics", "volume": "229", "number": "3", "publisher": "Wiley-Liss, Inc.", "pagerange": "541-554", "id_number": "CaltechAUTHORS:20160212-093845737", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-093845737", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Association", "grant_number": "MH59894" }, { "agency": "NIH", "grant_number": "NS35848" }, { "agency": "NIH", "grant_number": "HD15527" } ] }, "doi": "10.1002/dvdy.20002", "resource_type": "article", "pub_year": "2004", "author_list": "Chen, Y.; Gutmann, D. H.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/77rjw-d1t46", "eprint_id": 64478, "eprint_status": "archive", "datestamp": "2023-08-22 01:38:49", "lastmod": "2023-10-17 21:26:05", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Venters-S-J", "name": { "family": "Venters", "given": "Sara J." } }, { "id": "Argent-R-E", "name": { "family": "Argent", "given": "Rebecca E." } }, { "id": "Deegan-F-M", "name": { "family": "Deegan", "given": "Fiona M." } }, { "id": "Perez-Baron-G", "name": { "family": "Perez-Baron", "given": "Gina" } }, { "id": "Wong-T-S", "name": { "family": "Wong", "given": "Ted S." } }, { "id": "Tidyman-W-F", "name": { "family": "Tidyman", "given": "William E." } }, { "id": "Denetclaw-W-F", "name": { "family": "Denetclaw", "given": "Wilfred F." } }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Ordahl-C-P", "name": { "family": "Ordahl", "given": "Charles P." } } ] }, "title": "Precocious Terminal Differentiation of Premigratory Limb Muscle Precursor Cells Requires Positive Signalling", "ispublished": "pub", "full_text_status": "restricted", "keywords": "somite; hypaxial; limb; muscle; differentiation; signalling; embryo", "note": "\u00a9 2004 Wiley-Liss, Inc. \n\nReceived 1 September 2003; Revised 14 November 2003; Accepted 14 November 2003. \n\nThe authors thank Kim-Uyen Le and Nina Konstanian for technical support. C.P.O. and M.B.F. received funding from the NIH and C.P.O. and S.J.V. received funding from the Muscular Dystrophy Association. \n\nFunded by: NIH. Grant Numbers: RO1.AR-44483, NS41070. Muscular Dystrophy Association of America.", "abstract": "The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourqui\u00e9 et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219\u20133223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475\u20131488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.", "date": "2004-03", "date_type": "published", "publication": "Developmental Dynamics", "volume": "229", "number": "3", "publisher": "Wiley-Liss, Inc.", "pagerange": "591-599", "id_number": "CaltechAUTHORS:20160212-131244590", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-131244590", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1.AR-44483" }, { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "Muscular Dystrophy Association of America" } ] }, "doi": "10.1002/dvdy.20016", "resource_type": "article", "pub_year": "2004", "author_list": "Venters, Sara J.; Argent, Rebecca E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/zwdz9-ma266", "eprint_id": 63832, "eprint_status": "archive", "datestamp": "2023-08-22 01:33:21", "lastmod": "2023-10-17 17:12:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Cerny-R", "name": { "family": "Cerny", "given": "Robert" } }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Berger-J", "name": { "family": "Berger", "given": "J\u00fcrgen" } }, { "id": "Wilsch-Br\u00e4uninger-M", "name": { "family": "Wilsch-Br\u00e4uninger", "given": "Michaela" } }, { "id": "Kurth-T", "name": { "family": "Kurth", "given": "Thomas" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Epperlein-H-H", "name": { "family": "Epperlein", "given": "Hans-Henning" } } ] }, "title": "Combined intrinsic and extrinsic influences pattern cranial neural crest migration and pharyngeal arch morphogenesis in axolotl", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Axolotl; Head; Cranial neural crest; Migration; Branchial arches; AP-2; Snail", "note": "\u00a9 2003 Elsevier Inc. \n\nReceived for publication 10 May 2003, revised 15 August 2003, accepted 9 September 2003. Available online 4 December 2003. \n\nWe thank Tanya Moreno for the GFP mRNA, Irmin Beck for help in artwork, and Torsten Schwalm and Lewan Mtschedlischwili for technical advice. Support from NIH grant HD15527 and NASA grant NAG 2-1585 to M.B.F. and Herbert-Quandt Stiftung, SMWK, COST B-23, MSMT grant 1311004 and a NATO Science Fellowships Programme to R.C.", "abstract": "Cranial neural crest cells migrate in a precisely segmented manner to form cranial ganglia, facial skeleton and other derivatives. Here, we investigate the mechanisms underlying this patterning in the axolotl embryo using a combination of tissue culture, molecular markers, scanning electron microscopy and vital dye analysis. In vitro experiments reveal an intrinsic component to segmental migration; neural crest cells from the hindbrain segregate into distinct streams even in the absence of neighboring tissue. In vivo, separation between neural crest streams is further reinforced by tight juxtapositions that arise during early migration between epidermis and neural tube, mesoderm and endoderm. The neural crest streams are dense and compact, with the cells migrating under the epidermis and outside the paraxial and branchial arch mesoderm with which they do not mix. After entering the branchial arches, neural crest cells conduct an \"outside-in\" movement, which subsequently brings them medially around the arch core such that they gradually ensheath the arch mesoderm in a manner that has been hypothesized but not proven in zebrafish. This study, which represents the most comprehensive analysis of cranial neural crest migratory pathways in any vertebrate, suggests a dual process for patterning the cranial neural crest. Together with an intrinsic tendency to form separate streams, neural crest cells are further constrained into channels by close tissue apposition and sorting out from neighboring tissues.", "date": "2004-02-15", "date_type": "published", "publication": "Developmental Biology", "volume": "266", "number": "2", "publisher": "Elsevier", "pagerange": "252-269", "id_number": "CaltechAUTHORS:20160121-103518548", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-103518548", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD15527" }, { "agency": "NASA", "grant_number": "NAG 2-1585" }, { "agency": "Herbert-Quandt Stiftung", "grant_number": "SMWK, COST B-23" }, { "agency": "Ministry of Education, Youth and Sports of the Czech Republic (M\u0160MT)", "grant_number": "1311004" }, { "agency": "North Atlantic Treaty Organization (NATO)" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2003.09.039", "resource_type": "article", "pub_year": "2004", "author_list": "Cerny, Robert; Meulemans, Daniel; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j49c3-zv442", "eprint_id": 51959, "eprint_status": "archive", "datestamp": "2023-08-19 13:07:13", "lastmod": "2023-10-18 18:46:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Making Sense of the Sensory Lineage", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2004 American Association for the Advancement of Science.", "abstract": "Neural crest cells are multipotent stem cell-like precursors that give rise to many different cell types in the developing embryo. In her Perspective, Bronner-Fraser discusses new research ( Lee et al.) that identifies the Wnt signaling pathway as the developmental instruction directing neural crest cells to become sensory neurons.", "date": "2004-02-13", "date_type": "published", "publication": "Science", "volume": "303", "number": "5660", "publisher": "American Association for the Advancement of Science", "pagerange": "966-968", "id_number": "CaltechAUTHORS:20141119-105634787", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141119-105634787", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1126/science.1094732", "resource_type": "article", "pub_year": "2004", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wwzxx-4mg65", "eprint_id": 2355, "eprint_status": "archive", "datestamp": "2023-08-22 01:29:10", "lastmod": "2023-10-13 23:20:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A novel spalt gene expressed in branchial arches affects the ability of cranial neural crest cells to populate sensory ganglia", "ispublished": "pub", "full_text_status": "public", "keywords": "Spalt, neural crest, branchial arches, trigeminal ganglia", "note": "\"Reprinted with the permission of Cambridge University Press.\" \n\nPublished online 05 May 2004 \n\nWe thank Drs. Tatjana Sauka-Spengler and Vivian Lee for helpful comments on the manuscript. This work is supported by USPHS grants NS42287 and NS41070 to M.B.-F. We would like to thank Jhumku Kohtz for providing the pan-DLX antibody, which was made from a construct from Grace Panganiban.\n\nPublished - BARngb04.pdf
", "abstract": "Cranial neural crest cells differentiate into diverse derivatives including neurons and glia of the cranial ganglia, and cartilage and bone of the facial skeleton. Here, we explore the function of a novel transcription factor of the spalt family that might be involved in early cell-lineage decisions of the avian neural crest. The chicken spalt4 gene (csal4) is expressed in the neural tube, migrating neural crest, branchial arches and, transiently, in the cranial ectoderm. Later, it is expressed in the mesectodermal, but not neuronal or glial, derivatives of midbrain and hindbrain neural crest. After over-expression by electroporation into the cranial neural tube and neural crest, we observed a marked redistribution of electroporated neural crest cells in the vicinity of the trigeminal ganglion. In control-electroporated embryos, numerous, labeled neural crest cells ([similar]80% of the population) entered the ganglion, many of which differentiated into neurons. By contrast, few ([similar]30% of the population) spalt-electroporated neural crest cells entered the trigeminal ganglion. Instead, they localized in the mesenchyme around the ganglionic periphery or continued further ventrally to the branchial arches. Interestingly, little or no expression of differentiation markers for neurons or other cell types was observed in spalt-electroporated neural crest cells.", "date": "2004-02", "date_type": "published", "publication": "Neuron Glia Biology", "volume": "1", "number": "1", "publisher": "Cambridge University Press", "pagerange": "57-63", "id_number": "CaltechAUTHORS:BARngb04", "issn": "1740-925X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BARngb04", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS42287" }, { "agency": "NIH", "grant_number": "NS41070" } ] }, "doi": "10.1017/S1740925X04000080", "pmcid": "PMC1508165", "primary_object": { "basename": "BARngb04.pdf", "url": "https://authors.library.caltech.edu/records/wwzxx-4mg65/files/BARngb04.pdf" }, "resource_type": "article", "pub_year": "2004", "author_list": "Barembaum, Meyer and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/67n76-50475", "eprint_id": 63822, "eprint_status": "archive", "datestamp": "2023-08-22 01:15:13", "lastmod": "2023-10-17 17:11:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Amphioxus; Lamprey; Neural crest; Evolution; Id genes; Cooption", "note": "\u00a9 2003 Elsevier Inc. \n\nReceived for publication 9 May 2003, revised 5 September 2003, accepted 10 September 2003. Available online 27 October 2003. \n\nWe thank Nick Holland, Linda Holland, and John Lawrence for making amphioxus collection possible, Jim Langeland for providing us with superb cDNA libraries, and Roger Bergstedt and the staff of Hammond Bay Biological Station for assisting with lamprey embryo collection. This work was supported by USPHS grant DE13223 and NASA NAG 2-1585 to M.B.F.", "abstract": "Neural crest cells are unique to vertebrates and generate many of the adult structures that differentiate them from their closest invertebrate relatives, the cephalochordates. Id genes are robust markers of neural crest cells at all stages of development. We compared Id gene expression in amphioxus and lamprey to ask if cephalochordates deploy Id genes at the neural plate border and dorsal neural tube in a manner similar to vertebrates. Furthermore, we examined whether Id expression in these cells is a basal vertebrate trait or a derived feature of gnathostomes. We found that while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty. Furthermore, expression of lamprey Id implies that recruitment of Id genes to these cells occurred very early in the vertebrate lineage. Based on expression in amphioxus we postulate that Id cooption conferred sensory cell progenitor-like properties upon the lateral neurectoderm, and pharyngeal mesoderm-like properties upon cranial neural crest. Amphioxus Id expression is also consistent with homology between the anterior neurectoderm of amphioxus and the presumptive placodal ectoderm of vertebrates. These observations support the idea that neural crest evolution was driven in large part by cooption of multipurpose transcriptional regulators from other tissues and cell types.", "date": "2003-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "264", "number": "2", "publisher": "Elsevier", "pagerange": "430-442", "id_number": "CaltechAUTHORS:20160121-075839197", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839197", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NASA", "grant_number": "NAG 2-1585" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/j.ydbio.2003.09.006", "resource_type": "article", "pub_year": "2003", "author_list": "Meulemans, Daniel; McCauley, David; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/esjqf-gbs07", "eprint_id": 1344, "eprint_status": "archive", "datestamp": "2023-08-22 01:14:18", "lastmod": "2023-10-13 22:46:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Hemmati-H-D", "name": { "family": "Hemmati", "given": "Houman D." } }, { "id": "Nakano-Ichiro", "name": { "family": "Nakano", "given": "Ichiro" } }, { "id": "Lazareff-J-A", "name": { "family": "Lazareff", "given": "Jorge A." } }, { "id": "Masterman-Smith-M", "name": { "family": "Masterman-Smith", "given": "Michael" } }, { "id": "Geschwind-D-H", "name": { "family": "Geschwind", "given": "Daniel H." }, "orcid": "0000-0003-2896-3450" }, { "id": "Kornblum-H-I", "name": { "family": "Kornblum", "given": "Harley I." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cancerous stem cells can arise from pediatric brain tumors", "ispublished": "pub", "full_text_status": "public", "keywords": "CENTRAL-NERVOUS-SYSTEM, PROGENITOR CELLS, GENE-EXPRESSION, IDENTIFICATION, TRANSPLANTATION, MEDULLOBLASTOMA, DIFFERENTIATION, PROLIFERATION, GLIOBLASTOMA, BIOLOGY", "note": "Copyright \u00a9 2003 by the National Academy of Sciences. \n\nCommunicated by Michael E. Phelps, University of California School of Medicine, Los Angeles, CA, October 8, 2003 (received for review June 20, 2003). Published online before print November 26, 2003, 10.1073/pnas.2036535100 \n\nWe are grateful to Simon Bababeygy, Benjamin Rafii, Miguel Minera, and Alexandra Lowry for laboratory assistance and patient recruitment, Keith Tatsukawa for performing animal surgeries, Lori Shoemaker for supplying neurosphere-conditioned medium, Gary Mathern, Dennis Chute, and Beth Johnson for brain specimens, and Bud Saxton and Marcos Paiva for their guidance and support. We thank Drs. Jeffrey Twiss and Paul Mischel for helpful comments on the manuscript. This work was supported by U.S. Public Health Service Grant NS42287 (to M.B.-F.), National Institute of Mental Health Grant MH65756 (to H.I.K.), and the Jonsson Comprehensive Cancer Center at the University of California, Los Angeles. H.D.H. was supported by the McCallum Fund at California Institute of Technology, Medical Scientist Training Program Grant GM08042, and the Aesculapians Fund of the David Geffen School of Medicine at the University of California, Los Angeles.\n\nPublished - HEMpnas03.pdf
", "abstract": "Pediatric brain tumors are significant causes of morbidity and mortality. It has been hypothesized that they derive from self-renewing multipotent neural stem cells. Here, we tested whether different pediatric brain tumors, including medulloblastomas and gliomas, contain cells with properties similar to neural stem cells. We find that tumor-derived progenitors form neurospheres that can be passaged at clonal density and are able to self-renew. Under conditions promoting differentiation, individual cells are multipotent, giving rise to both neurons and glia, in proportions that reflect the tumor of origin. Unlike normal neural stem cells, however, tumor-derived progenitors have an unusual capacity to proliferate and sometimes differentiate into abnormal cells with multiple differentiation markers. Gene expression analysis reveals that both whole tumors and tumor-derived neurospheres express many genes characteristic of neural and other stem cells, including CD133, Sox2, musashi-1, bmi-1, maternal embryonic leucine zipper kinase, and phosphoserine phosphatase, with variation from tumor to tumor. After grafting to neonatal rat brains, tumor-derived neurosphere cells migrate, produce neurons and glia, and continue to proliferate for more than 4 weeks. The results show that pediatric brain tumors contain neural stem-like cells with altered characteristics that may contribute to tumorigenesis. This finding may have important implications for treatment by means of specific targeting of stem-like cells within brain tumors.", "date": "2003-12-09", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "100", "number": "25", "publisher": "National Academy of Sciences", "pagerange": "15178-15183", "id_number": "CaltechAUTHORS:HEMpnas03", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:HEMpnas03", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS42287" }, { "agency": "National Institute of Mental Health (NIMH)", "grant_number": "MH65756" }, { "agency": "Jonsson Comprehensive Cancer Center" }, { "agency": "McCallum Fellowship" }, { "agency": "NIH", "grant_number": "GM08042" }, { "agency": "Aesculapians Fund, David Geffen School of Medicine, UCLA" } ] }, "doi": "10.1073/pnas.2036535100", "pmcid": "PMC299944", "primary_object": { "basename": "HEMpnas03.pdf", "url": "https://authors.library.caltech.edu/records/esjqf-gbs07/files/HEMpnas03.pdf" }, "resource_type": "article", "pub_year": "2003", "author_list": "Hemmati, Houman D.; Nakano, Ichiro; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dnx27-vh039", "eprint_id": 27413, "eprint_status": "archive", "datestamp": "2023-08-19 12:37:07", "lastmod": "2023-10-24 17:08:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "From egg to organism", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2003 The Company of Biologists Ltd.\n\nPublished - BROdev03.pdf
", "abstract": "The embryo is a remarkable self-assembly machine. From a single cell, the fertilized egg, arises all of the differentiated cell types of the body. Embryos unfold in an elegantly choreographed manner that we strive to understand by observing the process, dissecting it into smaller bits, and mucking up the works by expressing too much or too little of some protein. Yet the mystery remains and, as knowledge and technology advance, we understand more about the depth of its complexity than about the process itself.", "date": "2003-12-01", "date_type": "published", "publication": "Development", "volume": "130", "publisher": "Company of Biologists", "pagerange": "5555-5555", "id_number": "CaltechAUTHORS:20111025-134851161", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111025-134851161", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1242/dev.00787", "primary_object": { "basename": "BROdev03.pdf", "url": "https://authors.library.caltech.edu/records/dnx27-vh039/files/BROdev03.pdf" }, "resource_type": "article", "pub_year": "2003", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/q4f93-38m13", "eprint_id": 64451, "eprint_status": "archive", "datestamp": "2023-08-22 01:11:15", "lastmod": "2023-10-17 21:24:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ahlgren-S-C", "name": { "family": "Ahlgren", "given": "Sara" } }, { "id": "Vogt-P-K", "name": { "family": "Vogt", "given": "Peter" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Excess FoxG1 causes overgrowth of the neural tube", "ispublished": "pub", "full_text_status": "restricted", "keywords": "FoxG1; chick; telencephalon; mesencephalon; cell death; growth control", "note": "\u00a9 2003 Wiley Periodicals, Inc. \n\nReceived 3 April 2003; accepted 8 June 2003. \n\nSCA was supported by an American Heart Association postdoctoral fellowship, award #1168-F11. This is TSRI Manuscript Number 15551-MEM. \n\nContract grant sponsor: American Heart Association; contract grant number: 1168-F11 (S.C.A.). \n\nContract grant sponsor: March of Dimes; contract grant number: 6-FY02-134 (S.C.A.). \n\nContract grant sponsor: NIH; contract grant number: USPHS NS36585 (M.B.F.). \n\nContract grant sponsor: NIH; contract grant number: CA79616 (P.V.).", "abstract": "The winged helix transcription factor FoxG1 (Bf-1, qin) plays multiple roles in the development of the telencephalon, with different parts of the protein affecting either proliferation or differentiation. We examined the consequences of over-expression, via retroviral expression, of FoxG1 on the growth of different regions of the chicken brain. Excess expression of FoxG1 caused a thickening of the neuroepithelium, and ultimately large outgrowths of the telencephalon and mesencephalon. In contrast, the myelencephalon appeared unaffected, exhibiting normal apoptosis and growth characteristics. A DNA binding defective form of FoxG1 did not exhibit these abnormalities, suggesting that these effects are due to FoxG1's function as a transcriptional repressor. To examine the means by which excess FoxG1caused overgrowth of the brain, we examined alterations in cell proliferation and death. No increase in proliferation was noted in any portion of the neural tube, rather a significant decrease in neuroepithelial apoptosis was seen. These results demonstrate a previously unrecognized role for winged helix factors in the regulation of neural cell apoptosis.", "date": "2003-12", "date_type": "published", "publication": "Journal of Neurobiology", "volume": "57", "number": "3", "publisher": "Wiley", "pagerange": "337-349", "id_number": "CaltechAUTHORS:20160212-082729250", "issn": "0022-3034", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160212-082729250", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "1168-F11" }, { "agency": "March of Dimes", "grant_number": "6-FY02-134" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "CA79616" } ] }, "doi": "10.1002/neu.10287", "resource_type": "article", "pub_year": "2003", "author_list": "Ahlgren, Sara; Vogt, Peter; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vszf2-rkf56", "eprint_id": 63667, "eprint_status": "archive", "datestamp": "2023-08-22 01:07:17", "lastmod": "2023-10-17 15:26:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lee-V-M", "name": { "family": "Lee", "given": "Vivian M." } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Luetolf-S", "name": { "family": "Luetolf", "given": "Simone" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Both neural crest and placode contribute to the ciliary ganglion and oculomotor nerve", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Chick; DiI; Migration; Neuronal differentiation; Neurogenesis; Ophthalmic nerve; Trigeminal ganglion", "note": "\u00a9 2003 Elsevier Inc. \n\nReceived for publication 11 March 2003, revised 28 July 2003, accepted 28 July 2003. \n\nWe thank Dr. Louis Reichardt and Dr. Virginia Lee for their generous gifts of antibodies. We thank Dr. Meyer Barenbaum for sharing his unpublished results. This work is supported by USPHS Grant NS41070 (to M.B.F.) and an American Heart Association postdoctoral fellowship (to V.M.L.).", "abstract": "The chick ciliary ganglion is a neural crest-derived parasympathetic ganglion that innervates the eye. Here, we examine its axial level of origin and developmental relationship to other ganglia and nerves of the head. Using small, focal injections of DiI, we show that neural crest cells arising from both the caudal half of the midbrain and the rostral hindbrain contribute to the ciliary as well as the trigeminal ganglion. Precursors to both ganglia have overlapping migration patterns, moving first ventrolaterally and then rostrally toward the optic vesicle. At the level of the midbrain/forebrain junction, precursors to the ciliary ganglion separate from the main migratory stream, turn ventromedially, and condense in the vicinity of the rostral aorta and Rathke's pouch. Ciliary neuroblasts first exit the cell cycle at early E2, prior to and during ganglionic condensation, and neurogenesis continues through E5.5. By E3, markers of neuronal differentiation begin to appear in this population. By labeling the ectoderm with DiI, we discovered a new placode, caudal to the eye and possibly contiguous to the trigeminal placode, that contributes a few early differentiating neurons to the ciliary ganglion, oculomotor nerve, and connecting branches to the ophthalmic nerve. These results suggest for the first time a dual neural crest and placodal contribution to the ciliary ganglion and associated nerves.", "date": "2003-11-15", "date_type": "published", "publication": "Developmental Biology", "volume": "263", "number": "2", "publisher": "Elsevier", "pagerange": "176-190", "id_number": "CaltechAUTHORS:20160114-071950620", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160114-071950620", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "American Heart Association" } ] }, "doi": "10.1016/j.ydbio.2003.07.004", "resource_type": "article", "pub_year": "2003", "author_list": "Lee, Vivian M.; Sechrist, John W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4cbq5-svq13", "eprint_id": 56440, "eprint_status": "archive", "datestamp": "2023-09-15 04:58:09", "lastmod": "2023-10-23 21:12:12", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest specification: migrating into genomics", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2015 Macmillan Publishers Limited. \n\nThe authors would like to thank S. Fraser, M. Garc\u00eda-Castro, V. Lee, Y. Marahrens and L. Ziemer for critical comments on the manuscript, and the Bronner-Fraser lab for insightful discussions. L.S.G. is supported by a K22 Career Transition Award from the NIH. Work in M.B.F.'s lab is supported, in part, by grants from NIH and NASA.", "abstract": "The bones in your face, the pigment in your skin and the neural circuitry that controls your digestive tract have one thing in common: they are all derived from neural crest cells. The formation of these migratory multipotent cells poses an interesting developmental problem, as neural crest cells are not a distinct cell type until they migrate away from the central nervous system. What defines the pool of cells with neural crest potential, and why do only some of these cells become migratory? New genomic approaches in chick, zebrafish and Xenopus might hold the key.", "date": "2003-10", "date_type": "published", "publication": "Nature Reviews Neuroscience", "volume": "4", "number": "10", "publisher": "Nature Publishing Group", "pagerange": "795-805", "id_number": "CaltechAUTHORS:20150407-135324343", "issn": "1471-003X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150407-135324343", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH K22 Career Transition Award" }, { "agency": "NASA" } ] }, "doi": "10.1038/nrn1219", "resource_type": "article", "pub_year": "2003", "author_list": "Gammill, Laura S. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ne680-stj69", "eprint_id": 6019, "eprint_status": "archive", "datestamp": "2023-08-22 00:44:28", "lastmod": "2023-10-16 20:01:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "De-Bellard-M-E", "name": { "family": "De Bellard", "given": "Maria Elena" } }, { "id": "Rao-Yi", "name": { "family": "Rao", "given": "Yi" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells", "ispublished": "pub", "full_text_status": "public", "keywords": "Slit2; neural crest; vagal; chemorepellent; gut", "note": "\u00a92003 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unpor ted license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nSubmitted: 13 January 2003; revised: 11 June 2003; accepted: 16 June 2003. \n\nWe thank Scott Fraser for helpful comments on the manuscript, Martin Garcia-Castro and Sara Ahlgren for help in analyzing the data, and Gustavo Gomez, Vivian Lee, and Joanne Tan-Cabugao for technical support. \n\nThis work was supported in part by a postdoctoral fellowship to M.E. De Bellard from the National Multiple Sclerosis Society (FA 1383-A-1) and by a United States Public Health Service grant (HD-15527) to M. Bronner-Fraser. \n\nThe supplemental material (Fig. S1 and Videos 1 and 2) is available at http://www.jcb.org/cgi/content/full/jcb.200301041/DC1. The movies show neural crest cells moving from three different experiments combined together for control and Slit2-exposed neural crest cells. The lapsed time is ~2.5 h. \n\nVideos 1 (4.2 MB) and 2 (8.3 MB) -- Slit2 enhances neural crest cell migration. Trunk neural tubes were cultured overnight on fibronectin. Cells were vitally labeled with CalceinAM (Molecular Probes) for confocal visualization after media was changed to one conditioned by control (HEK293 cells) (Video 2) or Slit2-secreting cells (Video 1) 1 h before video microscopy in a 410 LSM confocal for 3 h. Movies show three different experiments combined together for control and Slit2-exposed neural crest cells.\n\nPublished - BELjcb03.pdf
Supplemental Material - BELjcb03SF1.jpg
Supplemental Material - BELjcb03suppmovie1.MOV
Supplemental Material - BELjcb03suppmovie2.mov
", "abstract": "Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.", "date": "2003-07-21", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "162", "number": "2", "publisher": "Rockefeller University Press", "pagerange": "269-279", "id_number": "CaltechAUTHORS:BELjcb03", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BELjcb03", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Multiple Sclerosis Society", "grant_number": "FA 1383-A-1" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" } ] }, "doi": "10.1083/jcb.200301041", "pmcid": "PMC2172792", "primary_object": { "basename": "small.png", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/small.png" }, "related_objects": [ { "basename": "BELjcb03.pdf", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/BELjcb03.pdf" }, { "basename": "BELjcb03SF1.jpg", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/BELjcb03SF1.jpg" }, { "basename": "BELjcb03suppmovie1.MOV", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/BELjcb03suppmovie1.MOV" }, { "basename": "BELjcb03suppmovie2.mov", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/BELjcb03suppmovie2.mov" }, { "basename": "medium.png", "url": "https://authors.library.caltech.edu/records/ne680-stj69/files/medium.png" } ], "resource_type": "article", "pub_year": "2003", "author_list": "De Bellard, Maria Elena; Rao, Yi; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/d439k-0s553", "eprint_id": 12676, "eprint_status": "archive", "datestamp": "2023-08-22 00:35:46", "lastmod": "2023-10-17 20:25:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest contributions to the lamprey head", "ispublished": "pub", "full_text_status": "public", "keywords": "Cranial neural crest, Lamprey, DiI", "note": "Copyright \u00a9 2003 The Company of Biologists Limited. \n\nAccepted 24 February 2003. \n\nWe thank Roger Bergstedt and the staff at Hammond Bay Biological Station for facilities and assistance in lamprey collection and embryo culture; Robert Cerny for assistance with vibratome sectioning; Burcu Babaoglan for assistance with DiI labeling experiments; and Daniel Meulemans for many useful discussions. We also thank two anonymous reviewers for critical comments to improve this manuscript. This work was supported by NASA grant NAG 2-1585 to MBF and NIH training grant 5T32HD07257 to D.M.\n\nPublished - MCCdev03.pdf
", "abstract": "The neural crest is a vertebrate-specific cell population that contributes to the facial skeleton and other derivatives. We have performed focal DiI injection into the cranial neural tube of the developing lamprey in order to follow the migratory pathways of discrete groups of cells from origin to destination and to compare neural crest migratory pathways in a basal vertebrate to those of gnathostomes. The results show that the general pathways of cranial neural crest migration are conserved throughout the vertebrates, with cells migrating in streams analogous to the mandibular and hyoid streams. Caudal branchial neural crest cells migrate ventrally as a sheet of cells from the hindbrain and super-pharyngeal region of the neural tube and form a cylinder surrounding a core of mesoderm in each pharyngeal arch, similar to that seen in zebrafish and axolotl. In addition to these similarities, we also uncovered important differences. Migration into the presumptive caudal branchial arches of the lamprey involves both rostral and caudal movements of neural crest cells that have not been described in gnathostomes, suggesting that barriers that constrain rostrocaudal movement of cranial neural crest cells may have arisen after the agnathan/gnathostome split. Accordingly, neural crest cells from a single axial level contributed to multiple arches and there was extensive mixing between populations. There was no apparent filling of neural crest derivatives in a ventral-to-dorsal order, as has been observed in higher vertebrates, nor did we find evidence of a neural crest contribution to cranial sensory ganglia. These results suggest that migratory constraints and additional neural crest derivatives arose later in gnathostome evolution.", "date": "2003-06-01", "date_type": "published", "publication": "Development", "volume": "130", "number": "11", "publisher": "Company of Biologists", "pagerange": "2317-2327", "id_number": "CaltechAUTHORS:MCCdev03", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:MCCdev03", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NASA", "grant_number": "NAG 2-1585" }, { "agency": "NIH", "grant_number": "5T32HD07257" } ] }, "doi": "10.1242/dev.00451", "primary_object": { "basename": "MCCdev03.pdf", "url": "https://authors.library.caltech.edu/records/d439k-0s553/files/MCCdev03.pdf" }, "resource_type": "article", "pub_year": "2003", "author_list": "McCauley, David W. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w5rdb-k9v15", "eprint_id": 63638, "eprint_status": "archive", "datestamp": "2023-08-22 00:27:21", "lastmod": "2023-10-17 15:23:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Schubert-M", "name": { "family": "Schubert", "given": "Michael" } }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Holland-L-Z", "name": { "family": "Holland", "given": "Linda Z." } }, { "id": "Holland-N-D", "name": { "family": "Holland", "given": "Nicholas D." } } ] }, "title": "Differential mesodermal expression of two amphioxus MyoD family members (AmphiMRF1 and AmphiMRF2)", "ispublished": "pub", "full_text_status": "restricted", "keywords": "BMD1; BMD2; Branchiostoma floridae; Cephalochordate; Independent gene duplication; Muscle; Myogenesis; Somite; Transcription factor; MyoD family; MRF; MRF4; Myf5; MyoD; Myogenin", "note": "\u00a9 2003 Elsevier Science B.V. \n\nReceived 9 October 2002; received in revised form 11 December 2002; accepted 13 December 2002. \n\nThe authors are indebted to John M. Lawrence at the University of South Florida for laboratory facilities, to Jim A. Langeland for the amphioxus cDNA library and to Marc Robinson-Rechavi for invaluable statistical advice. In addition, the manuscript was improved by the constructive criticisms of Vincent Laudet, Hector Escriva Garcia, Be\u00e1trice Horard, and Pierre-Luc Bardet. This work was supported by a postdoctoral fellowship from the German Academic Exchange Service (DAAD) to M.S., by NSF grant IBN0078599 to N.D.H. and L.Z.H., and by NASA grant 98-HEDS-02 to M.B.F. and NAG2-1376 to L.Z.H.", "abstract": "To explore the evolution of myogenic regulatory factors in chordates, we isolated two MyoD family genes (AmphiMRF1 and AmphiMRF2) from amphioxus. AmphiMRF1 is first expressed at the late gastrula in the paraxial mesoderm. As the first somites form, expression is restricted to their myotomal region. In the early larva, expression is strongest in the most anterior and most posterior somites. AmphiMRF2 transcription begins at mid/late gastrula in the paraxial mesoderm, but never spreads into its most anterior region. Through much of the neurula stage, AmphiMRF2 expression is strong in the myotomal region of all somites except the most anterior pair; by late neurula expression is downregulated except in the most posterior somites forming just rostral to the tail bud. These two MRF genes of amphioxus have partly overlapping patterns of mesodermal expression and evidently duplicated independent of the diversification of the vertebrate MRF family.", "date": "2003-05", "date_type": "published", "publication": "Gene Expression Patterns", "volume": "3", "number": "2", "publisher": "Elsevier", "pagerange": "199-202", "id_number": "CaltechAUTHORS:20160113-122350057", "issn": "1567-133X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160113-122350057", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Deutscher Akademischer Austauschdienst (DAAD)" }, { "agency": "NSF", "grant_number": "IBN0078599" }, { "agency": "NASA", "grant_number": "98-HEDS-02" }, { "agency": "NASA", "grant_number": "NAG2-1376" } ] }, "doi": "10.1016/S1567-133X(02)00099-6", "resource_type": "article", "pub_year": "2003", "author_list": "Schubert, Michael; Meulemans, Daniel; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hb3rg-wsf23", "eprint_id": 27304, "eprint_status": "archive", "datestamp": "2023-08-21 23:54:10", "lastmod": "2023-10-24 17:03:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Gammill-L-S", "name": { "family": "Gammill", "given": "Laura S." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Genomic analysis of neural crest induction", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; induction; migration; macroarray; subtraction; chick", "note": "\u00a9 2002 The Company of Biologists Ltd.\n\nAccepted 27 September 2002.\n\nSpecial thanks to Constanza Gonzalez for her expert technical\nassistance. We gratefully acknowledge Jonathan Rast and Cristina\nCalestani for patiently answering our many questions about\nmacroarray screening. We thank Yun Kee and Carole LaBonne for\nuseful discussions, and Scott Fraser, Andrew Groves and York\nMarahrens for critical reading of the manuscript. We are particularly\ngrateful to Titus Brown for helpful discussions and aiding with the\nbioinformatics analysis. L. S. G. was supported by an NICHD NRSA\nIndividual Postdoctoral Fellowship. This work was supported by\nUSPHS NS36585.\n\nPublished - GAMdev02.pdf
", "abstract": "The vertebrate neural crest is a migratory stem cell population that arises within the central nervous system. Here, we combine embryological techniques with array technology to describe 83 genes that provide the first gene expression profile of a newly induced neural crest cell. This profile contains numerous novel markers of neural crest precursors and reveals previously unrecognized similarities between neural crest cells and endothelial cells, another migratory cell population. We have performed a secondary screen using in situ hybridization that allows us to extract temporal information and reconstruct the progression of neural crest gene expression as these cells become different from their neighbors and migrate. Our results reveal a sequential `migration activation' process that reflects stages in the transition to a migratory neural crest cell and suggests that migratory potential is established in a pool of cells from which a subset are activated to migrate.", "date": "2002-12", "date_type": "published", "publication": "Development", "volume": "129", "number": "24", "publisher": "Company of Biologists", "pagerange": "5731-5741", "id_number": "CaltechAUTHORS:20111019-104512170", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111019-104512170", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Institute of Child Health and Human Development (NICHD)" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" }, { "agency": "NIH Postdoctoral Fellowship" } ] }, "doi": "10.1242/dev.00175", "primary_object": { "basename": "GAMdev02.pdf", "url": "https://authors.library.caltech.edu/records/hb3rg-wsf23/files/GAMdev02.pdf" }, "resource_type": "article", "pub_year": "2002", "author_list": "Gammill, Laura S. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ex09v-kfz97", "eprint_id": 63824, "eprint_status": "archive", "datestamp": "2023-08-21 23:54:38", "lastmod": "2023-10-17 17:11:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lee-V-M", "name": { "family": "Lee", "given": "Vivian M." } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Nishi-R", "name": { "family": "Nishi", "given": "Rae" } } ] }, "title": "Neuronal Differentiation from Postmitotic Precursors in the Ciliary Ganglion", "ispublished": "pub", "full_text_status": "restricted", "keywords": "ciliary ganglion; parasympathetic; neuronal differentiation; quail; Islet-1; HuD; transplantation; neurogenesis; neural crest", "note": "\u00a9 2002 Elsevier Science. \n\nReceived for publication December 28, 2001. Revised October 11, 2002. Accepted October 14, 2002. Published online November 19, 2002. \n\nWe thank Greg Smiley and Fanny Vang for technical assistance. We are also grateful to Dr. Jim Weston and Dr. Mike Marusich for their generous gifts of antibodies. We thank Dr. Lou Reichardt for supplying us with the anti-p75 neurotrophin receptor. We are also indebted to Dr. Gary Banker and his laboratory for the use of their imaging setup. We also thank Dan Darcy at the Caltech BioImaging\nCenter for assistance and training of confocal microscopy. This study was funded by NS25767 (to R.N.), NS41070 (to J.W.S. and M.B.F.), and N. L. Tartar fellowship (to V.M.L.).", "abstract": "In the chick ciliary ganglion, neuronal number is kept constant between St. 29 and St. 34 (E6\u2013E8) despite a large amount of cell death. Here, we characterize the source of neurogenic cells in the ganglion as undifferentiated neural crest-derived cells. At St. 29, neurons and nonneuronal cells in the ciliary ganglion expressed the neural crest markers HNK-1 and p75^(NTR). Over 50% of the cells were neurons at St. 29; of the nonneuronal cells, a small population expressed glial markers, whereas the majority was undifferentiated. When placed in culture, nonneuronal cells acquired immunoreactivity for HuD, suggesting that they had commenced neuronal differentiation. The newly differentiated neurons arose from precursors that did not incorporate bromodeoxyuridine. To test whether these precursors could undergo neural differentiation in vivo, purified nonneuronal cells from St. 29 quail ganglia were transplanted into chick embryos at St. 9\u201314. Subsequently, quail cells expressing neuronal markers were found in the chick ciliary ganglion. The existence of this precursor pool was transient because nonneuronal cells isolated from St. 38 ganglia failed to form neurons. Since all ciliary ganglion neurons are born prior to St. 29, these results demonstrate that there are postmitotic neural crest-derived precursors in the developing ciliary ganglion that can differentiate into neurons in the appropriate environment.", "date": "2002-12", "date_type": "published", "publication": "Developmental Biology", "volume": "252", "number": "2", "publisher": "Elsevier", "pagerange": "312-323", "id_number": "CaltechAUTHORS:20160121-075839812", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839812", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS25767" }, { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "N. L. Tartar Fellowship" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1006/dbio.2002.0882", "resource_type": "article", "pub_year": "2002", "author_list": "Lee, Vivian M.; Sechrist, John W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8je4t-74e85", "eprint_id": 12897, "eprint_status": "archive", "datestamp": "2023-08-19 10:15:13", "lastmod": "2023-10-17 21:13:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Amphioxus and lamprey AP-2 genes: implications for neural crest evolution and migration patterns", "ispublished": "pub", "full_text_status": "public", "keywords": "AP-2, Amphioxus, Lamprey, Neural crest, Migration", "note": "Copyright \u00a9 2002 The Company of Biologists Limited. \n\nAccepted 30 July 2002. \n\nWe thank Linda and Nick Holland for making amphioxus collection possible, John Lawrence for laboratory space in Tampa, Jim Langeland for generously providing excellent cDNA libraries, David McCauley for supplying lamprey embryos, and Robert Cerny for invaluable discussion. This work was supported by NASA grant 98-HEDS-02 to M.B.-F.\n\nPublished - MEUdev02.pdf
", "abstract": "The neural crest is a uniquely vertebrate cell type present in the most basal vertebrates, but not in cephalochordates. We have studied differences in regulation of the neural crest marker AP-2 across two evolutionary transitions: invertebrate to vertebrate, and agnathan to gnathostome. Isolation and comparison of amphioxus, lamprey and axolotl AP-2 reveals its extensive expansion in the vertebrate dorsal neural tube and pharyngeal arches, implying co-option of AP-2 genes by neural crest cells early in vertebrate evolution. Expression in non-neural ectoderm is a conserved feature in amphioxus and vertebrates, suggesting an ancient role for AP-2 genes in this tissue. There is also common expression in subsets of ventrolateral neurons in the anterior neural tube, consistent with a primitive role in brain development. Comparison of AP-2 expression in axolotl and lamprey suggests an elaboration of cranial neural crest patterning in gnathostomes. However, migration of AP-2-expressing neural crest cells medial to the pharyngeal arch mesoderm appears to be a primitive feature retained in all vertebrates. Because AP-2 has essential roles in cranial neural crest differentiation and proliferation, the co-option of AP-2 by neural crest cells in the vertebrate lineage was a potentially crucial event in vertebrate evolution.", "date": "2002-11-01", "date_type": "published", "publication": "Development", "volume": "129", "number": "21", "publisher": "Company of Biologists", "pagerange": "4953-4962", "id_number": "CaltechAUTHORS:MEUdev02", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:MEUdev02", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NASA", "grant_number": "98-HEDS-02" } ] }, "primary_object": { "basename": "MEUdev02.pdf", "url": "https://authors.library.caltech.edu/records/8je4t-74e85/files/MEUdev02.pdf" }, "resource_type": "article", "pub_year": "2002", "author_list": "Meulemans, Daniel and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4et68-xy961", "eprint_id": 63895, "eprint_status": "archive", "datestamp": "2023-08-21 23:50:19", "lastmod": "2023-10-17 17:16:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Moreno-T-A", "name": { "family": "Moreno", "given": "Tanya A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural expression of mouse Noelin-1/2 and comparison with other vertebrates", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Mouse; Noelin; Neural crest; Neural plate; Cranial placodes; Cranial ganglia", "note": "\u00a9 2002 Elsevier Science Ireland Ltd. \n\nReceived 11 April 2002; received in revised form 31 May 2002; accepted 31 May 2002. \n\nThe authors wish to thank Anne Knecht and Clare Baker for reading the manuscript and Meyer Barembaum for providing photographs of chick Noelin-1/2 expression for Fig. 4. T.A.M. is a Fellow of the ARCS foundation. This work was supported by grant NS42287 to M.B.-F.", "abstract": "Noelins are secreted glycoproteins with important developmental functions in frogs and birds. Here, we present the expression pattern of the mouse homolog of Noelin-1/2 at E8\u201310 of development and compare this pattern to other vertebrates. Expression was observed in the neural plate and neural crest, as well as in the cranial ganglia. Later, expression is prominent in brain tissue and in the zone of polarizing activity in the limb.", "date": "2002-11", "date_type": "published", "publication": "Mechanisms of Development", "volume": "119", "number": "1", "publisher": "Elsevier", "pagerange": "121-125", "id_number": "CaltechAUTHORS:20160122-105357540", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160122-105357540", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS42287" }, { "agency": "ARCS Foundation" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/S0925-4773(02)00308-8", "resource_type": "article", "pub_year": "2002", "author_list": "Moreno, Tanya A. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tv2k2-j1m33", "eprint_id": 63823, "eprint_status": "archive", "datestamp": "2023-08-19 10:02:53", "lastmod": "2023-10-17 17:11:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Stark-M-R", "name": { "family": "Stark", "given": "Michael R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Pax3-Expressing Trigeminal Placode Cells Can Localize to Trunk Neural Crest Sites but Are Committed to a Cutaneous Sensory Neuron Fate", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2002 Elsevier Science. \n\nReceived for publication May 6, 2002. Revised June 24, 2002. Published online August 13, 2002. \n\nWe thank Dr. Louis Reichardt for the anti-TrkB antibody and Dr. Hideaki Tanaka for the QN antibody. Thanks to Drs. Andy Groves and Roger Keynes for valuable comments on the manuscript. This work was supported by NS-041070\u201302 from the NIH (to M.B.-F.) and by Human Frontier Science Program Fellowship LT-63/96 and American Heart Association Fellowships 1171-FI1 and 0020097Y (to C.V.H.B.).", "abstract": "The cutaneous sensory neurons of the ophthalmic lobe of the trigeminal ganglion are derived from two embryonic cell populations, the neural crest and the paired ophthalmic trigeminal (opV) placodes. Pax3 is the earliest known marker of opV placode ectoderm in the chick. Pax3 is also expressed transiently by neural crest cells as they emigrate from the neural tube, and it is reexpressed in neural crest cells as they condense to form dorsal root ganglia and certain cranial ganglia, including the trigeminal ganglion. Here, we examined whether Pax3+ opV placode-derived cells behave like Pax3+ neural crest cells when they are grafted into the trunk. Pax3+ quail opV ectoderm cells associate with host neural crest migratory streams and form Pax3+ neurons that populate the dorsal root and sympathetic ganglia and several ectopic sites, including the ventral root. Pax3 expression is subsequently downregulated, and at E8, all opV ectoderm-derived neurons in all locations are large in diameter, and virtually all express TrkB. At least some of these neurons project to the lateral region of the dorsal horn, and peripheral quail neurites are seen in the dermis, suggesting that they are cutaneous sensory neurons. Hence, although they are able to incorporate into neural crest-derived ganglia in the trunk, Pax3+ opV ectoderm cells are committed to forming cutaneous sensory neurons, their normal fate in the trigeminal ganglion. In contrast, Pax3 is not expressed in neural crest-derived neurons in the dorsal root and trigeminal ganglia at any stage, suggesting either that Pax3 is expressed in glial cells or that it is completely downregulated before neuronal differentiation. Since Pax3 is maintained in opV placode-derived neurons for some considerable time after neuronal differentiation, these data suggest that Pax3 may play different roles in opV placode cells and neural crest cells.", "date": "2002-09-15", "date_type": "published", "publication": "Developmental Biology", "volume": "249", "number": "2", "publisher": "Elsevier", "pagerange": "219-236", "id_number": "CaltechAUTHORS:20160121-075839528", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-075839528", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS-041070\u201302" }, { "agency": "Human Frontier Science Program", "grant_number": "LT-63/96" }, { "agency": "American Heart Association", "grant_number": "1171-FI1" }, { "agency": "American Heart Association", "grant_number": "0020097Y" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1006/dbio.2002.0767", "resource_type": "article", "pub_year": "2002", "author_list": "Baker, Clare V. H.; Stark, Michael R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/c6x7q-57t18", "eprint_id": 63816, "eprint_status": "archive", "datestamp": "2023-08-19 10:01:21", "lastmod": "2023-10-17 17:11:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "De-Bellard-M-E", "name": { "family": "De Bellard", "given": "Maria Elena" } }, { "id": "Ching-Wendy", "name": { "family": "Ching", "given": "Wendy" } }, { "id": "Gossler-A", "name": { "family": "Gossler", "given": "Achim" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Disruption of Segmental Neural Crest Migration and Ephrin Expression in Delta-1 Null Mice", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2002 Elsevier Science. \n\nReceived for publication April 11, 2002. Revised May 17, 2002. Accepted May 21, 2002. Published online July 31, 2002. \n\nWe thank Gustavo Gomez for excellent technical assistance. This work was supported by USPHS, HD-15527 NS-41070 and Human Frontiers Grant RG0146 (to M.B.-F.) with a minority supplement to MEdB on HD-15527. We thank Dr. David J. Anderson (Caltech) for Sox10, ephrin and Eph cDNA probes, and Dr. Rusty Lansford (Caltech) for ephrinB1- and ephrinA5-Fc.", "abstract": "Neural crest cells migrate segmentally through the rostral half of each trunk somite due to inhibitory influences of ephrins and other molecules present in the caudal-half of somites. To examine the potential role of Notch/Delta signaling in establishing the segmental distribution of ephrins, we examined neural crest migration and ephrin expression in Delta-1 mutant mice. Using Sox-10 as a marker, we noted that neural crest cells moved through both rostral and caudal halves of the somites in mutants, consistent with the finding that ephrinB2 levels are significantly reduced in the caudal-half somites. Later, mutant embryos had aberrantly fused and/or reduced dorsal root and sympathetic ganglia, with a marked diminution in peripheral glia. These results show that Delta-1 is essential for proper migration and differentiation of neural crest cells. Interestingly, absence of Delta-1 leads to diminution of both neurons and glia in peripheral ganglia, suggesting a general depletion of the ganglion precursor pool in mutant mice.", "date": "2002-09-01", "date_type": "published", "publication": "Developmental Biology", "volume": "249", "number": "1", "publisher": "Elsevier", "pagerange": "121-130", "id_number": "CaltechAUTHORS:20160121-071017262", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160121-071017262", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS-41070" }, { "agency": "Human Frontier Science Program", "grant_number": "RG0146" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1006/dbio.2002.0756", "resource_type": "article", "pub_year": "2002", "author_list": "De Bellard, Maria Elena; Ching, Wendy; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jddwe-ft726", "eprint_id": 4928, "eprint_status": "archive", "datestamp": "2023-08-21 23:36:27", "lastmod": "2023-10-16 18:00:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ahlgren-S-C", "name": { "family": "Ahlgren", "given": "Sara C." } }, { "id": "Thakur-V", "name": { "family": "Thakur", "given": "Vijaya" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Sonic hedgehog rescues cranial neural crest from cell death induced by ethanol exposure", "ispublished": "pub", "full_text_status": "public", "note": "Copyright \u00a9 2002 by the National Academy of Sciences \n\nCommunicated by Eric H. Davidson, California Institute of Technology, Pasadena, CA and approved June 14, 2002 (received for review March 14, 2002) \n\nWe thank Sarah Mahoney for technical assistance, Laura Gammill and Tanya Moreno for advice, and Clare Baker and Martin Garcia-Castro for reading the manuscript. We extend many thanks to Drs. Cliff Tabin, Juan Carlos Izpisua-Belmonte, and Mary Dickinson for materials. S.C.A. was supported by an American Heart Association fellowship, Award 1168-F11, as well as by National Institutes of Health Grant USPHS NS36585, awarded to M.B.-F.\n\nPublished - AHLpnas02.pdf
", "abstract": "Alcohol is a teratogen that induces a variety of abnormalities including brain and facial defects [Jones, K. & Smith, D. (1973) Lancet 2, 999-1001], with the exact nature of the deficit depending on the time and magnitude of the dose of ethanol to which developing fetuses are exposed. In addition to abnormal facial structures, ethanol-treated embryos exhibit a highly characteristic pattern of cell death. Dying cells are observed in the premigratory and migratory neural crest cells that normally populate most facial structures. The observation that blocking Sonic hedgehog (Shh) signaling results in similar craniofacial abnormalities prompted us to examine whether there was a link between this aspect of fetal alcohol syndrome and loss of Shh. We demonstrate that administration of ethanol to chick embryos results in a dramatic loss of Shh, as well as a loss of transcripts involved in Shh signaling pathways. In contrast, other signaling molecules examined do not demonstrate such dramatic changes. Furthermore, we demonstrate that both the ethanol-induced cranial neural crest cell death and the associated craniofacial growth defect can be rescued by application of Shh. These data suggest that craniofacial anomalies resulting from fetal alcohol exposure are caused at least partially by loss of Shh and subsequent neural crest cell death.", "date": "2002-08-06", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "99", "number": "16", "publisher": "National Academy of Sciences", "pagerange": "10476-10481", "id_number": "CaltechAUTHORS:AHLpnas02", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:AHLpnas02", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "1168-F11" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1073/pnas.162356199", "pmcid": "PMC124946", "primary_object": { "basename": "AHLpnas02.pdf", "url": "https://authors.library.caltech.edu/records/jddwe-ft726/files/AHLpnas02.pdf" }, "resource_type": "article", "pub_year": "2002", "author_list": "Ahlgren, Sara C.; Thakur, Vijaya; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/623rj-69q12", "eprint_id": 51911, "eprint_status": "archive", "datestamp": "2023-08-19 09:54:03", "lastmod": "2023-10-18 18:41:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Mart\u00edn I." } }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Ectodermal Wnt Function as a Neural Crest Inducer", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 2002 American Association for the Advancement of Science.\n\n12 February 2002; accepted 28 May 2002; published online 13 June 2002.\n\nSupported by NIH grant number NS36585. We thank C. Baker, A. Groves, and A. Knecht for helpful comments on this work.\n\nSupplemental Material - 1070824S1_large.jpg
Supplemental Material - 1070824S2_large.jpg
", "abstract": "Neural crest cells, which generate peripheral nervous system and facial skeleton, arise at the neural plate/ectodermal border via an inductive interaction between these tissues. Wnts and bone morphogenetic proteins (BMPs) play roles in neural crest induction in amphibians and zebrafish. Here, we show that, in avians, Wnt6 is localized in ectoderm and in vivo inhibition of Wnt signaling perturbs neural crest formation. Furthermore, Wnts induce neural crest from na\u0131\u0308ve neural plates in vitro in a defined medium without added factors, whereas BMPs require additives. Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos.", "date": "2002-08-02", "date_type": "published", "publication": "Science", "volume": "297", "number": "5582", "publisher": "American Association for the Advancement of Science", "pagerange": "848-851", "id_number": "CaltechAUTHORS:20141118-131539860", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141118-131539860", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1126/science.1070824", "primary_object": { "basename": "1070824S1_large.jpg", "url": "https://authors.library.caltech.edu/records/623rj-69q12/files/1070824S1_large.jpg" }, "related_objects": [ { "basename": "1070824S2_large.jpg", "url": "https://authors.library.caltech.edu/records/623rj-69q12/files/1070824S2_large.jpg" } ], "resource_type": "article", "pub_year": "2002", "author_list": "Garc\u00eda-Castro, Mart\u00edn I.; Marcelle, Christophe; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rrkad-6yk91", "eprint_id": 47414, "eprint_status": "archive", "datestamp": "2023-08-21 23:29:22", "lastmod": "2023-10-26 20:34:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Peters-John", "name": { "family": "Peters", "given": "John" } }, { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "Jack" } }, { "id": "Luetolf-S", "name": { "family": "Luetolf", "given": "Simone" } }, { "id": "Loredo-G", "name": { "family": "Loredo", "given": "Grace" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Spatial Expression of the Alternatively Spliced EIIIB and EIIIA Segments of Fibronectin in the Early Chicken Embryo", "ispublished": "pub", "full_text_status": "restricted", "keywords": "alternative splicing; chicken embryo; development; extracellular matrix; fibronectin; neural crest", "note": "\u00a9 2002 Taylor and Francis.\n\nReceived 29 July 2002; accepted 26 November 2002.\n\nJHP was supported by a UCLA Claude Pepper\nOlder Americans Independence Center, NIA #P60\nAG10415, a gift from the Charles B. See Foundation,\nas well as Career Development Award and Merit Review\nGrants, both from the Department of Veterans\nAffairs. MBF was supported by USPHS HD15527.\nWe wish to thank Richard Hynes for generous gifts\nof antibodies.", "abstract": "Using domain-specific antibodies, we have analyzed the tissue distribution of fibronectins\n(FNs) containing the alternatively spliced EIIIB and EIIIA segments relative to total FN in early\nchicken embryos. The results show a selective loss of EIIIA+ FN staining in the notochordal\nsheath and in cartilaginous structures between 4.5 and 7.0 days of development. In other regions,\nEIIIB+ and EIIIA+ FNs are extensively codistributed in and around mesoderm-derived\nstructures (somites, notochord, heart, and blood vessels), in basal laminae of endoderm and\nectoderm-derived structures, as well as within the vicinity of neural crest formation and migration.\nWe also noted that EIIIA staining overlaps with spatial patterns of distribution that have\npreviously been described for the \u03b14 integrin subunit, a component of the EIIIA receptor \u03b14\u03b21.", "date": "2002-07", "date_type": "published", "publication": "Cell Communication and Adhesion", "volume": "9", "number": "4", "publisher": "Informa Healthcare", "pagerange": "221-238", "id_number": "CaltechAUTHORS:20140723-083018918", "issn": "1541-9061", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20140723-083018918", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "UCLA Claude Pepper Older Americans Independence Center" }, { "agency": "NIH", "grant_number": "P60 AG10415" }, { "agency": "Charles B. See Foundation" }, { "agency": "Department of Veterans Affairs" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD15527" } ] }, "doi": "10.1080/15419060216015", "resource_type": "article", "pub_year": "2002", "author_list": "Peters, John; Sechrist, Jack; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/e48ze-qax02", "eprint_id": 56496, "eprint_status": "archive", "datestamp": "2023-08-19 09:35:07", "lastmod": "2023-10-23 15:29:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Knecht-A-K", "name": { "family": "Knecht", "given": "Anne K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Induction of the neural crest: a multigene process", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2002 Nature Publishing Group. \n\nThe authors thank T. Moreno for help with the figures, and M. Garc\u00eda-Castro and M. Albrecht for critical reading of the manuscript.", "abstract": "In the embryo, the neural crest is an important population of cells that gives rise to diverse derivatives, including the peripheral nervous system and the craniofacial skeleton. Evolutionarily, the neural crest is of interest as an important innovation in vertebrates. Experimentally, it represents an excellent system for studying fundamental developmental processes, such as tissue induction. Classical embryologists have identified interactions between tissues that lead to neural crest formation. More recently, geneticists and molecular biologists have identified the genes that are involved in these interactions; this recent work has revealed that induction of the neural crest is a complex multistep process that involves many genes.", "date": "2002-06", "date_type": "published", "publication": "Nature Reviews. Genetics", "volume": "3", "number": "6", "publisher": "Nature Publishing Group", "pagerange": "453-461", "id_number": "CaltechAUTHORS:20150408-145638064", "issn": "1471-0056", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150408-145638064", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1038/nrg819", "resource_type": "article", "pub_year": "2002", "author_list": "Knecht, Anne K. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vqgvv-9xf52", "eprint_id": 64942, "eprint_status": "archive", "datestamp": "2023-08-21 23:13:12", "lastmod": "2023-10-17 22:00:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McCauley-D-W", "name": { "family": "McCauley", "given": "David W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Conservation of Pax gene expression in ectodermal placodes of the lamprey", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Ectodermal placode; Gene expression; Pax-7; Pax-2; Conservation", "note": "\u00a9 2002 Elsevier Science B.V. \n\nReceived 27 August 2001; received in revised form 4 December 2001; accepted 12 December 2001. Available online 15 March 2002. Received by R. Di Lauro. \n\nWe thank Roger Bergstedt and the staff of Hammond Bay Biological Station and Dr. Jim Langeland for assistance in collection of embryos, Burcu Babaoglan for assistance with LampPax-2 in situs and library screening, and Dan Meulemans for excellent technical assistance. This work was supported by NASA grant 98-HEDS-02 to MBF.", "abstract": "Ectodermal placodes contribute to the cranial ganglia and sense organs of the head and, together with neural crest cells, represent defining features of the vertebrate embryo. The identity of different placodes appears to be specified in part by the expression of different Pax genes, with Pax-3/7 class genes being expressed in the trigeminal placode of mice, chick, frogs and fish, and Pax-2/5/8 class genes expressed in the otic placode. Here, we present the cloning and expression pattern of lamprey Pax-7 and Pax-2, which mark the trigeminal and otic placodes, respectively, as well as other structures characteristic of vertebrate Pax genes. These results suggest conservation of Pax genes and placodal structures in basal and derived vertebrates.", "date": "2002-04-03", "date_type": "published", "publication": "Gene", "volume": "287", "number": "1-2", "publisher": "Elsevier", "pagerange": "129-139", "id_number": "CaltechAUTHORS:20160302-071129792", "issn": "0378-1119", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-071129792", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NASA", "grant_number": "98-HEDS-02" } ] }, "doi": "10.1016/S0378-1119(01)00894-0", "resource_type": "article", "pub_year": "2002", "author_list": "McCauley, David W. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8bh61-xbd27", "eprint_id": 63683, "eprint_status": "archive", "datestamp": "2023-08-21 23:09:25", "lastmod": "2023-10-17 15:27:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kious-B-M", "name": { "family": "Kious", "given": "Brent M." } }, { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Knecht-A-K", "name": { "family": "Knecht", "given": "Anne K." } } ] }, "title": "Identification and Characterization of a Calcium Channel \u03b3 Subunit Expressed in Differentiating Neurons and Myoblasts", "ispublished": "pub", "full_text_status": "restricted", "keywords": "CACNG4 (\u03b34); calcium channel; neuronal differentiation; myoblasts; cranial ganglia; placodes", "note": "\u00a9 2002 Elsevier Science (USA). \n\nReceived for publication September 14, 2001. Revised December 4, 2001. Accepted December 4, 2001. Published online February 11, 2002. \n\nWe thank Yun Kee for the chick embryo library, Jack Sechrist for assistance with anatomical identification, and Helen McBride and Tanya Moreno for critical reading of the manuscript. This work was supported by grants from the NIH (DE13223 and NS41070) to M.B.F.; B.M.K. was supported by an undergraduate research fellowship from the Arnold and Mabel Beckman Foundation; C.V.H.B. was supported by American Heart Association Fellowships 1171-F11 and 0020097Y; and A.K.K. was supported by postdoctoral fellowships from the Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation, and from the NIH (DE14134).", "abstract": "Transient elevations of intracellular calcium (calcium transients) play critical roles in many developmental processes, including differentiation. Although the factors that regulate calcium transients are not clearly defined, calcium influx may be controlled by molecules interacting with calcium channels, including channel regulatory subunits. Here, we describe the chick \u03b34 regulatory subunit (CACNG4), the first such subunit to be characterized in early development. CACNG4 is expressed early in the cranial neural plate, and later in the cranial and dorsal root ganglia; importantly, the timing of this later expression correlates precisely with the onset of neuronal differentiation. CACNG4 expression is also observed in nonneuronal tissues undergoing differentiation, specifically the myotome and a subpopulation of differentiating myoblasts in the limb bud. Finally, within the distal cranial ganglia, we show that CACNG4 is expressed in placode-derived cells (prospective neurons), but also, surprisingly, in neural crest-derived cells, previously shown to form only glia in this location; contrary to these previous results, we find that neural crest cells can form neurons in the distal ganglia. Given the proposed role of CACNG4 in modulating calcium channels and its expression in differentiating cells, we suggest that CACNG4 may promote differentiation via regulation of intracellular calcium levels.", "date": "2002-03-15", "date_type": "published", "publication": "Developmental Biology", "volume": "243", "number": "2", "publisher": "Elsevier", "pagerange": "249-259", "id_number": "CaltechAUTHORS:20160114-101925932", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925932", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "Arnold and Mabel Beckman Foundation" }, { "agency": "American Heart Association", "grant_number": "1171-F11" }, { "agency": "American Heart Association", "grant_number": "0020097Y" }, { "agency": "Damon Runyon-Walter Winchell Foundation Cancer Research Fund" }, { "agency": "NIH", "grant_number": "DE14134" } ] }, "doi": "10.1006/dbio.2001.0570", "resource_type": "article", "pub_year": "2002", "author_list": "Kious, Brent M.; Baker, Clare V. H.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b9xqp-q2r20", "eprint_id": 57240, "eprint_status": "archive", "datestamp": "2023-08-19 08:59:58", "lastmod": "2023-10-23 17:11:47", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ahlgren-S-C", "name": { "family": "Ahlgren", "given": "Sara C." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Recycling signaling molecules during development", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2002 Nature Publishing Group.", "abstract": "Sonic hedgehog (Shh) has many functions in development. A new study reports a key role for Shh in cell survival, proliferation and morphogenesis in formation of the forebrain and midbrain.", "date": "2002-02", "date_type": "published", "publication": "Nature Neuroscience", "volume": "5", "number": "2", "publisher": "Nature Publishing Group", "pagerange": "87-88", "id_number": "CaltechAUTHORS:20150505-140745486", "issn": "1097-6256", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150505-140745486", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1038/nn0202-87", "resource_type": "article", "pub_year": "2002", "author_list": "Ahlgren, Sara C. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/gr85j-5ea03", "eprint_id": 64011, "eprint_status": "archive", "datestamp": "2023-08-21 22:55:43", "lastmod": "2023-10-17 18:34:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular analysis of neural crest formation", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural Crest; BMP; Wnt; Neural development; Neural plate; Ectoderm", "note": "\u00a9 2002 Elsevier Science Ltd. \n\nAvailable online 19 December 2001.", "abstract": "Neural crest cells arise within the ectoderm during neurulation and give rise to most of the peripheral nervous system. Following neural tube closure, they come to lie within the dorsal neural tube from which they emerge and subsequently migrate extensively to numerous and characteristic sites. There, they differentiate into neurons and glia of the peripheral nervous system, cartilage and bone of the face, melanocytes and various other cell types. Fate mapping experiments have demonstrated that the neural crest arises at the juncture between presumptive epidermis and neural plate. However, injection of lineage tracer into individual cells reveals that single neural fold cells are not committed to a neural crest fate; rather these cells can form all ectodermal derivatives (epidermis, neural tube, neural crest). Inductive interactions between the neural and non-neural ectoderm can generate neural crest cells, suggesting that signals travel through the epidermis to generate neural crest cells prior to neural tube closure. Induction of the neural crest appears to be a multiphasic process and involves a combination of an early Wnt signal together with later functions of BMP signaling pathways. We have used a variety of molecular screens to isolate molecular constituents involved in neural crest formation. We have identified a secreted factor, Noelin-1, which is expressed in the prospective avian neural plate and may play a role making the neural tube competent to form neural crest. Noelin-1 mRNA is expressed in a graded pattern in the closing neural tube, with highest expression in the neural folds and no detectable expression at the ventral midline. Its expression precedes that of Slug, a zinc finger transcription factor that represents the earliest known neural crest marker gene. Over-expression of Noelin-1 using recombinant retroviruses causes an excess of neural crest emigration and prolongs the time that the neural tube is competent to generate and regenerate neural crest cells. These results support an important role for Noelin-1 in rendering the neural tube competent to respond to inductive cues to generate neural crest.", "date": "2002-01", "date_type": "published", "publication": "Journal of Physiology - Paris", "volume": "96", "number": "1-2", "publisher": "Elsevier", "pagerange": "3-8", "id_number": "CaltechAUTHORS:20160127-111127543", "issn": "0928-4257", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160127-111127543", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0928-4257(01)00074-2", "resource_type": "article", "pub_year": "2002", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/296b6-7b834", "eprint_id": 63682, "eprint_status": "archive", "datestamp": "2023-08-21 22:48:23", "lastmod": "2023-10-17 15:27:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Moreno-T-A", "name": { "family": "Moreno", "given": "Tanya A." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The Secreted Glycoprotein Noelin-1 Promotes Neurogenesis in Xenopus", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Noelin-1; Xenopus laevis; Noggin; primary neurogenesis; cranial ganglia; XBrn-3d; NeuroD; N-tubulin; neural crest; placodes", "note": "\u00a9 2001 Elsevier Science. \n\nReceived for publication June 20, 2001. Revised September 6, 2001. Accepted September 10, 2001. Published online November 14, 2001. \n\nThe authors gratefully acknowledge Drs. A. Knecht, L. Gammill and C. Baker for critically reading the manuscript; C. Baker, L. Gammill, A. Knecht, members of the laboratory and especially C. LaBonne for support, valuable insights and discussion. C. LaBonne kindly assisted with oocyte labeling experiments. The authors thank the following for their kind gifts of reagents: Drs. R Harland for the Xenopus cDNA library; D. Anderson, X-ngnr-1; A. Br\u00e4ndli, Pax-2; K. Cho, 6-myc-Noggin; C. Kintner, N-tubulin; J. Lee, XNeuroD; R. Mayor, Xslug. The authors also thank the anonymous reviewers for helpful comments. T.A.M. is a Fellow of the ARCS Foundation. This work was supported by USPHS grants NS36585 and NS41070.", "abstract": "Neurogenesis in Xenopus neural ectoderm involves multiple gene families, including basic helix-loop-helix transcription factors, which initiate and control primary neurogenesis. Equally important, though less well understood, are the downstream effectors of the activity of these transcription factors. We have investigated the role of a candidate downstream effector, Noelin-1, during Xenopus development. Noelin-1 is a secreted glycoprotein that likely forms large multiunit complexes. In avians, overexpression of Noelin-1 causes prolonged and excessive neural crest migration. Our studies in Xenopus reveal that this gene, while highly conserved in sequence, has a divergent function in primary neurogenesis. XenopusNoelin-1 is expressed mainly by postmitotic neurogenic tissues in the developing central and peripheral nervous systems, first appearing after neural tube closure. Its expression is upregulated in ectopic locations upon overexpression of the neurogenic genes X-ngnr-1 and XNeuroD. Noelin-1 expression in animal caps induces expression of neural markers XBrn-3d and XNeuroD, and co-expression of secreted Noelin-1 with noggin amplifies noggin-induced expression of XBrn-3d and XNeuroD. Furthermore, in animal caps neuralized by expression of noggin, co-expression of Noelin-1 causes expression of neuronal differentiation markers several stages before neurogenesis normally occurs in this tissue. Finally, only secreted forms of the protein can activate sensory marker expression, while all forms of the protein can induce early neurogenesis. This suggests that the cellular localization of Noelin-1 may be important to its function. Thus, Noelin-1 represents a novel secreted factor involved in neurogenesis.", "date": "2001-12-15", "date_type": "published", "publication": "Developmental Biology", "volume": "240", "number": "2", "publisher": "Elsevier", "pagerange": "340-360", "id_number": "CaltechAUTHORS:20160114-101925673", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925673", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "ARCS Foundation" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS41070" } ] }, "doi": "10.1006/dbio.2001.0472", "resource_type": "article", "pub_year": "2001", "author_list": "Moreno, Tanya A. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/xe3x0-30a07", "eprint_id": 64945, "eprint_status": "archive", "datestamp": "2023-08-21 22:45:41", "lastmod": "2023-10-17 22:00:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Id4 expression and its relationship to other Id genes during avian embryonic development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Corneal epithelium; Dorsal myocardium; Embryogenesis; Heart; Id1; Id2; Id3; Id4; Neural crest; Telencephalic vesicles", "note": "\u00a9 2001 Elsevier Science Ltd. \n\nReceived 15 June 2001; received in revised form 11 September 2001; accepted 13 September 2001. Available online 9 October 2001. \n\nWe thank Drs Clare Baker and John Sechrist for critical comments on the manuscript, Dr Laura Gammill for sharing unpublished arrayed library, and other M.B.-F. colleagues for generous help. This work was supported by RO1 HD DE-13223 to M.B.-F. Y.K. is supported by a fellowship from American Heart Association.", "abstract": "We present the sequence and expression pattern of chick Id4 and compare its distribution to that of other vertebrate Id genes. At early stages, Id4 expression is discrete, with transcript transiently expressed in subsets of migrating neural crest cells, the dorsal myocardium, the segmental plate mesoderm, and the tail bud. Later, expression is also observed in the telencephalic vesicles and corneal epithelium. Of all the Id genes, Id4 exhibits the most restricted pattern in the developing nervous system, with little expression in the presumptive neural crest or placodes. Id4 appears in the neural tube much later than other Id genes. However, all four Id genes display overlapping patterns in the branchial arches and tail bud.", "date": "2001-12", "date_type": "published", "publication": "Mechanisms of Development", "volume": "109", "number": "2", "publisher": "Elsevier", "pagerange": "341-345", "id_number": "CaltechAUTHORS:20160302-071130549", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-071130549", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1 HD DE-13223" }, { "agency": "American Heart Association" } ] }, "doi": "10.1016/S0925-4773(01)00576-7", "resource_type": "article", "pub_year": "2001", "author_list": "Kee, Yun and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jhkd9-ksp39", "eprint_id": 64943, "eprint_status": "archive", "datestamp": "2023-08-21 22:45:25", "lastmod": "2023-10-17 22:00:09", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Temporally and spatially restricted expression of the helix\u2013loop\u2013helix transcriptional regulator Id1 during avian embryogenesis", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Branchial arches; Embryogenesis; Epibranchial placodes; Id1; Limb buds; Neural crest; Neural tube; Olfactory placode; Rhombomeres", "note": "\u00a9 2001 Elsevier Science Ltd. \n\nReceived 15 June 2001; received in revised form 11 September 2001; accepted 13 September 2001. Available online 9 October 2001. \n\nWe thank Drs Clare Baker and John Sechrist for critical comments on the manuscript, Drs Jeong Kyo Yoon and Helen McBride for helpful comments on the yeast two-hybrid screening and Johanna Tan and other M.B.-F. colleagues for generous help. This work was supported by RO1 HD DE-13223 to M.B-F. Y.K. is supported by a fellowship from American Heart Association.", "abstract": "We isolated the chick orthologue of the Id1 helix\u2013loop\u2013helix gene and analyzed its expression pattern during early chick embryo development by whole-mount in situ hybridization. The Id1 expression pattern is dynamic and confined to discrete locations including the neural plate border, prospective olfactory placode, hindbrain, mesenchyme of distal branchial arches and adjacent to placodes, and the distal mesoderm of the limb buds.", "date": "2001-12", "date_type": "published", "publication": "Mechanisms of Development", "volume": "109", "number": "2", "publisher": "Elsevier", "pagerange": "331-335", "id_number": "CaltechAUTHORS:20160302-071130023", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-071130023", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1 HD DE-13223" }, { "agency": "American Heart Association" } ] }, "doi": "10.1016/S0925-4773(01)00574-3", "resource_type": "article", "pub_year": "2001", "author_list": "Kee, Yun and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rks88-bpk55", "eprint_id": 64944, "eprint_status": "archive", "datestamp": "2023-08-21 22:45:33", "lastmod": "2023-10-17 22:00:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kee-Yun", "name": { "family": "Kee", "given": "Yun" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The transcriptional regulator Id3 is expressed in cranial sensory placodes during early avian embryonic development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Dermomyotome; Epiphysis; Id3; Foregut endoderm; Lens placode; Nephric primordium; Olfactory placode; Otic placode; Somites; Stomodeum", "note": "\u00a9 2001 Elsevier Science Ltd. \n\nReceived 15 June 2001; received in revised form 11 September 2001; accepted 13 September 2001. Available online 9 October 2001. \n\nWe thank Drs Clare Baker and John Sechrist for critical comments on the manuscript, Dr Laura Gammill for sharing unpublished arrayed library, and Johanna Tan and other MBF colleagues for generous help. This work was supported by RO1 HD DE-13223 to M.B.-F. Y.K. is supported by a fellowship from American Heart Association.", "abstract": "The chick homologue of the helix\u2013loop\u2013helix gene Id3 was isolated, and its expression pattern was analyzed during early stages of chick development. Chick Id3 is dynamically expressed in the olfactory, lens, and otic placodes. It is also observed in the epiphysis, nephric primordium, stomodeum, dermomyotome, distal branchial arches, dorsolateral hindbrain, foregut endoderm, dorsal spinal cord, and somites.", "date": "2001-12", "date_type": "published", "publication": "Mechanisms of Development", "volume": "109", "number": "2", "publisher": "Elsevier", "pagerange": "337-340", "id_number": "CaltechAUTHORS:20160302-071130299", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160302-071130299", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO1 HD DE-13223" }, { "agency": "American Heart Association" } ] }, "doi": "10.1016/S0925-4773(01)00575-5", "resource_type": "article", "pub_year": "2001", "author_list": "Kee, Yun and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tb4xb-wcr23", "eprint_id": 65410, "eprint_status": "archive", "datestamp": "2023-08-19 07:58:31", "lastmod": "2023-10-18 15:59:37", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nellemann-C", "name": { "family": "Nellemann", "given": "Christine" } }, { "id": "De-Bellard-M-E", "name": { "family": "De Bellard", "given": "Maria Elena" } }, { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Laufer-E", "name": { "family": "Laufer", "given": "Ed" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Excess Lunatic Fringe Causes Cranial Neural Crest Over-Proliferation", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2001 Academic Press. \n\nSubmitted for publication January 8, 2001. Revised March 23, 2001. Accepted March 28, 2001. Published online May 30, 2001. \n\nWe thank Dr. Gerry Weinmaster for helpful comments on the manuscript and Ruel Velasco for help with sectioning. This work was supported by NS36585 and DE13223.", "abstract": "Lunatic fringe is a vertebrate homologue of Drosophila fringe, which plays an important role in modulating Notch signaling. This study examines the distribution of chick lunatic fringe at sites of neural crest formation and explores its possible function by ectopic expression. Shortly after neural tube closure, lunatic fringe is expressed in most of the neural tube, with the exception of the dorsal midline containing presumptive neural crest. Thus, there is a fringe/non-fringe border at the site of neural crest production. Expression of excess lunatic fringe in the cranial neural tube and neural crest by retrovirally mediated gene transfer resulted in a significant increase (\u223c60%) in the percentage of cranial neural crest cells 1 day after infection. This effect was mediated by an increase in cell division as assayed by BrdU incorporation. Infected embryos had an up-regulation of Delta-1 in the dorsal neural tube and redistribution of Notch-1 to the lumen of the neural tube, confirming that excess fringe modulates Notch signaling. These findings point to a novel role for lunatic fringe in regulating cell division and/or production of neural crest cells by the neural tube.", "date": "2001-07-01", "date_type": "published", "publication": "Developmental Biology", "volume": "235", "number": "1", "publisher": "Elsevier", "pagerange": "121-130", "id_number": "CaltechAUTHORS:20160317-081242469", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160317-081242469", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "DE13223" } ] }, "doi": "10.1006/dbio.2001.0279", "resource_type": "article", "pub_year": "2001", "author_list": "Nellemann, Christine; De Bellard, Maria Elena; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tgzvv-eff75", "eprint_id": 63680, "eprint_status": "archive", "datestamp": "2023-08-21 22:20:36", "lastmod": "2023-10-17 15:27:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Knecht-A-K", "name": { "family": "Knecht", "given": "Anne K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "DBHR, a Gene with Homology to Dopamine \u03b2-Hydroxylase, Is Expressed in the Neural Crest throughout Early Development", "ispublished": "pub", "full_text_status": "restricted", "keywords": "DBHR; MOX; monooxygenase; dopamine-\u03b2-hydroxylase (DBH); chick development; neural crest; hindbrain", "note": "\u00a9 2001 Academic Press. \n\nReceived for publication December 18, 2000. Revised March 26, 2001. Accepted March 26, 2001. Published online May 11, 2001. \n\nWe thank Dr. W. Funk for communication of the human MOX sequence, Dr. S. Bryant and Drs. A. Nieto and D. Wilkinson for chick embryo libraries, Dr. J. Sechrist for assistance with anatomical identification, and Drs. C. Baker and A. Groves for critical reading of the manuscript. This work was supported by grants from the National Institutes of Health (DE13223 and NS41070) to M.B.F., and A.K.K. was supported by postdoctoral fellowships from the Cancer Research Fund of the Damon Runyon\u2013Walter Winchell Foundation and from the National Institutes of Health (DE14134).", "abstract": "In a screen for genes involved in neural crest development, we identified DBHR (DBH-Related), a putative monooxygenase with low homology to dopamine \u03b2-hydroxylase (DBH). Here, we describe novel expression patterns for DBHR in the developing embryo and particularly the neural crest. DBHR is an early marker for prospective neural crest, with earliest expression at the neural plate border where neural crest is induced. Furthermore, DBHR expression persists in migrating neural crest and in many, though not all, crest derivatives. DBHR is also expressed in the myotome, from the earliest stages of its formation, and in distinct regions of the neural tube, including even-numbered rhombomeres of the hindbrain. In order to investigate the signals that regulate its segmented pattern in the hindbrain, we microsurgically rotated the rostrocaudal positions of rhombomeres 3/4. Despite their ectopic position, both rhombomeres continued to express DBHR at the level appropriate for their original location, indicating that DBHR is regulated autonomously within rhombomeres. We conclude that DBHR is a divergent member of a growing family of DBH-related genes; thus, DBHR represents a completely new type of neural crest marker, expressed throughout the development of the neural crest, with possible functions in cell\u2013cell signaling.", "date": "2001-06-15", "date_type": "published", "publication": "Developmental Biology", "volume": "234", "number": "2", "publisher": "Elsevier", "pagerange": "365-375", "id_number": "CaltechAUTHORS:20160114-101925148", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160114-101925148", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE13223" }, { "agency": "NIH", "grant_number": "NS41070" }, { "agency": "Damon Runyon\u2013Walter Winchell Foundation Cancer Research" }, { "agency": "NIH", "grant_number": "DE14134" } ] }, "doi": "10.1006/dbio.2001.0275", "resource_type": "article", "pub_year": "2001", "author_list": "Knecht, Anne K. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2g821-y9e58", "eprint_id": 65291, "eprint_status": "archive", "datestamp": "2023-08-21 22:08:07", "lastmod": "2023-10-18 14:30:12", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Vertebrate Cranial Placodes I. Embryonic Induction", "ispublished": "pub", "full_text_status": "restricted", "keywords": "placode; olfactory; hyophyseal; adenohypophysis; lens; trigeminal; lateral line; otic; epibranchial; zebrafish; Xenopus; axolotl; chick; mouse", "note": "\u00a9 2001 Academic Press. \n\nReceived for publication September 11, 2000, Accepted 15 December 2000. \n\nWe are grateful to Carole LaBonne, Andy Groves, and the anonymous reviewers for insightful comments on the manuscript and Andrea Streit, Gerhard Schlosser, and Tom Schilling for advice. Thanks to Andy Groves, Philippa Francis-West, Sally Moody, Gerhard Schlosser, and Paul Scotting for communicating results prior to publication.", "abstract": "Cranial placodes are focal regions of thickened ectoderm in the head of vertebrate embryos that give rise to a wide variety of cell types, including elements of the paired sense organs and neurons in cranial sensory ganglia. They are essential for the formation of much of the cranial sensory nervous system. Although relatively neglected today, interest in placodes has recently been reawakened with the isolation of molecular markers for different stages in their development. This has enabled a more finely tuned approach to the understanding of placode induction and development and in some cases has resulted in the isolation of inducing molecules for particular placodes. Both morphological and molecular data support the existence of a preplacodal domain within the cranial neural plate border region. Nonetheless, multiple tissues and molecules (where known) are involved in placode induction, and each individual placode is induced at different times by a different combination of these tissues, consistent with their diverse fates. Spatiotemporal changes in competence are also important in placode induction. Here, we have tried to provide a comprehensive review that synthesises the highlights of a century of classical experimental research, together with more modern evidence for the tissues and molecules involved in the induction of each placode.", "date": "2001-04-01", "date_type": "published", "publication": "Developmental Biology", "volume": "232", "number": "1", "publisher": "Elsevier", "pagerange": "1-61", "id_number": "CaltechAUTHORS:20160311-072900446", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160311-072900446", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1006/dbio.2001.0156", "resource_type": "article", "pub_year": "2001", "author_list": "Baker, Clare V. H. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/d50fw-crc94", "eprint_id": 65620, "eprint_status": "archive", "datestamp": "2023-08-21 21:41:23", "lastmod": "2023-10-18 16:11:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Avian neural crest cell fate decisions: a diffusible signal mediates induction of neural crest by the ectoderm", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Induction; Peripheral nervous system; Transfilter", "note": "\u00a9 2000 ISDN. Published by Elsevier Science Ltd.\n\nThis work was supported by USPHS NS34671 and NS36585 to MBF.", "abstract": "During neurulation, a region of central ectoderm becomes thickened to form the neural plate which then folds upon itself to generate the neural tube, from which all neurons and glia cells of the central nervous system arise. Neural crest cells form at the border of the neural plate, where it abuts the prospective epidermis. The neural crest is a transient population of cells that undergo an epithelial-mesenchymal transition, become highly migratory and subsequently differentiate into most of the peripheral nervous systems as well as numerous other derivatives. The origin of neural crest cells at the epidermal\u2013neural plate border suggests that an interaction between these two tissues may be involved in neural crest formation. By experimentally juxtaposing prospective epidermis with naive neural plate, we previously showed that an inductive interaction between these tissues can generate neural crest cells. Here, we further characterize the nature of this inductive interaction by co-culturing isolated neural plate and prospective epidermis on opposing sides of polycarbonate filters with differing pore sizes. We find that neural crest cells are generated even when epidermis and neural plate are separated by filters that do not allow cell contact. These results suggest that the epidermal inducer is a diffusible, secreted molecule. We discuss the developmental potential of neural crest precursors and lineage decisions that effect their differentiation into numerous derivatives.", "date": "2000-11", "date_type": "published", "publication": "International Journal of Developmental Neuroscience", "volume": "18", "number": "7", "publisher": "Elsevier", "pagerange": "621-627", "id_number": "CaltechAUTHORS:20160323-104946656", "issn": "0736-5748", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-104946656", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" } ] }, "doi": "10.1016/S0736-5748(00)00037-X", "resource_type": "article", "pub_year": "2000", "author_list": "Selleck, Mark A. J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jvc4d-p5m49", "eprint_id": 80708, "eprint_status": "archive", "datestamp": "2023-08-19 06:16:56", "lastmod": "2023-10-23 15:11:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The Neural Crest in Development and Evolution by Brian K. Hall [Book Review]", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2000 Elsevier Science Ltd. \n\nAvailable online 27 November 2000. \n\nBook review of: The Neural Crest in Development and Evolution by Brian K. Hall, Springer, 1999. 313 pages. ISBN: 0 387 98702 9", "abstract": "The neural crest is a fascinating population of cells that is unique to craniates. These cells arise as the nervous system forms. Initially, they are contiguous with the developing CNS (presumptive brain and spinal cord). Shortly after neurulation, however, they migrate away from their site of origin and undergo extensive and stereotypic movements throughout the body. Upon reaching their final destination, they differentiate into a wide array of derivatives, including much of the PNS and craniofacial skeleton, as well as some endocrine and smooth-muscle cells. Their migratory ability, extensive diversity of derivatives and accessibility to manipulation has made this cell population an excellent model system for both developmental and evolutionary studies. Furthermore, they are susceptible to abnormal development, which gives rise to notable birth defects and cancerous derivatives. Thus, the neural crest represents a cell population of great clinical importance.", "date": "2000-09-01", "date_type": "published", "publication": "Trends in Neurosciences", "volume": "23", "number": "9", "publisher": "Elsevier", "pagerange": "445-446", "id_number": "CaltechAUTHORS:20170822-155618370", "issn": "0166-2236", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20170822-155618370", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0166-2236(00)01567-8", "resource_type": "article", "pub_year": "2000", "author_list": "Baker, Clare and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mcf3c-rxt03", "eprint_id": 12782, "eprint_status": "archive", "datestamp": "2023-08-19 06:11:23", "lastmod": "2023-10-17 20:55:17", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Groves-A-K", "name": { "family": "Groves", "given": "Andrew K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Competence, specification and commitment in otic placode induction", "ispublished": "pub", "full_text_status": "public", "keywords": "Otic Placode, Induction, Chicken, Competence, Specification, Commitment", "note": "Copyright \u00a9 2000 by Company of Biologists. \n\nAccepted 4 June 2000; published on WWW 20 July 2000. \n\nWe thank Julian Lewis, David Ish-Horowicz, Domingos Henrique, Brian Huston and Hisato Kondoh for cDNA probes. A.K.G. wishes to thank Clare Baker for her support and advice, and for her comments on the manuscript, Heather Etchevers for advice on the paraffin in situ protocol and Butch Welch for assistance with color image processing. A.K.G. is supported by the House Ear Institute and an NIH Small Project Grant, DC03630-01. This work was also supported by NIH grants NS 13815 and NS 36585 to M.B.-F.\n\nPublished - GROdev00.pdf
", "abstract": "The inner ear is induced from cranial ectoderm adjacent to the hindbrain. Despite almost a century of study, the molecular mechanisms of inner ear induction remain obscure. We have identified four genes expressed very early in the anlage of the inner ear, the otic placode. Pax-2, Sox-3, BMP-7 and Notch are all expressed in placodal ectoderm from the 4-5 somite stage (ss) onwards, well before the otic placode becomes morphologically visible at the 12-14ss. We have used these four molecular markers to show that cranial ectoderm becomes specified to form the otic placode at the 4-6ss, and that this ectoderm is committed to a placodal fate by the 10ss. We also demonstrate that much of the embryonic ectoderm is competent to generate an otic placode if taken at a sufficiently early age. We have mapped the location of otic placode-inducing activity along the rostrocaudal axis of the embryo, and have determined that this activity persists at least until the 10ss. Use of the four molecular otic placode markers suggests that induction of the otic placode in birds occurs earlier than previously thought, and proceeds in a series of steps that are independently regulated.", "date": "2000-08-15", "date_type": "published", "publication": "Development", "volume": "127", "number": "16", "publisher": "Company of Biologists", "pagerange": "3489-3499", "id_number": "CaltechAUTHORS:GROdev00", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:GROdev00", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "House Ear Institute" }, { "agency": "NIH", "grant_number": "DC03630-01" }, { "agency": "NIH", "grant_number": "NS 13815" }, { "agency": "NIH", "grant_number": "NS 36585" } ] }, "primary_object": { "basename": "GROdev00.pdf", "url": "https://authors.library.caltech.edu/records/mcf3c-rxt03/files/GROdev00.pdf" }, "resource_type": "article", "pub_year": "2000", "author_list": "Groves, Andrew K. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t6zfb-bjr06", "eprint_id": 63674, "eprint_status": "archive", "datestamp": "2023-08-21 21:30:12", "lastmod": "2023-10-17 15:27:19", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Linker-C", "name": { "family": "Linker", "given": "Claudia" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Mayor-R", "name": { "family": "Mayor", "given": "Roberto" } } ] }, "title": "Relationship between Gene Expression Domains of Xsnail, Xslug, and Xtwist and Cell Movement in the Prospective Neural Crest of Xenopus", "ispublished": "pub", "full_text_status": "restricted", "keywords": "gastrulation; neurulation; DiI; neural crest", "note": "\u00a9 2000 Academic Press.\n\n Received 8 December 1999, Revised 28 March 2000, Accepted 28 March 2000, Available online 25 March 2002. \n\nWe thank Drs. P. Kulesa, G. Belford, A. Collazo, S. Ruffin, and S. Fraser for their kind help with image processing and analysis. We are very grateful to Dr. F. Bru\u00a8 cher for his computational advice. We thank Dr. S. He-Kee for her extensive help with videomicroscopy. We thank Dr. N. Papalopulu for her helpful recommendations for the in situ hybridization in sections, Dr. J. Gurdon for providing us\nwith Xtwist probe, and Dr. K. Dale for her comments on the manuscript. Special thanks to the members of the Bronner-Fraser laboratory for their kindness and in particular to Ella Moreno for embryological expertise. This investigation was supported by grants from Fondecyt 1990570, by the Universidad de Chile, by NSF Grant INT-9722460, and by USPHS NS36585; C.L. was funded by Fundacion Andes.", "abstract": "The markers Xslug, Xsnail, and Xtwist all are expressed in the presumptive neural folds and are thought to delineate the presumptive neural crest. However, their interrelationship and relative spatiotemporal distributions are not well understood. Here, we present a detailed in situ hybridization analysis of the relative patterns of expression of these transcription factors from gastrulation through neurulation and post-neural crest migration. The three genes mark the prospective neural crest and roof plate, coming on sequentially, with Xsnail preceding Xslug preceding Xtwist. By combining gene expression analysis with a fate map of the same region using DiI labeling, we determined the correspondence between early and late domains of gene expression. At the beginning of gastrulation, Xsnail is present in a unique domain of expression in a lateral region of the embryo in both superficial and deep layers of the ectoderm, as are Xslug and Xtwist. During gastrulation and neurulation, the superficial layer moves faster toward the dorsal midline than the deep layer, producing a relative shift in these cell populations. By early neurula stage, the Xsnail domain is split into a medial domain in the superficial ectoderm (fated to become the roof plate) and a lateral domain in the deep layer of the ectoderm (fated to become neural crest). Xsnail is down-regulated in the most anterior neural plate and up-regulated in the posterior neural plate. Our results show that changes in the expression of Xsnail, Xslug, and Xtwist are a consequence of active cell movement in some regions coupled with dynamic changes in gene expression in other regions.", "date": "2000-08-15", "date_type": "published", "publication": "Developmental Biology", "volume": "224", "number": "2", "publisher": "Elsevier", "pagerange": "215-225", "id_number": "CaltechAUTHORS:20160114-082655382", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160114-082655382", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico (FONDECYT)", "grant_number": "1990570" }, { "agency": "NSF", "grant_number": "INT-9722460" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "Universidad de Chile" }, { "agency": "Fundacion Andes" } ] }, "doi": "10.1006/dbio.2000.9723", "resource_type": "article", "pub_year": "2000", "author_list": "Linker, Claudia; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g7kw5-1pf16", "eprint_id": 65287, "eprint_status": "archive", "datestamp": "2023-08-19 06:10:18", "lastmod": "2023-10-18 14:29:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "orcid": "0000-0002-7699-0173" } ] }, "title": "Pattern formation and developmental mechanisms: The cell biological basis of inductive signaling", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2000 Elsevier Science Ltd. \n\nAvailable online 14 July 2000.", "abstract": "A central issue in developmental biology is how a complex organism arises from a single cell. In both plant and animal development, this occurs by a combination of intrinsic information within cells as well as signals transmitted between cells that result in proper patterning of the embryo. Because of the central role of cell communication, intercellular signaling and signal transduction is an important consideration for understanding development as well as the interplay of signaling and transcription. The transcriptional state of the cell depends upon its prior history and the signals it receives. In turn, the signals produced by a cell and its ability to respond to signals are programmed to a large extent by its transcriptional state. The particular wiring of these signals\u2192transcription\u2192signals circuits determines how cells communicate to specify their fates in particular patterns.", "date": "2000-08-01", "date_type": "published", "publication": "Current Opinion in Genetics and Development", "volume": "10", "number": "4", "publisher": "Current Biology Ltd", "pagerange": "347-349", "id_number": "CaltechAUTHORS:20160311-065659955", "issn": "0959-437X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160311-065659955", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0959-437X(00)00094-0", "resource_type": "article", "pub_year": "2000", "author_list": "Bronner-Fraser, Marianne and Sternberg, Paul W." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dwbrs-k9617", "eprint_id": 11891, "eprint_status": "archive", "datestamp": "2023-08-21 21:23:22", "lastmod": "2023-10-17 15:57:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Establishing neuronal identity in vertebrate neurogenic placodes", "ispublished": "pub", "full_text_status": "public", "keywords": "Placodes, Trigeminal placode, nodose placode, Pax2, Pax3, Phox2a, Chick, Quail, Sensory neurons", "note": "Copyright \u00a9 2000 by Company of Biologists. \n\nAccepted 28 April; published on WWW 22 June 2000. \n\nThanks to Domingos Henrique and Jean-Fran\u00e7ois Brunet for the Pax2 and Phox2a in situ probes respectively. We are very grateful to Andy Groves and Helen McBride for discussion and helpful comments on the manuscript. This work was supported by NS-36585 and NS-13815 (M.B.-F.) and by American Heart Association Fellowship 1171-F11 (C.V.H.B.).\n\nPublished - BAKdev00.pdf
", "abstract": "The trigeminal and epibranchial placodes of vertebrate embryos form different types of sensory neurons. The trigeminal placodes form cutaneous sensory neurons that innervate the face and jaws, while the epibranchial placodes (geniculate, petrosal and nodose) form visceral sensory neurons that innervate taste buds and visceral organs. In the chick embryo, the ophthalmic trigeminal (opV) placode expresses the paired homeodomain transcription factor Pax3 from very early stages, while the epibranchial placodes express Pax2. Here, we show that Pax3 expression in explanted opV placode ectoderm correlates at the single cell level with neuronal specification and with commitment to an opV fate. When opV (trigeminal) ectoderm is grafted in place of the nodose (epibranchial) placode, Pax3-expressing cells form Pax3-positive neurons on the same schedule as in the opV placode. In contrast, Pax3-negative cells in the grafted ectoderm are induced to express the epibranchial placode marker Pax2 and form neurons in the nodose ganglion that express the epibranchial neuron marker Phox2a on the same schedule as host nodose neurons. They also project neurites along central and peripheral nodose neurite pathways and survive until well after the main period of cell death in the nodose ganglion. The older the opV ectoderm is at the time of grafting, the more Pax3-positive cells it contains and the more committed it is to an opV fate. Our results suggest that, within the neurogenic placodes, there does not appear to be a two-step induction of 'generic' neurons followed by specification of the neuron to a particular fate. Instead, there seems to be a one-step induction in which neuronal subtype identity is coupled to neuronal differentiation.", "date": "2000-07-15", "date_type": "published", "publication": "Development", "volume": "127", "number": "14", "publisher": "Company of Biologists", "pagerange": "3045-3056", "id_number": "CaltechAUTHORS:BAKdev00", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BAKdev00", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS13815" }, { "agency": "American Heart Association", "grant_number": "1171-F11" } ] }, "primary_object": { "basename": "BAKdev00.pdf", "url": "https://authors.library.caltech.edu/records/dwbrs-k9617/files/BAKdev00.pdf" }, "resource_type": "article", "pub_year": "2000", "author_list": "Baker, Clare V. H. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2k09s-3z568", "eprint_id": 12901, "eprint_status": "archive", "datestamp": "2023-08-19 06:02:35", "lastmod": "2023-10-17 21:13:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kulesa-P-M", "name": { "family": "Kulesa", "given": "Paul" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott" }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest, Hindbrain, Cell migration, In ovo, Ablation, Repatterning, Time-lapse imaging, Chick", "note": "Copyright \u00a9 2000 by Company of Biologists. \n\nAccepted 4 April; published on WWW 13 June 2000. \n\nThis work was supported by USPHS HD15527 and a grant from the Muscular Dystrophy Foundation to M.B.F., and a Burroughs-Wellcome PPG (AR42671) to S.F. P.K. would like to thank the Sloan Foundation and the Burroughs-Wellcome Computational Molecular Biology Initiative at Caltech for their generous support. We are grateful to H. McBride and D. Crotty for critical reading of the manuscript.\n\nPublished - KULdev00b.pdf
", "abstract": "Previous analyses of single neural crest cell trajectories\nhave suggested important roles for interactions between\nneural crest cells and the environment, and amongst neural\ncrest cells. To test the relative contribution of intrinsic\nversus extrinsic information in guiding cells to their\nappropriate sites, we ablated subpopulations of\npremigratory chick hindbrain neural crest and followed\nthe remaining neural crest cells over time using a new in\novo imaging technique. Neural crest cell migratory\nbehaviors are dramatically different in ablated compared\nwith unoperated embryos. Deviations from normal\nmigration appear either shortly after cells emerge from the\nneural tube or en route to the branchial arches, areas where\ncell-cell interactions typically occur between neural crest\ncells in normal embryos. Unlike the persistent, directed\ntrajectories in normal embryos, neural crest cells\nfrequently change direction and move somewhat\nchaotically after ablation. In addition, the migration of\nneural crest cells in collective chains, commonly observed\nin normal embryos, was severely disrupted. Hindbrain\nneural crest cells have the capacity to reroute their\nmigratory pathways and thus compensate for missing\nneural crest cells after ablation of neighboring populations.\nBecause the alterations in neural crest cell migration are\nmost dramatic in regions that would normally foster cell-cell\ninteractions, the trajectories reported here argue that\ncell-cell interactions have a key role in the shaping of the\nneural crest migration.", "date": "2000-07-01", "date_type": "published", "publication": "Development", "volume": "127", "number": "13", "publisher": "Company of Biologists", "pagerange": "2843-2852", "id_number": "CaltechAUTHORS:KULdev00b", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:KULdev00b", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD15527" }, { "agency": "Muscular Dystrophy Association of America" }, { "agency": "Burroughs-Wellcome", "grant_number": "AR42671" }, { "agency": "Alfred P. Sloan Foundation" }, { "agency": "Burroughs-Wellcome Computational Molecular Biology Initiative, Caltech" } ] }, "primary_object": { "basename": "KULdev00b.pdf", "url": "https://authors.library.caltech.edu/records/2k09s-3z568/files/KULdev00b.pdf" }, "resource_type": "article", "pub_year": "2000", "author_list": "Kulesa, Paul; Fraser, Scott; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cwynj-b7b83", "eprint_id": 28381, "eprint_status": "archive", "datestamp": "2023-08-19 05:59:33", "lastmod": "2023-10-24 17:51:13", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perissinotto-Daniela", "name": { "family": "Perissinotto", "given": "Daniela" } }, { "id": "Iacopetti-Paola", "name": { "family": "Iacopetti", "given": "Paola" } }, { "id": "Bellina-Isabella", "name": { "family": "Bellina", "given": "Isabella" } }, { "id": "Doliana-Roberto", "name": { "family": "Doliana", "given": "Roberto" }, "orcid": "0000-0002-8324-7564" }, { "id": "Colombatti-Alfonso", "name": { "family": "Colombatti", "given": "Alfonso" }, "orcid": "0000-0002-3676-2379" }, { "id": "Pettway-Unno-Zo\u00e9", "name": { "family": "Pettway", "given": "Zo\u00e9" }, "orcid": "0000-0002-5176-185X" }, { "id": "Shinomura-Tamayuki", "name": { "family": "Shinomura", "given": "Tamayuki" } }, { "id": "Kimata-Koji", "name": { "family": "Kimata", "given": "Koji" } }, { "id": "M\u00f6rgelin-Matthias", "name": { "family": "M\u00f6rgelin", "given": "Matthias" }, "orcid": "0000-0002-6212-6990" }, { "id": "L\u00f6fberg-Jan", "name": { "family": "L\u00f6fberg", "given": "Jan" } }, { "id": "Perris-Roberto", "name": { "family": "Perris", "given": "Roberto" }, "orcid": "0000-0001-5626-5233" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan", "ispublished": "pub", "full_text_status": "public", "keywords": "PG-M/versican, Neural crest, Cell migration, Aggrecan, Proteoglycan, Chick", "note": "\u00a9 2000 The Company of Biologists. \n\nAccepted 11 April; published on WWW 13 June 2000. \n\nWe thank Elena Gabriele, Michela Zanbon and Maria Teresa Mucignat for their technical assistance, Paola Spessotto for her\nassistance with the confocal laser microscopy, Gianluca Tell for performing HPLC chromatographies, and Guido David, Dick Heineg\u00e5rd, Firoz Rahemtulla and Michael Sorrell for their proteoglycan and antibody contribution. The work was supported by grants from Associazione Italiana della Ricerca sul Cancro (AIRC) and Fondo Sanitario Nazionale (FSN, RF-95 and RF-96).\n\nPublished - PERdev00.pdf
", "abstract": "It has been proposed that hyaluronan-binding proteoglycans play an important role as guiding cues during neural crest (NC) cell migration, but their precise function has not been elucidated. In this study, we examine the distribution, structure and putative role of the two major hyaluronan-binding proteoglycans, PG-M/versicans and aggrecan, during the course of avian NC development. PG-M/versicans V0 and V1 are shown to be the prevalent isoforms at initial and advanced phases of NC cell movement, whereas the V2 and V3 transcripts are first detected following gangliogenesis. During NC cell dispersion, mRNAs for PG-M/versicans V0/V1 are transcribed by tissues lining the NC migratory pathways, as well as by tissues delimiting nonpermissive areas. Immunohistochemistry confirm the deposition of the macromolecules in these regions and highlight regional differences in the density of these proteoglycans. PG-M/versicans assembled within the sclerotome rearrange from an initially uniform distribution to a preferentially caudal localization, both at the mRNA and protein level. This reorganization is a direct consequence of the metameric NC cell migration through the rostral portion of the somites. As suggested by previous in situ hybridizations, aggrecan shows a virtually opposite distribution to PG-M/versicans being confined to the perinotochordal ECM and extending dorsolaterally in a segmentally organized manner eventually to the entire spinal cord at axial levels interspacing the ganglia. PG-M/versicans purified from the NC migratory routes are highly polydispersed, have an apparent M(r) of 1,200-2,000 kDa, are primarily substituted with chondroitin-6-sulfates and, upon chondroitinase ABC digestion, are found to be composed of core proteins with apparent M(r)of 360\u2013530, 000. TEM/rotary shadowing analysis of the isolated PG-M/versicans confirmed that they exhibit the characteristic bi-globular shape, have core proteins with sizes predicted for the V0/V1 isoforms and carry relatively few extended glycosaminoglycan chains. Orthotopical implantation of PG-M/versicans immobilized onto transplantable micromembranes tend to 'attract' moving cells toward them, whereas similar implantations of a notochordal type-aggrecan retain both single and cohorts of moving NC cells in close proximity of the implant and thereby perturb their spatiotemporal migratory pattern. NC cells fail to migrate through three-dimensional collagen type I-aggrecan substrata in vitro, but locomote in a haptotactic manner through collagen type I-PG-M/versican V0 substrata via engagement of HNK-1 antigen-bearing cell surface components. The present data suggest that PG-M/versicans and notochordal aggrecan exert divergent guiding functions during NC cell dispersion, which are mediated by both their core proteins and glycosaminoglycan side chains and may involve 'haptotactic-like' motility phenomena. Whereas aggrecan defines strictly impenetrable embryonic areas, PG-M/versicans are central components of the NC migratory pathways favoring the directed movement of the cells.", "date": "2000-07", "date_type": "published", "publication": "Development", "volume": "127", "number": "13", "publisher": "Company of Biologists", "pagerange": "2823-2842", "id_number": "CaltechAUTHORS:20111209-073948808", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20111209-073948808", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Associazione Italiana della Ricerca sul Cancro (AIRC)" }, { "agency": "Fondo Sanitario Nazionale (FSN)", "grant_number": "RF-95" }, { "agency": "Fondo Sanitario Nazionale (FSN)", "grant_number": "RF-96" } ] }, "primary_object": { "basename": "PERdev00.pdf", "url": "https://authors.library.caltech.edu/records/cwynj-b7b83/files/PERdev00.pdf" }, "resource_type": "article", "pub_year": "2000", "author_list": "Perissinotto, Daniela; Iacopetti, Paola; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dr53y-43v88", "eprint_id": 63300, "eprint_status": "archive", "datestamp": "2023-08-21 21:20:09", "lastmod": "2023-10-25 23:42:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Basch-M-L", "name": { "family": "Basch", "given": "Mart\u00edn L." } }, { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Timing and Competence of Neural Crest Formation", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest cells; ectoderm; Slug; neural plate; BMP-4", "note": "\u00a9 2000 S. Karger AG, Basel.", "abstract": "Neural crest cells can be induced by an interaction between neural plate and ectoderm. To clarify the timing and nature of these inductive interactions, we have examined the time of competence of the neural plate to become neural crest as well as the time of neural fold specification. The neural plate is competent to respond to inductive interactions with the nonneural ectoderm for a limited period, rapidly losing its responsive ability after stage 10. In contrast, nonneural ectoderm from numerous stages retains the ability to induce neural crest cells from competent neural plate. When neural folds are explanted to test their ability to produce neural crest without further tissue interactions, we find that folds derived from all rostrocaudal levels of the open neural plate are already specified to express the neural crest marker Slug. However, additional signals may be required for maintenance of Slug expression, since the transcript is later down-regulated in vitro in the absence of tissue interactions. Taken together, these results suggest that there are multiple stages of neural crest induction. The earliest induction must have occurred by the end of gastrulation, since the newly formed neural fold population is already specified to form neural crest. However, isolated neural folds eventually down-regulate Slug, suggesting a second phase that maintains neural crest formation. Thus, induction of the neural crest may involve multiple and sustained tissue interactions.", "date": "2000-07", "date_type": "published", "publication": "Developmental Neuroscience", "volume": "22", "number": "3", "publisher": "Karger", "pagerange": "217-227", "id_number": "CaltechAUTHORS:20160101-192046954", "issn": "0378-5866", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-192046954", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1159/000017444", "resource_type": "article", "pub_year": "2000", "author_list": "Basch, Mart\u00edn L.; Selleck, Mark A. J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/y3mc8-sgc64", "eprint_id": 12094, "eprint_status": "archive", "datestamp": "2023-08-21 21:17:50", "lastmod": "2023-10-17 16:31:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Epperlein-H-H", "name": { "family": "Epperlein", "given": "Hans-Henning" } }, { "id": "Meulemans-D", "name": { "family": "Meulemans", "given": "Daniel" } }, { "id": "Steinbeisser-H", "name": { "family": "Steinbeisser", "given": "Herbert" } }, { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Analysis of cranial neural crest migratory pathways in axolotl using cell markers and transplantation", "ispublished": "pub", "full_text_status": "public", "keywords": "DiI, AP-2, Cell movement, Cartilage differentiation, Branchial arches, Axolotl, Neural crest", "note": "Copyright \u00a9 2000 by Company of Biologists. \n\nAccepted 3 April 2000; published on WWW 23 May 2000. \n\nThis work was supported by a Howard Hughes Medical Institute Research Resources Grant and a James H. Zumberge Research and Innovation Fund (M.A.J.S.), by NS36585 and NS34671 to M.B.F. and the Deutsche Forschungsgemeinschaft (Ep8/7-1) to H.-H.E. The 12/101 monoclonal antibody, developed by Jeremy Brockes, was obtained from the Developmental Studies Hybridoma Bank maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, USA.\n\nPublished - EPPdev00.pdf
", "abstract": "We have examined the ability of normal and heterotopically transplanted neural crest cells to migrate along cranial neural crest pathways in the axolotl using focal DiI injections and in situ hybridization with the neural crest marker, AP-2. DiI labeling demonstrates that cranial neural crest cells migrate as distinct streams along prescribed pathways to populate the maxillary and mandibular processes of the first branchial arch, the hyoid arch and gill arches 1-4, following migratory pathways similar to those observed in other vertebrates. Another neural crest marker, the transcription factor AP-2, is expressed by premigratory neural crest cells within the neural folds and migrating neural crest cells en route to and within the branchial arches. Rotations of the cranial neural folds suggest that premigratory neural crest cells are not committed to a specific branchial arch fate, but can compensate when displaced short distances from their targets by migrating to a new target arch. In contrast, when cells are displaced far from their original location, they appear unable to respond appropriately to their new milieu such that they fail to migrate or appear to migrate randomly. When trunk neural folds are grafted heterotopically into the head, trunk neural crest cells migrate in a highly disorganized fashion and fail to follow normal cranial neural crest pathways. Importantly, we find incorporation of some trunk cells into branchial arch cartilage despite the random nature of their migration. This is the first demonstration that trunk neural crest cells can form cartilage when transplanted to the head. Our results indicate that, although cranial and trunk neural crest cells have inherent differences in ability to recognize migratory pathways, trunk neural crest can differentiate into cranial cartilage when given proper instructive cues.", "date": "2000-06-15", "date_type": "published", "publication": "Development", "volume": "127", "number": "12", "publisher": "Company of Biologists", "pagerange": "2751-2761", "id_number": "CaltechAUTHORS:EPPdev00", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:EPPdev00", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Howard Hughes Medical Institute (HHMI)" }, { "agency": "James H. Zumberge Research and Innovation Fund" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)", "grant_number": "Ep8/7-1" } ] }, "primary_object": { "basename": "EPPdev00.pdf", "url": "https://authors.library.caltech.edu/records/y3mc8-sgc64/files/EPPdev00.pdf" }, "resource_type": "article", "pub_year": "2000", "author_list": "Epperlein, Hans-Henning; Meulemans, Daniel; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qrnkn-b1043", "eprint_id": 51691, "eprint_status": "archive", "datestamp": "2023-08-19 05:46:24", "lastmod": "2023-10-18 17:02:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Martin I." } }, { "id": "Vielmetter-E", "name": { "family": "Vielmetter", "given": "Eva" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "N-Cadherin, a Cell Adhesion Molecule Involved in Establishment of Embryonic Left-Right Asymmetry", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2000 American Association for the Advancement of Science. \n\n11 November 1999; accepted 5 April 2000. \n\nSupported by U.S. Public Health Service grant HD 15527 (M.B.-F.), American Heart Association 1140-FI1 (M.I.G-C.), and the Summer Undergraduate Research Fellowship, Caltech (E.V.). We thank C. Baker, A. Groves, C. LaBonne, and B. E. Murray for helpful discussions and comments and R. Velasco for help with sectioning.", "abstract": "Within the bilaterally symmetric vertebrate body plan, many organs develop asymmetrically. Here, it is demonstrated that a cell adhesion molecule, N-cadherin, is one of the earliest proteins to be asymmetrically expressed in the chicken embryo and that its activity is required during gastrulation for proper establishment of the left-right axis. Blocking N-cadherin function randomizes heart looping and alters the expression of Snail and Pitx2, later components of the molecular cascade that regulate left-right asymmetry. However, the expression of other components of this cascade (Nodal and Lefty) was unchanged after blocking N-cadherin function, suggesting the existence of parallel pathways in the establishment of left-right morphogenesis. Here, the results suggest that N-cadherin-mediated cell adhesion events are required for establishment of left-right asymmetry.", "date": "2000-05-12", "date_type": "published", "publication": "Science", "volume": "288", "number": "5468", "publisher": "American Association for the Advancement of Science", "pagerange": "1047-1051", "id_number": "CaltechAUTHORS:20141113-071030883", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141113-071030883", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "American Heart Association", "grant_number": "1140-FI1" }, { "agency": "Caltech Summer Undergraduate Research Fellowship (SURF)" } ] }, "doi": "10.1126/science.288.5468.1047", "resource_type": "article", "pub_year": "2000", "author_list": "Garc\u00eda-Castro, Martin I.; Vielmetter, Eva; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/50rk4-fvm49", "eprint_id": 65262, "eprint_status": "archive", "datestamp": "2023-08-21 21:09:46", "lastmod": "2023-10-18 14:28:15", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "C." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Snail-Related Transcriptional Repressors Are Required in Xenopus for both the Induction of the Neural Crest and Its Subsequent Migration", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest; slug; snail; Xenopus", "note": "\u00a9 2000 Academic Press. \n\nReceived 22 October 1999, Revised 20 December 1999, Accepted 20 December 1999. \n\nWe thank Clare Baker, Ann Knecht, and David McCauley for critical reading of the manuscript and Adrian Gross, Laura Gammill, and members of the laboratory for helpful discussions. pCS21 derivatives were kindly provided by Robert Davis and Dave Turner. C.L. is a fellow of the American Cancer Society. This work was also supported by USPHS NS36585 and NS34671 to M.B-F.", "abstract": "The neural crest is a transient population of precursor cells that arises at the border between the neural plate and prospective epidermis in vertebrate embryos. The earliest known response to neural-crest-inducing signals is the expression of the zinc-finger transcription factors slug and snail. Although it is widely believed that these transcription factors play an essential role in neural crest development, relatively little is understood about their mechanism of action during this process. We have previously shown that overexpression of XSlug leads to expanded expression of neural crest markers and an excess of at least one neural crest derivative, melanocytes. In order to further investigate XSlug function, we overexpressed mutant constructs in which the DNA-binding domain was fused to either the activation domain from Gal4 or the repressor domain from Drosophila Engrailed. The Engrailed repressor fusion was found to mimic the effects of wild-type XSlug, indicating that XSlug functions as a transcriptional repressor during neural crest formation. In contrast, overexpression of either the activation domain fusion or the DNA-binding domain alone was found to inhibit XSlug function. Using a hormone-inducible inhibitory mutant, we show that inhibition of XSlug function at early stages prevents the formation of neural crest precursors, while inhibition at later stages interferes with neural crest migration, demonstrating for the first time that this transcriptional repressor is required during multiple stages of neural crest development.", "date": "2000-05", "date_type": "published", "publication": "Developmental Biology", "volume": "221", "number": "1", "publisher": "Elsevier", "pagerange": "195-205", "id_number": "CaltechAUTHORS:20160310-123654012", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160310-123654012", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Cancer Society" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS34671" } ] }, "doi": "10.1006/dbio.2000.9609", "resource_type": "article", "pub_year": "2000", "author_list": "LaBonne, C. and Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pjcg2-fm016", "eprint_id": 57515, "eprint_status": "archive", "datestamp": "2023-08-19 05:34:58", "lastmod": "2023-10-23 17:29:32", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Barembaum-M", "name": { "family": "Barembaum", "given": "Meyer" } }, { "id": "Moreno-T-A", "name": { "family": "Moreno", "given": "Tanya A." } }, { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "Carole" } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Noelin-1 is a secreted glycoprotein involved in generation of the neural crest", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 2000 Macmillan Publishers Limited.\n\nReceived 4 January 2000; accepted 28 February 2000; published 8 March 2000.\n\nWe thank C. Baker, S. Fraser, M. Dickinson, B. Murray and M. Selleck for helpful discussions and comments on the manuscript and J. Neri and R. Velasco for help with sectioning. This work was supported by NS 36585 and NS 34671. Correspondence and requests for materials should be addressed to M.B-F. Sequences for Noelin-1 and Noelin-2 have been deposited in GenBank (accession nos AF182815 and AF239804, respectively).", "abstract": "The vertebrate neural crest arises at the border of the neural plate during early stages of nervous system development; however, little is known about the molecular mechanisms underlying neural crest formation. Here we identify a secreted protein, Noelin-1, which has the ability to prolong neural crest production. Noelin-1 messenger RNA is expressed in a graded pattern in the closing neural tube. It subsequently becomes restricted to the dorsal neural folds and migrating neural crest. Over expression of Noelin-1 using recombinant retroviruses causes an excess of neural crest emigration and extends the time that the neural tube is competent to generate as well as regenerate neural crest cells. These results support an important role for Noelin-1 in regulating the production of neural crest cells by the neural tube.", "date": "2000-04", "date_type": "published", "publication": "Nature Cell Biology", "volume": "2", "number": "4", "publisher": "Nature Publishing Group", "pagerange": "219-225", "id_number": "CaltechAUTHORS:20150513-161849302", "issn": "1465-7392", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20150513-161849302", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS 36585" }, { "agency": "NIH", "grant_number": "NS 34671" } ] }, "doi": "10.1038/35008643", "resource_type": "article", "pub_year": "2000", "author_list": "Barembaum, Meyer; Moreno, Tanya A.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/72b9m-dwk47", "eprint_id": 63565, "eprint_status": "archive", "datestamp": "2023-08-21 20:53:55", "lastmod": "2023-10-17 15:19:47", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ruffins-S-W", "name": { "family": "Ruffins", "given": "Seth" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A Critical Period for Conversion of Ectodermal Cells to a Neural Crest Fate", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest cells; notochord; Slug; neural plate", "note": "\u00a9 2000 Academic Press. \n\nReceived for publication September 17, 1999; Revised October 28, 1999; Accepted October 28, 1999. \n\nThis work was supported by NIH Grants USPHS NS34671 and NS36585 to M.B.F. and NRSA NS11008-1 to S.R.", "abstract": "Previously, we found that interactions between neural and nonneural ectoderm can generate neural crest cells, with both the ectodermal and the neuroepithelial cells contributing to induced population (M. A. J. Selleck and M. Bronner-Fraser, 1995, Development 121, 525\u2013538). To further characterize the ability of ectodermal cells to form neural crest, we have challenged their normal fate by transplanting them into the neural tube. To ensure that the ectoderm was from nonneural regions, we utilized extraembryonic ectoderm (the proamnion) and transplanted it into the presumptive midbrain of 1.5-day-old chick embryos. We observed that the grafted ectoderm has the capacity to adopt a neural crest fate, responding within a few hours of surgery by turning on neural crest markers HNK-1 and Slug. However, the competence of the ectoderm to respond to neural crest-inducing signals is time limited, declining rapidly in donors older than the 10-somite stage. Similarly, the inductive capacity of the host midbrain declines in a time-dependent fashion. Our results show that extraembryonic ectoderm has the capacity to form neural crest cells given proper inducing signals, expressing both morphological and molecular markers characteristic of neural crest cells.", "date": "2000-02-01", "date_type": "published", "publication": "Developmental Biology", "volume": "218", "number": "1", "publisher": "Elsevier", "pagerange": "13-20", "id_number": "CaltechAUTHORS:20160112-081714331", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160112-081714331", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS34671" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "National Research Service Award (NRSA)", "grant_number": "NS11008-1" } ] }, "doi": "10.1006/dbio.1999.9555", "resource_type": "article", "pub_year": "2000", "author_list": "Ruffins, Seth and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pnww2-c1j12", "eprint_id": 66053, "eprint_status": "archive", "datestamp": "2023-08-22 14:12:33", "lastmod": "2023-10-18 17:05:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Mart\u00edn" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Induction and differentiation of the neural crest", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Induction; Differentiation; Neural crest; Embryos; BMP; TGF-\u03b2", "note": "\u00a9 1999 Elsevier Science Ltd. Available online 28 January 2000. \n\nWe thank Clare Baker for helpful comments on the manuscript. Mart\u00edn Garc\u00eda-Castro was supported by the American Heart Association (No.1140 F11)", "abstract": "The neural crest is a population of cells that forms at the junction between the epidermis and neural plate in vertebrate embryos. Recent progress has elucidated the identity and timing of molecular events responsible for the earliest steps in neural crest development, particularly those involving the induction and its migration. Concomitantly, advances have been made in the identification, purification and generation of neural crest stem cells at various developmental stages that deepens our understanding of the plasticity and restriction of neural crest differentiation.", "date": "1999-12-01", "date_type": "published", "publication": "Current Opinion in Cell Biology", "volume": "11", "number": "6", "publisher": "Elsevier", "pagerange": "695-698", "id_number": "CaltechAUTHORS:20160411-131015683", "issn": "0955-0674", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160411-131015683", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Heart Association", "grant_number": "1140 F11" } ] }, "doi": "10.1016/S0955-0674(99)00038-1", "resource_type": "article", "pub_year": "1999", "author_list": "Garc\u00eda-Castro, Mart\u00edn and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8yyra-rdg62", "eprint_id": 65634, "eprint_status": "archive", "datestamp": "2023-08-19 04:53:11", "lastmod": "2023-10-18 16:12:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ahlgren-S-C", "name": { "family": "Ahlgren", "given": "Sara C." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Inhibition of Sonic hedgehog signaling in vivo results in craniofacial neural crest cell death", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1999 Elsevier Science Ltd. Received: 8 February 1999. Revised: 1 September 1999. Accepted: 4 October 1999.\nPublished: 3 November 1999. Available online 15 January 2001.\n\nThe authors wish to thank Clare Baker and Andy Groves for critical readings of this manuscript, and Christophe Marcelle for helpful discussions during the course of the experiments. Cliff Tabin kindly supplied the chicken Ptc\nand Shh probes. S.C.A. was supported by NIH award # NS07251 and the American Heart Association award # 1168-F1. This work was supported by USPHS NS34671 and NS36585.\n\nSupplemental Material - mmc1.pdf
", "abstract": "Background: Sonic hedgehog (Shh) is well known for its role in patterning tissues, including structures of the head. Haploinsufficiency for SHH in humans results in holoprosencephaly, a syndrome characterized by facial and forebrain abnormalities. Shh null mice have cyclopia and loss of branchial arch structures. It is unclear, however, whether these phenotypes arise solely from the early function of Shh in patterning midline structures, or whether Shh plays other roles in head development.\n\nResults: To address the role of Shh after floorplate induction, we inhibited Shh signaling by injecting hybridoma cells that secrete a function-blocking anti-Shh antibody into the chick cranial mesenchyme. The antibody subsequently bound to Shh in the floorplate, notochord, and the pharyngeal endoderm. Perturbation of Shh signaling at this stage resulted in a significant reduction in head size after 1 day, loss of branchial arch structures after 2 days, and embryos with smaller heads after 7 days. Cell death was significantly increased in the neural tube and neural crest after 1 day, and neural crest cell death was not secondary to the loss of neural tube cells.\n\nConclusions: Reduction of Shh signaling after neural tube closure resulted in a transient decrease in neural tube cell proliferation and an extensive increase in cell death in the neural tube and neural crest, which in turn resulted in decreased head size. The phenotypes observed after reduction of Shh are similar to those observed after cranial neural crest ablation. Thus, our results demonstrate a role for Shh in coordinating the proliferation and survival of cells of the neural tube and cranial neural crest.", "date": "1999-11-18", "date_type": "published", "publication": "Current Biology", "volume": "9", "number": "22", "publisher": "Cell Press", "pagerange": "1304-1314", "id_number": "CaltechAUTHORS:20160323-142432720", "issn": "0960-9822", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-142432720", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS07251" }, { "agency": "American Heart Association", "grant_number": "1168-F1" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" } ] }, "doi": "10.1016/S0960-9822(00)80052-4", "primary_object": { "basename": "mmc1.pdf", "url": "https://authors.library.caltech.edu/records/8yyra-rdg62/files/mmc1.pdf" }, "resource_type": "article", "pub_year": "1999", "author_list": "Ahlgren, Sara C. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nheg5-mhg74", "eprint_id": 8752, "eprint_status": "archive", "datestamp": "2023-08-22 14:06:43", "lastmod": "2023-10-16 21:41:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "Carole" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular mechanisms of neural crest formation", "ispublished": "pub", "full_text_status": "public", "keywords": "BMP, Wnt, FGF, chick, Xenopus", "note": "\"Reprinted, with permission, from the Annual Review of Cell and Developmental Biology, Volume 15 copyright 1999 by Annual Reviews, www.annualreviews.org\" \n\nThe authors thank Dr. CD Stern for communicating data prior to publication and Dr. M Dickinson and members of the laboratory for insightful discussions and comments on the manuscript. This work was supported by grants from the American Cancer Society (CL) and National Institutes of Health NS34671 and NS36585 (MBF).\n\nPublished - LABarcdb99.pdf
", "abstract": "The neural crest is a transient population of multipotent precursor cells named for its site of origin at the crest of the closing neural folds in vertebrate embryos. Following neural tube closure, these cells become migratory and populate diverse regions throughout the embryo where they give rise to most of the neurons and support cells of the peripheral nervous system (PNS), pigment cells, smooth muscle, craniofacial cartilage, and bone. Because of its remarkable ability to generate such diverse derivatives, the neural crest has fascinated developmental biologists for over one hundred years. A great deal has been learned about the migratory pathways neural crest cells follow and the signals that may trigger their differentiation, but until recently comparatively little was known about earlier steps in neural crest development. In the past few years progress has been made in understanding these earlier events, including how the precursors of these multipotent cells are specified in the early embryo and the mechanisms by which they become migratory. In this review, we first examine the mechanisms underlying neural crest induction, paying particular attention to a number of growth factor and transcription factor families that have been implicated in this process. We also discuss when and how the fate of neural crest precursors may diverge from those of nearby neural and epidermal populations. Finally, we review recent advances in our understanding of how neural crest cells become migratory and address the process of neural crest diversification.", "date": "1999-11", "date_type": "published", "publication": "Annual Review of Cell and Developmental Biology", "volume": "15", "publisher": "Annual Reviews", "pagerange": "81-112", "id_number": "CaltechAUTHORS:LABarcdb99", "issn": "1081-0706", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:LABarcdb99", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Cancer Society" }, { "agency": "NIH", "grant_number": "NS34671" }, { "agency": "NIH", "grant_number": "NS36585" } ] }, "doi": "10.1146/annurev.cellbio.15.1.81", "primary_object": { "basename": "LABarcdb99.pdf", "url": "https://authors.library.caltech.edu/records/nheg5-mhg74/files/LABarcdb99.pdf" }, "resource_type": "article", "pub_year": "1999", "author_list": "LaBonne, Carole and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vhret-c5v37", "eprint_id": 65603, "eprint_status": "archive", "datestamp": "2023-09-15 05:19:47", "lastmod": "2023-10-23 21:16:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Ahlgren-S-C", "name": { "family": "Ahlgren", "given": "Sara" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "In Vivo Regulation of Somite Differentiation and Proliferation by Sonic Hedgehog", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Shh; somite; proliferation; BrdU; Pax-1; MyoD; Pax-3", "note": "\u00a9 1999 Academic Press. Received for publication March 16, 1999. Revised June 16, 1999. Accepted June 16, 1999. Available online 2 April 2002.\n\nWe are grateful to the following for their generous gifts of\nreagents used in this study: Dr. Rudy Balling for the Pax-1 probe, Dr. Cliff Tabin for the Shh probe, and Dr. Bruce Patterson for the chick MyoD probe. This work was supported by grants from the Muscular Dystrophy Foundation to C.M. and M.B.F., NIH Grant NSO 7251 to S.A., and NIH Grant 34671 to M.B.F.", "abstract": "In vertebrates, somite differentiation is mediated in part by Sonic Hedgehog (Shh), secreted by the notochord and the floor plate. However, Shh-null mice display close to normal expression of molecular markers for dermomytome, myotome, and sclerotome, indicating that Shh might not be required for their initial induction. In this paper, we have addressed the capacity of Shh to regulate in vivo the expression of the somite differentiation markers Pax-1, MyoD, and Pax-3 after separation of paraxial mesoderm from axial structures. We show that Pax-1, which is lost under these experimental conditions, is rescued by Shh. In contrast, Shh maintains, but cannot induce MyoD expression, while Pax-3 expression is independent of the presence of axial structures or Shh. Finally, we demonstrate that Shh is a potent mitogen for somitic cells, supporting the idea that it may serve to expand subpopulations of cells within the somite.", "date": "1999-10-15", "date_type": "published", "publication": "Developmental Biology", "volume": "214", "number": "2", "publisher": "Elsevier", "pagerange": "277-287", "id_number": "CaltechAUTHORS:20160323-071054297", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-071054297", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Foundation" }, { "agency": "NIH", "grant_number": "NSO 7251" }, { "agency": "NIH", "grant_number": "34671" } ] }, "doi": "10.1006/dbio.1999.9389", "resource_type": "article", "pub_year": "1999", "author_list": "Marcelle, Christophe; Ahlgren, Sara; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/h51gc-b0s31", "eprint_id": 11890, "eprint_status": "archive", "datestamp": "2023-08-22 13:29:39", "lastmod": "2023-10-17 15:57:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Stark-M-R", "name": { "family": "Stark", "given": "Michael R." } }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Competence, specification and induction of Pax-3 in the trigeminal placode", "ispublished": "pub", "full_text_status": "public", "keywords": "Pax-3, Induction, Trigeminal, Placode, Cranial ganglia, Chick", "note": "Copyright \u00a9 1999 by Company of Biologists. \n\nAccepted 19 October; published on WWW 3 December 1998. \n\nWe are very grateful to Dr Andy Groves for discussion and helpful comments on the manuscript. This work was supported by USPHS NS34671 and NS36585 (M.B.F.), and by Human Frontier Science Program Fellowship LT-63/96 and American Heart Association Fellowship 1171-FI1 (C.V.H.B.).\n\nPublished - BAKdev99.pdf
", "abstract": "Placodes are discrete regions of thickened ectoderm that contribute extensively to the peripheral nervous system in the vertebrate head. The paired-domain transcription factor Pax-3 is an early molecular marker for the avian ophthalmic trigeminal (opV) placode, which forms sensory neurons in the ophthalmic lobe of the trigeminal ganglion. Here, we use collagen gel cultures and heterotopic quail-chick grafts to examine the competence, specification and induction of Pax-3 in the opV placode. At the 3-somite stage, the whole head ectoderm rostral to the first somite is competent to express Pax-3 when grafted to the opV placode region, though competence is rapidly lost thereafter in otic-level ectoderm. Pax-3 specification in presumptive opV placode ectoderm occurs by the 8-somite stage, concomitant with robust Pax-3 expression. From the 8-somite stage onwards, significant numbers of cells are committed to express Pax-3. The entire length of the neural tube has the ability to induce Pax-3 expression in competent head ectoderm and the inductive interaction is direct. We propose a detailed model for Pax-3 induction in the opV placode.", "date": "1999-01-01", "date_type": "published", "publication": "Development", "volume": "126", "number": "1", "publisher": "Company of Biologists", "pagerange": "147-156", "id_number": "CaltechAUTHORS:BAKdev99", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BAKdev99", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" }, { "agency": "Human Frontier Science Program", "grant_number": "LT-63/96" }, { "agency": "American Heart Association", "grant_number": "1171-FI1" } ] }, "primary_object": { "basename": "BAKdev99.pdf", "url": "https://authors.library.caltech.edu/records/h51gc-b0s31/files/BAKdev99.pdf" }, "resource_type": "article", "pub_year": "1999", "author_list": "Baker, Clare V. H.; Stark, Michael R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/d1tvg-6bx11", "eprint_id": 63301, "eprint_status": "archive", "datestamp": "2023-08-19 03:47:44", "lastmod": "2024-01-13 16:30:36", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Rostrocaudal differences within the somites confer segmental pattern to trunk neural crest migration", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 2000 Academic Press.", "abstract": "Neural crest cells are one of the most migratory and pleiotropic of embryonic cells types. These cells originate within the central nervous system, but emigrate from the neural tube shortly after its closure. They subsequently migrate along well characterized pathways to populate numerous and diverse derivatives ranging from sensory and autonomic ganglia of the peripheral nervous system to the cartilage and bone of the face. It is reported that neural crest migration is intimately linked to both the formation and segmentation of the somites. After differentiation of somites into dermomyotome and sclerotome, the sclerotomal compartment is subdivided into rostral and caudal halves. Although overtly similar, the caudal half sclerotome has a higher cell density and different molecular markers. Notably, ephrins (Eph) are selectively expressed in the caudal half sclerotome whereas their cognate Eph receptors are expressed on neural crest cells and rostral sclerotomal cells. Functional interactions between Eph receptors and ligands appear to restrict neural crest cells to the rostral half sclerotomal domain. This in turn leads to their segmental migration and the subsequent metameric distribution of neural crest-derived sensory and sympathetic ganglia.", "date": "1999", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "San Diego, CA", "pagerange": "279-96", "id_number": "CaltechAUTHORS:20160101-192122701", "isbn": "0-12-153147-3", "book_title": "Somitogenesis Part 1", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-192122701", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "contributors": { "items": [ { "id": "Ordahl-C-P", "name": { "family": "Ordahl", "given": "Charles P." } } ] }, "doi": "10.1016/S0070-2153(08)60728-0", "resource_type": "book_section", "pub_year": "1999", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/anmyr-92q28", "eprint_id": 16057, "eprint_status": "archive", "datestamp": "2023-08-19 03:38:21", "lastmod": "2023-10-19 21:54:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Garc\u00eda-Castro-M-I", "name": { "family": "Garc\u00eda-Castro", "given": "Martin I." } }, { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Effects of Shh and Noggin on neural crest formation demonstrate that BMP is required in the neural tube but not ectoderm", "ispublished": "pub", "full_text_status": "public", "note": "Copyright \u00a9 1998 by Company of Biologist. \n\nAccepted 30 September; published on WWW 12 November 1998. \n\nWe are indebted to Drs Clare Baker, Meyer Barembaum, Andy Groves, Robb Krumlauf and Carole LaBonne for helpful comments on the manuscript. Many of the reagents used in this study were the kind gifts from colleagues: Noggin-expressing CHO cells were provided by Dr Richard Harland, the Shh retrovirus by Dr Cliff Tabin, the BMP-4 probe used for in situ hybridization by Dr Paul Brickell, the Slug probe Dr Angela Nieto, the Pax-3 probe by Dr Michael Stark, and the Wnt-1 probe by Dr Mary Dickinson. Dr Christophe Marcelle generously assisted with preparation of some of the cells for injection. John Neri kindly helped with the paraffin sectioning and Nikki Pinkerton assisted with in situ hybridizations. M. A. J. S. and M. I. G.-C. performed the experiments reported in this manuscript, K. B. A. helped with the pilot studies and M. B.-F. provided assistance in writing the manuscript, intellectual input and help with cell grafts. M. A. J. S. was supported by a Markey research fellowship and M. I. G.-C. by the American Heart Association (no. 1140-FI1). This work was supported by USPHS NS34671, NS36585 and a grant from the March of Dimes Birth Defects Association.\n\nPublished - SELdev98.pdf
", "abstract": "To define the timing of neural crest formation, we challenged the fate of presumptive neural crest cells by grafting notochords, Sonic Hedgehog- (Shh) or Noggin-secreting cells at different stages of neurulation in chick embryos. Notochords or Shh-secreting cells are able to prevent neural crest formation at open neural plate levels, as assayed by DiI-labeling and expression of the transcription factor, Slug, suggesting that neural crest cells are not committed to their fate at this time. In contrast, the BMP signaling antagonist, Noggin, does not repress neural crest formation at the open neural plate stage, but does so if injected into the lumen of the closing neural tube. The period of Noggin sensitivity corresponds to the time when BMPs are expressed in the dorsal neural tube but are down-regulated in the non-neural ectoderm. To confirm the timing of neural crest formation, Shh or Noggin were added to neural folds at defined times in culture. Shh inhibits neural crest production at early stages (0-5 hours in culture), whereas Noggin exerts an effect on neural crest production only later (5-10 hours in culture). Our results suggest three phases of neurulation that relate to neural crest formation: (1) an initial BMP-independent phase that can be prevented by Shh-mediated signals from the notochord; (2) an intermediate BMP-dependent phase around the time of neural tube closure, when BMP-4 is expressed in the dorsal neural tube; and (3) a later pre-migratory phase which is refractory to exogenous Shh and Noggin.", "date": "1998-12-15", "date_type": "published", "publication": "Development", "volume": "125", "number": "24", "publisher": "Company of Biologists", "pagerange": "4919-4930", "id_number": "CaltechAUTHORS:20090925-103910673", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090925-103910673", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Lucille P. Markey Charitable Trust" }, { "agency": "American Heart Association", "grant_number": "1140-FI1" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" }, { "agency": "March of Dimes Birth Defects Association" } ] }, "primary_object": { "basename": "SELdev98.pdf", "url": "https://authors.library.caltech.edu/records/anmyr-92q28/files/SELdev98.pdf" }, "resource_type": "article", "pub_year": "1998", "author_list": "Selleck, Mark A. J.; Garc\u00eda-Castro, Martin I.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6hs41-6cs38", "eprint_id": 65594, "eprint_status": "archive", "datestamp": "2023-08-19 03:32:24", "lastmod": "2023-10-18 16:09:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ruffins-S", "name": { "family": "Ruffins", "given": "Seth" } }, { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin Bruk" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Early Migrating Neural Crest Cells Can Form Ventral Neural Tube Derivatives When Challenged by Transplantation", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1998 Academic Press. Received for publication April 14, 1998, Revised May 26 1998, Accepted June 2 1998, Available online 9 April 2002.\n\nWe thank Clare Baker for helpful comments on the manuscript.\nThis work was supported by USPHS NS34671 and NS36585.", "abstract": "Once neural crest cells undergo an epithelial\u2013mesenchymal transition to leave the neural tube, it has been classically assumed that they are fated to differentiate within the neural crest lineage. To test this idea, we challenged the developmental potential of recently emigrated neural crest cells by transplanting them into the ventral portion of the neural tube at the open neural plate stage. Newly migrating neural crest cells were isolated in tissue culture, labeled with the lipophilic dye DiI, and microinjected into the ventral portion of the neural plate. After 2 days, some neural crest cells became incorporated into the neuroepithelium in positions characteristic of floor plate cells and motor neurons. Some of the labeled cells within the ventral neural tube expressed FP-1, characteristic of floor plate cells. A few labeled cells were found in positions characteristic of motor neurons and expressed islet-1. In contrast, neural crest cells transplanted onto neural crest pathways expressed the HNK-1 epitope, but no ventral neural tube markers. Injection of neural crest cells into the mesenchyme adjacent to the notochord or culturing them in the presence of Sonic hedgehog failed to elicit FP-1 expression. These results suggest that migrating neural crest cells are flexible in their fate and retain the ability to form neural tube derivatives even after emigrating from the neural tube.", "date": "1998-11-15", "date_type": "published", "publication": "Developmental Biology", "volume": "203", "number": "2", "publisher": "Elsevier", "pagerange": "295-304", "id_number": "CaltechAUTHORS:20160322-115803598", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-115803598", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" } ] }, "doi": "10.1006/dbio.1998.8973", "resource_type": "article", "pub_year": "1998", "author_list": "Ruffins, Seth; Artinger, Kristin Bruk; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/38r0z-fsv95", "eprint_id": 65638, "eprint_status": "archive", "datestamp": "2023-08-19 03:29:09", "lastmod": "2023-10-18 16:12:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Wolf-J-J", "name": { "family": "Wolf", "given": "John" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Age-Dependent Neurotransmitter Plasticity of Ciliary Ganglion Neurons", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1998 Academic Press. Received August 20, 1998. Revised September 25, 1998. Accepted September 28, 1998. Available online 25 May 2002.\n\nWe thank Dr. J. Coulombe for advice and preliminary assistance with cell purification procedures and Dr. Sara Ahlgren for input on apoptosis. This work was supported by NS 36585 and NS34671.", "abstract": "We have examined neurotransmitter plasticity in postmitotic cholinergic neurons isolated from 6.5- to 11-day-old embryonic quail ciliary ganglia. Purified neurons were labeled with DiI, transplanted into the trunk of young chick embryos, and assayed for catecholamine content and [^3H]thymidine uptake 4 to 5 days later. For ciliary neurons derived from 6.5- to 8-day-old embryos, as many as 25% (average of 9% overall) expressed catecholamines in the host sympathetic ganglia, migratory stream, aortic plexuses, and adrenal medulla. In contrast, neurons from >8-day-old ganglia did not acquire or produce detectable catecholamines, indicating a limited time period over which phenotypic conversion can occurin vivo.As a control, ciliary neurons were also injected into the head mesenchyme of young embryos; no catecholamine expression was observed. Interestingly, after transplantation some Dil-labeled postmitotic ciliary neurons took up [^3H]thymidine with or without phenotypic change. These results suggest that phenotypic plasticity in ciliary neurons is age-dependent, is location-dependent, and may involve resumption of DNA replication, a characteristic feature of some differentiating adrenergic sympathetic neurons. Apoptosis of a few proliferating transplanted cells may be induced independently or in association with transmitter change.", "date": "1998-11", "date_type": "published", "publication": "Molecular and Cellular Neuroscience", "volume": "12", "number": "4-5", "publisher": "Elsevier", "pagerange": "311-323", "id_number": "CaltechAUTHORS:20160324-065910513", "issn": "1044-7431", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160324-065910513", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "NS 36585" }, { "agency": "NIH", "grant_number": "NS34671" } ] }, "doi": "10.1006/mcne.1998.0720", "resource_type": "article", "pub_year": "1998", "author_list": "Sechrist, John W.; Wolf, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4g81b-med79", "eprint_id": 65595, "eprint_status": "archive", "datestamp": "2023-08-19 03:23:40", "lastmod": "2023-10-18 16:09:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kil-Sung-H", "name": { "family": "Kil", "given": "Sung H." } }, { "id": "Krull-C-E", "name": { "family": "Krull", "given": "Catherine E." } }, { "id": "Cann-G-L", "name": { "family": "Cann", "given": "Gordon" } }, { "id": "Clegg-D", "name": { "family": "Clegg", "given": "Dennis" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The \u03b1_4 Subunit of Integrin Is Important for Neural Crest Cell Migration", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1998 Academic Press. Received for publication April 20, 1998. Revised June 11, 1998. Accepted June 12, 1998. Available online 15 April 2002.\n\nWe thank Drs. Arthur Lander, Diane O'Dowd, Ben Murray, and\nMartin Garcia-Castro for critical comments on the manuscript and constructive discussion during the course of this work. This study was supported by USPHS HD15527 and a grant from the Muscular Dystrophy Foundation to M.B.F.", "abstract": "We identify the \u03b1_4 subunit of integrin as a predominant integrin expressed by neural crest cells in both avian and murine embryos. Using degenerate primers, we obtained a PCR fragment of the chick integrin \u03b1_4 subunit that was subsequently used to clone the full-length subunit with a predicted amino acid sequence 60% identical to human and mouse \u03b1_4 subunits.In situhybridization demonstrates that chick integrin \u03b1_4 mRNA is expressed at high levels by migrating neural crest cells and neural crest-derived ganglia at both cranial and trunk levels. An antibody against the murine \u03b1_4 subunit revealed similar distribution patterns in mouse to chick. In addition to neural crest cells, the integrin \u03b1_4 subunit was later observed on the muscle masses of the limb, the apical ectodermal ridge, and the developing liver. To examine the functional role of the integrin \u03b1_4 subunit in neural crest cell migration, we used an explant preparation that allows visualization of neural crest cells in their normal environment with or without perturbing reagents. In the presence of a blocking antibody against the mouse integrin \u03b1_4 subunit, there was a profound abrogation of neural crest cell migration at trunk and hindbrain levels. Both the numbers of migrating neural crest cells and the total distance traversed were markedly reduced. Similarly, avian embryos injected with synthetic peptides that contain the integrin \u03b1_4 binding site in fibronectin displayed abnormal neural crest cell migration. Our results suggest that the integrin \u03b1_4 subunit is important for normal neural crest cell migration and may be one of the primary \u03b1 subunits used for neural crest cell migrationin vivo.Furthermore, the integrin \u03b1_4 subunit represents a useful neural crest marker in the mouse.", "date": "1998-10-01", "date_type": "published", "publication": "Developmental Biology", "volume": "202", "number": "1", "publisher": "Elsevier", "pagerange": "29-42", "id_number": "CaltechAUTHORS:20160322-120549369", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-120549369", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD15527" }, { "agency": "Muscular Dystrophy Foundation" } ] }, "doi": "10.1006/dbio.1998.8985", "resource_type": "article", "pub_year": "1998", "author_list": "Kil, Sung H.; Krull, Catherine E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ky7gx-xmf75", "eprint_id": 65814, "eprint_status": "archive", "datestamp": "2023-08-22 13:07:59", "lastmod": "2023-10-18 16:49:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Fedtsova-N", "name": { "family": "Fedtsova", "given": "Natalia" } }, { "id": "Rhee-J-M", "name": { "family": "Rhee", "given": "Jerry M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Turner-E-E", "name": { "family": "Turner", "given": "Eric" } } ] }, "title": "Placodal Origin of Brn-3\u2014Expressing Cranial Sensory Neurons", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Brn-3; sensory placode; trigeminal ganglion; POU domain; neural crest", "note": "\u00a9 1998 John Wiley & Sons, Inc. \n\nReceived 5 March 1998; accepted 4 May 1998. \n\nTue authors are indebted to Dr. John Sechrist for his participation in the experiments and extensive review of the manuscript. Eric E. Turner and Natalia Fedtsova are NARSAD Young Investigators, and NF was supported in part by NIH Training Grant. 5-T32-MH19934. This work was suppo11ed in part by the Pfizer New Faculty Award, Scottish Rite Schizophrenia Research Program, Department of Veterans Affairs MERIT funding, and USPHS awards HD33442 and MH58447 to EET and USPHS awards NS36585 and NS34671 to MBF.", "abstract": "The Brn-3 class of POU-domain transcription factors includes three genes in mammals which have key roles in the development of specific groups of sensory neurons. Here, we have identified three avian genes which correspond to the murine genes Brn-3.0, Brn-3.1, and Brn-3.2. Using an in situ hybridization probe generic for this gene class, the earliest detectable expression of Brn-3 in the chick is at stage 15, in placodal and migrating precursors of the trigeminal ganglion. By stage 19, Brn-3.0 protein is detectable in the trigeminal and vestibulocochlear ganglia with Brn-3.0-specific antisera, and Brn-3 message expression has extended to the dorsal root ganglia. At later stages, when condensation of the trigeminal ganglion is complete, Brn-3.0-immunoreactive neurons are concentrated in the portion of the ganglion distal to the brain stem. To examine the developmental origin of the Brn-3 expressing cells, we combined lipophilic dye (DiI) labeling with in situ hybridization. DiI labeling of the placodal surface ectoderm and of premigratory neural crest cells in the neural tube reveals that all, or nearly all, of the Brn-3-expressing neurons in the trigeminal ganglia are derived from the sensory placodes and not from the neural crest, and thus, that Brn-3 is an early marker of the placode-derived sensory neural lineage.", "date": "1998-09-15", "date_type": "published", "publication": "Journal of Neurobiology", "volume": "36", "number": "4", "publisher": "Wiley", "pagerange": "572-585", "id_number": "CaltechAUTHORS:20160331-142049577", "issn": "0022-3034", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160331-142049577", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "5-T32-MH19934" }, { "agency": "Pfizer" }, { "agency": "Scottish Rite Schizophrenia Research Program" }, { "agency": "Department of Veterans Affairs" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD33442" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "MH58447" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS34671" }, { "agency": "National Alliance for Research on Schizophrenia and Depression (NARSAD)" } ] }, "doi": "10.1002/(SICI)1097-4695(19980915)36:4<572::AID-NEU10>3.0.CO;2-A", "resource_type": "article", "pub_year": "1998", "author_list": "Artinger, Kristin B.; Fedtsova, Natalia; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jyv6t-7k587", "eprint_id": 52095, "eprint_status": "archive", "datestamp": "2023-08-19 03:14:13", "lastmod": "2023-10-18 19:33:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Martinsen-B-J", "name": { "family": "Martinsen", "given": "Brad J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural Crest Specification Regulated by the Helix-Loop-Helix Repressor Id2", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1998 American Association for the Advancement of Science.\n\nReceived 16 March 1998; accepted 1 July 1998.\n\nWe thank S. Fraser, C. Baker, and M. Dickinson for\ncritical reading of the manuscript; J. Sechrist for\ncritical analysis of ectoderm conversion data; C. Marcelle,\nT. Moreno, M. Barembaum, and C. LaBonne for\nadvice on molecular biology protocols; and K. Min\nand J. Neri for cryostat sectioning. We are grateful to\nS. Bryant and D. Gardiner for the stage 13 to 24 chick\nembryo library. Supported by U.S. Public Health Service\ngrants NS34671 and NS36585.", "abstract": "Vertebrate neural crest cells, derived from the neural folds, generate a variety of tissues, such as cartilage, ganglia, and cranial (intramembranous) bone. The chick homolog of the helix-loop-helix transcriptional regulator Id2 is expressed in cranial but not trunk neural folds and subsequently in some migrating cranial neural crest cells. Ectopic expression of Id2 with recombinant retroviruses converted ectodermal cells to a neural crest fate, demonstrating that proper regulation of Id2 is important for sustaining epidermal traits. In addition, overexpression of Id2 resulted in overgrowth and premature neurogenesis of the dorsal neural tube. These results suggest that Id2 may allocate ectodermal precursors into neural rather than epidermal lineages.", "date": "1998-08-14", "date_type": "published", "publication": "Science", "volume": "281", "number": "5379", "publisher": "American Association for the Advancement of Science", "pagerange": "988-991", "id_number": "CaltechAUTHORS:20141124-104739069", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20141124-104739069", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS36585" } ] }, "doi": "10.1126/science.281.5379.988", "resource_type": "article", "pub_year": "1998", "author_list": "Martinsen, Brad J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8tsby-7yy09", "eprint_id": 15982, "eprint_status": "archive", "datestamp": "2023-08-22 12:59:40", "lastmod": "2023-10-19 21:21:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "Carole" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest induction in Xenopus: evidence for a two-signal model", "ispublished": "pub", "full_text_status": "public", "keywords": "Neural crest; Xenopus; Slug; BMP; Wnt; FGF; Chordin; Molecular interaction", "note": "Copyright \u00a9 1998 by Company of Biologists. \n\nAccepted 7 April; published on WWW 3 June 1998. \n\nThe authors gratefully acknowledge A. Collazo, A. Knecht, C. Baker, S. Fraser and T. Moreno for critical reading of the manuscript; A. Gross and members of the laboratory for helpful comments and suggestions; and Eddy DeRobertis, Randy Moon, Rob Grainger, Gerry Thompsen, Jonathan Slack, Robert Davis, Peter Vize, Doug Melton and Roberto Mayor for providing reagents. C. L. is a fellow of the American Cancer Society. This work was also supported by USPHS NS36585 and NS34671 to M. B. F.\n\nPublished - LABdev98.pdf
", "abstract": "We have investigated the molecular interactions underlying neural crest formation in Xenopus. Using chordin overexpression to antagonize endogenous BMP signaling in whole embryos and explants, we demonstrate that such inhibition alone is insufficient to account for neural crest induction in vivo. We find, however, that chordin-induced neural plate tissue can be induced to adopt neural crest fates by members of the FGF and Wnt families, growth factors that have previously been shown to posteriorize induced neural tissue. Overexpression of a dominant negative XWnt-8 inhibits the expression of neural crest markers, demonstrating the necessity for a Wnt signal during neural crest induction in vivo. The requirement for Wnt signaling during neural crest induction is shown to be direct, whereas FGF-mediated neural crest induction may be mediated by Wnt signals. Overexpression of the zinc finger transcription factor Slug, one of the earliest markers of neural crest formation, is insufficient for neural crest induction. Slug-expressing ectoderm will generate neural crest in the presence of Wnt or FGF-like signals, however, bypassing the need for BMP inhibition in this process. A two-step model for neural crest induction is proposed.", "date": "1998-07-01", "date_type": "published", "publication": "Development", "volume": "125", "number": "13", "publisher": "Company of Biologists", "pagerange": "2403-2414", "id_number": "CaltechAUTHORS:20090918-134232858", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20090918-134232858", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "American Cancer Society" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS34671" } ] }, "primary_object": { "basename": "LABdev98.pdf", "url": "https://authors.library.caltech.edu/records/8tsby-7yy09/files/LABdev98.pdf" }, "resource_type": "article", "pub_year": "1998", "author_list": "LaBonne, Carole and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qcfjf-55n56", "eprint_id": 65810, "eprint_status": "archive", "datestamp": "2023-08-22 12:34:43", "lastmod": "2023-10-18 16:49:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "LaBonne-C", "name": { "family": "LaBonne", "given": "Carole" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Induction and Patterning of the Neural Crest, a Stem Cell-Like Precursor Population", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest; stem cells; lineage determination; avian; Xenopus", "note": "\u00a9 1998 John Wiley & Sons, Inc. \n\nReceived 15 January 1998, accepted 31 March 1998. \n\nThe authors thank Clare Baker and Andrew Groves for comments in the manuscript and Ali Hemmati-Brivanlov for communicating data prior to publication.", "abstract": "The neural crest is a multipotent precursor population which ulimately generates much of the peripheral nervous system, epidermal pigment cells, and a variety of mesectodermal derivatives. Individual multipotent neural crest cells are capable of some self-renewing divisions, and based upon this criteria can be considered stem cells. Considerable progress has been made in recent years toward understanding how this important population of progenitor cells is initially established in the early embryo, and how cell-intrinsic and non-cell-instristic factors mediate their subsequent lineage segregation and differentiation.", "date": "1998-01-09", "date_type": "published", "publication": "Journal of Neurobiology", "volume": "36", "number": "2", "publisher": "Wiley", "pagerange": "175-189", "id_number": "CaltechAUTHORS:20160331-132034521", "issn": "0022-3034", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160331-132034521", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/(SICI)1097-4695(199808)36:2<175::AID-NEU6>3.0.CO;2-Z", "resource_type": "article", "pub_year": "1998", "author_list": "LaBonne, Carole and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3mf1c-za230", "eprint_id": 63299, "eprint_status": "archive", "datestamp": "2023-08-19 02:13:42", "lastmod": "2023-10-25 23:42:46", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Groves-A-K", "name": { "family": "Groves", "given": "Andrew K." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest diversification", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 1999 Academic Press. \n\nWe are very grateful to Dr. Clare Baker for her many helpful suggestions and comments on the manuscript, and for providing us with an invaluable list of references. A.K.G. is supported by NIH Grant RO3 DC03630-01. Work in our laboratory was supported by NIH Grants NS36585 and NS34671.", "abstract": "This chapter discusses some of the basic principles that have emerged from the study of neural crest biology. It discusses the origin of the neural crest in the embryo and considers some of the general principles underlying neural crest diversification. Finally, the chapter discusses what is known about how specific derivatives of the neural crest are generated. The neural crest has captured the imagination of developmental biologists for over a century. There are two principal reasons for this. First, it is a highly migratory cell population and very little is known about the mechanisms that precisely guide neural crest cells to specific sites in the embryo. Second, the neural crest gives rise to an enormous diversity of cell types, including the entire peripheral nervous system, melanocytes, endocrine cells, and much of the connective tissue, bone, and cartilage of the face and skull. Neural crest cells arise at the junction between the prospective epidermis and the prospective neural plate in all vertebrates, regardless of whether the neural crest ultimately migrates from the open neural folds, the closed neural tube, or an ectodermal thickening at the neural plate-epidermis boundary. Although a number of models have been proposed for how neural crest cells are generated, the weight of evidence at present suggests that neural crest arises from local cell-cell interactions between the epidermis and the neural plate.", "date": "1998", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "San Diego, CA", "pagerange": "221-258", "id_number": "CaltechAUTHORS:20160101-191412413", "isbn": "978-0-12-153143-0", "book_title": "Current Topics in Developmental Biology", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-191412413", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RO3 DC03630-01" }, { "agency": "NIH", "grant_number": "NS36585" }, { "agency": "NIH", "grant_number": "NS34671" } ] }, "contributors": { "items": [ { "id": "Pedersen-R-A", "name": { "family": "Pedersen", "given": "Roger A." } }, { "id": "Schatten-G-P", "name": { "family": "Schatten", "given": "Gerald P." } } ] }, "doi": "10.1016/S0070-2153(08)60383-X", "resource_type": "book_section", "pub_year": "1998", "author_list": "Groves, Andrew K. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3kpem-4z793", "eprint_id": 65633, "eprint_status": "archive", "datestamp": "2023-08-19 02:04:57", "lastmod": "2023-10-18 16:12:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Differentiation of the vertebrate neural tube", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1997 Current Biology Ltd. Available online 4 July 2002.\n\nWe thank Andrew Groves and Anne Knecht for helpful comments on the manuscript.", "abstract": "The vertebrate nervous system arises through a series of inductive interactions, beginning with the induction of the neural plate and the rostrocaudal patterning of the neural tube. The process continues with dorsoventral patterning of the neural tube, during which floor plate cells and motor neurons are induced ventrally by interactions of the neural tube with the notochord, and dorsal cell types are induced via neural plate/ectodermal interactions. Later interactions result in the formation of interneurons as well as neuronal migrations. Recent progress, guided in part by knowledge of evolutionary conservation of transcription factors and signaling pathways, is beginning to reveal the cellular and molecular bases of each of these steps in neuronal patterning.", "date": "1997-12", "date_type": "published", "publication": "Current Opinion in Cell Biology", "volume": "9", "number": "6", "publisher": "Elsevier", "pagerange": "885-891", "id_number": "CaltechAUTHORS:20160323-142240057", "issn": "0955-0674", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-142240057", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0955-0674(97)80092-0", "resource_type": "article", "pub_year": "1997", "author_list": "Bronner-Fraser, Marianne and Fraser, Scott E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wrzvv-9h497", "eprint_id": 65632, "eprint_status": "archive", "datestamp": "2023-09-15 05:20:05", "lastmod": "2023-10-23 21:16:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The origins of the neural crest. Part I: embryonic induction", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; Induction; Xenopus; Chick; BMP; FGF", "note": "\u00a9 1997 Elsevier Science Ireland Ltd. Received 15 May 1997; revised version received 11 August 1997; accepted 11 August 1997. Available online 19 February 1998.\n\nThe authors are indebted to Drs Carole LaBonne and\nMary Dickinson for insightful discussion and for critical\nreading of the manuscript.", "abstract": "Neural crest cells form at the border between the neural plate and the epidermis. The tissue interactions that underlie neural crest cell induction have been investigated primarily by heterotopic grafting experiments in vivo and by conjugating different tissues in vitro. Three models have been proposed to explain the induction of neural crest cells at the neural plate border, i.e. (1) the influence of signals from the mesoderm, (2) changes in ectodermal competence and (3) local interactions between neural and non-neural ectoderm. The weight of the evidence supports the last model, although there are data that suggest a role for signals from the mesoderm. FGFs seem to be necessary but not sufficient for neural crest cell induction. BMP-4 is sufficient to induce neural crest cells from chick neural explants in vitro and intermediate levels of BMP-4-signalling induce neural crest cell markers in Xenopus animal cap assays. These data suggest a gradient model in which neural crest cells are induced by a particular range of BMP-4 activity, although a single-signal model may be too simplistic. Neural crest cell induction may be an ongoing process, in which an initial induction at the neural plate border is followed by further induction within the dorsal neural tube.", "date": "1997-12", "date_type": "published", "publication": "Mechanisms of Development", "volume": "69", "number": "1-2", "publisher": "Elsevier", "pagerange": "3-11", "id_number": "CaltechAUTHORS:20160323-142239679", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-142239679", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0925-4773(97)00132-9", "resource_type": "article", "pub_year": "1997", "author_list": "Baker, Clare V. H. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tw0er-rc396", "eprint_id": 65631, "eprint_status": "archive", "datestamp": "2023-09-15 05:19:56", "lastmod": "2023-10-23 21:16:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The origins of the neural crest. Part II: an evolutionary perspective", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; Placode; Evolution; Chordate; Vertebrate; Amphioxus; Ascidian", "note": "\u00a9 1997 Elsevier Science Ireland Ltd. Received 15 May 1997; revised version received 11 August 1997; accepted 11 August 1997. Available online 19 February 1998.\n\nThe authors would like to thank Drs Kristin Artinger,\nAjay Chitnis, Wolfgang Driever, James Langeland, Michael\nLevine, Michael Thorndyke and Hiroshi Wada for communicating\ndata prior to publication. We are indebted to Drs\nThurston Lacalli, Kevin Peterson and Andres Collazo for\ntheir insights and for stimulating discussions. We thank Drs\nAndres Collazo, Kevin Peterson and Andrew Groves for\ntheir patient and critical reading of numerous drafts of the\nmanuscript.", "abstract": "The neural crest and cranial ectodermal placodes are traditionally thought to be unique to vertebrates; however, they must have had evolutionary precursors. Here, we review recent evidence suggesting that such ancestral cell types can be identified in modern non-vertebrate chordates, such as amphioxus (a cephalochordate) and ascidians (urochordates). Hence, migratory neuroectodermal cells may well have been present in the common ancestor of the chordates, such that the possibility of their existence in non-chordate deuterostomes (hemichordates and echinoderms) must also be considered. Finally, we discuss the various non-neuronal cell types produced by the neural crest in order to demonstrate that it is plausible that these different cell types evolved from an ancestral population that was neuronal in nature.", "date": "1997-12", "date_type": "published", "publication": "Mechanisms of Development", "volume": "69", "number": "1-2", "publisher": "Elsevier", "pagerange": "13-29", "id_number": "CaltechAUTHORS:20160323-142239375", "issn": "0925-4773", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-142239375", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0925-4773(97)00129-9", "resource_type": "article", "pub_year": "1997", "author_list": "Baker, Clare V. H. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/76npv-7j389", "eprint_id": 28850, "eprint_status": "archive", "datestamp": "2023-08-22 11:59:23", "lastmod": "2023-10-24 18:11:36", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stark-M-R", "name": { "family": "Stark", "given": "Michael R." } }, { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural tube-ectoderm interactions are required for trigeminal placode formation", "ispublished": "pub", "full_text_status": "public", "keywords": "placode; cranial ganglion; neural crest; Pax-3; FREK; chick", "note": "\u00a9 1997 The Company of Biologists Limited. \n\nAccepted 11 August 1997. \n\nWe thank Angela Nieto for early contributions to this work and Martin Goulding for the chick and mouse Pax-3 probe. We are indebted to Brian Rowe, Roham Zamanian, Johnny Choi, and Parisa Zarbafian for technical assistance and Drs Clare Baker, Andrew Groves, Catherine Krull and Ben Murray for critical reading of the manuscript. This work was supported by NS34671 to M. B. F. and a grant from the Muscular Dystrophy Association to C. M.\n\nPublished - STAdev97.pdf
", "abstract": "Cranial sensory ganglia in vertebrates develop from the ectodermal placodes, the neural crest, or both. Although much is known about the neural crest contribution to cranial ganglia, relatively little is known about how placode cells form, invaginate and migrate to their targets. Here, we identify Pax-3 as a molecular marker for placode cells that contribute to the ophthalmic branch of the trigeminal ganglion and use it, in conjunction with DiI labeling of the surface ectoderm, to analyze some of the mechanisms underlying placode development. Pax-3 expression in the ophthalmic placode is observed as early as the 4-somite stage in a narrow band of ectoderm contiguous to the midbrain neural folds. Its expression broadens to a patch of ectoderm adjacent to the midbrain and the rostral hindbrain at the 8- to 10-somite stage. Invagination of the first Pax-3-positive cells begins at the 13-somite stage. Placodal invagination continues through the 35-somite stage, by which time condensation of the trigeminal ganglion has begun. To challenge the normal tissue interactions leading to placode formation, we ablated the cranial neural crest cells or implanted barriers between the neural tube and the ectoderm. Our results demonstrate that, although the presence of neural crest cells is not mandatory for Pax-3 expression in the forming placode, a diffusible signal from the neuroectoderm is required for induction and/or maintenance of the ophthalmic placode.", "date": "1997-11", "date_type": "published", "publication": "Development", "volume": "124", "number": "21", "publisher": "Company of Biologists", "pagerange": "4287-4295", "id_number": "CaltechAUTHORS:20120119-094014765", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120119-094014765", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34671" }, { "agency": "Muscular Dystrophy Association" } ] }, "primary_object": { "basename": "STAdev97.pdf", "url": "https://authors.library.caltech.edu/records/76npv-7j389/files/STAdev97.pdf" }, "resource_type": "article", "pub_year": "1997", "author_list": "Stark, Michael R.; Sechrist, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3jspy-6ft15", "eprint_id": 28847, "eprint_status": "archive", "datestamp": "2023-08-22 11:55:32", "lastmod": "2023-10-24 18:11:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Stark-M-R", "name": { "family": "Stark", "given": "Michael R." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Coordinate actions of BMPs, Wnts, Shh and noggin mediate patterning of the dorsal somite", "ispublished": "pub", "full_text_status": "public", "keywords": "somite; dermomyotome; BMP; Sonic Hedgehog; Wnt; noggin; chick", "note": "\u00a9 1997 The Company of Biologists Limited. \n\nAccepted 1 August 1997. \n\nWe thank Drs Charles Ordahl and Scott Fraser for critical reading of the manuscript and Johnny Choi and Sung Hee Kil for their help during this research. We are grateful to the following for their generous gifts of reagents used in this study: Dr Paul Brickell for the\nchick BMP-4 probe used for in situ hybridization and the mouse BMP-4 cDNA cloned in the RCAS(A) retroviral vector, Dr Richard Harland for the noggin-secreting CHO cells, Dr Cliff Tabin for the chick Sonic hedgehog cDNA cloned in the RCAS(A) retroviral vector and for the chick Patched probe, Dr Bruce Patterson for the chick MyoD probe, Dr Anthony Brown for the Wnt-1-expressing cell line and Dr Jonathan Cooke for the chick noggin probe. This work was\nsupported by grants from the Muscular Dystrophy Foundation to C. M. and M. B. F.\n\nPublished - MARdev97.pdf
", "abstract": "Shortly after their formation, somites of vertebrate embryos\ndifferentiate along the dorsoventral axis into sclerotome,\nmyotome and dermomyotome. The dermomyotome is then\npatterned along its mediolateral axis into medial, central\nand lateral compartments, which contain progenitors of\nepaxial muscle, dermis and hypaxial muscle, respectively.\nHere, we used Wnt-11 as a molecular marker for the medial\ncompartment of dermomyotome (the 'medial lip') to\ndemonstrate that BMP in the dorsal neural tube indirectly\ninduces formation of the medial lip by up-regulating Wnt-1\nand Wnt-3a (but not Wnt-4) expression in the neural tube.\nNoggin in the dorsal somite may inhibit the direct action of\nBMP on this tissue. Wnt-11 induction is antagonized by\nSonic Hedgehog, secreted by the notochord and the floor\nplate. Together, our results show that the coordinated\nactions of the dorsal neural tube (via BMP and Wnts), the\nventral neural tube/notochord (via Shh) and the somite\nitself (via noggin) mediates patterning of the dorsal compartment of the somite.", "date": "1997-10", "date_type": "published", "publication": "Development", "volume": "124", "number": "20", "publisher": "Company of Biologists", "pagerange": "3955-3963", "id_number": "CaltechAUTHORS:20120119-093000980", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120119-093000980", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Foundation" } ] }, "primary_object": { "basename": "MARdev97.pdf", "url": "https://authors.library.caltech.edu/records/3jspy-6ft15/files/MARdev97.pdf" }, "resource_type": "article", "pub_year": "1997", "author_list": "Marcelle, Christophe; Stark, Michael R.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/7qhjp-j2x91", "eprint_id": 11888, "eprint_status": "archive", "datestamp": "2023-08-22 11:52:22", "lastmod": "2023-10-17 15:57:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Baker-C-V-H", "name": { "family": "Baker", "given": "Clare V. H." } }, { "id": "Le-Douarin-N-M", "name": { "family": "Le Douarin", "given": "Nicole M." } }, { "id": "Teillet-M-A", "name": { "family": "Teillet", "given": "Marie-Aim\u00e9e" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Early- and late-migrating cranial neural crest cell populations have equivalent developmental potential in vivo", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, chick, quail, cell migration, cell fate", "note": "Copyright \u00a9 1997 by Company of Biologists. \n\n(Accepted 4 May 1997) \n\nThanks to Delphine Champeval and Claude Oudin for technical assistance, and to Yann Rantier for photographs of embryos. Thanks to Michael Rodrigues for suggesting the early ablation, late grafting experiment, and to Andy Groves and Mark Selleck for helpful comments on the manuscript. This work was supported by Wellcome Trust Travelling Research Fellowship 043093/Z/94/Z, and by Human Frontier Science Program Fellowship LT-63/96.\n\nPublished - BAKdev97.pdf
", "abstract": "We present the first in vivo study of the long-term fate and potential of early-migrating and late-migrating mesencephalic neural crest cell populations, by performing isochronic and heterochronic quail-to-chick grafts. Both early- and late-migrating populations form melanocytes, neurons, glia, cartilage and bone in isochronic, isotopic chimeras, showing that neither population is lineage-restricted. The early-migrating population distributes both dorsally and ventrally during normal development, while the late-migrating population is confined dorsally and forms much less cartilage and bone. When the late-migrating population is substituted heterochronically for the early-migrating population, it contributes extensively to ventral derivatives such as jaw cartilage and bone. Conversely, when the early-migrating population is substituted heterochronically for the late-migrating population, it no longer contributes to the jaw skeleton and only forms dorsal derivatives. When the late-migrating population is grafted into a late-stage host whose neural crest had previously been ablated, it migrates ventrally into the jaws. Thus, the dorsal fate restriction of the late-migrating mesencephalic neural crest cell population in normal development is due to the presence of earlier-migrating neural crest cells, rather than to any change in the environment or to any intrinsic difference in migratory ability or potential between early- and late-migrating cell populations. These results highlight the plasticity of the neural crest and show that its fate is determined primarily by the environment.", "date": "1997-08-15", "date_type": "published", "publication": "Development", "volume": "124", "number": "16", "publisher": "Company of Biologists", "pagerange": "3077-3087", "id_number": "CaltechAUTHORS:BAKdev97", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BAKdev97", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Wellcome Trust", "grant_number": "043093/Z/94/Z" }, { "agency": "Human Frontier Science Program", "grant_number": "LT-63/96" } ] }, "primary_object": { "basename": "BAKdev97.pdf", "url": "https://authors.library.caltech.edu/records/7qhjp-j2x91/files/BAKdev97.pdf" }, "resource_type": "article", "pub_year": "1997", "author_list": "Baker, Clare V. H.; Le Douarin, Nicole M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/gkqv8-wk811", "eprint_id": 65630, "eprint_status": "archive", "datestamp": "2023-08-19 01:42:07", "lastmod": "2023-10-18 16:12:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krull-C-E", "name": { "family": "Krull", "given": "Catherine E." } }, { "id": "Lansford-Rusty-D", "name": { "family": "Lansford", "given": "Rusty" }, "orcid": "0000-0002-2159-3699" }, { "id": "Gale-N-W", "name": { "family": "Gale", "given": "Nicholas W." } }, { "id": "Collazo-A", "name": { "family": "Collazo", "given": "Andres" } }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Yancopoulos-G-D", "name": { "family": "Yancopoulos", "given": "George D." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1997 Current Biology Ltd. Received: 28 May 1997. Revised: 23 June 1997. Accepted: 23 June 1997. Published: 11 July 1997. Available online 10 April 2004.\n\nThe first two authors contributed equally to this study. We thank J. Neri and P. Godoy for technical assistance, S. Ruffins for invaluable help with preparing figures, and M. Dickinson, D. Wilkinson, D. Anderson for helpful\ncomments on the manuscript and for sharing results before publication. We thank D. Kenny for help with obtaining the full-length QEK10 probe. We thank M. Henkenmeyer and T. Pawson for providing anti-EphB2 antibody and H. Wang and D. Anderson for providing the ephrin-B2 (Htk-L) and ephrin-B1 (Elk-L) probes. We thank J. Solomon and S. Speicher for\nproviding the cell tracking program used in this study. This work was supported by USPHS HD-15527 and a grant from the Muscular Dystrophy Association to M.B.F. and MH-49176 to S.E.F.", "abstract": "Background: In the trunk of avian embryos, neural crest migration through the somites is segmental, with neural crest cells entering the rostral half of each somitic sclerotome but avoiding the caudal half. Little is known about the molecular nature of the cues \u2013 intrinsic to the somites \u2013 that are responsible for this segmental migration of neural crest cells.\n\nResults: We demonstrate that Eph-related receptor tyrosine kinases and their ligands are essential for the segmental migration of avian trunk neural crest cells through the somites. EphB3 localizes to the rostral half-sclerotome, including the neural crest, and the ligand ephrin-B1 has a complementary pattern of expression in the caudal half-sclerotome. To test the functional significance of this striking asymmetry, soluble ligand ephrin-B1 was added to interfere with receptor function in either whole trunk explants or neural crest cells cultured on alternating stripes of ephrin-B1 versus fibronection. Neural crest cells in vitro avoided migrating on lanes of immobilized ephrin-B1; the addition of soluble ephrin-B1 blocked this inhibition. Similarly, in whole trunk explants, the metameric pattern of neural crest migration was disrupted by addition of soluble ephrin-B1, allowing entry of neural crest cells into caudal portions of the sclerotome.\n\nConclusions: Both in vivo and in vitro, the addition of soluble ephrin-B1 results in a loss of the metameric migratory pattern and a disorganization of neural crest cell movement. These results demonstrate that Eph-family receptor tyrosine kinases and their transmembrane ligands are involved in interactions between neural crest and sclerotomal cells, mediating an inhibitory activity necessary to constrain neural precursors to specific territories in the developing nervous system.", "date": "1997-08-01", "date_type": "published", "publication": "Current Biology", "volume": "7", "number": "8", "publisher": "Cell Press", "pagerange": "571-580", "id_number": "CaltechAUTHORS:20160323-141712284", "issn": "0960-9822", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-141712284", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "National Institute of Mental Health", "grant_number": "MH-49176" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1016/S0960-9822(06)00256-9", "resource_type": "article", "pub_year": "1997", "author_list": "Krull, Catherine E.; Lansford, Rusty; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8mj53-06120", "eprint_id": 28866, "eprint_status": "archive", "datestamp": "2023-08-22 11:47:30", "lastmod": "2023-10-24 18:12:08", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Saldivar-J-R", "name": { "family": "Saldivar", "given": "Jose R." } }, { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John W." } }, { "id": "Krull-C-E", "name": { "family": "Krull", "given": "Catherine E." } }, { "id": "Ruffins-S-W", "name": { "family": "Ruffins", "given": "Seth" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dorsal hindbrain ablation results in rerouting of neural crest migration and changes in gene expression, but normal hyoid development", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; hindbrain; Hox genes; chicken; cell migration", "note": "\u00a9 1997 The Company of Biologists Limited. \n\nAccepted 1 May 1997. \n\nWe thank Drs Scott Fraser, Mark Selleck and Carole LaBonne for critically reading the manuscript and Simone Lutolf, Katrin Wunnenberg-Stapleton, Roham Zamanian and Parisa Zarbafian for assistance with DiI-labeling, microsurgeries and cryosectioning and Drs Robb Krumlauf and David Wilkinson for providing cDNA probes. This work was supported by NS57315 and HD15527 to M. B. F. and\nminority supplement on DE10066 for J. R. S.\n\nPublished - SALdev97.pdf
", "abstract": "Our previous studies have shown that hindbrain neural\ntube cells can regulate to form neural crest cells for a\nlimited time after neural fold removal (Scherson, T.,\nSerbedzija, G., Fraser, S. E. and Bronner-Fraser, M. (1993).\nDevelopment 188, 1049-1061; Sechrist, J., Nieto, M. A.,\nZamanian, R. T. and Bronner-Fraser, M. (1995). Development\n121, 4103-4115). In the present study, we ablated the\ndorsal hindbrain at later stages to examine possible alterations in migratory behavior and/or gene expression in\nneural crest populations rostral and caudal to the operated\nregion. The results were compared with those obtained by\nmisdirecting neural crest cells via rhombomere rotation.\nFollowing surgical ablation of dorsal r5 and r6 prior to the\n10 somite stage, r4 neural crest cells migrate along normal\npathways toward the second branchial arch. Similarly, r7\nneural crest cells migrate primarily to the fourth branchial\narch. When analogous ablations are performed at the 10-\n12 somite stage, however, a marked increase in the numbers\nof DiI/Hoxa-3-positive cells from r7 are observed within the\nthird branchial arch. In addition, some DiI-labeled r4 cells\nmigrate into the depleted hindbrain region and the third\nbranchial arch. During their migration, a subset of these r4\ncells up-regulate Hoxa-3, a transcript they do not normally\nexpress. Krox20 transcript levels were augmented after\nablation in a population of neural crest cells migrating from r4, caudal r3 and rostral r3. Long-term survivors of\nbilateral ablations possess normal neural crest-derived\ncartilage of the hyoid complex, suggesting that misrouted\nr4 and r7 cells contribute to cranial derivatives appropriate for their new location. In contrast, misdirecting of the neural crest by rostrocaudal rotation of r4 through r6 results in a reduction of Hoxa-3 expression in the third branchial arch and corresponding deficits in third arch-derived structures of the hyoid apparatus. These results demonstrate that neural crest/tube progenitors in the hindbrain can compensate by altering migratory trajectories and patterns of gene expression when the adjacent neural crest is removed, but fail to compensate appropriately when the existing neural crest is misrouted by neural tube rotation.", "date": "1997-07", "date_type": "published", "publication": "Development", "volume": "124", "number": "14", "publisher": "Company of Biologists", "pagerange": "2729-2739", "id_number": "CaltechAUTHORS:20120119-134915476", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120119-134915476", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS-57315" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "DE-10066" } ] }, "primary_object": { "basename": "SALdev97.pdf", "url": "https://authors.library.caltech.edu/records/8mj53-06120/files/SALdev97.pdf" }, "resource_type": "article", "pub_year": "1997", "author_list": "Saldivar, Jose R.; Sechrist, John W.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8xy2b-xz158", "eprint_id": 63297, "eprint_status": "archive", "datestamp": "2023-08-19 01:06:29", "lastmod": "2023-10-25 23:42:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "McClay-D-R", "name": { "family": "McClay", "given": "David R." } }, { "id": "Damsky-C-H", "name": { "family": "Damsky", "given": "Caroline H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Wylie-C", "name": { "family": "Wylie", "given": "Chris" } }, { "id": "Bernfield-M", "name": { "family": "Bernfield", "given": "Merton" } }, { "id": "Markwald-R-R", "name": { "family": "Markwald", "given": "Roger R." } } ] }, "title": "Cellular migration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1997 Elsevier.", "abstract": "[no abstract]", "date": "1997-03", "date_type": "published", "publication": "Reproductive Toxicology", "volume": "11", "number": "2-3", "publisher": "Elsevier", "pagerange": "321-329", "id_number": "CaltechAUTHORS:20160101-185449768", "issn": "0890-6238", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-185449768", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0890-6238(96)00215-8", "resource_type": "article", "pub_year": "1997", "author_list": "McClay, David R.; Damsky, Caroline H.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/k84hc-xtr17", "eprint_id": 63298, "eprint_status": "archive", "datestamp": "2023-08-19 00:48:06", "lastmod": "2024-01-13 16:30:34", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Inductive interactions underlie neural crest formation", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 1998 Academic Press.", "abstract": "The neural crest is a transient population of cells defined in terms of their ectodermal origin, their migratory behavior, and their derivatives. Neural crest cells classically have been thought to be a segregated population within the neural plate, bordered laterally by presumptive epidermis and medially by prospective central nervous system (CNS). Cell lineage analyses in vitro and in vivo have demonstrated that individual neural crest cells are multipotent. In fact, they have the properties of stem cells with at least a limited ability to self-renew. The experiments summarized in this chapter demonstrate that interactions between the presumptive neural plate and the nonneural ectoderm lead to induction of the avian neural crest at the interface between these tissues. This supports the idea that an inductive signal travels through the epidermis to generate neural crest cells in the gastrulating embryo. On the other hand, the competence of the neural plate to form different types of neural crest derivatives appears to change with time. BMP4 and BMP7 (bone morphogenetic proteins) have been shown to be sufficient to substitute for the nonneural ectoderm in inducing neural crest cells. The neural tube has a characteristic polarity along the rostrocaudal and the dorsoventral axes. Rostrocaudal regionalization is manifested by the formation of subdivisions in the neural tube, such as the forebrain, midbrain, hindbrain, and spinal cord. Neural crest cells arising from different axial levels contribute to derivatives characteristic of the axial level of origin. It has been demonstrated that dorsalizing signals influence development of not only the neural crest, but also the dorsal neural tube. Various genes are selectively expressed in the dorsal, but not in the ventral, portion of the neural tube.", "date": "1997", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "San Diego, CA", "pagerange": "883-887", "id_number": "CaltechAUTHORS:20160101-190432495", "isbn": "978-0-12-032943-4", "book_title": "Catacholamines: bridging basic science with clinical medicine", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-190432495", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechAUTHORS", "contributors": { "items": [ { "id": "Goldstein-D-S", "name": { "family": "Goldstein", "given": "David S." } }, { "id": "Einsenhofer-G", "name": { "family": "Eisenhofer", "given": "Graeme" } }, { "id": "McCarty-R", "name": { "family": "McCarty", "given": "Richard" } } ] }, "doi": "10.1016/S1054-3589(08)60888-1", "resource_type": "book_section", "pub_year": "1997", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/epjwc-nna73", "eprint_id": 65585, "eprint_status": "archive", "datestamp": "2023-08-19 00:25:50", "lastmod": "2023-10-18 16:09:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kil-Sung-H", "name": { "family": "Kil", "given": "Sung H." } }, { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Inhibition of Cranial Neural Crest Adhesion in Vitro and Migration in Vivo Using Integrin Antisense Oligonucleotides", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1996 Academic Press, Inc. \n\nReceived 8 May 1996, Accepted 16 July 1996, Available online 7 May 2002.\n\nWe thank Drs. Catherine Krull and Ben Murray for critical reading of the manuscript. This work was supported by USPHS HD-15527 to M.B.-F.", "abstract": "Although it is well-established that \u03b2_1 integrins play a functional role in the migration of cranial neural crest cells, little is known about the number or importance of their associated \u03b1 subunits. Here, we have utilized antisense oligonucleotides (aONs) against various mammalian integrin \u03b1 subunits to functionally \"knock out\" integrinsin vitro and in vivo. First, we examined the attachment in vitro of cranial neural crest cells to fibronectin and laminin in the presence of antisense or reversed-sense oligonucleotides using a quantitative adhesion assay. We found three \u03b1 integrin aONs that blocked attachment to fibronectin substrates only, one that blocked attachment to laminin substrates only, and one that blocked attachment to both fibronectin and laminin. As expected, an aON to chick \u03b2_1 integrin reduced attachment to both fibronectin and laminin substrates. These results suggest that there are three or more functionally distinct integrin heterodimers on avian cranial neural crest cells. Second, we examined the ability of aONs against various \u03b1 integrin subunits to perturb cranial neural crest migrationin vivoby injecting the oligonucleotides into the cranial mesenchyme through which neural crest cells migrate. Those \u03b1 aONs that inhibited cell attachment in vitro also caused neural crest and/or neural tube abnormalities after injection in vivo.In addition, two aONs that had no effect in vitro did affect emigration of neural crest cells in vivo. Immunoprecipitations revealed that some integrin subunits were depleted after treatment with antisense but not reversed-sense oligonucleotides both in vivo and in vitro. The results suggest that integrin \u03b1 subunits are required for cranial neural crest cell attachment and emigration.", "date": "1996-10-10", "date_type": "published", "publication": "Developmental Biology", "volume": "179", "number": "1", "publisher": "Elsevier", "pagerange": "91-101", "id_number": "CaltechAUTHORS:20160322-094126992", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-094126992", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" } ] }, "collection": "CaltechAUTHORS", "doi": "10.1006/dbio.1996.0243", "resource_type": "article", "pub_year": "1996", "author_list": "Kil, Sung H.; Lallier, Thomas; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/teg10-3xv80", "eprint_id": 65925, "eprint_status": "archive", "datestamp": "2023-08-19 00:18:30", "lastmod": "2023-10-18 16:55:50", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The genesis of avian neural crest cells: A classic embryonic induction", "ispublished": "pub", "full_text_status": "public", "keywords": "chicken embryo, neural development, peripheral nervous system, inductive interactions", "note": "\u00a9 1996 National Academy of Sciences. \n\nThis paper was presented at a colloquium entitled \"Biology of Developmental Transcription Control, \" organized by Eric H. Davidson, Roy J. Britten, and Gary Felsenfeld, held October 26-28, 1995, at the National Academy of Sciences in Irvine, CA. \n\nWe are grateful to Drs. Kristin Artinger, Clare Baker, Roberto Mayor, and Catherine Krull for helpful comments on the manuscript. M.A.J.S. is currently supported by a Markey research fellowship. This work was partially supported by U.S. Public Health Service Grants HD25138 and NS34617 to M.B.-F. and by a grant from the March of Dimes Birth Defects Foundation. \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U.S.C. \u00a71734 solely to indicate this fact.\n\nPublished - 9352.pdf
", "abstract": "Neural crest cells arise from the ectoderm and are first recognizable as discrete cells in the chicken embryo when they emerge from the neural tube. Despite the classical view that neural crest precursors are a distinct population lying between epidermis and neuroepithelium, our results demonstrate that they are not a segregated population. Cell lineage analyses have demonstrated that individual precursor cells within the neural folds can give rise to epidermal, neural crest, and neural tube derivatives. Interactions between the neural plate and epidermis can generate neural crest cells, since juxtaposition of these tissues at early stages results in the formation of neural crest cells at the interface. Inductive interactions between the epidermis and neural plate can also result in \"dorsalization\" of the neural plate, as assayed by the expression of the Wnt transcripts characteristic of the dorsal neural tube. The competence of the neural plate changes with time, however, such that interaction of early neural plate with epidermis generates only neural crest cells, whereas interaction of slightly older neural plate with epidermis generates neural crest cells and Wnt-expressing cells. At cranial levels, neuroepithelial cells can regulate to generate neural crest cells when the endogenous neural folds are removed, probably via interaction of the remaining neural tube with the epidermis. Taken together, these experiments demonstrate that: (i) progenitor cells in the neural folds are multipotent, having the ability to form multiple ectodermal derivatives, including epidermal, neural crest, and neural tube cells; (ii) the neural crest is an induced population that arises by interactions between the neural plate and the epidermis; and (iii) the competence of the neural plate to respond to inductive interactions changes as a function of embryonic age.", "date": "1996-09-03", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "93", "number": "18", "publisher": "National Academy of Sciences", "pagerange": "9352-9357", "id_number": "CaltechAUTHORS:20160405-100819036", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160405-100819036", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Lucille P. Markey Charitable Trust" }, { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "NS34617" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "collection": "CaltechAUTHORS", "pmcid": "PMC38431", "primary_object": { "basename": "9352.pdf", "url": "https://authors.library.caltech.edu/records/teg10-3xv80/files/9352.pdf" }, "resource_type": "article", "pub_year": "1996", "author_list": "Selleck, Mark A. J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/4fd5b-tfq08", "eprint_id": 66052, "eprint_status": "archive", "datestamp": "2023-08-22 11:04:15", "lastmod": "2023-10-18 17:05:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kil-Sung-H", "name": { "family": "Kil", "given": "Sung H." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Expression of the Avian \u03b1_7-Integrin in Developing Nervous System and Myotome", "ispublished": "pub", "full_text_status": "restricted", "keywords": "cell surface receptors; extracellular matrix; neural tube; myotome", "note": "\u00a9 1996 Published by Elsevier Science Ltd. Received 11 August 1995; revised 29 October 1995; accepted 29 October 1995. Available online 5 March 1999.\n\nWe thank Patty Wang for excellent technical assistance. This work was supported by USPHS HD-15527 to MBF.", "abstract": "Integrins are cell surface receptors for a variety of extracellular matrix molecules including fibronectin, laminin and collagens. Although their role in development is not completely understood, they are likely to have important functions in cell migration and axon guidance. To characterize the types of integrins expressed in the developing nervous system, we have used monoclonal antibodies against \u03b1_7- and \u03b1_v-integrin subunits to examine the distribution of these subunits in the early chick embryo. Low levels of \u03b1_7 immunoreactivity were first observed in the neural tube and developing myotome of stage 17 embryos (E2.5). Although low levels of \u03b1_7 expression were associated with most neuroepithelial cells, distinct \u03b1_7 immunoreactivity was first detected in the ventrolateral portions of the neural tube at a stage corresponding to the time when the first neurons differentiate. Its distribution pattern overlapped with that of commissural neurons in the developing spinal cord. \u03b1_7 was also prominently localized to the motor neurons and their axons emanating from the neural tube. In addition, \u03b17 immunoreactivity was observed on a subpopulation of trunk neural crest cells migrating through the somitic sclerotome. At later stages, \u03b1_7 expression was observed in other nervous system structures such as the pigmented retinal epithelial cells. In addition to its distribution in the developing nervous system, \u03b1_7 immunoreactivity was associated with early myotomal cells shortly after myotome formation and its expression persisted throughout myotome development. In contrast to \u03b1_7, \u03b1_v-integrin had a limited distribution in the nervous system, being expressed only at low levels in the neural tube. However, \u03b1_v displayed prominent immunoreactivity in the myotome and in endothelial cells of the dorsal aorta. The results suggest that \u03b1_7-integrin is one of the prevalent integrin subunits on neurons and axons in the developing spinal cord.", "date": "1996-06", "date_type": "published", "publication": "International Journal of Developmental Neuroscience", "volume": "14", "number": "3", "publisher": "Elsevier", "pagerange": "181-190", "id_number": "CaltechAUTHORS:20160411-114742463", "issn": "0736-5748", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160411-114742463", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" } ] }, "doi": "10.1016/0736-5748(96)00006-8", "resource_type": "article", "pub_year": "1996", "author_list": "Kil, Sung H. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cp7y3-01309", "eprint_id": 65606, "eprint_status": "archive", "datestamp": "2023-08-18 23:54:43", "lastmod": "2023-10-18 16:10:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Pettway-Unno-Zo\u00e9", "name": { "family": "Pettway", "given": "Zo\u00e9" }, "orcid": "0000-0002-5176-185X" }, { "id": "Domowicz-Miriam", "name": { "family": "Domowicz", "given": "Miriam" }, "orcid": "0000-0001-7860-4427" }, { "id": "Schwartz-Nancy-B", "name": { "family": "Schwartz", "given": "Nancy B." }, "orcid": "0000-0002-7613-0894" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Age-Dependent Inhibition of Neural Crest Migration by the Notochord Correlates with Alterations in the S103L Chondroitin Sulfate Proteoglycan", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1996 Academic Press, Inc. \n\nReceived December 18, 1995. Revised version received March 1, 1996. \n\nWe thank Judith Henry for embryo dissection and Dr. Catherine Krull for helpful comments on the manuscript. This work was supported by AR-19622 and HD-09402 to N.B.S. and HD-15527, DE 10066, and a grant from the Muscular Dystrophy Foundation to M.B.F.", "abstract": "In avian embryos, the notochord inhibits neural crest migration, resulting in the absence of neural crest cells from the perinotochordal space. Here, we test whether temporal changes in the ability of the notochord to inhibit neural crest migration correlate with alterations in the S103L chondroitin sulfate proteoglycan (CSPG). Because CSPGs are abundant in the perinotochordal space and the inhibitory effects of the notochord are chondroitinase sensitive bothin vivoandin vitro,we examined the distribution and biochemical nature of a large CSPG whose core protein is recognized by the S103L antibody. The S103L CSPG is specific to the perinotochordal space during the course of neural crest migration and codistributes with the HNK-1 carbohydrate. Biochemical characterization reveals that the S103L CSPG bears the HNK-1 epitope and is the only HNK-1 immunoreactive proteoglycan present around the notochord at these stages. Following neural crest migration, the S103L CSPG staining is maintained in the perinotochordal region and also is expressed later in cartilage. In 4-day-old embryos, however, the S103L CSPG undergoes a reduction of HNK-1 immunoreactivity. To examine the temporal nature of the notochord's inhibitory ability, we assayed the effects on neural crest migration of grafting notochords from 2- to 5-day-old donor quail embryos into 2-day-old host chick embryos. Donor notochords from 2- to 3-day-old embryos inhibit neural crest cell migration, whereas the degree of inhibition is reduced or absent when notochords are derived from \u22654-day-old donors. This suggests that older notochords lose their inhibitory ability. Interestingly, preincubation of younger notochords with the HNK-1 antibody blocks the inhibitory effect, suggesting that glycosylation of the perinotochordal matrix may be important. The time when the notochord loses its inhibitory ability as assessed by ourin vivografting assay correlates with the biochemical and immunocytochemical changes in the notochordal S103L antigen. These data suggest that a species of S103L CSPG, which is expressed by the early notochord and bears the HNK-1 epitope, may be important for the inhibition of neural crest migration.", "date": "1996-05-25", "date_type": "published", "publication": "Experimental Cell Research", "volume": "225", "number": "1", "publisher": "Elsevier", "pagerange": "195-206", "id_number": "CaltechAUTHORS:20160323-081758861", "issn": "0014-4827", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-081758861", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "AR-19622" }, { "agency": "NIH", "grant_number": "HD-09402" }, { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "NIH", "grant_number": "DE 10066" }, { "agency": "Muscular Dystrophy Foundation" } ] }, "doi": "10.1006/excr.1996.0170", "resource_type": "article", "pub_year": "1996", "author_list": "Pettway, Zo\u00e9; Domowicz, Miriam; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fkykc-48207", "eprint_id": 29204, "eprint_status": "archive", "datestamp": "2023-08-22 10:55:07", "lastmod": "2023-10-24 18:26:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Saldivar-J-R", "name": { "family": "Saldivar", "given": "Jose R." } }, { "id": "Krull-C-E", "name": { "family": "Krull", "given": "Catherine E." } }, { "id": "Krumlauf-R", "name": { "family": "Krumlauf", "given": "Robb" }, "orcid": "0000-0001-9102-7927" }, { "id": "Ariza-McNaughton-L", "name": { "family": "Ariza-McNaughton", "given": "Linda" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Rhombomere of origin determines autonomous versus environmentally regulated expression of Hoxa3 in the avian embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; hindbrain; Hox gene; chick; rhombomere", "note": "\u00a9 1996 Company of Biologists Limited.\n\nAccepted 23 November 1995.\n\nThe first two authors contributed equally to this work. We thank Phoebe Villanueva and Roham Zamanian for excellent technical support and Drs John Sechrist and Scott Fraser for helpful suggestions and critical reading of the manuscript. This work was supported by USPHS grants DE10066 and HD15527 to M. B. F.\n\nPublished - SALdev96.pdf
", "abstract": "We have investigated the pattern and regulation of Hoxa3 expression in the hindbrain and associated neural crest cells in the chick embryo, using whole mount in situ hybridization in conjunction with DiI labeling of neural crest cells and microsurgical manipulations. Hoxa3 is expressed in the neural plate and later in the neural tube with a rostral border of expression corresponding to the boundary between rhombomeres (r) 4 and 5. Initial expression is diffuse and becomes sharp after boundary formation. Hoxa3 exhibits uniform expression within r5 after formation of rhombomeric borders. Cell marking experiments reveal that neural crest cells migrating caudally, but not rostrally, from r5 and caudally from r6 express Hoxa3 in normal embryo. Results from transposition experiments demonstrate that expression of Hoxa3 in r5 neural crest cells is not strictly cell-autonomous. When r5 is transposed with r4 by rostrocaudal rotation of the rhomobomeres, Hoxa3 is expressed in cells migrating lateral to transposed r5 and for a short time, in condensing ganglia, but not by neural crest within the second branchial arch. Since DiI-labeled cells from transposed r5 are present in the second arch, Hoxa3-expressing neural crest cells from r5 appear to down-regulate their Hoxa3 expression in their new environment. In contrast, when r6 is transposed to the position of r4 after boundary formation, Hoxa3 is maintained in both migrating neural crest cells and those positioned within the second branchial arch and associated ganglia. These results suggest that Hoxa3 expression is cell-autonomous in r6 and its associated neural crest. Our results suggest that neural crest cells expressing the same Hox gene are not eqivalent; they respond differently to environmental signals and exhibit distinct degrees of cell autonomy depending upon their rhombomere of origin.", "date": "1996-03-01", "date_type": "published", "publication": "Development", "volume": "122", "number": "3", "publisher": "Company of Biologists", "pagerange": "895-904", "id_number": "CaltechAUTHORS:20120208-115800543", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120208-115800543", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "DE-10066" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" } ] }, "primary_object": { "basename": "SALdev96.pdf", "url": "https://authors.library.caltech.edu/records/fkykc-48207/files/SALdev96.pdf" }, "resource_type": "article", "pub_year": "1996", "author_list": "Saldivar, Jose R.; Krull, Catherine E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/s0eh5-26n08", "eprint_id": 65781, "eprint_status": "archive", "datestamp": "2023-08-20 07:13:57", "lastmod": "2023-10-18 16:47:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-Roberto", "name": { "family": "Perris", "given": "Roberto" }, "orcid": "0000-0001-5626-5233" }, { "id": "Perissinotto-Daniela", "name": { "family": "Perissinotto", "given": "Daniela" } }, { "id": "Pettway-Unno-Zo\u00e9", "name": { "family": "Pettway", "given": "Zo\u00e9" }, "orcid": "0000-0002-5176-185X" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "M\u00f6rgelin-Matthias", "name": { "family": "M\u00f6rgelin", "given": "Matthias" }, "orcid": "0000-0002-6212-6990" }, { "id": "Kimata-Koji", "name": { "family": "Kimata", "given": "Koji" } } ] }, "title": "Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest, cell migration, proteoglycans, aggrecan,\nversican", "note": "\u00a9 1996 The Federation of American Societies for Experimental Biology. \n\nReceived for publication August 7, 1995. Accepted for publication October 17, 1995. \n\nWe are indebted to Mats Paulsson and Michael Sorrell for the various purified matrix molecules and antibodies. The work was supported by grants from Associazione Italians Ricerca sul Cancro, National Institute of Health grant USPHS HD 15527, the M. E. Muller Foundation, the Swedish Medical Research Council, the Swedish Natural Research Council, and the Roche Research Foundation.", "abstract": "Aggrecans and PG-M/versicans represent two newly defined families of hyaluronan-binding proteoglycans for which the function is still poorly understood. Using the avian neural crest as a model system, we have examined the molecular mechanisms entailed in the cell-proteoglycan interaction during embryonic cell motility. Both the primary cartilage aggrecan of the avian embryo (PG-H/aggrecan) and the largest variant of the avian mesenchymal versican (PG-M/versican VO) failed to support neural crest cell adhesion and migration when topographically immobilized onto the substrate. Conversely, solely the PG-H/aggrecan, and similar aggrecans from other species, counteracted the migration-promoting effect of a number of matrix molecules lacking proteoglycan affinity. This inhibitory effect was not reproduced by the isolated glycosaminoglycan chains, the isolated core protein, the reduced and alkylated macromolecule, or the aggrecan in which the G1 hyaluronan-binding domain had been inactivated with hyaluronan fragments or antibodies. Limited depolymerization of the side chains and preincubation of the PG-H/aggrecan with anti-glycosaminoglycan antibodies differentially reduced the inhibitory activity of the proteoglycan on cell motility. The results demonstrate a diverse inhibitory effect of aggrecans and PG-M/versicans on embryonic cell movement and show that the inhibitory action of aggrecans is independent of substrate binding, is dependent on a G1 domain-mediated association of the intact proteoglycan with cell surface-bound hyaluronan, and is differentially mediated by its glycosaminoglycan side chains.", "date": "1996-02", "date_type": "published", "publication": "FASEB Journal", "volume": "10", "number": "2", "publisher": "Federation of American Societies for Experimental Biology", "pagerange": "293-301", "id_number": "CaltechAUTHORS:20160331-081138066", "issn": "0892-6638", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160331-081138066", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Associazione Italians Ricerca sul Cancro" }, { "agency": "NIH", "grant_number": "HD15527" }, { "agency": "M. E. Muller Foundation" }, { "agency": "Swedish Medical Research Council" }, { "agency": "Swedish Natural Research Council" }, { "agency": "Roche Research Foundation" } ] }, "resource_type": "article", "pub_year": "1996", "author_list": "Perris, Roberto; Perissinotto, Daniela; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/6dbsy-qw136", "eprint_id": 65584, "eprint_status": "archive", "datestamp": "2023-08-20 07:09:16", "lastmod": "2023-10-18 16:08:54", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Hayamizu-T-F", "name": { "family": "Hayamizu", "given": "Terry F." } }, { "id": "Ohsugi-K", "name": { "family": "Ohsugi", "given": "Kojune" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Bryant-S-V", "name": { "family": "Bryant", "given": "Susan V." } } ] }, "title": "Digit Induction by Hensen's Node and Notochord Involves the Expression of shh but Not RAR-\u03b22", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1996 Academic Press, Inc. Received 14 June 1995, Accepted 31 October 1995, Available online 7 May 2002.\n\nWe are grateful to Drs. David Gardiner and Mary Dickinson and the anonymous reviewers for helpful comments on the manuscript. We thank Bruce Blumberg and Kaz Umesono for the RAR-\u03b22 probe and Juan Carlos Izpisua-Belmonte for the ssh probe. M.A.J.S. was supported by a Muscular Dystrophy Association research fellowship. Research was supported by HD25138 and a grant from March of Dimes Birth Defects Foundation to M.B.-F and HD25620 to S.V.B.", "abstract": "It is well established that Hensen's nodes can induce the formation of supernumerary digits after grafting into the anterior margin of the developing limb bud. The recent finding that distinct mesodermal cell populations are segregated within the node has made it possible to isolate different prospective cell types in an attempt to correlate digit-inducing ability with cell fate. We find that the prospective notochord cells contained within Hensen's node are able to induce supernumerary digits, whereas presumptive somite cells cannot. This early difference in inducing ability persists into later stages of development: epithelial somites are unable to induce while notochord from all lengths of the neuraxis continues to induce. Using probes to retinoic acid receptor-\u03b22 and sonic hedgehog (shh) we find no evidence to support the idea that inducing tissues generate extra digits by releasing retinoic acid into adjacent limb tissue but find that the inducing ability of a tissue correlates with its expression of shh.", "date": "1996-01-10", "date_type": "published", "publication": "Developmental Biology", "volume": "173", "number": "1", "publisher": "Elsevier", "pagerange": "318-326", "id_number": "CaltechAUTHORS:20160322-094126495", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-094126495", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Association" }, { "agency": "NIH", "grant_number": "HD25138" }, { "agency": "March of Dimes Birth Defects Foundation" }, { "agency": "NIH", "grant_number": "HD25620" } ] }, "doi": "10.1006/dbio.1996.0026", "resource_type": "article", "pub_year": "1996", "author_list": "Selleck, Mark A. J.; Hayamizu, Terry F.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0gm8d-8xb26", "eprint_id": 65267, "eprint_status": "archive", "datestamp": "2023-08-20 06:59:13", "lastmod": "2024-01-13 16:43:48", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Manipulations of Neural Crest Cells or Their Migratory Pathways", "ispublished": "unpub", "full_text_status": "restricted", "note": "\u00a9 1996 Academic Press, Inc. \n\nThe author thanks Drs. Mary Dickinson, Scott Fraser, and Catherine Krull for helpful comments on the manuscript. Development of some of the methods described in this chapter comes from support by NIH Grants HD-25138, HD-15527, and DE-10066.", "abstract": "This chapter discusses the manipulations of neural crest cells or their migratory pathways. The formation of the embryo involves intricate cell movements, cell proliferation, and differentiation. The neural crest has long served as a model for the study of these processes, because neural crest cells undergo extensive migrations and give rise to many diverse derivatives. Neural crest cells arise from the dorsal portion of the neural tube. Several unique properties of these cells make the neural crest an ideal system for studying cell migration and differentiation. First, these cells migrate extensively along characteristic pathways. Second, they give rise to diverse and numerous derivatives, ranging from pigment cells and cranial cartilage to adrenal chromaffin cells and the ganglia of the peripheral nervous system. In addition, their characteristic position of premigratory neural crest cells within the dorsal portion of the neural tube makes them accessible to surgical and molecular manipulations during the initial stages in their development.", "date": "1996", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "San Diego, CA", "pagerange": "61-79", "id_number": "CaltechAUTHORS:20160310-141731239", "isbn": "9780125641531", "book_title": "Methods in Avian Embryology", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160310-141731239", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "NIH", "grant_number": "DE-10066" } ] }, "contributors": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" } } ] }, "doi": "10.1016/S0091-679X(08)60622-6", "resource_type": "book_section", "pub_year": "1996", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t7mkz-18n43", "eprint_id": 65288, "eprint_status": "archive", "datestamp": "2023-09-15 05:19:00", "lastmod": "2023-10-23 21:16:27", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott" }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dorsal and Ventral Cell Types Can Arise from Common Neural Tube Progenitors", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1995 Academic Press, Inc. \n\nReceived for publication 30 June 1995, Accepted 22 August 1995. \n\nWe thank Drs. A. Collazo, M. Dickinson, C. Krull, J. Sechrist, M. Selleck, and J. Shih for helpful comments on the manuscript, Drs. S. Ruffins and M. Selleck for help with image analysis, and B. Metscher and S. Bryant for help with the polyester wax protocol. We gratefully acknowledge the generous gifts of antibodies from a number of colleagues: Drs. H. Tanaka and T. Yamada for the FP-1 antibody, Dr. Virginia Lee for the neurofilament antibody, and Drs. Ulf Eriksson and Malcom Maden for the anti-CRABP antibodies. This work was supported by USPHS HD-25138 and MH-49176.", "abstract": "To challenge the developmental potential of dorsal neural tube cells and test whether single neuroepithelial cells can give rise to the full range of neural tube derivatives, we grafted a notochord lateral to the closing neural folds. This results in juxtaposition of dorsal and ventral cell types, by inducing floor plate cells and motor neurons dorsally. Clonal analysis with the vital dye lysinated rhodamine dextran showed that both \"dorsal\" and \"ventral\" neural tube derivatives can arise from a single precursor. Cells as diverse as sensory ganglion cells, presumptive pigment cells, roof plate cells, motor neurons, and floor plate cells were observed in the same clone. The presence of such diversity within single clones indicates that the responses to dorsal and ventral signals are not mutually exclusive; even in the early neural tube, neuroepithelial cells are not restricted to form only dorsal or ventral neural tube derivatives.", "date": "1995-12", "date_type": "published", "publication": "Developmental Biology", "volume": "172", "number": "2", "publisher": "Elsevier", "pagerange": "591-601", "id_number": "CaltechAUTHORS:20160311-070433737", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160311-070433737", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "National Institute of Mental Health (NIMH)", "grant_number": "MH-49176" } ] }, "doi": "10.1006/dbio.1995.8038", "resource_type": "article", "pub_year": "1995", "author_list": "Artinger, Kristin B.; Fraser, Scott; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1a18a-c1122", "eprint_id": 29191, "eprint_status": "archive", "datestamp": "2023-09-14 19:24:27", "lastmod": "2023-10-23 20:50:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Nieto-M-A", "name": { "family": "Nieto", "given": "M. Angela" } }, { "id": "Zamanian-R-T", "name": { "family": "Zamanian", "given": "Roham T." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Regulative response of the cranial neural tube after neural fold ablation: spatiotemporal nature of neural crest regeneration and up-regulation of Slug", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, neural fold, Slug, cell migration,\nregeneration, chick", "note": "\u00a9 1995 The Company of Biologists Limited.\n\nAccepted 23 August 1995. \n\nWe thank Drs Mary Dickinson, Mark Selleck and Catherine Krull\nfor careful reading of the manuscript and Phoebe Villanueva and\nBrian Rowe for excellent technical assistance. We are grateful to Dr\nTalma Scherson for her input during the early phases of these experiments.\nThis work was supported by HD-25138 to M. B.-F. and grant\nDGICYT-PB92-0045 from the Spanish Ministry of Education to M.\nA. N.\n\nPublished - SECdev95.pdf
", "abstract": "After unilateral ablation of the avian cranial neural folds, the remaining neuroepithelial cells are able to replace the missing neural crest population (Scherson et al., 1993). Here, we characterize the cellular and molecular nature of this regulative response by defining: (1) the time and location of neural crest cell production by the neuroepithelium; (2) rostrocaudal axial differences in the regulative response; and (3) the onset of expression of Slug, a transcription factor present in premigratory and migrating neural crest cells. Using DiI and HNK-1 antibody labeling techniques, we find that neural crest regeneration occurs only after apposition of the remaining neuroepithelium with the epidermis, suggesting that the developmental mechanism underlying regeneration of the neural crest may recapitulate initial generation of the neural crest. The regulative response occurs maximally at the 3\u20135 somite stage, and slowly declines thereafter. Surprisingly, there are profound regional differences in the regenerative ability. Whereas a robust regulation occurs in the caudal midbrain/hindbrain, the caudal forebrain/rostral midbrain regenerates neural crest to a much lesser extent. After neural fold removal in the hindbrain, regenerated neural crest cells migrate in a segmental pattern analogous to that seen in unablated embryos; a decrease in regulative response appears to occur with increasing depth of the ablation. Up-regulation of Slug appears to be an early response after ablation, with Slug transcripts detectable proximal to the ablated region 5\u20138 hours after surgery and prior to emergence of neural crest cells. Both bilateral and unilateral ablations yield substantial numbers of neural crest cells, though the former recover less rapidly and have greater deficits in neural crest-derived structures than the latter. These experiments demonstrate that the regulative ability of the cranial neuroepithelium to form neural crest depends on the time, location and extent of neural fold ablation.", "date": "1995-12", "date_type": "published", "publication": "Development", "volume": "121", "number": "12", "publisher": "Company of Biologists", "pagerange": "4103-4115", "id_number": "CaltechAUTHORS:20120208-092435226", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120208-092435226", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "Spanish Ministry of Education", "grant_number": "DGICYT-PB92-0045" } ] }, "primary_object": { "basename": "SECdev95.pdf", "url": "https://authors.library.caltech.edu/records/1a18a-c1122/files/SECdev95.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Sechrist, John; Nieto, M. Angela; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0wswe-nxc42", "eprint_id": 65583, "eprint_status": "archive", "datestamp": "2023-09-15 05:19:32", "lastmod": "2023-10-23 21:16:36", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Kenny-D", "name": { "family": "Kenny", "given": "Daryn" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } } ] }, "title": "The Receptor Tyrosine Kinase QEK5 mRNA Is Expressed in a Gradient within the Neural Retina and the Tectum", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1995 Published by Elsevier Inc. Received 26 September 1995, Accepted 19 October 1995, Available online 7 May 2002.\n\nWe thank Dr. S. Fraser for a critical reading of this manuscript. Christophe Marcelle is a fellow of the Human Frontier Science Program Organization. This work was supported by HD 15527 and a grant from the Muscular Dystrophy Association.", "abstract": "In the retinotectal system, positional information has long been postulated to take the form of molecular gradients within both the retina and the tectum. Recent reports have implicated Mek4, a member of the Eph (also named class V) family of tyrosine kinase receptors (RTKs), and two ligands, RAGS and ELF-1, in this process. Here, we report the cloning and distribution pattern of QEK5, another member of the Eph family of RTKs, isolated from a quail cDNA library. During retinal differentiation, QEK5 transcripts accumulate in a ventral to dorsal gradient within the retinal neuroepithelium, where its expression becomes restricted to the ganglion and bipolar cell layers. Within the tectum, QEK5 transcripts are detectable in a posterior to anterior gradient in the ventricular layer and newly formed superficial layers. The pattern of QEK5 expression in the retina and tectum is distinct from that of Mek4, suggesting that complex patterns of Eph RTKs and their ligands may play a role in cell\u2013cell interactions involved in retinotectal projections and differentiation of the central nervous system.", "date": "1995-12", "date_type": "published", "publication": "Developmental Biology", "volume": "172", "number": "2", "publisher": "Elsevier", "pagerange": "708-716", "id_number": "CaltechAUTHORS:20160322-094126074", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-094126074", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD15527" }, { "agency": "Muscular Dystrophy Association" } ] }, "doi": "10.1006/dbio.1995.8083", "resource_type": "article", "pub_year": "1995", "author_list": "Kenny, Daryn; Bronner-Fraser, Marianne; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/z7zak-sxs80", "eprint_id": 65628, "eprint_status": "archive", "datestamp": "2023-08-20 06:28:37", "lastmod": "2023-10-18 16:12:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Hepatocyte growth factor/scatter factor (HGF/SF) in early development: evidence for a role in neural induction", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1995 Published by Elsevier Ltd. Available online 14 August 2000.", "abstract": "Since the pioneering work of\nSpemann and Mangold, who found\nthat transplantation of the dorsal lip\nof the frog blastopore produces a\nsecondary neural axis, embryologists\nhave been intrigued by the process of\nneural induction. However, the molecular\nnature of the inducer(s) has\nremained obscure, largely due to the\ncomplication that neural induction\noften occurs as a secondary consequence\nof mesodermal induction. In\nthe past few years, candidate molecules\nhave been identified that are\nable to induce neural tissue in the\nabsence of mesodenn in the frog.\nThese include noggin and follistatin\n(reviewed in Ref. 1), which are\nthought to function by inhibiting\nactivin. In the chick, recent experiments\nhave implicated hepatocyte\ngrowth factor/scatter factor (HGF/SF)\nas a neural inducer based on it5\nexpression in the primitive streak and\nHensen's node of the early chick\nembryo, and its ability to induce\nneural tissue in naive epiblast. This\nreview describes the evidence supporting\nthe possible function of HGF/SF as an early embryonic inducer.", "date": "1995-11", "date_type": "published", "publication": "Trends in Genetics", "volume": "11", "number": "11", "publisher": "Elsevier", "pagerange": "423-425", "id_number": "CaltechAUTHORS:20160323-134516599", "issn": "0168-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160323-134516599", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/S0168-9525(00)89136-2", "resource_type": "article", "pub_year": "1995", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/9hd1q-apc29", "eprint_id": 29297, "eprint_status": "archive", "datestamp": "2023-08-20 06:26:54", "lastmod": "2023-10-24 22:04:49", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krull-C-E", "name": { "family": "Krull", "given": "Catherine E." } }, { "id": "Collazo-A", "name": { "family": "Collazo", "given": "Andres" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules", "ispublished": "pub", "full_text_status": "public", "keywords": "somite, explant culture, videomicroscopy, avian, neural crest, PNA", "note": "\u00a9 1995 The Company of Biologists Limited. \n\nAccepted 14 August 1995. \n\nWe dedicate this work to the memory of Dr Edmund Arbas. His\ngreat enthusiasm for his family, friends and science will be remembered. We thank Roham Zamanian and Jon Neri for excellent\ntechnical assistance. This work was supported by NIH/NRSA 09459\nto C. E. K., by USPHS 15527 and a Muscular Dystrophy Association\ngrant to M. B.-F., by a NIMH Silvio Conte Center grant to S. E. F.\nand A. C., and Beckman Institute Biological Imaging Center.\n\nPublished - KRUdev95.pdf
", "abstract": "Trunk neural crest cells migrate through the somites in a striking segmental fashion, entering the rostral but not caudal sclerotome, via cues intrinsic to the somites. Attempts to define the molecular bases of these cues have been hampered by the lack of an accessible assay system. To examine trunk neural crest migration over time and to perturb candidate guiding molecules, we have developed a novel explant preparation. Here, we demonstrate that trunk regions of the chicken embryo, placed in explant culture, continue to develop apparently normally for 2 days. Neural crest cells, recognized by prelabeling with DiI or by poststaining with the HNK-1 antibody, migrate in the somites of the explants in their typical segmental pattern. Furthermore, this paradigm allows us to follow trunk neural crest migration in situ for the first time using low-light-level videomicroscopy. The trajectories of individual neural crest cells were often complex, with cells migrating in an episodic mode encompassing forward, backward and lateral movements. Frequently, neural crest cells migrated in close-knit groups of 2\u20134 cells, moving at mean rates of migration of 10\u201314 \u00b5m/hour. Treatment of the explants with the lectin peanut agglutinin (PNA) both slowed the rate and altered the pattern of neural crest migration. Neural crest cells entered both the rostral and caudal halves of the sclerotome with mean rates of migration ranging from 6 to 13 \u00b5m/hour. These results suggest that peanut agglutinin-binding molecules are required for the segmental patterning of trunk neural crest migration. Because this approach permits neural crest migration to be both observed and perturbed, it offers the promise of more direct assays of the factors that influence neural crest development.", "date": "1995-11", "date_type": "published", "publication": "Development", "volume": "121", "number": "11", "publisher": "Company of Biologists", "pagerange": "3733-3743", "id_number": "CaltechAUTHORS:20120215-092512792", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120215-092512792", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH/NRSA", "grant_number": "09459" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "National Institute of Mental Health (NIMH)" }, { "agency": "Caltech Beckman Institute" } ] }, "primary_object": { "basename": "KRUdev95.pdf", "url": "https://authors.library.caltech.edu/records/9hd1q-apc29/files/KRUdev95.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Krull, Catherine E.; Collazo, Andres; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/n96fz-hwy76", "eprint_id": 65408, "eprint_status": "archive", "datestamp": "2023-08-20 06:28:30", "lastmod": "2023-10-18 15:59:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Marcelle-C", "name": { "family": "Marcelle", "given": "Christophe" } }, { "id": "Wolf-J-J", "name": { "family": "Wolf", "given": "John" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The in Vivo Expression of the FGF Receptor FREK mRNA in Avian Myoblasts Suggests a Role in Muscle Growth and Differentiation", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1995 Academic Press, Inc. \n\nAccepted June 14, 1995. \n\nWe thank Dr. Charles Ordahl for discussion and helpful commments on the manuscript. We thank Dr. K. Tosney for insighful discussions during the course of this study. Chritophe Marcelle is a fellow of the Human Frontier Science Program Organization. This work was supported by HD 15527 and a grant from Muscular Dystrophy Association.", "abstract": "Muscle growth during embryogenesis is the result of a balance between the proliferation of myoblasts and their differentiation into mature, contractile fibers. Fibroblast growth factors (FGFs) are potent stimulators of myoblast proliferation and inhibitors of myoblast differentiationin vitro.However, it is not clear at present if FGFs and their receptors regulate this processin vivo,partially because no FGF receptor was known to be expressed by myoblasts during embryogenesis. In this study, we have used quail/chick grafting and BrdU labeling techniques to demonstrate that a recently cloned avian FGF receptor, FREK, is expressed by replicating skeletal muscle myoblasts, while differentiated muscle cells no longer express this receptor. In the limb, muscle progenitors originating from the somite start expressing FREK at 3 days of development (E3). FREK expression in the limb myoblasts follows that of Pax-3 and Pax-7, but precedes that of MyoD. Since MyoD expression signals the onset of terminal differentiation, this demonstrates that FREK is expressed in muscle progenitors prior to overt muscle differentiation. A more complex situation is observed in the trunk region, where a first wave of MyoD-positive myocytes, which are postmitotic and never express FREK, appear in the early myotomal compartment of the somite. Slightly later, at E2.5, FREK-positive myoblasts migrate into the myotome as a second wave of muscle progenitors, 15 hr after the first MyoD-positive cells. FREK's expression by myoblasts arising at all stages of myogenesis indicates that this growth factor receptor represents one of the earliest molecular markers for this cell population. FREK's prominent expression during muscle differentiation sets it apart from other FGF receptors and suggests that this molecule plays an important role during muscle growth and differentiation.", "date": "1995-11", "date_type": "published", "publication": "Developmental Biology", "volume": "172", "number": "1", "publisher": "Elsevier", "pagerange": "100-114", "id_number": "CaltechAUTHORS:20160317-074417281", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160317-074417281", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" } ] }, "doi": "10.1006/dbio.1995.0008", "resource_type": "article", "pub_year": "1995", "author_list": "Marcelle, Christophe; Wolf, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rf9s3-5vz41", "eprint_id": 29310, "eprint_status": "archive", "datestamp": "2023-08-20 06:02:27", "lastmod": "2023-10-24 22:05:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Dickinson-Mary-E", "name": { "family": "Dickinson", "given": "Mary E." } }, { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "McMahon-A-P", "name": { "family": "McMahon", "given": "Andrew P." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Dorsalization of the neural tube by the non-neural ectoderm", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, spinal cord, Wnt genes, pattern formation,\ninduction, cell signaling, chick embryo", "note": "\u00a9 1995 The Company of Biologists Limited.\n\nAccepted 8 April 1995.\nWe would like to thank Angela Nieto for generously providing the\nSlug probe and helpful comments, Kristin Artinger for help with\ncollagen gel culture techniques, Margaret Baron, Laura Burrus,\nH\u00e9l\u00e8ne Dassule, Brigid Hogan and Karen Symes for critical reading\nof the manuscript, Olivia Kelly for stimulating discussions and the\nanonymous reviewers for improving the manuscript. This work was\nsupported by grants to A. P. M. and M. B. F. (USPHS HD-25138)\nfrom NIH. M. E. D. is a graduate fellow supported jointly by\nColumbia University and the Roche Institute of Molecular Biology.\nM. A. J. S. is supported by a fellowship from the Muscular Dystrophy\nAssociation.\n\nPublished - DICdev95.pdf
", "abstract": "The patterning of cell types along the dorsoventral axis of the spinal cord requires a complex set of inductive signals. While the chordamesoderm is a well-known source of ventralizing signals, relatively little is known about the cues that induce dorsal cell types, including neural crest. Here, we demonstrate that juxtaposition of the non-neural and neural ectoderm is sufficient to induce the expression of dorsal markers, Wnt-1, Wnt-3a and Slug, as well as the formation of neural crest cells. In addition, the competence of neural plate to express Wnt-1 and Wnt-3a appears to be stage dependent, occurring only when neural tissue is taken from stage 8\u201310 embryos but not from stage 4 embryos, regardless of the age of the non-neural ectoderm. In contrast to the induction of Wnt gene expression, neural crest cell formation and Slug expression can be induced when either stage 4 or stage 8\u201310 neural plates are placed in contact with the non-neural ectoderm. These data suggest that the non-neural ectoderm provides a signal (or signals) that specifies dorsal cell types within the neural tube, and that the response is dependent on the competence of the neural tissue.", "date": "1995-07", "date_type": "published", "publication": "Development", "volume": "121", "number": "7", "publisher": "Company of Biologists", "pagerange": "2099-2106", "id_number": "CaltechAUTHORS:20120215-140005907", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120215-140005907", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "Columbia University" }, { "agency": "Roche Institute of Molecular Biology" }, { "agency": "Muscular Dystrophy Association" } ] }, "primary_object": { "basename": "DICdev95.pdf", "url": "https://authors.library.caltech.edu/records/rf9s3-5vz41/files/DICdev95.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Dickinson, Mary E.; Selleck, Mark A. J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bctat-meb13", "eprint_id": 65596, "eprint_status": "archive", "datestamp": "2023-08-20 05:57:36", "lastmod": "2023-10-18 16:09:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Origins and Developmental Potential of the Neural Crest", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1995 by Academic Press, Inc. Received December 22, 1994.\nRevised version received February 21, 1995.\n\nOur work is supported by USPHS HD-15527, HD-25138, DE10066,\nand a grant from the Muscular Dystrophy Foundation.", "abstract": "Neural crest cells are a migratory population that forms most of the peripheral nervous system, facial skeleton, and numerous other derivatives. These cells arise from the neural ectoderm and are first recognizable as discrete cells after neural tube closure. In this review, I summarize the results of studies from our laboratory on neural crest cell lineage and origin. Our recent experiments demonstrate that interactions between the presumptive neural plate and the nonneural ectoderm are likely to be instrumental in the induction of the avian neural crest. Juxtaposition of these tissues at early stages results in the formation of neural crest cells at the interface. However, neural crest cells do not appear to be segregated from other neuroepithelial cells; cell lineage studies have demonstrated that individual precursor cells within the neural tube can give rise to both neural crest and neural tube derivatives as diverse as sensory, commissural, and motor neurons. This suggests that individual neuroectodermal cells are multipotent, such that a precursor within the neural tube has the ability to form both neural tube (central nervous system) and neural crest (peripheral nervous system and other) derivatives. Further support for flexibility in the developmental program of neuroepithelial cells comes from experiments in which the cranial neural folds are ablated; this results in regulation by the remaining ventral neural tube cells to form neural crest cells after the endogenous neural crest is removed. At later stage of development, this regulative capacity is lost. Following their emigration from the neural tube, neural crest cells become progressively restricted to defined embryonic states. Taken together, these experiments demonstrate that: (1) the neural crest is an induced population that arises by interactions within the ectoderm; (2) initially, progenitor cells are multipotent, having the potential to form multiple neural crest and neural tube derivatives; and (3) with time, the precursors become progressively restricted to form neural crest derivatives and eventually to individual phenotypes.", "date": "1995-06", "date_type": "published", "publication": "Experimental Cell Research", "volume": "218", "number": "2", "publisher": "Elsevier", "pagerange": "405-417", "id_number": "CaltechAUTHORS:20160322-123820571", "issn": "0014-4827", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-123820571", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-25138" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "DE10066" }, { "agency": "Muscular Dystrophy Foundation" } ] }, "doi": "10.1006/excr.1995.1173", "resource_type": "article", "pub_year": "1995", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rxaq5-10p35", "eprint_id": 65994, "eprint_status": "archive", "datestamp": "2023-08-22 10:24:32", "lastmod": "2023-10-18 17:02:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Nieto-M-A", "name": { "family": "Nieto", "given": "M. Angela" } }, { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Wilkinson-D-G", "name": { "family": "Wilkinson", "given": "David G." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Relationship between spatially restricted Krox-20 gene expression in branchial neural crest and segmentation in the chick embryo hindbrain", "ispublished": "pub", "full_text_status": "public", "keywords": "branchial arches; hindbrain segmentation; Hox code; Krox-20; neural crest", "note": "\u00a9 1995 Oxford University Press. \n\nCommunicated by R.Krumlauf. Received on October 11, 1994; revised on December 19, 1994. \n\nWe thank Brad Martinsen for excellent technical assistance with fixation of embryos and photoconversion of the DiI, Simone Lutolf for help with the cryostat sections and some DiI injections, and Mark Selleck and Kristin Artinger for help with image processing. We are also grateful to Claudio Stern for improvements to the in situ hybridization protocol and advice regarding DiI photoconversion, and to Robb Krumlauf and Scott Fraser for discussions and critical reading of the manuscript. This work was supported by USPHS HD-25138 to M.B.-F., by the Medical Research Council (D.G.W.), and by a grant from the Spanish Ministry of Education (DGICYT-PB92-0045) to M.A.N.\n\nPublished - emboj00032-0127.pdf
", "abstract": "Previous studies have suggested that the rostrocaudal patterning of branchial arches in the vertebrate embryo derives from a coordinate segmental specification of gene expression in rhombomeres (r) and neural crest. However, expression of the Krox-20 gene is restricted to neural crest cells migrating to the third branchial arch, apparently from r5, whereas this rhombomere contributes cells to both the second and third arches. We examined in the chick embryo how this spatially restricted expression is established. Expression occurs in precursors in both r5 and r6, and we show by cell labelling that both rhombomeres contribute to Krox-20-expressing neural crest, emigration occurring first from r6 and later caudally from r5. Krox-20 transcripts are not detected in some precursors in rostral r5, presaging the lack of expression in cells migrating rostrally from this rhombomere. After transposition of r6 to the position of r4 or r5, many Krox-20-expressing cells migrate rostral to the otic vesicle, whereas when r5 is transplanted to the position of r4, only a small number of migrating cells express Krox-20. These results indicate that, in the chick, Krox-20 expression in branchial neural crest does not correlate with rhombomeric segmentation, and that there may be intrinsic differences in regulation between the r5 and r6 Krox-20-expressing populations.", "date": "1995-04-18", "date_type": "published", "publication": "EMBO Journal", "volume": "14", "number": "8", "publisher": "European Molecular Biology Organization", "pagerange": "1697-1710", "id_number": "CaltechAUTHORS:20160407-115541043", "issn": "0261-4189", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160407-115541043", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-25138" }, { "agency": "Medical Research Council (UK)" }, { "agency": "Ministerio de Educaci\u00f3n y Ciencia (MEC)", "grant_number": "DGICYT-PB92-0045" } ] }, "pmcid": "PMC398263", "primary_object": { "basename": "emboj00032-0127.pdf", "url": "https://authors.library.caltech.edu/records/rxaq5-10p35/files/emboj00032-0127.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Nieto, M. Angela; Sechrist, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/dzc6c-g6528", "eprint_id": 29188, "eprint_status": "archive", "datestamp": "2023-08-20 05:39:09", "lastmod": "2023-10-24 18:25:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Birgbauer-E", "name": { "family": "Birgbauer", "given": "Eric" } }, { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott" }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Rhombomeric origin and rostrocaudal reassortment of neural crest cells revealed by intravital microscopy", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, cell migration, rhombomere, intravital\nmicroscopy, chick", "note": "\u00a9 1995 The Company of Biologists Limited.\n\nAccepted 19 December 1994. \n\nWe would like to thank Parisa Zarfabian for her excellent technical\nassistance with the cryostat sectioning. E. B. is a Fellow of The Jane\nCoffin Childs Memorial Fund for Medical Research. This investigation\nhas been aided by a grant from The Jane Coffin Childs Memorial\nFund for Medical Research and the NIH (HD-26864 to S. E. F.; DE-10006 and HD-15527 to M. B.-F.).\n\nPublished - BIRdev95.pdf
", "abstract": "Neural crest cell migration in the hindbrain is segmental, with prominent streams of migrating cells adjacent to rhombomeres (r) r2, r4 and r6, but not r3 or r5. This migratory pattern cannot be explained by the failure of r3 and r5 to produce neural crest, since focal injections of the lipophilic dye, DiI, into the neural folds clearly demonstrate that all rhombomeres produce neural crest cells. Here, we examine the dynamics of hindbrain neural crest cell emigration and movement by iontophoretically injecting DiI into small numbers of cells. The intensely labeled cells and their progeny were repeatedly imaged using low-light-level epifluorescence microscopy, permitting their movement to be followed in living embryos over time. These intravital images definitively show that neural crest cells move both rostrally and caudally from r3 and r5 to emerge as a part of the streams adjacent to r2, r4, and/or r6. Within the first few hours, cells labeled in r3 move within and/or along the dorsal neural tube surface, either rostrally toward the r2/3 border or caudally toward the r3/4 border. The labeled cells exit the surface of the neural tube near these borders and migrate toward the first or second branchial arches several hours after initial labeling. Focal DiI injections into r5 resulted in neural crest cell contributions to both the second and third branchial arches, again via rostrocaudal movements of the cells before migration into the periphery. These results demonstrate conclusively that all rhombomeres give rise to neural crest cells, and that rostrocaudal rearrangement of the cells contributes to the segmental migration of neural crest cells adjacent to r2, r4, and r6. Furthermore, it appears that there are consistent exit points of neural crest cell emigration; for example, cells arising from r3 emigrate almost exclusively from the rostral or caudal borders of that rhombomere.", "date": "1995-04", "date_type": "published", "publication": "Development", "volume": "121", "number": "4", "publisher": "Company of Biologists", "pagerange": "935-945", "id_number": "CaltechAUTHORS:20120208-085812222", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120208-085812222", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Jane Coffin Childs Memorial Fund for Medical Research" }, { "agency": "NIH", "grant_number": "HD-26864" }, { "agency": "NIH", "grant_number": "DE-10006" }, { "agency": "NIH", "grant_number": "HD-15527" } ] }, "primary_object": { "basename": "BIRdev95.pdf", "url": "https://authors.library.caltech.edu/records/dzc6c-g6528/files/BIRdev95.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Birgbauer, Eric; Sechrist, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/e3rd2-p0w28", "eprint_id": 29197, "eprint_status": "archive", "datestamp": "2023-08-20 05:30:14", "lastmod": "2023-10-24 18:25:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Origins of the avian neural crest: the role of neural plate-epidermal interactions", "ispublished": "pub", "full_text_status": "public", "keywords": "cell lineage, chick embryo, ectoderm, induction, neural tube, neurulation, peripheral nervous system, quail embryo", "note": "\u00a9 1995 The Company of Biologists Limited. \n\nAccepted 29 October 1994. \n\nWe are grateful to Scott Fraser, Mary Dickinson, Talma Scherson, Jack Sechrist and Kristin Artinger for helpful comments on the manuscript.\nWe thank Sheila Kristy and Scott Pauli for their assistance in preparing the manuscript. M. A. J. S. is supported by a Muscular Dystrophy Association research fellowship. This work was supported by US PHS HD-25138 to M. B. F.\n\nPublished - SELdev95.pdf
", "abstract": "We have investigated the lineage and tissue interactions that result in avian neural crest cell formation from the ectoderm. Presumptive neural plate was grafted adjacent to non-neural ectoderm in whole embryo culture to examine the role of tissue interactions in ontogeny of the neural crest. Our results show that juxtaposition of non-neural ectoderm and presumptive neural plate induces the formation of neural crest cells. Quail/chick recombinations demonstrate that both the prospective neural plate and the prospective epidermis can contribute to the neural crest. When similar neural plate/epidermal confrontations are performed in tissue culture to look at the formation of neural crest derivatives, juxtaposition of epidermis with either early (stages 4\u20135) or later (stages 6\u201310) neural plate results in the generation of both melanocytes and sympathoadrenal cells. Interestingly, neural plates isolated from early stages form no neural crest cells, whereas those isolated later give rise to melanocytes but not crest-derived sympathoadrenal cells. Single cell lineage analysis was performed to determine the time at which the neural crest lineage diverges from the epidermal lineage and to elucidate the timing of neural plate/epidermis interactions during normal development. Our results from stage 8 to 10+ embryos show that the neural plate/neural crest lineage segregates from the epidermis around the time of neural tube closure, suggesting that neural induction is still underway at open neural plate stages.", "date": "1995-02-01", "date_type": "published", "publication": "Development", "volume": "121", "number": "2", "publisher": "Company of Biologists", "pagerange": "525-538", "id_number": "CaltechAUTHORS:20120208-110012393", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120208-110012393", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Association" }, { "agency": "NIH", "grant_number": "HD-25138" } ] }, "primary_object": { "basename": "SELdev95.pdf", "url": "https://authors.library.caltech.edu/records/e3rd2-p0w28/files/SELdev95.pdf" }, "resource_type": "article", "pub_year": "1995", "author_list": "Selleck, Mark A. J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/72sdc-mfe47", "eprint_id": 63296, "eprint_status": "archive", "datestamp": "2023-08-22 10:12:17", "lastmod": "2023-10-25 23:42:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Origin of the avian neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "Cell lineage; Stem cells; Differentiation; Peripheral nervous system", "note": "\u00a9 1995 AlphaMed Press.", "abstract": "Neural crest cells are derived from a population of multipotent stem cells within the neural tube. They emerge shortly after neural tube closure, migrate extensively in the embryo and localize in numerous sites, where they differentiate into neurons and glia of the peripheral nervous system, cartilage and bone of the face, melanocytes and various other cell types. This review summarizes recent experiments from our laboratory delineating the origin and lineage of avian neural crest cells. Neural crest cells arise from the ectoderm, which also gives rise to presumptive epidermal, placodal and neural tube cells. Fate mapping experiments have demonstrated that the neural crest arises at the juncture between presumptive epidermis and the neural plate. Inductive interactions between these two early tissues can generate neural crest cells, suggesting that signals travel through the epidermis to generate neural crest cells prior to neural tube closure. Injection of lineage tracer into individual cells reveals that a single neural fold can form all ectodermal derivatives (i.e., epidermis, neural tube, neural crest). Even after neural tube closure, neuroepithelial cells have the capacity to form multiple neural crest and neural tube derivatives, including both dorsal and ventral phenotypes, suggesting that neural tube and neural crest cells share a common precursor. Further evidence that neural crest and neural tube cells are intimately related comes from experiments in which the cranial neural folds are ablated. The remaining neural tube cells have the capacity to regulate, at least for a limited time, to compensate for missing neural crest cells. These experiments suggest that the early neuroepithelium has no clear segregation with respect to the neural tube or neural crest. With time, dorsalizing and ventralizing signals may cause neural tube cells to acquire specific cell fates.", "date": "1995", "date_type": "published", "publication": "Stem Cells", "volume": "13", "number": "6", "publisher": "AlphaMed Press", "pagerange": "640-646", "id_number": "CaltechAUTHORS:20160101-184319386", "issn": "1549-4918", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-184319386", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/stem.5530130608", "resource_type": "article", "pub_year": "1995", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/h32qw-jr714", "eprint_id": 63295, "eprint_status": "archive", "datestamp": "2023-08-20 05:09:03", "lastmod": "2023-10-25 23:42:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Patterning of the vertebrate neural crest", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1995 Gordon & Breach.", "abstract": "The mechanisms underlying neural crest cell migration are beginning to be understood thanks to the ability to combine a number of techniques in experimental embryology, cell and molecular biology. In the trunk, cell-cell interactions may predominate, so that the mesodermal somites control the rostrocaudal patterning of neural crest cells and the notochord prevents neural crest cells from crossing the midline. In the hindbrain, the segmental migration of neural crest cells may be influenced both by information inherent to the rhombomeres coupled with environmental signals from neighboring tissues, such as the otic vesicle. There is clearly an intimate relationship between migrating neural crest cells, the neural tube from which they emerge, and tissues through which they move. All of these elements are integral in the control of neural crest migration.", "date": "1995", "date_type": "published", "publication": "Perspectives on developmental neurobiology", "volume": "3", "number": "1", "publisher": "Gordon and Breach", "pagerange": "53-62", "id_number": "CaltechAUTHORS:20160101-183846586", "issn": "1064-0517", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-183846586", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE-10066" }, { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "HD-15527" } ] }, "resource_type": "article", "pub_year": "1995", "author_list": "Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wqc6j-by839", "eprint_id": 65920, "eprint_status": "archive", "datestamp": "2023-08-20 04:54:18", "lastmod": "2023-10-18 16:55:39", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Atchley-W-R", "name": { "family": "Atchley", "given": "William R." } }, { "id": "Fitch-W-M", "name": { "family": "Fitch", "given": "Walter M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular evolution of the MyoD family of transcription factors", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1994 National Academy of Sciences. \n\nContributed by Walter M. Fitch, August 17, 1994. \n\nWe thank Drs. Charles Ordahl, Brian Williams, Susan Bryant, Eric Olson, and Michael Miyamoto for helpful comments on the manuscript and Helene Van for technical assistance. One of us (W.R.A.) is supported by the Alfred P. Sloan Foundation and by grants from the National Institutes of Health (GM-45344) and the National Science Foundation (BSR-910718). W.M.F. is supported by a grant from the National Science Foundation (DEB9096152). M.B.-F. was\nsupported by Muscular Dystrophy Association and by grants from the National Institutes of Health (HD-15527, HD-25138, and DE-10066).\n\nPublished - 11522.pdf
", "abstract": "Myogenesis in skeletal muscle is a cascade of developmental events whose initiation involves the MyoD family of transcription factors. Evolutionary analyses of amino acid sequences of this family of transcriptional activators suggest that the vertebrate genes MyoD1, myf-5, Myog (myogenin), and myf-6 were derived by gene duplications from a single ancestral gene. A common genetic origin predicts some functional redundancy between MyoD1 and myf-5 and between Myog and myf-6. Experimental studies have suggested that these pairs of genes can substitute for each other during myogenesis. Separate analyses of the conserved basic helix-loop-helix and nonconserved flanking elements yield similar branching sequences but show evolutionary change in the basic helix-loop-helix region has occurred at a much slower rate.", "date": "1994-11-22", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "91", "number": "24", "publisher": "National Academy of Sciences", "pagerange": "11522-11526", "id_number": "CaltechAUTHORS:20160405-094246992", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160405-094246992", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Alfred P. Sloan Foundation" }, { "agency": "NIH", "grant_number": "GM-45344" }, { "agency": "NSF", "grant_number": "BSR-910718" }, { "agency": "NSF", "grant_number": "DEB9096152" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "DE-10066" } ] }, "pmcid": "PMC45263", "primary_object": { "basename": "11522.pdf", "url": "https://authors.library.caltech.edu/records/wqc6j-by839/files/11522.pdf" }, "resource_type": "article", "pub_year": "1994", "author_list": "Atchley, William R.; Fitch, Walter M.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/etm6g-zb738", "eprint_id": 29398, "eprint_status": "archive", "datestamp": "2023-08-20 04:23:54", "lastmod": "2023-10-24 22:09:38", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George N." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Developmental potential of trunk neural crest cells in the mouse", "ispublished": "pub", "full_text_status": "public", "keywords": "developmental potential, cell fate, neuron, microinjection", "note": "\u00a9 1994 The Company of Biologists Limited.\n\nAccepted 14 April 1994. \n\nWe thank Andres Collazo for helpful comments on the manuscript\nand Mary Flowers for excellent technical assistance. This work was\nsupported by USPHS HD-26864 to S. E. F. and HD-25138 to M. B.-F.\n\nPublished - SERdev94.pdf
", "abstract": "The availability of naturally occurring and engineered mutations in mice which affect the neural crest makes the mouse embryo an important experimental system for studying neural crest cell differentiation. Here, we determine the normal developmental potential of neural crest cells by performing in situ cell lineage analysis in the mouse by microinjecting lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells in the trunk. Labeled progeny derived from single cells were found in the neural tube, dorsal root ganglia, sympathoadrenal derivatives, presumptive Schwann cells and/or pigment cells. Most embryos contained labeled cells both in the neural tube and at least one neural crest derivative, and numerous clones contributed to multiple neural crest derivatives. The time of injection influenced the derivatives populated by the labeled cells. Injections at early stages of migration yielded labeled progeny in both dorsal and ventral neural crest derivatives, whereas those performed at later stages had labeled cells only in more dorsal neural crest derivatives, such as dorsal root ganglion and presumptive pigment cells. The results suggest that in the mouse embryo: (1) there is a common precursor for neural crest and neural tube cells; (2) some neural crest cells are multipotent; and (3) the timing of emigration influences the range of possible neural crest derivatives.", "date": "1994-07", "date_type": "published", "publication": "Development", "volume": "120", "number": "7", "publisher": "Company of Biologists", "pagerange": "1709-1718", "id_number": "CaltechAUTHORS:20120221-151101703", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120221-151101703", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-26864" }, { "agency": "NIH", "grant_number": "HD-25138" } ] }, "primary_object": { "basename": "SERdev94.pdf", "url": "https://authors.library.caltech.edu/records/etm6g-zb738/files/SERdev94.pdf" }, "resource_type": "article", "pub_year": "1994", "author_list": "Serbedzija, George N.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8d2cz-6ga44", "eprint_id": 65802, "eprint_status": "archive", "datestamp": "2023-08-20 04:25:29", "lastmod": "2023-10-18 16:48:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest cell formation and migration in the developing embryo", "ispublished": "pub", "full_text_status": "restricted", "keywords": "dye labeling, cell movement, extracellular matrix, hindbrain, lineage", "note": "\u00a9 1994 FASEB. \n\nOur work is supported by U.S. Public Health Service HD-15527,\nHD-25138, DE10066, and by a grant from the Muscular Dystrophy\nFoundation.", "abstract": "Neural crest cells arise from the neural tube shortly after its closure and migrate extensively through prescribed regions of the embryos, where they differentiate into most of the peripheral nervous system as well as the facial skeleton and pigment cells. Along the embryonic axis, several distinct neural crest populations differ both in their migratory pathways and range of derivatives. Whereas those cells arising from the midbrain migrate as a uniform sheet of cells, neural crest cells emerging from the hindbrain and trunk regions migrate in a segmented manner. For example, trunk neural crest cells move preferentially through the rostral, but not caudal, half of each somite. Interactions with tissues encountered during migration strongly influence this segmental migratory pattern. For example, the mesodermal somites dictate the segmental migration of trunk neural crest cells and the otic placode appears to attract hindbrain neural crest cells. Although little is known about the molecular basis underlying migration, patterns of gene expression in the hindbrain are thought to contribute to the segmental arrangement of neural crest cells. Furthermore, neural crest cells possess integrin receptors that may be important for interacting with extracellular matrix molecules in their surroundings.", "date": "1994-07", "date_type": "published", "publication": "FASEB Journal", "volume": "8", "number": "10", "publisher": "Federation of American Societies for Experimental Biology", "pagerange": "699-706", "id_number": "CaltechAUTHORS:20160331-104653234", "issn": "0892-6638", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160331-104653234", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "DE10066" }, { "agency": "Muscular Dystrophy Foundation" } ] }, "resource_type": "article", "pub_year": "1994", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/thsgb-jbd83", "eprint_id": 29428, "eprint_status": "archive", "datestamp": "2023-08-20 04:24:12", "lastmod": "2023-10-24 22:11:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Scherson-T", "name": { "family": "Scherson", "given": "Talma" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Rhombomere rotation reveals that multiple mechanisms contribute to the segmental pattern of hindbrain neural crest migration", "ispublished": "pub", "full_text_status": "public", "keywords": "rhombomere, neural crest, cell migration", "note": "\u00a9 1994 The Company of Biologists Limited.\n\nAccepted 25 March 1994. \n\nWe thank Dr Scott Fraser and Mary Dickinson for invaluable comments on the manuscript and Helene Lesterlin, Simone Lutolf, Roham Zamanian and Parisa Zarbafian for excellent technical assistance with photography, cryosectioning and some embryonic microsurgeries. We also thank Dr Mark Selleck for assistance with some of the computer images. Figures 7B,C were made using an Edge microscope (courtesy of Linda Wegner and Gary Greenberg of Edge Scientific Instrument Corp.) This work was supported by USPHS HD-25138 and a Muscular Dystrophy Grant to M. B.-F.\n\nPublished - SECdev94.pdf
", "abstract": "Hindbrain neural crest cells adjacent to rhombomeres 2 (r2), r4 and r6 migrate in a segmental pattern, toward the first, second and third branchial arches, respectively. Although all rhombomeres generate neural crest cells, those arising from r3 and r5 deviate rostrally and caudally (J. Sechrist, G. Serbedzija, T. Scherson, S. Fraser and M. Bronner-Fraser (1993) Development 118, 691\u2013703). We have altered the rostrocaudal positions of the cranial neural tube, adjacent ectoderm/mesoderm or presumptive otic vesicle to examine tissue influences on this segmental migratory pattern. After neural tube rotation, labeled neural crest cells follow pathways generally appropriate for their new position after grafting. For example, when r3 and r4 were transposed, labeled r3 cells migrated laterally to the second branchial arch whereas labeled r4 cells primarily deviated caudally toward the second arch, with some cells moving rostrally toward the first. In contrast to r4 neural crest cells, transposed r3 cells leave the neural tube surface in a polarized manner, near the r3/4 border. Surprisingly, some labeled neural crest cells moved directionally toward small ectopic otic vesicles that often formed in the ectoderm adjacent to grafted r4. Similarly, they moved toward grafted or displaced otic vesicles. In contrast, surgical manipulation of the mesoderm adjacent to r3 and r4 had no apparent effects. Our results offer evidence that neural crest cells migrate directionally toward the otic vesicle, either by selective attraction or pathway-derived cues.", "date": "1994-07", "date_type": "published", "publication": "Development", "volume": "120", "number": "7", "publisher": "Company of Biologists", "pagerange": "1777-1790", "id_number": "CaltechAUTHORS:20120223-085026338", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120223-085026338", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "Muscular Dystrophy Association" } ] }, "primary_object": { "basename": "SECdev94.pdf", "url": "https://authors.library.caltech.edu/records/thsgb-jbd83/files/SECdev94.pdf" }, "resource_type": "article", "pub_year": "1994", "author_list": "Sechrist, John; Scherson, Talma; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nysfe-wfy54", "eprint_id": 65289, "eprint_status": "archive", "datestamp": "2023-08-20 04:08:11", "lastmod": "2023-10-18 14:29:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Deutzmann-Rainer", "name": { "family": "Deutzmann", "given": "Rainer" } }, { "id": "Perris-Roberto", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural Crest Cell Interactions with Laminin: Structural Requirements and Localization of the Binding Site for \u03b11\u03b21 Integrin", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1994 Academic Press, Inc. \n\nAccepted December 17, 1993. \n\nWe thank Drs. Mats Paulson and Auder Lindblom for generously providing laminin fragments and antisera. We also thank Kristin Bruk Artinger and Brad Martinsen for their technical support. This work was supported by USPHS Grant HD-15527 to M.B.-F.", "abstract": "We have identified the sites of neural crest cell interaction with laminin in vitro by examining their ability to attach to and migrate on proteolytic fragments of the molecule and the ability of fragment-specific antibodies to inhibit these interactions. The binding site on laminin was localized to the E8 domain on the long arm of laminin, as well as the T8\u2032 fragment within this domain, but not the E1\u2032, E3, or E4 fragments. Only subfragments containing the carboxy-terminal rod-like portion of the A chain plus the corresponding B1 and B2 chains retained the attachment-promoting activity of the parent E8 fragment. In addition, interactions required maintenance of the triple-stranded and \u03b1-helical coiled-coil structure of this domain. Reduction and alkylation of laminin and the E8 and T8 fragments significantly reduced neural crest cell attachment and migration. An antiserum against chick \u03b11 integrin reduced migration and adhesion of neural crest cells on an intact laminin-nidogen complex, the E8 fragment, and all its active subfragments. Furthermore, we observed that neural crest cells modified laminin substrata prepared in the absence of divalent cations. Early stable attachment to these substrata was mediated by an integrin other than \u03b11, whereas later attachment and migration were mediated by \u03b11 integrins. Our results suggest that neural crest cells selectively bind to the B1-A-B2 mid-portion (T8\u2032) of the E8 domain of laminin, requiring structural integrity of this region and that they modify laminin substrata as a result of prolonged cell-matrix interactions.", "date": "1994-04", "date_type": "published", "publication": "Developmental Biology", "volume": "162", "number": "2", "publisher": "Elsevier", "pagerange": "451-464", "id_number": "CaltechAUTHORS:20160311-071133334", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160311-071133334", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" } ] }, "doi": "10.1006/dbio.1994.1101", "resource_type": "article", "pub_year": "1994", "author_list": "Lallier, Thomas; Deutzmann, Rainer; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w2jvx-2hp87", "eprint_id": 65223, "eprint_status": "archive", "datestamp": "2023-08-20 03:22:56", "lastmod": "2023-10-18 14:25:55", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Delayed Formation of the Floor Plate after Ablation of the Avian Notochord", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Cell Press. \n\nReceived 17 May 1993, Revised 20 October 1993. \n\nWe would like to thank Drs. S. Fraser, M. Selleck, T. Scherson, J. Sechrist, and the reviewers for helpful comments on the manuscript. We are indebted to Dr. M. Selleck for his skillful technical assistance with the cover artwork. We are grateful to Drs. C. Stern and A. Lumsden for showing us the notochord ablation technique. We thank Dr. M. Tessier-Lavigne and S. Colamarino for help with the collagen gel embedding procedure and Dr. S. Chang for DM-2 antibodies. SC-1, FP-1, and Not-1 antibodies were obtained from Developmental Studies Hybridoma Bank maintained by Johns Hopkins University and the University of Iowa under contract number NO1-HD-2-3144 from the NICHD. This work was supported by USPHS HD-25138. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked \"advertisement\" in accordance with 18 USC Section 1734 solely to indicate this fact.", "abstract": "We have examined the long-term effects of notochord ablation at chick stages 9\u201310 on formation of the floor plate and motor neurons. Although missing or reduced 2 days postablation, the floor plate and motor neurons were morphologically normal by 4 postoperative days. When isolated whole or ventral, but not lateral, neural plate fragments from stage 9 embryos were cultured for 4 days in collagen gels, floor plate and neural markers were observed. Our results suggest that floor plate and motor neurons can form in a delayed fashion in vivo after notochord ablation and in vitro from isolated neural plates. This suggests that either there is an early induction of floor plate by the chordamesoderm of Hensen's node, or only limited interactions between the neural plate and notochord immediately after neurulation are required for floor plate determination.", "date": "1993-12", "date_type": "published", "publication": "Neuron", "volume": "11", "number": "6", "publisher": "Elsevier", "pagerange": "1147-1161", "id_number": "CaltechAUTHORS:20160309-072134200", "issn": "0896-6273", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160309-072134200", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "National Institute of Child Health and Human Development (NICHD)", "grant_number": "NO1-HD-2-3144" }, { "agency": "NIH", "grant_number": "HD-25138" } ] }, "doi": "10.1016/0896-6273(93)90227-I", "resource_type": "article", "pub_year": "1993", "author_list": "Artinger, Kristin B. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0tk2p-zc764", "eprint_id": 29547, "eprint_status": "archive", "datestamp": "2023-08-20 03:20:34", "lastmod": "2023-10-24 22:17:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-R", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Syfrig-J", "name": { "family": "Syfrig", "given": "Josef" } }, { "id": "Paulsson-M", "name": { "family": "Paulsson", "given": "Mats" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular mechanisms of neural crest cell attachment and migration on types I and IV collagen", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest cells, migration, collagen, adhesion", "note": "\u00a9 1993 The Company of Biologists Limited.\n\nReceived 23 July 1993 - Accepted 14 September 1993. \n\nWe are grateful to Drs Klaus K\u00fchn, J\u00f6rgen Wieslander, Helene\nSage, Charles Little, Saryu Dixit and John Harding for providing\nvarious purified intact and fragmented collagens, and anti-collagen\nantibodies. Tammy Dillon, Susan Tran and Kristie Bruk-Artinger are thanked for their invaluable technical assistance. The\nwork was supported from grants from NIH (to M.B.-F.) and from\nthe Swiss National Science Foundation (to M.P.).\n\nPublished - PERdev93.pdf
", "abstract": "We have examined the mechanisms involved in the interaction of avian neural crest cells with collagen types I and IV (Col I and IV) during their adhesion and migration in vitro. For this purpose native Col IV was purified from chicken tissues, characterized biochemically and ultrastructurally. Purified chicken Col I and Col IV, and various proteolytic fragments of the collagens, were used in quantitative cell attachment and migration assays in conjunction with domain-specific collagen antibodies and antibodies to avian integrin subunits. Neural crest cells do not distinguish between different macromolecular arrangements of Col I during their initial attachment, but do so during their migration, showing a clear preference for polymeric Col I. Interaction with Col I is mediated by the \u03b11\u03b21 integrin, through binding to a segment of the \u03b11(I) chain composed of fragment CNBr3. Neural crest cell attachment and migration on Col IV involves recognition of conformation-dependent sites within the triple-helical region and the noncollagenous, carboxyl-terminal NC1 domain. This recognition requires integrity of inter- and intrachain disulfide linkages and correct folding of the molecule. Moreover, there also is evidence that interaction sites within the NC1 domain may be cryptic, being exposed during migration of the cells in the intact collagen as a result of the prolonged cell-substratum contact. In contrast to Col I, neural crest cell interaction with Col IV is mediated by \u03b21-class integrins other than \u03b11\u03b21.", "date": "1993-12", "date_type": "published", "publication": "Journal of Cell Science", "volume": "106", "number": "4", "publisher": "Company of Biologists", "pagerange": "1357-1368", "id_number": "CaltechAUTHORS:20120301-105050290", "issn": "0021-9533", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120301-105050290", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "Swiss National Science Foundation (SNSF)" } ] }, "primary_object": { "basename": "PERdev93.pdf", "url": "https://authors.library.caltech.edu/records/0tk2p-zc764/files/PERdev93.pdf" }, "resource_type": "article", "pub_year": "1993", "author_list": "Perris, Roberto; Syfrig, Josef; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0hjst-gxv34", "eprint_id": 65597, "eprint_status": "archive", "datestamp": "2023-08-20 03:17:58", "lastmod": "2023-10-18 16:10:05", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-Roberto", "name": { "family": "Perris", "given": "Roberto" }, "orcid": "0000-0001-5626-5233" }, { "id": "Kuo-Heuy-Ju", "name": { "family": "Kuo", "given": "Heuy-Ju" } }, { "id": "Glanville-Robert-W", "name": { "family": "Glanville", "given": "Robert W." } }, { "id": "Leibold-Scott", "name": { "family": "Leibold", "given": "Scott" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural Crest Cell Interaction with Type VI Collagen Is Mediated by Multiple Cooperative Binding Sites within Triple-Helix and Globular Domains", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Academic Press. \n\nWe are indebted to Douglas R. Keene for the transmission electron microscopy, Dr. Rupert Timpl for providing the recombinant N2-N9 fragment, Dr. Eva Engvall for providing mAbs 2C6 and 5C6, and Dr. Alfonso Colombatti for comments on the manuscript. We like to thank Kristin Artinger, Cynthia A. Bohan, Susan DeMaggio, Bruce Donaldson, Brad Martinsen, and Erik L. Snapp for their excellent technical assistance. The electron microscope facility at The Shriners\nHospital was supported in part by grants from the Fred Mayer Charitable Trust and R. Blaire Bramble Medical Research Foundation. The work was supported in part by grants from USPHS, Grant HD-15527 to M.B.F., and a grant from the Shriners Hospital for Crippled Children to R.W.G.", "abstract": "Collagen type VI (Col VI) is a primary constituent of the extracellular matrix encountered by migrating avian neural crest cells in situ and is effective in promoting attachment and motility of these cells in vitro. In this study, we have explored the molecular mechanisms of neural crest-Col VI interaction by using quantitative assays for cell attachment and migration in vitro, proteolytic fragments of the collagen, and a panel of domain-specific monoclonal antibodies. Removal of the predominant portion of the amino-terminal globular domains of Col VI tetramers by pepsin digestion (P6 fragment) resulted in a > fivefold decrease in their cell adhesion and motility-promoting activity. Further digestion of P6 with bacterial collagenase, which causes a complete loss of the amino-terminal domains plus an adjacent triple-helical segment, did not affect adhesion but reduced migration down to 40% of that seen on undigested P6. Untreated and pepsin-digested Col VI monomers were significantly less effective than their tetrameric counterparts and a M_r 200,000 fragment, generated from pepsin-digested monomers by a second pepsin treatment, only retained 40% of the motilitypromoting activity while preserving the adhesive capacity. A mixture of amino- and carboxyl-terminal globular domains supported both cell attachment and migration. While neural crest cells adhered equally well to the individual intact \u03b11(VI)/\u03b12(VI) and \u03b13(VI) chains, they migrated most extensively on the \u03b13(VI) chain. Conversely, pepsin-digested individual a chains were significantly less effective in promoting cell adhesion and locomotion. Selective preincubation of Col VI microfilaments and isolated tetramers with a panel of monoclonal antibodies against triple helix, carboxylterminal, and amino-terminal epitopes of the different constituent chains differentially perturbed neural crest cell attachment and migration. Sites differentially involved in neural crest cell attachment and migration seemed to be present at the carboxyl termini of the \u03b11(VI) and \u03b12(VI) chains and at the amino-terminus of the \u03b13(VI) chain. The results suggest that neural crest cells interact with Col VI through multiple and cooperative binding sites present within its triple-helical and globular domains. The differential involvement and efficiency of these sites in stimulating neural crest cell adhesion and migration is strongly determined by the supramolecular organization of the collagen and requires inter- and intramolecular structural integrity. Since neural crest cell attachment and migration on Col VI was completely inhibited by anti-\u03b2_1 integrin antibodies, there is evidence that this class of integrins is essential for the neural crest cell-Col VI interaction.", "date": "1993-11-01", "date_type": "published", "publication": "Experimental Cell Research", "volume": "209", "number": "1", "publisher": "Elsevier", "pagerange": "103-117", "id_number": "CaltechAUTHORS:20160322-130128162", "issn": "0014-4827", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160322-130128162", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Fred Mayer Charitable Trust" }, { "agency": "R. Blaire Bramble Medical Research Foundation" }, { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "Shriners Hospital for Crippled Children" } ] }, "doi": "10.1006/excr.1993.1290", "resource_type": "article", "pub_year": "1993", "author_list": "Perris, Roberto; Kuo, Heuy-Ju; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/f6zzq-2wd49", "eprint_id": 65259, "eprint_status": "archive", "datestamp": "2023-08-20 03:16:26", "lastmod": "2023-10-18 14:27:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural crest cell migration in the developing embryo", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Elsevier Science Publishers Ltd (UK). \n\nOur work is supported by USPHS HD-15527, DE10G66 and a grant from the Muscular Dystrophy Foundation.", "abstract": "In vertebrate embryos, neural crest cells migrate extensively to defined sites where they differentiate into a complex array of derivatives, ranging from neurons to pigment cells. Neural crest cells emerge uniformly from the neural tube but their subsequent migratory pattern is segmented along much of the body axis. What factors control this segmental migration? At trunk levels, it is imposed by the intrinsic segmentation of the neighbouring somitic mesoderm, while in the head, intrinsic information within the neural tube as well as extrinsic influences from the ectoderm are involved. A variety of cell-cell and cell-extracellular matrix interactions are thought to influence initiation and movement of neural crest cells. This review summarizes recent progress from both experimental embryology and cell biology approaches in uncovering the mechanisms underlying neural crest cell migration.", "date": "1993-11", "date_type": "published", "publication": "Trends in Cell Biology", "volume": "3", "number": "11", "publisher": "Elsevier", "pagerange": "392-397", "id_number": "CaltechAUTHORS:20160310-120052308", "issn": "0962-8924", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160310-120052308", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "DE10G66" }, { "agency": "Muscular Dystrophy Foundation" } ] }, "doi": "10.1016/0962-8924(93)90089-J", "resource_type": "article", "pub_year": "1993", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fbqzb-nda42", "eprint_id": 65931, "eprint_status": "archive", "datestamp": "2023-08-22 09:34:48", "lastmod": "2023-10-18 16:56:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-R", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Kuo-Heuy-Ju", "name": { "family": "Kuo", "given": "Heuy-Ju" } }, { "id": "Glanville-R-W", "name": { "family": "Glanville", "given": "Robert W." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Collagen Type VI in Neural Crest Development: Distribution In Situ and Interaction With Cells In Vitro", "ispublished": "pub", "full_text_status": "restricted", "keywords": "Neural crest; Collagen VI; Migration; Adhesion; Distribution", "note": "\u00a9 1993 Wiley-Liss, Inc. \n\nReceived June 28, 1993; accepted August 26, 1993. Article first published online: 3 Feb 2005. \n\nThe authors wish to thank Dr. Alfonso Colombatti for providing purified chicken Col VI, antibodies to chicken Col VI and for critical reading of the manuscript. We are indebted to Douglas Keene for performing the electron microscopy at The Shriners Hospital's Facilities which are supported in part by grants from the R. Blaine Bramble Medical Research Foundation and the Fred Mayer Charitable Trust. Scott Leibold, Tammy Dillon and Susan De Maggio are thanked for their assistance with cell adhesion assays and video time-lapse microscopy. Kristin Artinger, Bruce Donaldson, Laura Magris, and Paolo Pontonutti are gratefully acknowledged for their excellent technical assistance. \n\nThe work was supported in part by grants USPHS HD-15527 to M.B.-F. and from the Shriners Hospital for Crippled Children to R.W.G.", "abstract": "We have examined the spatiotemporal distribution of collagen type VI (Col VI) during neural crest development in vivo and its ability to promote neural crest cell attachment and migration in vitro. An affinity purified antiserum and chain-specific monoclonal antibodies against chicken Col VI were employed to immunolocalize the collagen in tissue sections and by immunoblotting. At stages of initial neural crest cell migration, the \u03b11(VI) and \u03b12(VI) chains were immunolocalized in apposition with basement membrances of the neural tube, somites, notochord and ectoderm, whereas no immunoreactivity was seen for the \u03b13(VI) chain. Immunoblotting analysis confirmed the expression of \u03b11(VI) and \u03b12(VI) chains and the lack of detectable immunoreactivity for the \u03b13(VI) chain at these early phases of neural crest development. Conversely, at advanced phases of migration and following gangliogenesis, expression of \u03b13(VI) chain coincided with that of \u03b11(VI) and \u03b12(VI) chains in apposition with basement membrances, around the dorsal root ganglia, and in fibrillar arrangements within the developing dermis and ventral sclerotome. The ability of Col VI to promote neural crest cell attachment and migration was tested in vitro using quantitative assays for these processes. Both native microfilaments and isolated tetramers of Col VI strongly promoted neural crest cell attachment and migration. Optimal stimulation of neural crest cell adhesion and migration was dependent upon structural integrity of Col VI since unfolded and disassembled \u03b1 chains only weakly promoted cell attachment and were virtually inactive in supporting cell movement. The importance of a native macromolecular organization of Col VI further was analyzed in experiments in which dissociated tetramers were reassociated by Ca^(2+)- and temperature-dependent self-aggregation. In contrast to native microfilaments, these oligomeric complexes were less effective in promoting neural crest cell movement, but still retained the ability to stimulate maximal cell attachment. The results indicate that Col VI is a primary component of the extracellular matrix deposited along neural crest migratory pathways, where it may participate in the regulation of cell movement by functioning as a migratory substrate. The ability of Col VI to promote neural crest cell adhesion and motility is highly dependent upon maintainance of a native macromolecular arrangement.", "date": "1993-10", "date_type": "published", "publication": "Developmental Dynamics", "volume": "198", "number": "2", "publisher": "Wiley-Liss, Inc.", "pagerange": "135-149", "id_number": "CaltechAUTHORS:20160405-113538594", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160405-113538594", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "R. Blaine Bramble Medical Research Foundation" }, { "agency": "Fred Mayer Charitable Trust" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Shriners Hospital for Crippled Children" } ] }, "doi": "10.1002/aja.1001980207", "resource_type": "article", "pub_year": "1993", "author_list": "Perris, Roberto; Kuo, Heuy-Ju; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cv7r3-vfc85", "eprint_id": 65292, "eprint_status": "archive", "datestamp": "2023-08-20 03:05:57", "lastmod": "2023-10-18 14:30:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Selleck-M-A-J", "name": { "family": "Selleck", "given": "Mark A. J." } }, { "id": "Scherson-T-Y", "name": { "family": "Scherson", "given": "Talma Y." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Origins of Neural Crest Cell Diversity", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Academic Press, Inc. \n\nAccepted May 28, 1993. \n\nWe are indebted to Drs. Scott Fraser, Zoe Pettway, John Sechrist, and Claudio Stern, and Kristin Artinger for stimulating discussion and helpful comments on the manuscript. M.A.J.S. is supported by a Muscular Dystrophy Association research fellowship and T.Y.S. is supported by NIH Grant HD-25138. Some of the experiments in this review were funded by HD-25138 and a grant from the Muscular Dystrophy Association to M.B.-F.", "abstract": "The neural crest is a population of migratory cells, arising from the ectoderm, that invades many sites within the embryo and differentiate into a variety of diverse cell types. Pigment cells, most cells of the peripheral nervous system, adrenal medullary cells, and some cranial cartilage are derived from the neural crest. Despite a wealth of knowledge concerning their pathways of migration and vast array of derivatives, little is known about the formation of neural crest cells or their acquisition of positional identity. This review focuses on the origin of neural crest cells from the ectoderm and the generation of differences in neural crest cell fates along the rostrocaudal axis. In addition, we consider the role of temporal restriction in the developmental potential of premigratory neural crest cells. While evidence for the existence of multipotent stem cells is strong, some experiments also suggest that there may be heterogeneity among neural crest cell precursors, perhaps due to differences in origin, that might explain commitment events occurring early in neural crest development.", "date": "1993-09", "date_type": "published", "publication": "Developmental Biology", "volume": "159", "number": "1", "publisher": "Elsevier", "pagerange": "1-11", "id_number": "CaltechAUTHORS:20160311-075507536", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160311-075507536", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Muscular Dystrophy Association" }, { "agency": "NIH", "grant_number": "HD-25138" } ] }, "doi": "10.1006/dbio.1993.1217", "resource_type": "article", "pub_year": "1993", "author_list": "Selleck, Mark A. J.; Scherson, Talma Y.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/j0vye-xez76", "eprint_id": 29533, "eprint_status": "archive", "datestamp": "2023-08-20 02:58:41", "lastmod": "2023-10-24 22:17:06", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Scherson-T", "name": { "family": "Scherson", "given": "Talma" } }, { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott" }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Regulative capacity of the cranial neural tube to form neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "cell lineage, ablation, regeneration, respecification, cell marking techniques, regulation, chick", "note": "\u00a9 1993 The Company of Biologists Limited.\n\nAccepted 30 April 1993. \n\nWe thank Dr John Sechrist helpful comments, suggestions and\ntechnical input, Dr Mark Selleck for helpful comments on the manuscript\nand Simone Lutolf for assistance with cryosectioning. This\nwork was supported by USPHS grant HD-25138 to M. B.-F. and\nHD-29304 to S. E. F.\n\nPublished - SCHEdev93.pdf
", "abstract": "In avian embryos, cranial neural crest cells emigrate from the dorsal midline of the neural tube shortly after neural tube closure. Previous lineage analyses suggest that the neural crest is not a pre-segregated population of cells within the neural tube; instead, a single progenitor in the dorsal neural tube can contribute to neurons in both the central and the peripheral nervous systems (Bronner-Fraser and Fraser, 1989 Neuron 3, 755\u2013766). To explore the relationship between the 'premigratory' neural crest cells and the balance of the cells in the neural tube in the midbrain and hindbrain region, we have challenged the fate of these populations by ablating the neural crest either alone or in combination with the adjoining ventral portions of the neural tube. Focal injections of the vital dye, DiI, into the neural tissue bordering the ablated region demonstrate that cells at the same axial level, in the lateral and ventral neural tube, regulate to reconstitute a population of neural crest cells. These cells emigrate from the neural tube, migrate along normal pathways according to their axial level of origin and appear to give rise to a normal range of derivatives. This regulation following ablation suggests that neural tube cells normally destined to form CNS derivatives can adjust their prospective fates to form PNS and other neural crest derivatives until 4.5-6 hours after the time of normal onset of emigration from the neural tube.", "date": "1993-08", "date_type": "published", "publication": "Development", "volume": "118", "number": "4", "publisher": "Company of Biologists", "pagerange": "1049-1061", "id_number": "CaltechAUTHORS:20120229-153248605", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120229-153248605", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "HD-29304" } ] }, "primary_object": { "basename": "SCHEdev93.pdf", "url": "https://authors.library.caltech.edu/records/j0vye-xez76/files/SCHEdev93.pdf" }, "resource_type": "article", "pub_year": "1993", "author_list": "Scherson, Talma; Serbedzija, George; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/26h8y-p2496", "eprint_id": 29543, "eprint_status": "archive", "datestamp": "2023-08-20 02:55:35", "lastmod": "2023-10-24 22:17:25", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "J." } }, { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "G. N." } }, { "id": "Scherson-T", "name": { "family": "Scherson", "given": "T." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "S. E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Segmental migration of the hindbrain neural crest does not arise from its segmental generation", "ispublished": "pub", "full_text_status": "public", "keywords": "neurofilament proteins, DiI labelling, rhombomeres, segmentation, hindbrain", "note": "\u00a9 1993 The Company of Biologists Limited.\n\nAccepted 15 April 1993. \n\nWe thank Mary Flowers for excellent technical assistance and\nDr Mark Selleck and John Wolf for help with some of the DiI injections.\nWe greatly appreciate the critical comments of Drs Andrew\nLumsden, Mark Selleck and Claudio Stern on the manuscript. This\nstudy was supported by USPHS HD-25138 to M. B.-F. and HD-29304 to S. E. F.\n\nPublished - SECdev93.pdf
", "abstract": "The proposed pathways of chick cranial neural crest migration and their relationship to the rhombomeres of the hindbrain have been somewhat controversial, with differing results emerging from grafting and DiI-labelling analyses. To resolve this discrepancy, we have examined cranial neural crest migratory pathways using the combination of neurofilament immunocytochemistry, which recognizes early hindbrain neural crest cells, and labelling with the vital dye, DiI. Neurofilament-positive cells with the appearance of premigratory and early-migrating neural crest cells were noted at all axial levels of the hindbrain. At slightly later stages, neural crest cell migration in this region appeared segmented, with no neural crest cells obvious in the mesenchyme lateral to rhombomere 3 (r3) and between the neural tube and the otic vesicle lateral to r5. Focal injections of DiI at the levels of r3 and r5 demonstrated that both of these rhombomeres generated neural crest cells. The segmental distribution of neural crest cells resulted from the DiI-labelled cells that originated in r3 and r5 deviating rostrally or caudally and failing to enter the adjacent preotic mesoderm or otic vesicle region. The observation that neural crest cells originating from r3 and r5 avoided specific neighboring domains raises the intriguing possibility that, as in the trunk, extrinsic factors play a major role in the axial patterning of the cranial neural crest and the neural crest-derived peripheral nervous system.", "date": "1993-07-01", "date_type": "published", "publication": "Development", "volume": "118", "number": "3", "publisher": "Company of Biologists", "pagerange": "691-703", "id_number": "CaltechAUTHORS:20120301-092619387", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120301-092619387", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "NIH", "grant_number": "HD-29304" } ] }, "primary_object": { "basename": "SECdev93.pdf", "url": "https://authors.library.caltech.edu/records/26h8y-p2496/files/SECdev93.pdf" }, "resource_type": "article", "pub_year": "1993", "author_list": "Sechrist, J.; Serbedzija, G. N.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/63akg-mag19", "eprint_id": 29536, "eprint_status": "archive", "datestamp": "2023-08-20 02:44:56", "lastmod": "2023-10-24 22:17:11", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Collazo-A", "name": { "family": "Collazo", "given": "Andres" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Vital dye labelling of Xenopus laevis trunk neural crest reveals multipotency and novel pathways of migration", "ispublished": "pub", "full_text_status": "public", "keywords": "DiI, developmental potential, clonal analysis, LRD, Xenopus neural crest, vital dye labelling, neural crest", "note": "\u00a9 1993 The Company of Biologists Limited.\n\nAccepted 9 March 1993. \n\nWe thank Tina Joe, Gary Belford, Mary Flowers and Forrest\nVickery for technical assistance and Susana Cohen-Cory, Jack\nSechrist, John Shih and Claudio Stern for critical reading of the\nmanuscript. This work was supported by USPHS grants (HD25138\nto M. B.-F.; HD26864 to S. E. F.) and fellowship support from\nthe NIH (1F32NS09140-01) and the Muscular Dystrophy Association\nto A. C.\n\nPublished - COLdev93.pdf
", "abstract": "Although the Xenopus embryo has served as an important model system for both molecular and cellular studies of vertebrate development, comparatively little is known about its neural crest. Here, we take advantage of the ease of manipulation and relative transparency of Xenopus laevis embryos to follow neural crest cell migration and differentiation in living embryos. We use two techniques to study the lineage and migratory patterns of frog neural crest cells: (1) injections of DiI or lysinated rhodamine dextran (LRD) into small populations of neural crest cells to follow movement and (2) injections of LRD into single cells to follow cell lineage. By using non-invasive approaches that allow observations in living embryos and control of the time and position of labelling, we have been able to expand upon the results of previous grafting experiments. Migration and differentiation of the labelled cells were observed over time in individual living embryos, and later in sections to determine precise position and morphology. Derivatives populated by the neural crest are the fins, pigment stripes, spinal ganglia, adrenal medulla, pronephric duct, enteric nuclei and the posterior portion of the dorsal aorta. In the rostral to mid-trunk levels, most neural crest cells migrate along two paths: a dorsal pathway into the fin, followed by presumptive fin cells, and a ventral pathway along the neural tube and notochord, followed by presumptive pigment, sensory ganglion, sympathetic ganglion and adrenal medullary cells. In the caudal trunk, two additional paths were noted. One group of cells moves circumferentially within the fin, in an arc from dorsal to ventral; another progresses ventrally to the anus and subsequently populates the ventral fin. By labelling individual precursor cells, we find that neural tube and neural crest cells often share a common precursor. The majority of clones contain labelled progeny cells in the dorsal fin. The remainder have progeny in multiple derivatives including spinal ganglion cells, pigment cells, enteric cells, fin cells and/or neural tube cells in all combinations, suggesting that many premigratory Xenopus neural crest precursors are multipotent.", "date": "1993-06", "date_type": "published", "publication": "Development", "volume": "118", "number": "2", "publisher": "Company of Biologists", "pagerange": "363-376", "id_number": "CaltechAUTHORS:20120301-073849425", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120301-073849425", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD25138" }, { "agency": "NIH", "grant_number": "HD26864" }, { "agency": "NIH", "grant_number": "1F32NS09140-01" }, { "agency": "Muscular Dystrophy Association" } ] }, "primary_object": { "basename": "COLdev93.pdf", "url": "https://authors.library.caltech.edu/records/63akg-mag19/files/COLdev93.pdf" }, "resource_type": "article", "pub_year": "1993", "author_list": "Collazo, Andres; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ab8zm-p1k72", "eprint_id": 65226, "eprint_status": "archive", "datestamp": "2023-08-20 02:33:15", "lastmod": "2023-10-18 14:26:03", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Crest destiny", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Current Biology. \n\nI thank Dr. Mark Selleck for his critical comments on the manuscript, and the National Institutes of Health and Muscular Dystrophy Association for grant support.", "abstract": "Stem cells with a limited capacity for renewal have been isolated from mammalian neural crest. Manipulation of these cells may clarify the relationship between neuronal and glial cell fates.", "date": "1993-04", "date_type": "published", "publication": "Current Biology", "volume": "3", "number": "4", "publisher": "Cell Press", "pagerange": "201-203", "id_number": "CaltechAUTHORS:20160309-075030052", "issn": "0960-9822", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160309-075030052", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "Muscular Dystrophy Association" } ] }, "doi": "10.1016/0960-9822(93)90332-I", "resource_type": "article", "pub_year": "1993", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qv0fa-d1x45", "eprint_id": 63294, "eprint_status": "archive", "datestamp": "2023-08-20 02:32:53", "lastmod": "2023-10-25 23:42:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Mechanisms of neural crest cell migration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1993 Cambridge University Press.", "abstract": "Neural crest cells are remarkable in their extensive and stereotypic patterns of migration. The pathways of neural crest migration have been documented by cell marking techniques, including interspecific neural tube grafts, immunocytochemistry and Dil-labelling. In the trunk, neural crest cells migrate dorsally under the skin or ventrally through the somites, where they move in a segmental fashion through the rostral half of each sclerotome. The segmental migration of neural crest cells appears to be prescribed by the somites, perhaps by an inhibitory cue from the caudal half. Within the rostral sclerotome, neural crest cells fill the available space except for a region around the notochord, suggesting the notochord may inhibit neural crest cells in its vicinity. In the cranial region, antibody perturbation experiments suggest that multiple cell-matrix interactions are required for proper in vivo migration of neural crest cells. Neural crest cells utilize integrin receptors to bind to a number of extracellular matrix molecules. Substrate selective inhibition of neural crest cell attachment in vitro by integrin antibodies and antisense oligonucleotides has demonstrated that they possess at least three integrins, one being an \u03b1_1\u03b2_1 integrin which functions in the absence of divalent cations. Thus, neural crest cells utilize complex sets of interactions which may differ at different axial levels.", "date": "1993-04", "date_type": "published", "publication": "Bioessays", "volume": "15", "number": "4", "publisher": "Cambridge University Press", "pagerange": "221-230", "id_number": "CaltechAUTHORS:20160101-182353816", "issn": "0265-9247", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-182353816", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/bies.950150402", "resource_type": "article", "pub_year": "1993", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/ggeza-3yk50", "eprint_id": 63293, "eprint_status": "archive", "datestamp": "2023-08-22 09:14:15", "lastmod": "2023-10-25 23:42:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Environmental influences on neural crest cell migration", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest cell migration; extracellular matrix molecules; cell marking techniques; notochord inhibitory effects", "note": "\u00a9 1993 John Wiley & Sons. \n\nReceived September 8, 1992; accepted September 8, 1992. \n\nParts of the work described in this review were supported by USPHS grant HD-15527.", "abstract": "Neural crest cells migrate extensively and interact with numerous tissues and extracellular matrix components during their movement. Cell marking techniques have shown that neural crest cells in the trunk of the avian embryo migrate through the anterior, but not posterior, half of each sclerotome and avoid the region around the notochord. A possible mechanism to account for this migratory pattern is that neural crest cells may be inhibited from entering the posterior sclerotome and the perinotochordal space. Thus, interactions with other tissue may prescribe the pattern of neural crest cell migration in the trunk. In contrast, interactions between neural crest cells and the extracellular matrix may mediate the primary interactions controlling neural crest cells migration in the head region.", "date": "1993-02", "date_type": "published", "publication": "Journal of Neurobiology", "volume": "24", "number": "2", "publisher": "Wiley", "pagerange": "233-247", "id_number": "CaltechAUTHORS:20160101-182142774", "issn": "0022-3034", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-182142774", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" } ] }, "doi": "10.1002/neu.480240209", "resource_type": "article", "pub_year": "1993", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nsp1t-wr657", "eprint_id": 65970, "eprint_status": "archive", "datestamp": "2023-08-20 02:19:40", "lastmod": "2023-10-18 17:00:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Inhibition of Neural Crest Cell Attachment by Integrin Antisense Oligonucleotides", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1991 American Association for the Advancement of Science. \n\n22 April 1992; accepted 2 December 1992. \n\nWe thank S. Fraser for comments on the manuscript. Supported by USPHS-15527 to MB-F.", "abstract": "Neural crest cell interactions with extracellular matrix molecules were analyzed with the use of antisense oligonucleotides to block synthesis of integrin subunits. When added to the culture medium of quail neural crest cells, selected antisense phosphorothiol oligonucleotides reduced the amounts of cell surface \u0251 or \u03b2 integrin subunits by up to 95 percent and inhibited neural crest cell attachment to laminin or fibronectin substrata. Differential effects on specific alpha integrins were noted after treatment with \u0251-specific oligonucleotides. Cells recovered the ability to bind to substrata 8 to 16 hours after treatment with inhibitory oligonucleotides. The operation of at least three distinct \u0251 integrin subunits is indicated by substratum-selective inhibition of cell attachment.", "date": "1993-01-29", "date_type": "published", "publication": "Science", "volume": "259", "number": "5095", "publisher": "American Association for the Advancement of Science", "pagerange": "692-695", "id_number": "CaltechAUTHORS:20160406-140352440", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160406-140352440", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" } ] }, "doi": "10.1126/science.8430321", "resource_type": "article", "pub_year": "1993", "author_list": "Lallier, Thomas and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/h1005-jxg29", "eprint_id": 65090, "eprint_status": "archive", "datestamp": "2023-08-20 02:09:31", "lastmod": "2023-10-17 23:10:57", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Segregation of cell lineage in the neural crest", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1993 Current Biology Ltd.", "abstract": "Following neurulation, neural crest cells emerge from the neural tube and undergo extensive migrations. At the onset of migration, multipotent stem cells exist within the neural crest population. Eventually, these assume one of a number of possible fates, ranging from neurons and glia of the peripheral nervous system to pigment cells and cells of the facial skeleton. Neural crest cells follow migratory pathways and differentiate into derivatives that often are characteristic of their axial level of origin. Based on their stereotyped patterns of migration, limited intermixing and distinct homeobox-gene codes, some populations of neural crest cells may have a rostrocaudal regional identity imprinted prior to their emigration.", "date": "1993", "date_type": "published", "publication": "Current Opinion in Genetics and Development", "volume": "3", "number": "4", "publisher": "Current Biology Ltd", "pagerange": "641-647", "id_number": "CaltechAUTHORS:20160304-164707764", "issn": "0959-437X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160304-164707764", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1016/0959-437X(93)90101-T", "resource_type": "article", "pub_year": "1993", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8ckhg-9cd10", "eprint_id": 66077, "eprint_status": "archive", "datestamp": "2023-08-20 01:58:17", "lastmod": "2023-10-18 17:07:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "\u0251\u2081\u03b2\u2081 integrin on neural crest cells recognizes some laminin substrata in a Ca\u00b2\u207a-independent manner", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1992 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nReceived for publication 2 March 1992 and in revised form 21 August\n1992. \n\nWe thank Dr. Scott Fraser for helpful comments on the manuscript, Dr. Mats Paulsson for his generous gift of the anti-\u0251_1 integrin antiserum and Dr. Friedrich Bonhoeffer for kindly providing the silicon molds for preparing the lane assays. This work was supported by Public Health Services grant HD15527 to M. Brenner-Fraser.\n\nPublished - J_Cell_Biol-1992-Lallier-1335-45.pdf
", "abstract": "Neural crest cells migrate along pathways containing laminin and other extracellular matrix molecules. In the present study, we functionally and biochemically identify an \u0251\u2081\u03b2\u2081 integrin heterodimer which bears the HNK-1 epitope on neural crest cells. Using a quantitative cell adhesion assay, we find that this heterodimer mediates attachment to laminin substrata prepared in the presence of Ca\u00b2\u207a. Interestingly, neural crest cells bind to laminin-Ca\u00b2\u207a substrata in the presence or absence of divalent cations in the cell attachment medium. In contrast, the attachment of neural crest cells to laminin substrata prepared in the presence of EDTA, heparin, Mg\u00b2\u207a, or Mn\u00b2\u207a requires divalent cations. Interactions with these laminin substrata are mediated by a different integrin heterodimer, since antibodies against \u03b2\u2081 but not \u0251\u2081 integrins inhibit neural crest cell attachment. Thus, the type of laminin substratum appears to dictate the choice of laminin receptor used by neural crest cells. The laminin conformation is determined by the ratio of laminin to Ca\u00b2\u207a, though incorporation of heparin during substratum polymerization alters the conformation even in the presence of Ca\u00b2\u207a. Once polymerized, the substratum appears stable, not being altered by soaking in either EDTA or divalent cations. Our findings demonstrate: (a) that the \u0251\u2081\u03b2\u2081 integrin can bind to some forms of laminin in the absence of soluble divalent cations; (b) that substratum preparation conditions alter the conformation of laminin such that plating laminin in the presence of Ca\u00b2\u207a and/or heparin modulates its configuration; and (c) that neural crest cells utilize different integrins to recognize different laminin conformations.", "date": "1992-12-01", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "119", "number": "5", "publisher": "Rockefeller University Press", "pagerange": "1335-1345", "id_number": "CaltechAUTHORS:20160412-093144468", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160412-093144468", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD15527" } ] }, "doi": "10.1083/jcb.119.5.1335", "pmcid": "PMC2289724", "primary_object": { "basename": "J_Cell_Biol-1992-Lallier-1335-45.pdf", "url": "https://authors.library.caltech.edu/records/8ckhg-9cd10/files/J_Cell_Biol-1992-Lallier-1335-45.pdf" }, "resource_type": "article", "pub_year": "1992", "author_list": "Lallier, Thomas and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/g9yyv-gdm37", "eprint_id": 12382, "eprint_status": "archive", "datestamp": "2023-08-22 09:05:49", "lastmod": "2023-10-17 16:43:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Notochord grafts do not suppress formation of neural crest cells or commissural neurons", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest cells, dorsal root ganglion, commissural neurons, ventral roots", "note": "Copyright \u00a9 1992 by Company of Biologists. \n\n(Accepted 9 September 1992) \n\nWe thank Drs Scott Fraser, Mark Selleck, John Sechrist and Zoe Pettway for helpful comments on the manuscript, Dr John Sechrist for helping with analysis of neurofilament immunoreactivity and Mary Flowers for her technical assistance. We gratefully acknowledge the generous gifts of antibodies from a number of colleagues: Drs H. Tanaka and T. Yamada for the SC1 antibody, Dr Virginia Lee for the neurofilament antibody and Drs Ulf Eriksson and Malcolm Maden for the anti-CRABP antibodies. This work was supported by USPHS HD-25138.\n\nPublished - ARTdev92.pdf
", "abstract": "Grafting experiments previously have established that the notochord affects dorsoventral polarity of the neural tube by inducing the formation of ventral structures such as motor neurons and the floor plate. Here, we examine if the notochord inhibits formation of dorsal structures by grafting a notochord within or adjacent to the dorsal neural tube prior to or shortly after tube closure. In all cases, neural crest cells emigrated from the neural tube adjacent to the ectopic notochord. When analyzed at stages after ganglion formation, the dorsal root ganglia appeared reduced in size and shifted in position in embryos receiving grafts. Another dorsal cell type, commissural neurons, identified by CRABP and neurofilament immunoreactivity, differentiated in the vicinity of the ectopic notochord. Numerous neuronal cell bodies and axonal processes were observed within the induced, but not endogenous, floor plate 1 to 2 days after implantation but appeared to be cleared with time. These results suggest that dorsally implanted notochords cannot prevent the formation of neural crest cells or commissural neurons, but can alter the size and position of neural crest-derived dorsal root ganglia.", "date": "1992-12", "date_type": "published", "publication": "Development", "volume": "116", "number": "4", "publisher": "Company of Biologists", "pagerange": "877-887", "id_number": "CaltechAUTHORS:ARTdev92", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:ARTdev92", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" } ] }, "primary_object": { "basename": "ARTdev92.pdf", "url": "https://authors.library.caltech.edu/records/g9yyv-gdm37/files/ARTdev92.pdf" }, "resource_type": "article", "pub_year": "1992", "author_list": "Artinger, Kristin B. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/8j6mh-1ne58", "eprint_id": 29737, "eprint_status": "archive", "datestamp": "2023-08-20 01:49:53", "lastmod": "2023-10-24 22:24:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Leblanc-Gabrielle", "name": { "family": "Leblanc", "given": "Gabrielle" } }, { "id": "Artinger-Kristin-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices", "ispublished": "pub", "full_text_status": "public", "keywords": "integrins, cell adhesion, laminin, fibronectin, collagen", "note": "\u00a9 1992 The Company of Biologists Limited.\n\nAccepted 19 August 1992. \n\nThis work was supported by USPHS grant HD-15527 to M. B. F. and BNS-8702512 to G. G. L.\n\nPublished - LALdev92.pdf
", "abstract": "We have used a quantitative cell attachment assay to compare the interactions of cranial and trunk neural crest cells with the extracellular matrix (ECM) molecules fibronectin, laminin and collagen types I and IV. Antibodies to the \u03b2\u2081 subunit of integrin inhibited attachment under all conditions tested, suggesting that integrins mediate neural crest cell interactions with these ECM molecules. The HNK-1 antibody against a surface carbohydrate epitope under certain conditions inhibited both cranial and trunk neural crest cell attachment to laminin, but not to fibronectin. An antiserum to \u03b1\u2081 intergrin inhibited attachment of trunk, but not cranial, neural crest cells to laminin and collagen type I, though interactions with fibronectin or collagen type IV were unaffected. The surface properties of trunk and cranial neural crest cells differed in several ways. First, trunk neural crest cells attached to collagen types I and IV, but cranial neural crest cells did not. Second, their divalent cation requirements for attachment to ECM molecules differed. For fibronectin substrata, trunk neural crest cells required divalent cations for attachment, whereas cranial neural crest cells bound in the absence of divalent cations. However, cranial neural crest cells lost this cation-independent attachment after a few days of culture. For laminin substrata, trunk cells used two integrins, one divalent cation-dependent and the other divalent cation-independent (Lallier, T. E. and Bronner-Fraser, M. (1991) Development 113, 1069\u20131081). In contrast, cranial neural crest cells attached to laminin using a single, divalent cation-dependent receptor system. Immunoprecipitations and immunoblots of surface labelled neural crest cells with HNK-1, \u03b1\u2081 integrin and \u03b2\u2081 integrin antibodies suggest that cranial and trunk neural crest cells possess biochemically distinct integrins. Our results demonstrate that cranial and trunk cells differ in their mechanisms of adhesion to selected ECM components, suggesting that they are non-overlapping populations of cells with regard to their adhesive properties.", "date": "1992-11", "date_type": "published", "publication": "Development", "volume": "116", "number": "3", "publisher": "Company of Biologists", "pagerange": "531-541", "id_number": "CaltechAUTHORS:20120315-154915681", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120315-154915681", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "NSF", "grant_number": "BNS-8702512" } ] }, "doi": "10.1242/dev.116.3.531", "primary_object": { "basename": "LALdev92.pdf", "url": "https://authors.library.caltech.edu/records/8j6mh-1ne58/files/LALdev92.pdf" }, "resource_type": "article", "pub_year": "1992", "author_list": "Lallier, Thomas; Leblanc, Gabrielle; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/n5g1g-8tt84", "eprint_id": 65168, "eprint_status": "archive", "datestamp": "2023-08-20 01:45:55", "lastmod": "2023-10-17 23:14:48", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Wolf-J-J", "name": { "family": "Wolf", "given": "John J." } }, { "id": "Murray-B-A", "name": { "family": "Murray", "given": "Ben A." } } ] }, "title": "Effects of Antibodies against N-Cadherin and N-CAM\n on the Cranial Neural Crest and Neural Tube", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1992 Academic Press, Inc. \n\nAccepted 8 May 1992. \n\nWe thank Drs. Masatoshi Takeichi, Jack Lillien, and David Gottlieb for kindly providing antibodies used in this study and Brad Martinsen for excellent technical assistance. This work was supported by USPHS Grant DE10066 to M.B.-F. and by American Cancer Society Grant CD-416 to B.A.M.", "abstract": "We have examined the distribution and function of the defined cell adhesion molecules, N-cadherin and N-CAM, in the emigration of cranial neural crest cells from the neural tube in vivo. By immunocytochemical analysis, both N-cadherin and N-CAM were detected on the cranial neural folds prior to neural tube closure. After closure of the neural tube, presumptive cranial neural crest cells within the dorsal aspect of the neural tube had bright N-CAM and weak N-cadherin immunoreactivity. By the 10- to 11-somite stage, N-cadherin was prominent on all neural tube cells with the exception of the dorsal-most cells, which had little or no detectable immunoreactivity. N-CAM, but not N-cadherin, was observed on some migrating neural crest cells after their departure from the cranial neural tube. To examine the functional significance of these molecules, perturbation experiments were performed by injecting antibodies against N-CAM or N-cadherin into the cranial mesenchyme adjacent to the midbrain. Fab' fragments or whole IgGs of monoclonal and polyclonal antibodies against N-CAM caused abnormalities in the cranial neural tube and neural crest. Predominantly observed defects included neural crest cells in ectopic locations, both within and external to the neural tube, and mildly deformed neural tubes containing some dissociating cells. A monoclonal antibody against N-cadherin also disrupted cranial development, with the major defect being grossly distorted neural tubes and some ectopic neural crest cells outside of the neural tube. In contrast, nonblocking N-CAM antibodies and control IgGs had few effects. Embryos appeared to be sensitive to the N-CAM and N-cadherin antibodies for a limited developmental period from the neural fold to the 9-somite stage, with older embryos no longer displaying defects after antibody injection. These results suggest that the cell adhesion molecules N-CAM and N-cadherin are important for the normal integrity of the cranial neural tube and for the emigration of neural crest cells. Because cell-matrix interactions also are required for proper emigration of cranial neural crest cells, the results suggest that the balance between cell-cell and cell-matrix adhesion may be critical for this process.", "date": "1992-10", "date_type": "published", "publication": "Developmental Biology", "volume": "153", "number": "2", "publisher": "Elsevier", "pagerange": "291-301", "id_number": "CaltechAUTHORS:20160308-065959357", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160308-065959357", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE10066" }, { "agency": "American Cancer Society", "grant_number": "CD-416" } ] }, "doi": "10.1016/0012-1606(92)90114-V", "resource_type": "article", "pub_year": "1992", "author_list": "Bronner-Fraser, Marianne; Wolf, John J.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/66jyz-t6715", "eprint_id": 29810, "eprint_status": "archive", "datestamp": "2023-08-20 01:44:52", "lastmod": "2023-10-24 22:28:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George N." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Vital dye analysis of cranial neural crest cell migration in the mouse embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "cell migration, cranial, DiI, mouse, neural crest", "note": "\u00a9 1992 The Company of Biologists Limited. \n\nAccepted 24 June 1992.\n\nPublished - SERdev92.pdf
", "abstract": "The spatial and temporal aspects of cranial neural crest cell migration in the mouse are poorly understood because of technical limitations. No reliable cell markers are available and vital staining of embryos in culture has had limited success because they develop normally for only 24 hours. Here, we circumvent these problems by combining vital dye labelling with exo utero embryological techniques. To define better the nature of cranial neural crest cell migration in the mouse embryo, premigratory cranial neural crest cells were labelled by injecting DiI into the amniotic cavity on embryonic day 8. Embryos, allowed to develop an additional 1 to 5 days exo utero in the mother before analysis, showed distinct and characteristic patterns of cranial neural crest cell migration at the different axial levels. Neural crest cells arising at the level of the forebrain migrated ventrally in a contiguous stream through the mesenchyme between the eye and the diencephalon. In the region of the midbrain, the cells migrated ventrolaterally as dispersed cells through the mesenchyme bordered by the lateral surface of the mesencephalon and the ectoderm. At the level of the hindbrain, neural crest cells migrated ventrolaterally in three subectodermal streams that were segmentally distributed. Each stream extended from the dorsal portion of the neural tube into the distal portion of the adjacent branchial arch. The order in which cranial neural crest cells populate their derivatives was determined by labelling embryos at different stages of development. Cranial neural crest cells populated their derivatives in a ventral-to-dorsal order, similar to the pattern observed at trunk levels. In order to confirm and extend the findings obtained with exo utero embryos, DiI (1,1-dioctadecyl-3,3,3\u2032,3\u2032-tetramethylindo-carbocyanine perchlorate) was applied focally to the neural folds of embryos, which were then cultured for 24 hours. Because the culture technique permitted increased control of the timing and location of the DiI injection, it was possible to determine the duration of cranial neural crest cell emigration from the neural tube. Cranial neural crest cell emigration from the neural folds was completed by the 11-somite stage in the region of the rostral hindbrain, the 14-somite stage in the regions of the midbrain and caudal hindbrain and not until the 16-somite stage in the region of the forebrain. At each level, the time between the earliest and latest neural crest cells to emigrate from the neural tube appeared to be 9 hours.", "date": "1992-10", "date_type": "published", "publication": "Development", "volume": "116", "number": "2", "publisher": "Company of Biologists", "pagerange": "297-307", "id_number": "CaltechAUTHORS:20120322-125843475", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120322-125843475", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "SERdev92.pdf", "url": "https://authors.library.caltech.edu/records/66jyz-t6715/files/SERdev92.pdf" }, "resource_type": "article", "pub_year": "1992", "author_list": "Serbedzija, George N.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/gmzec-k8p69", "eprint_id": 65828, "eprint_status": "archive", "datestamp": "2023-08-20 01:20:17", "lastmod": "2023-10-18 16:50:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Reversal of Fate", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1992 American Association for the Advancement of Science.", "abstract": "Prior to overt differentiation when they express recognizable phenotypes, cells in developing organisms are thought to pass through a state of determination in which\nthe decision regarding their future fate is made. Although the outcome of these processes is generally assumed to be fixed, there are numerous examples in which differentiated\ncells can partially or totally switch their fate.", "date": "1992-05-15", "date_type": "published", "publication": "Science", "volume": "256", "number": "5059", "publisher": "American Association for the Advancement of Science", "pagerange": "1054-1055", "id_number": "CaltechAUTHORS:20160401-072003865", "issn": "0036-8075", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160401-072003865", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1126/science.256.5059.1054", "resource_type": "article", "pub_year": "1992", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/erdyj-a5n23", "eprint_id": 29730, "eprint_status": "archive", "datestamp": "2023-08-20 01:14:55", "lastmod": "2023-10-24 22:24:35", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-M", "name": { "family": "Artinger", "given": "Michael" } }, { "id": "Muschler-J", "name": { "family": "Muschler", "given": "John" } }, { "id": "Horwitz-A-F", "name": { "family": "Horwitz", "given": "Alan F." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Developmentally regulated expression of \u03b16 integrin in avian embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "cell surface receptors, extracellular matrix,\nnervous system, myoblasts", "note": "\u00a9 1992 The Company of Biologists Limited.\n\nAccepted 9 February 1992.\nWe thank Mary Flowers and Kristin Artinger for excellent\ntechnical assistance and Dr. Scott Fraser for helpful comments\non the manuscript. Anti-human \u03b1_5 and \u03b1_6 antiserum\nand cDN A encoding chicken \u03b1_6 were the generous gifts of Dr.\nL. F. Reichardt. This work was supported by USPHS HD-15527 to MBF and GM-23244 to AFH.\n\nPublished - BROdev92.pdf
", "abstract": "The distribution pattern of the avian \u03b1_6 integrin subunit was examined during early stages of development. The results show that this subunit is prevalent in cells of the developing nervous system and muscle. \u03b1_6 is first observed on neuroepithelial cells of the cranial neural plate and trunk neural tube. With time, immunoreactivity becomes prominent near the lumen and ventrolateral portions of the neural tube, co-distributing with neurons and axons, particularly notable on commissural neurons. The \u03b1_6 expression pattern is dynamic in the neural tube, with immunoreactivity peaking by embryonic day 6 (stage 30) and decreasing thereafter. The ventral roots and retina exhibit high levels of immunoreactivity throughout development. In the peripheral nervous system, \u03b1_6 immunoreactivity first appears on a subpopulation of sympathoadrenal cells around the dorsal aorta and later in the dorsal root ganglia shortly after gangliogenesis. Immunoreactivity appears on prospective myotomal cells as the somites delaminate into the dermomyotome and sclerotome, remaining prominent on myoblasts and differentiated muscle at all stages. The mesonephros also has intense immunoreactivity. In the periphery, \u03b1_6 immunoreactive regions often in proximity to laminin, which is thought to be the ligand of \u03b1_6\u03b2_1 integrin.", "date": "1992-05", "date_type": "published", "publication": "Development", "volume": "115", "number": "1", "publisher": "Company of Biologists", "pagerange": "197-211", "id_number": "CaltechAUTHORS:20120315-111416031", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120315-111416031", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U. S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "U. S. Public Health Service (USPHS)", "grant_number": "GM-23244" } ] }, "primary_object": { "basename": "BROdev92.pdf", "url": "https://authors.library.caltech.edu/records/erdyj-a5n23/files/BROdev92.pdf" }, "resource_type": "article", "pub_year": "1992", "author_list": "Artinger, Michael; Muschler, John; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mn6v7-tyk91", "eprint_id": 65930, "eprint_status": "archive", "datestamp": "2023-08-22 08:49:36", "lastmod": "2023-10-18 16:56:04", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Akitaya-Tatsuo", "name": { "family": "Akitaya", "given": "Tatsuo" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Expression of Cell Adhesion Molecules During Initiation and Cessation of Neural Crest Cell Migration", "ispublished": "pub", "full_text_status": "restricted", "keywords": "N-cadherin; N-CAM; Emigration; Gangliogenesis; Neural tube", "note": "\u00a9 1992 Wiley-Liss, Inc. \n\nReceived April 27, 1992; accepted April 27, 1992. \n\nWe thank Drs. Masatoshi Takeichi, Jack Lillien, David Gottlieb, and Virginia Lee for kindly providing the antibodies used in this study, Brad Martinsen for excellent technical assistance, and Drs. Thomas Lallier and Mark Selleck for helpful comments on the manuscript. This work was supported by USPHS DE10066 to M.B.F.", "abstract": "Because of their distribution and known ability to promote neuronal adhesion, it has been proposed that N-CAM and N-cadherin are involved in the formation of the nervous system. Here, we examine the expression of these molecules during the initiation and cessation of trunk neural crest cell migration during the formation of the peripheral nervous system. Whereas other neural tube cells express N-cadherin, the dorsal neural tube containing neural crest precursors has little or no N-cadherin immunoreactivity. In contrast, N-CAM is expressed in the dorsal neural tube and on early migrating neural crest cells, from which it gradually disappears during migration. Both N-CAM and N-cadherin are absent from neural crest cells at advanced stages of migration. As neural crest cells cease migration and condense to form dorsal root and sympathetic ganglia, N-cadherin but not N-CAM is observed on the forming ganglia, identified by neurofilament expression and the aggregation of HNK-1 reactive cells. The results demonstrate that the absence of N-cadherin correlates with the onset of neural crest migration and its reappearance correlates with cessation of migration and precedes ganglio-genesis.", "date": "1992-05", "date_type": "published", "publication": "Developmental Dynamics", "volume": "194", "number": "1", "publisher": "Wiley-Liss, Inc.", "pagerange": "12-20", "id_number": "CaltechAUTHORS:20160405-112342750", "issn": "1058-8388", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160405-112342750", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "DE10066" } ] }, "doi": "10.1002/aja.1001940103", "resource_type": "article", "pub_year": "1992", "author_list": "Akitaya, Tatsuo and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vg42p-e6550", "eprint_id": 65149, "eprint_status": "archive", "datestamp": "2023-08-20 00:57:00", "lastmod": "2023-10-17 23:13:54", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Partial Restriction in the Developmental Potential of Late Emigrating Avian Neural Crest Cells", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1992 Academic Press, Inc. \n\nAccepted 2 October 1991. \n\nWe thank Dr. Thomas Lallier for help with preparation of schematic figure, Mary Flowers and Michael Artinger for excellent technical assistance, and Drs. Scott Fraser and Thomas Lallier for helpful comments on the manuscript. This work was supported by USPHS HD-25138.", "abstract": "Trunk neural crest cells migrate along two major pathways: a ventral pathway through the somites whose cells form neuronal derivatives and a dorsolateral pathway underneath the ectoderm whose cells become pigmented. In avian embryos, the latest emigrating neural crest cells move only along the dorsolateral pathway. To test whether late emigrating neural crest cells are more restricted in developmental potential than early migrating cells, cultures were prepared from the neural tubes of embryos at various stages of neural crest cell migration. \"Early\" and \"middle\" aged neural crest cells differentiated into many derivatives including pigmented cells, neurofilament-immunoreactive cells, and adrenergic cells. In contrast, \"late\" neural crest cells differentiated into pigment cells and neurofilament-immunoreactive cells, but not into adrenergic cells even after 10\u201314 days. To further challenge the developmental potential of early and late emigrating neural crest cells, they were transplanted into embryos during the early phases of neural crest cell migration, known to be permissive for adrenergic neuronal differentiation. The cells were labeled with the vital dye, DiI, and injected onto the ventral pathway at stages 14\u201317. Two and three days after injection, some early neural crest cells were found to express catecholamines, suggesting they were adrenergic neuroblasts. In contrast, DiI-labeled late neural crest cells never became catecholamine-positive. These results suggest that the late emigrating neural crest cell population has a more restricted developmental potential than the early migrating neural crest cell population.", "date": "1992-01", "date_type": "published", "publication": "Developmental Biology", "volume": "149", "number": "1", "publisher": "Elsevier", "pagerange": "149-157", "id_number": "CaltechAUTHORS:20160307-150634696", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160307-150634696", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" } ] }, "doi": "10.1016/0012-1606(92)90271-H", "resource_type": "article", "pub_year": "1992", "author_list": "Artinger, Kristin B. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/sjvee-51x75", "eprint_id": 30194, "eprint_status": "archive", "datestamp": "2023-08-20 00:35:31", "lastmod": "2023-10-17 15:32:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Avian neural crest cell attachment to laminin: involvement of divalent cation dependent and independent integrins", "ispublished": "pub", "full_text_status": "public", "keywords": "extracellular matrix receptors, cell adhesion, laminin, integrin, neural crest, quail", "note": "\u00a9 1991 Company of Biologists Limited. \n\nAccepted 28 August 1991. \n\nWe thank Drs Scott Fraser and Roberto Perris for helpful\ncomments on the manuscript and invaluable discussion. We\nalso would like to thank Dr Mats Paulsson for his generous\ngift of laminin fragments and the laminin-nidogen complex.\nThis study was supported by USPHS Grant HD-15527.\n\nPublished - LALdev91.pdf
", "abstract": "The mechanisms of neural crest cell interaction with laminin were explored using a quantitative cell attachment assay. With increasing substratum concentrations, an increasing percentage of neural crest cells adhere to laminin. Cell adhesion at all substratum concentrations was inhibited by the CSAT antibody, which recognizes the chick \u03b2_1 subunit of integrin, suggesting that \u03b2_(1-)integrins mediate neural crest cell interactions with laminin. The HNK-1 antibody, which recognizes a carbohydrate epitope, inhibited neural crest cell attachment to laminin at low coating concentrations (>1 \u00b5g ml^(-1); Low-LM), but not at high coating concentration of laminin (10 \u00b5g ml^(-1); High-LM). Attachment to Low-LM occurred in the absence of divalent cations, whereas attachment to High-LM required >0.1 mM Ca^(2+) or Mn^(2+). Neural crest cell adherence to the E8 fragment of laminin, derived from its long arm, was similar to that on intact laminin at high and low coating concentrations, suggesting that this fragment contains the neural crest cell binding site(s). The HNK-1 antibody recognizes a protein of 165,000 Mr which is also found in immunoprecipitates using antibodies against the \u03b2_1 subunit of integrin and is likely to be an integrin alpha subunit or an integrin-associated protein. Our results suggest that the HNK-1 epitope on neural crest cells is present on or associated with a novel or differentially glycosylated form of \u03b2_(1-)integrin, which recognizes laminin in the apparent absence of divalent cations. We conclude that neural crest cells have at least two functionally independent means of attachment to laminin which are revealed at different substratum concentrations and/or conformations of laminin.", "date": "1991-12", "date_type": "published", "publication": "Development", "volume": "113", "number": "4", "publisher": "Company of Biologists", "pagerange": "1069-1084", "id_number": "CaltechAUTHORS:20120419-081816394", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120419-081816394", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" } ] }, "doi": "10.1242/dev.113.4.1069", "primary_object": { "basename": "LALdev91.pdf", "url": "https://authors.library.caltech.edu/records/sjvee-51x75/files/LALdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Lallier, Thomas and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/nb6z6-gym83", "eprint_id": 65234, "eprint_status": "archive", "datestamp": "2023-08-20 00:37:11", "lastmod": "2023-10-18 14:26:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Birth and Differentiation of Reticular Neurons in the Chick Hindbrain: Ontogeny of the First Neuronal Population", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1991 Cell Press. \n\nReceived 8 July 1991, Revised 30 August 1991. \n\nWe thank Drs. Scott Fraser, Michael Cullen, Andrew Lumsden, and Jonathan Cooke for their comments on the manuscript and Ors. Jay Angevine, JoAnn McConnell, and Arthur LaVelle for helpful suggestions regarding [^3H]thymidine autoradiography. The technical assistance of Virginia Satterfield was also much appreciated. This work was supported in part by grants HD25138 and BNS 8809454 to M. B-F. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked \"advertisement\" in accordance with 18 USC Section 1734 solely to indicate this fact.", "abstract": "To understand better early neuronal birth and differentiation in higher vertebrates, we have examined the time when presumptive neurons in the chick embryo first withdraw from the cell cycle and express neurofilaments. Hindbrain reticular neurons arise prior to the definitive streak stage of gastrulation and represent the first neuronal population to be born. The birth of hindbrain reticular neurons and spinal interneurons occurs in a rostrocaudal sequence that closely parallels regression of Hensen's node. More rostral brain stem neurons are born shortly after those in the hindbrain. Neurofilament expression in reticular neuroblasts first occurs by the 7-somite stage, followed by axon outgrowth by the 15-somite stage. When neural plate morphogenesis is inhibited, neurofilament expression occurs on schedule in neurons that are undergoing or have completed terminal mitosis. Our results suggest that the inductive cues governing the birth and initial differentiation of reticular neurons are imparted by gastrulation and early neurulation.", "date": "1991-12", "date_type": "published", "publication": "Neuron", "volume": "7", "number": "6", "publisher": "Elsevier", "pagerange": "947-963", "id_number": "CaltechAUTHORS:20160309-093421858", "issn": "0896-6273", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160309-093421858", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD25138" }, { "agency": "NSF", "grant_number": "BNS 8809454" } ] }, "doi": "10.1016/0896-6273(91)90340-6", "resource_type": "article", "pub_year": "1991", "author_list": "Sechrist, John and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/hhb2z-03392", "eprint_id": 30199, "eprint_status": "archive", "datestamp": "2023-08-20 00:30:57", "lastmod": "2023-10-17 15:32:42", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-R", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Krotoski-D", "name": { "family": "Krotoski", "given": "Danuta" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro", "ispublished": "pub", "full_text_status": "public", "keywords": "collagens, neural crest, avian embryo, cell migration", "note": "\u00a9 1991 Company of Biologists Limited. \n\nAccepted 12 August 1991. \n\nWe are grateful to Drs Peter Bruckner, Staffan Johansson, Charles Little, Mats Paulsson, Helene Sage, and Jorgen Wieslander for providing various antibodies, purified collagens and other matrix components. Drs Robert Burgeson, Thomas Lallier and Helene Sage are thanked for their valuable suggestions and critical reading of the manuscript. Michael Artinger, Kristin Artinger, Mary Flowers and Susan Tran are acknowledged for their excellent technical assistance. The work was supported by NIH grant USPHS HD-15527 and by the Muscular Dystrophy Association.\n\nPublished - PERdev91b.pdf
", "abstract": "This study examines the spatiotemporal distribution of collagen (Col) types I-V and IX during neural crest development in vivo and their ability to support neural crest cell movement in vitro. Col I, III and IV were widespread throughout the embryo, including the neural crest migratory pathways, whereas Col II, V and IX preferentially localized to regions from which migrating neural crest cells were absent. Col I-IV and IX occurred both in association with basement membranes and within interstitial matrices, whereas Col V only was detected in juxtaposition to basement membranes. Although initially distributed throughout the rostrocaudal extent of the somitic sclerotome, Col I and III rearranged to the caudal portion with progressive neural crest cell migration through the rostral portion of the sclerotome. This rearrangement does not occur in neural crest-ablated embryos, suggesting that it is a direct consequence of neural crest cell migration. The perinotochordal matrix, avoided by neural crest cells, contained a metameric Col II/IX immunoreactivity along the rostrocaudal axis which alternated with that of Col I and III. In contrast, Col IV and V were not observed in this matrix, but lined the basement membranes of the notochord and ventrolateral neural tube. To determine their functional significance for neural crest cell migration in vivo, purified collagens were tested for their ability to promote neural crest cell motility in vitro. Neural crest cell migration on isolated collagens was most pronounced on Col I and IV, whereas Col II, V and the triple-helical fragment of Col VII were unable to support cell motility. Substrata created by copolymerization of Col I and fibronectin, or Col I and laminin-nidogen, supported cell motility better than Col I alone, whereas both Col V and a cartilage-type chondroitin sulfate proteoglycan reduced cell movement on Col I. Fibronectin bound to pre-immobilized monomeric Col I, II or V had a reduced ability to support neural crest cell movement when compared to fibronectin alone. A similar reduction was seen for Col IV bound to the low density heparan sulfate proteoglycan from the EHS mouse tumor. The results demonstrate that Col I-IX are differentially distributed in the early avian embryo. During neural crest development several of these collagens undergo dynamic reorganizations that correlate with the migration of neural crest cells. Furthermore, various collagens possess distinct abilities to support neural crest cell migration in vitro, and their migration-promoting activity can be modulated by their conformation and/or association with other matrix components.", "date": "1991-11", "date_type": "published", "publication": "Development", "volume": "113", "number": "3", "publisher": "Company of Biologists", "pagerange": "969-984", "id_number": "CaltechAUTHORS:20120419-110343819", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120419-110343819", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" } ] }, "primary_object": { "basename": "PERdev91b.pdf", "url": "https://authors.library.caltech.edu/records/hhb2z-03392/files/PERdev91b.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Perris, Roberto; Krotoski, Danuta; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/mm4ra-xm343", "eprint_id": 30179, "eprint_status": "archive", "datestamp": "2023-08-22 08:19:45", "lastmod": "2023-10-17 15:32:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stern-C-D", "name": { "family": "Stern", "given": "Claudio D." } }, { "id": "Artinger-K-B", "name": { "family": "Artinger", "given": "Kristin B." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Tissue interactions affecting the migration and differentiation of neural crest cells in the chick embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest; chick embryo; sympathetic nervous system; catecholamines; adrenergic neurons; differentiation; segmentation", "note": "\u00a9 1991 The Company of Biologists Limited. \n\nAccepted 28 May 1991. \n\nThis work was supported by USPHS grants HD-25138 and HD-15527 and a grant from the Muscular Dystrophy Association to M. B-F. and by a grant from the Medical Research Council and a Wellcome Trust travel grant to C.D.S. M. B-F. is a Sloan Foundation Fellow.\n\nPublished - STEdev91.pdf
", "abstract": "A series of microsurgical operations was performed in chick embryos to study the factors that control the polarity, position and differentiation of the sympathetic and dorsal root ganglion cells developing from the neural crest. The neural tube, with or without the notochord, was rotated by 180 degrees dorsoventrally to cause the neural crest cells to emerge ventrally. In some embryos, the notochord was ablated, and in others a second notochord was implanted. Sympathetic differentiation was assessed by catecholamine fluorescence after aldehyde fixation. Neural crest cells emerging from an inverted neural tube migrate in a ventral-to-dorsal direction through the sclerotome, where they become segmented by being restricted to the rostral half of each sclerotome. Both motor axons and neural crest cells avoid the notochord and the extracellular matrix that surrounds it, but motor axons appear also to be attracted to the notochord until they reach its immediate vicinity. The dorsal root ganglia always form adjacent to the neural tube and their dorsoventral orientation follows the direction of migration of the neural crest cells. Differentiation of catecholaminergic cells only occurs near the aorta/mesonephros and in addition requires the proximity of either the ventral neural tube (floor plate/ventral root region) or the notochord. Prior migration of presumptive catecholaminergic cells through the sclerotome, however, is neither required nor sufficient for their adrenergic differentiation.", "date": "1991-09", "date_type": "published", "publication": "Development", "volume": "113", "number": "1", "publisher": "Company of Biologists", "pagerange": "207-216", "id_number": "CaltechAUTHORS:20120418-145914119", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120418-145914119", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Medical Research Council (MRC)" }, { "agency": "Wellcome Trust" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "primary_object": { "basename": "STEdev91.pdf", "url": "https://authors.library.caltech.edu/records/mm4ra-xm343/files/STEdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Stern, Claudio D.; Artinger, Kristin B.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/cn5d6-ez857", "eprint_id": 11827, "eprint_status": "archive", "datestamp": "2023-09-14 16:43:54", "lastmod": "2023-10-23 20:41:45", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Migrating neural crest cells in the trunk of the avian embryo are multipotent", "ispublished": "pub", "full_text_status": "public", "keywords": "cell lineage, vital dye, microinjection, neural crest, migration, avian embryo", "note": "Copyright \u00a9 1991 by Company of Biologists. \n\n(Accepted 9 April 1991) \n\nWe thank Kristin Bruk and Mary Flowers for excellent technical assistance and Drs Andres Collazo and Jonathan Ivins for helpful comments on the manuscript. This work was supported by USPHS (HD-25138).\n\nPublished - FRAdev91.pdf
", "abstract": "Trunk neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. Previously, we demonstrated that many premigratory trunk neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. The results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after leaving the neural tube either during their migration or at their sites of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were distributed unilaterally in all embryos. Most clones were confined to a single segment, though a few contributed to sympathetic ganglia over two segments. A majority of the clones gave rise to cells in multiple neural crest derivatives. Individual migrating neural crest cells gave rise to both sensory and sympathetic neurons (neurofilament-positive), as well as cells with the morphological characteristics of Schwann cells, and other non-neuronal cells (both neurofilament-negative). Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. Our data demonstrate that migrating trunk neural crest cells can be multipotent, giving rise to cells in multiple neural crest derivatives, and contributing to both neuronal and non-neuronal elements within a given derivative. Thus, restriction of neural crest cell fate must occur relatively late in migration or at the final sites of neural crest cell localization.", "date": "1991-08", "date_type": "published", "publication": "Development", "volume": "112", "number": "4", "publisher": "Company of Biologists", "pagerange": "913-922", "id_number": "CaltechAUTHORS:FRAdev91", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:FRAdev91", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" } ] }, "primary_object": { "basename": "FRAdev91.pdf", "url": "https://authors.library.caltech.edu/records/cn5d6-ez857/files/FRAdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Fraser, Scott E. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/btcar-bp225", "eprint_id": 63292, "eprint_status": "archive", "datestamp": "2023-08-19 23:52:33", "lastmod": "2023-10-25 23:42:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" }, { "id": "Stern-C-D", "name": { "family": "Stern", "given": "C. D." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "S." }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Analysis of neural crest cell lineage and migration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1991 Wiley-Liss.", "abstract": "In this review, we describe the results of recent experiments designed to investigate various aspects of neural crest cell lineage and migration. We have analyzed the lineage of individual premigratory neural crest cells by injecting a fluorescent lineage tracer dye, lysinated fluorescein dextran, into cells within the dorsal neural tube. Individual clones contained cells that were located in very diverse sites consistent with their being sensory neurons, prepigment cells, Schwann cells, adrenergic cells, and neural tube cells. These results suggest that some neural crest cells in the trunk and cranial regions are multipotent prior to their emigration from the neural tube. The environment through which neural crest cells move influences both the pattern and direction of their migration. We have shown that the sclerotomal portion of the somites are responsible for the rostrocaudal pattern of trunk neural crest cell movement, whereas the neural tube appears to govern the dorsoventral position of neural crest-derived ganglia. In addition, the notochord inhibits the movement of neural crest cells. In order to understand necessary cell-matrix interactions in neural crest migration, we have performed perturbation experiments, in which antibodies directed against cell surface or extracellular matrix molecules were introduced along neural crest pathways. We find that integrins, fibronectin, laminin, and tenascin all play some role in cranial neural crest emigration. Thus, multiple factors may be involved in controlling neural crest cell migration, and different factors may be important for migration in different regions of the embryo.", "date": "1991-04", "date_type": "published", "publication": "Journal of craniofacial genetics and developmental biology", "volume": "11", "number": "4", "publisher": "Wiley-Liss", "pagerange": "214-22", "id_number": "CaltechAUTHORS:20160101-180403287", "issn": "0270-4145", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-180403287", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "article", "pub_year": "1991", "author_list": "Bronner-Fraser, M.; Stern, C. D.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bar0g-29d28", "eprint_id": 12478, "eprint_status": "archive", "datestamp": "2023-08-22 08:02:40", "lastmod": "2023-10-17 17:00:46", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cell lineage analysis of the avian neural crest", "ispublished": "pub", "full_text_status": "public", "keywords": "developmental potential, intracellular injection, determination, cell fate", "note": "\u00a9 The Company of Biologists Ltd 1991. \n\nThis work was supported by USPHS (HD-25138).\n\nPublished - BROdev91.pdf
", "abstract": "Neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. A major unanswered question concerning the neural crest is when and how the neural crest cells become determined to adopt a particular fate. We have explored the developmental potential of trunk neural crest cells in avian embryos by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells within the dorsal neural tube. We find that premigratory and emigrating neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. These results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after emigration from the neural tube either during their migration or at their sites of localization. To determine whether neural crest cells become restricted during their migration, we have microinjected individual trunk neural crest cells with dye shortly after they leave the neural tube or as they migrate through the somite. We find that a majority of the clones derived from migrating neural crest cells appear to be multipotent; individual migrating neural crest cells gave rise to both sensory and sympathetic neurons, as well as cells with the morphological characteristics of Schwann cells, and other nonneuronal cells. Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. These data demonstrate that migrating trunk neural crest cells, like their premigratory progenitors, can be multipotent. They give rise to cells in multiple neural crest derivatives and contribute to both neuronal and non-neuronal elements within a given derivative. Thus, restriction of neural crest cell fate must occur relatively late in migration or at the final destinations.", "date": "1991-04", "date_type": "published", "publication": "Development", "volume": "113", "number": "S2", "publisher": "Company of Biologists", "pagerange": "17-22", "id_number": "CaltechAUTHORS:BROdev91", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:BROdev91", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" } ] }, "collection": "CaltechAUTHORS", "primary_object": { "basename": "BROdev91.pdf", "url": "https://authors.library.caltech.edu/records/bar0g-29d28/files/BROdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Fraser, Scott E. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/32st0-cyv14", "eprint_id": 30128, "eprint_status": "archive", "datestamp": "2023-08-19 23:51:05", "lastmod": "2023-10-17 15:23:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George N." } }, { "id": "Burgan-S", "name": { "family": "Burgan", "given": "Scott" } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "cell migration, gut, Dil, chick, mouse, enteric\nnervous system, neural crest", "note": "\u00a9 1991 The Company of Biologists Limited.\n\nAccepted 15 January 1991.\n\nPublished - SERdev91.pdf
", "abstract": "We have used the vital dye, DiI, to analyze the contribution of sacral neural crest cells to the enteric nervous system in chick and mouse embryos. In order to label premigratory sacral neural crest cells selectively, DiI was injected into the lumen of the neural tube at the level of the hindlimb. In chick embryos, DiI injections made prior to stage 19 resulted in labelled cells in the gut, which had emerged from the neural tube adjacent to somites 29\u201337. In mouse embryos, neural crest cells emigrated from the sacral neural tube between E9 and E9.5. In both chick and mouse embryos, DiI-labelled cells were observed in the rostral half of the somitic sclerotome, around the dorsal aorta, in the mesentery surrounding the gut, as well as within the epithelium of the gut. Mouse embryos, however, contained consistently fewer labelled cells than chick embryos. DiI-labelled cells first were observed in the rostral and dorsal portion of the gut. Paralleling the maturation of the embryo, there was a rostral-to-caudal sequence in which neural crest cells populated the gut at the sacral level. In addition, neural crest cells appeared within the gut in a dorsal-to-ventral sequence, suggesting that the cells entered the gut dorsally and moved progressively ventrally. The present results resolve a long-standing discrepancy in the literature by demonstrating that sacral neural crest cells in both the chick and mouse contribute to the enteric nervous system in the postumbilical gut.", "date": "1991-04", "date_type": "published", "publication": "Development", "volume": "111", "number": "4", "publisher": "Company of Biologists", "pagerange": "857-866", "id_number": "CaltechAUTHORS:20120417-095608741", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120417-095608741", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "primary_object": { "basename": "SERdev91.pdf", "url": "https://authors.library.caltech.edu/records/32st0-cyv14/files/SERdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Serbedzija, George N.; Burgan, Scott; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qxvhy-dwp34", "eprint_id": 65138, "eprint_status": "archive", "datestamp": "2023-08-19 23:43:12", "lastmod": "2023-10-17 23:13:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Stern-C-D", "name": { "family": "Stern", "given": "Claudio" } } ] }, "title": "Effects of Mesodermal Tissues on Avian Neural Crest Cell Migration", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1991 Academic Press, Inc. \n\nAccepted 9 October 1990. \n\nThis study was funded by USPHS Grant HD 15527 and a grant from the Muscular Dystrophy Association to M.B.F. and by a travel grant from the Wellcome Trust to C.D.S. M.B.F. is a Sloan Research Fellow.", "abstract": "We have used microsurgical techniques to investigate the effects of embryonic mesodermal tissues on the pattern of chick neural crest cell migration in the trunk. Segmental plate or lateral plate mesenchyme was transplanted into regions encountered by neural crest cells. We found that neural crest cells are able to migrate through lateral plate mesenchyme but not through segmental plate tissue until this tissue differentiates into a sclerotome. After this stage, segmental migration is controlled by the subdivision of the sclerotome into a rostral and a caudal half: when the rostrocaudal orientation of the sclerotomes is reversed by rotating the segmental plate 180\u00b0 about its rostrocaudal axis, neural crest cells migrate through the portion of the sclerotome that was originally rostral.", "date": "1991-02", "date_type": "published", "publication": "Developmental Biology", "volume": "143", "number": "2", "publisher": "Elsevier", "pagerange": "213-217", "id_number": "CaltechAUTHORS:20160307-141030790", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160307-141030790", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Wellcome Trust" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(91)90071-A", "resource_type": "article", "pub_year": "1991", "author_list": "Bronner-Fraser, Marianne and Stern, Claudio" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/k3sc2-nqz05", "eprint_id": 30198, "eprint_status": "archive", "datestamp": "2023-08-19 23:42:24", "lastmod": "2023-10-17 15:32:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-Roberto", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Krotoski-Danuta", "name": { "family": "Krotoski", "given": "Danuta" } }, { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Domingo-Carmen", "name": { "family": "Domingo", "given": "Carmen" } }, { "id": "Sorrell-Michael", "name": { "family": "Sorrell", "given": "Michael" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Spatial and temporal changes in the distribution of proteoglycans during avian neural crest development", "ispublished": "pub", "full_text_status": "public", "keywords": "proteoglycans, hyaluronan, neural crest, avian embryo, cell migration, extracellular matrix", "note": "\u00a9 1991 Company of Biologists Limited. \n\nAccepted 23 November 1990. \n\nWe thank Drs Bruce Caterson and Douglas Fambrough for providing various antibodies, and Dr Toward Laurent for donation of the purified proteoglycan fragment. Kristie Bruk, Mary Flowers, and Michael Artinger are thanked for their excellent technical assistance. This study was supported by NIH grant USPHS HD-15527 and the Muscular Dystrophy Association. MB-F is a Sloan Research Fellow.\n\nPublished - PERdev91a.pdf
", "abstract": "In this study, we describe the distribution of various classes of proteoglycans and their potential matrix ligand, hyaluronan, during neural crest development in the trunk region of the chicken embryo. Different types of chondroitin and keratan sulfate proteoglycans were recognized using a panel of monoclonal antibodies produced against specific epitopes on their glycosaminoglycan chains. A heparan sulfate proteoglycan was identified by an antibody against its core protein. The distribution of hyaluronan was mapped using a biotinylated fragment that corresponds to the hyaluronan-binding region of cartilage proteoglycans. Four major patterns of proteoglycan immunoreactivity were observed. (1) Chondroitin-6-sulfate-rich proteoglycans and certain keratin sulfate proteoglycans were absent from regions containing migrating neural crest cells, but were present in interstitial matrices and basement membranes along prospective migratory pathways such as the ventral portion of the sclerotome. Although initially distributed uniformly along the rostrocaudal extent of the sclerotome, these proteoglycans became rearranged to the caudal portion of the sclerotome with progressive migration of neural crest cells through the rostral sclerotome and their aggregation into peripheral ganglia. (2) A subset of chondroitin/keratan sulfate proteoglycans bearing primarily unsulfated chondroitin chains was observed exclusively in regions where neural crest cells were absent or delayed from entering, such as the perinotochordal and subepidermal spaces. (3) A subset of chondroitin/keratan sulfate proteoglycans was restricted to the perinotochordal region and, following gangliogenesis, was arranged in a metameric pattern corresponding to the sites where presumptive vertebral arches form. (4) Certain keratan sulfate proteoglycans and a heparan sulfate proteoglycan were observed in basement membranes and in an interstitial matrix uniformly distributed along the rostrocaudal extent of the sclerotome. After gangliogenesis, the neural crest-derived dorsal root and sympathetic ganglia contained both these proteoglycan types, but were essentially free of other chondroitin/keratan-proteoglycan subsets. Hyaluronan generally colocalized with the first set of proteoglycans, but also was concentrated around migrating neural crest cells and was reduced in neural crest-derived ganglia. These observations demonstrate that proteoglycans have diverse and dynamic distributions during times of neural crest development and chondrogenesis of the presumptive vertebrae. In general, chondroitin/keratan sulfate proteoglycans are abundant in regions where neural crest cells are absent, and their segmental distribution inversely correlates with that of neural crest-derived ganglia.", "date": "1991-02", "date_type": "published", "publication": "Development", "volume": "111", "number": "2", "publisher": "Company of Biologists", "pagerange": "583-599", "id_number": "CaltechAUTHORS:20120419-110230403", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120419-110230403", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1242/dev.111.2.583", "primary_object": { "basename": "PERdev91a.pdf", "url": "https://authors.library.caltech.edu/records/k3sc2-nqz05/files/PERdev91a.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Perris, Roberto; Krotoski, Danuta; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w53fv-qp454", "eprint_id": 30178, "eprint_status": "archive", "datestamp": "2023-08-22 07:57:35", "lastmod": "2023-10-17 15:32:00", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Ranscht-B", "name": { "family": "Ranscht", "given": "Barbara" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "T-cadherin expression alternates with migrating neural crest cells in the trunk of the avian embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "trunk neural crest cell migration; cell adhesion molecules; chick embryos; somite; sclerotome", "note": "\u00a9 1991 The Company of Biologists Limited.\n\nAccepted 28 September 1990.\n\nWe thank Drs Andrew Lumsden and Scott Fraser for\ncritical reading of the manuscript, and Kristin Bruk and Mary Flowers for excellent technical assistance. This work was supported by HD15527 and a Grant from the Muscular\nDystrophy Association to M.B.-F. and by HD 25938 and Basil\nO'Connor Starter Scholar Research Award 5-752 from the\nMarch of Dimes Birth Defects Foundation to B.R. M.B.-F. is\na Sloan Foundation Fellow and B.R. is a McKnight Scholar.\n\nPublished - RANdev91.pdf
", "abstract": "Trunk neural crest cells and motor axons move in a segmental fashion through the rostral (anterior) half of each somitic sclerotome, avoiding the caudal (posterior) half. This metameric migration pattern is thought to be caused by molecular differences between the rostral and caudal portions of the somite. Here, we describe the distribution of T-cadherin (truncated-cadherin) during trunk neural crest cell migration. T-cadherin, a novel member of the cadherin family of cell adhesion molecules was selectively expressed in the caudal half of each sclerotome at all times examined. T-cadherin immunostaining appeared graded along the rostrocaudal axis, with increasing levels of reactivity in the caudal halves of progressively more mature (rostral) somites. The earliest T-cadherin expression was detected in a small population of cells in the caudal portion of the somite three segments rostral to last-formed somite. This initial T-cadherin expression was observed concomitant with the invasion of the first neural crest cells into the rostral portion of the same somite in stage 16 embryos. When neural crest cells were ablated surgically prior to their emigration from the neural tube, the pattern of T-cadherin immunoreactivity was unchanged compared to unoperated embryos, suggesting that the metameric T-cadherin distribution occurs independent of neural crest cell signals. This expression pattern is consistent with the possibility that T-cadherin plays a role in influencing the pattern of neural crest cell migration and in maintaining somite polarity.", "date": "1991-01-01", "date_type": "published", "publication": "Development", "volume": "111", "number": "1", "publisher": "Company of Biologists", "pagerange": "15-22", "id_number": "CaltechAUTHORS:20120418-145852839", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120418-145852839", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-25938" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "5-752" }, { "agency": "Alfred P. Sloan Foundation" }, { "agency": "McKnight Foundation" } ] }, "primary_object": { "basename": "RANdev91.pdf", "url": "https://authors.library.caltech.edu/records/w53fv-qp454/files/RANdev91.pdf" }, "resource_type": "article", "pub_year": "1991", "author_list": "Ranscht, Barbara and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pxhh8-7g070", "eprint_id": 65141, "eprint_status": "archive", "datestamp": "2023-08-19 23:20:47", "lastmod": "2023-10-17 23:13:30", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Pettway-Unno-Zo\u00e9", "name": { "family": "Pettway", "given": "Zo\u00e9" }, "orcid": "0000-0002-5176-185X" }, { "id": "Guillory-Georgia", "name": { "family": "Guillory", "given": "Georgia" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Absence of Neural Crest Cells from the Region Surrounding Implanted Notochords in Situ", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1990 Academic Press, Inc. \n\nAccepted 10 August 1990.\n\nWe thank Dr. Scott Fraser for critical reading of the manuscript and Kristin Bruk and Mary Flowers for excellent technical assistance. This work was supported by USPHS Grant HD-15527 and a grant from the Muscular Dystrophy Association. M.B.-F. is a Sloan Foundation Fellow.", "abstract": "Avian neural crest cells migrating along the trunk ventral pathway are distributed throughout the rostral half of the sclerotome with the exception of a neural crest cell-free space of approximately 85 \u03bcm width surrounding the notochord. To determine if this neural crest cell-free space results from the notochord inhibiting neural crest cell migration, a length of quail notochord was implanted lateral to the neural tube along the neural crest ventral migratory pathway of 2-day chicken embryos. The subsequent distribution of neural crest cells was analyzed in embryos fixed 2 days after grafting. When the donor notochord was isolated using collagenase, neural crest cells avoided the ectopic notochord and were absent from the area immediately surrounding the implant (mean distance of 43 \u03bcm). The neural crest cell-free space was significantly less when notochords were isolated using trypsin or chondroitinase digestion and was completely eliminated when notochords were fixed with paraformaldehyde or methanol prior to implantation. The implanted notochords did not appear to affect the overall number of neural crest cells, and therefore were unlikely to exert this effect by altering their viability. These results suggest that the notochord produces a substance that can inhibit neural crest cell migration and that this substance is trypsin and chondroitinase labile.", "date": "1990-12", "date_type": "published", "publication": "Developmental Biology", "volume": "142", "number": "2", "publisher": "Elsevier", "pagerange": "335-345", "id_number": "CaltechAUTHORS:20160307-141031592", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031592", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(90)90354-L", "resource_type": "article", "pub_year": "1990", "author_list": "Pettway, Zo\u00e9; Guillory, Georgia; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vkn9c-81m14", "eprint_id": 65926, "eprint_status": "archive", "datestamp": "2023-08-19 22:37:33", "lastmod": "2023-10-18 16:55:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Experimental Analyses of the Migration and Cell Lineage of Avian Neural Crest Cells", "ispublished": "pub", "full_text_status": "restricted", "keywords": "neural crest cell, cell migration, cell differentiation, vita dye, extracellular matrix", "note": "\u00a9 1990 American Cleft Palate Association. \n\nParts of this work were supported by USPHS HD-15527, HD25l38, a grant from the Muscular Dystrophy Association, and a Basic Research Grant from the March of Dimes Birth Defects Association.", "abstract": "Neural crest cells migrate extensively during embryonic development and give rise to numerous and varied derivatives. Two important and unresolved questions are: what controls the migration and differentiation of these cells? This review summarizes recent experiments that address these issues. Specifically, this overview describes the pathways of neural crest cell migration, the functional importance of interactions between neural crest cells and the extracellular matrix for their movement, and studies on neural crest cell lineage in vivo by labelling individual precursor cells.", "date": "1990-04", "date_type": "published", "publication": "Cleft Palate Journal", "volume": "27", "number": "2", "publisher": "American Cleft Palate-Craniofacial Association", "pagerange": "110-120", "id_number": "CaltechAUTHORS:20160405-102021748", "issn": "1055-6656", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160405-102021748", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "March of Dimes Birth Defects Association" } ] }, "doi": "10.1597/1545-1569(1990)027%3C0110:eaotma%3E2.3.co;2", "resource_type": "article", "pub_year": "1990", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/wafsk-etb72", "eprint_id": 30275, "eprint_status": "archive", "datestamp": "2023-08-19 22:36:12", "lastmod": "2023-10-17 15:36:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George N." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1990 The Company of Biologists Limited. \n\nAccepted 5 January 1990. \n\nWe thank Scott Burgan for excellent technical assistance\nand Mark Cooper for suggesting the use of the sucrose\nsolution. This work was supported by USPHS Grant HD-25138 and the March of Dimes Birth Defects Foundation.\n\nPublished - SERdev90.pdf
", "abstract": "Analysis of neural crest cell migration in the mouse has been difficult due to the lack of reliable cell markers. Recently, we found that injection of DiI into the chick neural tube marks premigratory neural crest cells whose endfeet are in contact with the lumen of the neural tube (Serbedzija et al. Development 106, 809\u2013819 (1989)). In the present study, this technique was applied to study neural crest cell migratory pathways in the trunk of the mouse embryo. Embryos were removed from the mother between the 8th and the 10th days of development and DiI was injected into the lumen of the neural tube. The embryos were then cultured for 12 to 24 h, and analyzed at the level of the forelimb. We observed two predominant pathways of neural crest cell migration: (1) a ventral pathway through the rostral portion of the somite and (2) a dorsolateral pathway between the dermamyotome and the epidermis. Neural crest cells were observed along the dorsolateral pathway throughout the period of migration. The distribution of labelled cells along the ventral pathway suggested that there were two overlapping phases of migration. An early ventrolateral phase began before E9 and ended by E9.5; this pathway consisted of a stream of cells within the rostral sclerotome, adjacent to the dermamyotome, that extended ventrally to the region of the sympathetic ganglia and the dorsal aorta.", "date": "1990-04", "date_type": "published", "publication": "Development", "volume": "108", "number": "4", "publisher": "Company of Biologists", "pagerange": "605-612", "id_number": "CaltechAUTHORS:20120424-102930429", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120424-102930429", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "primary_object": { "basename": "SERdev90.pdf", "url": "https://authors.library.caltech.edu/records/wafsk-etb72/files/SERdev90.pdf" }, "resource_type": "article", "pub_year": "1990", "author_list": "Serbedzija, George N.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fnfzc-c6830", "eprint_id": 65142, "eprint_status": "archive", "datestamp": "2023-08-19 22:27:16", "lastmod": "2023-10-17 23:13:34", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Leblanc-G-G", "name": { "family": "Leblanc", "given": "Gabrielle G." } }, { "id": "Epstein-M-L", "name": { "family": "Epstein", "given": "Miles L." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Differential Development of Cholinergic Neurons from Cranial and Trunk Neural Crest Cells in Vitro", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1990 Academic Press, Inc. Accepted 5 October 1989, Available online 4 November 2004.\n\nWe thank Gregory Rafijah and Adela Augsberger for their excellent technical assistance. This work was supported by National Science Foundation Grant ENS 8702512 and a grant-in-aid from the American Heart Association, California Affiliate.", "abstract": "Several studies have suggested that the development of cholinergic properties in cranial parasympathetic neurons is determined by these cells' axial level of origin in the neural crest. All cranial parasympathetic neurons normally derive from cranial neural crest. Trunk neural crest cells give rise to sympathetic neurons, most of which are noradrenergic. To determine if there is an intrinsic difference in the ability of cranial and trunk neural crest cells to form cholinergic neurons, we have compared the development of choline acetyltransferase (ChAT)-immunoreactive cells in explants of quail cranial and trunk neural crest in vitro. Both cranial and trunk neural crest explants gave rise to ChAT-immunoreactive cells in vitro. In both types of cultures, some of the ChAT-positive cells also expressed immunoreactivity for the catecholamine synthetic enzyme tyrosine hydroxylase. However, several differences were seen between cranial and trunk cultures. First, ChAT-immunoreactive cells appeared two days earlier in cranial than in trunk cultures. Second, cranial cultures contained a higher proportion of ChAT-immunoreactive cells. Finally, a subpopulation of the ChAT-immunoreactive cells in cranial cultures exhibited neuronal traits, including neurofilament immunoreactivity. In contrast, neurofilament-immunoreactive cells were not seen in trunk cultures. These results suggest that premigratory cranial and trunk neural crest cells differ in their ability to form cholinergic neurons.", "date": "1990-02", "date_type": "published", "publication": "Developmental Biology", "volume": "137", "number": "2", "publisher": "Elsevier", "pagerange": "318-330", "id_number": "CaltechAUTHORS:20160307-141031892", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031892", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF", "grant_number": "ENS 8702512" }, { "agency": "American Heart Association, California Affiliate" } ] }, "doi": "10.1016/0012-1606(90)90257-J", "resource_type": "article", "pub_year": "1990", "author_list": "Leblanc, Gabrielle G.; Epstein, Miles L.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b6fjk-w6t28", "eprint_id": 63291, "eprint_status": "archive", "datestamp": "2023-08-19 22:27:08", "lastmod": "2023-10-25 23:42:26", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krotoski-D", "name": { "family": "Krotoski", "given": "Danuta" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Distribution of integrins and their ligands in the trunk of Xenopus laevis during neural crest cell migration", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1990 Wiley-Liss. \n\nReceived April 4, 1989; accepted July 20, 1989. \n\nWe thank Drs. Roberto Perris and Scott Fraser for critical reading of the manuscript, Michael Artinger for technical assistance, and Thomas Lallier for help with immunoblots. This work was supported by USPHS grant HD15527 and NSF grant BNS 8607760.", "abstract": "We have examined the distribution in Xenopus embryos of \u03b21 subunits of integrin, as recognized by cross-reactive antibodies against the avian integrin beta1 subunit. These antibodies recognize a doublet of bands of approximately 120 kD in Xenopus embryos. The distribution pattern of these integrin cell surface receptors was compared with that of two possible ligands, fibronectin and laminin, in the extracellular matrix during the time of neural crest cell migration. Integrin immunoreactivity in the early neurula was observed lightly outlining somite and epidermal cells and the notochord. The integrin immunostaining increased with developmental age and was observed on most cell types in the embryo but was particularly notable in the intersomitic clefts through which motoraxons grow. The immunoreactivity in this region was not, however, wholly on the axon surfaces, since intersomitic integrin remained detectable in embryos in which the neural tube had been ablated. Fibronectin and laminin were more extensively distributed than integrin at all stages examined. Immunoreactivity for both was observed around the neural tube, notochord, somites, epidermis, dorsal mesentery, and lateral plate mesoderm. The distribution of laminin and fibronectin around the somites was particularly interesting since it was non-uniform and similar to that of integrin. Strongest staining was observed in the intersomitic clefts, and weakest staining was observed on the medial surface of the somites, which faces the neural tube and notochord. The major differences in distribution pattern between the fibronectin and laminin immunoreactivities were that only fibronectin was detected in the mesenchyme of the dorsal fin. Our results demonstrate that a molecule homologous to avian integrin is present in Xenopus embryos during neural crest cell migration and motoraxon outgrowth. Its presence in the intersomitic clefts and on the surface of many embryonic cell types together with the abundant distribution of its ligands are consistent with a potentially important developmental function in neurite outgrowth and/or muscle development.", "date": "1990-02", "date_type": "published", "publication": "Journal of Experimental Zoology", "volume": "253", "number": "2", "publisher": "Wiley-Liss", "pagerange": "139-150", "id_number": "CaltechAUTHORS:20160101-174449164", "issn": "0022-104X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-174449164", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "NSF", "grant_number": "BNS 8607760" } ] }, "doi": "10.1002/jez.1402530204", "resource_type": "article", "pub_year": "1990", "author_list": "Krotoski, Danuta and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/d9p5g-dnp40", "eprint_id": 65171, "eprint_status": "archive", "datestamp": "2023-08-19 22:15:43", "lastmod": "2023-10-17 23:14:59", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coulombe-J", "name": { "family": "Coulombe", "given": "J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Development of Cholinergic Traits in the Quail Ciliary Ganglion: Expression of Choline Acetyltransferase-like Immunoreactivity", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1990 Pergamon Press Inc. \n\nAccepted 27 November 1989. \n\nWe thank Dr Gabrielle Leblanc for her critical reading of the manuscript and helpful suggestions. We are also indebted to Drs Miles Epstein and Paul Salvaterra for generous gifts of antibody and purified choline acetyltransferase. This work was supported by DCB 85-02604 and BNS 86-07760. M.B.F. is a Sloan Foundation Fellow.", "abstract": "The avian ciliary ganglion is a parasympathetic ganglion derived from the neurol crest whose neurons provide cholinergic innervation to the eye. Here, we describe the time course of appearance and the morphology of cholinergic cells in the ciliary ganglion, as assessed by antibodies against choline acetyltransferase. Choline acetyltransferase immunoreactivity was first observed in 5.5-day-old quail embryos, 1 day after condensation of the ciliary ganglion. Both the intensity of choline acetyltransferase immunoreactivity and size of the choline acetyltransferase-immunoreactive cells increased with ganglionic age. By 12 days, a second population of choline acetyltransferase-immunoreactive cells, possibly corresponding to choroid neurons, was observed whose cells were smaller and less intensely stained than earlier differentiating choline acetyltransferase-immunoreactive cells. The percentage of choline acetyltransferase-immunoreactive cells was initially high, constituting approximately 50% of the total cell population. As a function of time, the proportion of cholinergic cells decreased, probably due to proliferation of non-neuronal cells and naturally-occurring cell death.\nOur results confirm the existence of two morphologically distinct populations of cholinergic neurons in the avian ciliary ganglion and demonstrate that these neuronal subpopulations express choline acetyltransferase immunoreactivity at different times in development. Because choroid neurons innervate their targets later than ciliary neurons, this finding is consistent with the hypothesis that target interactions regulate expression of choline acetyltransferase.", "date": "1990", "date_type": "published", "publication": "Neuroscience", "volume": "37", "number": "1", "publisher": "Elsevier", "pagerange": "259-270", "id_number": "CaltechAUTHORS:20160308-072342329", "issn": "0306-4522", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160308-072342329", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF", "grant_number": "DCB 85-02604" }, { "agency": "NSF", "grant_number": "BNS 86-07760" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0306-4522(90)90212-M", "resource_type": "article", "pub_year": "1990", "author_list": "Coulombe, J. and Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/n3zah-fzt08", "eprint_id": 65188, "eprint_status": "archive", "datestamp": "2023-08-22 07:02:39", "lastmod": "2024-01-13 16:43:30", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" }, { "id": "Artinger-Michael", "name": { "family": "Artinger", "given": "Michael" } }, { "id": "Matthew-William-D", "name": { "family": "Matthew", "given": "William" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Distribution and biochemical characterization of the INO antigen during chick neural crest cell migration", "ispublished": "unpub", "full_text_status": "restricted", "keywords": "Laminin-heparan sulfate proteoglycan; Extracellular matrix; Neurite outgrowth", "note": "\u00a9 1990 Elsevier Scientific Publishers Ireland Ltd. \n\nThis work was supported by USPHS Grant HD15527 and a grant from the Muscular Dystrophy Association to MB-F. MB-F is a Sloan Foundation Fellow.", "abstract": "The INO (inhibitor of neurite outgrowth) antibody recognizes a laminin-heparan sulfate proteoglycan complex and was isolated for its ability to functionally inhibit axonal outgrowth of peripheral neurons. Here, we examine the distribution and biochemical characteristics of INO in the early chick embryo. Because the INO antigen is sensitive to most classical fixation procedures and fixation leads to abundant nuclear staining, the antibody was directly injected into 1.5\u20132.5-day-old embryos prior to fixation. The distribution of the injected antibody was then observed in cryostat sections by indirect immunofluorescence. Particular attention was focussed upon regions of ongoing neural crest cell migration. The INO antigen was observed along both cranial and trunk neural crest cell migratory pathways. The antigen was seen around the basement membrane surrounding the neural tube and notochord, and underneath the ectoderm and endoderm. In addition, fibrillar staining was observed in the cranial mesenchyme and in both rostral and caudal halves of the somitic sclerotome in the trunk. The distribution pattern was identical to that previously observed for laminin or heparan sulfate proteoglycan. To confirm the nature of the INO antigen, we performed immunoprecipitations of chick embryos ranging from 1.5 to 9 days of incubation. Half of each sample was digested with heparinase prior to SDS-PAGE and silver staining. In material from young embryos, bands of 200 and 180 kD (probably corresponding to the B-chains of laminin) plus two broad smears of bands at 180\u2212150 kD and 130\u221285 kD were observed without heparinase digestion. Following enzymatic digestion, the 200-kD and 180-kD bands remained, while the smears disappeared and were replaced by numerous low-molecular-weight bands. In contrast to preparations from young embryos, samples taken from embryos at day 3 or beyond did not enter the 8% gel without heparinase digestion, though the banding pattern appeared identical to younger samples after heparinase digestion in the presence or absence of Ca^(2+). This change in the INO antigen with age could result from an increase in the heparin-side-chains attached to similar core proteins, or from an increase in the stability of the laminin-heparan sulfate proteoglycan containing complex with time.", "date": "1990", "date_type": "published", "publisher": "Elsevier Ireland Ltd", "id_number": "CaltechAUTHORS:20160308-095624786", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160308-095624786", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "contributors": { "items": [ { "id": "Obata-Ebashi-K", "name": { "family": "Obata", "given": "Ebashi-K" } } ] }, "doi": "10.1016/0921-8696(90)90041-Z", "resource_type": "book_section", "pub_year": "1990", "author_list": "Lallier, Thomas; Artinger, Michael; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/apfrn-3e122", "eprint_id": 67086, "eprint_status": "archive", "datestamp": "2023-08-19 22:03:11", "lastmod": "2023-10-18 19:57:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott" }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Developmental potential of avian trunk neural crest cells in situ", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1989 Cell Press. \n\nReceived June 6, 1989; revised September 12, 1989. \n\nWe thank Drs. Andrew Cameron, Gabrielle Leblanc, Katy Lynch, Nancy O'Rourke, John Sechrist, Jes Stollberg, George Serbedzija, and William Todt for helpful comments on the manuscript and Mary Flowers and Scott Burgan for excellent technical assistance. This work was supported by the USPHS (HD-25138) and the Monsanto Corporation.", "abstract": "To analyze the developmental potential of individual neural crest cells or their precursors, we have microinjected a vital dye, lysinated rhodamine dextran (LRD), into single cells in the dorsal neural tube. The phenotypes of the descendants that inherited the LRD from the injected cells were evaluated based upon their position, morphology, and neurofilament expression. Individual neural crest cells labeled before or as they emigrated from the neural tube gave rise to both sensory and sympathetic neurons as well as nonneuronal cells, some of which had the morphological characteristics of Schwann cells or pigment cells. In numerous cases, the descendants of a single cell included both neural crest- and neural tube-derived neurons, suggesting that some cells of the peripheral and central nervous systems share a common lineage. Our data demonstrate definitively that both emigrating and premigratory trunk neural crest cells can be multipotent, giving rise not only to cells in multiple neural crest derivatives, but also to both neuronal and nonneuronal elements within a given derivative.", "date": "1989-12", "date_type": "published", "publication": "Neuron", "volume": "3", "number": "6", "publisher": "Elsevier", "pagerange": "755-766", "id_number": "CaltechAUTHORS:20160513-130801429", "issn": "0896-6273", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160513-130801429", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-25138" }, { "agency": "Monsanto Corporation" } ] }, "doi": "10.1016/0896-6273(89)90244-4", "resource_type": "article", "pub_year": "1989", "author_list": "Bronner-Fraser, Marianne and Fraser, Scott" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qwhch-s7m24", "eprint_id": 66188, "eprint_status": "archive", "datestamp": "2023-08-19 21:59:04", "lastmod": "2023-10-18 17:15:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Perris-R", "name": { "family": "Perris", "given": "Roberto" } }, { "id": "Paulsson-M", "name": { "family": "Paulsson", "given": "Mats" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Molecular Mechanisms of Avian Neural Crest Cell Migration on Fibronectin and Laminin", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1989 Academic Press, Inc. \n\nAccepted June 27, 1989. \n\nWe are grateful to Katrina Saladin for assistance in the preparation of laminin fragments and to Dr. Staffan Johansson for valuable suggestions and the contribution of fibronectin fragments and YIGSR peptides. We thank Dr. Hynda Kleinman for generously donating YIGSR peptides, Dr. Michael Pierschbacher for kindly providing various RGD synthetic peptides and information on fibronectin fragments,\nand Dr. Daniel Carson for the donation of PF4. This study was supported by USPHS HD-15527 and a Basic Research Grant from the March of Dimes Birth Defects Foundation (to M.B.-F.), the Swiss National Science Foundation, and the Maurice Millier Foundation (to M.P.). M.B.-F. is a Sloan Foundation Research Fellow.", "abstract": "We have examined the molecular interactions of avian neural crest cells with fibronectin and laminin in vitro during their initial migration from the neural tube. A 105-kDa proteolytic fragment of fibronectin encompassing the defined cell-binding domain (65 kDa) promoted migration of neural crest cells to the same extent as the intact molecule. Neural crest cell migration on both intact fibronectin and the 105-kDa fragment was reversibly inhibited by RGD-containing peptides. The 11.5-kDa fragment containing the RGDS cell attachment site was also able to support migration, whereas a 50-kDa fragment corresponding to the adjacent N-terminal portion of the defined cell-binding domain was unfavorable for neural crest cell movement. In addition to the putative \"cell-binding domain,\" neural crest cells were able to migrate on a 31-kDa fragment corresponding to the C-terminal heparin-binding (II) region of fibronectin, and were inhibited in their migration by exogenous heparin, but not by RGDS peptides. Heparin potentiated the inhibitory effect of RGDS peptides on intact fibronectin, but not on the 105-kDa fragment. On substrates of purified laminin, the extent of avian neural crest cell migration was maximal at relatively low substrate concentrations and was reduced at higher concentrations. The efficiency of laminin as a migratory substrate was enhanced when the glycoprotein occurred complexed with nidogen. Moreover, coupling of the laminin-nidogen complex to collagen type IV or the low density heparan sulfate proteoglycan further increased cell dispersion, whereas isolated nidogen or the proteoglycan alone were unable to stimulate migration and collagen type IV was a significantly less efficient migratory substrate than laminin-nidogen. Neural crest cell migration on laminin-nidogen was not affected by RGDS nor by YIGSR-containing peptides, but was reduced by 35% after addition of heparin. The predominant motility-promoting activity of laminin was localized to the E8 domain, possessing heparin-binding activity distinct from that of the N-terminal E3 domain. Migration on the E8 fragment was reduced by >70% after addition of heparin. The E1\u2032 fragment supported a minimal degree of migration that was RGD-sensitive and heparin-insensitive, whereas the primary heparin-binding E3 fragment and the cell-adhesive P1 fragment were entirely nonpermissive for cell movement. Preincubation of laminin-nidogen substrates with antisera against the E8 fragment, but not against the E1\u2032 or the E4 fragment, potently reduced migration on the complex, further suggesting that the E8 domain is the predominant motility-promoting region of laminin. We conclude that initial neural crest cell migration on fibronectin occurs primarily through an interaction with the RGDS site within the cell-binding domain, whereas other potential attachment/motility-promoting sites may act to stabilize cell-fibronectin linkages. Neural crest cell migration on laminin is primarily mediated by the E8 domain. The efficiency of this domain as well as the ability of other potential motility-promoting domains to stimulate cell movement may be influenced by the association of laminin with other extracellular matrix molecules.", "date": "1989-11", "date_type": "published", "publication": "Developmental Biology", "volume": "136", "number": "1", "publisher": "Elsevier", "pagerange": "222-238", "id_number": "CaltechAUTHORS:20160414-144015620", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-144015620", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes Birth Defects Foundation" }, { "agency": "Swiss National Science Foundation (SNSF)" }, { "agency": "Maurice M\u00fcller Foundation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(89)90144-9", "resource_type": "article", "pub_year": "1989", "author_list": "Perris, Roberto; Paulsson, Mats; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/qzk3x-jyj63", "eprint_id": 11602, "eprint_status": "archive", "datestamp": "2023-08-22 06:52:37", "lastmod": "2023-10-17 15:09:52", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Stern-C-D", "name": { "family": "Stern", "given": "Claudio D." } }, { "id": "Norris-W-E", "name": { "family": "Norris", "given": "Wendie E." } }, { "id": "Carlson-G-J", "name": { "family": "Carlson", "given": "Geoffrey J." } }, { "id": "Faissner-A", "name": { "family": "Faissner", "given": "Andreas" } }, { "id": "Keynes-R-J", "name": { "family": "Keynes", "given": "Roger J." } }, { "id": "Schachner-M", "name": { "family": "Schachner", "given": "Metlitta" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo", "ispublished": "pub", "full_text_status": "public", "keywords": "tenascin, cytotactin, J1, segmentation, somites, neural crest, motor axons, peripheral nervous system, substrate-adhesion molecules (SAMs)", "note": "Copyright \u00a9 1989 by Company of Biologists. \n\n(Accepted 26 June 1989) \n\nThis work was supported by a grant from Action Research for the Crippled Child to CDS. MBF, who is a Sloan Foundation Research Fellow, is supported by grants from USPHS (HD-15527), March of Dimes (1-896), and the Muscular Dystrophy Association. AF and MS are supported by the German Research Society. MS is a member of the Swiss Federal Institute of Technology, Zurich.\n\nPublished - STEdev89.pdf
", "abstract": "It has been suggested that substrate adhesion molecules of the tenascin family may be responsible for the segmented outgrowth of motor axons and neural crest cells during formation of the peripheral nervous system. We have used two monoclonal antibodies (M1B4 and 578) and an antiserum [KAF9(1)] to study the expression of J1/tenascin-related molecules within the somites of the chick embryo. Neural crest cells were identified with monoclonal antibodies HNK-1 and 20B4. Young somites are surrounded by J1/tenascin immunoreactive material, while old sclerotomes are immunoreactive predominantly in their rostral halves, as described by other authors (Tan et al. 1987--Proc. natn. Acad. Sci. U.S.A. 84, 7977; Mackie et al. 1988--Development 102, 237). At intermediate stages of development, however, immunoreactivity is found mainly in the caudal half of each sclerotome. After ablation of the neural crest, the pattern of immunoreactivity is no longer localised to the rostral halves of the older, neural-crest-free sclerotomes. SDS-polyacrylamide gel electrophoresis of affinity-purified somite tissue, extracted using M1B4 antibody, shows a characteristic set of bands, including one of about 230 x 10(3), as described for cytotactin, J1-200/220 and the monomeric form of tenascin. Affinity-purified somite material obtained from neural-crest-ablated somites reveals some of the bands seen in older control embryos, but the high molecular weight components (120-230 x 10(3] are missing. Young epithelial somites also lack the higher molecular mass components. The neural crest may therefore participate in the expression of J1/tenascin-related molecules in the chick embryo. These results suggest that these molecules are not directly responsible for the segmented outgrowth of precursors of the peripheral nervous system.", "date": "1989-10", "date_type": "published", "publication": "Development", "volume": "107", "number": "2", "publisher": "Company of Biologists", "pagerange": "309-319", "id_number": "CaltechAUTHORS:STEdev89", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:STEdev89", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Action Research for the Crippled Child" }, { "agency": "Alfred P. Sloan Foundation" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes", "grant_number": "1-896" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "Deutsche Forschungsgemeinschaft (DFG)" } ] }, "primary_object": { "basename": "STEdev89.pdf", "url": "https://authors.library.caltech.edu/records/qzk3x-jyj63/files/STEdev89.pdf" }, "resource_type": "article", "pub_year": "1989", "author_list": "Stern, Claudio D.; Norris, Wendie E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/x2qfj-jd734", "eprint_id": 31420, "eprint_status": "archive", "datestamp": "2023-08-22 06:29:09", "lastmod": "2023-10-17 16:30:34", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Serbedzija-G-N", "name": { "family": "Serbedzija", "given": "George N." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A vital dye analysis of the timing and pathways of avian trunk neural crest cell migration", "ispublished": "pub", "full_text_status": "public", "keywords": "DiI; microinjection; pigment cells; cell migration; neural crest; vital dye", "note": "\u00a9 1989 Company of Biologists Ltd.\n\nAccepted 9 May 1989.\n\nWe thank Dr Andrew Lumsden for critical reading of the\nmanuscript and Scott Burgan for excellent technical assistance. This work was supported by BNS 8608356 (SEF), HD\n25138-01, a grant from the Muscular Dystrophy Association\n(MDA), a McKnight Scholar Award, and a gift from the\nMonsanto Corporation. MBF is a Sloan Foundation Fellow.\n\nPublished - SERdev89.pdf
", "abstract": "To permit a more detailed analysis of neural crest cell migratory pathways in the chick embryo, neural crest cells were labelled with a nondeleterious membrane intercalating vital dye, DiI. All neural tube cells with endfeet in contact with the lumen, including premigratory neural crest cells, were labelled by pressure injecting a solution of DiI into the lumen of the neural tube. When assayed one to three days later, migrating neural crest cells, motor axons, and ventral root cells were the only cells types external to the neural tube labelled with DiI. During the neural crest cell migratory phase, distinctly labelled cells were found along: (1) a dorsolateral pathway, under the epidermis, as well adjacent to and intercalating through the dermamyotome; and (2) a ventral pathway, through the rostral portion of each sclerotome and around the dorsal aorta as described previously. In contrast to those cells migrating through the sclerotome, labelled cells on the dorsolateral pathway were not segmentally arranged along the rostrocaudal axis. DiI-labelled cells were observed in all truncal neural crest derivatives, including subepidermal presumptive pigment cells, dorsal root ganglia, and sympathetic ganglia. By varying the stage at which the injection was performed, neural crest cell emigration at the level of the wing bud was shown to occur from stage 13 through stage 22. In addition, neural crest cells were found to populate their derivatives in a ventral-to-dorsal order, with the latest emigrating cells migrating exclusively along the dorsolateral pathway.", "date": "1989-08", "date_type": "published", "publication": "Development", "volume": "106", "number": "4", "publisher": "Company of Biologists", "pagerange": "809-816", "id_number": "CaltechAUTHORS:20120510-141811381", "issn": "0950-1991", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120510-141811381", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF", "grant_number": "BNS 8608356" }, { "agency": "NIH", "grant_number": "HD 25138-01" }, { "agency": "Muscular Dystrophy Association" }, { "agency": "McKnight Foundation" }, { "agency": "Monsanto Corporation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "primary_object": { "basename": "SERdev89.pdf", "url": "https://authors.library.caltech.edu/records/x2qfj-jd734/files/SERdev89.pdf" }, "resource_type": "article", "pub_year": "1989", "author_list": "Serbedzija, George N.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/rd2yx-xtj86", "eprint_id": 67229, "eprint_status": "archive", "datestamp": "2023-08-19 21:12:31", "lastmod": "2023-10-18 20:58:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Sechrist-J-W", "name": { "family": "Sechrist", "given": "John" } }, { "id": "Coulombe-J-N", "name": { "family": "Coulombe", "given": "James N." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Combined Vital Dye Labelling and Catecholamine Histofluorescence of Transplanted Ciliary Ganglion Cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1989 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. \n\nWe thank Dr. Gabrielle Leblanc for a critical reading of the manuscript and Michael Artinger for his technical assistance. This work was supported by BNS 8809454.\n\nPublished - 452098.pdf
", "abstract": "We have utilized the carbocyanine dye, Dil, to label suspensions of dissociated ciliary ganglion cells removed from 6 to 12 day old quail embryos. Some of the cells were injected into the trunk somites of 2.5 - 3 day old chick embryos along pathways where neural crest cells migrate\nto form sensory and sympathetic ganglia, aortic plexuses and the adrenal medulla; the remainder of the cells were cultured to check their viability and the persistence of the Dil label. Embryos were incubated for 1 - 8 days post-injection, fixed in 4% paraformaldehyde/0.25% glutaraldehyde and processed for cryostat sectioning.\nDH-labelled cells were readily identifiable in culture and in sections of embryos at all stages examined. Several cell types were identified, based on their morphology and soma size. These included cells with large cell bodies and bright\nDil-labelling that appeared to be neurons and smaller, more weakly labelled cells that appeared non-neuronal. The latter presumably bad divided several times, accounting for their reduced levels of dye. Many of the Dil-labelled\ncells were found in and around neural crestderived sympathetic ganglia, aortic plexuses and adrenomedullary cords, but were rarely observed in dorsal root ganglia. The aldehyde fixative (Faglu mixture) used in this study reacts with catecholamines to form a bright reaction product\nin adrenergic cells including those in the sympathetic ganglia and the adrenal medulla. The catecholamine biproduct and the Dil in the same cell can easily be viewed with different fluorescent filter sets. A variable number of the DU-labelled cells in these adrenergic sites contained\ncatecholamines. Cells derived from younger 6 day ciliary ganglion dissociates exhibited detectable catecholamine neurotransmitters earlier and more frequently than those derived from 8 day embryos. The presence of cells exhibiting\nboth bright Dil and catecholamine fluorescence is consistent with previous indications that post-mitotic ciliary ganglion neurons can undergo pbenotypic conversion from cholinergic to adrenergic when transplanted to the trunk environment.", "date": "1989", "date_type": "published", "publication": "Journal of Neural Transplantation", "volume": "1", "number": "3-4", "publisher": "Hindawi Publishing Corporation", "pagerange": "113-128", "id_number": "CaltechAUTHORS:20160520-152504213", "issn": "1352-237X", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160520-152504213", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF", "grant_number": "BNS 8809454" } ] }, "doi": "10.1155/NP.1989.113", "primary_object": { "basename": "452098.pdf", "url": "https://authors.library.caltech.edu/records/rd2yx-xtj86/files/452098.pdf" }, "resource_type": "article", "pub_year": "1989", "author_list": "Sechrist, John; Coulombe, James N.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1gg0e-3ys32", "eprint_id": 63290, "eprint_status": "archive", "datestamp": "2023-08-22 06:01:55", "lastmod": "2023-10-25 23:42:23", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Distribution and function of tenascin during cranial neural crest development in the chick", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest migration; immunoreactivity; neural tube defects; myotendinous antigen", "note": "\u00a9 1988 Alan R. Liss, Inc. \n\nSpecial Issue: Neuroembryology -- Cellular and Molecular Approaches \n\nI thank Drs. Roberto Perris, Thomas Lallier, and Danuta Krotoski for helpful comments on the manuscript and Geogia Guillory, Mary Flowers, and Michael Artinger for excellent technical assistance. This work was supported by USPHS grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes. The author is a Sloan Foundation Research Fellow.", "abstract": "Tenascin is a glycoprotein associated with the extra-cellular matrix and the surface of some cell types. Here, the distribution and possible function of tenascin have been examined along the pathways followed by cranial neural crest cells. During early stages of neural crest migration, tenascin was observed in a dense matrix surrounding premigratory cranial neural crest cells. Along the neural tube, tenascin immunoreactivity was observed in a dorsoventral gradient and was also noted under the ectoderm and around the notochord. During advanced neural crest migration, tenascin immunoreactivity colocalized with and appeared to be on the surface of migrating neural crest cells. At later stages, tenascin was present around the otic vesicles, retina, lens, and in an interstitial matrix in the region of the branchial arches. At the level of the occipital somites, tenascin immunoreactivity was observed around the neural tube, notochord, dermamyotome, and on the basal surface of the ectoderm. Tenascin was also observed in an interstitial matrix within the sclerotome. At early stages of vagal neural crest migration, immunoreactivity was uniform within the sclerotome, whereas at later stages tenascin colocalized with vagal neural crest cells within the rostral half of each sclerotome. The possible function of tenascin was tested by injecting antitenascin antibodies lateral to the mesencephalic neural tube. Two predominant defects were noted in injected embryos: (1) ectopic aggregates of cranial neural crest cells external to the neural tube and sometimes located on the apical side of the ectoderm; and (2) open and deformed neural tubes. Both the distribution and results of the perturbation experiment suggest that tenascin is required for proper cranial neural crest migration.", "date": "1988-10", "date_type": "published", "publication": "Journal of Neuroscience Research", "volume": "21", "number": "2-4", "publisher": "Wiley", "pagerange": "135-147", "id_number": "CaltechAUTHORS:20160101-144022515", "issn": "0360-4012", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-144022515", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes", "grant_number": "1-896" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1002/jnr.490210206", "resource_type": "article", "pub_year": "1988", "author_list": "Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fkr7c-pyq43", "eprint_id": 67126, "eprint_status": "archive", "datestamp": "2023-08-19 20:54:39", "lastmod": "2023-10-18 20:34:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Cell lineage analysis reveals multipotency of some avian neural crest cells", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1988 Nature Publishing Group. \n\nReceived 25 May; accepted 14 July 1988. \n\nWe thank Drs Gabrielle Leblanc, Roberto Perris, Jes Stollberg, Nancy O'Rourke, Rick Wetts and George Serbedzija for helpful comments, Georgia Guillory and Carmen Domingo for technical assistance and Paige Fraser for support in the early phases of this work. This work was supported by grants from NIH, NSF, March of Dimes and a gift from the Monsanto Corporation. S.E.F. is a McKnight Foundation Scholar. M.B.-F. is a Sloan Research Fellow.", "abstract": "A major question in developmental biology is how precursor cells give rise to diverse sets of differentiated cell types. In most systems, it remains unclear whether the precursors can form many or all cell types (multipotent or totipotent), or only a single cell type (predetermined). The question of cell lineage is central to the neural crest because it gives rise to numerous and diverse derivatives including peripheral neurons, glial and Schwann cells, pigment cells, and cartilage. Although the sets of derivatives arising from different populations of neural crest cells have been well-documented, relatively little is known about the developmental potentials of individual neural crest cells. We have iontophoretically microinjected the vital dye, lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells to mark unambiguously their descendants. Many of the resulting labelled clones consisted of multiple cell types, as judged by both their location and morphology. Cells as diverse as sensory neurons, presumptive pigment cells, ganglionic supportive cells, adrenomedullary cells and neural tube cells were found within individual clones. Our results indicate that at least some neural crest cells are multipotent before their departure from the neural tube.", "date": "1988-09-08", "date_type": "published", "publication": "Nature", "volume": "335", "number": "6186", "publisher": "Nature Publishing Group", "pagerange": "161-164", "id_number": "CaltechAUTHORS:20160516-110121358", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160516-110121358", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "NSF" }, { "agency": "March of Dimes Foundation" }, { "agency": "Monsanto Corporation" }, { "agency": "McKnight Foundation" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1038/335161a0", "resource_type": "article", "pub_year": "1988", "author_list": "Bronner-Fraser, Marianne and Fraser, Scott E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/3nrpk-5qa15", "eprint_id": 66158, "eprint_status": "archive", "datestamp": "2023-08-19 20:35:15", "lastmod": "2023-10-18 17:12:51", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas E." }, "orcid": "0000-0001-6577-6676" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "A Spatial and Temporal Analysis of Dorsal Root and Sympathetic Ganglion Formation in the Avian Embryo", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1988 Academic Press, Inc. \n\nAccepted January 20, 1988. \n\nWe thank Drs. Scott Fraser and Roberto Perris for critical reading of the manuscript. This work was supported by USPHS Grant HD15527, NSF Grant DCB8502604, and Basic Research Grant 1-896 from the March of Dimes. M.B.-F. is a Sloan Foundation Fellow.", "abstract": "The present study explores the formation of the dorsal root and sympathetic ganglia in the trunk of the avian embryo. Particular emphasis was given to the timing of gangliogenesis and the relative positions of the neural crest-derived ganglia with respect to the somites. Neural crest cells and their derivatives were recognized by the HNK-1 antibody. The time at which neural crest cell coalesced to form ganglia was assessed by the state of cellular aggregation. The state of ganglionic differentiation was assessed by the expression of neurofilament proteins and the neural cell adhesion molecule (N-CAM). At the level of the 15th somite, neural crest cells were observed in the rostral half of the somite at stage 15, during active neural crest migration, and occupied the rostral two-thirds of the somite at progressive stages. HNK-1 positive cells appeared to be organized in three to four streams of cells oriented mediolaterally and dorsoventrally. The dorsal root ganglia and sympathetic ganglia were first detectable at stages 20 and 21, respectively. Both ganglionic rudiments were aligned with the rostral portion of the somite. The dorsal root ganglia occupied the rostral two-thirds of each somite, whereas cells in the sympathetic ganglia occupied a region corresponding to approximately one-third of each somite. At the time of condensation of the dorsal root ganglia, abundant neurofilament staining was observed within the ganglia. However, no N-CAM immunoreactivity was detected until three stages later at stage 23. In contrast, the sympathetic ganglia demonstrated both neurofilament and N-CAM immunoreactivity at the time of condensation. The observation that both dorsal root and sympathetic ganglia form in register with the rostral portion of somite suggests that cues localized at these axial levels, perhaps within the rostral somite, may influence the position where neural crest cells condense to form ganglia. In sensory ganglia, N-CAM expression does not correlate with the onset of gangliogenesis, suggesting that molecules other than N-CAM may play an important role in the aggregation of some neuronal populations.", "date": "1988-05", "date_type": "published", "publication": "Developmental Biology", "volume": "127", "number": "1", "publisher": "Elsevier", "pagerange": "99-112", "id_number": "CaltechAUTHORS:20160414-093718066", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-093718066", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD15527" }, { "agency": "NSF", "grant_number": "DCB8502604" }, { "agency": "March of Dimes", "grant_number": "1-896" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(88)90192-3", "resource_type": "article", "pub_year": "1988", "author_list": "Lallier, Thomas E. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/32w4s-cb676", "eprint_id": 66185, "eprint_status": "archive", "datestamp": "2023-08-19 20:35:29", "lastmod": "2023-10-18 17:14:33", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krotoski-Dantua-M", "name": { "family": "Krotoski", "given": "Danuta M." } }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Mapping of Neural Crest Pathways in Xenopus laevis Using Inter- and Intra-specific Cell Markers", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1988 Academic Press, Inc. \n\nAccepted January 21, 1988. \n\nWe thank Drs. Gabrielle Leblanc, Roberto Perris, and James Coulombe for helpful comments on the manuscript, Dr. Nancy O'Rourke for providing lysinated dextran embryos, and Dr. George V. Lauder, Jr., for help with statistical analyses. This work was supported by USPHS Grant HD15527 and NSF Grants BNS 8607760 and BNS 8608356. M.B.-F. is a Sloan Foundation Fellow.", "abstract": "This study examines the pathways of migration followed by neural crest cells in Xenopus embryos using two recently described cell marking techniques. The first is an interspecific chimera created by grafting Xenopus borealis cells into Xenopus laevis hosts. The cells of these closely related species can be distinguished by their nuclear dimorphism. The second type of marker is created by microinjection of lysinated dextrans into fertilized eggs which can then be used for intraspecific grafting. These recently developed fluorescent dyes are fixable and identifiable in both living and fixed embryos. After grafting labeled donor neural tubes into unlabeled host embryos, the distribution of neural crest cells at various stages after grafting was used to define the pathways of neural crest migration. To control for possible grafting artifacts, fluorescent lysinated dextran was injected into a single blastomere which gives rise to a large number of neural crest cells, thereby labeling the neural crest without grafting. By all three techniques, Xenopus neural crest cells were observed along two predominant pathways in the trunk. The majority of neural crest cells were observed along a \"ventral\" route, between the neural tube and somite, the notochord and somite, and along the dorsal mesentery. A second group of neural crest cells was observed \"dorsally\" where they populated the dorsal fin. A third minor \"lateral\" pathway was observed primarily in borealis/laevis chimerae and in blastomere-injected embryos; some neural crest cells were observed underneath the ectoderm lateral to the neural tube. Along the rostrocaudal axis, neural crest cells were not continuously distributed but were primarily located across from the caudal two-thirds of the somite. Fewer than 3% of the neural crest cells were observed across from the rostral third of each somite. When grafted to ventral locations, neural crest cells were not able to migrate dorsally but migrated laterally along the dorsal mesentery. Labeled neural crest cells gave rise to cells of the spinal, sympathetic, and enteric ganglia as well as to adrenal chromaffin cells, Schwann cells, pigment cells, mesenchymal cells of the dorsal fin, and some cells in the integuments and in the region of the pronephros. These results show that the neural crest migratory pathways in Xenopus differ from those in the avian embryo. In avians NC cells migrate as a closely associated sheet of cells while in Xenopus they migrate as individual cells. Both species exhibit a metamerism in the neural crest cell distribution pattern along the rostrocaudal axis. However, in chick embryos NC cells migrate through the rostral sclerotome of each somite and are never observed in the perinotochordal area.", "date": "1988-05", "date_type": "published", "publication": "Developmental Biology", "volume": "127", "number": "1", "publisher": "Elsevier", "pagerange": "119-132", "id_number": "CaltechAUTHORS:20160414-144014671", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-144014671", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "NSF", "grant_number": "BNS 8607760" }, { "agency": "NSF", "grant_number": "BNS 8608356" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(88)90194-7", "resource_type": "article", "pub_year": "1988", "author_list": "Krotoski, Danuta M.; Fraser, Scott E.; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/5j8tq-j4465", "eprint_id": 67095, "eprint_status": "archive", "datestamp": "2023-08-19 20:31:52", "lastmod": "2023-10-18 20:05:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Lallier-Thomas-E", "name": { "family": "Lallier", "given": "Thomas" }, "orcid": "0000-0001-6577-6676" } ] }, "title": "A monoclonal antibody against a laminin-heparan sulfate proteoglycan complex perturbs cranial neural crest migration in vivo", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1988 Rockefeller University Press. The Authors acknowledge that RUP will make the Article freely available to the public on RUP's website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central's requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nReceived for publication 13 July 1987, and in revised form 17 December 1987. Published April 1, 1988. \n\nWe thank Dr. Scott Fraser for helpful comments on the manuscript, and Georgia Guillory and Carmen Domingo for excellent technical assistance. This work was supported by U.S. Public Health Service Grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes. M. Bronner-Fraser is a Sloan Foundation Fellow.\n\nPublished - J_Cell_Biol-1988-Bronner-Fraser-1321-9.pdf
", "abstract": "INO (inhibitor of neurite outgrowth) is a monoclonal antibody that blocks axon outgrowth, presumably by functionally blocking a laminin-heparan sulfate proteoglycan complex (Chiu, A. Y., W. D. Matthew, and P. H. Patterson. 1986. J. Cell Biol. 103: 1382-1398). Here the effect of this antibody on avian neural crest cells was examined by microinjecting INO onto the pathways of cranial neural crest migration. After injection lateral to the mesencephalic neural tube, the antibody had a primarily unilateral distribution. INO binding was observed in the basal laminae surrounding the neural tube, ectoderm, and endoderm, as well as within the cranial mesenchyme on the injected side of the embryo. This staining pattern was indistinguishable from those observed with antibodies against laminin or heparan sulfate proteoglycan. The injected antibody remained detectable for 18 h after injection, with the intensity of immuno-reactivity decreasing with time. Embryos ranging from the neural fold stage to the 9-somite stage were injected with INO and subsequently allowed to survive for up to 1 d after injection. These embryos demonstrated severe abnormalities in cranial neural crest migration. The predominant defects were ectopic neural crest cells external to the neural tube, neural crest cells within the lumen of the neural tube, and neural tube deformities. In contrast, embryos injected with antibodies against laminin or heparan sulfate proteoglycan were unaffected. When embryos with ten or more somites were injected with INO, no effects were noted, suggesting that embryos are sensitive for only a limited time during their development. Immunoprecipitation of the INO antigen from 2-d chicken embryos revealed a 200-kD band characteristic of laminin and two broad smears between 180 and 85 kD, which were resolved into several bands at lower molecular mass after heparinase digestion. These results indicate that INO precipitates both laminin and proteoglycans bearing heparan sulfate residues. Thus, microinjection of INO causes functional blockage of a laminin-heparan sulfate proteoglycan complex, resulting in abnormal cranial neural crest migration. This is the first evidence that a laminin-heparan sulfate proteoglycan complex is involved in aspects of neural crest migration in vivo.", "date": "1988-04", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "106", "number": "4", "publisher": "Rockefeller University Press", "pagerange": "1321-1329", "id_number": "CaltechAUTHORS:20160516-080748233", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160516-080748233", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "HD-15527" }, { "agency": "March of Dimes", "grant_number": "1-896" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1083/jcb.106.4.1321", "pmcid": "PMC2114992", "primary_object": { "basename": "J_Cell_Biol-1988-Bronner-Fraser-1321-9.pdf", "url": "https://authors.library.caltech.edu/records/5j8tq-j4465/files/J_Cell_Biol-1988-Bronner-Fraser-1321-9.pdf" }, "resource_type": "article", "pub_year": "1988", "author_list": "Bronner-Fraser, Marianne and Lallier, Thomas" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/tyd43-g1k65", "eprint_id": 63278, "eprint_status": "archive", "datestamp": "2023-08-19 20:07:05", "lastmod": "2023-10-25 23:41:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Fraser-S-E", "name": { "family": "Fraser", "given": "Scott E." }, "orcid": "0000-0002-5377-0223" } ] }, "title": "Application of new technologies to studies of neural crest migration and differentiation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1988 Wiley-Liss, Inc.", "abstract": "This review describes the application of new techniques for examining some longstanding questions in the neural crest system concerning pathways of migration, cell lineage decisions, and importance of the extracellular matrix. The first issue examined involves the migratory pathways followed by neural crest cells. In birds, it has been possible to map crest migratory routes accurately using antibodies that selectively recognize neural crest cells. These antibodies permit the identification of migrating cells in the absence of the surgical trauma incurred by neural tube transplantations. In amphibian embryos, which are more readily accessible to embryological manipulation than are birds, neural fold grafts using two new cell markers have made it possible to map the early stages of neural crest migration using both interspecific and intraspecific chimerae. In both birds and amphibians, a metameric pattern of neural crest migration was observed; this presumably results from interactions with the adjacent somites. \n\nA second novel experimental paradigm tests the role of cell surface\u2013extracellular matrix interactions in neural crest migration. Using antibodies to perturb interactions between the cell surface and the extracellular matrix selectively, it has been possible to identify some molecular interactions that are important for aspects of neural crest migration in situ. \n\nNew techniques have also made it possible to examine the cell lineage decisions of neural crest cells. Using a retrograde labeling technique and a method of microinjecting cells into embryos, we have found that neural crest-derived cholinergic neurons remain plastic with respect to their neurotransmitter expression even after overt differentiation. New cell-marking techniques have made it possible to study neural crest cell lineage by means of clonal analysis. Individual neural crest precursors can be injected with a cell lineage tracer that is passed to all of its progeny. Thus, we can examine the developmental potential of individual neural crest cells. The various experimental paradigms presented in this review illustrate the utility of recent technological advances to study embryological questions that have long concerned investigators interested in the neural crest and in other developmental systems.", "date": "1988", "date_type": "published", "publication": "American Journal of Medical Genetics", "volume": "31", "number": "S1", "publisher": "Wiley-Liss", "pagerange": "23-39", "id_number": "CaltechAUTHORS:20151230-221442726", "issn": "0148-7299", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151230-221442726", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "doi": "10.1002/ajmg.1320310509", "resource_type": "article", "pub_year": "1988", "author_list": "Bronner-Fraser, Marianne and Fraser, Scott E." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w9pzr-5q893", "eprint_id": 66152, "eprint_status": "archive", "datestamp": "2023-08-19 19:50:57", "lastmod": "2023-10-18 17:12:24", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Perturbation of Cranial Neural Crest Migration by the HNK-1 Antibody", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1987 Academic Press, Inc. \n\nReceived September 12, 1986; accepted in revised form June 1, 1987. \n\nI thank Drs. Scott Fraser, Gabrielle Leblanc, James Coulombe, and Roberto Perris for helpful comments on the manuscript, Georgia Guillory for excellent technical assistance, and Thomas Lallier for preparation of small IgM fragments. This work was supported by USPHS Grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes. M.B.-F. is a Sloan Foundation Fellow.", "abstract": "The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tube explants in tissue culture, neural crest cells detached from laminin substrates but were unaffected on fibronectin substrates. In order to examine the effects of the HNK-1 antibody in vivo, antibody was injected lateral to the mesencephalic neural tube at the onset of cranial neural crest migration. The injected antibody persisted for approximately 16 hr on the injected side of the embryo and appeared to be most prevalent on the surface of neural crest cells. Embryos fixed within the first 24 hr after injection of HNK-1 antibodies (either whole IgMs or small IgM fragments) showed one or more of the following abnormalities: (1) ectopic neural crest cells external to the neural tube, (2) an accumulation of neural crest cells in the lumen of the neural tube, (3) some neural tube anomalies, or (4) a reduction in the neural crest cell volume on the injected side. The ectopic cells and neural tube anomalies persisted in embryos fixed 2 days postinjection. Only embryos having 10 or less somites at the time of injection were affected, suggesting a limited period of sensitivity to the HNK-1 antibody. Control embryos injected with a nonspecific antibody or with a nonblocking antibody against the neural cell adhesion molecule (N-CAM) were unaffected. Previous experiments from this laboratory have demonstrated than an antibody against integrin, a fibronectin and laminin receptor caused defects qualitatively similar to those resulting from HNK-1 antibody injection (M. Bronner-Fraser, J. Cell Biol., 101, 610, 1985). Coinjection of the HNK-1 and integrin antibodies resulted in a greater percentage of affected embryos than with either antibody alone. The additive nature of the effects of the two antibodies suggests that they act at different sites. These results demonstrate that the HNK-1 antibody causes abnormalities in cranial neural crest migration, perhaps by perturbing interactions between neural crest cells and laminin substrates.", "date": "1987-10", "date_type": "published", "publication": "Developmental Biology", "volume": "123", "number": "2", "publisher": "Elsevier", "pagerange": "321-331", "id_number": "CaltechAUTHORS:20160414-085615839", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-085615839", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes", "grant_number": "1-896" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(87)90390-3", "resource_type": "article", "pub_year": "1987", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1bm59-1n678", "eprint_id": 66160, "eprint_status": "archive", "datestamp": "2023-08-19 19:48:09", "lastmod": "2023-10-18 17:13:02", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Smith-Thomas-L", "name": { "family": "Smith-Thomas", "given": "Linda" } }, { "id": "Lott-I", "name": { "family": "Lott", "given": "Ira" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Effects of lsotretinoin on the Behavior of Neural Crest Cells in Vitro", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1987 Academic Press, Inc. \n\nReceived November 3, 1986; accepted in revised farm April 20, 1987. \n\nWe are grateful to Dr. Peter Sorter and Hoffman La-Roche for providing us with isotretinoin. We thank Drs. Gabrielle LeBlanc, Danuta Krotoski, James Coulombe, Georgia Guillory, and Tom Lallier for their helpful comments on the manuscript. This work was supported by Monsanto Company and Basic Research Grant 1-896 from the March of Dimes Birth Defects Foundation. M. Bronner-Fraser is a Sloan Fellow.", "abstract": "Isotretinoin (13-cis-retinoic acid), an anti-acne medication, has been found to cause severe birth defects which affect the craniofacial elements, ear, heart, thymus, and central nervous system. Many of these structures receive contributions from the cranial neural crest. Here, we examine the possibility that these teratogenic effects are due to disturbances in neural crest development. Cranial and trunk neural crest explant cultures were exposed to different concentrations of isotretinoin and the cell morphology was monitored at daily intervals. Treated neural crest cells often became rounded or spindle shaped, separated from their neighbors, and frequently detached from the substrate or clumped together. In contrast, neural tube cells and cardiac fibroblasts were relatively unaffected by the drug. These results suggest that isotretinoin selectively affects neural crest cells by decreasing their cell-substratum adhesion.", "date": "1987-09", "date_type": "published", "publication": "Developmental Biology", "volume": "123", "number": "1", "publisher": "Elsevier", "pagerange": "276-281", "id_number": "CaltechAUTHORS:20160414-093724625", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-093724625", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "Monsanto Company" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "1-896" }, { "agency": "Alfred P. Sloan Foundation" } ] }, "doi": "10.1016/0012-1606(87)90449-0", "resource_type": "article", "pub_year": "1987", "author_list": "Smith-Thomas, Linda; Lott, Ira; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pthdr-pkj83", "eprint_id": 67127, "eprint_status": "archive", "datestamp": "2023-08-19 19:01:21", "lastmod": "2023-10-18 20:34:22", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coulombe-J-N", "name": { "family": "Coulombe", "given": "James Niles" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Cholinergic neurones acquire adrenergic neurotransmitters when transplanted into an embryo", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1986 Nature Publishing Group. \n\nReceived 27 June; accepted 18 September 1986. \n\nWe thank Dr Scott Fraser for his helpful comments on the manuscript and Georgia Guillory and Jeffrey D. Pratt for technical assistance. This work was supported by USPHS Grant HD-15527-01 and by Basic Research Grant 1-896 from the March of Dimes.", "abstract": "During development, cells become progressively restricted, until they reach their final phenotype. Differentiation was originally thought to be irreversible, but phenotypic plasticity has been observed in a variety of cell types, for example sympathetic neurones, the limb blastema and some glial cell types. A detailed description of the individual steps that lead to expression or reversal of phenotype is essential to understand the molecular events underlying cell differentiation. We examined whether ciliary neurones acquire adrenergic properties when exposed to a permissive embryonic environment. Cholinergic neurones were selectively labelled with a retrogradely transported marker and injected into chick embryos during active neural crest migration. Four to five days after injection, some of the labelled neurones were found in 'adrenergic sites' and had developed catecholamine histofluorescence. The cells had thus accumulated adrenergic neurotransmitters even after differentiation into Cholinergic neurones. This result shows that neurotransmitter plasticity occurs in Cholinergic neurones and suggests that the neurotransmitter phenotype can be modified by the embryonic environment.", "date": "1986-12-11", "date_type": "published", "publication": "Nature", "volume": "324", "number": "6097", "publisher": "Nature Publishing Group", "pagerange": "569-572", "id_number": "CaltechAUTHORS:20160516-110121666", "issn": "0028-0836", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160516-110121666", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes", "grant_number": "1-896" } ] }, "doi": "10.1038/324569a0", "resource_type": "article", "pub_year": "1986", "author_list": "Coulombe, James Niles and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/fa34w-m3j98", "eprint_id": 32124, "eprint_status": "archive", "datestamp": "2023-08-19 18:54:58", "lastmod": "2023-10-17 22:59:07", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Guillory-G", "name": { "family": "Guillory", "given": "Georgia" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "An in vitro assay for neural crest cell migration through the somites", "ispublished": "pub", "full_text_status": "public", "keywords": "neural crest, migration, somites, pigment cells, in vitro, quail, Coturnix coturnix\njaponica.", "note": "\u00a9 1986 The Company of Biologists Limited. \n\nAccepted April 22, 1986. \n\nWe would like to thank Dr Scott Fraser for his helpful comments on the manuscript. This work was supported by USPHS Grant HD-15527-01 and Basic Research Grant 1-896 from the March of Dimes.\n\nPublished - GUIdev86.pdf
", "abstract": "Neural crest cells in the trunk of the avian embryo come into contact with the somites and neural tube during the course of their migration. However, the relationship between the somites and the early migratory routes followed by these cells is not yet completely understood. Here, we use a tissue culture assay to examine if avian neural crest cells migrate through the somites. Cultures of quail somites were prepared from four adjacent regions along the neural axis in the trunk. Each region had four pairs of consecutive somites with region I being most anterior and region IV containing the last four segments. Within each region, the somites were separated from other tissues by enzymatic digestion and plated onto collagen-coated dishes. Immuno-cytochemical techniques were used to confirm that no neural crest cells, recognized by the HNK-1 antibody, were present on the surface of the somites at the time of explantation. After several days in culture, the explanted somites were screened to identify pigment cells. Because neural crest cells give rise to all of the melanocytes in the trunk, the presence of pigment cells indicated that neural crest precursors were contained within the initial explant. After 5\u201311 days in vitro, the percentage of somite cultures containing pigment cells in regions I through IV, respectively, was 36%, 51%, 31% and 1%. These results suggest that neural crest cells migrate through the somitic mesenchyme and first enter the somites between 5 to 9 segments rostral to the most recently formed somite.", "date": "1986-11", "date_type": "published", "publication": "Journal of Embryology and Experimental Morphology", "volume": "98", "number": "1", "publisher": "Company of Biologists", "pagerange": "85-97", "id_number": "CaltechAUTHORS:20120627-082546827", "issn": "0022-0752", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20120627-082546827", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes", "grant_number": "1-896" } ] }, "primary_object": { "basename": "GUIdev86.pdf", "url": "https://authors.library.caltech.edu/records/fa34w-m3j98/files/GUIdev86.pdf" }, "resource_type": "article", "pub_year": "1986", "author_list": "Guillory, Georgia and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/pk4np-n9t89", "eprint_id": 66155, "eprint_status": "archive", "datestamp": "2023-08-19 18:52:02", "lastmod": "2023-10-18 17:12:40", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "An Antibody to a Receptor for Fibronectin and Laminin Perturbs Cranial Neural Crest Development in Vivo", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1986 Academic Press, Inc. \n\nReceived February 19, 1986; accepted in revised form April 28, 1986. \n\nI thank Dr. Scott Fraser for helpful comments on the manuscript and Leena Carriere, Carmen Domingo, and Georgia Guillory for their technical assistance. This work was supported by USPHS HD-15527 and by a Basic Research Grant from the March of Dimes Birth Defects Foundation.", "abstract": "Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alternations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody, the dose dependency of the effect, and the long-term influences of antibody injection. The CSAT antibody, which recognizes a cell surface receptor for fibronectin and laminin, was injected lateral to the mesencephalic neural tube at the onset of cranial neural crest migration. Injected antibody molecules did not cross the midline, but appeared to diffuse throughout the injected half of the mesencephalon, where they remained detectable by immunocytochemistry for about 22 hr. Embryos were examined either during neural crest migration (up to 24 hr after injection) or after formation of neural crest-derived structures (36\u201348 hr after injection). In those embryo fixed within the first 24 hr, the major defects were a reduction in the neural crest cell number on the injected side, a buildup of neural crest cells within the lumen of the neural tube, and ectopically localized neural crest cells. In embryos allowed to survive for 36 to 48 hr after injection, the neural crest derivatives appeared normal on both the injected and control side, suggesting that the embryos compensated for the reduction in neural crest cell number on the injected side. However, the embryos often had severely deformed neural tubes and ectopic aggregates of neural crest cells. In contrast, several control antibodies had no effect. These findings suggest that the CSAT receptor complex is important in the normal development of the neural crest and neural tube.", "date": "1986-10", "date_type": "published", "publication": "Developmental Biology", "volume": "117", "number": "2", "publisher": "Elsevier", "pagerange": "528-536", "id_number": "CaltechAUTHORS:20160414-085616756", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-085616756", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "doi": "10.1016/0012-1606(86)90320-9", "resource_type": "article", "pub_year": "1986", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/y388e-dbt54", "eprint_id": 67088, "eprint_status": "archive", "datestamp": "2023-08-19 18:49:39", "lastmod": "2023-10-18 19:57:29", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krotoski-D-M", "name": { "family": "Krotoski", "given": "Danuta M." } }, { "id": "Domingo-C", "name": { "family": "Domingo", "given": "Carmen" } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Distribution of a putative cell surface receptor for fibronectin and laminin in the avian embryo", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1986 Rockefeller University Press. The Authors acknowledge that RUP will make the Article freely available to the public on RUP's website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central's requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nReceived for publication 14 February 1986, and in revised form 30 May 1986. \n\nWe thank Drs. Scott Fraser, James Coulombe, Marcia Yaross, and Roberto Perris for their helpful comments on the manuscript. This work was supported by United States Public Health Services grant HD-15527-01 and a Basic Research Grant from the March of Dimes Birth Defects Foundation.\n\nPublished - J_Cell_Biol-1986-Krotoski-1061-71.pdf
", "abstract": "The cell substratum attachment (CSAT) antibody recognizes a 140-kD cell surface receptor complex involved in adhesion to fibronectin (FN) and laminin (LM) (Horwitz, A., K. Duggan, R. Greggs, C. Decker, and C. Buck, 1985, J. Cell Biol., 101:2134-2144). Here, we describe the distribution of the CSAT antigen along with FN and LM in the early avian embryo. At the light microscopic level, the staining patterns for the CSAT receptor and the extracellular matrix molecules to which it binds were largely codistributed. The CSAT antigen was observed on numerous tissues during gastrulation, neurulation, and neural crest migration: for example, the surface of neural crest cells and the basal surface of epithelial tissues such as the ectoderm, neural tube, notochord, and dermomyotome. FN and LM immunoreactivity was observed in the basement membranes surrounding many of these epithelial tissues, as well as around the otic and optic vesicles. In addition, the pathways followed by cranial neural crest cells were lined with FN and LM. In the trunk region, FN and LM were observed surrounding a subpopulation of neural crest cells. However, neither molecule exhibited the selective distribution pattern necessary for a guiding role in trunk neural crest migration. The levels of CSAT, FN, and LM are dynamic in the embryo, perhaps reflecting that the balance of surface-substratum adhesions contributes to initiation, migration, and localization of some neural crest cell populations.", "date": "1986-09-01", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "103", "number": "3", "publisher": "Rockefeller University Press", "pagerange": "1061-1071", "id_number": "CaltechAUTHORS:20160513-135631179", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160513-135631179", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "doi": "10.1083/jcb.103.3.1061", "pmcid": "PMC2114279", "primary_object": { "basename": "J_Cell_Biol-1986-Krotoski-1061-71.pdf", "url": "https://authors.library.caltech.edu/records/y388e-dbt54/files/J_Cell_Biol-1986-Krotoski-1061-71.pdf" }, "resource_type": "article", "pub_year": "1986", "author_list": "Krotoski, Danuta M.; Domingo, Carmen; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/0tg1w-7zh94", "eprint_id": 66153, "eprint_status": "archive", "datestamp": "2023-08-19 18:48:27", "lastmod": "2023-10-18 17:12:31", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Howard-M-J", "name": { "family": "Howard", "given": "Marthe J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Neural Tube-Derived Factors Influence Differentiation of Neural Crest Cells in Vitro: Effects on Activity of Neurotransmitter Biosynthetic Enzymes", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1986 Academic Press, Inc. \n\nReceived July 16, 1985; accepted in revised form March 20, 1986. \n\nWe thank Dr. Darwin Berg, Dr. James Coulombe, Dr. Scott Fraser, and Dr. Joseph Margiotta for helpful comments on the manuscript, and Georgia Guillory for technical assistance. This work was partially supported by USPHS Grant 15527-01 and a Basic Research Grant for the March of Dimes Birth Defects Foundation.", "abstract": "Previously, we have demonstrated that a factor present in chick embryo extract or medium conditioned by neural tube cells supports adrenergic differentiation of some neural crest cells in vitro. These studies have been extended here to examine the effects of this factor(s) on the development of enzymes involved in neurotransmitter biosynthesis. The time course of expression of choline acetyltransferase (ChAT), a marker for cholinergic cells, and dopamine-\u03b2-hydroxylase (DBH), a marker for adrenergic cells, was examined in neural crest cell cultures grown under three conditions: (1) in medium containing 10% embryo extract, (2) in medium containing 2% embryo extract, and (3) in medium containing 2% embryo extract that was conditioned by neural tube cells (NTCM). Significant levels of DBH activity were measured in neural crest cell cultures grown in 10% embryo extract containing medium or in NTCM, while only low levels were present in cultures grown in medium containing 2% embryo extract. In contrast, ChAT activity was inhibited by NTCM in comparison to levels in both 10 and 2% embryo extract containing medium. As a preliminary characterization of the factor(s) present in chick embryo extract, we have fractionated embryo extract and find that a pool of 10 kDa or less can support adrenergic differentiation of some neural crest cells. These results suggest that low molecular weight factors present in embryo extract and NTCM support adrenergic expression of neural crest cells, whereas NTCM suppresses cholinergic expression.", "date": "1986-09", "date_type": "published", "publication": "Developmental Biology", "volume": "117", "number": "1", "publisher": "Elsevier", "pagerange": "45-54", "id_number": "CaltechAUTHORS:20160414-085616155", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-085616155", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "15527-01" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "doi": "10.1016/0012-1606(86)90346-5", "resource_type": "article", "pub_year": "1986", "author_list": "Howard, Marthe J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/70td0-n1d12", "eprint_id": 66156, "eprint_status": "archive", "datestamp": "2023-08-19 18:36:01", "lastmod": "2023-10-18 17:12:44", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Analysis of the Early Stages of Trunk Neural Crest Migration in Avian Embryos Using Monoclonal Antibody HNK-1", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1986 Academic Press. Inc. \n\nReceived May 15, 1985; accepted in revised form December 16, 1985. \n\nI thank Dr. Scott Fraser and Dr. James Coulombe for their helpful comments on the manuscript, and Georgia Guillory and Jeffrey Pratt for their excellent technical assistance. This work was supported by USPHS Grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes.", "abstract": "The monoclonal antibody HNK-1 was used to identify neural crest cells in serial sections of avian embryos to provide a detailed description of the distribution of trunk neural crest cells. The results indicate the presence of three migratory routes in the trunk: (1) a ventral pathway through the anterior sclerotome; (2) a ventral pathway between the neural tube and the posterior sclerotome; and (3) a dorsolateral pathway between the somites and ectoderm. Neural crest cells were first seen entering the anterior half of the sclerotome at about the time the somite begins to dissociate to form the dermomyotome and sclerotome, approximately 5\u201310 somites rostral to the most recently formed somite. In contrast, neural crest cells were never observed in the posterior sclerotome or in the perinotochordal space. The distribution of neural crest cells was compared with that of injected latex beads which were previously found to translocate along the ventral trunk neural crest pathway (Bronner-Fraser, Dev. Biol. 91, 130, 1982). During the early stages of neural crest migration, injected latex beads were found to extensively colocalize with cells stained by the HNK-1 antibody. Injected latex beads (78%) were found immediately adjacent to HNK-1 positive cells and another 11% were within one cell diameter. The results suggest that latex beads injected into the trunk somites are deposited onto a normal pathway of neural crest migration.", "date": "1986-05", "date_type": "published", "publication": "Developmental Biology", "volume": "115", "number": "1", "publisher": "Elsevier", "pagerange": "44-55", "id_number": "CaltechAUTHORS:20160414-085617023", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-085617023", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527" }, { "agency": "March of Dimes", "grant_number": "1-896" } ] }, "doi": "10.1016/0012-1606(86)90226-5", "resource_type": "article", "pub_year": "1986", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/t4xn2-nt289", "eprint_id": 63289, "eprint_status": "archive", "datestamp": "2023-08-19 18:15:47", "lastmod": "2024-01-13 16:30:33", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Guidance of Neural Crest Migration: Latex Beads as Probes of Surface\u2014Substratum Interactions", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 1986 Plenum Press. \n\nI would like to thank Virginia Bayer for illustrations. Parts of the research in this article were supported by the U.S. Public Health Service Grant HD-15527-01 and by Basil O'Connor Starter Research Grant 5-312 from the March of Dimes Birth Defects Foundation.", "abstract": "The vertebrate neural crest is first recognizable at the onset of neurulation as the neural folds appose to form the neural tube. Neural crest cells emigrate from the dorsal neural tube shortly after tube closure. From this site of origin, they migrate extensively and differentiate into a wide variety of derivatives. Neural crest-derived cell types include neurons and supportive cells of the peripheral nervous system, melanocytes, several neurosecretory cell types, bone and car tilage of the face, and adrenal chromaffin cells. Because of the migratory ability of neural crest cells and the diversity of crest derivatives, this transient embryonic structure provides an important model system for studying interactions involved in cell movement and differentiation.", "date": "1986", "date_type": "published", "publisher": "Plenum Press", "place_of_pub": "New York, NY", "pagerange": "301-337", "id_number": "CaltechAUTHORS:20160101-143100520", "isbn": "978-1-4684-5052-1", "book_title": "The Cell Surface in Development and Cancer", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-143100520", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "5-312" } ] }, "contributors": { "items": [ { "id": "Steinberg-M-S", "name": { "family": "Steinberg", "given": "Malcolm S." } } ] }, "doi": "10.1007/978-1-4684-5050-7_15", "resource_type": "book_section", "pub_year": "1986", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/1ycc3-exm21", "eprint_id": 63288, "eprint_status": "archive", "datestamp": "2023-08-19 18:15:43", "lastmod": "2023-10-25 23:42:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Krotoski-D-M", "name": { "family": "Krotoski", "given": "D. M." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "M." }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Mapping of neural crest pathways in Xenopus laevis", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1986 Wiley-Liss.", "date": "1986", "date_type": "published", "publication": "Progress in clinical and biological research", "volume": "217B", "publisher": "Wiley-Liss", "pagerange": "229-33", "id_number": "CaltechAUTHORS:20160101-142927520", "issn": "0361-7742", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-142927520", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "article", "pub_year": "1986", "author_list": "Krotoski, D. M. and Bronner-Fraser, M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/vdnfg-wwa55", "eprint_id": 67157, "eprint_status": "archive", "datestamp": "2023-08-19 18:07:02", "lastmod": "2023-10-18 20:36:28", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Howard-M-J", "name": { "family": "Howard", "given": "Marthe J." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "The Influence of Neural Tube-derived Factors on Differentiation of Neural Crest Cells In Vitro.I. Histochemical Study on the Appearance of Adrenergic Cells", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1985 Society for Neuroscience. For the first six months after publication SfN's license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). \n\nReceived February 27, 1985; Revised April 22, 1985; Accepted April 22, 1985. \n\nWe thank Drs. Scott Fraser and James Coulombe for helpful comments on the manuscript, and Georgia Guillory for her excellent technical assistance. This work was partially supported by United States Public Health Service Grant 15527-01 and a Basic Research grant for the March of Dimes Birth Defects Foundation.\n\nPublished - 3302.full.pdf
", "abstract": "The neural crest gives rise to numerous derivatives including adrenergic and cholinergic neurons, supportive cells of the nervous system, and melanocytes. In tissue culture, neural crest cells explanted from both cranial and trunk regions were found to differentiate into adrenergic neuroblasts or into pigmented cells when grown in medium containing 10% chick embryo extract. When the embryo extract concentration was lowered to 2%, no catecholamine-containing cells (as assayed by formaldehyde-induced fluorescence) were detected, although pigment cells were observed. These results suggest the presence of a factor(s) in embryo extract that promotes or supports adrenergic differentiation. To examine the possible tissue sources of this factor(s), neural tube, notochord, or somite cells were used to condition medium containing 2% embryo extract. When neural crest cells were grown in medium conditioned by neural tube cells, adrenergic neuroblasts were observed in all cultures. However, somite- and notochord conditioned medium did not support adrenergic differentiation. In addition, medium supplemented with extracts derived from central nervous system components did support adrenergic expression, whereas medium supplemented with embryo extract from which the neural tissue was removed did not. Direct contact with neural tube cell ghost membranes was unable to substitute for high embryo extract concentrations or for neural tube-conditioned medium. These results suggest that the neural tube makes a diffusible factor(s) that will support adrenergic differentiation of neural crest cells.", "date": "1985-12", "date_type": "published", "publication": "Journal of Neuroscience", "volume": "5", "number": "12", "publisher": "Society for Neuroscience", "pagerange": "3302-3309", "id_number": "CaltechAUTHORS:20160517-104614514", "issn": "0270-6474", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160517-104614514", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "15527-01" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "doi": "10.1523/jneurosci.05-12-03302.1985", "pmcid": "PMC6565217", "primary_object": { "basename": "3302.full.pdf", "url": "https://authors.library.caltech.edu/records/vdnfg-wwa55/files/3302.full.pdf" }, "resource_type": "article", "pub_year": "1985", "author_list": "Howard, Marthe J. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jqv96-s0y31", "eprint_id": 67096, "eprint_status": "archive", "datestamp": "2023-08-19 17:55:57", "lastmod": "2023-10-18 20:05:18", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1985 Rockefeller University Press. The Authors acknowledge that RUP will make the Article freely available to the public on RUP's website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central's requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nReceived for publication 22 March 1985, and in revised form 27 April 1985. \n\nI thank Drs. Scott Fraser and James Coulombe for their helpful comments on the manuscript, and Georgia Guillory and Elizabeth Montgomery for their excellent technical assistance. This work was supported by U.S. Public Health Service grant HD-15527-01 and by a Basic Research Grant from the March of Dimes Birth Defects Foundation.\n\nPublished - J_Cell_Biol-1985-Bronner-Fraser-610-7.pdf
", "abstract": "The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest cells in vitro caused a rapid change in morphology of cells plated on either fibronectin or laminin substrates. The cells became round and phase bright, often detaching from the dish or forming aggregates of rounded cells. Other tissues such as somites, notochords, and neural tubes were unaffected by the antibody in vitro even though the JG22 antigen is detectable in embryonic tissue sections on the surface of the myotome, neural tube, and notochord. The effects of the JG22 on neural crest migration in vivo were examined by a new perturbation approach in which both the antibody and the hybridoma cells were microinjected onto neural crest pathways. Hybridoma cells were labeled with a fluorescent cell marker that is nondeleterious and that is preserved after fixation and tissue sectioning. The JG22 antibody and hybridoma cells caused a marked reduction in cranial neural crest migration, a build-up of neural crest cells within the lumen of the neural tube, and some migration along aberrant pathways. Neural crest migration in the trunk was affected to a much lesser extent. In both cranial and trunk regions, a cell free zone of one or more cell diameters was generally observed between neural crest cells and the JG22 hybridoma cells. Two other monoclonal antibodies, 1-B and 1-N, were used as controls. Both 1-B and 1-N bind to bands of the 140-kD complex precipitated by JG22. Neither control antibody affected neural crest adhesion in vitro or neural crest migration in situ. This suggests that the observed alterations in neural crest migration are due to a functional block of the 140-kD complex.", "date": "1985-08", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "101", "number": "2", "publisher": "Rockefeller University Press", "pagerange": "610-617", "id_number": "CaltechAUTHORS:20160516-081820402", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160516-081820402", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation" } ] }, "doi": "10.1083/jcb.101.2.610", "pmcid": "PMC2113653", "primary_object": { "basename": "J_Cell_Biol-1985-Bronner-Fraser-610-7.pdf", "url": "https://authors.library.caltech.edu/records/jqv96-s0y31/files/J_Cell_Biol-1985-Bronner-Fraser-610-7.pdf" }, "resource_type": "article", "pub_year": "1985", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/b4yjw-b2623", "eprint_id": 66147, "eprint_status": "archive", "datestamp": "2023-08-19 17:40:24", "lastmod": "2023-10-18 17:12:01", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Effects of Different Fragments of the Fibronectin Molecule on Latex Bead Translocation along Neural Crest Migratory Pathways", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1985 Academic Press Inc. \n\nReceived June 4, 1984; accepted in revised farm October 1, 1984. \n\nI thank Dr. Scott Fraser and Dr. Erkki Ruoslahti for critical reading of the manuscript and for helpful suggestions. In addition I gratefully acknowledge the technical assistance of Georgia Guillory and Leena Carriere, and Virginia Bayer for her illustrations. This work was supported by U.S. Public Health Service Grant HD-15527-04 and by Basic Research Grant 1-896 from the March of Dimes Birth Defects Foundation.", "abstract": "Previous studies from this laboratory have utilized latex beads as probes of embryonic migratory pathways. After microinjection into embryos at the time of neural crest migration, uncoated latex polystyrene beads were found to translocate to ventral sites and to settle in the vicinity of endogenous neural crest derivatives. However, latex beads coated with fibronectin did not translocate ventrally, but remained associated with cells surrounding the implantation site. Fibronectin is a large glycoprotein with a variety of biological activities and multiple binding domains. Here, the binding activities which might be responsible for immobilization of the fibronectin-coated beads are examined. Latex beads were coated with three types of fragments of the fibronectin molecule representing different functional domains: (i) a 66-kDa fragment containing collagen-binding activity; (ii) a mixture of 45- and 32-kDa fragments containing heparin-binding activity; and (iii) a 120-kDa fragment containing cell-binding activity. The beads coated with fibronectin fragments were injected into the newly formed trunk somites of avian embryos. After injection, beads coated with either the heparin- or the collagen-binding domain translocated ventrally and distributed analogously to uncoated latex beads. In contrast, the majority of beads coated with the fibronectin cell-binding domain did not translocate but remained associated with dermamyotomal cells surrounding the injection site. The cell-binding fragment, however, was not as effective as the intact fibronectin molecule in preventing translocation of the beads. The results suggest that the cell-binding domain is primarily responsible for restriction of fibronectin beads from the ventral neural crest pathway. Because intact fibronectin is more effective at immobilizing beads than is the cell-binding fragment, other binding domains of fibronectin, more efficient coating with intact fibronectin, or crosslinking of intact fibronectin molecules may also play some role in immobilization of the beads at the implantation site.", "date": "1985-03", "date_type": "published", "publication": "Developmental Biology", "volume": "108", "number": "1", "publisher": "Elsevier", "pagerange": "131-145", "id_number": "CaltechAUTHORS:20160414-080345720", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160414-080345720", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-04" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "1-896" } ] }, "doi": "10.1016/0012-1606(85)90015-6", "resource_type": "article", "pub_year": "1985", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/23g8w-27d05", "eprint_id": 66090, "eprint_status": "archive", "datestamp": "2023-08-19 17:13:50", "lastmod": "2023-10-18 17:08:13", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Coulombe-J-N", "name": { "family": "Coulombe", "given": "James N." } }, { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Translocation of Latex Beads after Laser Ablation of the Avian Neural Crest", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1984 Academic Press, Inc. \n\nReceived February 7, 1984; accepted in revised form June 15, 1984. \n\nWe thank Dr. Scott Fraser for his careful reading of the manuscript, Georgia Guillory and Leena Carriere for their technical assistance, Virginia Bayer for illustrations, and Dirk van Dyke for design of the circuit linking the motorized stage to the laser Q switch. We are grateful to Dr. Michael Berns and the Laser Microbeam Program of the NIH (RR-01192-01) where these experiments were performed.\nThis work was supported by US Public Health Service Grant HD-15527-01 and by Basil O'Connor Starter Research Grant 5-312 from the March of Dimes Birth Defects Foundation.", "abstract": "Previous studies from this laboratory (M. E. Bronner-Fraser, 1982, Dev. Biol. 91, 50\u201363) have demonstrated that latex beads translocate ventrally after injection into avian embryos during the phase of neural crest migration, to settle in the vicinity of neural-crest-derived structures. In order to examine the role of host neural crest cells in the ventral translocation of implanted beads, latex beads have been injected into regions of embryos from which the neural crest cells have been ablated using a laser microbeam. Prior to their migratory phase, neural crest cells reside in the dorsal portion of the neural tube. Laser irradiation of the dorsal neural tube was used to reproducibly achieve either partial or complete ablation of neural crest cells from the irradiated regions. The effectiveness of the ablation was assessed by the degree of reduction in dorsal root ganglia, a neural crest derivative. Because of the rapidity and precision of this technique, it was possible to selectively remove neural crest cells without significantly altering other embryonic structures. The results indicate that, after injection of latex beads into the somites of embryos whose neural crest cells were removed by laser irradiation, the beads translocate ventrally in the absence of the endogenous neural crest.", "date": "1984-11", "date_type": "published", "publication": "Developmental Biology", "volume": "106", "number": "1", "publisher": "Elsevier", "pagerange": "121-134", "id_number": "CaltechAUTHORS:20160412-143132806", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160412-143132806", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH", "grant_number": "RR-01192-01" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "5-312" } ] }, "doi": "10.1016/0012-1606(84)90068-X", "resource_type": "article", "pub_year": "1984", "author_list": "Coulombe, James N. and Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/k7g0y-bhj26", "eprint_id": 67097, "eprint_status": "archive", "datestamp": "2023-08-19 16:58:46", "lastmod": "2023-10-18 20:05:21", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1984 Rockefeller University Press. The Authors acknowledge that RUP will make the Article freely available to the public on RUP's website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central's requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. \n\nReceived for publication 11 February 1983, and in revised form 13\nFebruary 1984. \n\nI thank Dr. Scott Fraser for his helpful criticism of the manuscript and Georgia Guillory and Leena Carriere for their technical assistance. In addition, I gratefully acknowledge the aid of Marthe Howard in performing the electrophoresis experiments and Peggy Garrett for helping with the scanning electron microscopy. This work was supported by U.S. Public Health Service grant HD-15527-01 and by Basil O'Connor Starter Research grant 5-312 from the March of Dimes Birth Defects Foundation.\n\nPublished - J_Cell_Biol-1984-Bronner-Fraser-1947-60.pdf
", "abstract": "In the trunk region of avian embryos, neural crest cells migrate along two pathways: dorsally just under the ectoderm, and ventrally between the neural tube and the somites. Previous work from this laboratory has shown that uncoated latex beads are able to translocate along the ventral neural crest pathway after injection into young embryos; however, beads coated with fibronectin are restricted from the ventral route ( Bronner -Fraser, M.E., 1982, Dev. Biol., 91: 50-63). Here, we extend these observations to determine the effects of other macromolecules on bead distribution. The data show that laminin-coated beads, like fibronectin-coated beads, are restricted from the ventral pathway. In contrast, beads coated with type I collagen translocate ventrally after injection. Because macromolecules have characteristic charge properties, changes in surface charge caused by coating the beads may confound interpretation of the results. Electrostatic effects on bead movement were examined by coating the latex beads with polyamino acids in order to predictably alter the initial surface charge. The surface charge before injection was measured for beads coated with amino acid polymers or with various biologically important macromolecules; the subsequent translocation ability of these beads was then monitored in the embryo. Polylysine-coated beads (positively charged) were restricted from the ventral pathway as were fibronectin and laminin-coated beads, even though fibronectin and laminin beads were both negatively charged. In contrast, polytyrosine -coated beads ( neutrally charged) translocated ventrally as did negatively charged collagen-coated or uncoated beads. The results demonstrate that no correlation exists between the charge properties on the latex bead surface and their subsequent ability to translocate along the ventral pathway. Therefore, an adhesion mechanism independent of surface charge effects must explain the restriction or translocation of latex beads on a neural crest pathway.", "date": "1984-06-01", "date_type": "published", "publication": "Journal of Cell Biology", "volume": "98", "number": "6", "publisher": "Rockefeller University Press", "pagerange": "1947-1960", "id_number": "CaltechAUTHORS:20160516-082837341", "issn": "0021-9525", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160516-082837341", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "5-312" } ] }, "doi": "10.1083/jcb.98.6.1947", "pmcid": "PMC2113074", "primary_object": { "basename": "J_Cell_Biol-1984-Bronner-Fraser-1947-60.pdf", "url": "https://authors.library.caltech.edu/records/k7g0y-bhj26/files/J_Cell_Biol-1984-Bronner-Fraser-1947-60.pdf" }, "resource_type": "article", "pub_year": "1984", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/bbvbp-78w90", "eprint_id": 66116, "eprint_status": "archive", "datestamp": "2023-08-19 15:02:33", "lastmod": "2023-10-18 17:09:56", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Distribution of Latex Beads and Retinal Pigment Epithelial Cells along the Ventral Neural Crest Pathway", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1982 Academic Press, Inc. \n\nReceived October 19, 1981; accepted in revised form December 11, 1981. \n\nI would like to thank Drs. S. E. Fraser and A. Warner for helpful suggestions regarding the manuscript, and Leena Carriere for her technical assistance. This research was supported by U. S. Public Health Service Grant HD-15527-01 and by Basil O'Connor Strater Research Grant 5-312 from the March of Dimes Birth Defects Foundation awarded to M. Bronner-Fraser.", "abstract": "Neural crest cells migrate along defined pathways in the trunk of avian embryos. Previous studies have demonstrated that crest-derived pigment cells migrated ventrally after injection onto the ventral neural crest pathway (M. E. Bronner-Fraser and A. M. Cohen, 1980, Develop. Biol. 77, 130\u2013141). In the present study, latex polystyrene beads and retinal pigment epithelial (RPE) cells were injected onto the ventral pathway in order to probe the environment along this migratory route. Although the RPE cells are nonmotile and not derived from the neural crest, they also translocated ventrally. Thus, factors independent of active migration may affect the localization of RPE cells (and endogenous crest cells). To test this possibility, latex polystyrene beads were injected onto the ventral pathway. Three types of beads were used: (a) uncoated latex beads; (b) latex beads coated with bovine serum albumin (BSA-beads); and (c) latex beads coated with fibronectin (FN-beads). Uncoated and BSA-beads distributed along the ventral pathway similarly to RPE cells and endogenous crest cells. However, FN-beads remained near the site of implantation and did not move ventrally. The results suggest (1) that molecules like fibronectin on the cell surface might serve as a recognition mechanism that prevents entrance onto the ventral pathway; and (2) that crest cell localization may, in part, be influenced by a driving force imparted by the embryonic environment.", "date": "1982-05", "date_type": "published", "publication": "Developmental Biology", "volume": "91", "number": "1", "publisher": "Elsevier", "pagerange": "50-63", "id_number": "CaltechAUTHORS:20160413-111515282", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160413-111515282", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-15527-01" }, { "agency": "March of Dimes Birth Defects Foundation", "grant_number": "5-312" } ] }, "doi": "10.1016/0012-1606(82)90007-0", "resource_type": "article", "pub_year": "1982", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/za42p-y7q71", "eprint_id": 63287, "eprint_status": "archive", "datestamp": "2023-08-19 14:43:42", "lastmod": "2023-10-25 23:42:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" } ] }, "title": "Analysis of neural crest migration and differentiation using a microinjection technique", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1982 International Journal of Neurology.", "abstract": "[no abstract]", "date": "1982", "date_type": "published", "publication": "International Journal of Neurology", "volume": "16-17", "publisher": "International Journal of Neurology", "pagerange": "73-94", "id_number": "CaltechAUTHORS:20160101-142459246", "issn": "0020-7446", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-142459246", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "resource_type": "article", "pub_year": "1982", "author_list": "Bronner-Fraser, Marianne" }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/w03f2-6k119", "eprint_id": 63286, "eprint_status": "archive", "datestamp": "2023-08-19 13:29:15", "lastmod": "2023-10-25 23:42:14", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Sieber-Blum-M", "name": { "family": "Sieber-Blum", "given": "Maya" } }, { "id": "Cohen-A-M", "name": { "family": "Cohen", "given": "Alan M." } } ] }, "title": "Clonal analysis of the avian neural crest: Migration and maturation of mixed neural crest clones injected into host chicken embryos", "ispublished": "pub", "full_text_status": "public", "note": "\u00a9 1980 Alan R. Liss, Inc. \n\nThis research was supported by U.S. Public Health Service grant HD-07389 and a Basic Research Grant from the National Foundation - March of Dimes awarded to A.M.C., by NIH Research Service Award HD05646 to M.S.-B., and by a U.S. Public Health Service Predoctoral Training Grant 5T32GM07231 to M.B.-F.", "abstract": "Quail neural crest cells were grown in vitro at clonal density for 7 or 10 days. Mixed neural crest colonies and clones (containing both pigmented and unpigmented cells) were implanted into the trunk region of 2 1/2-day-old host chicken embryos by a previously described injection technique (Bronner and Cohen, '79). Here we describe the migratory behavior and subsequent phenotypic expression of the injected cells. Unpigmented cells and pigmented cells both migrated along the ventral neural crest pathway; there were, however, some differences in migratory behavior between the two cell types. After 3 days in vivo, unpigmented quail neural crest cells contributed to the sympathetic ganglion, adrenal medulla, and/or aortic plexus in the host. Many of the unpigmented cells became catecholamine-containing neuroblasts. Unpigmented cells were never observed in the gonads or the gut, but localized only in regions normally populated by trunk neural crest precursors to neurons and supportive cells. Melanocytes derived from the same precursor, however, were often found in the gonads or gut, in addition to normal neural crest locations in the trunk. These results demonstrate that quail neural crest cells grown in tissue culture for 7 days or more retain the ability to migrate and contribute to normal neural crest structures when placed in the embryonic environment. Under the conditions described, a single neural crest cell gave rise to daughter cells expressing the melanotic phenotype (detected in tissue culture) and adrenergic phenotypes (detected after injection in vivo). This demonstrates that at least some single cells of the premigratory crest in the trunk region are pluripotent.", "date": "1980-09-15", "date_type": "published", "publication": "Journal of Comparative Neurology", "volume": "193", "number": "2", "publisher": "Wiley-Liss", "pagerange": "423-434", "id_number": "CaltechAUTHORS:20160101-142438234", "issn": "0021-9967", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-142438234", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-07389" }, { "agency": "National Foundation - March of Dimes" }, { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-05646" }, { "agency": "NIH Predoctoral Fellowship", "grant_number": "5T32GM07231" } ] }, "doi": "10.1002/cne.901930209", "resource_type": "article", "pub_year": "1980", "author_list": "Bronner-Fraser, Marianne; Sieber-Blum, Maya; et el." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/jqka1-ccb27", "eprint_id": 66120, "eprint_status": "archive", "datestamp": "2023-08-19 13:18:45", "lastmod": "2023-10-18 17:10:16", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne" }, "orcid": "0000-0003-4274-1862" }, { "id": "Cohen-A-M", "name": { "family": "Cohen", "given": "Alan M." } } ] }, "title": "Analysis of the Neural Crest Ventral Pathway Using Injected Tracer Cells", "ispublished": "pub", "full_text_status": "restricted", "note": "\u00a9 1980 Academic Press, Inc. \n\nReceived May 23, 1979; accepted in revised form November 28, 1979. \n\nWe thank Dr. S. E. Fraser for advice in developing the injection technique, and Ms. Susan Jaffe and Ms. Wanda Wicks for expert technical assistance. This research was supported by U.S. Public Health Service Grant HD-07389, NIH Biomedical Research Support Grant RR-05378, and a Basic Research Grant from the National Foundation-March of Dimes, awarded to Alan M. Cohen.", "abstract": "Cloned neural crest melanocytes and nonneural crest cells were introduced into chick embryos to study questions of neural crest migration and localization. Previously we showed that cloned quail melanocytes injected directly into the newly formed somites of 2.5-day-old chick embryos were initially contained within the somitic lumen (Bronner and Cohen, 1979); following release from the somites, the melanocytes migrated along the ventral neural crest pathway. Here we demonstrate that the migratory pattern of melanocytes freshly isolated from the skin of 9- to 11-day-old quail embryos is identical to that of the cloned quail melanocytes grownin vitro for 8 days or more. Like cloned melanocytes, quail skin melanocytes migrated beyond the dorsal root and sympathetic chain ganglia and localized in the vicinity of the adrenal gland, aortic plexus, metanephric mesenchyme, gonads, and gut. This suggests that the choice of the ventral migratory route is not simply a consequence of prolonged stay in tissue culture. Nor is the choice of the ventral pathway a consequence of introducing cells of one species into the environment of another since melanocytes derived from chick neural crest cells migrated analogously to the quail melanocytes. The ventral pathway appears to be selective for neural crest-derived cells since somite cells and single fibroblast cells did not migrate ventrally when placed into the embryo. Quail somite cells remained associated with the somitic mesenchyme and single fibroblast cells migrated under the ectoderm. Injected quail fibroblasts when clumped, however, were found on the ventral pathway indicating that the ventral route is not specific for crest cells and their derivatives. Cloned quail melanocytes injected into older developmental levels of the neural axis did not migrate as far ventrally as those implanted in younger levels where crest cells were only beginning to migrate. This demonstrates that changes in the environment that occur as a function of developmental age limit the extent of ventral migration of injected melanocytes.", "date": "1980-06-01", "date_type": "published", "publication": "Developmental Biology", "volume": "77", "number": "1", "publisher": "Elsevier", "pagerange": "130-141", "id_number": "CaltechAUTHORS:20160413-111516447", "issn": "0012-1606", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160413-111516447", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-07389" }, { "agency": "NIH", "grant_number": "RR-05378" }, { "agency": "National Foundation - March of Dimes" } ] }, "doi": "10.1016/0012-1606(80)90461-3", "resource_type": "article", "pub_year": "1980", "author_list": "Bronner-Fraser, Marianne and Cohen, Alan M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/2danf-nsg18", "eprint_id": 63285, "eprint_status": "archive", "datestamp": "2023-08-19 12:55:16", "lastmod": "2024-01-13 16:30:31", "type": "book_section", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner-Fraser", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Cohen-A-M", "name": { "family": "Cohen", "given": "Alan M." } } ] }, "title": "The neural crest: what can it tell us about cell migration and determination?", "ispublished": "unpub", "full_text_status": "public", "note": "\u00a9 1980 Academic Press. \n\nWe express our gratitude to Ms. Susan Jaffe for her expert technical assistance. The research was supported by U.S. Public Health Service Grant HD-07389 and A Basic Research Grant from the National Foundation-March of Dimes.", "abstract": "Of all vertebrate structures, the nervous system has perhaps the greatest cellular diversification and complexity of organization. The study of developmental neurobiology, therefore, sharply probes the central issue of embryogenesis; i.e., how does a complex system arise form a single cell? During development, ectodermal tissue becomes determined along neuronal lines via contact or \"primary induction\" from the roof of the archenteron (Mangold, 1933; Spemann, 1938). A major problem is how the morphologically indistinguishable stem cells of the neuroectoderm become progressively determined along divergent developmental lines such that they give rise to a myriad of cell types in the mature nervous system. Another question posed by this system is how the cells migrate to their final locations and become organized in precise spatial arrays. Once the pattern is composed, what mechanisms govern the establishment of the proper connections between neurons and other neurons, as well as between neurons and their target organs?", "date": "1980", "date_type": "published", "publisher": "Academic Press", "place_of_pub": "New York, NY", "pagerange": "1-25", "id_number": "CaltechAUTHORS:20160101-141827472", "isbn": "0-12-153115-5", "book_title": "Neural Development Part I: Emergence of Specificity in Neural Histogenesis", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160101-141827472", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-07389" }, { "agency": "National Foundation - March of Dimes" } ] }, "contributors": { "items": [ { "id": "Hunt-R-K", "name": { "family": "Hunt", "given": "R. Kevin" } } ] }, "resource_type": "book_section", "pub_year": "1980", "author_list": "Bronner-Fraser, Marianne E. and Cohen, Alan M." }, { "id": "https://authors.library.caltech.eduhttps://authors.library.caltech.edu/records/12z3s-1sn51", "eprint_id": 64103, "eprint_status": "archive", "datestamp": "2023-08-22 01:59:59", "lastmod": "2023-10-17 19:30:20", "type": "article", "metadata_visibility": "show", "creators": { "items": [ { "id": "Bronner-M-E", "name": { "family": "Bronner", "given": "Marianne E." }, "orcid": "0000-0003-4274-1862" }, { "id": "Cohen-A-M", "name": { "family": "Cohen", "given": "Alan M." } } ] }, "title": "Migratory patterns of cloned neural crest melanocytes injected into host chicken embryos", "ispublished": "pub", "full_text_status": "public", "keywords": "migration; pigment cells; injection technique; differentiation", "note": "\u00a9 1979 National Academy of Sciences. \n\nCommunicated by David Bodian, January 15, 1979. \n\nThe research was supported by U.S. Public Health Service Grant HD-07389 and a Basic Research Grant from The National Foundation-March of Dimes awarded to A.M.C. and U.S. Public Health Service Predoctoral Training Grant 5T32GM07231 to M.E.B. \n\nThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked \"advertisement\" in accordance with 18 U. S. C. \u00a71734 solely to indicate\nthis fact.\n\nPublished - pnas00004-0325.pdf
", "abstract": "Cloned quail melanocytes grown in tissue culture for 8 days or more were injected into 2 1/2-day-old chicken embryos. The pigment cells were placed directly into the somitic lumen by means of an injection micropipette. This technique for introducing marked neural crest cells into host embryos causes far less damage than previous methods which require extirpation and replacement of the neural tube. In addition, small numbers of homogeneous cells can be implanted by this procedure. When injected into one of the posterior somites, cultured pigment cells migrated along the ventral neural crest pathway. Three days after injection the melanocytes had migrated ventral to the dorsal root ganglia and prevertebral and primary sympathetic chain ganglia and were seen associated with the adrenal gland and aortic plexi. Melanocytes were frequently found in or adjacent to the gonads and often had migrated as far as the gut.", "date": "1979-04", "date_type": "published", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "volume": "76", "number": "4", "publisher": "National Academy of Sciences", "pagerange": "1843-1847", "id_number": "CaltechAUTHORS:20160129-144855016", "issn": "0027-8424", "official_url": "https://resolver.caltech.edu/CaltechAUTHORS:20160129-144855016", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "U.S. Public Health Service (USPHS)", "grant_number": "HD-07389" }, { "agency": "National March of Dimes" }, { "agency": "NIH Predoctoral Fellowship", "grant_number": "5T32GM07231" } ] }, "pmcid": "PMC383488", "primary_object": { "basename": "pnas00004-0325.pdf", "url": "https://authors.library.caltech.edu/records/12z3s-1sn51/files/pnas00004-0325.pdf" }, "resource_type": "article", "pub_year": "1979", "author_list": "Bronner, Marianne E. and Cohen, Alan M." } ]