[ { "id": "authors:0ygdx-afm92", "collection": "authors", "collection_id": "0ygdx-afm92", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151214-155200160", "type": "article", "title": "The production of nitric oxide in endothelial cells by amphiphiles", "author": [ { "family_name": "Conforto", "given_name": "A.", "clpid": "Conforto-A" }, { "family_name": "Dudek", "given_name": "R.", "clpid": "Dudek-R" }, { "family_name": "Hoffmann", "given_name": "M. R.", "orcid": "0000-0001-6495-1946", "clpid": "Hoffmann-M-R" }, { "family_name": "Bing", "given_name": "R. J.", "clpid": "Bing-R-J" } ], "abstract": "Lysophosphatidylcholine, an endogenous detergent is an endothelium-dependent smooth muscle relaxant, which acts through the release of nitric oxide. It is known to activate a number of membrane-bound enzymes. Because of the relationship between detergent action, relaxation of endothelium-intact rabbit aortic strips and the release of nitric oxide, we considered the possibility that other amphiphiles also produce nitric oxide from endothelial cells. We therefore investigated the effect of digitonin on relaxation of precontracted rabbit aortic strips and the release of nitric oxide from freshly harvested bovine endothelial cells as determined by chemiluminescence. We found that both digitonin and LPC release nitric oxide and that this process is inhibited by the NO synthase inhibitor N\u03c9-Nitro-L-Arginine Methyl Ester (L-NNAME).", "doi": "10.1016/0024-3205(94)00836-1", "issn": "0024-3205", "publisher": "Elsevier", "publication": "Life Sciences", "publication_date": "1994", "series_number": "16", "volume": "54", "issue": "16", "pages": "1143-1153" }, { "id": "authors:kk6nj-9sw91", "collection": "authors", "collection_id": "kk6nj-9sw91", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151214-155602204", "type": "article", "title": "Membrane function and vascular reactivity", "author": [ { "family_name": "Bing", "given_name": "R. J.", "clpid": "Bing-R-J" }, { "family_name": "Termin", "given_name": "A.", "clpid": "Termin-A" }, { "family_name": "Conforto", "given_name": "A.", "clpid": "Conforto-A" }, { "family_name": "Dudek", "given_name": "R.", "clpid": "Dudek-R" }, { "family_name": "Hoffmann", "given_name": "M. J.", "orcid": "0000-0001-6495-1946", "clpid": "Hoffmann-M-R" } ], "abstract": "This communication examines the possibility that nitric oxide (NO) production by endothelial cells results from changes in cell membrane fluidity. Lysophosphatidylcholine (LPC) alters fluidity of the endothelial cell membranes causing vascular relaxation. Through membrane alterations LPC influences function of a number of membrane receptors and modulates enzyme activity. As a result of detergent action, lysophosphatidylcholine (LPC) causes activation of guanylate cyclase, stimulates syalytransferase and regulates protein kinase C activity. It has already been demonstrated that ionic detergents, such as Triton X-100 also cause vascular relaxation, possibly induced by NO production from endothelial cells. It is postulated that production of nitric oxide results from changes in membrane viscosity; this may represent a mechanism for its regulation in biological systems.", "doi": "10.1007/BF01145958", "issn": "0144-8463", "publisher": "Portland Press", "publication": "Bioscience Reports", "publication_date": "1993-04-01", "series_number": "2", "volume": "13", "issue": "2", "pages": "61-67" }, { "id": "authors:qx9am-f4297", "collection": "authors", "collection_id": "qx9am-f4297", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:SAIpnas88", "type": "article", "title": "Lysolecithins as Endothelium-Dependent Vascular Smooth Muscle Relaxants that Differ from Endothelium-Derived Relaxing Factor (Nitric Oxide)", "author": [ { "family_name": "Saito", "given_name": "Takashi", "clpid": "Saito-Takashi" }, { "family_name": "Wolf", "given_name": "Andreas", "clpid": "Wolf-Andreas" }, { "family_name": "Menon", "given_name": "Nirmala K.", "clpid": "Menon-N-K" }, { "family_name": "Saeed", "given_name": "Maythem", "clpid": "Saeed-M" }, { "family_name": "Bing", "given_name": "Richard J.", "clpid": "Bing-R-J" } ], "abstract": "The effects of lysolecithin (lysophosphatidylcholine) derived from egg yolk as well as of synthetic lysolecithins with different aliphatic chain lengths on tension development of rabbit aortic strips were investigated. Lysolecithins caused slowly progressing, dose-dependent relaxation that was inhibited by hemoglobin, methylene blue, and nordihydroguiaretic acid. Indomethacin caused no inhibition of relaxation. The degree of relaxation was endothelium-dependent and appeared to be related to the activation of guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2]. Superoxide dismutase failed to influence relaxation. Lysolecithins with the longest aliphatic chain were the most potent relaxants of aortic strips. The experiments suggest a role of lysolecithins through their weak detergent action on membrane dynamics of endothelial cells, resulting in the production of cyclic GMP and the relaxation of arterial smooth muscle. Lysolecithins differ in several respects from endothelium-derived relaxing factor. Endothelium-derived relaxing factor is an unstable humoral substance released from endothelium and is identical to nitric oxide, itself a labile substance causing vascular relaxation and cyclic GMP accumulation. Lysolecithins may represent a different type of endothelium-dependent muscle relaxant.", "doi": "10.1073/pnas.85.21.8246", "pmcid": "PMC282406", "issn": "0027-8424", "publisher": "National Academy of Sciences", "publication": "Proceedings of the National Academy of Sciences of the United States of America", "publication_date": "1988-11-01", "series_number": "21", "volume": "85", "issue": "21", "pages": "8246-8250" }, { "id": "authors:c2zrv-31053", "collection": "authors", "collection_id": "c2zrv-31053", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20151002-123937515", "type": "article", "title": "Advantages of perfluorochemical perfusion in the isolated working rabbit heart preparation using ^(31)P-NMR", "author": [ { "family_name": "Freeman", "given_name": "Dominique", "clpid": "Freeman-D" }, { "family_name": "Mayr", "given_name": "H.", "clpid": "Mayr-H" }, { "family_name": "Schmidt", "given_name": "Paul.", "clpid": "Schmidt-P" }, { "family_name": "Roberts", "given_name": "John D.", "clpid": "Roberts-J-D" }, { "family_name": "Bing", "given_name": "Richard J.", "clpid": "Bing-R-J" } ], "abstract": "Quantitative ^(31)P-NMR and enzymatic analysis of high-energy phosphates were used to characterize an isolated perfused working rabbit heart preparation. In this model, the left side of the heart works against a physiological after-load. Two perfusates, Krebs-Henseleit saline and the perfluorocarbon emulsion FC-43 (perfluorotributylamine), were evaluated in their ability to maintain cardiac function and high-energy phosphate metabolites over a period of 2\u20133 h. Adenine nucleotides ATP, ADP, phosphocreatine and inorganic phosphate (P_i) were measured by ^(31)P-NMR while monitoring cardiac output and coronary flow. Intracellular pH was determined using the chemical shift of P_i. At the end of each experiment, hearts were freeze clamped and enzymatically assayed for adenine nucleotides, phosphocreatine and P_i. In every experiment, hearts perfused with FC-43 emulsion maintained the same rate of cardiac output as hearts perfused with Krebs-Henseleit saline, but with half the coronary flow rate: FC-43, 22 \u00b1 2.5 (n = 5), Krebs-Henseleit saline 42 \u00b1 2.7 (n = 6) ml/min, P < 0.001. Hearts perfused with FC-43 emulsion showed higher [phosphocreatine] and [ATP] measured by 31P-NMR. For [phosphocreatine]: FC-43 3.2 \u00b1 0.7 (n = 5), Krebs-Henseleit saline 1.7 \u00b1 0.2 (n = 6) \u03bcmol/g wet wt., P < 0.01. For [ATP]: FC-43 1.8 \u00b1 0.7 (n = 5), Krebs-Henseleit saline 0.9 \u00b1 0.2 (n = 6) \u03bcmol/g wet wt., P < 0.02. [phosphocreatine] and [ATP] determined by ^(31)P-NMR values were identical within experimental error to those values obtained by enzymatic analysis. Comparing [P_i] determined by both methods, 36% of P_i in FC-43-perfused hearts, and only 24% of P_i in Krebs-Henseleit saline-perfused hearts were visible by NMR, indicating that a large proportion of P_i is bound in the intact functioning heart. Similar results were obtained for [ADP]. Using the combined techniques of ^(31)P-NMR and enzymatic assay, we have shown in this model of the isolated working rabbit heart preparation, that FC-43 emulsion maintains significantly better function and high-energy phosphate levels than Krebs-Henseleit saline.", "doi": "10.1016/0167-4889(87)90099-1", "issn": "0167-4889", "publisher": "Elsevier", "publication": "Biochimica et Biophysica Acta - Molecular Cell Research", "publication_date": "1987-03-11", "series_number": "3", "volume": "927", "issue": "3", "pages": "350-358" }, { "id": "authors:j550h-bc248", "collection": "authors", "collection_id": "j550h-bc248", "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20221004-680171300.9", "type": "article", "title": "Use of the fast Fourier transform in the frequency analysis of the second heart sound in normal man", "author": [ { "family_name": "Yoganathan", "given_name": "Ajit P.", "clpid": "Yoganathan-Ajit-P" }, { "family_name": "Gupta", "given_name": "Ramesh", "clpid": "Gupta-Ramesh" }, { "family_name": "Corcoran", "given_name": "William H.", "clpid": "Corcoran-W-H" }, { "family_name": "Udwadia", "given_name": "Firdaus E.", "orcid": "0000-0003-1055-4165", "clpid": "Udwadia-Firdaus-E" }, { "family_name": "Sarma", "given_name": "Radha", "clpid": "Sarma-Radha" }, { "family_name": "Bing", "given_name": "Richard J.", "clpid": "Bing-R-J" } ], "abstract": "The second heart sounds of 26 normal, young males, recorded at the aortic and pulmonary areas, were analysed for their frequency content by means of the fast Fourier transform. For both locations of measurement, peaks were observed in the frequency spectra in the lowfrequency range (10\u201380 Hz), the medium-frequency range (80\u2013220 Hz), and the high-frequency range (220\u2013400 Hz). In both the aortic and pulmonary areas, 25 of the 26 subjects had at least two peaks in the 80\u2013400 Hz range, and a majority had one peak in the low-frequency range. A correlation coefficient of 0\u00b775 was obtained between the medium frequency peaks in the aortic and pulmonary areas. The average frequency spectrum obtained for the entire study at each area indicates that the attenuation characteristics are nonlinear in the region of 10\u2013160 Hz. For 160\u2013400 Hz the attenuation in the pulmonary area is about 18 dB per octave and in the aortic area about 23 dB per octave. The observed peaks are probably related to the fluid-dynamic events causing the second heart sound. Thus important diagnostic information can probably be obtained from frequency analysis studies of cardiovascular sounds, and these studies will help in understanding the basic mechanisms which produce the sounds.", "doi": "10.1007/bf02476124", "issn": "0025-696X", "publisher": "Springer", "publication": "Medical and Biological Engineering", "publication_date": "1976-07", "series_number": "4", "volume": "14", "issue": "4", "pages": "455-460" } ]