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"metadata_visibility": "show", "creators": { "items": [ { "email": "gil@caltech.edu", "id": "Carvalho-Gil-Bastos-de", "name": { "family": "Carvalho", "given": "Gil Bastos de" }, "show_email": "NO" } ] }, "title": "Drosophila Feeding Behavior and Demographic Mechanisms of Lifespan Extension", "ispublished": "unpub", "full_text_status": "public", "keywords": "Drosophila, aging, nutrition, lifespan", "abstract": "Why do we age? Most organisms undergo senescence, a process involving progressive functional decline culminating in death, yet this widespread phenomenon remains largely mysterious. A number of genetic and environmental factors affect longevity, the best conserved and most widely studied of which is dietary restriction (DR), a reduction of nutrient intake short of malnutrition. Since nutrient ingestion determines lifespan, any factor affecting longevity\u2014particular food components, genetic pathways or drugs\u2014may do so indirectly, by altering feeding behavior. This is particularly true in Drosophila, which is normally kept in conditions where food is present in excess. Moreover, since DR is applied by aging flies on two different food concentrations\u2014diluted media are associated with an extended lifespan\u2014animals may alter their intake in response to the change in nutrient content. Since the medium is also the only water source, this compensatory feeding would result in changes in hydration, introducing a second experimental variable. Despite these issues, Drosophila feeding behavior has classically been ignored or superficially characterized in the context of aging research, partly due to the absence of appropriate methodology. We developed two complementary assays allowing long-term measurement of food intake. Using these techniques, we present the first characterization of real-time Drosophila feeding behavior. Our results reveal gender-specific feeding trends and show that mating stimulates female appetite via the seminal Sex Peptide. Additionally, we show that ingestion is dramatically affected by food dilution or dietary additives. Animals fed concentrated media restrict their intake and are chronically thirsty. We have found that lifespan extension by classical DR paradigms is abolished in the presence of ad libitum water, challenging the long-held assumption that DR affects longevity by altering nutrient intake. We characterize a new regime that robustly prolongs lifespan irrespective of water availability, and thus likely represents a more relevant model for mammalian DR. In contrast to previous claims, demographic analysis using this paradigm indicates that DR acts not by reducing the immediate risk of death, but by slowing the accumulation of age-related damage. Our findings directly challenge current views on the mechanistic basis of DR and have broad implications for the study of aging and nutrition in model organisms. \r\n\r\n\r\n", "date": "2010", "date_type": "degree", "id_number": "CaltechTHESIS:06022010-183440587", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:06022010-183440587", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechTHESIS", "reviewer": "Gayle Hammer", "deposited_by": "Gil Carvalho", "deposited_on": "2011-06-06 16:02:37", "doi": "10.7907/NMN1-N566", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "email": "wuwei@caltech.edu", "id": "Anderson-D-J", "name": { "family": "Anderson", "given": "David J." }, "role": "advisor" }, { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "email": "wuwei@caltech.edu", "id": "Anderson-D-J", "name": { "family": "Anderson", "given": "David J." }, "role": "chair" }, { "email": "deshaies@caltech.edu", "id": "Deshaies-R-J", "name": { "family": "Deshaies", "given": "Raymond Joseph" }, "role": "member" }, { "email": "pws@caltech.edu", "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "role": "member" }, { "email": "baltimo@caltech.edu", "id": "Baltimore-D-L", "name": { "family": "Baltimore", "given": "David L." }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "2010-02-01", "gradofc_approval_date": "2011-06-03", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "2010", "author_list": "Carvalho, Gil Bastos de", "advisor_list": "Anderson, David J. and Benzer, Seymour", "comittee_list": "Anderson, David J.; Deshaies, Raymond Joseph; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/5189", "eprint_id": 5189, "rev_number": 18, "documents": [ { "id": "/id/document/8216", "doc_id": 8216, "rev_number": 2, "files": [ { "id": "/id/file/50753", "fileid": 50753, "datasetid": "document", "objectid": 8216, "filename": "thesis-final-JulienMuffat2009.pdf", "mime_type": "application/pdf", "filesize": 3132581, "mtime": "2012-12-26 03:15:48", "url": "/5189/1/thesis-final-JulienMuffat2009.pdf" } ], "eprint_id": 5189, "pos": 1, "mime_type": "application/pdf", "format": "application/pdf", "format_desc": "thesis-final-JulienMuffat2009.pdf", "language": "en", "security": 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"indexcodes.txt", "media_duration": "0", "media_aspect_ratio": "0", "media_sample_start": "0", "media_sample_stop": "0", "relation": { "items": [ { "type": "http://eprints.org/relation/isVersionOf", "uri": "/id/document/12209" }, { "type": "http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/12209" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/12209" } ] } }, { "id": "/id/document/124917", "doc_id": 124917, "rev_number": 1, "files": [ { "id": "/id/file/370347", "fileid": 370347, "datasetid": "document", "objectid": 124917, "filename": "indexcodes.txt", "mime_type": "text/plain", "hash": "b52bf08adad475349031d45fa6e61de8", "hash_type": "MD5", "filesize": 818, "mtime": "2021-06-23 23:58:04", "url": "/5189/7/indexcodes.txt" } ], "eprint_id": 5189, "pos": 7, "placement": 7, "mime_type": "text/plain", "format": "other", "format_desc": "Generate index codes conversion from text/plain to indexcodes", "language": "en", "security": "internal", "license": "other", "main": "indexcodes.txt", "media_duration": "0", "media_aspect_ratio": "0", "media_sample_start": "0", "media_sample_stop": "0", "relation": { "items": [ { "type": "http://eprints.org/relation/isVersionOf", "uri": "/id/document/12212" }, { "type": "http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/12212" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/12212" } ] } } ], "eprint_status": "archive", "userid": 2, "dir": "disk0/00/00/51/89", "datestamp": "2009-05-29", "lastmod": "2019-11-26 19:46:13", "status_changed": "2011-04-04 19:11:08", "type": "thesis", "metadata_visibility": "show", "creators": { "items": [ { "email": "julien@wi.mit.edu", "id": "Muffat-J-A", "name": { "family": "Muffat", "given": "Julien A." }, "orcid": "0000-0003-1889-7023", "show_email": "YES" } ] }, "title": "Role of Apolipoprotein D and Its Homologs, in Normal and Pathological Aging, in Drosophila melanogaster", "ispublished": "unpub", "full_text_status": "public", "keywords": "aging; alzheimer's disease; neurodegeneration; oxidative stress", "abstract": "The free radical theory of aging is probably the most enduring one to date. It stipulates that, in the process of normal cellular function, in particular due to oxygen-based respiration, reactive oxygen species are formed. These constantly put a strain on the cell, damaging lipids in the membranes, causing protein aggregation and loss-of-function, or mutations in the genome. Over time, this accumulated damage overcomes the repair potential of a given cell, and scaled up to an entire organism, results in the deterioration seen in normal aging. Under these assumptions, age-related pathologies are only an acceleration of the process in a given tissue, leading to the emergence of the pathology over the noise of normal aging. In the past decade, invertebrates such as Drosophila melanogaster and C. elegans have provided invaluable insight into these processes and the canonical pathways that regulate them. This success is owed in part to the power of the genetic tools available, to their relatively short lifespans, and to the wide arrays of phenotypes that can be studied. We set out to study a particular protein, Glaz, whose overexpression enables fruitflies to live 30% longer than normal. This protein was identified as a hit from a screen looking at an accelerated aging paradigm, placing fruitflies in 100% oxygen. While an interesting protein in its own right, it was through its homology with mammalian Apolipoprotein D (ApoD), that our interest was truly piqued. ApoD and its homologs turn out to be fascinating proteins, upregulated by various stresses and in age-related diseases such as cancers and Alzheimer\u2019s. We showed, in our model organism of choice, that this upregulation is part of a beneficial stress response. Understanding and harnessing its functions can only help provide therapeutic approaches for a wide range of disorders.\r\n", "date": "2009", "date_type": "degree", "id_number": "CaltechETD:etd-05182009-232337", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechETD:etd-05182009-232337", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechTHESIS", "reviewer": "Kathy Johnson", "deposited_by": "Imported from ETD-db", "deposited_on": "2009-05-29", "doi": "10.7907/H1ST-ZY74", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" }, { "email": "php@calteh.edu", "id": "Patterson-P-H", "name": { "family": "Patterson", "given": "Paul H." }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "email": "php@calteh.edu", "id": "Patterson-P-H", "name": { "family": "Patterson", "given": "Paul H." }, "role": "chair" }, { "email": "avarsh@caltech.edu", "id": "Varshavsky-A-J", "name": { "family": "Varshavsky", "given": "Alexander J." }, "role": "member" }, { "email": "haybruce@caltech.edu", "id": "Hay-B-A", "name": { "family": "Hay", "given": "Bruce A." }, "role": "member" }, { "email": "davidwalker@ucla.edu", "id": "Walker-D", "name": { "family": "Walker", "given": "David" }, "role": "member" }, { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_submitted_date": "2009-05-18", "thesis_defense_date": "2008-12-17", "thesis_approved_date": "2009-05-29", "gradofc_approval_date": "2009-05-29", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive\nlicense to archive and make accessible, under the conditions specified below,\nmy thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. 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"thesis", "metadata_visibility": "show", "creators": { "items": [ { "email": "bzid@ucsd.edu", "id": "Zid-Brian-Matthew", "name": { "family": "Zid", "given": "Brian Matthew" }, "orcid": "0000-0003-1876-2479", "show_email": "NO" } ] }, "title": "Translational Control Mediates Lifespan Extension Due to Dietary Restriction in Drosophila", "ispublished": "unpub", "full_text_status": "public", "keywords": "aging; dietary restriction; translation", "abstract": "Aging is characterized by the declining ability of an organism to maintain homeostasis, which eventually leads to death. Dietary restriction (DR), the reduction of nutrients without malnutrition, extends lifespan in various organisms, yet its molecular underpinnings are poorly understood. We show that in Drosophila, DR upregulates the translational repressor 4EBP, the eukaryotic translation initiation factor 4E binding protein, and that this upregulation is necessary for the full lifespan extension upon DR and sufficient to extend lifespan on a nutrient rich diet. Investigation of the genome-wide translational changes upon DR using translation state array analysis (TSAA) found that translationally downregulated genes tend to have extensive 5\u2019 untranslated regions (UTR) secondary structures, while those that are upregulated have weakly structured 5\u2019UTRs. Among the translationally upregulated genes, mitochondrial ribosomal proteins and electron transport chain components were overrepresented. Mitochondrial genes were found to have weakly structured 5\u2019UTRs in Drosophila, and this was conserved in Humans. The 5\u2019UTRs of mitochondrial genes were found to be sufficient to confer preferential translation during times of high 4EBP activity in a cap-independent manner to reporter constructs. Upregulation of mitochondrial function was verified and found to be d4EBP dependent, implicating a novel mechanism for regulating mitochondrial function upon DR. These results implicate mRNA translation initiation in modulating lifespan and mitochondrial function upon DR.", "date": "2008", "date_type": "degree", "id_number": "CaltechETD:etd-02052008-020618", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechETD:etd-02052008-020618", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechTHESIS", "reviewer": "Kathy Johnson", "deposited_by": "Imported from ETD-db", "deposited_on": "2008-02-21", "doi": "10.7907/8DZY-WB19", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "email": "jcampbel@caltech.edu", "id": "Campbell-J-L", "name": { "family": "Campbell", "given": "Judith L." }, "role": "co-chair" }, { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "co-chair" }, { "email": "avarsh@caltech.edu", "id": "Varshavsky-A-J", "name": { "family": "Varshavsky", "given": "Alexander J." }, "role": "member" }, { "email": "pws@caltech.edu", "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "role": "member" }, { "email": "deshaies@caltech.edu", "id": "Deshaies-R-J", "name": { "family": "Deshaies", "given": "Raymond Joseph" }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_submitted_date": "2008-02-05", "thesis_defense_date": "2008-01-17", "thesis_approved_date": "2008-02-21", "gradofc_approval_date": "2008-02-21", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive\nlicense to archive and make accessible, under the conditions specified below,\nmy thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "2008", "author_list": "Zid, Brian Matthew", "advisor_list": "Benzer, Seymour", "comittee_list": "Campbell, Judith L.; Benzer, Seymour; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/11872", "eprint_id": 11872, "rev_number": 28, "documents": [ { "id": "/id/document/103962", "doc_id": 103962, "rev_number": 3, "files": [ { "id": "/id/file/310049", "fileid": 310049, "datasetid": "document", "objectid": 103962, "filename": "Schwarz_EM_1995.pdf", "mime_type": "application/pdf", "hash": "4fc0b57b81e37321737bad8f78f0086e", "hash_type": "MD5", "filesize": 66239, "mtime": "2019-10-29 21:57:10", "url": "/11872/6/Schwarz_EM_1995.pdf" } ], "eprint_id": 11872, "pos": 6, "placement": 6, "mime_type": "application/pdf", "format": "application/pdf", "format_desc": "author ok 10/29/2019", "language": "en", 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"indexcodes.txt", "media_duration": "0", "media_aspect_ratio": "0", "media_sample_start": "0", "media_sample_stop": "0", "relation": { "items": [ { "type": "http://eprints.org/relation/isVersionOf", "uri": "/id/document/103986" }, { "type": "http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/103986" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/103986" } ] } }, { "id": "/id/document/132611", "doc_id": 132611, "rev_number": 1, "files": [ { "id": "/id/file/378056", "fileid": 378056, "datasetid": "document", "objectid": 132611, "filename": "indexcodes.txt", "mime_type": "text/plain", "hash": "83d79fcb23bf14fdcae07ddb72d50522", "hash_type": "MD5", "filesize": 579, "mtime": "2021-06-24 19:02:05", "url": "/11872/18/indexcodes.txt" } ], "eprint_id": 11872, "pos": 18, "placement": 18, "mime_type": "text/plain", "format": "other", "format_desc": "Generate index codes conversion from application/pdf to indexcodes", "language": "en", "security": "internal", "license": "other", "main": "indexcodes.txt", "media_duration": "0", "media_aspect_ratio": "0", "media_sample_start": "0", "media_sample_stop": "0", "relation": { "items": [ { "type": "http://eprints.org/relation/isVersionOf", "uri": "/id/document/103962" }, { "type": "http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/103962" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/103962" } ] } } ], "eprint_status": "archive", "userid": 8820, "dir": "disk0/00/01/18/72", "datestamp": "2019-10-29 20:46:07", "lastmod": "2019-12-21 03:05:08", "status_changed": "2019-10-29 20:46:07", "type": "thesis", "metadata_visibility": "show", "creators": { "items": [ { "email": "ems394@cornell.edu", "id": "Schwarz-Erich-Marquard", "name": { "family": "Schwarz", "given": "Erich Marquard" }, "orcid": "0000-0003-3151-4381", "show_email": "YES" } ] }, "title": "Calx, A Sodium-Calcium Exchanger of Drosophila melanogaster", "ispublished": "unpub", "full_text_status": "public", "keywords": "Calx, Na-Ca exchanger, sodium-calcium exchanger, Drosophila melanogaster", "abstract": "
Calcium extrusion is necessary for cellular survival and suspected to modulate cellular activity. Drosophila phototransduction is a promising system in which to study calcium export, since it is dominated by calcium activity yet, unlike most calcium-dependent signalling pathways, genetically pliable. The multiple roles of calcium flux in Drosophila phototransduction are reviewed in Chapter One.
\r\n\r\nCalx, a Drosophila ortholog of mammalian 3Na+/1Ca2+ exchangers, was isolated and characterized (Chapter Two). Calx's gene product has ~50% identity to its direct mammalian homologs, with more distant similarities to an exchanger-related superfamily. There exist at least seven alternately spliced adult Calx transcripts, with an alternatively spliced miniexon in Calx's protein-coding region. A full-length Calx cDNA of 5408 bp has lengthy, elaborate 5' and 3' UTRs. Calx transcripts are ubiquitously expressed in embryos and adult heads, with one 5.7 kb transcript expressed in photoreceptors; Calx protein is also ubiquitous in adult heads, with a notable presence in photoreceptors and neuropil. Heterologous expression of Calx in Xenopus oocytes shows that it encodes a bona fide sodium-calcium exchanger; unlike mammalian retinal exchangers, it does not depend on potassium for activity. Calx encodes two novel protein motifs, Calx-\u03b1 and Calx-\u03b2. Both are intragenically duplicated, but they probably have different functions: Calx-\u03b1 is likely to encode residues central to calcium export, while Calx-\u03b2 may mediate intracellular signalling or cytoskeletal anchoring.
\r\n\r\n", "date": "1996", "date_type": "degree", "id_number": "CaltechTHESIS:10292019-132931068", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:10292019-132931068", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechTHESIS", "reviewer": "Melissa Ray", "other_numbering_system": { "items": [ { "id": "9611527", "name": "UMI" } ] }, "suggestions": "Downloaded from UMI", "deposited_by": "Mel Ray", "deposited_on": "2019-10-29 20:46:07", "doi": "10.7907/M2PR-WT51", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "chair" }, { "email": "wuwei@caltech.edu", "id": "Anderson-D-J", "name": { "family": "Anderson", "given": "David J." }, "role": "member" }, { "email": "meyerow@caltech.edu", "id": "Meyerowitz-E-M", "name": { "family": "Meyerowitz", "given": "Elliot M." }, "role": "member" }, { "email": "simonm@caltech.edu", "id": "Simon-M-I", "name": { "family": "Simon", "given": "Melvin I." }, "role": "member" }, { "email": "pws@caltech.edu", "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "1995-08-21", "review_status": "approved", "option_major": { "items": [ "molbio" ] }, "option_minor": { "items": [ "bioch" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "1996", "author_list": "Schwarz, Erich Marquard", "advisor_list": "Benzer, Seymour", "comittee_list": "Benzer, Seymour; Anderson, David J.; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/7684", "eprint_id": 7684, "rev_number": 14, "documents": [ { "id": "/id/document/38087", "doc_id": 38087, "rev_number": 3, "files": [ { "id": "/id/file/118193", "fileid": 118193, "datasetid": "document", "objectid": 38087, "filename": "Leiserson 1994.zip", "mime_type": "application/x-zip", "hash": "b294bd6612bfbc3b1188675344beeefe", "hash_type": "MD5", "filesize": 82786879, "mtime": "2013-05-08 23:25:29", "url": "/7684/1/Leiserson 1994.zip" } ], "eprint_id": 7684, "pos": 1, "placement": 1, "mime_type": "application/x-zip", "format": "application/zip", "format_desc": "TIFF", "language": "en", "security": 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"http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/38094" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/38094" } ] } } ], "eprint_status": "archive", "userid": 50, "dir": "disk0/00/00/76/84", "datestamp": "2013-05-08 23:45:47", "lastmod": "2022-11-09 19:20:00", "status_changed": "2013-05-08 23:45:47", "type": "thesis", "metadata_visibility": "show", "creators": { "items": [ { "id": "Leiserson-W-M", "name": { "family": "Leiserson", "given": "William M." }, "show_email": "NO" } ] }, "title": "Molecular genetics of the Drosophila eyes absent gene", "ispublished": "unpub", "full_text_status": "public", "keywords": "Biology", "abstract": "The Drosophila compound eye has provided a genetic approach to\r\nunderstanding the specification of cell fates during differentiation. The eye is\r\nmade up of some 750 repeated units or ommatidia, arranged in a lattice. The\r\ncellular composition of each ommatidium is identical. The arrangement of the\r\nlattice and the specification of cell fates in each ommatidium are thought to occur\r\nin development through cellular interactions with the local environment. Many\r\nmutations have been studied that disrupt the proper patterning and cell fating in the\r\neye. The eyes absent (eya) mutation, the subject of this thesis, was chosen because\r\nof its eyeless phenotype. In eya mutants, eye progenitor cells undergo\r\nprogrammed cell death before the onset of patterning has occurred. The molecular\r\ngenetic analysis of the gene is presented.
\r\n\r\nThe eye arises from the larval eye-antennal imaginal disc. During the third\r\nlarval instar, a wave of differentiation progresses across the disc, marked by a\r\nfurrow. Anterior to the furrow, proliferating cells are found in apparent disarray.\r\nPosterior to the furrow, clusters of differentiating cells can be discerned, that\r\ncorrespond to the ommatidia of the adult eye. Analysis of an allelic series of eya\r\nmutants in comparison to wild type revealed the presence of a selection point: a\r\nwave of programmed cell death that normally precedes the furrow. In eya\r\nmutants, an excessive number of eye progenitor cells die at this selection point,\r\nsuggesting the eya gene influences the distribution of cells between fates of death\r\nand differentiation.
\r\n\r\nIn addition to its role in the eye, the eya gene has an embryonic function.\r\nThe eye function is autonomous to the eye progenitor cells. Molecular maps of the\r\neye and embryonic phenotypes are different. Therefore, the function of eya in the\r\neye can be treated independently of the embryonic function. Cloning of the gene reveals two cDNA's that are identical except for the use of an alternatively-spliced\r\n5' exon. The predicted protein products differ only at the N-termini. Sequence\r\nanalysis shows these two proteins to be the first of their kind to be isolated.\r\nTrangenic studies using the two cDNA's show that either gene product is able to\r\nrescue the eye phenotype of eya mutants.
\r\n\r\nThe eya gene exhibits interallelic complementation. This interaction is an\r\nexample of an \"allelic position effect\": an interaction that depends on the relative\r\nposition in the genome of the two alleles, which is thought to be mediated by\r\nchromosomal pairing. The interaction at eya is essentially identical to a\r\nphenomenon known as transvection, which is an allelic position effect that is\r\nsensitive to certain kinds of chromosomal rearrangements. A current model for\r\nthe mechanism of transvection is the trans action of gene regulatory regions. The\r\neya locus is particularly well suited for the study of transvection because the\r\nmutant phenotypes can be quantified by scoring the size of the eye.
\r\n\r\nThe molecular genetic analysis of eya provides a system for uncovering\r\nmechanisms underlying differentiation, developmentally regulated programmed\r\ncell death, and gene regulation.
\r\n", "date": "1994", "date_type": "degree", "id_number": "CaltechTHESIS:05082013-155756716", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:05082013-155756716", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "collection": "CaltechTHESIS", "reviewer": "Tony Diaz", "deposited_on": "2013-05-08 23:45:47", "doi": "10.7907/3jn4-xk56", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "id": "Anderson-D-J", "name": { "family": "Anderson", "given": "David J." }, "role": "member" }, { "id": "Lipshitz-H-D", "name": { "family": "Lipshitz", "given": "Howard D." }, "role": "member" }, { "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "1993-12-20", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "1994", "author_list": "Leiserson, William M.", "advisor_list": "Benzer, Seymour", "comittee_list": "Anderson, David J.; Lipshitz, Howard D.; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/7944", "eprint_id": 7944, "rev_number": 26, "documents": [ { "id": "/id/document/42941", "doc_id": 42941, "rev_number": 3, "files": [ { "id": "/id/file/128242", "fileid": 128242, "datasetid": "document", "objectid": 42941, "filename": "Renfranz 1989.pdf", "mime_type": "application/pdf", "hash": "9edf8323086562fd0a27c1221eeb2198", "hash_type": "MD5", "filesize": 37048783, "mtime": "2013-08-26 21:09:43", "url": "/7944/1/Renfranz 1989.pdf" } ], "eprint_id": 7944, "pos": 1, "placement": 1, "mime_type": "application/pdf", "format": "application/pdf", "language": "en", "security": "public", "license": "other", 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"0", "media_sample_stop": "0", "relation": { "items": [ { "type": "http://eprints.org/relation/isVersionOf", "uri": "/id/document/42941" }, { "type": "http://eprints.org/relation/isVolatileVersionOf", "uri": "/id/document/42941" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/42941" } ] } }, { "id": "/id/document/135021", "doc_id": 135021, "rev_number": 3, "files": [ { "id": "/id/file/383772", "fileid": 383772, "datasetid": "document", "objectid": 135021, "filename": "renfranz-pj_1989.zip", "mime_type": "application/zip", "hash": "450b7bb5f9fbeeeb1028ee2717b6a490", "hash_type": "MD5", "filesize": 158972998, "mtime": "2021-09-30 00:56:26", "url": "/7944/8/renfranz-pj_1989.zip" } ], "eprint_id": 7944, "pos": 8, "placement": 8, "mime_type": "application/zip", "format": "application/zip", "format_desc": "TIFFs", "language": "en", "security": "internal", "license": "other", "main": "renfranz-pj_1989.zip", "media_duration": "0", "media_aspect_ratio": "0", "media_sample_start": "0", "media_sample_stop": "0", "content": "archival" } ], "eprint_status": "archive", "userid": 50, "dir": "disk0/00/00/79/44", "datestamp": "2013-08-26 21:33:21", "lastmod": "2021-09-30 00:56:28", "status_changed": "2013-08-26 21:33:21", "type": "thesis", "metadata_visibility": "show", "creators": { "items": [ { "id": "Renfranz-Patricia-Jean", "name": { "family": "Renfranz", "given": "Patricia Jean" }, "show_email": "NO" } ] }, "title": "Molecular Neurogenetics of Eye Development in Drosophila", "ispublished": "unpub", "full_text_status": "public", "keywords": "Biology", "abstract": "The compound eye of Drosophila melanogaster begins to differentiate during the late third larval instar in the eye-antennal imaginal disc. A wave of morphogenesis crosses the disc from posterior to anterior, leaving behind precisely patterned clusters of photoreceptor cells and accessory cells that will constitute the adult ommatidia of the retina. By the analysis of genetically mosaic eyes, it appears that any cell in the eye disc can adopt the characteristics of any one of the different cell types found in the mature eye, including photoreceptor cells and non-neuronal accessory cells such as cone cells. Therefore, cells within the prospective retinal epithelium assume different fates presumably via information present in the environment. The sevenless\u207a (sev\u207a) gene appears to play a role in the expression of one of the possible fates, since the mutant phenotype is the lack of one of the pattern elements, namely, photoreceptor cell R7. The sev\u207a gene product had been shown to be required during development of the eye, and had also been shown in genetic mosaics to be autonomous to presumptive R7. As a means of better understanding the pathway instructing the differentiation R7, the gene and its protein product were characterized.
\r\n\r\nThe sev\u207a gene was cloned by P-element transposon tagging, and was found to encode an 8.2 kb transcript expressed in developing eye discs and adult heads. By raising monoclonal antibodies (MAbs) against a sev\u207a-\u03b2-galactosidase fusion protein, the expression of the protein in the eye disc was localized by immuno-electronmicroscopy. The protein localizes to the apical cell membranes and microvilli of cells in the eye disc epithelium. It appears during development at a time coincident with the initial formation of clusters, and in all the developing photoreceptors and accessory cone cells at a time prior to the overt differentiation of R7. This result is consistent with the pluripotency of cells in the eye disc. Its localization in the membranes suggests that it may receive information directing the development of R7. Its localization in the apical membranes and microvilli is away from the bulk of the cell contacts, which have been cited as a likely regions for information presentation and processing. Biochemical characterization of the sev\u207a protein will be necessary to describe further its role in development.
\r\n\r\nOther mutations in Drosophila have eye phenotypes. These were analyzed to find which ones affected the initial patterning of cells in the eye disc, in order to identify other genes, like sev, whose gene products may be involved in generating the pattern. The adult eye phenotypes ranged from severe reduction of the eye, to variable numbers of photoreceptor cells per ommatidium, to sub de defects in the organization of the supporting cells. Developing eye discs from the different strains were screened using a panel of MAbs, which highlight various developmental stages. Two identified matrix elements in and anterior to the furrow, while others identified the developing ommatidia themselves, like the anti-sev MAb. Mutation phenotypes were shown to appear at many stages of development. Some mutations seem to affect the precursor cells, others, the setting up of the pattern, and still others, the maintenance of the pattern. Thus, additional genes have now been identified that may function to support the development of a complex pattern.
", "date": "1989", "date_type": "degree", "id_number": "CaltechTHESIS:08262013-140100846", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:08262013-140100846", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "NSF" }, { "agency": "Lawrence A. Hanson Foundation" } ] }, "collection": "CaltechTHESIS", "reviewer": "Tony Diaz", "deposited_on": "2013-08-26 21:33:21", "doi": "10.7907/15zs-fh27", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "chair" }, { "email": "wuwei@caltech.edu", "id": "Anderson-D-J", "name": { "family": "Anderson", "given": "David J." }, "orcid": "0000-0001-6175-3872", "role": "member" }, { "email": "meyerow@caltech.edu", "id": "Meyerowitz-E-M", "name": { "family": "Meyerowitz", "given": "Elliot M." }, "orcid": "0000-0003-4798-5153", "role": "member" }, { "email": "simonm@caltech.edu", "id": "Simon-M-I", "name": { "family": "Simon", "given": "Melvin I." }, "role": "member" }, { "email": "pws@caltech.edu", "id": "Sternberg-P-W", "name": { "family": "Sternberg", "given": "Paul W." }, "orcid": "0000-0002-7699-0173", "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "1988-12-15", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "1989", "author_list": "Renfranz, Patricia Jean", "advisor_list": "Benzer, Seymour", "comittee_list": "Benzer, Seymour; Anderson, David J.; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/10906", "eprint_id": 10906, "rev_number": 20, "documents": [ { "id": "/id/document/89037", "doc_id": 89037, "rev_number": 3, "files": [ { "id": "/id/file/252461", "fileid": 252461, "datasetid": "document", "objectid": 89037, "filename": "Shotwell_SL_1982.pdf", "mime_type": "application/pdf", "hash": "30413613cd172ea6a7dad33145e305ab", "hash_type": "MD5", "filesize": 41193889, "mtime": "2018-05-17 00:59:41", "url": "/10906/1/Shotwell_SL_1982.pdf" } ], "eprint_id": 10906, "pos": 1, "placement": 1, "mime_type": "application/pdf", "format": "application/pdf", "language": "en", "security": "public", "license": 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"uri": "/id/document/89037" }, { "type": "http://eprints.org/relation/isIndexCodesVersionOf", "uri": "/id/document/89037" } ] } } ], "eprint_status": "archive", "userid": 8820, "dir": "disk0/00/01/09/06", "datestamp": "2018-07-02 17:42:41", "lastmod": "2022-08-08 17:58:22", "status_changed": "2018-07-02 17:42:41", "type": "thesis", "metadata_visibility": "show", "creators": { "items": [ { "id": "Shotwell-Sandra-Lee", "name": { "family": "Shotwell", "given": "Sandra Lee" }, "show_email": "NO" } ] }, "title": "A Biochemical and Genetic Analysis of the Cyclic AMP Phosphodiesterase Defect in dunce, a Memory Mutant of Drosophila", "ispublished": "unpub", "full_text_status": "public", "keywords": "Biology", "abstract": "Drosophila can learn in several associative conditioning paradigms. Flies carrying the mutation dunce were selected for their poor performance in one such task, a negative reinforcement olfactory conditioning paradigm (Dudai et al., 1976). dunce flies express two other mutant phenotypes, female sterility, and reduced activity for one of the two cyclic AMP phosphodiesterases present in normal flies, PDE II (Byers et al., 1981) . . The experiments described below indicate that the normal dunce gene (dunce+) probably codes for PDE II itself, rather than for a regulator that affects PDE II and possibly other activities.
\r\n\r\nA micro-assay technique is described that allows the separate measurement of PDE I and PDE II when both are present in mixture. PDE II is shown to occur at high specific activity in the nervous system, which is consistent with a role for this enzyme in neuronal function. The phenotype of female sterility associated with dunce mutants can be suppressed by any of three suppressor mutations. These do not suppress the other two phenotypes of reduced PDE II activity and poor learning, indicating that these phenotypes are closer to the primary defect associated with dunce mutants. Reduced PDE II activity correlates with poor learning in dunce flies in all three developmental stages that were tested (first and third instar larvae, and adults), as well as in response to genetic modifications of dunce gene activity. The results of several biochemical and genetic experiments fail to reveal any abnormal regulation of PDE II activity in dunce flies. In Drosophila, as a rule, the activity level of an enzyme correlates linearly with the activity of the enzyme's structural gene. The specific activity of PDE II is shown to correlate in a one to one fashion with the level of normal dunce gene activity at five different doses of dunce+.
\r\n\r\nTaken as a whole, these experiments provide strong support for the hypothesis that PDE II represents the primary product of the dunce gene, indicating a role for this enzyme in the learning of Drosophila.
", "date": "1982", "date_type": "degree", "id_number": "CaltechTHESIS:05162018-173145200", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:05162018-173145200", "related_url": { "items": [ { "description": "Article adapted for appendix.", "type": "doi", "url": "https://doi.org/10.1111/j.1749-6632.1981.tb30854.x" } ] }, "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NIH" }, { "agency": "Lawrence E. Hanson Foundation" }, { "agency": "Jean Weigle Memorial Fund" } ] }, "collection": "CaltechTHESIS", "reviewer": "Tony Diaz", "deposited_by": "Mel Ray", "deposited_on": "2018-07-02 17:42:41", "doi": "10.7907/67ye-6j88", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "id": "Brockes-Jeremy-P", "name": { "family": "Brockes", "given": "Jeremy P." }, "role": "chair" }, { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "member" }, { "id": "Hudspeth-J", "name": { "family": "Hudspeth", "given": "James" }, "role": "member" }, { "email": "kennedym@its.caltech.edu", "id": "Kennedy-M-B", "name": { "family": "Kennedy", "given": "Mary B." }, "orcid": "0000-0003-1369-0525", "role": "member" }, { "id": "Mitchell-H-K", "name": { "family": "Mitchell", "given": "Herschel K." }, "role": "member" }, { "id": "Owen-R-D", "name": { "family": "Owen", "given": "Ray David" }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "1982-05-20", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "1982", "author_list": "Shotwell, Sandra Lee", "advisor_list": "Benzer, Seymour", "comittee_list": "Brockes, Jeremy P.; Benzer, Seymour; et el." }, { "id": "https://thesis.library.caltech.edu/id/eprint/9639", "eprint_id": 9639, "rev_number": 15, "documents": [ { "id": "/id/document/64475", "doc_id": 64475, "rev_number": 2, "files": [ { "id": "/id/file/180449", "fileid": 180449, "datasetid": "document", "objectid": 64475, "filename": "Konopka_rj_1972.pdf", "mime_type": "application/pdf", "hash": "e6da3482a0deef45c9e7c4be1985c1c6", "hash_type": "MD5", "filesize": 25109976, "mtime": "2016-04-01 19:29:15", "url": "/9639/1/Konopka_rj_1972.pdf" } ], "eprint_id": 9639, "pos": 1, "placement": 1, "mime_type": "application/pdf", "format": "application/pdf", "language": "en", "security": "public", "license": "other", 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One mutant is arrhythmic, another has a short period of 19 hours, and the third has a long period of 28 hours. The mutants retain their phenotypes over the temperature range 18\u00b0 to 25\u00b0 C. All three mutants map near the tip of the X chromosome (distal to the centromere). By deficiency mapping, the short-period mutation has been localized to the 3B1-2 region. Complementation tests show that all three mutations affect the same functional gene.
\r\n\r\nAnalysis of activity rhythms of individual mosaic flies indicates that the site of action of the short-period mutation is probably located in the head of the fly. A few activity patterns of split-head and mixed-head mosaics appear to possess both mutant and heterozygous components, suggesting that the fly head may contain\r\ntwo complete clocks capable of maintaining their periodicities independently.
\r\n\r\nThe short-period mutation affects both the duration of the light-insensitive part of the oscillation and the degree to which the clock can be reset during the light-sensitive part of the oscillation.
\r\n\r\nBoth the short-period and long-period mutant eclosion rhythms can be entrained to a period of 24 hours by a 12:12 light-dark cycle having a light intensity at least two orders of magnitude greater than that required to entrain the normal rhythm. The arrhythmic mutant does not entrain under these conditions. In the presence of a temperature cycle, however, the arrhythmic mutant does entrain, but its rhythm damps out when the temperature cycle is removed.
\r\n\r\nEvidence is presented that Pittendrigh's two-oscillator model for the clock in D. pseudoobscura applies to D. melanogaster as well. The three clock mutations primarily affect the light- sensitive driving oscillator. The arrhythmic mutation appears to have eliminated the driving oscillator while leaving the temperature-sensitive driven oscillator relatively intact.
\r\n", "date": "1972", "date_type": "degree", "id_number": "CaltechTHESIS:04012016-120503899", "refereed": "FALSE", "official_url": "https://resolver.caltech.edu/CaltechTHESIS:04012016-120503899", "rights": "No commercial reproduction, distribution, display or performance rights in this work are provided.", "funders": { "items": [ { "agency": "NSF" }, { "agency": "Caltech" } ] }, "collection": "CaltechTHESIS", "reviewer": "Tony Diaz", "deposited_on": "2016-04-01 20:14:08", "doi": "10.7907/R04B-3425", "divisions": { "items": [ "div_biol" ] }, "institution": "California Institute of Technology", "thesis_type": "phd", "thesis_advisor": { "items": [ { "id": "Benzer-S", "name": { "family": "Benzer", "given": "Seymour" }, "role": "advisor" } ] }, "thesis_committee": { "items": [ { "id": "Lewis-E-B", "name": { "family": "Lewis", "given": "Edward B." }, "role": "member" }, { "id": "Mitchell-H-K", "name": { "family": "Mitchell", "given": "Herschel K." }, "role": "member" }, { "id": "Strumwasser-F", "name": { "family": "Strumwasser", "given": "Felix" }, "role": "member" }, { "id": "Vinograd-J", "name": { "family": "Vinograd", "given": "Jerome" }, "role": "member" } ] }, "thesis_degree": "PHD", "thesis_degree_grantor": "California Institute of Technology", "thesis_defense_date": "1972-02-29", "review_status": "approved", "option_major": { "items": [ "biology" ] }, "copyright_statement": "Author's Rights Authorization: I hereby certify that, if appropriate, I have obtained a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted here is the same as that approved by my advisory committee.\n\nI hereby grant to California Institute of Technology or its agents the non-exclusive license to archive and make accessible, under the conditions specified under \"Thesis Availability\" in this submission, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation, or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.", "resource_type": "thesis", "pub_year": "1972", "author_list": "Konopka, Ronald Jerome", "advisor_list": "Benzer, Seymour", "comittee_list": "Lewis, Edward B.; Mitchell, Herschel K.; et el." } ]