[
    {
        "id": "thesis:8104",
        "collection": "thesis",
        "collection_id": "8104",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:03052014-104605861",
        "primary_object_url": {
            "basename": "Bashirullah_a_1999.pdf",
            "content": "final",
            "filesize": 39924656,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/8104/1/Bashirullah_a_1999.pdf",
            "version": "v3.0.0"
        },
        "type": "thesis",
        "title": "Temporal and spacial control of RNA stability in the early embryo of Drosophila melanogaster",
        "author": [
            {
                "family_name": "Bashirullah",
                "given_name": "Arash",
                "clpid": "Bashirullah-A"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Lipshitz",
                "given_name": "Howard D.",
                "clpid": "Lipshitz-H-D"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Lipshitz",
                "given_name": "Howard D.",
                "clpid": "Lipshitz-H-D"
            },
            {
                "family_name": "Meyerowitz",
                "given_name": "Elliot M.",
                "clpid": "Meyerowitz-E-M"
            },
            {
                "family_name": "Sternberg",
                "given_name": "Paul W.",
                "clpid": "Sternberg-P-W"
            },
            {
                "family_name": "Wold",
                "given_name": "Barbara J.",
                "clpid": "Wold-B-J"
            },
            {
                "family_name": "Zinn",
                "given_name": "Kai George",
                "clpid": "Zinn-K-G"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "Early embryogenesis in metazoa is controlled by maternally synthesized\r\nproducts. Among these products, the mature egg is loaded with transcripts\r\nrepresenting approximately two thirds of the genome. A subset of this maternal\r\nRNA pool is degraded prior to the transition to zygotic control of development.\r\nThis transfer of control of development from maternal to zygotic products is\r\nreferred to as the midblastula transition (or MBT). It is believed that the\r\ndegradation of maternal transcripts is required to terminate maternal control of\r\ndevelopment and to allow zygotic control of development to begin.\r\nUntil now this process of maternal transcript degradation and the subsequent\r\ntiming of the MBT has been poorly understood. I have demonstrated that in the\r\nearly embryo there are two independent RNA degradation pathways, either of\r\nwhich is sufficient for transcript elimination. However, only the concerted action\r\nof both pathways leads to elimination of transcripts with the correct timing, at\r\nthe MBT. The first pathway is maternally encoded, is triggered by egg\r\nactivation, and is targeted to specific classes of mRNAs through cis-acting\r\nelements in the 3' untranslated region (UTR}. The second pathway is activated\r\n2 hr after fertilization and functions together with the maternal pathway to\r\nensure that transcripts are degraded by the MBT. In addition, some transcripts\r\nfail to degrade at select subcellular locations adding an element of spatial\r\ncontrol to RNA degradation. The spatial control of RNA degradation is achieved\r\nby protecting, or masking, transcripts from the degradation machinery. The\r\nRNA degradation and protection events are regulated by distinct cis-elements\r\nin the 3' untranslated region (UTR). These results provide the first systematic\r\ndissection of this highly conserved process in development and demonstrate\r\nthat RNA degradation is a novel mechanism used for both temporal and spatial\r\ncontrol of development.",
        "doi": "10.7907/HWD3-HB90",
        "publication_date": "1999",
        "thesis_type": "phd",
        "thesis_year": "1999"
    }
]