[
    {
        "id": "authors:phyet-39772",
        "collection": "authors",
        "collection_id": "phyet-39772",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20121127-135839836",
        "type": "book_section",
        "title": "MicroRNAs and Hematopoietic Cell Development",
        "book_title": "MicroRNAs in Development",
        "author": [
            {
                "family_name": "O'Connell",
                "given_name": "Ryan M.",
                "clpid": "O'Connell-Ryan-M"
            },
            {
                "family_name": "Baltimore",
                "given_name": "David",
                "orcid": "0000-0001-8723-8190",
                "clpid": "Baltimore-D"
            }
        ],
        "contributor": [
            {
                "family_name": "Hornstein",
                "given_name": "Eran",
                "clpid": "Hornstein-E"
            }
        ],
        "abstract": "Hematopoiesis is a dynamic and highly complex developmental process that gives rise to a multitude of the cell types that circulate in the blood of multicellular organisms. These cells provide tissues with oxygen, guard against infection, prevent bleeding by clotting, and mediate inflammatory reactions. Because the hematopoietic system plays such a central role in human diseases such as infections, cancer, autoimmunity, and anemia, it has been intensely studied for more than a century. This scrutiny has helped to shape many of the developmental paradigms that exist today and has identified specific protein factors that serve as master regulators of blood cell lineage specification. Despite this progress, many aspects of blood cell development remain obscure, suggesting that novel layers of regulation must exist. Consequently, the emergence of regulatory noncoding RNAs, such as the microRNAs (miRNAs), is beginning to provide new insights into the molecular control networks underlying hematopoiesis and diseases that stem from aberrations in this process. This review will discuss how miRNAs fit into our current understanding of hematopoietic development in mammals and how breakdowns in these pathways can trigger disease.",
        "doi": "10.1016/B978-0-12-387038-4.00006-9",
        "isbn": "978-0-12-387038-4",
        "publisher": "Elsevier",
        "place_of_publication": "Amsterdam",
        "publication_date": "2012",
        "pages": "145-174"
    },
    {
        "id": "authors:pcyph-cfh07",
        "collection": "authors",
        "collection_id": "pcyph-cfh07",
        "cite_using_url": "https://resolver.caltech.edu/CaltechAUTHORS:20200512-154625286",
        "type": "book_section",
        "title": "Identification of 3BP-1 in cDNA expression library by SH3 domain screening",
        "book_title": "Small GTPases and Their Regulators Part B: Rho Family",
        "author": [
            {
                "family_name": "Cicchetti",
                "given_name": "Piera",
                "clpid": "Cicchetti-P"
            },
            {
                "family_name": "Baltimore",
                "given_name": "David",
                "orcid": "0000-0001-8723-8190",
                "clpid": "Baltimore-D"
            }
        ],
        "contributor": [
            {
                "family_name": "Hall",
                "given_name": "Alan",
                "clpid": "Hall-Alan"
            },
            {
                "family_name": "Balch",
                "given_name": "W. E.",
                "clpid": "Balch-W-E"
            },
            {
                "family_name": "Der",
                "given_name": "Channing J.",
                "clpid": "Der-C-J"
            }
        ],
        "abstract": "This chapter describes a screening procedure to assay for protein\u2013protein interactions using a biotinylated glutathione S-transferase (GST) fusion protein probe to screen cDNA expression libraries. This method is developed to allow nonradioactive probing, which has the additional advantages of a very low background signal and a low incidence of false positives. Because neither the fusion protein probe nor the protein in the expression library is denatured, this assay allows the structural components of the binding reaction to remain intact. This screening procedure offers a practical and highly efficient method of identifying protein\u2013protein interactions. Using this procedure to detect proteins that bound to the Abl SH3 domain, five cDNA clones were isolated out of 7 million cDNA containing plaques that were screened. Of these five, three contained the identical cDNA clone termed \"3BP-1,\" while the other two were identical for a different clone termed \"3BP-2.\" On sequencing these clones, only a short stretch of approximately 40 amino acids was found where sequence similarity existed between these proteins.",
        "doi": "10.1016/0076-6879(95)56019-x",
        "isbn": "978-0-12-182157-9",
        "publisher": "Academic Press",
        "place_of_publication": "San Diego, CA",
        "publication_date": "1995",
        "pages": "140-148"
    }
]