- Sarai, Nicholas S.; Fulton, Tyler J.; et el. (2024) Directed
evolution of enzymatic silicon-carbon bond cleavage in siloxanes;
Science; Vol. 383; No. 6681; 438-443; 10.1126/science.adi5554
- Rogge, Torben; Zhou, Qingyang; et el. (2024) Iron Heme
Enzyme-Catalyzed Cyclopropanations with Diazirines as Carbene
Precursors: Computational Explorations of Diazirine Activation and
Cyclopropanation Mechanism; Journal of the American Chemical
Society; 10.1021/jacs.3c06030
- Wackelin, Daniel J.; Mao, Runze; et el. (2024) Enzymatic
Assembly of Diverse Lactone Structures: An Intramolecular C–H
Functionalization Strategy; Journal of the American Chemical
Society; 10.1021/jacs.3c11722
- Qin, Zi-Yang; Gao, Shilong; et el. (2023) Biocatalytic
Construction of Chiral Pyrrolidines and Indolines via Intramolecular
C(sp³)–H Amination; ACS Central Science; Vol. 9; No. 12; 2333-2338;
PMCID PMC10755850; 10.1021/acscentsci.3c00516
- Das, Anuvab; Long, Yueming; et el. (2023) Expanding
Biocatalysis for Organosilane Functionalization: Enantioselective
Nitrene Transfer to Benzylic Si–C–H Bonds; ACS Catalysis; Vol. 14;
148-152; 10.1021/acscatal.3c05370
- Mao, Runze; Taylor, Doris Mia; et el. (2023) Biocatalytic,
stereoconvergent alkylation of (Z/E)-trisubstituted silyl enol
ethers; Nature Synthesis; 10.1038/s44160-023-00431-2
- Gao, Shilong; Das, Anuvab; et el. (2023) Enzymatic
Nitrogen Incorporation Using Hydroxylamine; Journal of the American
Chemical Society; Vol. 145; No. 37; 20196-20201; 10.1021/jacs.3c08053
- Mao, Runze; Wackelin, Daniel J.; et el. (2023) Enantio-
and Diastereoenriched Enzymatic Synthesis of 1,2,3-Polysubstituted
Cyclopropanes from (Z/E)-Trisubstituted Enol Acetates; Journal of
the American Chemical Society; Vol. 145; No. 29; 16176-16185; PMCID
PMC10528827; 10.1021/jacs.3c04870
- Danelius, Emma; Porter, Nicholas J.; et el. (2023) MicroED
Structure of a Protoglobin Reactive Carbene Intermediate; Journal of
the American Chemical Society; Vol. 145; No. 13; 7159-7165; PMCID
PMC10080679; 10.1021/jacs.2c12004
- Zhang, Juner; Maggiolo, Ailiena O.; et el. (2023) Chemodivergent
C(sp³)–H and C(sp²)–H cyanomethylation using engineered carbene
transferases; Nature Catalysis; Vol. 6; No. 2; 152-160; PMCID
PMC9983643; 10.1038/s41929-022-00908-x
- Schaus, Lucas; Das, Anuvab; et el. (2023) Protoglobin-Catalyzed
Formation of cis-Trifluoromethyl-Substituted Cyclopropanes by Carbene
Transfer; Angewandte Chemie International Edition; Vol. 62; No. 4;
Art. No. e202208936; PMCID PMC9894577; 10.1002/anie.202208936
- Alfonzo, Edwin; Das, Anuvab; et el. (2022) New
additions to the arsenal of biocatalysts for noncanonical amino acid
synthesis; Current Opinion in Green and Sustainable Chemistry; Vol.
38; Art. No. 100701; PMCID PMC9770695; 10.1016/j.cogsc.2022.100701
- Athavale, Soumitra V.; Gao, Shilong; et el. (2022) Enzymatic
Nitrogen Insertion into Unactivated C-H Bonds; Journal of the
American Chemical Society; Vol. 144; No. 41; 19097-19105; PMCID
PMC9612832; 10.1021/jacs.2c08285
- Porter, Nicholas J.; Danelius, Emma; et el. (2022) Biocatalytic
Carbene Transfer Using Diazirines; Journal of the American Chemical
Society; Vol. 144; No. 20; 8892-8896; PMCID PMC9205183; 10.1021/jacs.2c02723
- Molina, Rosana S.; Rix, Gordon; et el. (2022) In
vivo hypermutation and continuous evolution; Nature Reviews Methods
Primers; Vol. 2; Art. No. 36; 10.1038/s43586-022-00119-5
- Miller, David C.; Lal, Ravi G.; et el. (2022) Biocatalytic
One-Carbon Ring Expansion of Aziridines to Azetidines via a Highly
Enantioselective [1,2]-Stevens Rearrangement; Journal of the
American Chemical Society; Vol. 144; No. 11; 4739-4745; PMCID
PMC9022672; 10.1021/jacs.2c00251
- Wittmann, Bruce J.; Johnston, Kadina E.; et el. (2022) evSeq:
Cost-Effective Amplicon Sequencing of Every Variant in a Protein
Library; ACS Synthetic Biology; Vol. 11; No. 3; 1313-1324; 10.1021/acssynbio.1c00592
- Arnold, Frances H. (2022) Innovation
by evolution: bringing new chemistry to life; Biophysical Journal;
Vol. 121; No. 3; 177a; 10.1016/j.bpj.2021.11.1846
- Liu, Zhen; Qin, Zi-Yang; et el. (2022) An
Enzymatic Platform for Primary Amination of 1-Aryl-2-alkyl Alkynes;
Journal of the American Chemical Society; Vol. 144; No. 1; 80-85; PMCID
PMC8765727; 10.1021/jacs.1c11340
- Miller, David C.; Athavale, Soumitra V.; et el. (2022) Combining
chemistry and protein engineering for new-to-nature biocatalysis;
Nature Synthesis; Vol. 1; No. 1; 18-23; PMCID PMC8995090; 10.1038/s44160-021-00008-x
- Liu, Zhen; Calvó-Tusell, Carla; et el. (2021) Dual-function
enzyme catalysis for enantioselective carbon–nitrogen bond
formation; Nature Chemistry; Vol. 13; No. 12; 1166-1172; 10.1038/s41557-021-00794-z
- Wu, Zachary; Johnston, Kadina E.; et el. (2021) Protein
sequence design with deep generative models; Current Opinion in
Chemical Biology; Vol. 65; 18-27; 10.1016/j.cbpa.2021.04.004
- Wittmann, Bruce J.; Yue, Yisong; et el. (2021) Informed
training set design enables efficient machine learning-assisted directed
protein evolution; Cell Systems; Vol. 12; No. 11; 1026-1045; 10.1016/j.cels.2021.07.008
- Athavale, Soumitra V.; Gao, Shilong; et el. (2021) Biocatalytic,
Intermolecular C−H Bond Functionalization for the Synthesis of
Enantioenriched Amides; Angewandte Chemie International Edition;
Vol. 60; No. 47; 24864-24869; PMCID PMC8578410; 10.1002/anie.202110873
- Watkins-Dulaney, Ella J.; Dunham, Noah P.; et el. (2021) Asymmetric
Alkylation of Ketones Catalyzed by Engineered TrpB; Angewandte
Chemie International Edition; Vol. 60; No. 39; 21412-21417; PMCID
PMC8440449; 10.1002/anie.202106938
- Wittmann, Bruce J.; Johnston, Kadina E; et el. (2021) Advances
in machine learning for directed evolution; Current Opinion in
Structural Biology; Vol. 69; 11-18; 10.1016/j.sbi.2021.01.008
- Arnold, Frances (2021) Innovation
by evolution: bringing new chemistry to life; Acta Crystallographica
Section A; Vol. A77; No. Supplement; Art. No. a70; 10.1107/S0108767321099293
- Sarai, Nicholas S.; Levin, Benjamin J.; et el. (2021) Biocatalytic
Transformations of Silicon—the Other Group 14 Element; ACS Central
Science; Vol. 7; No. 6; 944-953; PMCID PMC8227617; 10.1021/acscentsci.1c00182
- Liu, Zhen and Arnold, Frances H. (2021) New-to-nature
chemistry from old protein machinery: carbene and nitrene
transferases; Current Opinion in Biotechnology; Vol. 69; 43-51;
PMCID PMC8225731; 10.1016/j.copbio.2020.12.005
- Garcia-Borràs, Marc; Kan, S. B. Jennifer; et el. (2021) Origin
and Control of Chemoselectivity in Cytochrome c Catalyzed Carbene
Transfer into Si–H and N–H bonds; Journal of the American Chemical
Society; Vol. 143; No. 18; 7114-7123; PMCID PMC9292473; 10.1021/jacs.1c02146
- Yang, Yang and Arnold, Frances H. (2021) Navigating
the Unnatural Reaction Space: Directed Evolution of Heme Proteins for
Selective Carbene and Nitrene Transfer; Accounts of Chemical
Research; Vol. 54; No. 5; 1209-1225; PMCID PMC7931446; 10.1021/acs.accounts.0c00591
- Watkins-Dulaney, Ella; Straathof, Sabine; et el. (2021) Tryptophan
Synthase: Biocatalyst Extraordinaire; ChemBioChem; Vol. 22; No. 1;
5-16; PMCID PMC7935429; 10.1002/cbic.202000379
- Pluchinsky, Adam J.; Wackelin, Daniel J.; et el. (2020) High
Throughput Screening with SAMDI Mass Spectrometry for Directed
Evolution; Journal of the American Chemical Society; Vol. 142;
No. 47; 19804-19808; 10.1021/jacs.0c07828
- Rix, Gordon; Watkins-Dulaney, Ella J.; et el. (2020) Scalable
continuous evolution for the generation of diverse enzyme variants
encompassing promiscuous activities; Nature Communications; Vol. 11;
Art. No. 5644; PMCID PMC7648111; 10.1038/s41467-020-19539-6
- Dunham, Noah P. and Arnold, Frances H. (2020) Nature’s
Machinery, Repurposed: Expanding the Repertoire of Iron-Dependent
Oxygenases; ACS Catalysis; Vol. 10; No. 20; 12239-12255; PMCID
PMC7710332; 10.1021/acscatal.0c03606
- Bähr, Susanne; Brinkmann-Chen, Sabine; et el. (2020) Selective
Enzymatic Oxidation of Silanes to Silanols; Angewandte Chemie
International Edition; Vol. 59; No. 36; 15507-15511; PMCID PMC7511438;
10.1002/anie.202002861
- Wu, Zachary; Yang, Kevin K.; et el. (2020) Signal
Peptides Generated by Attention-Based Neural Networks; ACS Synthetic
Biology; Vol. 9; No. 8; 2154-2161; 10.1021/acssynbio.0c00219
- Wittmann, Bruce J.; Knight, Anders M.; et el. (2020) Diversity-Oriented
Enzymatic Synthesis of Cyclopropane Building Blocks; ACS Catalysis;
Vol. 10; No. 13; 7112-7116; PMCID PMC7709968; 10.1021/acscatal.0c01888
- Jia, Zhi-Jun; Gao, Shilong; et el. (2020) Enzymatic
Primary Amination of Benzylic and Allylic C(sp³)–H Bonds; Journal of
the American Chemical Society; Vol. 142; No. 23; 10279-10283; 10.1021/jacs.0c03428
- Zhou, Andrew Z.; Chen, Kai; et el. (2020) Enzymatic
Lactone-Carbene C‒H Insertion to Build Contiguous Chiral Centers;
ACS Catalysis; Vol. 10; No. 10; 5393-5398; 10.1021/acscatal.0c01349
- Chen, Kai and Arnold, Frances H. (2020) Engineering
cytochrome P450s for enantioselective cyclopropenation of internal
alkynes; Journal of the American Chemical Society; Vol. 142; No. 15;
6891-6895; 10.1021/jacs.0c01313
- Chen, Kai and Arnold, Frances H. (2020) Engineering
new catalytic activities in enzymes; Nature Catalysis; Vol. 3;
No. 3; 203-213; 10.1038/s41929-019-0385-5
- Watkins, Ella J.; Almhjell, Patrick J.; et el. (2020) Direct
enzymatic synthesis of a deep-blue fluorescent noncanonical amino acid
from azulene and serine; ChemBioChem; Vol. 21; No. 1-2; 80-83; PMCID
PMC6980211; 10.1002/cbic.201900497
- Goldberg, Nathaniel W.; Knight, Anders M.; et el. (2019) Nitrene
Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native
Small-Molecule Ligands; Journal of the American Chemical Society;
Vol. 141; No. 50; 19585-19588; PMCID PMC6924163; 10.1021/jacs.9b11608
- Dick, Markus; Sarai, Nicholas S.; et el. (2019) Tailoring
Tryptophan Synthase TrpB for Selective Quaternary Carbon Bond
Formation; Journal of the American Chemical Society; Vol. 141;
No. 50; 19817-19822; PMCID PMC6939453; 10.1021/jacs.9b09864
- Yang, Yang; Cho, Inha; et el. (2019) An
enzymatic platform for the asymmetric amination of primary, secondary
and tertiary C(sp³)–H bonds; Nature Chemistry; Vol. 11; No. 11;
987-993; PMCID PMC6998391; 10.1038/s41557-019-0343-5
- Bedbrook, Claire N.; Yang, Kevin K.; et el. (2019) Machine
learning-guided channelrhodopsin engineering enables minimally-invasive
optogenetics; Nature Methods; Vol. 16; No. 11; 1176-1184; PMCID
PMC6858556; 10.1038/s41592-019-0583-8
- Arnold, Frances H. (2019) Innovation
by Evolution: Bringing New Chemistry to Life (Nobel Lecture);
Angewandte Chemie International Edition; Vol. 58; No. 41; 14420-14426;
10.1002/anie.201907729
- Brandenberg, Oliver F.; Miller, David C.; et el. (2019) Engineering
Chemoselectivity in Hemoprotein-Catalyzed Indole Amidation; ACS
Catalysis; Vol. 9; No. 9; 8271-8275; PMCID PMC6959474; 10.1021/acscatal.9b02508
- Romney, David K.; Sarai, Nicholas S.; et el. (2019) Nitroalkanes
as Versatile Nucleophiles for Enzymatic Synthesis of Noncanonical Amino
Acids; ACS Catalysis; Vol. 9; No. 9; 8726-8730; PMCID PMC7709965; 10.1021/acscatal.9b02089
- Yang, Kevin K.; Wu, Zachary; et el. (2019) Machine-learning-guided
directed evolution for protein engineering; Nature Methods; Vol. 16;
No. 8; 687-694; 10.1038/s41592-019-0496-6
- Zhang, Juner; Huang, Xiongyi; et el. (2019) Enantiodivergent
α-Amino C–H Fluoroalkylation Catalyzed by Engineered Cytochrome
P450s; Journal of the American Chemical Society; Vol. 141; No. 25;
9798-9802; PMCID PMC6666315; 10.1021/jacs.9b04344
- Brandenberg, Oliver F.; Chen, Kai; et el. (2019) Directed
Evolution of a Cytochrome P450 Carbene Transferase for Selective
Functionalization of Cyclic Compounds; Journal of the American
Chemical Society; Vol. 141; No. 22; 8989-8995; 10.1021/jacs.9b02931
- Wu, Zachary; Kan, S. B. Jennifer; et el. (2019) Machine
learning-assisted directed protein evolution with combinatorial
libraries; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 116; No. 18; 8852-8858; PMCID PMC6500146;
10.1073/pnas.1901979116
- Zhang, Ruijie K.; Huang, Xiongyi; et el. (2019) Selective
C-H bond functionalization with engineered heme proteins: new tools to
generate complexity; Current Opinion in Chemical Biology; Vol. 49;
67-75; PMCID PMC6461521; 10.1016/j.cbpa.2018.10.004
- Cho, Inha; Prier, Christopher K.; et el. (2019) Enantioselective
Aminohydroxylation of Styrenyl Olefins Catalyzed by an Engineered
Hemoprotein; Angewandte Chemie International Edition; Vol. 58;
No. 10; 3138-3142; 10.1002/anie.201812968
- Chen, Kai; Huang, Xiongyi; et el. (2019) Engineered
Cytochrome c-Catalyzed Lactone-Carbene B–H Insertion; Synlett; Vol.
30; No. 4; 378-382; PMCID PMC6436545; 10.1055/s-0037-1611662
- Huang, Xiongyi; Garcia-Borràs, Marc; et el. (2019) A
Biocatalytic Platform for Synthesis of Chiral α-Trifluoromethylated
Organoborons; ACS Central Science; Vol. 5; No. 2; 270-276; PMCID
PMC6396380; 10.1021/acscentsci.8b00679
- Zhang, Ruijie K.; Chen, Kai; et el. (2019) Enzymatic
assembly of carbon–carbon bonds via iron-catalysed sp³ C–H
functionalization; Nature; Vol. 565; No. 7737; 67-72; PMCID
PMC6440214; 10.1038/s41586-018-0808-5
- Arnold, Frances H. and Strickland, Donna H. (2019) Nobel
Prizes 2018; Deutsche Lebensmittel-Rundschau; Vol. 115; No. 1;
40-41
- Almhjell, Patrick J.; Boville, Christina E.; et el. (2018) Engineering
enzymes for noncanonical amino acid synthesis; Chemical Society
Reviews; Vol. 47; No. 24; 8980-8997; PMCID PMC6434697; 10.1039/c8cs00665b
- Chen, Kai; Zhang, Shuo-Qing; et el. (2018) Alternate
heme ligation steers activity and selectivity in engineered cytochrome
P450-catalyzed carbene transfer reactions; Journal of the American
Chemical Society; Vol. 140; No. 48; 16402-16407; 10.1021/jacs.8b09613
- Boville, Christina E.; Scheele, Remkes A.; et el. (2018) Engineered
biosynthesis of β‐alkyl tryptophan analogs; Angewandte Chemie
International Edition; Vol. 57; No. 45; 14764-14768; 10.1002/anie.201807998
- Yang, Kevin K.; Wu, Zachary; et el. (2018) Learned
Protein Embeddings for Machine Learning; Bioinformatics; Vol. 34;
No. 15; 2642-2648; PMCID PMC6061698; 10.1093/bioinformatics/bty178
- Romney, David K.; Arnold, Frances H.; et el. (2018) Chemistry
Takes a Bath: Reactions in Aqueous Media; Journal of Organic
Chemistry; Vol. 83; No. 14; 7319-7322; 10.1021/acs.joc.8b01412
- Boville, Christina E.; Romney, David K.; et el. (2018) Improved
synthesis of 4-cyanotryptophan and other tryptophan analogs in aqueous
solvent using variants of TrpB from Thermotoga maritima; Journal of
Organic Chemistry; Vol. 83; No. 14; 7447-7452; PMCID PMC6054569; 10.1021/acs.joc.8b00517
- Lewis, Russell D.; Garcia-Borràs, Marc; et el. (2018) Catalytic
iron-carbene intermediate revealed in a cytochrome c carbene
transferase; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 115; No. 28; 7308-7313; PMCID PMC6048479;
10.1073/pnas.1807027115
- Buller, Andrew R.; van Roye, Paul; et el. (2018) Directed
evolution mimics allosteric activation by stepwise tuning of the
conformational ensemble; Journal of the American Chemical Society;
Vol. 140; No. 23; 7256-7266; PMCID PMC5999571; 10.1021/jacs.8b03490
- Arnold, Frances H. (2018) Directed
Evolution: Bringing New Chemistry to Life; Angewandte Chemie
International Edition; Vol. 57; No. 16; 4143-4148; PMCID PMC5901037; 10.1002/anie.201708408
- Chen, Kai; Huang, Xiongyi; et el. (2018) Enzymatic
construction of highly strained carbocycles; Science; Vol. 360;
No. 6384; 71-75; PMCID PMC6104391; 10.1126/science.aar4239
- Brandenberg, Oliver F.; Prier, Christopher K.; et el. (2018) Stereoselective
Enzymatic Synthesis of Heteroatom-Substituted Cyclopropanes; ACS
Catalysis; Vol. 8; No. 4; 2629-2634; 10.1021/acscatal.7b04423
- Knight, Anders M.; Kan, S. B. Jennifer; et el. (2018) Diverse
engineered heme proteins enable stereodivergent cyclopropanation of
unactivated alkenes; ACS Central Science; Vol. 4; No. 3; 372-377;
PMCID PMC5879470; 10.1021/acscentsci.7b00548
- Kan, S. B. Jennifer; Huang, Xiongyi; et el. (2017) Genetically
programmed chiral organoborane synthesis; Nature; Vol. 552;
No. 7683; 132-136; PMCID PMC5819735; 10.1038/nature24996
- Bedbrook, Claire N.; Yang, Kevin K.; et el. (2017) Machine
learning to design integral membrane channelrhodopsins for efficient
eukaryotic expression and plasma membrane localization; PLOS
Computational Biology; Vol. 13; No. 10; Art. No. e1005786; PMCID
PMC5695628; 10.1371/journal.pcbi.1005786
- Hammer, Stephan C.; Kubik, Grzegorz; et el. (2017) Anti-Markovnikov
alkene oxidation by metal-oxo–mediated enzyme catalysis; Science;
Vol. 358; No. 6360; 215-218; 10.1126/science.aao1482
- Hammer, Stephan C.; Knight, Anders M.; et el. (2017) Design
and Evolution of Enzymes for Non-natural Chemistry; Current Opinion
in Green and Sustainable Chemistry; Vol. 7; 23-30; 10.1016/j.cogsc.2017.06.002
- Brandenberg, Oliver F.; Fasan, Rudi; et el. (2017) Exploiting
and engineering hemoproteins for abiological carbene and nitrene
transfer reactions; Current Opinion in Biotechnology; Vol. 47;
102-111; PMCID PMC5617781; 10.1016/j.copbio.2017.06.005
- Romney, David K.; Murciano-Calles, Javier; et el. (2017) Unlocking
Reactivity of TrpB: A General Biocatalytic Platform for Synthesis of
Tryptophan Analogues; Journal of the American Chemical Society; Vol.
139; No. 31; 10769-10776; PMCID PMC5589443; 10.1021/jacs.7b05007
- Romney, David K.; Murciano-Calles, Javier; et el. (2017) Unlocking
reactivity of TrpB: a general biocatalytic platform for synthesis of
tryptophan analogs; Journal of the American Chemical Society; Vol.
131; No. 39; 10769-10776; PMCID PMC5589443; 10.1021/jacs.7b05007
- Loskot, Steven A.; Romney, David K.; et el. (2017) Enantioselective
Total Synthesis of Nigelladine A via Late-Stage C–H Oxidation Enabled by
an Engineered P450 Enzyme; Journal of the American Chemical Society;
Vol. 139; No. 30; 10196-10199; PMCID PMC5679227; 10.1021/jacs.7b05196
- Prier, Christopher K.; Zhang, Ruijie K.; et el. (2017) Enantioselective,
intermolecular benzylic C–H amination catalysed by an engineered
iron-haem enzyme; Nature Chemistry; Vol. 9; No. 7; 629-634; PMCID
PMC5555633; 10.1038/nchem.2783
- Bedbrook, Claire N.; Rice, Austin J.; et el. (2017) Structure-guided
SCHEMA recombination generates diverse chimeric channelrhodopsins;
Proceedings of the National Academy of Sciences of the United States of
America; Vol. 114; No. 13; E2624-E2633; PMCID PMC5380088; 10.1073/pnas.1700269114
- Herwig, Lukas; Rice, Austin J.; et el. (2017) Directed
Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a
Synthetic Chromophore; Cell Chemical Biology; Vol. 24; No. 3;
415-425; PMCID PMC5357175; 10.1016/j.chembiol.2017.02.008
- Cahn, Jackson K. B.; Werlang, Caroline A.; et el. (2017) A
General Tool for Engineering the NAD/NADP Cofactor Preference of
Oxidoreductases; ACS Synthetic Biology; Vol. 6; No. 2; 326-333;
PMCID PMC8611728; 10.1021/acssynbio.6b00188
- Buller, Andrew R.; van Roye, Paul; et el. (2016) Tryptophan
synthase uses an atypical mechanism to achieve substrate
specificity; Biochemistry; Vol. 55; No. 51; 7043-7046; PMCID
PMC5207025; 10.1021/acs.biochem.6b01127
- Kan, S. B. Jennifer; Lewis, Russell D.; et el. (2016) Directed
evolution of cytochrome c for carbon–silicon bond formation: Bringing
silicon to life; Science; Vol. 354; No. 6315; 1048-1051; PMCID
PMC5243118; 10.1126/science.aah6219
- Hernandez, Kari E.; Renata, Hans; et el. (2016) Highly
Stereoselective Biocatalytic Synthesis of Key Cyclopropane Intermediate
to Ticagrelor; ACS Catalysis; Vol. 6; No. 11; 7810-7813; PMCID
PMC5340201; 10.1021/acscatal.6b02550
- Renata, Hans; Lewis, Russell D.; et el. (2016) Identification
of Mechanism-Based Inactivation in P450-Catalyzed Cyclopropanation
Facilitates Engineering of Improved Enzymes; Journal of the American
Chemical Society; Vol. 138; No. 38; 12527-12533; PMCID PMC5042878; 10.1021/jacs.6b06823
- Murciano-Calles, Javier; Romney, David K.; et el. (2016) A
Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the
Transfer of Mutations that Mimic Allosteric Activation; Angewandte
Chemie International Edition; Vol. 55; No. 38; 11577-11581; PMCID
PMC5014574; 10.1002/anie.201606242
- Moraïs, Sarah; Stern, Johanna; et el. (2016) Enhancement
of cellulosome-mediated deconstruction of cellulose by improving enzyme
thermostability; Biotechnology for Biofuels; Vol. 9; Art. No. 164;
PMCID PMC4973527; 10.1186/s13068-016-0577-z
- Herger, Michael; van Roye, Paul; et el. (2016) Synthesis
of β-Branched Tryptophan Analogues Using an Engineered Subunit of
Tryptophan Synthase; Journal of the American Chemical Society; Vol.
138; No. 27; 8388-8391; PMCID PMC4948191; 10.1021/jacs.6b04836
- Cahn, Jackson K. B.; Brinkmann-Chen, Sabine; et el. (2016) Artificial
domain duplication replicates evolutionary history of ketol-acid
reductoisomerases; Protein Science; Vol. 25; No. 7; 1241-1248; PMCID
PMC4918412; 10.1002/pro.2852
- Dodani, Sheel C.; Kiss, Gert; et el. (2016) Discovery
of a regioselectivity switch in nitrating P450s guided by molecular
dynamics simulations and Markov models; Nature Chemistry; Vol. 8;
No. 5; 419-425; PMCID PMC4843824; 10.1038/NCHEM.2474
- Prier, Christopher K.; Hyster, Todd K.; et el. (2016) Asymmetric
Enzymatic Synthesis of Allylic Amines: A Sigmatropic Rearrangement
Strategy; Angewandte Chemie International Edition; Vol. 55; No. 15;
4711-4715; PMCID PMC4818679; 10.1002/anie.201601056
- Chang, Chia-Jung; Lee, Cheng-Chung; et el. (2016) Exploring
the Mechanism Responsible for Cellulase Thermostability by
Structure-Guided Recombination; PLoS ONE; Vol. 11; No. 3; Art.
No. e0147485; PMCID PMC4795783; 10.1371/journal.pone.0147485
- Rice, Austin J.; Bedbrook, Claire N.; et el. (2016) Rhodopsin
Engineering through Structure-Guided Recombination; Biophysical
Journal; Vol. 110; No. 3; 170A; 10.1016/j.bpj.2015.11.947
- Cahn, J. K. B.; Baumschlager, A.; et el. (2016) Mutations
in adenine-binding pockets enhance catalytic properties of
NAD(P)H-dependent enzymes; Protein Engineering Design and Selection;
Vol. 29; No. 1; 31-38; PMCID PMC4678007; 10.1093/protein/gzv057
- Buller, Andrew R.; Brinkmann-Chen, Sabine; et el. (2015) Directed
evolution of the tryptophan synthase β-subunit for stand-alone function
recapitulates allosteric activation; Proceedings of the National
Academy of Sciences of the United States of America; Vol. 112; No. 47;
14599-14604; PMCID PMC4664345; 10.1073/pnas.1516401112
- Prier, Christopher K. and Arnold, Frances H. (2015) Chemomimetic
Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent
Enzymes To Create New Catalysts; Journal of the American Chemical
Society; Vol. 137; No. 44; 13992-14006; 10.1021/jacs.5b09348
- McIntosh, John A.; Heel, Thomas; et el. (2015) Structural
Adaptability Facilitates Histidine Heme Ligation in a Cytochrome
P450; Journal of the American Chemical Society; Vol. 137; No. 43;
13861-13865; PMCID PMC4635421; 10.1021/jacs.5b07107
- Arnold, Frances H. (2015) The
nature of chemical innovation: new enzymes by evolution; Quarterly
Reviews of Biophysics; Vol. 48; No. 4; 404-410; 10.1017/S003358351500013X
- Bedbrook, Claire N.; Kato, Mihoko; et el. (2015) Genetically
Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized
Proteins In Vivo; Chemistry and Biology; Vol. 22; No. 8; 1108-1121;
PMCID PMC4546540; 10.1016/j.chembiol.2015.06.020
- McIsaac, R. Scott; Bedbrook, Claire N.; et el. (2015) Recent
advances in engineering microbial rhodopsins for optogenetics;
Current Opinion in Structural Biology; Vol. 33; 8-15; PMCID PMC4641784;
10.1016/j.sbi.2015.05.001
- Cahn, Jackson K. B.; Brinkmann-Chen, Sabine; et el. (2015) Cofactor
specificity motifs and the induced fit mechanism in class I ketol-acid
reductoisomerases; Biochemical Journal; Vol. 468; No. 3; 475-484;
PMCID PMC4550535; 10.1042/BJ20150183
- Farwell, Christopher C.; Zhang, Ruijie K.; et el. (2015) Enantioselective
Enzyme-Catalyzed Aziridination Enabled by Active-Site Evolution of a
Cytochrome P450; ACS Central Science; Vol. 1; No. 2; 89-93; PMCID
PMC4571169; 10.1021/acscentsci.5b00056
- Renata, Hans; Wang, Z. Jane; et el. (2015) Expanding
the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided
Directed Evolution; Angewandte Chemie International Edition; Vol.
54; No. 11; 3351-3367; PMCID PMC4404643; 10.1002/anie.201409470
- Engqvist, Martin K. M.; McIsaac, R. Scott; et el. (2015) Directed
Evolution of Gloeobacter violaceus Rhodopsin Spectral Properties;
Journal of Molecular Biology; Vol. 427; No. 1; 205-220; 10.1016/j.jmb.2014.06.015
- Hyster, Todd K. and Arnold, Frances H. (2015) P450_(BM3)-Axial
Mutations: A Gateway to Non-Natural Reactivity; Israel Journal of
Chemistry; Vol. 55; No. 1; 14-20; 10.1002/ijch.201400080
- Trudeau, Devin L.; Lee, Toni M.; et el. (2014) Engineered
thermostable fungal cellulases exhibit efficient synergistic cellulose
hydrolysis at elevated temperatures; Biotechnology and
Bioengineering; Vol. 111; No. 12; 2390-2397; 10.1002/bit.25308
- Heel, Thomas; McIntosh, John A.; et el. (2014) Non-natural
Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other
Hemoproteins; ChemBioChem; Vol. 15; No. 17; 2556-2562; PMCID
PMC4287214; 10.1002/cbic.201402286
- Hyster, Todd K.; Farwell, Christopher C.; et el. (2014) Enzyme-Controlled
Nitrogen-Atom Transfer Enables Regiodivergent C−H Amination; Journal
of the American Chemical Society; Vol. 136; No. 44; 15505-15508; PMCID
PMC4227740; 10.1021/ja509308v
- Brinkmann-Chen, S.; Cahn, J. K. B.; et el. (2014) Uncovering
rare NADH-preferring ketol-acid reductoisomerases; Metabolic
Engineering; Vol. 26; 17-22; 10.1016/j.ymben.2014.08.003
- Dodani, Sheel C.; Cahn, Jackson K. B.; et el. (2014) Structural,
Functional, and Spectroscopic Characterization of the Substrate Scope of
the Novel Nitrating Cytochrome P450 TxtE; ChemBioChem; Vol. 15;
No. 15; 2259-2267; PMCID PMC4260628; 10.1002/cbic.201402241
- Renata, Hans; Wang, Z. Jane; et el. (2014) P450-catalyzed
asymmetric cyclopropanation of electron-deficient olefins under aerobic
conditions; Catalysis Science and Technology; Vol. 4; No. 10;
3640-3643; PMCID PMC4159215; 10.1039/c4cy00633j
- Flytzanis, Nicholas C.; Bedbrook, Claire N.; et el. (2014) Archaerhodopsin
variants with enhanced voltage-sensitive fluorescence in mammalian and
Caenorhabditis elegans neurons; Nature Communications; Vol. 5;
No. 9; Art. No. 4894; PMCID PMC4166526; 10.1038/ncomms5894
- McIsaac, R. Scott; Engqvist, Martin K. M.; et el. (2014) Directed
evolution of a far-red fluorescent rhodopsin; Proceedings of the
National Academy of Sciences of the United States of America; Vol. 111;
No. 36; 13034-13039; PMCID PMC4246972; 10.1073/pnas.1413987111
- Sun, Fei; Zhang, Wen-Bin; et el. (2014) Synthesis
of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher
chemistry; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 111; No. 31; 11269-11274; PMCID
PMC4128157; 10.1073/pnas.1401291111
- Lin, Paul P.; Rabe, Kersten S.; et el. (2014) Isobutanol
production at elevated temperatures in thermophilic Geobacillus
thermoglucosidasius; Metabolic Engineering; Vol. 24; 1-8; 10.1016/j.ymben.2014.03.006
- Wang, Jane Z.; Renata, Hans; et el. (2014) Improved
Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables
the Enantioselective Formal Synthesis of Levomilnacipran; Angewandte
Chemie International Edition; Vol. 53; No. 26; 6810-6813; PMCID
PMC4120663; 10.1002/anie.201402809
- Farwell, Christopher C.; McIntosh, John A.; et el. (2014) Enantioselective
Imidation of Sulfides via Enzyme-Catalyzed Intermolecular Nitrogen-Atom
Transfer; Journal of the American Chemical Society; Vol. 136;
No. 24; 8766-8771; PMCID PMC4154708; 10.1021/ja503593n
- Arnold, Frances H. and Meyerowitz, Joseph T. (2014) Synthetic
biology: Engineering explored; Nature; Vol. 509; No. 7499; 166-167;
10.1038/509166a
- McIntosh, John A.; Farwell, Christopher C.; et el. (2014) Expanding
P450 catalytic reaction space through evolution and engineering;
Current Opinion in Chemical Biology; Vol. 19; 126-134; PMCID PMC4008644;
10.1016/j.cbpa.2014.02.001
- Ener, M. E.; Coelho, P. S.; et el. (2014) Photo-initiated
and electron transfer reactivity of ruthenium-labeled cytochrome
P450; Journal of Biological Inorganic Chemistry; Vol. 19; No. S1;
S219; 10.1007/s00775-014-1095-8
- Smith, Matthew A.; Bedbrook, Claire N.; et el. (2013) Hypocrea
jecorina Cellobiohydrolase I Stabilizing Mutations Identified Using
Noncontiguous Recombination; ACS Synthetic Biology; Vol. 2; No. 12;
690-696; 10.1021/sb400010m
- Trudeau, Devin L.; Smith, Matthew A.; et el. (2013) Innovation
by homologous recombination; Current Opinion in Chemical Biology;
Vol. 17; No. 6; 902-909; 10.1016/j.cbpa.2013.10.007
- Wang, Z. Jane; Peck, Nicole E.; et el. (2013) Cytochrome
P450-catalyzed insertion of carbenoids into N–H bonds; Chemical
Science; Vol. 5; No. 2; 598-601; PMCID PMC3906682; 10.1039/c3sc52535j
- Zhang, Wen-Bin; Sun, Fei; et el. (2013) Controlling
Macromolecular Topology with Genetically Encoded SpyTag-SpyCatcher
Chemistry; Journal of the American Chemical Society; Vol. 135;
No. 37; 13988-13997; 10.1021/ja4076452
- McIntosh, John A.; Coelho, Pedro S.; et el. (2013) Enantioselective
Intramolecular C-H Amination Catalyzed by Engineered Cytochrome P450
Enzymes In Vitro and In Vivo; Angewandte Chemie International
Edition; Vol. 52; No. 35; 9309-9312; PMCID PMC3988694; 10.1002/anie.201304401
- Coelho, Pedro S.; Wang, Z. Jane; et el. (2013) A
serine-substituted P450 catalyzes highly efficient carbene transfer to
olefins in vivo; Nature Chemical Biology; Vol. 9; No. 8; 485-487;
PMCID PMC3720782; 10.1038/nchembio.1278
- Wu, Indira; Heel, Thomas; et el. (2013) Role
of cysteine residues in thermal inactivation of fungal Cel6A
cellobiohydrolases; Biochimica et Biophysica Acta - Proteins and
Proteomics; Vol. 1834; No. 8; 1539-1544; 10.1016/j.bbapap.2013.05.003
- Brinkmann-Chen, Sabine; Flock, Tilman; et el. (2013) General
approach to reversing ketol-acid reductoisomerase cofactor dependence
from NADPH to NADH; Proceedings of the National Academy of Sciences
of the United States of America; Vol. 110; No. 27; 10946-10951; PMCID
PMC3704004; 10.1073/pnas.1306073110
- Wu, Indira and Arnold, Frances H. (2013) Engineered
thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze
cellulose efficiently at elevated temperatures; Biotechnology and
Bioengineering; Vol. 110; No. 7; 1874-1883; 10.1002/bit.24864
- Lauchli, Ryan; Rabe, Kersten S.; et el. (2013) High-Throughput
Screening for Terpene-Synthase-Cyclization Activity and Directed
Evolution of a Terpene Synthase; Angewandte Chemie International
Edition; Vol. 52; No. 21; 5571-5574; PMCID PMC3828198; 10.1002/anie.201301362
- Smith, Matthew A.; Romero, Philip A.; et el. (2013) Chimeragenesis
of distantly-related proteins by noncontiguous recombination;
Protein Science; Vol. 22; No. 2; 231-238; PMCID PMC3588919; 10.1002/pro.2202
- Coelho, Pedro S.; Brustad, Eric M.; et el. (2013) Olefin
Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome
P450 Enzymes; Science; Vol. 339; No. 6117; 307-310; 10.1126/science.1231434
- Romero, Philip A.; Krause, Andreas; et el. (2013) Navigating
the protein fitness landscape with Gaussian processes; Proceedings
of the National Academy of Sciences of the United States of America;
Vol. 110; No. 3; E193-E201; PMCID PMC3549130; 10.1073/pnas.1215251110
- Liu, Xiang; Bastian, Sabine; et el. (2012) Structure-guided
engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for
improved conversion of isobutyraldehyde to isobutanol; Journal of
Biotechnology; Vol. 164; No. 2; 188-195; PMCID PMC3542407; 10.1016/j.jbiotec.2012.08.008
- Smith, Matthew A.; Rentmeister, Andrea; et el. (2012) A
diverse set of family 48 bacterial glycoside hydrolase cellulases
created by structure-guided recombination; FEBS Journal; Vol. 279;
No. 24; 4453-4465; 10.1111/febs.12032
- Komor, Russell S.; Romero, Philip A.; et el. (2012) Highly
thermostable fungal cellobiohydrolase I (Cel7A) engineered using
predictive methods; Protein Engineering, Design and Selection; Vol.
25; No. 12; 827-833; 10.1093/protein/gzs058
- Romero, Philip A. and Arnold, Frances H. (2012) Random
Field Model Reveals Structure of the Protein Recombinational
Landscape; PLoS Computational Biology; Vol. 8; No. 10; Art.
No. e1002713; PMCID PMC3464211; 10.1371/journal.pcbi.1002713
- Brustad, Eric M.; Lelyveld, Victor S.; et el. (2012) Structure-Guided
Directed Evolution of Highly Selective P450-Based Magnetic Resonance
Imaging Sensors for Dopamine and Serotonin; Journal of Molecular
Biology; Vol. 422; No. 2; 245-262; PMCID PMC3418479; 10.1016/j.jmb.2012.05.029
- Lee, Toni; Farrow, Mary; et el. (2012) Computational
Thermostable Cellulase Engineering for Enhanced Biofuel Production;
Protein Science; Vol. 21; No. S1; 71-72
- Snow, Christopher D.; Brustad, Eric; et el. (2012) High
Resolution Models from Low Resolution Data: Recombination of Fragments
for Crystallographic Structure Determination; Protein Science; Vol.
21; No. S1; 91-92
- Lelyveld, Victor; Brustad, Eric; et el. (2012) Metalloprotein-based
contrast sensors for neurochemical detection by MRI; Protein
Science; Vol. 21; No. S1; 184
- Romero, Philip A.; Stone, Everett; et el. (2012) SCHEMA-Designed
Variants of Human Arginase I and II Reveal Sequence Elements Important
to Stability and Catalysis; ACS Synthetic Biology; Vol. 1; No. 6;
221-228; PMCID PMC3378063; 10.1021/sb300014t
- Chen, Mike M. Y.; Snow, Christopher D.; et el. (2012) Comparison
of random mutagenesis and semi-rational designed libraries for improved
cytochrome P450 BM3-catalyzed hydroxylation of small alkanes;
Protein Engineering, Design and Selection; Vol. 25; No. 4; 171-178; 10.1093/protein/gzs004
- Chen, Mike M. Y.; Coelho, Pedro S.; et el. (2012) Utilizing
Terminal Oxidants to Achieve P450-Catalyzed Oxidation of Methane;
Advanced Synthesis and Catalysis; Vol. 354; No. 6; 964-968; 10.1002/adsc.201100833
- Jung, Sang Taek; Lauchli, Ryan; et el. (2011) Cytochrome
P450: taming a wild type enzyme; Current Opinion in Biotechnology;
Vol. 22; No. 6; 809-817; PMCID PMC3118264; 10.1016/j.copbio.2011.02.008
- Lee, Toni M.; Farrow, Mary F.; et el. (2011) A
structural study of Hypocrea jecorina Cel5A; Protein Science; Vol.
20; No. 11; 1935-1940; PMCID PMC3267957; 10.1002/pro.730
- Farrow, Mary F. and Arnold, Frances H. (2011) High
Throughput Screening of Fungal Endoglucanase Activity in Escherichia
coli; Journal of Visualized Experiments; Vol. 54; Art. No. e2942;
PMCID PMC3217630; 10.3791/2942
- Rentmeister, Andrea; Brown, Tristan R.; et el. (2011) Engineered
Bacterial Mimics of Human Drug Metabolizing Enzyme CYP2C9;
ChemCatChem; Vol. 3; No. 6; 1065-1071; 10.1002/cctc.201000452
- Bastian, Sabine; Liu, Xiang; et el. (2011) Engineered
ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic
2-methylpropan-1-ol production at theoretical yield in Escherichia
coli; Metabolic Engineering; Vol. 13; No. 3; 345-352; 10.1016/j.ymben.2011.02.004
- Brustad, Eric M. and Arnold, Frances H. (2011) Optimizing
non-natural protein function with directed evolution; Current
Opinion in Chemical Biology; Vol. 15; No. 2; 201-210; PMCID PMC3080047;
10.1016/j.cbpa.2010.11.020
- Fasan, Rudi; Crook, Nathan C.; et el. (2011) Improved
Product-Per-Glucose Yields in P450-Dependent Propane Biotransformations
Using Engineered Escherichia coli; Biotechnology and Bioengineering;
Vol. 108; No. 3; 500-510; 10.1002/bit.22984
- Brenner, Katie and Arnold, Frances H. (2011) Self-Organization,
Layered Structure, and Aggregation Enhance Persistence of a Synthetic
Biofilm Consortium; PLoS ONE; Vol. 6; No. 2; Art. No. e16791; PMCID
PMC3036657; 10.1371/journal.pone.0016791
- Lelyveld, Victor S.; Brustad, Eric; et el. (2011) Metal-Substituted
Protein MRI Contrast Agents Engineered for Enhanced Relaxivity and
Ligand Sensitivity; Journal of the American Chemical Society; Vol.
133; No. 4; 649-651; PMCID PMC3154012; 10.1021/ja107936d
- Mingardon, Florence; Bagert, John D.; et el. (2011) Comparison
of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria;
Applied and Environmental Microbiology; Vol. 77; No. 4; 1436-1442; PMCID
PMC3067227; 10.1128/AEM.01802-10
- Lewis, Jared C.; Coelho, Pedro S.; et el. (2011) Enzymatic
functionalization of carbon-hydrogen bonds; Chemical Society
Reviews; Vol. 40; No. 4; 2003-2021; PMCID PMC3064445; 10.1039/c0cs00067a
- Lewis, Jared C.; Mantovani, Simone M.; et el. (2010) Combinatorial
Alanine Substitution Enables Rapid Optimization of Cytochrome P450_(BM3)
for Selective Hydroxylation of Large Substrates; ChemBioChem; Vol.
11; No. 18; 2502-2505; PMCID PMC4447097; 10.1002/cbic.201000565
- Farrow, Mary F. and Arnold, Frances H. (2010) Combinatorial
Recombination of Gene Fragments to Construct a Library of Chimeras;
Current Protocols in Protein Science; Vol. 62; No. 1; 26.2.1-26.2.20; 10.1002/0471140864.ps2602s61
- Heinzelman, Pete; Komor, Russell; et el. (2010) Efficient
screening of fungal cellobiohydrolase class I enzymes for
thermostabilizing sequence blocks by SCHEMA structure-guided
recombination; Protein Engineering, Design and Selection; Vol. 23;
No. 11; 871-880; 10.1093/protein/gzq063
- Acar, Murat; Pando, Bernardo F.; et el. (2010) A
General Mechanism for Network-Dosage Compensation in Gene Circuits;
Science; Vol. 329; No. 5999; 1656-1660; PMCID PMC3138731; 10.1126/science.1190544
- Jäckel, Christian; Bloom, Jesse D.; et el. (2010) Consensus
Protein Design without Phylogenetic Bias; Journal of Molecular
Biology; Vol. 399; No. 4; 541-546; PMCID PMC2904866; 10.1016/j.jmb.2010.04.039
- Shapiro, Mikhail G.; Westmeyer, Gil G.; et el. (2010) Directed
evolution of a magnetic resonance imaging contrast agent for noninvasive
imaging of dopamine; Nature Biotechnology; Vol. 28; No. 3; 264-270;
PMCID PMC3073400; 10.1038/nbt.1609
- Romero, Philip A. and Arnold, Frances H. (2009) Exploring
protein fitness landscapes by directed evolution; Nature Reviews.
Molecular Cell Biology; Vol. 10; No. 12; 866-876; PMCID PMC2997618; 10.1038/nrm2805
- Sawayama, Andrew M.; Chen, Michael M. Y.; et el. (2009) A
Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites and
Diversify Lead Compounds; Chemistry: a European Journal; Vol. 15;
No. 43; 11723-11729; PMCID PMC3118466; 10.1002/chem.200900643
- Sawayama, Andrew M.; Chen, Michael M. Y.; et el. (2009) A
Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites and
Diversify Lead Compounds; Chemistry: a European Journal; Vol. 15;
No. 43; 11723-11729; 10.1002/chem.200900643
- Lewis, Jared C.; Bastian, Sabine; et el. (2009) Chemoenzymatic
elaboration of monosaccharides using engineered cytochrome P450_(BM3)
demethylases; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 106; No. 39; 16550-16555; PMCID
PMC2757845; 10.1073/pnas.0908954106
- Heinzelman, Pete; Snow, Christopher D.; et el. (2009) SCHEMA
Recombination of a Fungal Cellulase Uncovers a Single Mutation That
Contributes Markedly to Stability; Journal of Biological Chemistry;
Vol. 284; No. 39; 26229-26233; PMCID PMC2785310; 10.1074/jbc.C109.034058
- Dougherty, Michael J. and Arnold, Frances H. (2009) Directed
evolution: new parts and optimized function; Current Opinion in
Biotechnology; Vol. 20; No. 4; 486-491; PMCID PMC2775421; 10.1016/j.copbio.2009.08.005
- Bloom, Jesse D. and Arnold, Frances H. (2009) In
the light of directed evolution: Pathways of adaptive protein
evolution; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 106; No. Supple; 9995-10000; PMCID
PMC2702793; 10.1073/pnas.0901522106
- Lewis, Jared C. and Arnold, Frances H. (2009) Catalysts
on Demand: Selective Oxidations by Laboratory-Evolved Cytochrome P450
BM3; Chimia; Vol. 63; No. 6; 309-312; 10.2533/chimia.2009.309
- Heinzelman, Pete; Snow, Christopher D.; et el. (2009) A
family of thermostable fungal cellulases created by structure-guided
recombination; Proceedings of the National Academy of Sciences of
the United States of America; Vol. 106; No. 14; 5610-5615; PMCID
PMC2667002; 10.1073/pnas.0901417106
- Tracewell, Cara A. and Arnold, Frances H. (2009) Directed
enzyme evolution: climbing fitness peaks one amino acid at a time;
Current Opinion in Chemical Biology; Vol. 13; No. 1; 3-9; PMCID
PMC2703427; 10.1016/j.cbpa.2009.01.017
- Rentmeister, Andrea; Arnold, Frances H.; et el. (2009) Chemo-enzymatic
fluorination of unactivated organic compounds; Nature Chemical
Biology; Vol. 5; No. 1; 26-28; PMCID PMC2713661; 10.1038/nchembio.128
- Koch, Daniel J.; Chen, Mike M.; et el. (2009) In Vivo
Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and
CYP153A6; Applied and Environmental Microbiology; Vol. 75; No. 2;
337-344; PMCID PMC2620723; 10.1128/AEM.01758-08
- Brenner, Katie; You, Lingchong; et el. (2009) Response to
Goldman and Brown: Making sense of microbial consortia using ecology and
evolution; Trends in Biotechnology; Vol. 27; No. 1; 4; 10.1016/j.tibtech.2008.10.004
- Fasan, Rudi; Meharenna, Yergalem T.; et el. (2008) Evolutionary
History of a Specialized P450 Propane Monooxygenase; Journal of
Molecular Biology; Vol. 383; No. 5; 1069-1080; PMCID PMC2637765; 10.1016/j.jmb.2008.06.060
- Brenner, Katie; You, Lingchong; et el. (2008) Engineering
microbial consortia: a new frontier in synthetic biology; Trends in
Biotechnology; Vol. 26; No. 9; 483-489; 10.1016/j.tibtech.2008.05.004
- Balagaddé, Frederick K.; Song, Hao; et el. (2008) A synthetic
Escherichia coli predator–prey ecosystem; Molecular Systems Biology;
Vol. 4; Art. No. 187; PMCID PMC2387235; 10.1038/msb.2008.24
- Haseltine, Eric L. and Arnold, Frances H. (2008) Implications
of Rewiring Bacterial Quorum Sensing; Applied and Environmental
Microbiology; Vol. 74; No. 2; 437-445; PMCID PMC2223271; 10.1128/AEM.01688-07
- Fasan, Rudi; Chen, Mike M.; et el. (2007) Engineered
Alkane‐Hydroxylating Cytochrome P450_(BM3) Exhibiting Nativelike
Catalytic Properties; Angewandte Chemie International Edition; Vol.
46; No. 44; 8414-8418; 10.1002/anie.200702616
- Brenner, Katie; Karig, David K.; et el. (2007) Engineered
bidirectional communication mediates a consensus in a microbial biofilm
consortium; Proceedings of the National Academy of Sciences of the
United States of America; Vol. 104; No. 44; 17300-17304; PMCID
PMC2077251; 10.1073/pnas.0704256104
- Li, Yougen; Drummond, D. Allan; et el. (2007) A
diverse family of thermostable cytochrome P450s created by recombination
of stabilizing fragments; Nature Biotechnology; Vol. 25; No. 9;
1051-1056; 10.1038/nbt1333
- Hawkins, Andrew C.; Arnold, Frances H.; et el. (2007) Directed
evolution of Vibrio fischeri LuxR for improved response to
butanoyl-homoserine lactone; Applied and Environmental Microbiology;
Vol. 73; No. 18; 5775-5781; PMCID PMC2074898; 10.1128/AEM.00060-07
- Carbone, Martina N. and Arnold, Frances H. (2007) Engineering
by homologous recombination: exploring sequence and function within a
conserved fold; Current Opinion in Structural Biology; Vol. 17;
No. 4; 454-459; 10.1016/j.sbi.2007.08.005
- Bloom, Jesse D.; Lu, Zhongyi; et el. (2007) Evolution
favors protein mutational robustness in sufficiently large
populations; BMC Biology; Vol. 5; No. 29; PMCID PMC1995189; 10.1186/1741-7007-5-29
- Bloom, Jesse D.; Romero, Philip A.; et el. (2007) Neutral
genetic drift can alter promiscuous protein functions, potentially
aiding functional evolution; Biology Direct; Vol. 2; Art. No. 17;
PMCID PMC1914045; 10.1186/1745-6150-2-17
- Haseltine, Eric L. and Arnold, Frances H. (2007) Synthetic
Gene Circuits: Design with Directed Evolution; Annual Review of
Biophysics and Biomolecular Structure; Vol. 36; 1-19; 10.1146/annurev.biophys.36.040306.132600
- Landwehr, Marco; Carbone, Martina; et el. (2007) Diversification
of Catalytic Function in a Synthetic Family of Chimeric Cytochrome
P450s; Chemistry and Biology; Vol. 14; No. 3; 269-278; PMCID
PMC1991292; 10.1016/j.chembiol.2007.01.009
- Bloom, Jesse D.; Arnold, Frances H.; et el. (2007) Breaking
proteins with mutations: threads and thresholds in evolution;
Molecular Systems Biology; Vol. 3; No. 1; Art. No. 76; PMCID PMC1800353;
10.1038/msb4100119
- Meyer, Michelle M.; Hochrein, Lisa; et el. (2006) Structure-guided
SCHEMA recombination of distantly related β-lactamase; Protein
Engineering, Design and Selection; Vol. 19; No. 12; 563-570; 10.1093/protein/gzl045
- Bloom, Jesse D.; Drummond, D. Allan; et el. (2006) Structural
Determinants of the Rate of Protein Evolution in Yeast; Molecular
Biology and Evolution; Vol. 23; No. 9; 1751-1761; 10.1093/molbev/msl040
- Collins, Cynthia H.; Leadbetter, Jared R.; et el. (2006) Dual
selection enhances the signaling specificity of a variant of the
quorum-sensing transcriptional activator LuxR; Nature Biotechnology;
Vol. 24; No. 6; 708-712; 10.1038/nbt1209
- Landwehr, Marco; Hochrein, Lisa; et el. (2006) Enantioselective
α-Hydroxylation of 2-Arylacetic Acid Derivatives and Buspirone Catalyzed
by Engineered Cytochrome P450 BM-3; Journal of the American Chemical
Society; Vol. 128; No. 18; 6058-6059; PMCID PMC2551755; 10.1021/ja061261x
- Otey, Christopher R.; Landwehr, Marco; et el. (2006) Structure-Guided
Recombination Creates an Artificial Family of Cytochromes P450; PLoS
Biology; Vol. 4; No. 5; e112; PMCID PMC1431580; 10.1371/journal.pbio.0040112
- Bloom, Jesse D.; Labthavikul, Sy T.; et el. (2006) Protein
stability promotes evolvability; Proceedings of the National Academy
of Sciences of the United States of America; Vol. 103; No. 15;
5869-5874; PMCID PMC1458665; 10.1073/pnas.0510098103
- Arnold, Frances H. (2006) Fancy
footwork in the sequence space shuffle; Nature Biotechnology; Vol.
24; No. 3; 328-330; 10.1038/nbt0306-328
- Udit, Andrew K.; Hill, Michael G.; et el. (2006) Pyrene-Wired
Heme Domain Cytochrome P450 BM3 Electrodes; ECS Meeting Abstracts;
Vol. 501; No. 1; Abstract 9
- Otey, Christopher R.; Bandara, Geethani; et el. (2006) Preparation
of human metabolites of propranolol using laboratory‐evolved bacterial
cytochromes P450; Biotechnology and Bioengineering; Vol. 93; No. 3;
494-499; 10.1002/bit.20744
- Tobias, Alexander V. and Arnold, Frances H. (2006) Biosynthesis
of novel carotenoid families based on unnatural carbon backbones: A
model for diversification of natural product pathways; Biochimica et
Biophysica Acta - Molecular and Cell Biology of Lipids; Vol. 1761;
No. 2; 235-46; 10.1016/j.bbalip.2006.01.003
- Kubo, Takafumi; Peters, Matthew W.; et el. (2006) Enantioselective
Epoxidation of Terminal Alkenes to (R)- and (S)-Epoxides by Engineered
Cytochromes P450 BM-3; Chemistry: a European Journal; Vol. 12;
No. 4; 1216-1220; 10.1002/chem.200500584
- Meinhold, Peter; Peters, Matthew W.; et el. (2005) Direct
Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3;
ChemBioChem; Vol. 6; No. 10; 1765-1768; 10.1002/cbic.200500261
- Drummond, D. Allan; Bloom, Jesse D.; et el. (2005) Why highly
expressed proteins evolve slowly; Proceedings of the National
Academy of Sciences of the United States of America; Vol. 102; No. 40;
14338-14343; PMCID PMC1242296; 10.1073/pnas.0504070102
- Yokobayashi, Yohei and Arnold, Frances H. (2005) A
Dual Selection Module for Directed Evolution of Genetic Circuits;
Natural Computing; Vol. 4; No. 3; 245-254; 10.1007/s11047-004-7442-x
- Bloom, Jesse D.; Meyer, Michelle M.; et el. (2005) Evolving
strategies for enzyme engineering; Current Opinion in Structural
Biology; Vol. 15; No. 4; 447-452; 10.1016/j.sbi.2005.06.004
- Drummond, D. Allan; Iverson, Brent L.; et el. (2005) Why
High-error-rate Random Mutagenesis Libraries are Enriched in Functional
and Improved Proteins; Journal of Molecular Biology; Vol. 350;
No. 4; 806-816; 10.1016/j.jmb.2005.05.023
- Balagaddé, Frederick K.; You, Lingchong; et el. (2005) Long-Term
Monitoring of Bacteria Undergoing Programmed Population Control in a
Microchemostat; Science; Vol. 309; No. 5731; 137-140; 10.1126/science.1109173
- Udit, Andrew K.; Hindoyan, Nareen; et el. (2005) Protein−Surfactant
Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3;
Inorganic Chemistry; Vol. 44; No. 12; 4109-4111; 10.1021/ic0483747
- Basu, Subhayu; Gerchman, Yoram; et el. (2005) A
synthetic multicellular system for programmed pattern formation;
Nature; Vol. 434; No. 7037; 1130-1134; 10.1038/nature03461
- Drummond, D. Allan; Silberg, Jonathan J.; et el. (2005) On the
conservative nature of intragenic recombination; Proceedings of the
National Academy of Sciences of the United States of America; Vol. 102;
No. 15; 5380-5385; PMCID PMC556249; 10.1073/pnas.0500729102
- Münzer, Dieter F.; Meinhold, Peter; et el. (2005) Stereoselective
hydroxylation of an achiral cyclopentanecarboxylic acid derivative using
engineered P450s BM-3; Chemical Communications; Vol. 2005; No. 20;
2597-2599; 10.1039/b501527h
- Umeno, Daisuke; Tobias, Alexander V.; et el. (2005) Diversifying
Carotenoid Biosynthetic Pathways by Directed Evolution; Microbiology
and Molecular Biology Reviews; Vol. 69; No. 1; 51-78; PMCID PMC1082795;
10.1128/MMBR.69.1.51-78.2005
- Collins, Cynthia H.; Arnold, Frances H.; et el. (2005) Directed
evolution of Vibrio fischeri LuxR for increased sensitivity to a broad
spectrum of acyl-homoserine lactones; Molecular Microbiology; Vol.
55; No. 3; 712-723; 10.1111/j.1365-2958.2004.04437.x
- Bloom, Jesse D.; Silberg, Jonathan J.; et el. (2005) Thermodynamic
prediction of protein neutrality; Proceedings of the National
Academy of Sciences of the United States of America; Vol. 102; No. 3;
606-611; PMCID PMC545518; 10.1073/pnas.0406744102
- Malliaros, Jim; Quinn, Charles; et el. (2004) Association
of antigens to ISCOMATRIX™ adjuvant using metal chelation leads to
improved CTL responses; Vaccine; Vol. 22; No. 29-30; 3968-3975; 10.1016/j.vaccine.2004.03.054
- Udit, Andrew K.; Arnold, Frances H.; et el. (2004) Cobaltocene-mediated
catalytic monooxygenation using holo and heme domain cytochrome P450
BM3; Journal of Inorganic Biochemistry; Vol. 98; No. 9; 1547-1550;
10.1016/j.jinorgbio.2004.06.007
- Udit, Andrew K.; Hill, Michael G.; et el. (2004) Reduction
of Dioxygen Catalyzed by Pyrene-Wired Heme Domain Cytochrome P450 BM3
Electrodes; Journal of the American Chemical Society; Vol. 126;
No. 33; 10218-10219; 10.1021/ja0466560
- Endelman, Jeffrey B.; Silberg, Jonathan J.; et el. (2004) Site-directed
protein recombination as a shortest-path problem; Protein
Engineering, Design and Selection; Vol. 17; No. 7; 589-594; 10.1093/protein/gzh067
- Bloom, Jesse D.; Wilke, Claus O.; et el. (2004) Stability
and the Evolvability of Function in a Model Protein; Biophysical
Journal; Vol. 86; No. 5; 2758-2764; PMCID PMC1304146; 10.1016/S0006-3495(04)74329-5
- You, Lingchong; Cox, Robert Sidney, III; et el. (2004) Programmed
population control by cell-cell communication and regulated killing;
Nature; Vol. 428; No. 6985; 868-871; 10.1038/nature02491
- Alcalde, Miguel; Farinas, Edgardo T.; et el. (2004) Colorimetric
High-Throughput Assay for Alkene Epoxidation Catalyzed by Cytochrome
P450 BM-3 Variant 139-3; Journal of Biomolecular Screening; Vol. 9;
No. 2; 141-146; 10.1177/1087057103261913
- Umeno, Daisuke and Arnold, Frances H. (2004) Evolution
of a Pathway to Novel Long-Chain Carotenoids; Journal of
Bacteriology; Vol. 186; No. 5; 1531-1536; PMCID PMC344396; 10.1128/JB.186.5.1531-1536.2004
- Otey, Christopher R.; Silberg, Jonathan J.; et el. (2004) Functional
Evolution and Structural Conservation in Chimeric Cytochromes P450:
Calibrating a Structure-Guided Approach; Chemistry and Biology; Vol.
11; No. 3; 309-318; 10.1016/j.chembiol.2004.02.018
- Wong, Tuck Seng; Arnold, Frances H.; et el. (2004) Laboratory
evolution of cytochrome P450 BM-3 monooxygenase for organic
cosolvents; Biotechnology and Bioengineering; Vol. 85; No. 3;
351-358; 10.1002/bit.10896
- Peters, Matthew W.; Meinhold, Peter; et el. (2003) Regio-
and Enantioselective Alkane Hydroxylation with Engineered Cytochromes
P450 BM-3; Journal of the American Chemical Society; Vol. 125;
No. 44; 13442-13450; 10.1021/ja0303790
- Cirino, Patrick C.; Tang, Yi; et el. (2003) Global
incorporation of norleucine in place of methionine in cytochrome P450
BM-3 heme domain increases peroxygenase activity; Biotechnology and
Bioengineering; Vol. 83; No. 6; 729-734; 10.1002/bit.10718
- Salazar, Oriana; Cirino, Patrick C.; et el. (2003) Thermostabilization
of a Cytochrome P450 Peroxygenase; ChemBioChem; Vol. 4; No. 9;
891-893; 10.1002/cbic.200300660
- Umeno, Daisuke; Hiraga, Kaori; et el. (2003) Method to
protect a targeted amino acid residue during random mutagenesis;
Nucleic Acids Research; Vol. 31; No. 16; e91; PMCID PMC169983; 10.1093/nar/gng091
- Collins, Cynthia H.; Yokobayashi, Yohei; et el. (2003) Engineering
proteins that bind, move, make and break DNA; Current Opinion in
Biotechnology; Vol. 14; No. 4; 371-378; 10.1016/S0958-1669(03)00091-0
- Meyer, Michelle M.; Silberg, Jonathan J.; et el. (2003) Library
analysis of SCHEMA-guided protein recombination; Protein Science;
Vol. 12; No. 8; 1686-1693; PMCID PMC2323955; 10.1110/ps.0306603
- Cirino, Patrick C. and Arnold, Frances H. (2003) A
Self-Sufficient Peroxide-Driven Hydroxylation Biocatalyst;
Angewandte Chemie International Edition; Vol. 42; No. 28; 3299-3301; 10.1002/anie.200351434
- Hiraga, Kaori and Arnold, Frances H. (2003) General
Method for Sequence-independent Site-directed Chimeragenesis;
Journal of Molecular Biology; Vol. 330; No. 2; 287-296; 10.1016/S0022-2836(03)00590-4
- Umeno, Daisuke and Arnold, Frances H. (2003) A C35
Carotenoid Biosynthetic Pathway; Applied and Environmental
Microbiology; Vol. 69; No. 6; 3573-3579; PMCID PMC161527; 10.1128/AEM.69.6.3573-3579.2003
- Wintrode, Patrick L.; Zhang, Deqiang; et el. (2003) Protein
Dynamics in a Family of Laboratory Evolved Thermophilic Enzymes;
Journal of Molecular Biology; Vol. 327; No. 3; 745-757; 10.1016/S0022-2836(03)00147-5
- Yokobayashi, Yohei; Collins, Cynthia H.; et el. (2003) Evolutionary
Design Of Genetic Circuits And Cell-Cell Communications; Advances in
Complex Systems; Vol. 6; No. 1; 37-45; 10.1142/S0219525903000700
- Bulter, Thomas; Alcalde, Miguel; et el. (2003) Functional
Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed
Evolution; Applied and Environmental Microbiology; Vol. 69; No. 2;
987-995; PMCID PMC143632; 10.1128/AEM.69.2.987-995.2003
- Yokobayashi, Yohei; Weiss, Ron; et el. (2002) Directed
evolution of a genetic circuit; Proceedings of the National Academy
of Sciences of the United States of America; Vol. 99; No. 26;
16587-16591; PMCID PMC139187; 10.1073/pnas.252535999
- Alcalde, Miguel; Bulter, Thomas; et el. (2002) Colorimetric
Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal
Laccases; Journal of Biomolecular Screening; Vol. 7; No. 6; 547-553;
10.1177/1087057102238629
- Umeno, Daisuke; Tobias, Alexander V.; et el. (2002) Evolution
of the C30 Carotenoid Synthase CrtM for Function in a C40 Pathway;
Journal of Bacteriology; Vol. 184; No. 23; 6690-6699; PMCID PMC135437;
10.1128/JB.184.23.6690-6699.2002
- Glieder, Anton; Farinas, Edgardo T.; et el. (2002) Laboratory
evolution of a soluble, self-sufficient, highly active alkane
hydroxylase; Nature Biotechnology; Vol. 20; No. 11; 1135-1139; 10.1038/nbt744
- Cirino, Patrick C. and Arnold, Frances H. (2002) Regioselectivity
and Activity of Cytochrome P450 BM-3 and Mutant F87A in Reactions Driven
by Hydrogen Peroxide; Advanced Synthesis and Catalysis; Vol. 344;
No. 9; 932-937; 10.1002/1615-4169(200210)344:9<932::AID-ADSC932>3.0.CO;2-M
- Sun, Lianhong; Bulter, Thomas; et el. (2002) Modification
of Galactose Oxidase to Introduce Glucose 6-Oxidase Activity;
ChemBioChem; Vol. 3; No. 8; 781-783; 10.1002/1439-7633(20020802)3:8<781::AID-CBIC781>3.0.CO;2-8
- Voigt, Christopher A.; Martinez, Carlos; et el. (2002) Protein
building blocks preserved by recombination; Nature Structural
Biology; Vol. 9; No. 7; 553-558; 10.1038/nsb805
- Fu, Anne Y.; Chou, Hou-Pu; et el. (2002) An
Integrated Microfabricated Cell Sorter; Analytical Chemistry; Vol.
74; No. 11; 2451-2457; 10.1021/ac0255330
- Mayer, Kimberly M. and Arnold, Frances H. (2002) A
Colorimetric Assay to Quantify Dehydrogenase Activity in Crude Cell
Lysates; Journal of Biomolecular Screening; Vol. 7; No. 2; 135-140;
10.1177/108705710200700206
- Cirino, Patrick C. and Arnold, Frances H. (2002) Protein
engineering of oxygenases for biocatalysis; Current Opinion in
Chemical Biology; Vol. 6; No. 2; 130-135; 10.1016/S1367-5931(02)00305-8
- Joern, John M; Meinhold, Peter; et el. (2002) Analysis
of shuffled gene libraries; Journal of Molecular Biology; Vol. 316;
No. 3; 643-656; 10.1006/jmbi.2001.5349
- Farinas, Edgardo T.; Bulter, Thomas; et el. (2001) Directed
enzyme evolution; Current Opinion in Biotechnology; Vol. 12; No. 6;
545-551; 10.1016/S0958-1669(01)00261-0
- Wintrode, Patrick L.; Miyazaki, Kentaro; et el. (2001) Patterns
of adaptation in a laboratory evolved thermophilic enzyme;
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular
Enzymology; Vol. 1549; No. 1; 1-8; 10.1016/S0167-4838(01)00226-6
- Sun, Lianhong; Petrounia, Ioanna P.; et el. (2001) Expression
and stabilization of galactose oxidase in Escherichia coli by directed
evolution; Protein Engineering; Vol. 14; No. 9; 699-704; 10.1093/protein/14.9.699
- Morawski, Birgit; Quan, Sara; et el. (2001) Functional
expression and stabilization of horseradish peroxidase by directed
evolution in Saccharomyces cerevisiae; Biotechnology and
Bioengineering; Vol. 76; No. 2; 99-107; 10.1002/bit.1149
- Sakamoto, Takeshi; Joern, John M.; et el. (2001) Laboratory
Evolution of Toluene Dioxygenase To Accept 4-Picoline as a
Substrate; Applied and Environmental Microbiology; Vol. 67; No. 9;
3882-3887; PMCID PMC93105; 10.1128/AEM.67.9.3882-3887.2001
- Joern, John M.; Sakamoto, Takeshi; et el. (2001) A
Versatile High Throughput Screen for Dioxygenase Activity Using
Solid-Phase Digital Imaging; Journal of Biomolecular Screening; Vol.
6; No. 4; 219-223; 10.1177/108705710100600403
- Farinas, Edgardo T.; Schwaneberg, Ulrich; et el. (2001) Directed
Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation;
Advanced Synthesis and Catalysis; Vol. 343; No. 6-7; 601-606; 10.1002/1615-4169(200108)343:6/7<601::AID-ADSC601>3.0.CO;2-9
- Sieber, Volker; Martinez, Carlos A.; et el. (2001) Libraries
of hybrid proteins from distantly related sequences; Nature
Biotechnology; Vol. 19; No. 5; 456-460; 10.1038/88129
- Thorsen, Todd; Roberts, Richard W.; et el. (2001) Dynamic
Pattern Formation in a Vesicle-Generating Microfluidic Device;
Physical Review Letters; Vol. 86; No. 18; 4163-4166; 10.1103/PhysRevLett.86.4163
- Schwaneberg, Ulrich; Otey, Christopher; et el. (2001) Cost-Effective
Whole-Cell Assay for Laboratory Evolution of Hydroxylases in Escherichia
coli; Journal of Biomolecular Screening; Vol. 6; No. 2; 111-117; 10.1177/108705710100600207
- Voigt, Christopher A.; Mayo, Stephen L.; et el. (2001) Computational
method to reduce the search space for directed protein evolution;
Proceedings of the National Academy of Sciences of the United States of
America; Vol. 98; No. 7; 3778-3783; PMCID PMC31129; 10.1073/pnas.051614498
- Arnold, Frances H. (2001) Combinatorial
and computational challenges for biocatalyst design; Nature; Vol.
409; No. 6817; 253-257; 10.1038/35051731
- Voigt, Christopher A.; Mayo, Stephen L.; et el. (2001) Computationally
Focusing the Directed Evolution of Proteins; Journal of Cellular
Biochemistry; Vol. 84; No. S37; 58-63; 10.1002/jcb.10066
- Wintrode, Patrick L.; Miyazaki, Kentaro; et el. (2000) Cold
Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory
Evolution; Journal of Biological Chemistry; Vol. 275; No. 41;
31635-31640; 10.1074/jbc.M004503200
- Petrounia, Ioanna P. and Arnold, Frances H. (2000) Designed
evolution of enzymatic properties; Current Opinion in Biotechnology;
Vol. 11; No. 4; 325-330; 10.1016/s0958-1669(00)00107-5
- Schmidt-Dannert, Claudia; Umeno, Daisuke; et el. (2000) Molecular
breeding of carotenoid biosynthetic pathways; Nature Biotechnology;
Vol. 18; No. 7; 750-753; 10.1038/77319
- Morawski, Birgit; Lin, Zhanglin; et el. (2000) Functional
expression of horseradish peroxidase in Saccharomyces cerevisiae and
Pichia pastoris; Protein Engineering; Vol. 13; No. 5; 377-384; 10.1093/protein/13.5.377
- Gershenson, Anne; Schauerte, Joseph A.; et el. (2000) Tryptophan
Phosphorescence Study of Enzyme Flexibility and Unfolding in
Laboratory-Evolved Thermostable Esterases; Biochemistry; Vol. 39;
No. 16; 4658-4665; 10.1021/bi992473s
- Miyazaki, Kentaro; Wintrode, Patrick L.; et el. (2000) Directed
evolution study of temperature adaptation in a psychrophilic enzyme;
Journal of Molecular Biology; Vol. 297; No. 4; 1015-1026; 10.1006/jmbi.2000.3612
- May, Oliver; Nguyen, Peter T.; et el. (2000) Inverting
enantioselectivity by directed evolution of hydantoinase for improved
production of L-methionine; Nature Biotechnology; Vol. 18; No. 3;
317-320; 10.1038/73773
- Michnick, Stephen W. and Arnold, Frances H. (1999) “Itching”
for new strategies in protein engineering; Nature Biotechnology;
Vol. 17; No. 12; 1159-1160; 10.1038/70699
- Miyazaki, Kentaro and Arnold, Frances H. (1999) Exploring
Nonnatural Evolutionary Pathways by Saturation Mutagenesis: Rapid
Improvement of Protein Function; Journal of Molecular Evolution;
Vol. 49; No. 6; 716-720; 10.1007/pl00006593
- Gillam, Elizabeth M. J.; Aguinaldo, Anna Marie A.; et el. (1999) Formation
of Indigo by Recombinant Mammalian Cytochrome P450; Biochemical and
Biophysical Research Communications; Vol. 265; No. 2; 469-472; 10.1006/bbrc.1999.1702
- Fu, Anne Y.; Spence, Charles; et el. (1999) A
microfabricated fluorescence-activated cell sorter; Nature
Biotechnology; Vol. 17; No. 11; 1109-1111; 10.1038/15095
- Spiller, Ben; Gershenson, Anne; et el. (1999) A
structural view of evolutionary divergence; Proceedings of the
National Academy of Sciences of the United States of America; Vol. 96;
No. 22; 12305-12310; PMCID PMC22912
- Joo, Hyun; Arisawa, Akira; et el. (1999) A
high-throughput digital imaging screen for the discovery and directed
evolution of oxygenases; Chemistry and Biology; Vol. 6; No. 10;
699-706; 10.1016/S1074-5521(00)80017-4
- Volkov, Alexander A.; Shao, Zhixin; et el. (1999) Recombination
and chimeragenesis by in vitro heteroduplex formation and in vivo
repair; Nucleic Acids Research; Vol. 27; No. 18; e18; PMCID
PMC148610; 10.1093/nar/27.18.e18
- Joo, Hyun; Lin, Zhanglin; et el. (1999) Laboratory
evolution of peroxide-mediated cytochrome P450 hydroxylation;
Nature; Vol. 399; No. 6737; 670-673; 10.1038/21395
- Arnold, Frances H.; Giver, Lori; et el. (1999) Directed
Evolution of Mesophilic Enzymes into Their Thermophilic
Counterparts; Annals of the New York Academy of Sciences; Vol. 870;
No. 1; 400-403; 10.1111/j.1749-6632.1999.tb08913.x
- Lin, Zhanglin; Thorsen, Todd; et el. (1999) Functional
Expression of Horseradish Peroxidase in E. coli by Directed
Evolution; Biotechnology Progress; Vol. 15; No. 3; 467-471; 10.1021/bp990037r
- Schmidt-Dannert, Claudia and Arnold, Frances H. (1999) Directed
evolution of industrial enzymes; Trends in Biotechnology; Vol. 17;
No. 4; 135-136; 10.1016/s0167-7799(98)01283-9
- Arnold, Frances H. and Volkov, Alexander A. (1999) Directed
evolution of biocatalysts; Current Opinion in Chemical Biology; Vol.
3; No. 1; 54-59; 10.1016/S1367-5931(99)80010-6
- Zhao, Huimin and Arnold, Frances H. (1999) Directed
evolution converts subtilisin E into a functional equivalent of
thermitase; Protein Engineering; Vol. 12; No. 1; 47-53; 10.1093/protein/12.1.47
- Joo, Hyun; Lin, Zhanglin; et el. (1999) Laboratory
evolution of peroxide-mediated cytochrome P450 hydroxylation; Redox
Report; Vol. 4; No. 5; 195-197; 10.1179/135100099101534918
- Giver, Lori; Gershenson, Anne; et el. (1998) Directed
evolution of a thermostable esterase; Proceedings of the National
Academy of Sciences of the United States of America; Vol. 95; No. 22;
12809-12813; PMCID PMC23604
- Arnold, Frances H. (1998) When
blind is better: Protein design by evolution; Nature Biotechnology;
Vol. 16; No. 7; 617-618; 10.1038/nbt0798-617
- Giver, Lori and Arnold, Frances H. (1998) Combinatorial
protein design by in vitro recombination; Current Opinion in
Chemical Biology; Vol. 2; No. 3; 335-338; 10.1016/S1367-5931(98)80006-9
- Arnold, Frances H. (1998) Enzyme
engineering reaches the boiling point; Proceedings of the National
Academy of Sciences of the United States of America; Vol. 95; No. 5;
2035-2036; PMCID PMC33840
- Arnold, Frances H. (1998) Design
by Directed Evolution; Accounts of Chemical Research; Vol. 31;
No. 3; 125-131; 10.1021/ar960017f
- Zhao, Huimin; Giver, Lori; et el. (1998) Molecular
evolution by staggered extension process (StEP) in vitro
recombination; Nature Biotechnology; Vol. 16; No. 3; 258-261; 10.1038/nbt0398-258
- Shao, Zhixin; Zhao, Huimin; et el. (1998) Random-priming
in vitro recombination: an effective tool for directed evolution;
Nucleic Acids Research; Vol. 26; No. 2; 681-683; PMCID PMC147287; 10.1093/nar/26.2.681
- Kuchner, Olga and Arnold, Frances H. (1997) Directed
evolution of enzyme catalysts; Trends in Biotechnology; Vol. 15;
No. 12; 523-530; 10.1016/S0167-7799(97)01138-4
- Moore, Jeffrey C.; Jin, Hua-Ming; et el. (1997) Strategies
for the in vitro evolution of protein function: enzyme evolution by
random recombination of improved sequences; Journal of Molecular
Biology; Vol. 272; No. 3; 336-347; 10.1006/jmbi.1997.1252
- Zhao, Huimin and Arnold, Frances H. (1997) Combinatorial
protein design: strategies for screening protein libraries; Current
Opinion in Structural Biology; Vol. 7; No. 4; 480-485; 10.1016/S0959-440X(97)80110-8
- Zhao, Huimin and Arnold, Frances H. (1997) Functional
and nonfunctional mutations distinguished by random recombination of
homologous genes; Proceedings of the National Academy of Sciences of
the United States of America; Vol. 94; No. 15; 7997-8000; PMCID
PMC21544
- Pack, Daniel W. and Arnold, Frances H. (1997) Langmuir
monolayer characterization of metal chelating lipids for protein
targeting to membranes; Chemistry and Physics of Lipids; Vol. 86;
No. 2; 135-152; 10.1016/s0009-3084(97)02662-5
- Chen, G.; Guan, Z.; et el. (1997) A
glucose sensing polymer; Abstracts of Papers of the American
Chemical Society; Vol. 213; 176-ANYL
- Chen, G.; Guan, Z.; et el. (1997) A
glucose-sensing polymer; Abstracts of Papers of the American
Chemical Society; Vol. 213; 234-PMSE
- Chen, Guohua; Guan, Zhibin; et el. (1997) A
glucose-sensing polymer; Nature Biotechnology; Vol. 15; No. 4;
354-357; 10.1038/nbt0497-354
- Zhao, Huimin and Arnold, Frances H. (1997) Optimization
of DNA shuffling for high fidelity recombination; Nucleic Acids
Research; Vol. 25; No. 6; 1307-1308; PMCID PMC146579; 10.1093/nar/25.6.1307
- Pack, Daniel W.; Chen, Guohua; et el. (1997) A
Metal-Chelating Lipid for 2D Protein Crystallization via Coordination of
Surface Histidines; Journal of the American Chemical Society; Vol.
119; No. 10; 2479-2487; 10.1021/ja964099e
- Zhao, Huimin; Li, You; et el. (1996) Strategy
for the Directed Evolution of a Peptide Ligase; Annals of the New
York Academy of Sciences; Vol. 799; No. 1; 1-5; 10.1111/j.1749-6632.1996.tb33169.x
- Shao, Zhixin and Arnold, Frances H. (1996) Engineering
new functions and altering existing functions; Current Opinion in
Structural Biology; Vol. 6; No. 4; 513-518; 10.1016/S0959-440X(96)80117-5
- Frey, Wolfgang; Schief, William R., Jr.; et el. (1996) Two-dimensional
protein crystallization via metal-ion coordination by naturally
occurring surface histidines; Proceedings of the National Academy of
Sciences of the United States of America; Vol. 93; No. 10; 4937-4941;
PMCID PMC39383
- Moore, Jeffrey C. and Arnold, Frances H. (1996) Directed
evolution of a para-nitrobenzyl esterase for aqueous-organic
solvents; Nature Biotechnology; Vol. 14; No. 4; 458-467; 10.1038/nbt0496-458
- Johnson, R. D.; Wang, Z.-G.; et el. (1996) Surface
Site Heterogeneity and Lateral Interactions in Multipoint Protein
Adsorption; Journal of Physical Chemistry; Vol. 100; No. 12;
5134-5139; 10.1021/jp9523682
- Johnson, Robert D.; Todd, Robert J.; et el. (1996) Multipoint
binding in metal-affinity chromatography: II. Effect of pH and imidazole
on chromatographic retention of engineered histidine-containing
cytochromes c; Journal of Chromatography A; Vol. 725; No. 2;
225-235; 10.1016/0021-9673(95)00992-2
- You, L. and Arnold, F. H. (1996) Directed
evolution of subtilisin E in Bacillus subtilis to enhance total activity
in aqueous dimethylformamide; Protein Engineering; Vol. 9; No. 1;
77-83; 10.1093/protein/9.1.77
- Johnson, Robert D. and Arnold, Frances H. (1995) Review:
Multipoint binding and heterogeneity in immobilized metal affinity
chromatography; Biotechnology and Bioengineering; Vol. 48; No. 5;
437-443; 10.1002/bit.260480505
- Vidyasankar, Sundaresan; Dhal, Pradeep K.; et el. (1995) Review:
Selective ligand-exchange adsorbents prepared by template
polymerization; Biotechnology and Bioengineering; Vol. 48; No. 5;
431-436; 10.1002/bit.260480504
- Ng, Kingman; Pack, Daniel W.; et el. (1995) Engineering
Protein-Lipid Interactions: Targeting of Histidine-Tagged Proteins to
Metal-Chelating Lipid Monolayers; Langmuir; Vol. 11; No. 10;
4048-4055; 10.1021/la00010a069
- Plunkett, Sean D. and Arnold, Frances H. (1995) Molecularly
imprinted polymers on silica: selective supports for high-performance
ligand-exchange chromatography; Journal of Chromatography A; Vol.
708; No. 1; 19-29; 10.1016/0021-9673(95)00378-Z
- Sasaki, Darryl Y.; Shnek, Deborah R.; et el. (1995) Metal-Induced
Dispersion of Lipid Aggregates: A Simple, Selective, and Sensitive
Fluorescent Metal Ion Sensor; Angewandte Chemie International
Edition; Vol. 34; No. 8; 905-907; 10.1002/anie.199509051
- Johnson, Robert D. and Arnold, Frances H. (1995) The
temkin isotherm describes heterogeneous protein adsorption;
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular
Enzymology; Vol. 1247; No. 2; 293-297; 10.1016/0167-4838(95)00006-G
- Shnek, D. R.; Pack, D. W.; et el. (1994) Specific
Protein Attachment to Artificial Membranes via Coordination to
Lipid-Bound Copper(II); Langmuir; Vol. 10; No. 7; 2382-2388; 10.1021/la00019a058
- Arnold, Frances H. and Zhang, Ji-Hu (1994) Metal-mediated
protein stabilization; Trends in Biotechnology; Vol. 12; No. 5;
189-192; 10.1016/0167-7799(94)90081-7
- Todd, Robert J.; Johnson, Robert D.; et el. (1994) Multiple-site
binding interactions in metal-affinity chromatography: I. Equilibrium
binding of engineered histidine-containing cytochromes c; Journal of
Chromatography A; Vol. 662; No. 1; 13-26; 10.1016/0021-9673(94)85291-x
- Arnold, Frances H. (1993) Protein
engineering for unusual environments; Current Opinion in
Biotechnology; Vol. 4; No. 4; 450-455; 10.1016/0958-1669(93)90011-k
- Arnold, F. H. (1993) Engineering
proteins for nonnatural environments; FASEB Journal; Vol. 7; No. 9;
744-749; 10.1096/fasebj.7.9.8330682
- Chen, Keqin and Arnold, Frances H. (1993) Tuning the
Activity of an Enzyme for Unusual Environments: Sequential Random
Mutagenesis of Subtilisin E for Catalysis in Dimethylformamide;
Proceedings of the National Academy of Sciences of the United States of
America; Vol. 90; No. 6; 5618-5622; PMCID PMC46772
- Mallik, Sanku; Johnson, Robert D.; et el. (1993) Selective
recognition of bis-imidazoles by complementary bis-metal ion
complexes; Journal of the American Chemical Society; Vol. 115;
No. 6; 2518-2520; 10.1021/ja00059a063
- Dhal, Pradeep K. and Arnold, Frances H. (1992) Metal-coordination
interactions in the template-mediated synthesis of substrate-selective
polymers: recognition of bis(imidazole) substrates by copper(II)
iminodiacetate containing polymers; Macromolecules; Vol. 25; No. 25;
7051-7059; 10.1021/ma00051a050
- Economou, Chariklia; Chen, Keqin; et el. (1992) Random
mutagenesis to enhance the activity of subtilisin in organic solvents:
Characterization of Q103R subtilisin E; Biotechnology and
Bioengineering; Vol. 39; No. 6; 658-662; 10.1002/bit.260390610
- Martinez, Pascal; Van Dam, Mariana E.; et el. (1992) Stabilization
of substilisin E in organic solvents by site-directed mutagenesis;
Biotechnology and Bioengineering; Vol. 39; No. 2; 141-147; 10.1002/bit.260390204
- Martinez, Pascal; Van Dam, Mariana E.; et el. (1992) Stabilization
of substilisin E in organic solvents by site‐directed mutagenesis;
Biotechnology and Bioengineering; Vol. 39; No. 2; 141-147; 10.1002/bit.260390204
- Chen, Keqin and Arnold, Frances H. (1991) Enzyme
Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance
Activity of Subtilisin E in Polar Organic Media; Bio/Technology;
Vol. 9; No. 11; 1073-1077; 10.1038/nbt1191-1073
- Kellis, James T., Jr.; Todd, Robert J.; et el. (1991) Protein
Stabilization by Engineered Metal Chelation; Bio/Technology; Vol. 9;
No. 10; 994-995; 10.1038/nbt1091-994
- Dhal, Pradeep K. and Arnold, Frances H. (1991) Template-mediated
synthesis of metal-complexing polymers for molecular recognition;
Journal of the American Chemical Society; Vol. 113; No. 19; 7417-7418;
10.1021/ja00019a046
- Arnold, Frances H. and Haymore, Barry L. (1991) Engineered
metal-binding proteins: purification to protein folding; Science;
Vol. 252; No. 5014; 1796-1797; 10.1126/science.1648261
- Chen, Keqin; Robinson, Amy C.; et el. (1991) Enzyme
engineering for nonaqueous solvents. II. Additive effects of mutations
on the stability and activity of subtilisin E in polar organic
media; Biotechnology Progress; Vol. 7; No. 2; 125-129; 10.1021/bp00008a007
- Suh, Sung-Sup; Haymore, Barry L.; et el. (1991) Characterization
of His-X_3-His sites in α-helices of synthetic metal-binding bovine
somatotropin; Protein Engineering, Design and Selection; Vol. 4;
No. 3; 301-305; 10.1093/protein/4.3.301
- Todd, Robert J.; Van Dam, Mariana E.; et el. (1991) Cu(II)-Binding
properties of a cytochrome c with a synthetic metal-binding site:
His-X_3-His in an α-helix; Proteins; Vol. 10; No. 2; 156-161; 10.1002/prot.340100209
- Arnold, Frances H. (1991) Metal-Affinity
Separations: A New Dimension in Protein Processing; Bio/Technology;
Vol. 9; No. 2; 151-156; 10.1038/nbt0291-151
- Wuenschell, G. E.; Wen, E.; et el. (1991) Chiral
copper—chelate complexes alter selectivities in metal affinity protein
partitioning; Journal of Chromatography A; Vol. 543; 345-354; 10.1016/S0021-9673(01)95786-3
- Arnold, Frances H. (1990) Engineering
enzymes for non-aqueous solvents; Trends in Biotechnology; Vol. 8;
No. 9; 244-249; 10.1016/0167-7799(90)90186-2
- Suh, Sung-Sup and Arnold, Frances H. (1990) A
mathematical model for metal affinity protein partitioning;
Biotechnology and Bioengineering; Vol. 35; No. 7; 682-690; 10.1002/bit.260350705
- Claeys, Ivan L. and Arnold, Frances H. (1989) Nuclear
magnetic relaxation study of hindered rotational diffusion in gels;
AIChE Journal; Vol. 35; No. 2; 335-338; 10.1002/aic.690350223
- Arnold, Frances H.; Wolk, Steven; et el. (1987) Structure,
dynamics, and thermodynamics of mismatched DNA oligonucleotide duplexes
d(CCCAGGG)_2 and d(CCCTGGG)_2; Biochemistry; Vol. 26; No. 13;
4068-4075; 10.1021/bi00387a049
- Arnold, F. H.; Schofield, S. A.; et el. (1986) Analytical
affinity chromatography: I. Local equilibrium theory and the measurement
of association and inhibition constants; Journal of Chromatography
A; Vol. 355; 1-12; 10.1016/s0021-9673(01)97299-1
- Arnold, F. H. and Blanch, H. W. (1986) Analytical
affinity chromatography: II. Rate theory and the measurement of
biological binding kinetics; Journal of Chromatography A; Vol. 355;
13-27; 10.1016/S0021-9673(01)97300-5